Basic Information

Type: gene

Biological Identity

Species

rosa_multiflora

Chromosome

Rmu_sc0005970.1

Orthogroup ID

OG0002217

Physical Location & Seq

Genomic Coordinates Reverse (-)

39520 .. 44809

Gene Structure 5290 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3309 bp

atggtcaaattagggttaaagttttgggggtgggttgctgtagcttcggcgccgatctttgtcggattggcgttccgaagctactccggcgtcggcgacggtgccgattcggttgattttccggttttgtgtttggttgtttcggtgatcgtcgggttttgtggcgtgttcagcagcttactgcactatatgcttcctagaaagagaccttgtgaaggagaagaaggagacggtgacgttaacaagaccactggtaccagctcggcggcttctgcgtctttgatcaagaagcttcggatcgaatcctacgttgccgaatcggccatcaacaacagcagtaacaacagcagcagcaacggtagcggtagcgtggttgagaacgacgtgccgatcatggctttgggagattccaacgccggagatatcgatgaggatctgcacagccggcagcttgccgtctatggccgggagacgatgaggaggctctttgcatccaatgttctgatctcaggggtacagggcctcggtgctgagattgccaaaaatctcattcttgctggtgtgaagtctgtgaccttgcatgatgaagggactgtggagctttgggatttgtccagcaattttatttttacagaggatgatgttggtaagaacagggcacttgcttctgttcaaaagttgcaggaactcaacaatgctgtgattgttcataccttgacaactccattgactacggagcagctttctgatttccaggctgttgtattcactgatatcaattacgagaaagctattgaattcaatgattattgtcataatcatcagccgccaatcgccttcataagaaccgaagttagaggcctttttggttctgtattttgtgactttggacctgaattcactgtttttgatgttgatggggaggaaccacacacgggtattattgcatcaatcagcaatgacaaccctgctcttgtgtcatgtgtcgatgatgaaagacttgaatttcaggatggggatcttgttgtgttctcagaagttcatggaatgacagaactaaatgatggaaagccaaggaagataaaaaatgctagggcttactcatttacgcttgaggaggatacctcaggttttggtacctatgtgaaaggtggcattgtcacacaagtgaagcagccaaaagtgttgaacttcaagccattgggggaagcactcaagaatcccggtgattttcttcttagtgatttctccaagtttgatcgccctcctctcctacacttggcatttcaagcactggacaaatttgtttctgagttgggacgataccctgttgctggttcagaagaggatgctcagaagcttatatctgttgctactaacattaataataaactgggagatggcaggctggaagatcttaatccaaaacttttgcgtcacttcgcctttggtgcaaaggcggtactaaatcccatggctgccatgtttggtggtcttgttggtcaagaggttgtaaaagcttgctctggaaagtttcatccactcttccagttcttctactttgactcggtagaatcacttccaacggagccactggattccagtgatttgaaaccattaaatagccgttatgatgcacagatttcagtttttgggtccaagctacagaagaaactggaggatgccaaagtattcatggttggagctggtgcacttggatgtgagttgttgaaaaatgttgccctgatgggagtttcatgtggcgaccaagggaagctaacaatcactgatgatgatgtaattgagaagagtaacctcagtaggcagttccttttccgtgattggaacattgggcaggcaaagtctacagttgccgcttctgctgctgcatcaataaatccccgtctcaacattgtagcgttgcagaatagagttggtcctgaaactgagaatgtgtttgatgatacgtactgggagaatttaagtgttgttattaatgcactagataatgtcaatgccaggctctatgttgatcagcggtgcttatatttccagaaggcacttcttgagtcgggtactctgggggcgaaatgcaacactcagatggttattcctcacctaacagaaaactatggtgcctctagagatcctccagagaaacaagcacccattaatgctggggatgcacaggccagggacaccttggagcgtgttcttgagtgcctttccagagaaagatgcgaaacattccaagattgtattgcctgggctcgcctcaagtttgaagattatttttccaaccgggtgaaacagctgacatatactttccctgaagatgctgcaactagtactggggctccattctggtctgcccccaagcgattcccccgtgcactgcagttctcagctaatgatcccggtcatctccattttgtcatggcagcatccatactcagggcagagacatttggtatcccaatccctgactgggttaggaactctaagaagttgtctgaagctgttgagaaagtggaagtcccagactttcagcccaagaaagatgcaaaaatagtgacagatgataaggccaccaatttaactcctcaatctatagatgatgcccaggttattaatgaattgattatcaagttagagcattgtcgagagaaactcccaccagggttccggatgaaaccgattcaattcgagaaggatgatgataccaattaccatatggatatgatagctgggcttgccaatatgagggctaggaattacagcattcctgaggtggacaagctgaaagccaagtttattgccggtaggattatccctgcaattgcaacctccactgcaatggccacaggacttgtgtgcctggaactttacaaggttctggatggcgaccacaaactggaagactacagaaatacatttgcaaacctagcactacctctgttctccatggctgagccggtacctcccaaggtcattaagcacaaggacatgagctggacagtgtgggataggtggatcctgaggggcaatcctactctgagggaacttctccagtggcttaaggataagggactgaatgcttatagcatctcttgtggaagctctctgctttttaacagcatgtttgctcgacacaaagaccgaatggacaagaagttggtggatctggctaaggacgtggcgaaggtggaaattcccccatatcgttgccacttggatgttgttgttgcttgtgaggatgatgaggacaatgacattgacatcccattggtgtcggtttacttccgttag

