Rmu_sc0007121.1_g000029

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0007121.1
OG0013763
Physical Location & Seq
Reverse (-)
106857 .. 108154
1298 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1206 bp
atgaaatccttgaaaaggttggatctaagccgtactgcaatcagagaattgccttcatcaacaattagatgtctcattaatctggaagagttaactttatcatactgccataaacttaaacatgtgccgtgcagcatttttgagttgcaacatctagagcttttggatctctcttattgtaaaaagctggtaacatttccaacaaagtcagggttttcaacgggatcgaccagtttacaggacaagcactatgttccattattcgtcaatttgaacaattgcaatcgtctcgtagaaattgcagaaattccgcgagaaagatatgacctagatgcgagtggctgctatggattgcaaaaaatttcaaaattgtcaaacattttggaaggtaaacagtcaaaattgaccccttttatggacttgcttaagtggtatgaactgtccggcaatctggctcgtgacgtggcaaaggtgaaaaagaatttaccggataattcctggtcaacagctctgtggtttctcgttttctcatgtcggcaatctgagtttcaggttttctttaacggcccgccttacaaatttgggattccggagtggttcacttggcgcagggatttcgatatggaaggacttcaaatggaaggacttcaaatggaaggatgtgaatttagaattgattttcctggaaatttcaaatgggaggacaaaggattggcgttctgtgctcaaactgttgataacgatcaccagcgctgggctatgagatcatgtttcagaatagtcacaatctacgttaacgctgtatgcatcactgaagtatacctaatgacgggatggtccgtgtggggatacttgccattcgatactattatagaacggttgagtgagagtgggtcaccaccaccttccgtgtgtctggttaagattgaatttaaaacccgcgatgtatggtctaacgagattaaggacgaagggcccccgatgcaaagctgcggagtccacgttgtaatgccagaggatgaaggccaatttgttttgagcttaccgaccccttcagtcaaacaaggcaagccatacctccaccacatatggagtaatgtttatcctgtggacgaatttcgccacatttatgaagaacgactgccatctaacgctgtgaaaacagagcagatgtggaatgctatccacatggacatcatggagtag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NB-ARC TIR

Protein Analysis

401

Amino Acids

46.52

Weight (kDa)

5.99

Isoelectric Point (pI)

