Rmu_sc0006845.1_g000008

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0006845.1
OG0003792
Physical Location & Seq
Forward (+)
49657 .. 52594
2938 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1659 bp
atgggtgaatcaatagtgggttttccaaaagatgctgataagatgggagagatagctggtacagattcatcaaatccttctctggaagtgtcggtttcatttggtaggtttgagaatgattcattatcttgggagaaatggtcggcattttcgccaaataagtacttggaagaagttgagaagtgtgctactcctggttcggtagctcagaaaagggcttattttgaagctcattacaagaggatagctgctcgaaaagccgaggagcttctggaacaagagaagcaaatgcaagatgaggatgatcccttaaagtcagatgatcaaaataatggagatcagatctgttgtggtacggattgtggaattgatgtagctaccagtcaaactagtgctcaagcaaatagtcaagagcccaatatagagaatgggatgagcagcactcatgtggaagatctcaaggaggaggatgaggatgttttcacaatggaatgtcaaacatcatcgattgagggtagagaaatagaggaaactgatagcggagtggttagtccgaagactccaaagctgaacagaccagaagaacttgttttggtaaaagaagtggaaaccatcactgctgacactcaagaaacaatacaggaagttccaaagactttggacagtgatacaggcaatgctccagaagttaaagaggaaaaagcgagattcgatcctcagaagagatctcagaaggttactccagtgaacaaggagaggacagtggcaaaggccaagaagaaatcaatttcaccaatgactaaaacacctcagaatccgacccctagactgtcaaagttaccacagaattccacccctagagtgtcaaagttaccacagaattccacccctagagtgtcaaagataccgcagaatactacccctagagtgtcaaagatactgcagaataccacccctagtgtgtcaaagccaatatcagctcctaacggagcaaaatctgcaccaagatcctcagtaacgaaggccaatggttcatctttaactaggagcacaaatcagtcaagtgaaaaaaccaagaaagtgcctcctaaatctctgcacatgtcactcagtttcaatcctaaaaagtctgattcagcttcagaaactgtggttacagctagaaaatctttgattatggagcaaatgggtgacaaggacattgtcaaacgagcattcaagacatttcaaaacagtgtcaaccaattgaaatcctctaatgaagagaaaccttcagcaccaatgcagctccctacaaagtcgaaagaaccaaaggtttccactcctatgcctctgccgaaagacactggagggtcactcaaaacgagttatccggataaaagaaatgccaaggctgccccatcatcttttggcctgagaagtgaagaaagagcagagaaacggaaggagttaacaaacaacttggcgaaacccaatgccagagatgcagagagagcacacttccagccaaagtcaaaggagcaaaaggaggcagagataaaaaagctaagacagagtcttaagttcaaggctacaccaacgcctgattcttatcggggacaaaagttatcgaaaagtactctagaaaaggagggtcctaagaaagaaacgcttcggtga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TPX2

Protein Analysis

552

Amino Acids

60.98

Weight (kDa)

9.16

Isoelectric Point (pI)