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004842 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0005886 GO:0005911 GO:0006464 GO:0006807 GO:0006950 GO:0006974 GO:0008150 GO:0008152 GO:0009506 GO:0009605 GO:0009607 GO:0009987 GO:0010035 GO:0010038 GO:0016020 GO:0016567 GO:0016740 GO:0019538 GO:0019787 GO:0030054 GO:0032446 GO:0033554 GO:0036211 GO:0042221 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0046686 GO:0050896 GO:0051704 GO:0051707 GO:0051716 GO:0055044 GO:0070647 GO:0071704 GO:0071944 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

ko04120 ko05012 map04120 map05012

Pfam Domains

Protein Families

E1_4HB E1_FCCH E1_UFD ThiF UBA_e1_thiolCys

Protein Analysis

1102

Amino Acids

121.89

Weight (kDa)

5.24

Isoelectric Point (pI)

31.4

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
ThiF	PF00899	147 - 522	1e-27	ThiF family
E1_FCCH	PF16190	321 - 391	1.4e-28	Ubiquitin-activating enzyme E1 FCCH domain
E1_4HB	PF16191	392 - 462	1.4e-25	Ubiquitin-activating enzyme E1 four-helix bundle
ThiF	PF00899	544 - 989	5.3e-56	ThiF family
UBA_E1_SCCH	PF10585	725 - 937	7.4e-71	Ubiquitin-activating enzyme, SCCH domain
E1_UFD	PF09358	1008 - 1097	3e-23	Ubiquitin fold domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0002217)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G30110 AT5G06460
fragaria_vesca	FvH4_3g39370 FvH4_3g39370 FvH4_3g39370 FvH4_3g39370 FvH4_3g39370 FvH4_3g39370 FvH4_3g39370 FvH4_3g39370 FvH4_3g39370 FvH4_6g10930 FvH4_6g10930 FvH4_6g10930
malus_domestica	MD04G1163500.v1.1 MD11G1065700.v1.1 MD12G1177300.v1.1
prunus_persica	Prupe.6G051900_v2.0.a1 Prupe.6G051900_v2.0.a1 Prupe.6G051900_v2.0.a1 Prupe.6G051900_v2.0.a1 Prupe.6G282900_v2.0.a1
pyrus_communis	pycom04g14620 pycom11g05570 pycom12g16790
rosa_chinensis	RchiOBHm_Chr3g0462201 RchiOBHm_Chr5g0070911
rosa_laevigata	RLG00000024878 RLG00000036170
rosa_multiflora	Rmu_sc0002944.1_g000004 Rmu_sc0005970.1_g000009 Rmu_sc0006715.1_g000003
rosa_roxburghii	Rroxscaffold_1G00010290 Rroxscaffold_6G00417690
rosa_rugosa	Rorug03G0054900 Rorug05G0408500 Rorug05G0408500 Rorug05G0408500
rosa_samantha	Rh3CG119500 Rh5AG464600 Rh5DG494900
rosa_wichuraiana	Rw3G009590 Rw5G043220

