Rmu_sc0007360.1_g000007
NAC Family

Probably inactive leucine-rich repeat receptor-like protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0007360.1
OG0002722
Physical Location & Seq
Reverse (-)
33484 .. 36275
2792 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 2019 bp
atggctaggcttatgcgcgcaccaaacccatcattcctgctcgctctcattgtagctacgctttcttttatgaatatgttgtcttttggtttgccggagaccacgtccgacgccctgttaaaattcaaaagttctttggggaacagttccgaagctcttaggaattggggtagttctccgaacccatgcaatggtaacagggggaattgggtcggtgtgctttgctttcaagggaatgttaggggcttgaaactcgaaggcatggggttacagggaacgttagatatggaacctctcgcgtcgttgccatatttgaagactttaagcttcacgaacaattcattcataggtccgttgcctaatctcactagactaaagagattagggtcgttatatttgtcagataatcatttgtctggggatattcctgataatgcatttgagaatttgactttcttgaagaaactgtatttgggaaataatgagttctcaggcaaaatcccttcatctcttgccaaattgccaaaccttttcgacgtggggcttgaaaataactattttagtggcgaaatccctgattttaaaagtggggactcagagaaattatggaggttgtatttggctaacaatgagttggagggagaaatcccaaaaagcctgagaacgcaggagcctagcagttttgcaggaaatgaaaacctatgtggtccgcctatagaagcatgtagcccctctcctgctcctgctcctgcatcgtctccatcgccatcttcgcctccatgtgggggatccggggaaccatgcagcccttccgaccctcctacgaagtcttcatctcttggtctaaaaatagccttgggtatagtgagtattctgctcctattagttatcattgctgtacttttgatgttcctcagaaagagaaagcaacaaccggaattagaccaggcggagttgttggatgaatcctccaagtacacagcagcaaccgcgagccagatggatgccaagtctgtagaaaataataatgtgaacaatccaccaaggaggggtgagcaagcaggaaagctgtcgtttgtgagggatgacagggacaggtttgatttgcatgacctgctaagagcctcagccgaaatattggggagtgggaactttggggcttcatacaaggctttgatactaactgatgctgtagtggtcaagaggtataagcagatgaacaatgtgggaagagaggagttccatgaccacatgagaaggcttgggagattgacacaccaaaacttgttgccaatcgtggcctattattatagaagggaagagaagctcttggtttctgactttgtggagaacggtagcttggcttctcatcttcatggcaatcacaatctgcataagcctggacttgactggcctacccgtttgagaataatcaaaggaataggaaggggcctgacatacctctacaacacactcccaagcttggttgtacctcatggtcatctgaaatcctccaatgtgcttctggacgaaaacatggagcctctactgaacgactatgctctactccccgtgatcaacatggaacaagcccaacacctcatgatggcttacaagtcacccgaatacgcgcggcaccaccggataacaaagaagaccgacgtgtggtgcttcgggataatcatgttggaggtgttgacaggcaagttcccagaaaactatctaaagcaaagttatgacagtaaggcggatttggcgagctgggtgaacggaatgatcaaggagaagaagactagtgaggtgtttgatggagaaatggggaatgtcggtgacagcaaaggtgaacttctaaagctgttgaagattggagtgagttgttgtgaggtggatgtggagagaaggctggacttgcatgaagttgttgcaaagattgaggagttgaatgagggagaaagtgatggggaataccgctcaagtgtttcaagtgaagaagatgattatacttctcaggctgtgtga