47.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_8 PF13855 3 - 60 4.3e-06 Leucine rich repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 835
AccI GTMKAC 1 cut(s) 819
AccII CGCG 2 cut(s) 313, 942
AccIII TCCGGA 1 cut(s) 589
AciI CCGC 4 cut(s) 311, 569, 940, 993
AclWI GGATC 3 cut(s) 30, 174, 232
AcsI RAATTY 8 cut(s) 306, 360, 481, 578, 665, 688, 929, 1115
AcuI CTGAAG 2 cut(s) 834, 1038
AfaI GTAC 1 cut(s) 34
AfeI AGCGCT 1 cut(s) 752
AfiI CCNNNNNNNGG 4 cut(s) 15, 415, 754, 829
AflII CTTAAG 1 cut(s) 425
AflIII ACRYGT 1 cut(s) 121
AgsI TTSAA 8 cut(s) 13, 219, 274, 366, 635, 650, 694, 929
AjiI CACGTC 1 cut(s) 463
AjnI CCWGG 2 cut(s) 497, 682
AluBI AGCT 5 cut(s) 160, 187, 509, 990, 1041
AluI AGCT 5 cut(s) 160, 187, 509, 990, 1041
Alw21I GWGCWC 1 cut(s) 727
Alw26I GTCTC 2 cut(s) 77, 293
AlwI GGATC 3 cut(s) 30, 174, 232
Aor13HI TCCGGA 1 cut(s) 589
Aor51HI AGCGCT 1 cut(s) 752
AoxI GGCC 3 cut(s) 565, 974, 1024
ApaI GGGCCC 1 cut(s) 978
ApeKI GCWGC 3 cut(s) 132, 342, 990
ApoI RAATTY 8 cut(s) 306, 360, 481, 578, 665, 688, 929, 1115
AseI ATTAAT 1 cut(s) 78
Asp700I GAANNNNTTC 2 cut(s) 630, 645
AspLEI GCGC 2 cut(s) 609, 753
AspS9I GGNCC 4 cut(s) 566, 837, 974, 975
AsuHPI GGTGA 3 cut(s) 484, 737, 888
AvaII GGWCC 1 cut(s) 837
BaeGI GKGCMC 1 cut(s) 978
BanII GRGCYC 1 cut(s) 978
BauI CACGAG 1 cut(s) 456
Bbv12I GWGCWC 1 cut(s) 727
BbvI GCAGC 3 cut(s) 144, 329, 977
BccI CCATC 2 cut(s) 828, 1153
BceAI ACGGC 3 cut(s) 15, 112, 580
BcgI CGANNNNNNTGC 4 cut(s) 271, 305, 598, 632
BciT130I CCWGG 2 cut(s) 499, 684
BciVI GTATCC 1 cut(s) 842
BcoDI GTCTC 2 cut(s) 77, 293
BfaI CTAG 2 cut(s) 155, 329
BfoI RGCGCY 1 cut(s) 754
BfrI CTTAAG 1 cut(s) 425
BfuI GTATCC 1 cut(s) 842
BisI GCNGC 3 cut(s) 133, 343, 991
BlsI GCNGC 3 cut(s) 134, 344, 992
Bme1390I CCNGG 2 cut(s) 499, 684
Bme18I GGWCC 1 cut(s) 837
BmgBI CACGTC 1 cut(s) 463
BmgT120I GGNCC 4 cut(s) 566, 837, 974, 975
BmiI GGNNCC 2 cut(s) 976, 977
BmrFI CCNGG 2 cut(s) 499, 684
BmsI GCATC 3 cut(s) 322, 816, 972
BsaBI GATNNNNATC 1 cut(s) 741
BsaWI WCCGGW 2 cut(s) 487, 589
BsaXI ACNNNNNCTCC 2 cut(s) 987, 1017
Bsc4I CCNNNNNNNGG 4 cut(s) 15, 415, 754, 829
Bse1I ACTGG 1 cut(s) 231
Bse8I GATNNNNATC 1 cut(s) 741
BseAI TCCGGA 1 cut(s) 589
BseBI CCWGG 2 cut(s) 499, 684
BseGI GGATG 3 cut(s) 665, 839, 1024
BseJI GATNNNNATC 1 cut(s) 741
BseLI CCNNNNNNNGG 4 cut(s) 15, 415, 754, 829
BseMII CTCAG 1 cut(s) 534
BseNI ACTGG 1 cut(s) 231
BseSI GKGCMC 1 cut(s) 978
BseXI GCAGC 3 cut(s) 144, 329, 977
