Rmu_sc0006715.1_g000003

Ubiquitin-activating enzyme E1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0006715.1
OG0002217
Physical Location & Seq
Reverse (-)
9649 .. 15215
5567 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 3324 bp
ctgaaccctacgccacctttccaatattcgcaccgccgcatcctcggcttctcctccgcacgttcttccagctcatcgcactatatgcttcctagaaagagacaagtgggcggagaggttgtagtagaagaaggggaaccagatttcgccaacgaatctccaatcaagaagctttgcaccgacgaatctaagggcagtgactgcaaaaacactggtaacagcaatatcagcagcagcaacagtagcagcagcgtcaagccgccgatcatggcgttaggaaatgggaattcaggggatattgatgaggatctccacagccggcagcttgctgtgtacggccgtgagacgatgaggcggctctttgcctccaacattcttgtctctgggatgcaaggccttggtgccgagattgcaaagaaccttgttcttgccggtgtcaagtctgtgaccttgcatgatgaaggagttgttgagttgtgggacctgtctggtaatttctttttcactgaagacgatattggaaagaatcgggcccttgcttgtgtccaaaaattgcaagaacttaataatgctgtgctcatttcgactttgacaactcagctgactaaagaaaaactttctgatttccaggctgtagttttcactgatatcagcttagacaaagcaattgaattcgatgattactgccataatcatcagcctccaatctcttttatcaaaactgaagtatggggcctttttggcagtgtgttttgtgactttggtcctgagttcactgttcttgatgttgatggtgaggatccacacacaggcatagtcgcatcaattagcaatgacaaccctgctatgatatcatgtgtcgatgatgagaggcttgagtttcaggacggggatttggttctgttcactgaagtccatggaatgaccgagttgaatgacggaaagccgaggaagattaagaatgcaaggccgtattcattcacaattgagcaggacactacaaattatgcaggatatgagaaaggtggtatagttacacaggttaagcaacccaaggtgttgaaatttaagcccttgcgagaagcagtcaaggaacctggtgatttccttctgattgacttctccaagtttgatcggccacctctcctgcacttggcatttcaggcactagataagtttatatcagagttaggacgcttccctgttgctggatccgaggatgatgctacaaagttcatatcattggtaactagcataaatgatggctcggcagatgggaggcttgaggagattgatcagaagattcttcgtcactttgcatttggtgctagggctgtgctgaatcccatggctgcaatgtttggtggtattgttggacaggaggttgtgaaggcctgttctgcaaagtttcaccctcttttccagtttttctatttcgattcagttgaatcacttccgacagaagccttggaccctagtgatttgaagcctctaaacagtcggtatgatgcacaaatttcagtatttggagccaagctgcagaaaaagctggaggattcaaaagtgttcactgttgggtctggtgcactaggatgcgagtttttgaagaatttagctttgatgggcgttgcttgtggtcaagaaggtaaattaacaattacagatgatgatgtcattgagaagagtaaccttagtagacaatttctcttccgggattggaacattgggcaggctaaatcaacagttgctgccactgctgctgcacttataaacagtcgtatcaatattgaagcactgcagaatcgtgcaagcccagagactgaaaacgtgtttgatgatactttctgggagaatttggatgttgttatcaatgctctagataatgtgaacgctaggctttatattgatcagcgatgcttgtatttccagaagccacttttggaatcaggtaccctaggtgccaagtgcaacacacagatggtcattcctcacttgactgaaaattatggagcatcacgggacccacctgaaaagcaagcacctatgtgcacagttcattcatttcctcacaacattgaccactgcttgacatgggctcgttctgagtttgagggtttacttgagaaggtcccagctgaagtaaatgcctacttgacaaatcctagtgagtacactactgcaatgaagaatgctggtgatgctcaggcaaggaataacttggaaagtgtcattgaatgccttgacaaggagagatgcgagacatttcaagattgcataacctgggcccgactaaagtttgaggactacttttccaaccgtgtgaagcagttaacatatacttttcctgaggatgctacaaccagtagtgggaccccattctggtctgctcccaagcgtttccctcgcccactggtgttctcagtagatgatctcagccaccttcaatttgtactggcagcatccatactacgagctgagacatttaacattgcaattcctgattggatcaagtctactcaaaagtttgctgatgctgttaacaacgtgatggtacctgaatttcagcctaagaaggatgtgaagattgtaatagatgagaaagctacaaacattctaccggcatccatcgatgatgctgctgttatcaacgagttagttatgaagttagaaaaatgcagggagcagctcccaccagccttcaagatgaaccctattcaatttgagaaggatgatgacacaaactatcatatggatgtaatagctggatttgcaaatatgagggctaggaactatagtatccctgaagtcgacaagctgaaggccaaattcatcgccggtagaatcattcctgcaattgcaacatccactgctcttgcaactggtcttgtctgcttggagctgtacaaggttctggccggagagcacaagatagaggactaccgaaacacctttgccaatcttgctcttcctctgttctccatggctgaaccagtacctcctaaagtcatcaagcaccaagatatgagctggacagtttgggacagatggattataaaggacaacccaactttgaagcagctccttaattggctcaaagaaaagggcttaaacgcttacagcatctcttatggcagttgccttctgtataacagcatgttccccaagcacagagagcgcatggacaagcatatggtggatttggctagggatgtggcaaaggcggagctgcctccatatcggaaccactttgatgtggtggtggcctgcgaagatgatgaggataatgatattgacatccctcagatctccatttacttcaagtag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04120 ko05012 map04120 map05012
Pfam Domains
Protein Families
E1_4HB E1_FCCH E1_UFD ThiF UBA_e1_thiolCys

Protein Analysis

1107

Amino Acids

123.74

Weight (kDa)

5.15

Isoelectric Point (pI)

38.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ThiF PF00899 105 - 479 1.4e-24 ThiF family
E1_FCCH PF16190 279 - 349 5e-28 Ubiquitin-activating enzyme E1 FCCH domain
E1_4HB PF16191 350 - 420 1.3e-24 Ubiquitin-activating enzyme E1 four-helix bundle
ThiF PF00899 502 - 994 4.4e-61 ThiF family
UBA_E1_SCCH PF10585 689 - 942 1.8e-86 Ubiquitin-activating enzyme, SCCH domain
E1_UFD PF09358 1013 - 1102 6.5e-25 Ubiquitin fold domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1769, 3053
Acc65I GGTACC 2 cut(s) 1949, 2551
AccB1I GGYRCC 4 cut(s) 401, 1949, 1958, 2551
AccI GTMKAC 3 cut(s) 1696, 2513, 2808
AciI CCGC 7 cut(s) 34, 37, 57, 111, 260, 355, 3221
AclWI GGATC 6 cut(s) 315, 794, 807, 1206, 1219, 2513
AcoI YGGCCR 3 cut(s) 337, 1136, 2913
