Rroxscaffold_7G00188050

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0013736
Physical Location & Seq
Reverse (-)
27529118 .. 27530916
1799 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00188050.1

Sequence Viewer

Length: 477 bp
ATGCGTCGCGCACGCGATTGGGAACTTTGCCGAGCCTCCGTATGCTCGCTACCTCGCATCATCCAACCCATCTATATGATCAAGATCTATGATTTGTTTGGCTCCGTTGATGGAGGAGAGGGGGCTTTGAAATGGAGGAAGAACAAGGATGTTGTTCGTGGTTTGGGTTGTCTCTCGTCTCGCTTTTTCCTCTTGCTTCGTCACCGAACGGTGAAGGAATTTGTCCAGCTTGAATCTTGGCGAAGAGATTTTGCCAGCCCTTGGAGATCCGGGAAACTTGGAATCCATGAATTATCCGACAATAATCCTTCCGGAGACATACCCTTCTCGGTTTCAAAGCTTAAGAACCTTGAGGTTTTGAATTTGAAGAACAATCGCTTGACTGGTCCTATCGCTACAACTCTTACCCAAATTCCAAACCTTAGAACTTTGCAACTCTTTGATGAAGCACTGTCCCTCGATGTGTCAATTAACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

158

Amino Acids

18.07

Weight (kDa)

9.81

Isoelectric Point (pI)