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc65I	GGTACC	3 cut(s)	254, 1136, 2977
AccB1I	GGYRCC	6 cut(s)	49, 101, 254, 1136, 2135, 2977
AccB7I	CCANNNNNTGG	1 cut(s)	2914
AccI	GTMKAC	1 cut(s)	1865
AccIII	TCCGGA	1 cut(s)	2685
AciI	CCGC	6 cut(s)	266, 363, 827, 1460, 1875, 2038
AclWI	GGATC	8 cut(s)	305, 441, 1026, 2141, 2408, 3028, 3041, 3189
AcoI	YGGCCR	3 cut(s)	321, 463, 2859
AcsI	RAATTY	6 cut(s)	797, 896, 1004, 1301, 1978, 3210
AcuI	CTGAAG	2 cut(s)	2352, 2535
AcyI	GRCGYC	2 cut(s)	50, 90
AfaI	GTAC	8 cut(s)	256, 516, 1138, 1464, 1970, 2077, 2350, 2979
AfiI	CCNNNNNNNGG	8 cut(s)	215, 935, 1279, 1334, 2454, 2488, 2763, 2914
AflII	CTTAAG	1 cut(s)	3077
AhlI	ACTAGT	1 cut(s)	2345
AjiI	CACGTC	2 cut(s)	385, 3196
AjnI	CCWGG	7 cut(s)	753, 2018, 2192, 2264, 2622, 2677, 2877
AleI	CACNNNNGTG	1 cut(s)	1166
Alw21I	GWGCWC	2 cut(s)	1714, 2395
Alw26I	GTCTC	5 cut(s)	199, 222, 464, 1910, 2456
Alw44I	GTGCAC	2 cut(s)	1710, 2391
AlwI	GGATC	8 cut(s)	305, 441, 1026, 2141, 2408, 3028, 3041, 3189
Aor13HI	TCCGGA	1 cut(s)	2685
AoxI	GGCC	7 cut(s)	321, 463, 520, 859, 2190, 2586, 2859
ApaLI	GTGCAC	2 cut(s)	1710, 2391
ApoI	RAATTY	6 cut(s)	797, 896, 1004, 1301, 1978, 3210
AseI	ATTAAT	4 cut(s)	1383, 1995, 2172, 2631
Asp700I	GAANNNNTTC	1 cut(s)	1578
Asp718I	GGTACC	3 cut(s)	254, 1136, 2977
AspLEI	GCGC	1 cut(s)	52
AspS9I	GGNCC	4 cut(s)	520, 890, 1656, 1937
AsuC2I	CCSGG	4 cut(s)	467, 1224, 2303, 2418
AsuHPI	GGTGA	5 cut(s)	157, 245, 1238, 2108, 2317
AvaII	GGWCC	3 cut(s)	890, 1656, 1937
AxyI	CCTNAGG	3 cut(s)	1126, 2787, 3038
BaeGI	GKGCMC	3 cut(s)	661, 1714, 2395
BaeI	ACNNNNGTAYC	2 cut(s)	1128, 1161
BalI	TGGCCA	1 cut(s)	2861
BamHI	GGATCC	1 cut(s)	3033
BanI	GGYRCC	6 cut(s)	49, 101, 254, 1136, 2135, 2977
BanII	GRGCYC	2 cut(s)	2272, 2359
BarI	GAAGNNNNNNTAC	2 cut(s)	1563, 1595
BbsI	GAAGAC	1 cut(s)	2925
Bbv12I	GWGCWC	2 cut(s)	1714, 2395
BccI	CCATC	8 cut(s)	332, 911, 1007, 1058, 1394, 1741, 2098, 2894
BceAI	ACGGC	2 cut(s)	440, 1611
BciT130I	CCWGG	7 cut(s)	755, 2020, 2194, 2266, 2624, 2679, 2879
BciVI	GTATCC	2 cut(s)	1114, 2483
BclI	TGATCA	2 cut(s)	282, 2032
BcnI	CCSGG	4 cut(s)	467, 1224, 2303, 2418