Protein Analysis

672

Amino Acids

74.3

Weight (kDa)

5.99

Isoelectric Point (pI)

39.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 37 - 75 7.2e-09 Leucine rich repeat N-terminal domain
LRR_8 PF13855 127 - 186 5.4e-08 Leucine rich repeat
PK_Tyr_Ser-Thr PF07714 378 - 643 2.6e-27 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 378 - 642 8.5e-24 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1122
AccB1I GGYRCC 1 cut(s) 1635
AccB7I CCANNNNNTGG 1 cut(s) 191
AccBSI CCGCTC 1 cut(s) 1971
AccII CGCG 5 cut(s) 18, 299, 992, 1631, 1633
AciI CCGC 6 cut(s) 710, 950, 990, 1633, 1748, 1969
AclI AACGTT 1 cut(s) 278
AclWI GGATC 2 cut(s) 783, 796
AcsI RAATTY 2 cut(s) 122, 445
AcyI GRCGYC 1 cut(s) 111
AfaI GTAC 3 cut(s) 900, 977, 1491
AfiI CCNNNNNNNGG 7 cut(s) 191, 782, 785, 1049, 1483, 1606, 1665
AflIII ACRYGT 1 cut(s) 1662
AgsI TTSAA 9 cut(s) 127, 230, 250, 316, 460, 548, 1861, 1942, 1983
AhlI ACTAGT 1 cut(s) 1793
AjiI CACGTC 3 cut(s) 105, 538, 1663
AjnI CCWGG 2 cut(s) 945, 1399
AjuI GAANNNNNNNTTGG 4 cut(s) 455, 487, 1343, 1375
AluBI AGCT 9 cut(s) 56, 155, 327, 1069, 1327, 1359, 1482, 1761, 1855
AluI AGCT 9 cut(s) 56, 155, 327, 1069, 1327, 1359, 1482, 1761, 1855
Alw26I GTCTC 2 cut(s) 92, 762
AlwI GGATC 2 cut(s) 783, 796
AoxI GGCC 3 cut(s) 1299, 1412, 1450
ApeKI GCWGC 2 cut(s) 804, 983
ApoI RAATTY 2 cut(s) 122, 445
AspLEI GCGC 3 cut(s) 18, 20, 1633
AspS9I GGNCC 3 cut(s) 350, 707, 1450
AsuC2I CCSGG 1 cut(s) 793
AsuHPI GGTGA 5 cut(s) 1064, 1611, 1777, 1841, 1853
AvaII GGWCC 2 cut(s) 350, 707
BamHI GGATCC 1 cut(s) 788
BanI GGYRCC 1 cut(s) 1635
BarI GAAGNNNNNNTAC 2 cut(s) 1348, 1380
BbsI GAAGAC 4 cut(s) 323, 822, 1661, 1796
BbvCI CCTCAGC 1 cut(s) 1126
BbvI GCAGC 2 cut(s) 816, 995
BccI CCATC 7 cut(s) 37, 769, 775, 994, 1600, 1802, 1952
BciT130I CCWGG 2 cut(s) 947, 1401
BclI TGATCA 2 cut(s) 1575, 1776
BcnI CCSGG 1 cut(s) 793
BcoDI GTCTC 2 cut(s) 92, 762
BcuI ACTAGT 1 cut(s) 1793
BfaI CTAG 4 cut(s) 6, 369, 675, 1794
BfmI CTRYAG 3 cut(s) 714, 1014, 1191
BfuAI ACCTGC 1 cut(s) 1122