47.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TPX2 PF06886 470 - 539 7.1e-13 Targeting protein for Xklp2 (TPX2) domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 1372
AciI CCGC 2 cut(s) 540, 908
AclWI GGATC 3 cut(s) 299, 707, 1002
AcsI RAATTY 2 cut(s) 847, 880
AcuI CTGAAG 2 cut(s) 1125, 1257
AfaI GTAC 4 cut(s) 61, 164, 355, 1618
AflII CTTAAG 1 cut(s) 1559
AflIII ACRYGT 1 cut(s) 1101
AgsI TTSAA 6 cut(s) 227, 1117, 1219, 1229, 1249, 1567
AhlI ACTAGT 1 cut(s) 389
AjnI CCWGG 1 cut(s) 193
AjuI GAANNNNNNNTTGG 2 cut(s) 1570, 1602
AleI CACNNNNGTG 1 cut(s) 446
Alw21I GWGCWC 3 cut(s) 397, 1052, 1498
AlwI GGATC 3 cut(s) 299, 707, 1002
AlwNI CAGNNNCTG 1 cut(s) 1148
Aor13HI TCCGGA 1 cut(s) 1372
AoxI GGCC 3 cut(s) 771, 1023, 1411
ApeKI GCWGC 4 cut(s) 248, 438, 1285, 1394
ApoI RAATTY 2 cut(s) 847, 880
Asp700I GAANNNNTTC 1 cut(s) 1650
AspS9I GGNCC 1 cut(s) 1634
AsuHPI GGTGA 3 cut(s) 17, 783, 1202
AvaII GGWCC 1 cut(s) 1634
BanII GRGCYC 1 cut(s) 417
BbsI GAAGAC 1 cut(s) 563
Bbv12I GWGCWC 3 cut(s) 397, 1052, 1498
BbvI GCAGC 4 cut(s) 235, 450, 1297, 1381
BccI CCATC 3 cut(s) 37, 620, 1408
BciT130I CCWGG 1 cut(s) 195
BclI TGATCA 1 cut(s) 322
BcuI ACTAGT 1 cut(s) 389
BfaI CTAG 9 cut(s) 390, 825, 858, 891, 924, 957, 1044, 1161, 1622
BfmI CTRYAG 1 cut(s) 941
BfrI CTTAAG 1 cut(s) 1559
BglII AGATCT 3 cut(s) 342, 454, 725
BisI GCNGC 4 cut(s) 249, 439, 1286, 1395
BlsI GCNGC 4 cut(s) 250, 440, 1287, 1396
BmcAI AGTACT 2 cut(s) 164, 1618
Bme1390I CCNGG 1 cut(s) 195
Bme18I GGWCC 1 cut(s) 1634
BmgT120I GGNCC 1 cut(s) 1634
BmiI GGNNCC 1 cut(s) 1635
BmrFI CCNGG 1 cut(s) 195
BmsI GCATC 2 cut(s) 22, 1474
BpiI GAAGAC 1 cut(s) 563
BplI GAGNNNNNCTC 4 cut(s) 427, 459, 1341, 1373
BpmI CTGGAG 3 cut(s) 666, 726, 1368
BpuEI CTTGAG 3 cut(s) 381, 443, 612
Bsa29I ATCGAT 1 cut(s) 506
BsaBI GATNNNNATC 1 cut(s) 1590
BsaJI CCNNGG 2 cut(s) 261, 1389
BsaWI WCCGGW 1 cut(s) 1372
BsaXI ACNNNNNCTCC 2 cut(s) 125, 155
Bse1I ACTGG 3 cut(s) 381, 743, 1351
Bse3DI GCAATG 1 cut(s) 682
Bse8I GATNNNNATC 1 cut(s) 1590
BseAI TCCGGA 1 cut(s) 1372
BseBI CCWGG 1 cut(s) 195
BseCI ATCGAT 1 cut(s) 506
BseDI CCNNGG 2 cut(s) 261, 1389
BseGI GGATG 4 cut(s) 307, 438, 475, 481
BseJI GATNNNNATC 1 cut(s) 1590
BseMI GCAATG 1 cut(s) 682
BseMII CTCAG 7 cut(s) 221, 731, 743, 824, 1026, 1123, 1406
BseNI ACTGG 3 cut(s) 381, 743, 1351
BseRI GAGGAG 2 cut(s) 278, 479
BseXI GCAGC 4 cut(s) 235, 450, 1297, 1381
BsgI GTGCAG 2 cut(s) 984, 1082
BshFI GGCC 3 cut(s) 773, 1025, 1413
BshVI ATCGAT 1 cut(s) 506
BsiHKAI GWGCWC 3 cut(s) 397, 1052, 1498
