Rroxscaffold_7G00199140

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0013736
Physical Location & Seq
Reverse (-)
45075760 .. 45077418
1659 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00199140.1

Sequence Viewer

Length: 363 bp
ATGCCATTGAGGAGCTCCGGCGCCTTCATCGCACGGATGTTGCGCGTGGCTCGCAACACCACCCGCCCGGCCACCATGCGAACAAGTGAGTTATGCGTCGCGCACGCGATTGGGAACTCGCGAGCCTCCGGTATGTTCACTACCTGCATCATCCAACCCATCGATATGATCAAGGAATTTGTCCAGCTTGAATCTTGGCGAAGAGATTTCGCCAGCCCTTGGAGATCCTTGGAATCCATGAATGTATCCGACAATAATCCGTCCGGAGACATACCCTTCTCGGTTTCAAAGCTTAAGAACCTTGAGCTCACTGGTGAGATACCAAGGCTAATATCTTGGAATGAAGTTTTGCAGTACCTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

120

Amino Acids

13.57

Weight (kDa)

9.29

Isoelectric Point (pI)

44.58

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 152
AccB1I GGYRCC 1 cut(s) 20
AccB7I CCANNNNNTGG 1 cut(s) 219
AccII CGCG 4 cut(s) 45, 101, 107, 121
AccIII TCCGGA 1 cut(s) 263
AciI CCGC 1 cut(s) 64
AclWI GGATC 1 cut(s) 219
AcoI YGGCCR 1 cut(s) 69
AcsI RAATTY 1 cut(s) 176
AcyI GRCGYC 1 cut(s) 21
AfaI GTAC 1 cut(s) 356
AfiI CCNNNNNNNGG 1 cut(s) 219
AflII CTTAAG 1 cut(s) 293
AgsI TTSAA 2 cut(s) 191, 288
AluBI AGCT 4 cut(s) 15, 187, 292, 307
AluI AGCT 4 cut(s) 15, 187, 292, 307
Alw21I GWGCWC 2 cut(s) 17, 309
Alw26I GTCTC 1 cut(s) 261
AlwI GGATC 1 cut(s) 219
AlwNI CAGNNNCTG 1 cut(s) 358
Aor13HI TCCGGA 1 cut(s) 263
AoxI GGCC 1 cut(s) 69
ApoI RAATTY 1 cut(s) 176
AspLEI GCGC 3 cut(s) 23, 45, 103
AsuC2I CCSGG 1 cut(s) 68
AsuHPI GGTGA 1 cut(s) 326
BanI GGYRCC 1 cut(s) 20
BanII GRGCYC 2 cut(s) 17, 309
Bbv12I GWGCWC 2 cut(s) 17, 309
BccI CCATC 1 cut(s) 167
BciVI GTATCC 1 cut(s) 256
BclI TGATCA 1 cut(s) 168
BcnI CCSGG 1 cut(s) 68
BcoDI GTCTC 1 cut(s) 261
BfoI RGCGCY 1 cut(s) 24
BfrI CTTAAG 1 cut(s) 293
BfuAI ACCTGC 1 cut(s) 152
BfuI GTATCC 1 cut(s) 256
Bme1390I CCNGG 1 cut(s) 68
BmiI GGNNCC 1 cut(s) 22
BmrFI CCNGG 1 cut(s) 68
BmsI GCATC 1 cut(s) 156
BpuEI CTTGAG 1 cut(s) 323
BpuMI CCSGG 1 cut(s) 68
Bsa29I ATCGAT 1 cut(s) 162
BsaHI GRCGYC 1 cut(s) 21
BsaJI CCNNGG 3 cut(s) 218, 228, 323
BsaWI WCCGGW 2 cut(s) 128, 263
Bsc4I CCNNNNNNNGG 1 cut(s) 219
Bse1I ACTGG 1 cut(s) 316
BseAI TCCGGA 1 cut(s) 263
BseCI ATCGAT 1 cut(s) 162
BseDI CCNNGG 3 cut(s) 218, 228, 323
BseGI GGATG 2 cut(s) 42, 150
BseLI CCNNNNNNNGG 1 cut(s) 219
BseNI ACTGG 1 cut(s) 316
BseRI GAGGAG 1 cut(s) 25
Bsh1236I CGCG 4 cut(s) 45, 101, 107, 121
BshFI GGCC 1 cut(s) 71
BshNI GGYRCC 1 cut(s) 20
BshVI ATCGAT 1 cut(s) 162
BsiHKAI GWGCWC 2 cut(s) 17, 309
BsiSI CCGG 4 cut(s) 18, 68, 129, 264
BslI CCNNNNNNNGG 1 cut(s) 219