AcsI RAATTY 8 cut(s) 286, 671, 1064, 1515, 1609, 1852, 2558, 2825
AcuI CTGAAG 6 cut(s) 528, 744, 930, 2157, 2823, 2837
AfaI GTAC 7 cut(s) 335, 1951, 2171, 2451, 2553, 2903, 2994
AfiI CCNNNNNNNGG 6 cut(s) 809, 1153, 1208, 2245, 2367, 2379
AflIII ACRYGT 1 cut(s) 1827
AjnI CCWGG 3 cut(s) 627, 1096, 2279
AjuI GAANNNNNNNTTGG 4 cut(s) 501, 533, 1922, 1954
AleI CACNNNNGTG 1 cut(s) 2044
Alw21I GWGCWC 4 cut(s) 579, 1588, 2051, 2925
Alw26I GTCTC 6 cut(s) 94, 338, 385, 1811, 2252, 2471
Alw44I GTGCAC 2 cut(s) 1584, 2047
AlwI GGATC 6 cut(s) 315, 794, 807, 1206, 1219, 2513
AlwNI CAGNNNCTG 3 cut(s) 201, 1748, 1820
ApaI GGGCCC 2 cut(s) 535, 2287
ApaLI GTGCAC 2 cut(s) 1584, 2047
ApoI RAATTY 8 cut(s) 286, 671, 1064, 1515, 1609, 1852, 2558, 2825
Asp700I GAANNNNTTC 1 cut(s) 1452
Asp718I GGTACC 2 cut(s) 1949, 2551
AspA2I CCTAGG 1 cut(s) 1954
AspLEI GCGC 1 cut(s) 3177
AsuC2I CCSGG 1 cut(s) 1712
AsuHPI GGTGA 4 cut(s) 806, 1112, 1403, 2207
AvaII GGWCC 6 cut(s) 481, 764, 1471, 2020, 2128, 2370
AvrII CCTAGG 1 cut(s) 1954
AxyI CCTNAGG 1 cut(s) 2346
BaeGI GKGCMC 4 cut(s) 535, 1588, 2051, 2287
BaeI ACNNNNGTAYC 4 cut(s) 1762, 1795, 2779, 2812
BamHI GGATCC 2 cut(s) 799, 1211
BanI GGYRCC 4 cut(s) 401, 1949, 1958, 2551
BanII GRGCYC 3 cut(s) 535, 2098, 2287
BbsI GAAGAC 1 cut(s) 516
Bbv12I GWGCWC 4 cut(s) 579, 1588, 2051, 2925
BccI CCATC 8 cut(s) 785, 1256, 1268, 1615, 1972, 2542, 2633, 3039
BceAI ACGGC 3 cut(s) 324, 352, 955
BcgI CGANNNNNNTGC 4 cut(s) 1205, 1239, 2618, 2652
BciT130I CCWGG 3 cut(s) 629, 1098, 2281
BciVI GTATCC 1 cut(s) 2807
BclI TGATCA 2 cut(s) 1294, 1906
BcnI CCSGG 1 cut(s) 1712
BcoDI GTCTC 6 cut(s) 94, 338, 385, 1811, 2252, 2471
BfmI CTRYAG 4 cut(s) 633, 1538, 1796, 2791
BfuI GTATCC 1 cut(s) 2807
BglI GCCNNNNNGGC 1 cut(s) 741
BglII AGATCT 1 cut(s) 3303
BlnI CCTAGG 1 cut(s) 1954
Bme1390I CCNGG 4 cut(s) 629, 1098, 1712, 2281
Bme18I GGWCC 6 cut(s) 481, 764, 1471, 2020, 2128, 2370
BmrFI CCNGG 4 cut(s) 629, 1098, 1712, 2281
BoxI GACNNNNGTC 1 cut(s) 762
BpiI GAAGAC 1 cut(s) 516
BpmI CTGGAG 1 cut(s) 1571
Bpu10I CCTNAGC 1 cut(s) 2202
BpuEI CTTGAG 3 cut(s) 896, 1304, 2141
BpuMI CCSGG 1 cut(s) 1712
Bsa29I ATCGAT 1 cut(s) 2628
BsaBI GATNNNNATC 1 cut(s) 306
BsaXI ACNNNNNCTCC 2 cut(s) 105, 135
Bsc4I CCNNNNNNNGG 6 cut(s) 809, 1153, 1208, 2245, 2367, 2379
Bse118I RCCGGY 4 cut(s) 318, 431, 2617, 2834