BisI GCNGC 3 cut(s) 805, 984, 1634
BlsI GCNGC 3 cut(s) 806, 985, 1635
Bme1390I CCNGG 3 cut(s) 793, 947, 1401
Bme18I GGWCC 2 cut(s) 350, 707
BmgBI CACGTC 3 cut(s) 105, 538, 1663
BmgT120I GGNCC 3 cut(s) 350, 707, 1450
BmiI GGNNCC 7 cut(s) 291, 672, 790, 798, 1451, 1542, 1637
BmrFI CCNGG 3 cut(s) 793, 947, 1401
BmsI GCATC 3 cut(s) 761, 994, 1177
BpiI GAAGAC 4 cut(s) 323, 822, 1661, 1796
Bpu10I CCTNAGC 1 cut(s) 1126
BpuEI CTTGAG 1 cut(s) 1957
BpuMI CCSGG 1 cut(s) 793
BsaHI GRCGYC 1 cut(s) 111
BsaI GGTCTC 1 cut(s) 92
BsaJI CCNNGG 3 cut(s) 792, 855, 1043
BsaWI WCCGGW 2 cut(s) 934, 1641
BsaXI ACNNNNNCTCC 2 cut(s) 89, 119
Bsc4I CCNNNNNNNGG 7 cut(s) 191, 782, 785, 1049, 1483, 1606, 1665
Bse1I ACTGG 1 cut(s) 1415
Bse3DI GCAATG 2 cut(s) 196, 891
BseBI CCWGG 2 cut(s) 947, 1401
BseDI CCNNGG 3 cut(s) 792, 855, 1043
BseGI GGATG 4 cut(s) 967, 1009, 1090, 1894
BseLI CCNNNNNNNGG 7 cut(s) 191, 782, 785, 1049, 1483, 1606, 1665
BseMI GCAATG 2 cut(s) 196, 891
BseMII CTCAG 5 cut(s) 504, 609, 650, 928, 1140
BseNI ACTGG 1 cut(s) 1415
BsePI GCGCGC 1 cut(s) 16
BseRI GAGGAG 2 cut(s) 1250, 1949
BseXI GCAGC 2 cut(s) 816, 995
BseYI CCCAGC 1 cut(s) 1761
Bsh1236I CGCG 5 cut(s) 18, 299, 992, 1631, 1633
BshFI GGCC 3 cut(s) 1301, 1414, 1452
BshNI GGYRCC 1 cut(s) 1635
BsiSI CCGG 4 cut(s) 95, 792, 935, 1642
BslFI GGGAC 2 cut(s) 605, 1106
BslI CCNNNNNNNGG 7 cut(s) 191, 782, 785, 1049, 1483, 1606, 1665
BsmAI GTCTC 2 cut(s) 92, 762
BsmBI CGTCTC 1 cut(s) 762
BsmFI GGGAC 2 cut(s) 605, 1106
BsnI GGCC 3 cut(s) 1301, 1414, 1452
Bso31I GGTCTC 1 cut(s) 92
Bsp143I GATC 3 cut(s) 788, 1575, 1776
BspACI CCGC 6 cut(s) 710, 950, 990, 1633, 1748, 1969
BspANI GGCC 3 cut(s) 1301, 1414, 1452
BspCNI CTCAG 5 cut(s) 503, 608, 651, 927, 1139
BspFNI CGCG 5 cut(s) 18, 299, 992, 1631, 1633
BspHI TCATGA 1 cut(s) 1602
BspLI GGNNCC 7 cut(s) 291, 672, 790, 798, 1451, 1542, 1637
BspMI ACCTGC 1 cut(s) 1122
BspPI GGATC 2 cut(s) 783, 796
BspT107I GGYRCC 1 cut(s) 1635
BspTNI GGTCTC 1 cut(s) 92
BsrBI CCGCTC 1 cut(s) 1971
BsrDI GCAATG 2 cut(s) 196, 891
BsrI ACTGG 1 cut(s) 1415
BssECI CCNNGG 3 cut(s) 792, 855, 1043
BssHII GCGCGC 1 cut(s) 16
BssMI GATC 3 cut(s) 788, 1575, 1776
