Rmu_sc0007326.1_g000001

Nuclear fragile X mental retardation-interacting protein 1 (NUFIP1)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0007326.1
OG0005893
Physical Location & Seq
Forward (+)
193 .. 6159
5967 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1539 bp
atgggacctcagcccggtttcatgaatttatctaacaatcttcttccgatgcaaaataaccatatgcgtgtgcccatgtcgcaatttggtacccttggtccgatatctcaaccaggttatcccagtgttggattttgtggtccacagaataatgcactcaatatgaacttggctgcttcctatccatttaacgggcagttatgcaatatggggcagaatctcaatcaagtaaatcaaaatatgggttttctacctggtcaattgtttggacagaacctgttgaatttacagctgatgaatcaaaatattgggccatatgggcaattctgttctccgaatctgttgaataggaatcaggttgtgccagtgcaaatgcaaatgcaaaacccttgccaaggtggtcctaattatgcatttggggggtcaaatcaagcacctcaggctgcagttctgcaaaatcctgctttttttgcaactcctaattctggtaatgtgcactctccaaatcaggccggacaacaaatcaaccagaatcagcaaaacaacgtcttccgatggacacagggaaatcatataaaagctggtggcgtttacaattcgaattctgacagaaaaacttcgccaagcaaaaattttataaagcatccaaaacgagggttgcaaccgcaagggggatttcagaattctcagtttcatcacatcaaaaatgcaaagggaaagtttgcttttcccaacaggcaaaacagaaaagggctaagcaatgagaaggaaggaaaatctgctcttcccgactctatgaatcaaggcagggaaaggaaaagatctctttctttgtcctatacggaacaagaagttcaactgtggcgtgaaggacgtaggaagaactacccatcaaacaccaacatagagaagaagctaagtgaaaagatgatagactcagaggccattgagagagaggccaaagcacgaagggagcaactcaaggagattcttacaaagcaggctgagatgggagttgaagtcgctgagataccatctcactacctctcagattcagcaaaacaagggaatggaagagaggagaataagtcatggactgacaatcggagatcacaaaacaagttcggtaagagagacagatatgataaggaccggtttgcaaagaggcaaagatcaggtcagaaggattcatctaatgacccttcattcagcaaaagggagccaaccttactgcagaaacttcttagttcagatatcaagagagataaaagtcacttatgccaggtgtttaggttcatggtgacaaactctttctttaaagattttcctgacaaacctttgaaatttcctactgtagtagttaaagaaagcgtggtggaggaaggtgtggttgaagagtcttcagttgccgggaaaggtgcttatgggggccgtgacaagtccatgtttgaaaaagttggtaacaatgattataaagattgttgtcacaatgacgattgtgttgaagatgaggaagaagagggagagatcaacatctag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03015 ko05164 map03015 map05164
Pfam Domains
Protein Families
NUFIP1

Protein Analysis

512

Amino Acids

57.64

Weight (kDa)

9.32

Isoelectric Point (pI)