Bse1I ACTGG 7 cut(s) 217, 1423, 2361, 2415, 2457, 2884, 2990
Bse21I CCTNAGG 1 cut(s) 2346
Bse3DI GCAATG 4 cut(s) 838, 1362, 2187, 2487
Bse8I GATNNNNATC 1 cut(s) 306
BseBI CCWGG 3 cut(s) 629, 1098, 2281
BseCI ATCGAT 1 cut(s) 2628
BseJI GATNNNNATC 1 cut(s) 306
BseLI CCNNNNNNNGG 6 cut(s) 809, 1153, 1208, 2245, 2367, 2379
BseMI GCAATG 4 cut(s) 838, 1362, 2187, 2487
BseMII CTCAG 9 cut(s) 611, 759, 2094, 2216, 2337, 2433, 2446, 2466, 3314
BseNI ACTGG 7 cut(s) 217, 1423, 2361, 2415, 2457, 2884, 2990
BseRI GAGGAG 2 cut(s) 43, 1301
BseSI GKGCMC 4 cut(s) 535, 1588, 2051, 2287
BseX3I CGGCCG 1 cut(s) 337
BseYI CCCAGC 1 cut(s) 2131
BsgI GTGCAG 2 cut(s) 1133, 1746
Bsh1285I CGRYCG 1 cut(s) 340
BshNI GGYRCC 4 cut(s) 401, 1949, 1958, 2551
BshVI ATCGAT 1 cut(s) 2628
BsiEI CGRYCG 1 cut(s) 340
BsiHKAI GWGCWC 4 cut(s) 579, 1588, 2051, 2925
BsiSI CCGG 6 cut(s) 319, 432, 1711, 2618, 2835, 2916
BslFI GGGAC 5 cut(s) 494, 2033, 2114, 2383, 3053
BslI CCNNNNNNNGG 6 cut(s) 809, 1153, 1208, 2245, 2367, 2379
BsmAI GTCTC 6 cut(s) 94, 338, 385, 1811, 2252, 2471
BsmBI CGTCTC 1 cut(s) 338
BsmFI GGGAC 5 cut(s) 494, 2033, 2114, 2383, 3053
BsmI GAATGC 3 cut(s) 967, 2194, 2240
Bsp120I GGGCCC 2 cut(s) 531, 2283
Bsp1286I GDGCHC 7 cut(s) 535, 579, 1588, 2051, 2098, 2287, 2925
Bsp1407I TGTACA 1 cut(s) 2901
Bsp19I CCATGG 3 cut(s) 916, 1347, 2979
BspACI CCGC 7 cut(s) 34, 37, 57, 111, 260, 355, 3221
BspCNI CTCAG 9 cut(s) 610, 760, 2095, 2215, 2338, 2432, 2445, 2467, 3313
BspDI ATCGAT 1 cut(s) 2628
BspMAI CTGCAG 2 cut(s) 1542, 1800
BspPI GGATC 6 cut(s) 315, 794, 807, 1206, 1219, 2513
BspQI GCTCTTC 1 cut(s) 2970
BspT107I GGYRCC 4 cut(s) 401, 1949, 1958, 2551
BsrDI GCAATG 4 cut(s) 838, 1362, 2187, 2487
BsrFI RCCGGY 4 cut(s) 318, 431, 2617, 2834
BsrGI TGTACA 1 cut(s) 2901
BsrI ACTGG 7 cut(s) 217, 1423, 2361, 2415, 2457, 2884, 2990
BssAI RCCGGY 4 cut(s) 318, 431, 2617, 2834
BssT1I CCWWGG 7 cut(s) 397, 916, 1053, 1347, 1467, 1954, 2979
Bst2UI CCWGG 3 cut(s) 629, 1098, 2281
Bst4CI ACNGT 9 cut(s) 242, 778, 1499, 1573, 1744, 1775, 2053, 2318, 3034
Bst6I CTCTTC 3 cut(s) 1676, 1712, 2970
BstAPI GCANNNNNTGC 3 cut(s) 85, 201, 1325
BstAUI TGTACA 1 cut(s) 2901
BstC8I GCNNGC 6 cut(s) 320, 327, 1731, 1810, 2037, 3265
BstDSI CCRYGG 3 cut(s) 916, 1347, 2979
BstENI CCTNNNNNAGG 1 cut(s) 2243
BstHHI GCGC 1 cut(s) 3177
BstMAI GTCTC 6 cut(s) 94, 338, 385, 1811, 2252, 2471