BseYI CCCAGC 1 cut(s) 753
BsgI GTGCAG 1 cut(s) 151
Bsh1236I CGCG 2 cut(s) 313, 942
BshFI GGCC 3 cut(s) 567, 976, 1026
BsiHKAI GWGCWC 1 cut(s) 727
BsiSI CCGG 3 cut(s) 444, 488, 590
BslI CCNNNNNNNGG 4 cut(s) 15, 415, 754, 829
BsmAI GTCTC 2 cut(s) 77, 293
BsmBI CGTCTC 1 cut(s) 293
BsmI GAATGC 1 cut(s) 1183
BsnI GGCC 3 cut(s) 567, 976, 1026
Bsp120I GGGCCC 1 cut(s) 974
Bsp1286I GDGCHC 2 cut(s) 727, 978
Bsp13I TCCGGA 1 cut(s) 589
Bsp143I GATC 5 cut(s) 22, 166, 224, 742, 764
BspACI CCGC 4 cut(s) 311, 569, 940, 993
BspANI GGCC 3 cut(s) 567, 976, 1026
BspCNI CTCAG 1 cut(s) 535
BspEI TCCGGA 1 cut(s) 589
BspFNI CGCG 2 cut(s) 313, 942
BspLI GGNNCC 2 cut(s) 976, 977
BspPI GGATC 3 cut(s) 30, 174, 232
BspTI CTTAAG 1 cut(s) 425
BsrI ACTGG 1 cut(s) 231
BssMI GATC 5 cut(s) 22, 166, 224, 742, 764
BssNAI GTATAC 1 cut(s) 820
BssSI CACGAG 1 cut(s) 456
Bst1107I GTATAC 1 cut(s) 820
Bst2BI CACGAG 1 cut(s) 456
Bst2UI CCWGG 2 cut(s) 499, 684
Bst4CI ACNGT 4 cut(s) 396, 441, 733, 879
Bst6I CTCTTC 1 cut(s) 81
BstAFI CTTAAG 1 cut(s) 425
BstC8I GCNNGC 2 cut(s) 569, 1070
BstDEI CTNAG 2 cut(s) 26, 543
BstEII GGTNACC 1 cut(s) 894
BstENI CCTNNNNNAGG 1 cut(s) 13
BstF5I GGATG 3 cut(s) 665, 839, 1024
BstFNI CGCG 2 cut(s) 313, 942
BstH2I RGCGCY 1 cut(s) 754
BstHHI GCGC 2 cut(s) 609, 753
BstKTI GATC 5 cut(s) 25, 169, 227, 745, 767
BstMAI GTCTC 2 cut(s) 77, 293
BstMBI GATC 5 cut(s) 22, 166, 224, 742, 764
BstMWI GCNNNNNNNGC 2 cut(s) 722, 982
BstNI CCWGG 2 cut(s) 499, 684
BstNSI RCATGY 1 cut(s) 125
BstPI GGTNACC 1 cut(s) 894
BstSCI CCNGG 2 cut(s) 497, 682
BstSLI GKGCMC 1 cut(s) 978
BstUI CGCG 2 cut(s) 313, 942
BstV1I GCAGC 3 cut(s) 144, 329, 977
BstX2I RGATCY 2 cut(s) 22, 166
BstYI RGATCY 2 cut(s) 22, 166
BstZ17I GTATAC 1 cut(s) 820
BsuI GTATCC 1 cut(s) 842
BsuRI GGCC 3 cut(s) 567, 976, 1026
BtgZI GCGATG 1 cut(s) 957
BtrI CACGTC 1 cut(s) 463
BtsCI GGATG 3 cut(s) 665, 839, 1024
BtsIMutI CAGTG 1 cut(s) 810
Cac8I GCNNGC 2 cut(s) 569, 1070
CfoI GCGC 2 cut(s) 609, 753
Cfr13I GGNCC 4 cut(s) 566, 837, 974, 975
Csp6I GTAC 1 cut(s) 33
CviAII CATG 5 cut(s) 122, 531, 768, 1189, 1198
CviQI GTAC 1 cut(s) 33
DdeI CTNAG 2 cut(s) 26, 543
DpnI GATC 5 cut(s) 24, 168, 226, 744, 766
DpnII GATC 5 cut(s) 22, 166, 224, 742, 764
DraI TTTAAA 1 cut(s) 934
DrdI GACNNNNNNGTC 1 cut(s) 835
DseDI GACNNNNNNGTC 1 cut(s) 835
Eam1104I CTCTTC 1 cut(s) 81
EarI CTCTTC 1 cut(s) 81
Eco24I GRGCYC 1 cut(s) 978
Eco47I GGWCC 1 cut(s) 837
Eco47III AGCGCT 1 cut(s) 752
