Rmu_sc0005664.1_g000044

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0005664.1
OG0013736
Physical Location & Seq
Reverse (-)
143272 .. 145150
1879 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0005664.1_g000044.1.cds

Sequence Viewer

Length: 546 bp
atggatgttgcacgtggctcagagcactacccacccggcgaccatgcgaacagcgagttatgcgtcgcgcacacgattgggaactctgccagagcctctggtacgctcgctacctgcatcatccaacccatcgatatgataaagatctatgatttgtttggcttcgttgatagaggagaggggggctttgaaatggaggaagaacaaggatgttgttctgtggtttgggttgtctctgtttctgctttttcctcttgcttcatcactgaacagaatttgtccagcttgaatcttggcgaagagatttcgccagcccttggagatttgggaaacttggaatccatgaatttatctgacaataatccgtctggtgacatacccttctcggtttcaaagcttaagaaccgtgaggttttgaatttgaagaacaatcagttggctggtcctatcgctacaactcttacccaaattccaaaccttagaactcttggattgcgaggcaactctttgactggagcactgtcccctgatgtgtcaattaactag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

181

Amino Acids

19.24

Weight (kDa)

4.5

Isoelectric Point (pI)

31.57

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 122
AccB7I CCANNNNNTGG 1 cut(s) 317
AccII CGCG 1 cut(s) 68
AcsI RAATTY 4 cut(s) 274, 346, 418, 468
AcvI CACGTG 1 cut(s) 14
AfaI GTAC 1 cut(s) 103
AfiI CCNNNNNNNGG 1 cut(s) 317
AflII CTTAAG 1 cut(s) 398
AgsI TTSAA 5 cut(s) 191, 289, 393, 418, 424
AjuI GAANNNNNNNTTGG 2 cut(s) 419, 451
AluBI AGCT 2 cut(s) 285, 397
AluI AGCT 2 cut(s) 285, 397
Alw21I GWGCWC 2 cut(s) 27, 520
Alw26I GTCTC 1 cut(s) 238
ApoI RAATTY 4 cut(s) 274, 346, 418, 468
AspLEI GCGC 1 cut(s) 70
AspS9I GGNCC 1 cut(s) 443
AsuC2I CCSGG 1 cut(s) 36
AsuHPI GGTGA 1 cut(s) 383
AvaII GGWCC 1 cut(s) 443
BbrPI CACGTG 1 cut(s) 14
Bbv12I GWGCWC 2 cut(s) 27, 520
BccI CCATC 1 cut(s) 137
BcnI CCSGG 1 cut(s) 36
BcoDI GTCTC 1 cut(s) 238
BfaI CTAG 1 cut(s) 544
BfrI CTTAAG 1 cut(s) 398
BfuAI ACCTGC 1 cut(s) 122
BglII AGATCT 1 cut(s) 144
Bme1390I CCNGG 1 cut(s) 36
Bme18I GGWCC 1 cut(s) 443
BmgT120I GGNCC 1 cut(s) 443
BmrFI CCNGG 1 cut(s) 36
BmsI GCATC 1 cut(s) 126
BpmI CTGGAG 1 cut(s) 534
BpuMI CCSGG 1 cut(s) 36
Bsa29I ATCGAT 1 cut(s) 132
BsaAI YACGTR 1 cut(s) 14
BsaBI GATNNNNATC 1 cut(s) 143
BsaJI CCNNGG 1 cut(s) 316
Bsc4I CCNNNNNNNGG 1 cut(s) 317
Bse1I ACTGG 1 cut(s) 517
Bse8I GATNNNNATC 1 cut(s) 143
BseCI ATCGAT 1 cut(s) 132
BseDI CCNNGG 1 cut(s) 316
BseGI GGATG 3 cut(s) 10, 120, 215
BseJI GATNNNNATC 1 cut(s) 143