BstMCI CGRYCG 1 cut(s) 340
BstNI CCWGG 3 cut(s) 629, 1098, 2281
BstNSI RCATGY 1 cut(s) 3157
BstPAI GACNNNNGTC 1 cut(s) 762
BstSCI CCNGG 4 cut(s) 627, 1096, 1710, 2279
BstSFI CTRYAG 4 cut(s) 633, 1538, 1796, 2791
BstSLI GKGCMC 4 cut(s) 535, 1588, 2051, 2287
BstV2I GAAGAC 1 cut(s) 516
BstX2I RGATCY 4 cut(s) 307, 799, 1211, 3303
BstYI RGATCY 4 cut(s) 307, 799, 1211, 3303
BstZI CGGCCG 1 cut(s) 337
Bsu15I ATCGAT 1 cut(s) 2628
Bsu36I CCTNAGG 1 cut(s) 2346
BsuI GTATCC 1 cut(s) 2807
BsuTUI ATCGAT 1 cut(s) 2628
BtgI CCRYGG 3 cut(s) 916, 1347, 2979
BtgZI GCGATG 3 cut(s) 60, 1927, 2815
BtsI GCAGTG 6 cut(s) 202, 751, 1752, 1793, 2080, 2865
Cac8I GCNNGC 6 cut(s) 320, 327, 1731, 1810, 2037, 3265
CaiI CAGNNNCTG 3 cut(s) 201, 1748, 1820
CfoI GCGC 1 cut(s) 3177
Cfr10I RCCGGY 4 cut(s) 318, 431, 2617, 2834
ClaI ATCGAT 1 cut(s) 2628
CseI GACGC 2 cut(s) 241, 1203
CsiI ACCWGGT 1 cut(s) 1096
Csp6I GTAC 7 cut(s) 334, 1950, 2170, 2450, 2552, 2902, 2993
CspCI CAANNNNNGTGG 2 cut(s) 2854, 2889
CviAII CATG 9 cut(s) 268, 455, 855, 917, 1348, 2091, 2980, 3154, 3178
CviQI GTAC 7 cut(s) 334, 1950, 2170, 2450, 2552, 2902, 2993
EaeI YGGCCR 3 cut(s) 337, 1136, 2913
EagI CGGCCG 1 cut(s) 337
Eam1104I CTCTTC 3 cut(s) 1676, 1712, 2970
EarI CTCTTC 3 cut(s) 1676, 1712, 2970
EciI GGCGGA 2 cut(s) 126, 3236
EclXI CGGCCG 1 cut(s) 337
Eco130I CCWWGG 7 cut(s) 397, 916, 1053, 1347, 1467, 1954, 2979
Eco147I AGGCCT 2 cut(s) 396, 1394
Eco24I GRGCYC 3 cut(s) 535, 2098, 2287
Eco32I GATATC 2 cut(s) 649, 852
Eco47I GGWCC 6 cut(s) 481, 764, 1471, 2020, 2128, 2370
Eco52I CGGCCG 1 cut(s) 337
Eco57I CTGAAG 6 cut(s) 528, 744, 930, 2157, 2823, 2837
Eco81I CCTNAGG 1 cut(s) 2346
EcoNI CCTNNNNNAGG 1 cut(s) 2243
EcoO109I RGGNCCY 6 cut(s) 481, 532, 733, 2020, 2128, 2370
EcoRI GAATTC 2 cut(s) 286, 671
EcoRII CCWGG 3 cut(s) 627, 1096, 2279
EcoRV GATATC 2 cut(s) 649, 852
EcoT14I CCWWGG 7 cut(s) 397, 916, 1053, 1347, 1467, 1954, 2979
EcoT38I GRGCYC 3 cut(s) 535, 2098, 2287
ErhI CCWWGG 7 cut(s) 397, 916, 1053, 1347, 1467, 1954, 2979
Esp3I CGTCTC 1 cut(s) 338
FaeI CATG 9 cut(s) 271, 458, 858, 920, 1351, 2094, 2983, 3157, 3181
FalI AAGNNNNNCTT 2 cut(s) 600, 632
FaqI GGGAC 5 cut(s) 494, 2033, 2114, 2383, 3053
FatI CATG 9 cut(s) 267, 454, 854, 916, 1347, 2090, 2979, 3153, 3177
FauNDI CATATG 2 cut(s) 2748, 3189