Eco57I CTGAAG 2 cut(s) 834, 1038
Eco91I GGTNACC 1 cut(s) 894
EcoNI CCTNNNNNAGG 1 cut(s) 13
EcoO109I RGGNCCY 2 cut(s) 974, 975
EcoO65I GGTNACC 1 cut(s) 894
EcoRII CCWGG 2 cut(s) 497, 682
EcoT22I ATGCAT 1 cut(s) 809
EcoT38I GRGCYC 1 cut(s) 978
Esp3I CGTCTC 1 cut(s) 293
FaeI CATG 5 cut(s) 125, 534, 771, 1192, 1201
FatI CATG 5 cut(s) 121, 530, 767, 1188, 1197
FauI CCCGC 2 cut(s) 576, 947
FauNDI CATATG 1 cut(s) 1088
FblI GTMKAC 1 cut(s) 819
Fnu4HI GCNGC 3 cut(s) 133, 343, 991
FokI GGATG 3 cut(s) 672, 846, 1031
FriOI GRGCYC 1 cut(s) 978
Fsp4HI GCNGC 3 cut(s) 133, 343, 991
FspBI CTAG 2 cut(s) 155, 329
GlaI GCGC 2 cut(s) 608, 752
GluI GCNGC 3 cut(s) 133, 343, 991
GsaI CCCAGC 1 cut(s) 757
HaeII RGCGCY 1 cut(s) 754
HaeIII GGCC 3 cut(s) 567, 976, 1026
HapII CCGG 3 cut(s) 444, 488, 590
HhaI GCGC 2 cut(s) 609, 753
Hin1II CATG 5 cut(s) 125, 534, 771, 1192, 1201
Hin6I GCGC 2 cut(s) 607, 751
HinP1I GCGC 2 cut(s) 607, 751
HincII GTYRAC 3 cut(s) 93, 504, 796
HindII GTYRAC 3 cut(s) 93, 504, 796
HinfI GANTC 2 cut(s) 586, 996
HpaI GTTAAC 2 cut(s) 93, 796
HpaII CCGG 3 cut(s) 444, 488, 590
HphI GGTGA 3 cut(s) 484, 737, 888
Hpy166II GTNNAC 9 cut(s) 93, 236, 392, 504, 600, 796, 820, 1000, 1111
Hpy188I TCNGA 3 cut(s) 44, 544, 776
Hpy188III TCNNGA 4 cut(s) 83, 155, 458, 590
Hpy8I GTNNAC 9 cut(s) 93, 236, 392, 504, 600, 796, 820, 1000, 1111
HpyAV CCTTC 9 cut(s) 63, 380, 620, 635, 650, 915, 965, 1016, 1062
HpyCH4III ACNGT 4 cut(s) 396, 441, 733, 879
HpyCH4IV ACGT 3 cut(s) 462, 792, 1002
HpyCH4V TGCA 8 cut(s) 38, 132, 148, 282, 302, 355, 807, 985
HpyF10VI GCNNNNNNNGC 2 cut(s) 722, 982
HpyF3I CTNAG 2 cut(s) 26, 543
HpySE526I ACGT 3 cut(s) 462, 792, 1002
Hsp92II CATG 5 cut(s) 125, 534, 771, 1192, 1201
HspAI GCGC 2 cut(s) 607, 751
Kpn2I TCCGGA 1 cut(s) 589
KspAI GTTAAC 2 cut(s) 93, 796
Kzo9I GATC 5 cut(s) 22, 166, 224, 742, 764
Lsp1109I GCAGC 3 cut(s) 144, 329, 977
LweI GCATC 3 cut(s) 322, 816, 972
MaeI CTAG 2 cut(s) 155, 329
MaeII ACGT 3 cut(s) 462, 792, 1002
MaeIII GTNAC 4 cut(s) 190, 458, 781, 894
MalI GATC 5 cut(s) 24, 168, 226, 744, 766
MboI GATC 5 cut(s) 22, 166, 224, 742, 764
MboII GAAGA 2 cut(s) 98, 1145
MfeI CAATTG 1 cut(s) 277
MflI RGATCY 2 cut(s) 22, 166
MhlI GDGCHC 2 cut(s) 727, 978
MlyI GAGTC 1 cut(s) 1005
MmeI TCCRAC 1 cut(s) 224
MnlI CCTC 3 cut(s) 694, 1009, 1088
Mph1103I ATGCAT 1 cut(s) 809
MroI TCCGGA 1 cut(s) 589
MroXI GAANNNNTTC 2 cut(s) 630, 645
MseI TTAA 9 cut(s) 78, 92, 117, 426, 561, 795, 921, 933, 963
MslI CAYNNNNRTG 1 cut(s) 1128
