Basic Information

Type: gene

Biological Identity

Species

rosa_multiflora

Chromosome

Rmu_sc0006171.1

Orthogroup ID

OG0010596

Physical Location & Seq

Genomic Coordinates Reverse (-)

4948 .. 11279

Gene Structure 6332 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1977 bp

atgggtcacttggataaactcaagatggagggtgaagttgtgacatggcccaaaatgaaaataaagtctattatacggaaggacccagttccgagttctgttagtacttggaggcaaatagaatgcagtgagcaaaacgaccctacagtgtgcataatttggggtattgatcttcgcctcctaaagatggctatggggaagtattcccgagttgacggaaagaagtcactgaattgttgctcaacaaccagtctagttgtatttgttgcattttgtttagttggggtttggatgttaatgtcatcagttgttccgattcagagtcaaaacttatcaactgaggagaatctgaccgataaaagttctaagcaatatgaagacagttctggtcgattatcagaaactataacaagagaggatgacagtggcagtgattcacagagtgaaaccaatattgaaaatactcaaaatgtttcacaggacgagtctgtgagtacaagtgacgaaaaacaggaagaagtggtttcaaaggaggtttctgaggagaaagttgagtctgaaagtgagaatggtgacgagggaaatcagcaagagaagtttgtgaaggaaagctctgatgagaaaactgagagcgaagaggaaccaaagacagagaacgaaaatgatggggatgcgaaagtaaatgatcaggaagctagttctggagatggtcagtcaaactcagaagctggatcaacagaggaagcaagtggtaaaacgagtgaaaatgaacagacagaattacaggccagcttcggaggatttctatttgacagagatgttcttgccatgtcatttgctcccaaggatgaacacgaagcccaagttcaatttgcgctagagcggggtatccctgccatatcagctgttatgggtaccacaaggctaccatacccaagttctgtgtttgatcttatccactgtgcacgctgtagagtcccttggcatgttgaaggtggtaaactgctcttggagcttaatcgtgtcttgcgacctggtggttattttgtttggtccgcaactccggtttaccaaaagcttcctgaagatgttggcatctggaaagcgatgtctgaactaactaagtcaatttgctgggatctggtggtgattaaaaaagacaagctcaatcaagtcgctgctgctatatataggaaaccatctaccaatgagtgctacaatgaaagagcacaaaatgagcctcctctttgtagtgaatctgatgatccaaatgctgcctggaaagtaccattagaggcatgcatacacaaagtacctgaggatgcgtcagatcgtgggtctcaatggccaccgcagtggccatcaaggttggagaaaccaccatactggttgaagtctcagcttgatgttaatggaaaatctgcgccagaagattttatttctgactataagcattggaagaatgttgtgtccaagacatatttgactgggatgggaatcgattggtcatctgtaaggaatgtcctggacatgagagctgtatatggagggtttgctgcagcactgaaagacttaaaagtatgggtaatgaatgtagttccgcctattgattctcgtgacacacttgcaataatctttgagcgtggattaatcggaatgtaccatgattggtgtgaatcattcagtacatatcctaggacttatgatgttgtccatgcagatcatctgttctctgtccttaagaaaagatgcaacctagtggcggtgattgcggaagtggacaggatactaaggccagaagggaagctaatcgtatgggatgatgctgaaaccttgaacgagatcgagagcatggtcaaatctctacagtgggatatccgatttacttactcaaaagacaacaagggtttgctctgcattcagaaaacgttttggcgtcctactgagcaagagacaattctatcagccattgcttaa

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005768 GO:0005794 GO:0005802 GO:0005911 GO:0008150 GO:0008152 GO:0008168 GO:0008757 GO:0009506 GO:0012505 GO:0016740 GO:0016741 GO:0030054 GO:0031410 GO:0031982 GO:0031984 GO:0032259 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044431 GO:0044444 GO:0044446 GO:0044464 GO:0055044 GO:0097708 GO:0098791

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Methyltransf_29

Protein Analysis

658

Amino Acids

74.06

Weight (kDa)

4.83

Isoelectric Point (pI)

47.86

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Methyltransf_29	PF03141	263 - 638	9.2e-164	Putative S-adenosyl-L-methionine-dependent methyltransferase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0010596)