BsiSI CCGG 1 cut(s) 1373
BslFI GGGAC 1 cut(s) 1611
BsmFI GGGAC 1 cut(s) 1611
BsmI GAATGC 1 cut(s) 1214
BsnI GGCC 3 cut(s) 773, 1025, 1413
Bsp1286I GDGCHC 4 cut(s) 397, 417, 1052, 1498
Bsp13I TCCGGA 1 cut(s) 1372
Bsp143I GATC 8 cut(s) 304, 322, 337, 342, 454, 712, 725, 1007
BspACI CCGC 2 cut(s) 540, 908
BspANI GGCC 3 cut(s) 773, 1025, 1413
BspCNI CTCAG 7 cut(s) 220, 730, 742, 823, 1025, 1122, 1407
BspDI ATCGAT 1 cut(s) 506
BspEI TCCGGA 1 cut(s) 1372
BspLI GGNNCC 1 cut(s) 1635
BspMAI CTGCAG 1 cut(s) 945
BspPI GGATC 3 cut(s) 299, 707, 1002
BspTI CTTAAG 1 cut(s) 1559
BsrDI GCAATG 1 cut(s) 682
BsrI ACTGG 3 cut(s) 381, 743, 1351
BssECI CCNNGG 2 cut(s) 261, 1389
BssMI GATC 8 cut(s) 304, 322, 337, 342, 454, 712, 725, 1007
BssT1I CCWWGG 1 cut(s) 1389
Bst2UI CCWGG 1 cut(s) 195
Bst4CI ACNGT 5 cut(s) 665, 763, 831, 1150, 1235
Bst6I CTCTTC 2 cut(s) 716, 1257
BstAFI CTTAAG 1 cut(s) 1559
BstAPI GCANNNNNTGC 1 cut(s) 998
BstDEI CTNAG 9 cut(s) 207, 717, 729, 810, 1012, 1109, 1415, 1547, 1638
BstF5I GGATG 4 cut(s) 307, 438, 475, 481
BstKTI GATC 8 cut(s) 307, 325, 340, 345, 457, 715, 728, 1010
BstMBI GATC 8 cut(s) 304, 322, 337, 342, 454, 712, 725, 1007
BstMWI GCNNNNNNNGC 5 cut(s) 257, 998, 1394, 1484, 1493
BstNI CCWGG 1 cut(s) 195
BstNSI RCATGY 1 cut(s) 1105
BstSCI CCNGG 1 cut(s) 193
BstSFI CTRYAG 1 cut(s) 941
BstV1I GCAGC 4 cut(s) 235, 450, 1297, 1381
BstV2I GAAGAC 1 cut(s) 563
BstX2I RGATCY 4 cut(s) 342, 454, 725, 1007
BstYI RGATCY 4 cut(s) 342, 454, 725, 1007
Bsu15I ATCGAT 1 cut(s) 506
BsuRI GGCC 3 cut(s) 773, 1025, 1413
BsuTUI ATCGAT 1 cut(s) 506
BtsCI GGATG 4 cut(s) 307, 438, 475, 481
BtsI GCAGTG 1 cut(s) 615
BtsIMutI CAGTG 6 cut(s) 615, 670, 750, 768, 1240, 1344
CaiI CAGNNNCTG 1 cut(s) 1148
Cfr13I GGNCC 1 cut(s) 1634
ClaI ATCGAT 1 cut(s) 506
Csp6I GTAC 4 cut(s) 60, 163, 354, 1617
CviAII CATG 2 cut(s) 446, 1102
CviQI GTAC 4 cut(s) 60, 163, 354, 1617
DdeI CTNAG 9 cut(s) 207, 717, 729, 810, 1012, 1109, 1415, 1547, 1638
DpnI GATC 8 cut(s) 306, 324, 339, 344, 456, 714, 727, 1009
DpnII GATC 8 cut(s) 304, 322, 337, 342, 454, 712, 725, 1007
Eam1104I CTCTTC 2 cut(s) 716, 1257
EarI CTCTTC 2 cut(s) 716, 1257
Eco130I CCWWGG 1 cut(s) 1389
Eco24I GRGCYC 1 cut(s) 417
Eco47I GGWCC 1 cut(s) 1634
Eco57I CTGAAG 2 cut(s) 1125, 1257
EcoO109I RGGNCCY 1 cut(s) 1634
EcoRI GAATTC 2 cut(s) 847, 880
EcoRII CCWGG 1 cut(s) 193
EcoT14I CCWWGG 1 cut(s) 1389
EcoT38I GRGCYC 1 cut(s) 417
ErhI CCWWGG 1 cut(s) 1389
FaeI CATG 2 cut(s) 449, 1105
FaiI YATR 5 cut(s) 422, 447, 1103, 1178, 1328