BssNI GRCGYC 1 cut(s) 111
BssT1I CCWWGG 2 cut(s) 855, 1043
Bst2UI CCWGG 2 cut(s) 947, 1401
Bst4CI ACNGT 4 cut(s) 146, 468, 1355, 1742
Bst6I CTCTTC 2 cut(s) 1225, 1314
BstACI GRCGYC 1 cut(s) 111
BstAPI GCANNNNNTGC 1 cut(s) 1114
BstC8I GCNNGC 5 cut(s) 18, 42, 994, 1059, 1759
BstDEI CTNAG 8 cut(s) 158, 490, 595, 659, 914, 1118, 1126, 2007
BstF5I GGATG 4 cut(s) 967, 1009, 1090, 1894
BstFNI CGCG 5 cut(s) 18, 299, 992, 1631, 1633
BstHHI GCGC 3 cut(s) 18, 20, 1633
BstKTI GATC 3 cut(s) 791, 1578, 1779
BstMAI GTCTC 2 cut(s) 92, 762
BstMBI GATC 3 cut(s) 788, 1575, 1776
BstMWI GCNNNNNNNGC 5 cut(s) 772, 989, 1114, 1754, 1909
BstNI CCWGG 2 cut(s) 947, 1401
BstNSI RCATGY 1 cut(s) 726
BstSCI CCNGG 3 cut(s) 791, 945, 1399
BstSFI CTRYAG 3 cut(s) 714, 1014, 1191
BstUI CGCG 5 cut(s) 18, 299, 992, 1631, 1633
BstV1I GCAGC 2 cut(s) 816, 995
BstV2I GAAGAC 4 cut(s) 323, 822, 1661, 1796
BstX2I RGATCY 1 cut(s) 788
BstYI RGATCY 1 cut(s) 788
BsuRI GGCC 3 cut(s) 1301, 1414, 1452
BtgZI GCGATG 1 cut(s) 747
BtrI CACGTC 3 cut(s) 105, 538, 1663
BtsCI GGATG 4 cut(s) 967, 1009, 1090, 1894
BveI ACCTGC 1 cut(s) 1122
Cac8I GCNNGC 5 cut(s) 18, 42, 994, 1059, 1759
CciI TCATGA 1 cut(s) 1602
CfoI GCGC 3 cut(s) 18, 20, 1633
Cfr13I GGNCC 3 cut(s) 350, 707, 1450
CseI GACGC 2 cut(s) 119, 288
Csp6I GTAC 3 cut(s) 899, 976, 1490
CviQI GTAC 3 cut(s) 899, 976, 1490
DdeI CTNAG 8 cut(s) 158, 490, 595, 659, 914, 1118, 1126, 2007
DpnI GATC 3 cut(s) 790, 1577, 1778
DpnII GATC 3 cut(s) 788, 1575, 1776
DraI TTTAAA 1 cut(s) 583
Eam1104I CTCTTC 2 cut(s) 1225, 1314
EarI CTCTTC 2 cut(s) 1225, 1314
EciI GGCGGA 3 cut(s) 699, 965, 1763
Eco130I CCWWGG 2 cut(s) 855, 1043
Eco31I GGTCTC 1 cut(s) 92
Eco47I GGWCC 2 cut(s) 350, 707
EcoO109I RGGNCCY 1 cut(s) 1450
EcoRII CCWGG 2 cut(s) 945, 1399
EcoT14I CCWWGG 2 cut(s) 855, 1043
EcoT22I ATGCAT 1 cut(s) 439
ErhI CCWWGG 2 cut(s) 855, 1043
Esp3I CGTCTC 1 cut(s) 762
FalI AAGNNNNNCTT 2 cut(s) 1830, 1862
FaqI GGGAC 2 cut(s) 605, 1106
FbaI TGATCA 2 cut(s) 1575, 1776
Fnu4HI GCNGC 3 cut(s) 805, 984, 1634
FokI GGATG 4 cut(s) 974, 1016, 1097, 1901
Fsp4HI GCNGC 3 cut(s) 805, 984, 1634
FspBI CTAG 4 cut(s) 6, 369, 675, 1794
GlaI GCGC 3 cut(s) 17, 19, 1632