Species	Orthologous Gene IDs
malus_domestica	MD06G1039600.v1.1 MD13G1215200.v1.1
prunus_persica	Prupe.5G045100_v2.0.a1
pyrus_communis	pycom05g00430 pycom07g24350 pycom15g02370 pycom16g23870
rosa_chinensis	RchiOBHm_Chr4g0393521
rosa_laevigata	RLG00000006414 RLG00000007546 RLG00000009741 RLG00000012464
rosa_multiflora	Rmu_co8388373.1_g000001 Rmu_sc0004469.1_g000016 Rmu_sc0004884.1_g000002 Rmu_sc0006171.1_g000002
rosa_roxburghii	Rroxscaffold_2G00086270 Rroxscaffold_3G00253570
rosa_samantha	Rh4BG043800 Rh5DG486700 Rh6CG274800

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc65I	GGTACC	1 cut(s)	914
AccB1I	GGYRCC	1 cut(s)	914
AccBSI	CCGCTC	1 cut(s)	883
AciI	CCGC	6 cut(s)	883, 1056, 1352, 1601, 1763, 1772
AclI	AACGTT	1 cut(s)	1928
AclWI	GGATC	3 cut(s)	739, 1146, 1259
AcoI	YGGCCR	2 cut(s)	1346, 1358
AcuI	CTGAAG	1 cut(s)	1104
AcyI	GRCGYC	1 cut(s)	1936
AdeI	CACNNNGTG	1 cut(s)	443
AfaI	GTAC	7 cut(s)	106, 496, 916, 1287, 1314, 1661, 1687
AfiI	CCNNNNNNNGG	2 cut(s)	187, 1762
AflII	CTTAAG	1 cut(s)	1739
AgsI	TTSAA	6 cut(s)	458, 528, 869, 992, 1393, 1837
AjnI	CCWGG	3 cut(s)	1033, 1277, 1524
AleI	CACNNNNGTG	1 cut(s)	1354
Alw21I	GWGCWC	2 cut(s)	967, 1231
Alw26I	GTCTC	3 cut(s)	1344, 1401, 1946
Alw44I	GTGCAC	1 cut(s)	963
AlwI	GGATC	3 cut(s)	739, 1146, 1259
AlwNI	CAGNNNCTG	1 cut(s)	728
Ama87I	CYCGRG	1 cut(s)	207
AoxI	GGCC	5 cut(s)	47, 786, 1346, 1358, 1793
ApaLI	GTGCAC	1 cut(s)	963
ApeKI	GCWGC	5 cut(s)	1178, 1181, 1274, 1556, 1559
AseI	ATTAAT	1 cut(s)	1649
Asp718I	GGTACC	1 cut(s)	914
AspA2I	CCTAGG	1 cut(s)	1694
AspLEI	GCGC	2 cut(s)	877, 1426
AspS9I	GGNCC	3 cut(s)	48, 82, 1053
AsuHPI	GGTGA	4 cut(s)	44, 584, 1159, 1777
AvaI	CYCGRG	1 cut(s)	207
AvaII	GGWCC	2 cut(s)	82, 1053
AvrII	CCTAGG	1 cut(s)	1694
AxyI	CCTNAGG	1 cut(s)	1317
BaeGI	GKGCMC	1 cut(s)	967
BalI	TGGCCA	2 cut(s)	1348, 1360
BanI	GGYRCC	1 cut(s)	914
BauI	CACGAG	1 cut(s)	1614
BbsI	GAAGAC	1 cut(s)	384
Bbv12I	GWGCWC	2 cut(s)	967, 1231
BbvI	GCAGC	5 cut(s)	1165, 1168, 1261, 1543, 1571
BccI	CCATC	7 cut(s)	19, 181, 659, 701, 1207, 1369, 1486
BciT130I	CCWGG	3 cut(s)	1035, 1279, 1526
BciVI	GTATCC	2 cut(s)	899, 1779
BclI	TGATCA	1 cut(s)	685
BcoDI	GTCTC	3 cut(s)	1344, 1401, 1946
BfaI	CTAG	5 cut(s)	254, 696, 878, 1695, 1757