35.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_8 PF13855 98 - 148 3.5e-06 Leucine rich repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 261
AccII CGCG 2 cut(s) 9, 15
AccIII TCCGGA 1 cut(s) 311
AclWI GGATC 1 cut(s) 261
AcsI RAATTY 3 cut(s) 218, 361, 411
AfiI CCNNNNNNNGG 1 cut(s) 261
AflII CTTAAG 1 cut(s) 341
AgsI TTSAA 5 cut(s) 130, 233, 336, 361, 367
AluBI AGCT 2 cut(s) 229, 340
AluI AGCT 2 cut(s) 229, 340
Alw26I GTCTC 3 cut(s) 176, 183, 309
AlwI GGATC 1 cut(s) 261
Aor13HI TCCGGA 1 cut(s) 311
ApoI RAATTY 3 cut(s) 218, 361, 411
AspLEI GCGC 1 cut(s) 11
AspS9I GGNCC 1 cut(s) 386
AsuC2I CCSGG 1 cut(s) 271
AsuHPI GGTGA 2 cut(s) 194, 223
AvaII GGWCC 1 cut(s) 386
BccI CCATC 2 cut(s) 77, 104
BclI TGATCA 1 cut(s) 78
BcnI CCSGG 1 cut(s) 271
BcoDI GTCTC 3 cut(s) 176, 183, 309
BfaI CTAG 1 cut(s) 475
BfrI CTTAAG 1 cut(s) 341
BglII AGATCT 1 cut(s) 84
Bme1390I CCNGG 1 cut(s) 271
Bme18I GGWCC 1 cut(s) 386
BmgT120I GGNCC 1 cut(s) 386
BmiI GGNNCC 1 cut(s) 103
BmrFI CCNGG 1 cut(s) 271
BmsI GCATC 1 cut(s) 66
BpuEI CTTGAG 1 cut(s) 371
BpuMI CCSGG 1 cut(s) 271
BsaBI GATNNNNATC 1 cut(s) 83
BsaJI CCNNGG 1 cut(s) 260
BsaWI WCCGGW 1 cut(s) 311
Bsc4I CCNNNNNNNGG 1 cut(s) 261
Bse1I ACTGG 1 cut(s) 388
Bse8I GATNNNNATC 1 cut(s) 83
BseAI TCCGGA 1 cut(s) 311
BseDI CCNNGG 1 cut(s) 260
BseGI GGATG 2 cut(s) 60, 154
BseJI GATNNNNATC 1 cut(s) 83
BseLI CCNNNNNNNGG 1 cut(s) 261
BseNI ACTGG 1 cut(s) 388
BseRI GAGGAG 1 cut(s) 129
Bsh1236I CGCG 2 cut(s) 9, 15
BsiSI CCGG 2 cut(s) 270, 312
BslFI GGGAC 1 cut(s) 439
BslI CCNNNNNNNGG 1 cut(s) 261
BsmAI GTCTC 3 cut(s) 176, 183, 309
BsmBI CGTCTC 1 cut(s) 183
BsmFI GGGAC 1 cut(s) 439
Bsp13I TCCGGA 1 cut(s) 311
Bsp143I GATC 3 cut(s) 78, 84, 266
BspEI TCCGGA 1 cut(s) 311
BspFNI CGCG 2 cut(s) 9, 15
BspLI GGNNCC 1 cut(s) 103
BspPI GGATC 1 cut(s) 261
BspTI CTTAAG 1 cut(s) 341
BsrI ACTGG 1 cut(s) 388
BssECI CCNNGG 1 cut(s) 260
BssMI GATC 3 cut(s) 78, 84, 266
BssT1I CCWWGG 1 cut(s) 260
Bst4CI ACNGT 2 cut(s) 211, 453
Bst6I CTCTTC 1 cut(s) 238
BstAFI CTTAAG 1 cut(s) 341
BstC8I GCNNGC 3 cut(s) 13, 47, 256
BstDEI CTNAG 1 cut(s) 422
BstF5I GGATG 2 cut(s) 60, 154
BstFNI CGCG 2 cut(s) 9, 15
BstHHI GCGC 1 cut(s) 11
BstKTI GATC 3 cut(s) 81, 87, 269
BstMAI GTCTC 3 cut(s) 176, 183, 309
BstMBI GATC 3 cut(s) 78, 84, 266
BstSCI CCNGG 1 cut(s) 269
BstUI CGCG 2 cut(s) 9, 15
BstX2I RGATCY 2 cut(s) 84, 266
BstYI RGATCY 2 cut(s) 84, 266
BtsCI GGATG 2 cut(s) 60, 154
BtsIMutI CAGTG 1 cut(s) 449
Cac8I GCNNGC 3 cut(s) 13, 47, 256
CfoI GCGC 1 cut(s) 11
Cfr13I GGNCC 1 cut(s) 386
CviAII CATG 1 cut(s) 287
CviJI RGCY 6 cut(s) 35, 102, 125, 229, 258, 340
CviKI_1 RGCY 6 cut(s) 35, 102, 125, 229, 258, 340
DdeI CTNAG 1 cut(s) 422
DpnI GATC 3 cut(s) 80, 86, 268
DpnII GATC 3 cut(s) 78, 84, 266
Eam1104I CTCTTC 1 cut(s) 238
EarI CTCTTC 1 cut(s) 238
Eco130I CCWWGG 1 cut(s) 260