GluI GCNGC 3 cut(s) 805, 984, 1634
GsaI CCCAGC 1 cut(s) 1765
HaeIII GGCC 3 cut(s) 1301, 1414, 1452
HapII CCGG 4 cut(s) 95, 792, 935, 1642
HgaI GACGC 2 cut(s) 119, 288
HhaI GCGC 3 cut(s) 18, 20, 1633
Hin1I GRCGYC 1 cut(s) 111
Hin6I GCGC 3 cut(s) 16, 18, 1631
HinP1I GCGC 3 cut(s) 16, 18, 1631
HincII GTYRAC 1 cut(s) 1698
HindII GTYRAC 1 cut(s) 1698
HindIII AAGCTT 2 cut(s) 325, 1480
HinfI GANTC 2 cut(s) 593, 965
HpaII CCGG 4 cut(s) 95, 792, 935, 1642
HphI GGTGA 5 cut(s) 1064, 1611, 1777, 1841, 1853
Hpy166II GTNNAC 5 cut(s) 978, 1033, 1698, 1768, 1844
Hpy188I TCNGA 9 cut(s) 109, 151, 180, 403, 598, 814, 917, 1339, 1506
Hpy188III TCNNGA 7 cut(s) 331, 428, 457, 1201, 1526, 1603, 1675
Hpy8I GTNNAC 5 cut(s) 978, 1033, 1698, 1768, 1844
Hpy99I CGWCG 4 cut(s) 113, 304, 539, 1664
HpyAV CCTTC 7 cut(s) 251, 513, 819, 1251, 1308, 1440, 1893
HpyCH4III ACNGT 4 cut(s) 146, 468, 1355, 1742
HpyCH4IV ACGT 4 cut(s) 104, 278, 537, 1662
HpyCH4V TGCA 9 cut(s) 189, 437, 686, 752, 804, 1108, 1393, 1912, 1925
HpyF10VI GCNNNNNNNGC 5 cut(s) 772, 989, 1114, 1754, 1909
HpyF3I CTNAG 8 cut(s) 158, 490, 595, 659, 914, 1118, 1126, 2007
HpySE526I ACGT 4 cut(s) 104, 278, 537, 1662
Hsp92I GRCGYC 1 cut(s) 111
HspAI GCGC 3 cut(s) 16, 18, 1631
Ksp22I TGATCA 2 cut(s) 1575, 1776
Kzo9I GATC 3 cut(s) 788, 1575, 1776
LmnI GCTCC 5 cut(s) 670, 745, 751, 882, 1540
Lsp1109I GCAGC 2 cut(s) 816, 995
LweI GCATC 3 cut(s) 761, 994, 1177
MaeI CTAG 4 cut(s) 6, 369, 675, 1794
MaeII ACGT 4 cut(s) 104, 278, 537, 1662
MaeIII GTNAC 4 cut(s) 194, 267, 1617, 1829
MalI GATC 3 cut(s) 790, 1577, 1778
MbiI CCGCTC 1 cut(s) 1971
MboI GATC 3 cut(s) 788, 1575, 1776
MflI RGATCY 1 cut(s) 788
MluCI AATT 8 cut(s) 122, 163, 205, 337, 445, 518, 602, 938
MlyI GAGTC 1 cut(s) 587
MmeI TCCRAC 5 cut(s) 132, 615, 837, 939, 1668
Mph1103I ATGCAT 1 cut(s) 439
MseI TTAA 3 cut(s) 119, 323, 582
MslI CAYNNNNRTG 1 cut(s) 1374
MspI CCGG 4 cut(s) 95, 792, 935, 1642
MspR9I CCNGG 3 cut(s) 793, 947, 1401
MvaI CCWGG 2 cut(s) 947, 1401
MvnI CGCG 5 cut(s) 18, 299, 992, 1631, 1633
MwoI GCNNNNNNNGC 5 cut(s) 772, 989, 1114, 1754, 1909
NciI CCSGG 1 cut(s) 793
NdeII GATC 3 cut(s) 788, 1575, 1776