BcoDI	GTCTC	5 cut(s)	199, 222, 464, 1910, 2456
BcuI	ACTAGT	1 cut(s)	2345
BfaI	CTAG	7 cut(s)	198, 1092, 2003, 2142, 2346, 2769, 2945
BfmI	CTRYAG	6 cut(s)	39, 1664, 1866, 2396, 2610, 2923
BfoI	RGCGCY	1 cut(s)	53
BfrI	CTTAAG	1 cut(s)	3077
BfuI	GTATCC	2 cut(s)	1114, 2483
BglI	GCCNNNNNGGC	1 cut(s)	320
BglII	AGATCT	1 cut(s)	1414
BlpI	GCTNAGC	1 cut(s)	2970
BmcAI	AGTACT	1 cut(s)	2350
Bme18I	GGWCC	3 cut(s)	890, 1656, 1937
BmgBI	CACGTC	2 cut(s)	385, 3196
BmgT120I	GGNCC	4 cut(s)	520, 890, 1656, 1937
BmrI	ACTGGG	4 cut(s)	1403, 1981, 2361, 2497
BmuI	ACTGGG	4 cut(s)	1403, 1981, 2361, 2497
BpiI	GAAGAC	1 cut(s)	2925
BplI	GAGNNNNNCTC	4 cut(s)	1109, 1141, 2655, 2687
BpmI	CTGGAG	3 cut(s)	1697, 2136, 3053
Bpu10I	CCTNAGC	1 cut(s)	3189
Bpu1102I	GCTNAGC	1 cut(s)	2970
BpuEI	CTTGAG	5 cut(s)	1133, 1199, 2087, 2237, 2261
BpuMI	CCSGG	4 cut(s)	467, 1224, 2303, 2418
Bsa29I	ATCGAT	1 cut(s)	426
BsaAI	YACGTR	1 cut(s)	1968
BsaBI	GATNNNNATC	3 cut(s)	432, 1017, 1419
BsaHI	GRCGYC	2 cut(s)	50, 90
BsaI	GGTCTC	1 cut(s)	199
BsaWI	WCCGGW	2 cut(s)	121, 2685
BsaXI	ACNNNNNCTCC	2 cut(s)	2341, 2371
Bsc4I	CCNNNNNNNGG	8 cut(s)	215, 935, 1279, 1334, 2454, 2488, 2763, 2914
Bse118I	RCCGGY	3 cut(s)	444, 2819, 2974
Bse21I	CCTNAGG	3 cut(s)	1126, 2787, 3038
Bse3DI	GCAATG	2 cut(s)	964, 2862
Bse8I	GATNNNNATC	3 cut(s)	432, 1017, 1419
BseAI	TCCGGA	1 cut(s)	2685
BseBI	CCWGG	7 cut(s)	755, 2020, 2194, 2266, 2624, 2679, 2879
BseCI	ATCGAT	1 cut(s)	426
BseJI	GATNNNNATC	3 cut(s)	432, 1017, 1419
BseLI	CCNNNNNNNGG	8 cut(s)	215, 935, 1279, 1334, 2454, 2488, 2763, 2914
BseMI	GCAATG	2 cut(s)	964, 2862
BseRI	GAGGAG	4 cut(s)	493, 1130, 1257, 2592
BseSI	GKGCMC	3 cut(s)	661, 1714, 2395
BseYI	CCCAGC	2 cut(s)	2177, 2747
BsgI	GTGCAG	2 cut(s)	167, 422
BshFI	GGCC	7 cut(s)	323, 465, 522, 861, 2192, 2588, 2861
BshNI	GGYRCC	6 cut(s)	49, 101, 254, 1136, 2135, 2977
BshVI	ATCGAT	1 cut(s)	426
BsiHKAI	GWGCWC	2 cut(s)	1714, 2395
BslFI	GGGAC	5 cut(s)	604, 1332, 2210, 2522, 3102
BslI	CCNNNNNNNGG	8 cut(s)	215, 935, 1279, 1334, 2454, 2488, 2763, 2914
BsmAI	GTCTC	5 cut(s)	199, 222, 464, 1910, 2456
BsmBI	CGTCTC	3 cut(s)	222, 464, 1910
BsmFI	GGGAC	5 cut(s)	604, 1332, 2210, 2522, 3102