FalI AAGNNNNNCTT 1 cut(s) 1635
FaqI GGGAC 1 cut(s) 1611
FatI CATG 2 cut(s) 445, 1101
FbaI TGATCA 1 cut(s) 322
Fnu4HI GCNGC 4 cut(s) 249, 439, 1286, 1395
FokI GGATG 4 cut(s) 314, 445, 482, 488
FriOI GRGCYC 1 cut(s) 417
Fsp4HI GCNGC 4 cut(s) 249, 439, 1286, 1395
FspBI CTAG 9 cut(s) 390, 825, 858, 891, 924, 957, 1044, 1161, 1622
GluI GCNGC 4 cut(s) 249, 439, 1286, 1395
GsuI CTGGAG 3 cut(s) 666, 726, 1368
HaeIII GGCC 3 cut(s) 773, 1025, 1413
HapII CCGG 1 cut(s) 1373
Hin1II CATG 2 cut(s) 449, 1105
HincII GTYRAC 2 cut(s) 1240, 1452
HindII GTYRAC 2 cut(s) 1240, 1452
HinfI GANTC 9 cut(s) 8, 65, 119, 559, 708, 814, 1133, 1555, 1586
HpaI GTTAAC 1 cut(s) 1452
HpaII CCGG 1 cut(s) 1373
HphI GGTGA 3 cut(s) 17, 783, 1202
Hpy166II GTNNAC 3 cut(s) 748, 1240, 1452
Hpy188III TCNNGA 8 cut(s) 83, 272, 410, 629, 683, 1219, 1373, 1622
Hpy8I GTNNAC 3 cut(s) 748, 1240, 1452
HpyAV CCTTC 5 cut(s) 87, 727, 1015, 1281, 1438
HpyCH4III ACNGT 5 cut(s) 665, 763, 831, 1150, 1235
HpyCH4V TGCA 6 cut(s) 292, 943, 1001, 1099, 1285, 1487
HpyF10VI GCNNNNNNNGC 5 cut(s) 257, 998, 1394, 1484, 1493
HpyF3I CTNAG 9 cut(s) 207, 717, 729, 810, 1012, 1109, 1415, 1547, 1638
Hsp92II CATG 2 cut(s) 449, 1105
Kpn2I TCCGGA 1 cut(s) 1372
Ksp22I TGATCA 1 cut(s) 322
KspAI GTTAAC 1 cut(s) 1452
Kzo9I GATC 8 cut(s) 304, 322, 337, 342, 454, 712, 725, 1007
LmnI GCTCC 8 cut(s) 265, 685, 985, 989, 1047, 1180, 1293, 1519
Lsp1109I GCAGC 4 cut(s) 235, 450, 1297, 1381
LweI GCATC 2 cut(s) 22, 1474
MaeI CTAG 9 cut(s) 390, 825, 858, 891, 924, 957, 1044, 1161, 1622
MaeIII GTNAC 8 cut(s) 736, 837, 870, 1015, 1104, 1153, 1190, 1353
MalI GATC 8 cut(s) 306, 324, 339, 344, 456, 714, 727, 1009
MboI GATC 8 cut(s) 304, 322, 337, 342, 454, 712, 725, 1007
MboII GAAGA 8 cut(s) 182, 464, 568, 593, 733, 790, 1274, 1436
MfeI CAATTG 1 cut(s) 1244
MflI RGATCY 4 cut(s) 342, 454, 725, 1007
MhlI GDGCHC 4 cut(s) 397, 417, 1052, 1498
MluCI AATT 5 cut(s) 366, 786, 847, 880, 1244
MlyI GAGTC 2 cut(s) 553, 1564
MmeI TCCRAC 1 cut(s) 842
MroI TCCGGA 1 cut(s) 1372
MroXI GAANNNNTTC 1 cut(s) 1650
MseI TTAA 5 cut(s) 311, 690, 1040, 1451, 1560
MslI CAYNNNNRTG 2 cut(s) 446, 1325
MspCI CTTAAG 1 cut(s) 1559
MspI CCGG 1 cut(s) 1373
MspR9I CCNGG 1 cut(s) 195
MunI CAATTG 1 cut(s) 1244
Mva1269I GAATGC 1 cut(s) 1214
MvaI CCWGG 1 cut(s) 195
MwoI GCNNNNNNNGC 5 cut(s) 257, 998, 1394, 1484, 1493
NdeII GATC 8 cut(s) 304, 322, 337, 342, 454, 712, 725, 1007
NlaIII CATG 2 cut(s) 449, 1105
NlaIV GGNNCC 1 cut(s) 1635
NmeAIII GCCGAG 1 cut(s) 286
NmuCI GTSAC 3 cut(s) 1104, 1190, 1353