Eco47I GGWCC 1 cut(s) 386
EcoT14I CCWWGG 1 cut(s) 260
ErhI CCWWGG 1 cut(s) 260
Esp3I CGTCTC 1 cut(s) 183
FaeI CATG 1 cut(s) 290
FaiI YATR 6 cut(s) 43, 75, 77, 90, 288, 320
FaqI GGGAC 1 cut(s) 439
FatI CATG 1 cut(s) 286
FbaI TGATCA 1 cut(s) 78
FokI GGATG 2 cut(s) 47, 161
FspBI CTAG 1 cut(s) 475
GlaI GCGC 1 cut(s) 10
HapII CCGG 2 cut(s) 270, 312
HhaI GCGC 1 cut(s) 11
Hin1II CATG 1 cut(s) 290
Hin6I GCGC 1 cut(s) 9
HinP1I GCGC 1 cut(s) 9
HindIII AAGCTT 1 cut(s) 338
HinfI GANTC 2 cut(s) 233, 282
HpaII CCGG 2 cut(s) 270, 312
HphI GGTGA 2 cut(s) 194, 223
Hpy188I TCNGA 1 cut(s) 298
Hpy188III TCNNGA 2 cut(s) 82, 312
Hpy99I CGWCG 1 cut(s) 9
HpyAV CCTTC 3 cut(s) 208, 318, 334
HpyCH4III ACNGT 2 cut(s) 211, 453
HpyCH4V TGCA 1 cut(s) 433
HpyF3I CTNAG 1 cut(s) 422
Hsp92II CATG 1 cut(s) 290
HspAI GCGC 1 cut(s) 9
Kpn2I TCCGGA 1 cut(s) 311
Ksp22I TGATCA 1 cut(s) 78
Kzo9I GATC 3 cut(s) 78, 84, 266
LmnI GCTCC 1 cut(s) 107
LpnPI CCDG 5 cut(s) 239, 268, 283, 325, 369
LweI GCATC 1 cut(s) 66
MaeI CTAG 1 cut(s) 475
MaeIII GTNAC 1 cut(s) 200
MalI GATC 3 cut(s) 80, 86, 268
MboI GATC 3 cut(s) 78, 84, 266
MboII GAAGA 3 cut(s) 151, 255, 379
MflI RGATCY 2 cut(s) 84, 266
MluCI AATT 5 cut(s) 218, 290, 361, 411, 468
MmeI TCCRAC 2 cut(s) 88, 321
MnlI CCTC 8 cut(s) 46, 63, 107, 112, 129, 200, 346, 467
MroI TCCGGA 1 cut(s) 311
MseI TTAA 2 cut(s) 342, 471
MslI CAYNNNNRTG 1 cut(s) 74
MspCI CTTAAG 1 cut(s) 341
MspI CCGG 2 cut(s) 270, 312
MspR9I CCNGG 1 cut(s) 271
MvnI CGCG 2 cut(s) 9, 15
NciI CCSGG 1 cut(s) 271
NdeII GATC 3 cut(s) 78, 84, 266
NlaIII CATG 1 cut(s) 290
NlaIV GGNNCC 1 cut(s) 103
NmeAIII GCCGAG 1 cut(s) 56
NmuCI GTSAC 1 cut(s) 200
PfeI GAWTC 2 cut(s) 233, 282
PflMI CCANNNNNTGG 1 cut(s) 261
PfoI TCCNGGA 1 cut(s) 269
PspN4I GGNNCC 1 cut(s) 103
PspPI GGNCC 1 cut(s) 386
PsuI RGATCY 2 cut(s) 84, 266
RseI CAYNNNNRTG 1 cut(s) 74
SaqAI TTAA 2 cut(s) 342, 471
Sau3AI GATC 3 cut(s) 78, 84, 266
Sau96I GGNCC 1 cut(s) 386
ScrFI CCNGG 1 cut(s) 271
SetI ASST 6 cut(s) 55, 231, 342, 351, 357, 423
SfaNI GCATC 1 cut(s) 66
SinI GGWCC 1 cut(s) 386
SmiMI CAYNNNNRTG 1 cut(s) 74
SmlI CTYRAG 2 cut(s) 341, 350
SmoI CTYRAG 2 cut(s) 341, 350
Sse9I AATT 5 cut(s) 218, 290, 361, 411, 468
SspMI CTAG 1 cut(s) 475
StyD4I CCNGG 1 cut(s) 269
StyI CCWWGG 1 cut(s) 260
TaaI ACNGT 2 cut(s) 211, 453
TaqI TCGA 1 cut(s) 459
TasI AATT 5 cut(s) 218, 290, 361, 411, 468
TfiI GAWTC 2 cut(s) 233, 282
Tru1I TTAA 2 cut(s) 342, 471
Tru9I TTAA 2 cut(s) 342, 471
TscAI CASTG 1 cut(s) 456
TseFI GTSAC 1 cut(s) 200
Tsp45I GTSAC 1 cut(s) 200
TspDTI ATGAA 2 cut(s) 303, 459
TspGWI ACGGA 2 cut(s) 28, 94
TspRI CASTG 1 cut(s) 456
Van91I CCANNNNNTGG 1 cut(s) 261
Vha464I CTTAAG 1 cut(s) 341
VpaK11BI GGWCC 1 cut(s) 386
XapI RAATTY 3 cut(s) 218, 361, 411
XspI CTAG 1 cut(s) 475
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.