FbaI TGATCA 2 cut(s) 1294, 1906
FblI GTMKAC 3 cut(s) 1696, 2513, 2808
FriOI GRGCYC 3 cut(s) 535, 2098, 2287
GlaI GCGC 1 cut(s) 3176
GsaI CCCAGC 1 cut(s) 2135
GsuI CTGGAG 1 cut(s) 1571
HapII CCGG 6 cut(s) 319, 432, 1711, 2618, 2835, 2916
HgaI GACGC 2 cut(s) 241, 1203
HhaI GCGC 1 cut(s) 3177
Hin1II CATG 9 cut(s) 271, 458, 858, 920, 1351, 2094, 2983, 3157, 3181
Hin6I GCGC 1 cut(s) 3175
HinP1I GCGC 1 cut(s) 3175
HincII GTYRAC 3 cut(s) 2331, 2539, 2809
HindII GTYRAC 3 cut(s) 2331, 2539, 2809
HindIII AAGCTT 1 cut(s) 170
HpaI GTTAAC 2 cut(s) 2331, 2539
HpaII CCGG 6 cut(s) 319, 432, 1711, 2618, 2835, 2916
HphI GGTGA 4 cut(s) 806, 1112, 1403, 2207
Hpy188I TCNGA 9 cut(s) 622, 1113, 1186, 1216, 1299, 1458, 2104, 3240, 3303
Hpy99I CGWCG 1 cut(s) 185
HpyCH4III ACNGT 9 cut(s) 242, 778, 1499, 1573, 1744, 1775, 2053, 2318, 3034
HpyCH4IV ACGT 3 cut(s) 61, 1827, 2544
HpySE526I ACGT 3 cut(s) 61, 1827, 2544
Hsp92II CATG 9 cut(s) 271, 458, 858, 920, 1351, 2094, 2983, 3157, 3181
HspAI GCGC 1 cut(s) 3175
KflI GGGWCCC 2 cut(s) 2020, 2370
KpnI GGTACC 2 cut(s) 1953, 2555
KroI GCCGGC 1 cut(s) 318
KroNI GCCGGC 1 cut(s) 320
Ksp22I TGATCA 2 cut(s) 1294, 1906
KspAI GTTAAC 2 cut(s) 2331, 2539
LguI GCTCTTC 1 cut(s) 2970
LmnI GCTCC 8 cut(s) 1529, 2009, 2392, 2680, 2691, 2896, 3084, 3223
MabI ACCWGGT 1 cut(s) 1096
MaeII ACGT 3 cut(s) 61, 1827, 2544
MaeIII GTNAC 8 cut(s) 197, 215, 445, 755, 1033, 1246, 1310, 1685
MfeI CAATTG 3 cut(s) 666, 984, 2853
MflI RGATCY 4 cut(s) 307, 799, 1211, 3303
MhlI GDGCHC 7 cut(s) 535, 579, 1588, 2051, 2098, 2287, 2925
MmeI TCCRAC 4 cut(s) 393, 1354, 1481, 2337
MroNI GCCGGC 1 cut(s) 318
MroXI GAANNNNTTC 1 cut(s) 1452
MslI CAYNNNNRTG 5 cut(s) 1591, 1976, 2044, 2751, 3249
MspA1I CMGCKG 2 cut(s) 601, 2135
MspI CCGG 6 cut(s) 319, 432, 1711, 2618, 2835, 2916
MspR9I CCNGG 4 cut(s) 629, 1098, 1712, 2281
MunI CAATTG 3 cut(s) 666, 984, 2853
Mva1269I GAATGC 3 cut(s) 967, 2194, 2240
MvaI CCWGG 3 cut(s) 629, 1098, 2281
NaeI GCCGGC 1 cut(s) 320
NciI CCSGG 1 cut(s) 1712
NcoI CCATGG 3 cut(s) 916, 1347, 2979
NdeI CATATG 2 cut(s) 2748, 3189
NgoMIV GCCGGC 1 cut(s) 318
NlaIII CATG 9 cut(s) 271, 458, 858, 920, 1351, 2094, 2983, 3157, 3181
NmeAIII GCCGAG 4 cut(s) 24, 430, 972, 1247
NmuCI GTSAC 4 cut(s) 197, 445, 755, 1310
NspI RCATGY 1 cut(s) 3157
OliI CACNNNNGTG 1 cut(s) 2044