50.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NUFIP1 PF10453 277 - 309 2.3e-08 FMR1-interacting protein 1 (NUFIP1)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 638, 1473
Acc65I GGTACC 1 cut(s) 89
AccB1I GGYRCC 1 cut(s) 89
AciI CCGC 1 cut(s) 665
AcsI RAATTY 6 cut(s) 25, 283, 601, 631, 682, 1343
AcuI CTGAAG 1 cut(s) 1386
AfaI GTAC 1 cut(s) 91
AfiI CCNNNNNNNGG 7 cut(s) 14, 128, 191, 395, 485, 653, 671
AgeI ACCGGT 1 cut(s) 1152
AgsI TTSAA 8 cut(s) 283, 346, 857, 1019, 1342, 1394, 1451, 1505
AjnI CCWGG 3 cut(s) 112, 253, 1281
AluBI AGCT 3 cut(s) 292, 581, 916
AluI AGCT 3 cut(s) 292, 581, 916
Alw21I GWGCWC 1 cut(s) 498
Alw26I GTCTC 1 cut(s) 1128
Alw44I GTGCAC 1 cut(s) 494
AlwNI CAGNNNCTG 1 cut(s) 277
AoxI GGCC 5 cut(s) 311, 510, 942, 957, 1429
ApaLI GTGCAC 1 cut(s) 494
ApeKI GCWGC 2 cut(s) 173, 443
ApoI RAATTY 6 cut(s) 25, 283, 601, 631, 682, 1343
ArsI GACNNNNNNTTYG 2 cut(s) 1162, 1194
AsiGI ACCGGT 1 cut(s) 1152
Asp700I GAANNNNTTC 1 cut(s) 616
Asp718I GGTACC 1 cut(s) 89
AspS9I GGNCC 7 cut(s) 5, 98, 140, 311, 401, 1150, 1429
AsuC2I CCSGG 2 cut(s) 15, 1411
AsuHPI GGTGA 1 cut(s) 1312
AsuII TTCGAA 1 cut(s) 599
AvaII GGWCC 5 cut(s) 5, 98, 140, 401, 1150
AxyI CCTNAGG 1 cut(s) 438
BaeGI GKGCMC 2 cut(s) 75, 498
BanI GGYRCC 1 cut(s) 89
BbsI GAAGAC 2 cut(s) 541, 1392
Bbv12I GWGCWC 1 cut(s) 498
BbvCI CCTCAGC 1 cut(s) 9
BbvI GCAGC 2 cut(s) 160, 430
BccI CCATC 4 cut(s) 549, 898, 1003, 1042
BceAI ACGGC 1 cut(s) 1416
BciT130I CCWGG 3 cut(s) 114, 255, 1283
BcnI CCSGG 2 cut(s) 15, 1411
BcoDI GTCTC 1 cut(s) 1128
BfaI CTAG 1 cut(s) 1537
BfmI CTRYAG 3 cut(s) 444, 1232, 1353
BglI GCCNNNNNGGC 1 cut(s) 319
BglII AGATCT 1 cut(s) 821
BisI GCNGC 2 cut(s) 174, 444
BlpI GCTNAGC 1 cut(s) 755
BlsI GCNGC 2 cut(s) 175, 445
Bme1390I CCNGG 5 cut(s) 15, 114, 255, 1283, 1411
Bme18I GGWCC 5 cut(s) 5, 98, 140, 401, 1150
BmgT120I GGNCC 7 cut(s) 5, 98, 140, 311, 401, 1150, 1429
BmiI GGNNCC 4 cut(s) 6, 91, 1221, 1430
BmrFI CCNGG 5 cut(s) 15, 114, 255, 1283, 1411
BmrI ACTGGG 1 cut(s) 117
BmsI GCATC 2 cut(s) 39, 652
BmuI ACTGGG 1 cut(s) 117
BpiI GAAGAC 2 cut(s) 541, 1392
Bpu10I CCTNAGC 1 cut(s) 9
Bpu1102I GCTNAGC 1 cut(s) 755
Bpu14I TTCGAA 1 cut(s) 599
BpuEI CTTGAG 1 cut(s) 965
BpuMI CCSGG 2 cut(s) 15, 1411
BsaBI GATNNNNATC 1 cut(s) 1532
BsaJI CCNNGG 2 cut(s) 94, 394
BsaWI WCCGGW 1 cut(s) 1152
BsaXI ACNNNNNCTCC 4 cut(s) 1005, 1035, 1073, 1103
Bsc4I CCNNNNNNNGG 7 cut(s) 14, 128, 191, 395, 485, 653, 671
Bse118I RCCGGY 1 cut(s) 1152
Bse1I ACTGG 2 cut(s) 123, 365