BsmI	GAATGC	2 cut(s)	2781, 3100
BsnI	GGCC	7 cut(s)	323, 465, 522, 861, 2192, 2588, 2861
Bso31I	GGTCTC	1 cut(s)	199
Bsp1286I	GDGCHC	5 cut(s)	661, 1714, 2272, 2359, 2395
Bsp13I	TCCGGA	1 cut(s)	2685
Bsp1720I	GCTNAGC	1 cut(s)	2970
Bsp19I	CCATGG	2 cut(s)	1473, 2964
BspACI	CCGC	6 cut(s)	266, 363, 827, 1460, 1875, 2038
BspANI	GGCC	7 cut(s)	323, 465, 522, 861, 2192, 2588, 2861
BspDI	ATCGAT	1 cut(s)	426
BspEI	TCCGGA	1 cut(s)	2685
BspMAI	CTGCAG	1 cut(s)	2400
BspPI	GGATC	8 cut(s)	305, 441, 1026, 2141, 2408, 3028, 3041, 3189
BspT107I	GGYRCC	6 cut(s)	49, 101, 254, 1136, 2135, 2977
BspTI	CTTAAG	1 cut(s)	3077
BspTNI	GGTCTC	1 cut(s)	199
BsrDI	GCAATG	2 cut(s)	964, 2862
BsrFI	RCCGGY	3 cut(s)	444, 2819, 2974
BssAI	RCCGGY	3 cut(s)	444, 2819, 2974
BssNI	GRCGYC	2 cut(s)	50, 90
BssT1I	CCWWGG	6 cut(s)	1073, 1473, 1768, 2202, 2964, 2985
Bst2UI	CCWGG	7 cut(s)	755, 2020, 2194, 2266, 2624, 2679, 2879
Bst4CI	ACNGT	7 cut(s)	101, 233, 359, 595, 904, 1870, 3019
Bst6I	CTCTTC	3 cut(s)	1338, 1550, 1802
BstACI	GRCGYC	2 cut(s)	50, 90
BstAFI	CTTAAG	1 cut(s)	3077
BstAPI	GCANNNNNTGC	1 cut(s)	190
BstBAI	YACGTR	1 cut(s)	1968
BstC8I	GCNNGC	7 cut(s)	446, 453, 1406, 1522, 1857, 2272, 2754
BstDSI	CCRYGG	2 cut(s)	1473, 2964
BstENI	CCTNNNNNAGG	1 cut(s)	213
BstH2I	RGCGCY	1 cut(s)	53
BstHHI	GCGC	1 cut(s)	52
BstMAI	GTCTC	5 cut(s)	199, 222, 464, 1910, 2456
BstNI	CCWGG	7 cut(s)	755, 2020, 2194, 2266, 2624, 2679, 2879
BstNSI	RCATGY	1 cut(s)	3142
BstSFI	CTRYAG	6 cut(s)	39, 1664, 1866, 2396, 2610, 2923
BstSLI	GKGCMC	3 cut(s)	661, 1714, 2395
BstSNI	TACGTA	1 cut(s)	1968
BstV2I	GAAGAC	1 cut(s)	2925
BstX2I	RGATCY	6 cut(s)	433, 1018, 1414, 2146, 3033, 3181
BstXI	CCANNNNNNTGG	1 cut(s)	2857
BstYI	RGATCY	6 cut(s)	433, 1018, 1414, 2146, 3033, 3181
Bsu15I	ATCGAT	1 cut(s)	426
Bsu36I	CCTNAGG	3 cut(s)	1126, 2787, 3038
BsuI	GTATCC	2 cut(s)	1114, 2483
BsuRI	GGCC	7 cut(s)	323, 465, 522, 861, 2192, 2588, 2861
BsuTUI	ATCGAT	1 cut(s)	426
BtgI	CCRYGG	2 cut(s)	1473, 2964
BtrI	CACGTC	2 cut(s)	385, 3196
BtsI	GCAGTG	2 cut(s)	2393, 2850
Cac8I	GCNNGC	7 cut(s)	446, 453, 1406, 1522, 1857, 2272, 2754
CfoI	GCGC	1 cut(s)	52
Cfr10I	RCCGGY	3 cut(s)	444, 2819, 2974