BfmI	CTRYAG	4 cut(s)	144, 970, 1557, 1865
BfrI	CTTAAG	1 cut(s)	1739
BfuI	GTATCC	2 cut(s)	899, 1779
BisI	GCNGC	5 cut(s)	1179, 1182, 1275, 1557, 1560
BlnI	CCTAGG	1 cut(s)	1694
BlsI	GCNGC	5 cut(s)	1180, 1183, 1276, 1558, 1561
BmcAI	AGTACT	1 cut(s)	106
Bme1390I	CCNGG	3 cut(s)	1035, 1279, 1526
Bme18I	GGWCC	2 cut(s)	82, 1053
BmeT110I	CYCGRG	1 cut(s)	207
BmgT120I	GGNCC	3 cut(s)	48, 82, 1053
BmiI	GGNNCC	3 cut(s)	84, 642, 916
BmrFI	CCNGG	3 cut(s)	1035, 1279, 1526
BmrI	ACTGGG	2 cut(s)	80, 1497
BmsI	GCATC	5 cut(s)	661, 1104, 1312, 1739, 1813
BmuI	ACTGGG	2 cut(s)	80, 1497
BpiI	GAAGAC	1 cut(s)	384
BpmI	CTGGAG	1 cut(s)	723
BpuEI	CTTGAG	1 cut(s)	5
Bsa29I	ATCGAT	1 cut(s)	1500
BsaBI	GATNNNNATC	1 cut(s)	1496
BsaHI	GRCGYC	1 cut(s)	1936
BsaI	GGTCTC	1 cut(s)	1344
BsaJI	CCNNGG	3 cut(s)	843, 980, 1694
BsaWI	WCCGGW	1 cut(s)	1063
Bsc4I	CCNNNNNNNGG	2 cut(s)	187, 1762
Bse1I	ACTGG	4 cut(s)	86, 249, 1391, 1492
Bse21I	CCTNAGG	1 cut(s)	1317
Bse3DI	GCAATG	1 cut(s)	1968
Bse8I	GATNNNNATC	1 cut(s)	1496
BseBI	CCWGG	3 cut(s)	1035, 1279, 1526
BseCI	ATCGAT	1 cut(s)	1500
BseDI	CCNNGG	3 cut(s)	843, 980, 1694
BseGI	GGATG	7 cut(s)	297, 424, 676, 853, 1327, 1497, 1825
BseJI	GATNNNNATC	1 cut(s)	1496
BseLI	CCNNNNNNNGG	2 cut(s)	187, 1762
BseMI	GCAATG	1 cut(s)	1968
BseMII	CTCAG	7 cut(s)	330, 531, 618, 735, 1308, 1412, 1935
BseNI	ACTGG	4 cut(s)	86, 249, 1391, 1492
BseRI	GAGGAG	3 cut(s)	356, 557, 1233
BseSI	GKGCMC	1 cut(s)	967
BseXI	GCAGC	5 cut(s)	1165, 1168, 1261, 1543, 1571
BseYI	CCCAGC	1 cut(s)	1134
BshFI	GGCC	5 cut(s)	49, 788, 1348, 1360, 1795
BshNI	GGYRCC	1 cut(s)	914
BshVI	ATCGAT	1 cut(s)	1500
BsiHKAI	GWGCWC	2 cut(s)	967, 1231
BsiHKCI	CYCGRG	1 cut(s)	207
BsiSI	CCGG	1 cut(s)	1064
BslFI	GGGAC	1 cut(s)	962
BslI	CCNNNNNNNGG	2 cut(s)	187, 1762
BsmAI	GTCTC	3 cut(s)	1344, 1401, 1946
BsmFI	GGGAC	1 cut(s)	962
BsmI	GAATGC	2 cut(s)	128, 1917
BsnI	GGCC	5 cut(s)	49, 788, 1348, 1360, 1795
Bso31I	GGTCTC	1 cut(s)	1344
BsoBI	CYCGRG	1 cut(s)	207
Bsp1286I	GDGCHC	2 cut(s)	967, 1231
Bsp143I	GATC	9 cut(s)	169, 685, 731, 949, 1138, 1264, 1330, 1720, 1842
BspACI	CCGC	6 cut(s)	883, 1056, 1352, 1601, 1763, 1772
BspANI	GGCC	5 cut(s)	49, 788, 1348, 1360, 1795
BspCNI	CTCAG	7 cut(s)	331, 532, 619, 734, 1309, 1411, 1936