Bse21I CCTNAGG 1 cut(s) 438
Bse3DI GCAATG 1 cut(s) 766
Bse8I GATNNNNATC 1 cut(s) 1532
BseBI CCWGG 3 cut(s) 114, 255, 1283
BseDI CCNNGG 2 cut(s) 94, 394
BseGI GGATG 1 cut(s) 643
BseJI GATNNNNATC 1 cut(s) 1532
BseLI CCNNNNNNNGG 7 cut(s) 14, 128, 191, 395, 485, 653, 671
BseMI GCAATG 1 cut(s) 766
BseMII CTCAG 7 cut(s) 23, 452, 701, 951, 996, 1017, 1062
BseNI ACTGG 2 cut(s) 123, 365
BseRI GAGGAG 1 cut(s) 1094
BseSI GKGCMC 2 cut(s) 75, 498
BseXI GCAGC 2 cut(s) 160, 430
BshFI GGCC 5 cut(s) 313, 512, 944, 959, 1431
BshNI GGYRCC 1 cut(s) 89
BshTI ACCGGT 1 cut(s) 1152
BsiHKAI GWGCWC 1 cut(s) 498
BsiSI CCGG 4 cut(s) 15, 513, 1153, 1410
BslFI GGGAC 1 cut(s) 18
BslI CCNNNNNNNGG 7 cut(s) 14, 128, 191, 395, 485, 653, 671
BsmAI GTCTC 1 cut(s) 1128
BsmFI GGGAC 1 cut(s) 18
BsnI GGCC 5 cut(s) 313, 512, 944, 959, 1431
Bsp119I TTCGAA 1 cut(s) 599
Bsp1286I GDGCHC 2 cut(s) 75, 498
Bsp143I GATC 4 cut(s) 821, 1109, 1172, 1527
Bsp1720I GCTNAGC 1 cut(s) 755
BspACI CCGC 1 cut(s) 665
BspANI GGCC 5 cut(s) 313, 512, 944, 959, 1431
BspCNI CTCAG 7 cut(s) 22, 451, 700, 950, 997, 1018, 1061
BspHI TCATGA 1 cut(s) 21
BspLI GGNNCC 4 cut(s) 6, 91, 1221, 1430
BspMAI CTGCAG 2 cut(s) 448, 1236
BspQI GCTCTTC 1 cut(s) 789
BspT104I TTCGAA 1 cut(s) 599
BspT107I GGYRCC 1 cut(s) 89
BsrDI GCAATG 1 cut(s) 766
BsrFI RCCGGY 1 cut(s) 1152
BsrI ACTGG 2 cut(s) 123, 365
BssAI RCCGGY 1 cut(s) 1152
BssECI CCNNGG 2 cut(s) 94, 394
BssMI GATC 4 cut(s) 821, 1109, 1172, 1527
BssT1I CCWWGG 2 cut(s) 94, 394
Bst2UI CCWGG 3 cut(s) 114, 255, 1283
Bst4CI ACNGT 2 cut(s) 861, 1354
Bst6I CTCTTC 4 cut(s) 789, 1069, 1389, 1512
BstBI TTCGAA 1 cut(s) 599
BstC8I GCNNGC 1 cut(s) 1002
BstENI CCTNNNNNAGG 1 cut(s) 393
BstF5I GGATG 1 cut(s) 643
BstKTI GATC 4 cut(s) 824, 1112, 1175, 1530
BstMAI GTCTC 1 cut(s) 1128
BstMBI GATC 4 cut(s) 821, 1109, 1172, 1527
BstMWI GCNNNNNNNGC 4 cut(s) 79, 319, 440, 470
BstNI CCWGG 3 cut(s) 114, 255, 1283
BstSCI CCNGG 5 cut(s) 13, 112, 253, 1281, 1409
BstSFI CTRYAG 3 cut(s) 444, 1232, 1353
BstSLI GKGCMC 2 cut(s) 75, 498
BstV1I GCAGC 2 cut(s) 160, 430
BstV2I GAAGAC 2 cut(s) 541, 1392
BstX2I RGATCY 1 cut(s) 821
BstYI RGATCY 1 cut(s) 821
Bsu36I CCTNAGG 1 cut(s) 438
BsuRI GGCC 5 cut(s) 313, 512, 944, 959, 1431
BtsCI GGATG 1 cut(s) 643
BtsIMutI CAGTG 2 cut(s) 130, 372
Cac8I GCNNGC 1 cut(s) 1002
CaiI CAGNNNCTG 1 cut(s) 277
CciI TCATGA 1 cut(s) 21
Cfr10I RCCGGY 1 cut(s) 1152
Cfr13I GGNCC 7 cut(s) 5, 98, 140, 311, 401, 1150, 1429
CsiI ACCWGGT 2 cut(s) 112, 253