Cfr13I	GGNCC	4 cut(s)	520, 890, 1656, 1937
ClaI	ATCGAT	1 cut(s)	426
CseI	GACGC	3 cut(s)	79, 264, 1424
Csp6I	GTAC	8 cut(s)	255, 515, 1137, 1463, 1969, 2076, 2349, 2978
CspCI	CAANNNNNGTGG	2 cut(s)	2851, 2886
CviQI	GTAC	8 cut(s)	255, 515, 1137, 1463, 1969, 2076, 2349, 2978
DinI	GGCGCC	1 cut(s)	51
EaeI	YGGCCR	3 cut(s)	321, 463, 2859
Eam1104I	CTCTTC	3 cut(s)	1338, 1550, 1802
EarI	CTCTTC	3 cut(s)	1338, 1550, 1802
Eco105I	TACGTA	1 cut(s)	1968
Eco130I	CCWWGG	6 cut(s)	1073, 1473, 1768, 2202, 2964, 2985
Eco147I	AGGCCT	1 cut(s)	861
Eco24I	GRGCYC	2 cut(s)	2272, 2359
Eco31I	GGTCTC	1 cut(s)	199
Eco32I	GATATC	2 cut(s)	424, 775
Eco47I	GGWCC	3 cut(s)	890, 1656, 1937
Eco57I	CTGAAG	2 cut(s)	2352, 2535
Eco81I	CCTNAGG	3 cut(s)	1126, 2787, 3038
EcoNI	CCTNNNNNAGG	1 cut(s)	213
EcoO109I	RGGNCCY	1 cut(s)	520
EcoRI	GAATTC	2 cut(s)	797, 896
EcoRII	CCWGG	7 cut(s)	753, 2018, 2192, 2264, 2622, 2677, 2877
EcoRV	GATATC	2 cut(s)	424, 775
EcoT14I	CCWWGG	6 cut(s)	1073, 1473, 1768, 2202, 2964, 2985
EcoT38I	GRGCYC	2 cut(s)	2272, 2359
EgeI	GGCGCC	1 cut(s)	51
EheI	GGCGCC	1 cut(s)	51
ErhI	CCWWGG	6 cut(s)	1073, 1473, 1768, 2202, 2964, 2985
Esp3I	CGTCTC	3 cut(s)	222, 464, 1910
FaqI	GGGAC	5 cut(s)	604, 1332, 2210, 2522, 3102
FauNDI	CATATG	1 cut(s)	2733
FbaI	TGATCA	2 cut(s)	282, 2032
FblI	GTMKAC	1 cut(s)	1865
FriOI	GRGCYC	2 cut(s)	2272, 2359
FspBI	CTAG	7 cut(s)	198, 1092, 2003, 2142, 2346, 2769, 2945
GlaI	GCGC	1 cut(s)	51
GsaI	CCCAGC	2 cut(s)	2181, 2751
GsuI	CTGGAG	3 cut(s)	1697, 2136, 3053
HaeII	RGCGCY	1 cut(s)	53
HaeIII	GGCC	7 cut(s)	323, 465, 522, 861, 2192, 2588, 2861
HgaI	GACGC	3 cut(s)	79, 264, 1424
HhaI	GCGC	1 cut(s)	52
Hin1I	GRCGYC	2 cut(s)	50, 90
Hin6I	GCGC	1 cut(s)	50
HinP1I	GCGC	1 cut(s)	50
HincII	GTYRAC	1 cut(s)	241
HindII	GTYRAC	1 cut(s)	241
HindIII	AAGCTT	3 cut(s)	290, 1358, 1518
HpaI	GTTAAC	1 cut(s)	241
HphI	GGTGA	5 cut(s)	157, 245, 1238, 2108, 2317
Hpy166II	GTNNAC	6 cut(s)	241, 1712, 1866, 2393, 2794, 3298
Hpy8I	GTNNAC	6 cut(s)	241, 1712, 1866, 2393, 2794, 3298
Hpy99I	CGWCG	4 cut(s)	95, 101, 155, 386
HpyAV	CCTTC	7 cut(s)	209, 218, 581, 847, 2050, 2704, 3196
HpyCH4III	ACNGT	7 cut(s)	101, 233, 359, 595, 904, 1870, 3019