MspCI CTTAAG 1 cut(s) 425
MspI CCGG 3 cut(s) 444, 488, 590
MspR9I CCNGG 2 cut(s) 499, 684
MunI CAATTG 1 cut(s) 277
Mva1269I GAATGC 1 cut(s) 1183
MvaI CCWGG 2 cut(s) 499, 684
MvnI CGCG 2 cut(s) 313, 942
MwoI GCNNNNNNNGC 2 cut(s) 722, 982
NdeI CATATG 1 cut(s) 1088
NdeII GATC 5 cut(s) 22, 166, 224, 742, 764
NlaIII CATG 5 cut(s) 125, 534, 771, 1192, 1201
NlaIV GGNNCC 2 cut(s) 976, 977
NmuCI GTSAC 3 cut(s) 458, 781, 894
NsiI ATGCAT 1 cut(s) 809
NspI RCATGY 1 cut(s) 125
PciI ACATGT 1 cut(s) 121
PctI GAATGC 1 cut(s) 1183
PdmI GAANNNNTTC 2 cut(s) 630, 645
PfeI GAWTC 1 cut(s) 586
PfoI TCCNGGA 1 cut(s) 682
PkrI GCNGC 3 cut(s) 134, 344, 992
PleI GAGTC 1 cut(s) 1004
PpsI GAGTC 1 cut(s) 1004
PscI ACATGT 1 cut(s) 121
PshBI ATTAAT 1 cut(s) 78
Psp6I CCWGG 2 cut(s) 497, 682
PspEI GGTNACC 1 cut(s) 894
PspFI CCCAGC 1 cut(s) 753
PspGI CCWGG 2 cut(s) 497, 682
PspN4I GGNNCC 2 cut(s) 976, 977
PspOMI GGGCCC 1 cut(s) 974
PspPI GGNCC 4 cut(s) 566, 837, 974, 975
PsuI RGATCY 2 cut(s) 22, 166
RsaI GTAC 1 cut(s) 34
RsaNI GTAC 1 cut(s) 33
RseI CAYNNNNRTG 1 cut(s) 1128
SaqAI TTAA 9 cut(s) 78, 92, 117, 426, 561, 795, 921, 933, 963
SatI GCNGC 3 cut(s) 133, 343, 991
Sau3AI GATC 5 cut(s) 22, 166, 224, 742, 764
Sau96I GGNCC 4 cut(s) 566, 837, 974, 975
SchI GAGTC 1 cut(s) 1005
ScrFI CCNGG 2 cut(s) 499, 684
SduI GDGCHC 2 cut(s) 727, 978
SfaNI GCATC 3 cut(s) 322, 816, 972
SinI GGWCC 1 cut(s) 837
SmiMI CAYNNNNRTG 1 cut(s) 1128
SmlI CTYRAG 1 cut(s) 425
SmoI CTYRAG 1 cut(s) 425
SsiI CCGC 4 cut(s) 311, 569, 940, 993
SspMI CTAG 2 cut(s) 155, 329
StyD4I CCNGG 2 cut(s) 497, 682
TaaI ACNGT 4 cut(s) 396, 441, 733, 879
TaiI ACGT 3 cut(s) 465, 795, 1005
TaqI TCGA 3 cut(s) 227, 618, 861
TfiI GAWTC 1 cut(s) 586
Tru1I TTAA 9 cut(s) 78, 92, 117, 426, 561, 795, 921, 933, 963
Tru9I TTAA 9 cut(s) 78, 92, 117, 426, 561, 795, 921, 933, 963
TscAI CASTG 1 cut(s) 817
TseFI GTSAC 3 cut(s) 458, 781, 894
TseI GCWGC 3 cut(s) 132, 342, 990
Tsp45I GTSAC 3 cut(s) 458, 781, 894
TspDTI ATGAA 5 cut(s) 17, 45, 450, 1035, 1146
TspGWI ACGGA 2 cut(s) 829, 898
TspRI CASTG 1 cut(s) 817
Vha464I CTTAAG 1 cut(s) 425
VpaK11BI GGWCC 1 cut(s) 837
VspI ATTAAT 1 cut(s) 78
XagI CCTNNNNNAGG 1 cut(s) 13
XapI RAATTY 8 cut(s) 306, 360, 481, 578, 665, 688, 929, 1115
XbaI TCTAGA 1 cut(s) 154
XceI RCATGY 1 cut(s) 125
XmiI GTMKAC 1 cut(s) 819
XmnI GAANNNNTTC 2 cut(s) 630, 645
XspI CTAG 2 cut(s) 155, 329
Zsp2I ATGCAT 1 cut(s) 809
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.