BspDI	ATCGAT	1 cut(s)	1500
BspLI	GGNNCC	3 cut(s)	84, 642, 916
BspMAI	CTGCAG	1 cut(s)	1561
BspPI	GGATC	3 cut(s)	739, 1146, 1259
BspT107I	GGYRCC	1 cut(s)	914
BspTI	CTTAAG	1 cut(s)	1739
BspTNI	GGTCTC	1 cut(s)	1344
BsrBI	CCGCTC	1 cut(s)	883
BsrDI	GCAATG	1 cut(s)	1968
BsrI	ACTGG	4 cut(s)	86, 249, 1391, 1492
BssECI	CCNNGG	3 cut(s)	843, 980, 1694
BssMI	GATC	9 cut(s)	169, 685, 731, 949, 1138, 1264, 1330, 1720, 1842
BssNI	GRCGYC	1 cut(s)	1936
BssSI	CACGAG	1 cut(s)	1614
BssT1I	CCWWGG	3 cut(s)	843, 980, 1694
Bst2BI	CACGAG	1 cut(s)	1614
Bst2UI	CCWGG	3 cut(s)	1035, 1279, 1526
Bst4CI	ACNGT	5 cut(s)	148, 383, 425, 962, 1869
Bst6I	CTCTTC	1 cut(s)	630
BstACI	GRCGYC	1 cut(s)	1936
BstAFI	CTTAAG	1 cut(s)	1739
BstC8I	GCNNGC	3 cut(s)	790, 967, 1300
BstF5I	GGATG	7 cut(s)	297, 424, 676, 853, 1327, 1497, 1825
BstHHI	GCGC	2 cut(s)	877, 1426
BstKTI	GATC	9 cut(s)	172, 688, 734, 952, 1141, 1267, 1333, 1723, 1845
BstMAI	GTCTC	3 cut(s)	1344, 1401, 1946
BstMBI	GATC	9 cut(s)	169, 685, 731, 949, 1138, 1264, 1330, 1720, 1842
BstMWI	GCNNNNNNNGC	3 cut(s)	902, 1012, 1769
BstNI	CCWGG	3 cut(s)	1035, 1279, 1526
BstNSI	RCATGY	2 cut(s)	989, 1302
BstSCI	CCNGG	3 cut(s)	1033, 1277, 1524
BstSFI	CTRYAG	4 cut(s)	144, 970, 1557, 1865
BstSLI	GKGCMC	1 cut(s)	967
BstV1I	GCAGC	5 cut(s)	1165, 1168, 1261, 1543, 1571
BstV2I	GAAGAC	1 cut(s)	384
BstX2I	RGATCY	1 cut(s)	1138
BstXI	CCANNNNNNTGG	2 cut(s)	1356, 1386
BstYI	RGATCY	1 cut(s)	1138
Bsu15I	ATCGAT	1 cut(s)	1500
Bsu36I	CCTNAGG	1 cut(s)	1317
BsuI	GTATCC	2 cut(s)	899, 1779
BsuRI	GGCC	5 cut(s)	49, 788, 1348, 1360, 1795
BsuTUI	ATCGAT	1 cut(s)	1500
BtgZI	GCGATG	1 cut(s)	1121
BtsCI	GGATG	7 cut(s)	297, 424, 676, 853, 1327, 1497, 1825
BtsI	GCAGTG	3 cut(s)	133, 436, 1361
BtsIMutI	CAGTG	9 cut(s)	133, 153, 227, 430, 436, 958, 1361, 1562, 1874
Cac8I	GCNNGC	3 cut(s)	790, 967, 1300
CaiI	CAGNNNCTG	1 cut(s)	728
CfoI	GCGC	2 cut(s)	877, 1426
Cfr13I	GGNCC	3 cut(s)	48, 82, 1053
ClaI	ATCGAT	1 cut(s)	1500
CseI	GACGC	2 cut(s)	1314, 1925
CsiI	ACCWGGT	1 cut(s)	1033
Csp6I	GTAC	7 cut(s)	105, 495, 915, 1286, 1313, 1660, 1686
CviAII	CATG	8 cut(s)	45, 829, 986, 1299, 1531, 1664, 1715, 1852
CviQI	GTAC	7 cut(s)	105, 495, 915, 1286, 1313, 1660, 1686
DpnI	GATC	9 cut(s)	171, 687, 733, 951, 1140, 1266, 1332, 1722, 1844