BseLI CCNNNNNNNGG 1 cut(s) 317
BseMII CTCAG 1 cut(s) 33
BseNI ACTGG 1 cut(s) 517
BseRI GAGGAG 1 cut(s) 189
Bsh1236I CGCG 1 cut(s) 68
BshVI ATCGAT 1 cut(s) 132
BsiHKAI GWGCWC 2 cut(s) 27, 520
BsiSI CCGG 1 cut(s) 36
BslFI GGGAC 1 cut(s) 508
BslI CCNNNNNNNGG 1 cut(s) 317
BsmAI GTCTC 1 cut(s) 238
BsmFI GGGAC 1 cut(s) 508
Bsp1286I GDGCHC 2 cut(s) 27, 520
Bsp143I GATC 1 cut(s) 144
BspCNI CTCAG 1 cut(s) 32
BspDI ATCGAT 1 cut(s) 132
BspFNI CGCG 1 cut(s) 68
BspMI ACCTGC 1 cut(s) 122
BspTI CTTAAG 1 cut(s) 398
BsrI ACTGG 1 cut(s) 517
BssECI CCNNGG 1 cut(s) 316
BssMI GATC 1 cut(s) 144
BssT1I CCWWGG 1 cut(s) 316
Bst4CI ACNGT 2 cut(s) 407, 522
Bst6I CTCTTC 1 cut(s) 294
BstAFI CTTAAG 1 cut(s) 398
BstBAI YACGTR 1 cut(s) 14
BstC8I GCNNGC 2 cut(s) 108, 312
BstDEI CTNAG 2 cut(s) 19, 479
BstF5I GGATG 3 cut(s) 10, 120, 215
BstFNI CGCG 1 cut(s) 68
BstHHI GCGC 1 cut(s) 70
BstKTI GATC 1 cut(s) 147
BstMAI GTCTC 1 cut(s) 238
BstMBI GATC 1 cut(s) 144
BstMWI GCNNNNNNNGC 1 cut(s) 60
BstSCI CCNGG 1 cut(s) 34
BstUI CGCG 1 cut(s) 68
BstX2I RGATCY 1 cut(s) 144
BstYI RGATCY 1 cut(s) 144
Bsu15I ATCGAT 1 cut(s) 132
BsuTUI ATCGAT 1 cut(s) 132
BtsCI GGATG 3 cut(s) 10, 120, 215
BtsIMutI CAGTG 2 cut(s) 264, 518
BveI ACCTGC 1 cut(s) 122
Cac8I GCNNGC 2 cut(s) 108, 312
CfoI GCGC 1 cut(s) 70
Cfr13I GGNCC 1 cut(s) 443
ClaI ATCGAT 1 cut(s) 132
CseI GACGC 1 cut(s) 52
Csp6I GTAC 1 cut(s) 102
CviAII CATG 2 cut(s) 44, 343
CviJI RGCY 8 cut(s) 18, 95, 162, 186, 285, 314, 397, 440
CviKI_1 RGCY 8 cut(s) 18, 95, 162, 186, 285, 314, 397, 440
CviQI GTAC 1 cut(s) 102
DdeI CTNAG 2 cut(s) 19, 479
DpnI GATC 1 cut(s) 146
DpnII GATC 1 cut(s) 144
Eam1104I CTCTTC 1 cut(s) 294
EarI CTCTTC 1 cut(s) 294
Eco130I CCWWGG 1 cut(s) 316
Eco47I GGWCC 1 cut(s) 443
Eco72I CACGTG 1 cut(s) 14
EcoT14I CCWWGG 1 cut(s) 316
ErhI CCWWGG 1 cut(s) 316
FaeI CATG 2 cut(s) 47, 346
FaiI YATR 6 cut(s) 45, 61, 137, 150, 344, 377
FaqI GGGAC 1 cut(s) 508
FatI CATG 2 cut(s) 43, 342
FokI GGATG 3 cut(s) 17, 107, 222
FspBI CTAG 1 cut(s) 544
GlaI GCGC 1 cut(s) 69
GsuI CTGGAG 1 cut(s) 534
HapII CCGG 1 cut(s) 36
HgaI GACGC 1 cut(s) 52
HhaI GCGC 1 cut(s) 70
Hin1II CATG 2 cut(s) 47, 346
Hin6I GCGC 1 cut(s) 68
HinP1I GCGC 1 cut(s) 68
HindIII AAGCTT 1 cut(s) 395
HinfI GANTC 2 cut(s) 289, 338
HpaII CCGG 1 cut(s) 36
HphI GGTGA 1 cut(s) 383