NspI RCATGY 1 cut(s) 1105
OliI CACNNNNGTG 1 cut(s) 446
PciI ACATGT 1 cut(s) 1101
PctI GAATGC 1 cut(s) 1214
PdmI GAANNNNTTC 1 cut(s) 1650
PfeI GAWTC 7 cut(s) 8, 65, 119, 708, 814, 1133, 1586
PflFI GACNNNGTC 2 cut(s) 1202, 1554
PkrI GCNGC 4 cut(s) 250, 440, 1287, 1396
PleI GAGTC 2 cut(s) 553, 1563
PpsI GAGTC 2 cut(s) 553, 1563
PpuMI RGGWCCY 1 cut(s) 1634
PscI ACATGT 1 cut(s) 1101
Psp5II RGGWCCY 1 cut(s) 1634
Psp6I CCWGG 1 cut(s) 193
PspGI CCWGG 1 cut(s) 193
PspN4I GGNNCC 1 cut(s) 1635
PspPI GGNCC 1 cut(s) 1634
PspPPI RGGWCCY 1 cut(s) 1634
PstI CTGCAG 1 cut(s) 945
PstNI CAGNNNCTG 1 cut(s) 1148
PsuI RGATCY 4 cut(s) 342, 454, 725, 1007
PsyI GACNNNGTC 2 cut(s) 1202, 1554
RsaI GTAC 4 cut(s) 61, 164, 355, 1618
RsaNI GTAC 4 cut(s) 60, 163, 354, 1617
RseI CAYNNNNRTG 2 cut(s) 446, 1325
SaqAI TTAA 5 cut(s) 311, 690, 1040, 1451, 1560
SatI GCNGC 4 cut(s) 249, 439, 1286, 1395
Sau3AI GATC 8 cut(s) 304, 322, 337, 342, 454, 712, 725, 1007
Sau96I GGNCC 1 cut(s) 1634
ScaI AGTACT 2 cut(s) 164, 1618
SchI GAGTC 2 cut(s) 553, 1564
ScrFI CCNGG 1 cut(s) 195
SduI GDGCHC 4 cut(s) 397, 417, 1052, 1498
SfaNI GCATC 2 cut(s) 22, 1474
SfcI CTRYAG 1 cut(s) 941
SinI GGWCC 1 cut(s) 1634
SmiMI CAYNNNNRTG 2 cut(s) 446, 1325
SmlI CTYRAG 4 cut(s) 396, 458, 627, 1559
SmoI CTYRAG 4 cut(s) 396, 458, 627, 1559
SpeI ACTAGT 1 cut(s) 389
Sse9I AATT 5 cut(s) 366, 786, 847, 880, 1244
SsiI CCGC 2 cut(s) 540, 908
SspMI CTAG 9 cut(s) 390, 825, 858, 891, 924, 957, 1044, 1161, 1622
StyD4I CCNGG 1 cut(s) 193
StyI CCWWGG 1 cut(s) 1389
TaaI ACNGT 5 cut(s) 665, 763, 831, 1150, 1235
TaqI TCGA 5 cut(s) 253, 506, 711, 1301, 1610
TasI AATT 5 cut(s) 366, 786, 847, 880, 1244
TatI WGTACW 2 cut(s) 162, 1616
TfiI GAWTC 7 cut(s) 8, 65, 119, 708, 814, 1133, 1586
Tru1I TTAA 5 cut(s) 311, 690, 1040, 1451, 1560
Tru9I TTAA 5 cut(s) 311, 690, 1040, 1451, 1560
TscAI CASTG 6 cut(s) 622, 670, 750, 768, 1240, 1351
TseFI GTSAC 3 cut(s) 1104, 1190, 1353
TseI GCWGC 4 cut(s) 248, 438, 1285, 1394
Tsp45I GTSAC 3 cut(s) 1104, 1190, 1353
TspDTI ATGAA 5 cut(s) 57, 87, 111, 1023, 1275
TspGWI ACGGA 3 cut(s) 371, 1002, 1456
TspRI CASTG 6 cut(s) 622, 670, 750, 768, 1240, 1351
Tth111I GACNNNGTC 2 cut(s) 1202, 1554
Vha464I CTTAAG 1 cut(s) 1559
VpaK11BI GGWCC 1 cut(s) 1634
XapI RAATTY 2 cut(s) 847, 880
XbaI TCTAGA 1 cut(s) 1621
XceI RCATGY 1 cut(s) 1105
XmnI GAANNNNTTC 1 cut(s) 1650
XspI CTAG 9 cut(s) 390, 825, 858, 891, 924, 957, 1044, 1161, 1622
ZrmI AGTACT 2 cut(s) 164, 1618
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.