DpnII	GATC	9 cut(s)	169, 685, 731, 949, 1138, 1264, 1330, 1720, 1842
DraIII	CACNNNGTG	1 cut(s)	443
EaeI	YGGCCR	2 cut(s)	1346, 1358
Eam1104I	CTCTTC	1 cut(s)	630
EarI	CTCTTC	1 cut(s)	630
EciI	GGCGGA	1 cut(s)	1590
Eco130I	CCWWGG	3 cut(s)	843, 980, 1694
Eco31I	GGTCTC	1 cut(s)	1344
Eco32I	GATATC	1 cut(s)	1876
Eco47I	GGWCC	2 cut(s)	82, 1053
Eco57I	CTGAAG	1 cut(s)	1104
Eco81I	CCTNAGG	1 cut(s)	1317
Eco88I	CYCGRG	1 cut(s)	207
EcoO109I	RGGNCCY	1 cut(s)	82
EcoRII	CCWGG	3 cut(s)	1033, 1277, 1524
EcoRV	GATATC	1 cut(s)	1876
EcoT14I	CCWWGG	3 cut(s)	843, 980, 1694
EcoT22I	ATGCAT	1 cut(s)	1304
ErhI	CCWWGG	3 cut(s)	843, 980, 1694
FaeI	CATG	8 cut(s)	48, 832, 989, 1302, 1534, 1667, 1718, 1855
FaqI	GGGAC	1 cut(s)	962
FatI	CATG	8 cut(s)	44, 828, 985, 1298, 1530, 1663, 1714, 1851
FauI	CCCGC	1 cut(s)	876
FbaI	TGATCA	1 cut(s)	685
Fnu4HI	GCNGC	5 cut(s)	1179, 1182, 1275, 1557, 1560
FokI	GGATG	7 cut(s)	304, 431, 683, 860, 1334, 1504, 1832
Fsp4HI	GCNGC	5 cut(s)	1179, 1182, 1275, 1557, 1560
FspBI	CTAG	5 cut(s)	254, 696, 878, 1695, 1757
GlaI	GCGC	2 cut(s)	876, 1425
GluI	GCNGC	5 cut(s)	1179, 1182, 1275, 1557, 1560
GsaI	CCCAGC	1 cut(s)	1138
GsuI	CTGGAG	1 cut(s)	723
HaeIII	GGCC	5 cut(s)	49, 788, 1348, 1360, 1795
HapII	CCGG	1 cut(s)	1064
HgaI	GACGC	2 cut(s)	1314, 1925
HhaI	GCGC	2 cut(s)	877, 1426
Hin1I	GRCGYC	1 cut(s)	1936
Hin1II	CATG	8 cut(s)	48, 832, 989, 1302, 1534, 1667, 1718, 1855
Hin6I	GCGC	2 cut(s)	875, 1424
HinP1I	GCGC	2 cut(s)	875, 1424
HincII	GTYRAC	1 cut(s)	214
HindII	GTYRAC	1 cut(s)	214
HindIII	AAGCTT	1 cut(s)	1076
HpaII	CCGG	1 cut(s)	1064
HphI	GGTGA	4 cut(s)	44, 584, 1159, 1777
Hpy166II	GTNNAC	5 cut(s)	214, 965, 1001, 1069, 1780
Hpy188III	TCNNGA	8 cut(s)	22, 207, 689, 702, 1082, 1099, 1616, 1846
Hpy8I	GTNNAC	5 cut(s)	214, 965, 1001, 1069, 1780
HpyAV	CCTTC	4 cut(s)	73, 598, 986, 1793
HpyCH4III	ACNGT	5 cut(s)	148, 383, 425, 962, 1869
HpyCH4IV	ACGT	1 cut(s)	1928
HpyF10VI	GCNNNNNNNGC	3 cut(s)	902, 1012, 1769
HpySE526I	ACGT	1 cut(s)	1928
Hsp92I	GRCGYC	1 cut(s)	1936
Hsp92II	CATG	8 cut(s)	48, 832, 989, 1302, 1534, 1667, 1718, 1855
HspAI	GCGC	2 cut(s)	875, 1424
KpnI	GGTACC	1 cut(s)	918
Ksp22I	TGATCA	1 cut(s)	685
Kzo9I	GATC	9 cut(s)	169, 685, 731, 949, 1138, 1264, 1330, 1720, 1842