Hpy188I TCNGA 2 cut(s) 22, 355
Hpy99I CGWCG 1 cut(s) 68
HpyAV CCTTC 1 cut(s) 391
HpyCH4III ACNGT 2 cut(s) 407, 522
HpyCH4IV ACGT 1 cut(s) 13
HpyCH4V TGCA 2 cut(s) 11, 117
HpyF10VI GCNNNNNNNGC 1 cut(s) 60
HpyF3I CTNAG 2 cut(s) 19, 479
HpySE526I ACGT 1 cut(s) 13
Hsp92II CATG 2 cut(s) 47, 346
HspAI GCGC 1 cut(s) 68
Kzo9I GATC 1 cut(s) 144
LmnI GCTCC 1 cut(s) 515
LweI GCATC 1 cut(s) 126
MaeI CTAG 1 cut(s) 544
MaeII ACGT 1 cut(s) 13
MaeIII GTNAC 1 cut(s) 371
MalI GATC 1 cut(s) 146
MboI GATC 1 cut(s) 144
MboII GAAGA 3 cut(s) 212, 311, 436
MflI RGATCY 1 cut(s) 144
MhlI GDGCHC 2 cut(s) 27, 520
MluCI AATT 5 cut(s) 274, 346, 418, 468, 537
MmeI TCCRAC 1 cut(s) 148
MnlI CCTC 7 cut(s) 106, 167, 172, 190, 262, 403, 491
MseI TTAA 2 cut(s) 399, 540
MslI CAYNNNNRTG 1 cut(s) 134
MspCI CTTAAG 1 cut(s) 398
MspI CCGG 1 cut(s) 36
MspR9I CCNGG 1 cut(s) 36
MvnI CGCG 1 cut(s) 68
MwoI GCNNNNNNNGC 1 cut(s) 60
NciI CCSGG 1 cut(s) 36
NdeII GATC 1 cut(s) 144
NlaIII CATG 2 cut(s) 47, 346
NmuCI GTSAC 1 cut(s) 371
PfeI GAWTC 2 cut(s) 289, 338
PflMI CCANNNNNTGG 1 cut(s) 317
PmaCI CACGTG 1 cut(s) 14
PmlI CACGTG 1 cut(s) 14
Ppu21I YACGTR 1 cut(s) 14
PspCI CACGTG 1 cut(s) 14
PspPI GGNCC 1 cut(s) 443
PsuI RGATCY 1 cut(s) 144
RsaI GTAC 1 cut(s) 103
RsaNI GTAC 1 cut(s) 102
RseI CAYNNNNRTG 1 cut(s) 134
SaqAI TTAA 2 cut(s) 399, 540
Sau3AI GATC 1 cut(s) 144
Sau96I GGNCC 1 cut(s) 443
ScrFI CCNGG 1 cut(s) 36
SduI GDGCHC 2 cut(s) 27, 520
SetI ASST 6 cut(s) 16, 116, 287, 399, 414, 480
SfaNI GCATC 1 cut(s) 126
SinI GGWCC 1 cut(s) 443
SmiMI CAYNNNNRTG 1 cut(s) 134
SmlI CTYRAG 1 cut(s) 398
SmoI CTYRAG 1 cut(s) 398
Sse9I AATT 5 cut(s) 274, 346, 418, 468, 537
SspMI CTAG 1 cut(s) 544
StyD4I CCNGG 1 cut(s) 34
StyI CCWWGG 1 cut(s) 316
TaaI ACNGT 2 cut(s) 407, 522
TaiI ACGT 1 cut(s) 16
TaqI TCGA 1 cut(s) 132
TasI AATT 5 cut(s) 274, 346, 418, 468, 537
TfiI GAWTC 2 cut(s) 289, 338
Tru1I TTAA 2 cut(s) 399, 540
Tru9I TTAA 2 cut(s) 399, 540
TscAI CASTG 2 cut(s) 271, 525
TseFI GTSAC 1 cut(s) 371
Tsp45I GTSAC 1 cut(s) 371
TspDTI ATGAA 2 cut(s) 250, 359
TspGWI ACGGA 1 cut(s) 354
TspRI CASTG 2 cut(s) 271, 525
Van91I CCANNNNNTGG 1 cut(s) 317
Vha464I CTTAAG 1 cut(s) 398
VpaK11BI GGWCC 1 cut(s) 443
XapI RAATTY 4 cut(s) 274, 346, 418, 468
XspI CTAG 1 cut(s) 544
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.