Csp6I GTAC 1 cut(s) 90
CspAI ACCGGT 1 cut(s) 1152
CviAII CATG 5 cut(s) 22, 76, 1092, 1297, 1444
CviQI GTAC 1 cut(s) 90
DpnI GATC 4 cut(s) 823, 1111, 1174, 1529
DpnII GATC 4 cut(s) 821, 1109, 1172, 1527
DraI TTTAAA 1 cut(s) 1318
Eam1104I CTCTTC 4 cut(s) 789, 1069, 1389, 1512
EarI CTCTTC 4 cut(s) 789, 1069, 1389, 1512
Eco130I CCWWGG 2 cut(s) 94, 394
Eco32I GATATC 2 cut(s) 105, 1255
Eco47I GGWCC 5 cut(s) 5, 98, 140, 401, 1150
Eco57I CTGAAG 1 cut(s) 1386
Eco81I CCTNAGG 1 cut(s) 438
EcoNI CCTNNNNNAGG 1 cut(s) 393
EcoO109I RGGNCCY 1 cut(s) 5
EcoRI GAATTC 2 cut(s) 601, 682
EcoRII CCWGG 3 cut(s) 112, 253, 1281
EcoRV GATATC 2 cut(s) 105, 1255
EcoT14I CCWWGG 2 cut(s) 94, 394
EcoT22I ATGCAT 1 cut(s) 415
ErhI CCWWGG 2 cut(s) 94, 394
FaeI CATG 5 cut(s) 25, 79, 1095, 1300, 1447
FaqI GGGAC 1 cut(s) 18
FatI CATG 5 cut(s) 21, 75, 1091, 1296, 1443
FauNDI CATATG 2 cut(s) 63, 316
Fnu4HI GCNGC 2 cut(s) 174, 444
FokI GGATG 1 cut(s) 630
Fsp4HI GCNGC 2 cut(s) 174, 444
FspBI CTAG 1 cut(s) 1537
GluI GCNGC 2 cut(s) 174, 444
HaeIII GGCC 5 cut(s) 313, 512, 944, 959, 1431
HapII CCGG 4 cut(s) 15, 513, 1153, 1410
Hin1II CATG 5 cut(s) 25, 79, 1095, 1300, 1447
HpaII CCGG 4 cut(s) 15, 513, 1153, 1410
HphI GGTGA 1 cut(s) 1312
Hpy166II GTNNAC 3 cut(s) 143, 496, 592
Hpy188III TCNNGA 4 cut(s) 22, 788, 1258, 1328
Hpy8I GTNNAC 3 cut(s) 143, 496, 592
HpyAV CCTTC 7 cut(s) 760, 764, 863, 963, 1177, 1212, 1376
HpyCH4III ACNGT 2 cut(s) 861, 1354
HpyCH4IV ACGT 2 cut(s) 546, 874
HpyF10VI GCNNNNNNNGC 4 cut(s) 79, 319, 440, 470
HpySE526I ACGT 2 cut(s) 546, 874
Hsp92II CATG 5 cut(s) 25, 79, 1095, 1300, 1447
KpnI GGTACC 1 cut(s) 93
Kzo9I GATC 4 cut(s) 821, 1109, 1172, 1527
LguI GCTCTTC 1 cut(s) 789
LmnI GCTCC 2 cut(s) 973, 1219
Lsp1109I GCAGC 2 cut(s) 160, 430
LweI GCATC 2 cut(s) 39, 652
MabI ACCWGGT 2 cut(s) 112, 253
MaeI CTAG 1 cut(s) 1537
MaeII ACGT 2 cut(s) 546, 874
MaeIII GTNAC 5 cut(s) 1271, 1300, 1433, 1460, 1484
MalI GATC 4 cut(s) 823, 1111, 1174, 1529
MboI GATC 4 cut(s) 821, 1109, 1172, 1527
MfeI CAATTG 1 cut(s) 260
MflI RGATCY 1 cut(s) 821
MhlI GDGCHC 2 cut(s) 75, 498
MlyI GAGTC 3 cut(s) 785, 929, 1406
MmeI TCCRAC 1 cut(s) 109
Mph1103I ATGCAT 1 cut(s) 415
MroXI GAANNNNTTC 1 cut(s) 616
MseI TTAA 3 cut(s) 189, 1317, 1362
MslI CAYNNNNRTG 1 cut(s) 66
MspA1I CMGCKG 1 cut(s) 292
MspI CCGG 4 cut(s) 15, 513, 1153, 1410
MspR9I CCNGG 5 cut(s) 15, 114, 255, 1283, 1411
MunI CAATTG 1 cut(s) 260
MvaI CCWGG 3 cut(s) 114, 255, 1283
MwoI GCNNNNNNNGC 4 cut(s) 79, 319, 440, 470