PceI AGGCCT 2 cut(s) 396, 1394
PciSI GCTCTTC 1 cut(s) 2970
PctI GAATGC 3 cut(s) 967, 2194, 2240
PdiI GCCGGC 1 cut(s) 320
PdmI GAANNNNTTC 1 cut(s) 1452
PfoI TCCNGGA 1 cut(s) 1710
PpuMI RGGWCCY 4 cut(s) 481, 2020, 2128, 2370
PshAI GACNNNNGTC 1 cut(s) 762
PsiI TTATAA 2 cut(s) 1769, 3053
Psp5II RGGWCCY 4 cut(s) 481, 2020, 2128, 2370
Psp6I CCWGG 3 cut(s) 627, 1096, 2279
PspFI CCCAGC 1 cut(s) 2131
PspGI CCWGG 3 cut(s) 627, 1096, 2279
PspOMI GGGCCC 2 cut(s) 531, 2283
PspPPI RGGWCCY 4 cut(s) 481, 2020, 2128, 2370
PstI CTGCAG 2 cut(s) 1542, 1800
PstNI CAGNNNCTG 3 cut(s) 201, 1748, 1820
PsuI RGATCY 4 cut(s) 307, 799, 1211, 3303
PvuII CAGCTG 2 cut(s) 601, 2135
RsaI GTAC 7 cut(s) 335, 1951, 2171, 2451, 2553, 2903, 2994
RsaNI GTAC 7 cut(s) 334, 1950, 2170, 2450, 2552, 2902, 2993
RseI CAYNNNNRTG 5 cut(s) 1591, 1976, 2044, 2751, 3249
SalI GTCGAC 1 cut(s) 2807
SapI GCTCTTC 1 cut(s) 2970
ScrFI CCNGG 4 cut(s) 629, 1098, 1712, 2281
SduI GDGCHC 7 cut(s) 535, 579, 1588, 2051, 2098, 2287, 2925
SexAI ACCWGGT 1 cut(s) 1096
SfcI CTRYAG 4 cut(s) 633, 1538, 1796, 2791
SinI GGWCC 6 cut(s) 481, 764, 1471, 2020, 2128, 2370
SmiMI CAYNNNNRTG 5 cut(s) 1591, 1976, 2044, 2751, 3249
SmlI CTYRAG 3 cut(s) 875, 1283, 2120
SmoI CTYRAG 3 cut(s) 875, 1283, 2120
SseBI AGGCCT 2 cut(s) 396, 1394
SsiI CCGC 7 cut(s) 34, 37, 57, 111, 260, 355, 3221
SspI AATATT 2 cut(s) 26, 1786
StuI AGGCCT 2 cut(s) 396, 1394
StyD4I CCNGG 4 cut(s) 627, 1096, 1710, 2279
StyI CCWWGG 7 cut(s) 397, 916, 1053, 1347, 1467, 1954, 2979
TaaI ACNGT 9 cut(s) 242, 778, 1499, 1573, 1744, 1775, 2053, 2318, 3034
TaiI ACGT 3 cut(s) 64, 1830, 2547
TaqI TCGA 6 cut(s) 584, 675, 861, 1437, 2628, 2808
TaqII GACCGA 1 cut(s) 941
TatI WGTACW 3 cut(s) 2169, 2449, 2901
TauI GCSGC 3 cut(s) 39, 262, 358
TseFI GTSAC 4 cut(s) 197, 445, 755, 1310
Tsp45I GTSAC 4 cut(s) 197, 445, 755, 1310
TspDTI ATGAA 9 cut(s) 474, 966, 1225, 2045, 2049, 2198, 2675, 2720, 2818
TspGWI ACGGA 1 cut(s) 954
VneI GTGCAC 2 cut(s) 1584, 2047
VpaK11BI GGWCC 6 cut(s) 481, 764, 1471, 2020, 2128, 2370
XagI CCTNNNNNAGG 1 cut(s) 2243
XapI RAATTY 8 cut(s) 286, 671, 1064, 1515, 1609, 1852, 2558, 2825
XbaI TCTAGA 1 cut(s) 1876
XceI RCATGY 1 cut(s) 3157
XmaJI CCTAGG 1 cut(s) 1954
XmiI GTMKAC 3 cut(s) 1696, 2513, 2808
XmnI GAANNNNTTC 1 cut(s) 1452
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.