HpyCH4IV	ACGT	5 cut(s)	237, 309, 384, 1967, 3195
HpySE526I	ACGT	5 cut(s)	237, 309, 384, 1967, 3195
Hsp92I	GRCGYC	2 cut(s)	50, 90
HspAI	GCGC	1 cut(s)	50
KasI	GGCGCC	1 cut(s)	49
Kpn2I	TCCGGA	1 cut(s)	2685
KpnI	GGTACC	3 cut(s)	258, 1140, 2981
KroI	GCCGGC	1 cut(s)	444
KroNI	GCCGGC	1 cut(s)	446
Ksp22I	TGATCA	2 cut(s)	282, 2032
KspAI	GTTAAC	1 cut(s)	241
LmnI	GCTCC	6 cut(s)	598, 736, 1588, 1703, 2206, 2362
MaeI	CTAG	7 cut(s)	198, 1092, 2003, 2142, 2346, 2769, 2945
MaeII	ACGT	5 cut(s)	237, 309, 384, 1967, 3195
MaeIII	GTNAC	8 cut(s)	233, 338, 571, 881, 1159, 1436, 1811, 2572
MfeI	CAATTG	1 cut(s)	2838
MflI	RGATCY	6 cut(s)	433, 1018, 1414, 2146, 3033, 3181
MhlI	GDGCHC	5 cut(s)	661, 1714, 2272, 2359, 2395
MlsI	TGGCCA	1 cut(s)	2861
MluNI	TGGCCA	1 cut(s)	2861
Mly113I	GGCGCC	1 cut(s)	50
MlyI	GAGTC	2 cut(s)	1559, 2078
MmeI	TCCRAC	5 cut(s)	43, 435, 1607, 1681, 2322
Mox20I	TGGCCA	1 cut(s)	2861
MroI	TCCGGA	1 cut(s)	2685
MroNI	GCCGGC	1 cut(s)	444
MroXI	GAANNNNTTC	1 cut(s)	1578
MscI	TGGCCA	1 cut(s)	2861
MslI	CAYNNNNRTG	5 cut(s)	1166, 1717, 2102, 2855, 3234
Msp20I	TGGCCA	1 cut(s)	2861
MspA1I	CMGCKG	2 cut(s)	2038, 2314
MspCI	CTTAAG	1 cut(s)	3077
MunI	CAATTG	1 cut(s)	2838
Mva1269I	GAATGC	2 cut(s)	2781, 3100
MvaI	CCWGG	7 cut(s)	755, 2020, 2194, 2266, 2624, 2679, 2879
NaeI	GCCGGC	1 cut(s)	446
NarI	GGCGCC	1 cut(s)	50
NciI	CCSGG	4 cut(s)	467, 1224, 2303, 2418
NcoI	CCATGG	2 cut(s)	1473, 2964
NdeI	CATATG	1 cut(s)	2733
NgoMIV	GCCGGC	1 cut(s)	444
NmeAIII	GCCGAG	1 cut(s)	242
NmuCI	GTSAC	6 cut(s)	233, 571, 881, 1159, 1436, 2572
NspI	RCATGY	1 cut(s)	3142
OliI	CACNNNNGTG	1 cut(s)	1166
PceI	AGGCCT	1 cut(s)	861
PcsI	WCGNNNNNNNCGW	1 cut(s)	306
PctI	GAATGC	2 cut(s)	2781, 3100
PdiI	GCCGGC	1 cut(s)	446
PdmI	GAANNNNTTC	1 cut(s)	1578
PfeI	GAWTC	9 cut(s)	107, 302, 317, 407, 1219, 1574, 1598, 2382, 2698
PflMI	CCANNNNNTGG	1 cut(s)	2914
PleI	GAGTC	2 cut(s)	1559, 2077
PluTI	GGCGCC	1 cut(s)	53
PpsI	GAGTC	2 cut(s)	1559, 2077
Ppu21I	YACGTR	1 cut(s)	1968
PshBI	ATTAAT	4 cut(s)	1383, 1995, 2172, 2631
Psp6I	CCWGG	7 cut(s)	753, 2018, 2192, 2264, 2622, 2677, 2877
PspFI	CCCAGC	2 cut(s)	2177, 2747