NciI CCSGG 2 cut(s) 15, 1411
NdeI CATATG 2 cut(s) 63, 316
NdeII GATC 4 cut(s) 821, 1109, 1172, 1527
NlaIII CATG 5 cut(s) 25, 79, 1095, 1300, 1447
NlaIV GGNNCC 4 cut(s) 6, 91, 1221, 1430
NmuCI GTSAC 4 cut(s) 1271, 1300, 1433, 1484
NsiI ATGCAT 1 cut(s) 415
NspV TTCGAA 1 cut(s) 599
PagI TCATGA 1 cut(s) 21
PciSI GCTCTTC 1 cut(s) 789
PdmI GAANNNNTTC 1 cut(s) 616
PfeI GAWTC 9 cut(s) 217, 298, 337, 352, 532, 799, 988, 1052, 1187
PinAI ACCGGT 1 cut(s) 1152
PkrI GCNGC 2 cut(s) 175, 445
PleI GAGTC 3 cut(s) 785, 929, 1405
PpsI GAGTC 3 cut(s) 785, 929, 1405
PpuMI RGGWCCY 1 cut(s) 5
PsiI TTATAA 2 cut(s) 638, 1473
Psp5II RGGWCCY 1 cut(s) 5
Psp6I CCWGG 3 cut(s) 112, 253, 1281
PspGI CCWGG 3 cut(s) 112, 253, 1281
PspN4I GGNNCC 4 cut(s) 6, 91, 1221, 1430
PspPI GGNCC 7 cut(s) 5, 98, 140, 311, 401, 1150, 1429
PspPPI RGGWCCY 1 cut(s) 5
PstI CTGCAG 2 cut(s) 448, 1236
PstNI CAGNNNCTG 1 cut(s) 277
PsuI RGATCY 1 cut(s) 821
PvuII CAGCTG 1 cut(s) 292
RsaI GTAC 1 cut(s) 91
RsaNI GTAC 1 cut(s) 90
RseI CAYNNNNRTG 1 cut(s) 66
SapI GCTCTTC 1 cut(s) 789
SaqAI TTAA 3 cut(s) 189, 1317, 1362
SatI GCNGC 2 cut(s) 174, 444
Sau3AI GATC 4 cut(s) 821, 1109, 1172, 1527
Sau96I GGNCC 7 cut(s) 5, 98, 140, 311, 401, 1150, 1429
SchI GAGTC 3 cut(s) 785, 929, 1406
ScrFI CCNGG 5 cut(s) 15, 114, 255, 1283, 1411
SduI GDGCHC 2 cut(s) 75, 498
SexAI ACCWGGT 2 cut(s) 112, 253
SfaNI GCATC 2 cut(s) 39, 652
SfcI CTRYAG 3 cut(s) 444, 1232, 1353
SfuI TTCGAA 1 cut(s) 599
SinI GGWCC 5 cut(s) 5, 98, 140, 401, 1150
SmiMI CAYNNNNRTG 1 cut(s) 66
SmlI CTYRAG 1 cut(s) 980
SmoI CTYRAG 1 cut(s) 980
SsiI CCGC 1 cut(s) 665
SspI AATATT 1 cut(s) 307
SspMI CTAG 1 cut(s) 1537
StyD4I CCNGG 5 cut(s) 13, 112, 253, 1281, 1409
StyI CCWWGG 2 cut(s) 94, 394
TaaI ACNGT 2 cut(s) 861, 1354
TaiI ACGT 2 cut(s) 549, 877
TaqI TCGA 1 cut(s) 599
TfiI GAWTC 9 cut(s) 217, 298, 337, 352, 532, 799, 988, 1052, 1187
Tru1I TTAA 3 cut(s) 189, 1317, 1362
Tru9I TTAA 3 cut(s) 189, 1317, 1362
TscAI CASTG 2 cut(s) 130, 372
TseFI GTSAC 4 cut(s) 1271, 1300, 1433, 1484
TseI GCWGC 2 cut(s) 173, 443
Tsp45I GTSAC 4 cut(s) 1271, 1300, 1433, 1484
TspDTI ATGAA 9 cut(s) 10, 38, 179, 311, 683, 812, 1179, 1194, 1285
TspGWI ACGGA 1 cut(s) 857
TspRI CASTG 2 cut(s) 130, 372
VneI GTGCAC 1 cut(s) 494
VpaK11BI GGWCC 5 cut(s) 5, 98, 140, 401, 1150
XagI CCTNNNNNAGG 1 cut(s) 393
XapI RAATTY 6 cut(s) 25, 283, 601, 631, 682, 1343
XmnI GAANNNNTTC 1 cut(s) 616
XspI CTAG 1 cut(s) 1537
Zsp2I ATGCAT 1 cut(s) 415
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.