LmnI	GCTCC	2 cut(s)	844, 1012
Lsp1109I	GCAGC	5 cut(s)	1165, 1168, 1261, 1543, 1571
LweI	GCATC	5 cut(s)	661, 1104, 1312, 1739, 1813
MabI	ACCWGGT	1 cut(s)	1033
MaeI	CTAG	5 cut(s)	254, 696, 878, 1695, 1757
MaeII	ACGT	1 cut(s)	1928
MaeIII	GTNAC	6 cut(s)	5, 40, 225, 500, 572, 1616
MalI	GATC	9 cut(s)	171, 687, 733, 951, 1140, 1266, 1332, 1722, 1844
MbiI	CCGCTC	1 cut(s)	883
MboI	GATC	9 cut(s)	169, 685, 731, 949, 1138, 1264, 1330, 1720, 1842
MboII	GAAGA	7 cut(s)	164, 389, 527, 647, 1097, 1442, 1471
MflI	RGATCY	1 cut(s)	1138
MhlI	GDGCHC	2 cut(s)	967, 1231
MlsI	TGGCCA	2 cut(s)	1348, 1360
MluCI	AATT	6 cut(s)	156, 232, 779, 869, 1128, 1956
MluNI	TGGCCA	2 cut(s)	1348, 1360
MlyI	GAGTC	4 cut(s)	331, 494, 563, 984
MmeI	TCCRAC	1 cut(s)	1350
Mox20I	TGGCCA	2 cut(s)	1348, 1360
Mph1103I	ATGCAT	1 cut(s)	1304
MscI	TGGCCA	2 cut(s)	1348, 1360
MseI	TTAA	8 cut(s)	296, 1017, 1152, 1410, 1574, 1649, 1740, 1975
MslI	CAYNNNNRTG	1 cut(s)	1354
Msp20I	TGGCCA	2 cut(s)	1348, 1360
MspA1I	CMGCKG	1 cut(s)	905
MspCI	CTTAAG	1 cut(s)	1739
MspI	CCGG	1 cut(s)	1064
MspR9I	CCNGG	3 cut(s)	1035, 1279, 1526
Mva1269I	GAATGC	2 cut(s)	128, 1917
MvaI	CCWGG	3 cut(s)	1035, 1279, 1526
MwoI	GCNNNNNNNGC	3 cut(s)	902, 1012, 1769
NdeII	GATC	9 cut(s)	169, 685, 731, 949, 1138, 1264, 1330, 1720, 1842
NlaIII	CATG	8 cut(s)	48, 832, 989, 1302, 1534, 1667, 1718, 1855
NlaIV	GGNNCC	3 cut(s)	84, 642, 916
NmuCI	GTSAC	6 cut(s)	5, 40, 225, 500, 572, 1616
NsiI	ATGCAT	1 cut(s)	1304
NspI	RCATGY	2 cut(s)	989, 1302
OliI	CACNNNNGTG	1 cut(s)	1354
PaeI	GCATGC	1 cut(s)	1302
PcsI	WCGNNNNNNNCGW	1 cut(s)	1027
PctI	GAATGC	2 cut(s)	128, 1917
PfeI	GAWTC	7 cut(s)	316, 346, 434, 1256, 1497, 1610, 1676
PfoI	TCCNGGA	1 cut(s)	1524
PkrI	GCNGC	5 cut(s)	1180, 1183, 1276, 1558, 1561
PleI	GAGTC	4 cut(s)	330, 493, 562, 983
PpsI	GAGTC	4 cut(s)	330, 493, 562, 983
PpuMI	RGGWCCY	1 cut(s)	82
PshBI	ATTAAT	1 cut(s)	1649
Psp1406I	AACGTT	1 cut(s)	1928
Psp5II	RGGWCCY	1 cut(s)	82
Psp6I	CCWGG	3 cut(s)	1033, 1277, 1524
PspFI	CCCAGC	1 cut(s)	1134
PspGI	CCWGG	3 cut(s)	1033, 1277, 1524
PspN4I	GGNNCC	3 cut(s)	84, 642, 916
PspPI	GGNCC	3 cut(s)	48, 82, 1053
PspPPI	RGGWCCY	1 cut(s)	82
PstI	CTGCAG	1 cut(s)	1561
PstNI	CAGNNNCTG	1 cut(s)	728
PsuI	RGATCY	1 cut(s)	1138