PspGI	CCWGG	7 cut(s)	753, 2018, 2192, 2264, 2622, 2677, 2877
PspPI	GGNCC	4 cut(s)	520, 890, 1656, 1937
PstI	CTGCAG	1 cut(s)	2400
PsuI	RGATCY	6 cut(s)	433, 1018, 1414, 2146, 3033, 3181
PvuII	CAGCTG	1 cut(s)	2314
RsaI	GTAC	8 cut(s)	256, 516, 1138, 1464, 1970, 2077, 2350, 2979
RsaNI	GTAC	8 cut(s)	255, 515, 1137, 1463, 1969, 2076, 2349, 2978
RseI	CAYNNNNRTG	5 cut(s)	1166, 1717, 2102, 2855, 3234
Sau96I	GGNCC	4 cut(s)	520, 890, 1656, 1937
ScaI	AGTACT	1 cut(s)	2350
SchI	GAGTC	2 cut(s)	1559, 2078
SduI	GDGCHC	5 cut(s)	661, 1714, 2272, 2359, 2395
SfcI	CTRYAG	6 cut(s)	39, 1664, 1866, 2396, 2610, 2923
SfoI	GGCGCC	1 cut(s)	51
SinI	GGWCC	3 cut(s)	890, 1656, 1937
SmiMI	CAYNNNNRTG	5 cut(s)	1166, 1717, 2102, 2855, 3234
SmlI	CTYRAG	6 cut(s)	1112, 1214, 2066, 2216, 2276, 3077
SmoI	CTYRAG	6 cut(s)	1112, 1214, 2066, 2216, 2276, 3077
SnaBI	TACGTA	1 cut(s)	1968
SpeI	ACTAGT	1 cut(s)	2345
SseBI	AGGCCT	1 cut(s)	861
SsiI	CCGC	6 cut(s)	266, 363, 827, 1460, 1875, 2038
SspDI	GGCGCC	1 cut(s)	49
SspMI	CTAG	7 cut(s)	198, 1092, 2003, 2142, 2346, 2769, 2945
StuI	AGGCCT	1 cut(s)	861
StyI	CCWWGG	6 cut(s)	1073, 1473, 1768, 2202, 2964, 2985
TaaI	ACNGT	7 cut(s)	101, 233, 359, 595, 904, 1870, 3019
TaiI	ACGT	5 cut(s)	240, 312, 387, 1970, 3198
TaqI	TCGA	6 cut(s)	300, 426, 987, 2662, 2706, 3148
TaqII	GACCGA	1 cut(s)	3174
TatI	WGTACW	1 cut(s)	2348
TauI	GCSGC	3 cut(s)	269, 829, 1877
TfiI	GAWTC	9 cut(s)	107, 302, 317, 407, 1219, 1574, 1598, 2382, 2698
TseFI	GTSAC	6 cut(s)	233, 571, 881, 1159, 1436, 2572
Tsp45I	GTSAC	6 cut(s)	233, 571, 881, 1159, 1436, 2572
TspGWI	ACGGA	4 cut(s)	749, 1601, 1826, 3293
Van91I	CCANNNNNTGG	1 cut(s)	2914
Vha464I	CTTAAG	1 cut(s)	3077
VneI	GTGCAC	2 cut(s)	1710, 2391
VpaK11BI	GGWCC	3 cut(s)	890, 1656, 1937
VspI	ATTAAT	4 cut(s)	1383, 1995, 2172, 2631
XagI	CCTNNNNNAGG	1 cut(s)	213
XapI	RAATTY	6 cut(s)	797, 896, 1004, 1301, 1978, 3210
XbaI	TCTAGA	1 cut(s)	2141
XceI	RCATGY	1 cut(s)	3142
XcmI	CCANNNNNNNNNTGG	4 cut(s)	1693, 2200, 2484, 2731
XmiI	GTMKAC	1 cut(s)	1865
XmnI	GAANNNNTTC	1 cut(s)	1578
XspI	CTAG	7 cut(s)	198, 1092, 2003, 2142, 2346, 2769, 2945
ZrmI	AGTACT	1 cut(s)	2350

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.