BsmAI GTCTC 1 cut(s) 261
BsnI GGCC 1 cut(s) 71
Bsp1286I GDGCHC 2 cut(s) 17, 309
Bsp13I TCCGGA 1 cut(s) 263
Bsp143I GATC 2 cut(s) 168, 224
Bsp68I TCGCGA 1 cut(s) 121
BspACI CCGC 1 cut(s) 64
BspANI GGCC 1 cut(s) 71
BspDI ATCGAT 1 cut(s) 162
BspEI TCCGGA 1 cut(s) 263
BspFNI CGCG 4 cut(s) 45, 101, 107, 121
BspLI GGNNCC 1 cut(s) 22
BspMI ACCTGC 1 cut(s) 152
BspPI GGATC 1 cut(s) 219
BspT107I GGYRCC 1 cut(s) 20
BspTI CTTAAG 1 cut(s) 293
BsrI ACTGG 1 cut(s) 316
BssECI CCNNGG 3 cut(s) 218, 228, 323
BssMI GATC 2 cut(s) 168, 224
BssNI GRCGYC 1 cut(s) 21
BssT1I CCWWGG 3 cut(s) 218, 228, 323
Bst6I CTCTTC 1 cut(s) 196
BstACI GRCGYC 1 cut(s) 21
BstAFI CTTAAG 1 cut(s) 293
BstC8I GCNNGC 4 cut(s) 52, 105, 123, 214
BstF5I GGATG 2 cut(s) 42, 150
BstFNI CGCG 4 cut(s) 45, 101, 107, 121
BstH2I RGCGCY 1 cut(s) 24
BstHHI GCGC 3 cut(s) 23, 45, 103
BstKTI GATC 2 cut(s) 171, 227
BstMAI GTCTC 1 cut(s) 261
BstMBI GATC 2 cut(s) 168, 224
BstMWI GCNNNNNNNGC 2 cut(s) 29, 51
BstSCI CCNGG 1 cut(s) 66
BstUI CGCG 4 cut(s) 45, 101, 107, 121
BstX2I RGATCY 1 cut(s) 224
BstYI RGATCY 1 cut(s) 224
Bsu15I ATCGAT 1 cut(s) 162
BsuI GTATCC 1 cut(s) 256
BsuRI GGCC 1 cut(s) 71
BsuTUI ATCGAT 1 cut(s) 162
BtgZI GCGATG 1 cut(s) 13
BtsCI GGATG 2 cut(s) 42, 150
BtsIMutI CAGTG 1 cut(s) 309
BtuMI TCGCGA 1 cut(s) 121
BveI ACCTGC 1 cut(s) 152
Cac8I GCNNGC 4 cut(s) 52, 105, 123, 214
CaiI CAGNNNCTG 1 cut(s) 358
CfoI GCGC 3 cut(s) 23, 45, 103
ClaI ATCGAT 1 cut(s) 162
CseI GACGC 1 cut(s) 85
Csp6I GTAC 1 cut(s) 355
CviAII CATG 2 cut(s) 76, 238
CviJI RGCY 9 cut(s) 15, 50, 71, 125, 187, 216, 292, 307, 328
CviKI_1 RGCY 9 cut(s) 15, 50, 71, 125, 187, 216, 292, 307, 328
CviQI GTAC 1 cut(s) 355
DinI GGCGCC 1 cut(s) 22
DpnI GATC 2 cut(s) 170, 226
DpnII GATC 2 cut(s) 168, 224
EaeI YGGCCR 1 cut(s) 69
Eam1104I CTCTTC 1 cut(s) 196
EarI CTCTTC 1 cut(s) 196
Ecl136II GAGCTC 2 cut(s) 15, 307
Eco130I CCWWGG 3 cut(s) 218, 228, 323
Eco24I GRGCYC 2 cut(s) 17, 309
Eco53kI GAGCTC 2 cut(s) 15, 307
EcoICRI GAGCTC 2 cut(s) 15, 307
EcoT14I CCWWGG 3 cut(s) 218, 228, 323
EcoT38I GRGCYC 2 cut(s) 17, 309
EgeI GGCGCC 1 cut(s) 22
EheI GGCGCC 1 cut(s) 22
ErhI CCWWGG 3 cut(s) 218, 228, 323
FaeI CATG 2 cut(s) 79, 241
FaiI YATR 6 cut(s) 77, 94, 134, 167, 239, 272
FatI CATG 2 cut(s) 75, 237
FauI CCCGC 1 cut(s) 71
FbaI TGATCA 1 cut(s) 168
FokI GGATG 2 cut(s) 49, 137
FriOI GRGCYC 2 cut(s) 17, 309
GlaI GCGC 3 cut(s) 22, 44, 102
HaeII RGCGCY 1 cut(s) 24
HaeIII GGCC 1 cut(s) 71
HapII CCGG 4 cut(s) 18, 68, 129, 264
HgaI GACGC 1 cut(s) 85
HhaI GCGC 3 cut(s) 23, 45, 103
Hin1I GRCGYC 1 cut(s) 21