PvuII	CAGCTG	1 cut(s)	905
RsaI	GTAC	7 cut(s)	106, 496, 916, 1287, 1314, 1661, 1687
RsaNI	GTAC	7 cut(s)	105, 495, 915, 1286, 1313, 1660, 1686
RseI	CAYNNNNRTG	1 cut(s)	1354
SaqAI	TTAA	8 cut(s)	296, 1017, 1152, 1410, 1574, 1649, 1740, 1975
SatI	GCNGC	5 cut(s)	1179, 1182, 1275, 1557, 1560
Sau3AI	GATC	9 cut(s)	169, 685, 731, 949, 1138, 1264, 1330, 1720, 1842
Sau96I	GGNCC	3 cut(s)	48, 82, 1053
ScaI	AGTACT	1 cut(s)	106
SchI	GAGTC	4 cut(s)	331, 494, 563, 984
ScrFI	CCNGG	3 cut(s)	1035, 1279, 1526
SduI	GDGCHC	2 cut(s)	967, 1231
SexAI	ACCWGGT	1 cut(s)	1033
SfaNI	GCATC	5 cut(s)	661, 1104, 1312, 1739, 1813
SfcI	CTRYAG	4 cut(s)	144, 970, 1557, 1865
SinI	GGWCC	2 cut(s)	82, 1053
SmiMI	CAYNNNNRTG	1 cut(s)	1354
SmlI	CTYRAG	2 cut(s)	20, 1739
SmoI	CTYRAG	2 cut(s)	20, 1739
SphI	GCATGC	1 cut(s)	1302
Sse9I	AATT	6 cut(s)	156, 232, 779, 869, 1128, 1956
SsiI	CCGC	6 cut(s)	883, 1056, 1352, 1601, 1763, 1772
SspI	AATATT	1 cut(s)	454
SspMI	CTAG	5 cut(s)	254, 696, 878, 1695, 1757
StyD4I	CCNGG	3 cut(s)	1033, 1277, 1524
StyI	CCWWGG	3 cut(s)	843, 980, 1694
TaaI	ACNGT	5 cut(s)	148, 383, 425, 962, 1869
TaiI	ACGT	1 cut(s)	1931
TaqI	TCGA	3 cut(s)	391, 1500, 1845
TaqII	GACCGA	1 cut(s)	368
TasI	AATT	6 cut(s)	156, 232, 779, 869, 1128, 1956
TatI	WGTACW	3 cut(s)	104, 494, 1685
TfiI	GAWTC	7 cut(s)	316, 346, 434, 1256, 1497, 1610, 1676
Tru1I	TTAA	8 cut(s)	296, 1017, 1152, 1410, 1574, 1649, 1740, 1975
Tru9I	TTAA	8 cut(s)	296, 1017, 1152, 1410, 1574, 1649, 1740, 1975
TscAI	CASTG	9 cut(s)	133, 153, 234, 430, 436, 965, 1361, 1569, 1874
TseFI	GTSAC	6 cut(s)	5, 40, 225, 500, 572, 1616
TseI	GCWGC	5 cut(s)	1178, 1181, 1274, 1556, 1559
Tsp45I	GTSAC	6 cut(s)	5, 40, 225, 500, 572, 1616
TspDTI	ATGAA	6 cut(s)	71, 390, 783, 864, 1236, 1604
TspGWI	ACGGA	2 cut(s)	91, 231
TspRI	CASTG	9 cut(s)	133, 153, 234, 430, 436, 965, 1361, 1569, 1874
Vha464I	CTTAAG	1 cut(s)	1739
VneI	GTGCAC	1 cut(s)	963
VpaK11BI	GGWCC	2 cut(s)	82, 1053
VspI	ATTAAT	1 cut(s)	1649
XceI	RCATGY	2 cut(s)	989, 1302
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1275
XmaJI	CCTAGG	1 cut(s)	1694
XspI	CTAG	5 cut(s)	254, 696, 878, 1695, 1757
ZrmI	AGTACT	1 cut(s)	106
Zsp2I	ATGCAT	1 cut(s)	1304

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.