NlaIV GGNNCC 7 cut(s) 291, 672, 790, 798, 1451, 1542, 1637
NmuCI GTSAC 2 cut(s) 1617, 1829
NsiI ATGCAT 1 cut(s) 439
NspI RCATGY 1 cut(s) 726
PagI TCATGA 1 cut(s) 1602
PauI GCGCGC 1 cut(s) 16
PfeI GAWTC 1 cut(s) 965
PflFI GACNNNGTC 1 cut(s) 103
PflMI CCANNNNNTGG 1 cut(s) 191
PkrI GCNGC 3 cut(s) 806, 985, 1635
PleI GAGTC 1 cut(s) 587
PpsI GAGTC 1 cut(s) 587
Psp1406I AACGTT 1 cut(s) 278
Psp6I CCWGG 2 cut(s) 945, 1399
PspFI CCCAGC 1 cut(s) 1761
PspGI CCWGG 2 cut(s) 945, 1399
PspN4I GGNNCC 7 cut(s) 291, 672, 790, 798, 1451, 1542, 1637
PspPI GGNCC 3 cut(s) 350, 707, 1450
PsuI RGATCY 1 cut(s) 788
PsyI GACNNNGTC 1 cut(s) 103
PteI GCGCGC 1 cut(s) 16
RsaI GTAC 3 cut(s) 900, 977, 1491
RsaNI GTAC 3 cut(s) 899, 976, 1490
RseI CAYNNNNRTG 1 cut(s) 1374
SaqAI TTAA 3 cut(s) 119, 323, 582
SatI GCNGC 3 cut(s) 805, 984, 1634
Sau3AI GATC 3 cut(s) 788, 1575, 1776
Sau96I GGNCC 3 cut(s) 350, 707, 1450
SchI GAGTC 1 cut(s) 587
ScrFI CCNGG 3 cut(s) 793, 947, 1401
SfaNI GCATC 3 cut(s) 761, 994, 1177
SfcI CTRYAG 3 cut(s) 714, 1014, 1191
SinI GGWCC 2 cut(s) 350, 707
SmiMI CAYNNNNRTG 1 cut(s) 1374
SmlI CTYRAG 1 cut(s) 1972
SmoI CTYRAG 1 cut(s) 1972
SpeI ACTAGT 1 cut(s) 1793
Sse9I AATT 8 cut(s) 122, 163, 205, 337, 445, 518, 602, 938
SsiI CCGC 6 cut(s) 710, 950, 990, 1633, 1748, 1969
SspI AATATT 1 cut(s) 1137
SspMI CTAG 4 cut(s) 6, 369, 675, 1794
StyD4I CCNGG 3 cut(s) 791, 945, 1399
StyI CCWWGG 2 cut(s) 855, 1043
TaaI ACNGT 4 cut(s) 146, 468, 1355, 1742
TaiI ACGT 4 cut(s) 107, 281, 540, 1665
TaqI TCGA 2 cut(s) 255, 534
TaqII GACCGA 1 cut(s) 1673
TasI AATT 8 cut(s) 122, 163, 205, 337, 445, 518, 602, 938
TatI WGTACW 2 cut(s) 898, 975
TauI GCSGC 1 cut(s) 1636
TfiI GAWTC 1 cut(s) 965
Tru1I TTAA 3 cut(s) 119, 323, 582
Tru9I TTAA 3 cut(s) 119, 323, 582
TseFI GTSAC 2 cut(s) 1617, 1829
TseI GCWGC 2 cut(s) 804, 983
Tsp45I GTSAC 2 cut(s) 1617, 1829
TspGWI ACGGA 2 cut(s) 342, 1785
Tth111I GACNNNGTC 1 cut(s) 103
Van91I CCANNNNNTGG 1 cut(s) 191
VpaK11BI GGWCC 2 cut(s) 350, 707
XapI RAATTY 2 cut(s) 122, 445
XceI RCATGY 1 cut(s) 726
XcmI CCANNNNNNNNNTGG 1 cut(s) 1522
XspI CTAG 4 cut(s) 6, 369, 675, 1794
Zsp2I ATGCAT 1 cut(s) 439
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.