Hin1II CATG 2 cut(s) 79, 241
Hin6I GCGC 3 cut(s) 21, 43, 101
HinP1I GCGC 3 cut(s) 21, 43, 101
HindIII AAGCTT 1 cut(s) 290
HinfI GANTC 2 cut(s) 191, 233
HpaII CCGG 4 cut(s) 18, 68, 129, 264
HphI GGTGA 1 cut(s) 326
Hpy166II GTNNAC 1 cut(s) 138
Hpy188I TCNGA 1 cut(s) 250
Hpy188III TCNNGA 2 cut(s) 120, 264
Hpy8I GTNNAC 1 cut(s) 138
Hpy99I CGWCG 1 cut(s) 101
HpyAV CCTTC 2 cut(s) 34, 286
HpyCH4V TGCA 2 cut(s) 147, 352
HpyF10VI GCNNNNNNNGC 2 cut(s) 29, 51
Hsp92I GRCGYC 1 cut(s) 21
Hsp92II CATG 2 cut(s) 79, 241
HspAI GCGC 3 cut(s) 21, 43, 101
KasI GGCGCC 1 cut(s) 20
Kpn2I TCCGGA 1 cut(s) 263
Ksp22I TGATCA 1 cut(s) 168
Kzo9I GATC 2 cut(s) 168, 224
LmnI GCTCC 2 cut(s) 12, 20
LpnPI CCDG 8 cut(s) 31, 81, 142, 157, 197, 226, 277, 297
LweI GCATC 1 cut(s) 156
MalI GATC 2 cut(s) 170, 226
MboI GATC 2 cut(s) 168, 224
MboII GAAGA 1 cut(s) 213
MflI RGATCY 1 cut(s) 224
MhlI GDGCHC 2 cut(s) 17, 309
MluCI AATT 1 cut(s) 176
Mly113I GGCGCC 1 cut(s) 21
MmeI TCCRAC 2 cut(s) 178, 273
MnlI CCTC 2 cut(s) 3, 136
MroI TCCGGA 1 cut(s) 263
MseI TTAA 1 cut(s) 294
MslI CAYNNNNRTG 1 cut(s) 164
MspCI CTTAAG 1 cut(s) 293
MspI CCGG 4 cut(s) 18, 68, 129, 264
MspR9I CCNGG 1 cut(s) 68
MvnI CGCG 4 cut(s) 45, 101, 107, 121
MwoI GCNNNNNNNGC 2 cut(s) 29, 51
NarI GGCGCC 1 cut(s) 21
NciI CCSGG 1 cut(s) 68
NdeII GATC 2 cut(s) 168, 224
NlaIII CATG 2 cut(s) 79, 241
NlaIV GGNNCC 1 cut(s) 22
NruI TCGCGA 1 cut(s) 121
PfeI GAWTC 2 cut(s) 191, 233
PflMI CCANNNNNTGG 1 cut(s) 219
PluTI GGCGCC 1 cut(s) 24
Psp124BI GAGCTC 2 cut(s) 17, 309
PspN4I GGNNCC 1 cut(s) 22
PstNI CAGNNNCTG 1 cut(s) 358
PsuI RGATCY 1 cut(s) 224
RruI TCGCGA 1 cut(s) 121
RsaI GTAC 1 cut(s) 356
RsaNI GTAC 1 cut(s) 355
RseI CAYNNNNRTG 1 cut(s) 164
SacI GAGCTC 2 cut(s) 17, 309
SaqAI TTAA 1 cut(s) 294
Sau3AI GATC 2 cut(s) 168, 224
ScrFI CCNGG 1 cut(s) 68
SduI GDGCHC 2 cut(s) 17, 309
SetI ASST 7 cut(s) 17, 146, 189, 294, 303, 309, 360
SfaNI GCATC 1 cut(s) 156
SfoI GGCGCC 1 cut(s) 22
SmiMI CAYNNNNRTG 1 cut(s) 164
SmlI CTYRAG 2 cut(s) 293, 302
SmoI CTYRAG 2 cut(s) 293, 302
Sse9I AATT 1 cut(s) 176
SsiI CCGC 1 cut(s) 64
SspDI GGCGCC 1 cut(s) 20
SstI GAGCTC 2 cut(s) 17, 309
StyD4I CCNGG 1 cut(s) 66
StyI CCWWGG 3 cut(s) 218, 228, 323
TaqI TCGA 1 cut(s) 162
TasI AATT 1 cut(s) 176
TfiI GAWTC 2 cut(s) 191, 233
Tru1I TTAA 1 cut(s) 294
Tru9I TTAA 1 cut(s) 294
TscAI CASTG 1 cut(s) 316
TspDTI ATGAA 3 cut(s) 16, 254, 357
TspGWI ACGGA 2 cut(s) 49, 249
TspRI CASTG 1 cut(s) 316
Van91I CCANNNNNTGG 1 cut(s) 219
Vha464I CTTAAG 1 cut(s) 293
XapI RAATTY 1 cut(s) 176
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.