Rmu_sc0007451.1_g000001

Dihydrofolate reductase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0007451.1
OG0008449
Physical Location & Seq
Reverse (-)
484 .. 2388
1905 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 969 bp
atgtcatccaacgcatcagccaccctccacttaaaccgcacgcccattctggaccgtcgatttcagctacgcggtctacgcaccctatttattgttccccattcccttcactccattcgcattgagagcaggaaactccaacacgacgtcgttgcgaccaaggccgtgccttttaaatcccccaccacctcccgcactagcggaactgacaagaacaccaacagcctcaacctttccgaaacgacaaggaggaacaccaacaccaacaccaacaccaacaacatgggaagcgagggttgtgccgcggcttctgattcgaggaaacccaggtttctttgccttcatgggtttcgaaccagcggtgtgatcatgaagacccaactgggtaaatggccggagtcggtgctcaacaatttcgacctggactttctcgatgggcctttcccggcccaaggtaaatccgaagtcgagggcattttcgatcctccttatcacgagtggttccaattcagcaaggatttttctgagtacacgaactttgacgagtgtcttgaattcattgaagattacatgatcaagaatggaccctttgatgggctacttggcttttcccagggggcaatactgtcagcggcactgccgggtctacaagcaaagggtttggcactgaccaaagtacctaaaattaaattcctcataataattggaggcgcaaaattcaagtcagcaaccgtggctgaaaatgcttattcatctcctattcaaattccgtctcttcactttttaggagaaacagacttcttgaagccatatggactcgaactgctagaaaactgtgtagatcctaccgtgattcatcatcccaagggccacacgataccacgactcgatgaaaagggtttagagaccattatgagcttcattgagacaattcagaagaagttggacgagaaggaggagcaacagtaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
FSH1

Protein Analysis

322

Amino Acids

35.95

Weight (kDa)

6.8

Isoelectric Point (pI)

35.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FSH1 PF03959 107 - 298 4.8e-53 Serine hydrolase (FSH1)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 150
AccI GTMKAC 2 cut(s) 76, 646
AccII CGCG 2 cut(s) 72, 305
AciI CCGC 8 cut(s) 37, 72, 193, 201, 303, 305, 360, 632
AclWI GGATC 2 cut(s) 476, 836
AcoI YGGCCR 1 cut(s) 392
AcsI RAATTY 4 cut(s) 554, 689, 716, 765
AcyI GRCGYC 1 cut(s) 147
AfaI GTAC 2 cut(s) 530, 678
AfiI CCNNNNNNNGG 3 cut(s) 452, 593, 594
AgsI TTSAA 5 cut(s) 554, 563, 721, 764, 805
AjnI CCWGG 3 cut(s) 326, 420, 612
AluBI AGCT 2 cut(s) 67, 918
AluI AGCT 2 cut(s) 67, 918
Alw21I GWGCWC 1 cut(s) 408
Alw26I GTCTC 3 cut(s) 777, 899, 920
AlwI GGATC 2 cut(s) 476, 836
AoxI GGCC 5 cut(s) 162, 392, 437, 447, 868
ApoI RAATTY 4 cut(s) 554, 689, 716, 765
ArsI GACNNNNNNTTYG 2 cut(s) 707, 739
AspLEI GCGC 1 cut(s) 713
AspS9I GGNCC 5 cut(s) 52, 437, 448, 584, 868
AsuC2I CCSGG 2 cut(s) 446, 642
AsuII TTCGAA 1 cut(s) 352
AvaII GGWCC 2 cut(s) 52, 584
BaeI ACNNNNGTAYC 2 cut(s) 660, 693
BauI CACGAG 1 cut(s) 494
BbsI GAAGAC 1 cut(s) 380
Bbv12I GWGCWC 1 cut(s) 408
BccI CCATC 2 cut(s) 428, 587
BceAI ACGGC 1 cut(s) 149
BcgI CGANNNNNNTGC 4 cut(s) 134, 168, 281, 315
BciT130I CCWGG 3 cut(s) 328, 422, 614
BclI TGATCA 2 cut(s) 366, 573
BcnI CCSGG 2 cut(s) 446, 642
BcoDI GTCTC 3 cut(s) 777, 899, 920
BfaI CTAG 2 cut(s) 198, 827
BisI GCNGC 3 cut(s) 303, 306, 633
BlsI GCNGC 3 cut(s) 304, 307, 634
Bme1390I CCNGG 5 cut(s) 328, 422, 446, 614, 642
Bme18I GGWCC 2 cut(s) 52, 584
BmgT120I GGNCC 5 cut(s) 52, 437, 448, 584, 868
BmiI GGNNCC 2 cut(s) 503, 586
BmrFI CCNGG 5 cut(s) 328, 422, 446, 614, 642
BmrI ACTGGG 1 cut(s) 392
BmsI GCATC 1 cut(s) 23
BmuI ACTGGG 1 cut(s) 392
BoxI GACNNNNGTC 1 cut(s) 546
BpiI GAAGAC 1 cut(s) 380
Bpu14I TTCGAA 1 cut(s) 352
BpuMI CCSGG 2 cut(s) 446, 642
BsaHI GRCGYC 1 cut(s) 147
BsaI GGTCTC 1 cut(s) 899
BsaJI CCNNGG 8 cut(s) 159, 303, 326, 451, 612, 613, 732, 864
BsaXI ACNNNNNCTCC 1 cut(s) 950
Bsc4I CCNNNNNNNGG 3 cut(s) 452, 593, 594
Bse1I ACTGG 1 cut(s) 387
BseBI CCWGG 3 cut(s) 328, 422, 614
BseDI CCNNGG 8 cut(s) 159, 303, 326, 451, 612, 613, 732, 864
BseGI GGATG 2 cut(s) 5, 859
BseLI CCNNNNNNNGG 3 cut(s) 452, 593, 594
BseMII CTCAG 1 cut(s) 516
BseNI ACTGG 1 cut(s) 387
Bsh1236I CGCG 2 cut(s) 72, 305
BshFI GGCC 5 cut(s) 164, 394, 439, 449, 870
BsiHKAI GWGCWC 1 cut(s) 408
BsiSI CCGG 3 cut(s) 395, 446, 641
BslI CCNNNNNNNGG 3 cut(s) 452, 593, 594
BsmAI GTCTC 3 cut(s) 777, 899, 920
BsmBI CGTCTC 1 cut(s) 777
BsnI GGCC 5 cut(s) 164, 394, 439, 449, 870
Bso31I GGTCTC 1 cut(s) 899
Bsp119I TTCGAA 1 cut(s) 352
Bsp1286I GDGCHC 1 cut(s) 408
Bsp143I GATC 4 cut(s) 366, 481, 573, 841
BspACI CCGC 8 cut(s) 37, 72, 193, 201, 303, 305, 360, 632
BspANI GGCC 5 cut(s) 164, 394, 439, 449, 870
BspCNI CTCAG 1 cut(s) 517
BspFNI CGCG 2 cut(s) 72, 305
BspHI TCATGA 1 cut(s) 369
BspLI GGNNCC 2 cut(s) 503, 586
BspPI GGATC 2 cut(s) 476, 836
BspT104I TTCGAA 1 cut(s) 352
BspTNI GGTCTC 1 cut(s) 899
BsrI ACTGG 1 cut(s) 387
BssECI CCNNGG 8 cut(s) 159, 303, 326, 451, 612, 613, 732, 864
BssMI GATC 4 cut(s) 366, 481, 573, 841
BssNI GRCGYC 1 cut(s) 147
BssSI CACGAG 1 cut(s) 494
BssT1I CCWWGG 3 cut(s) 159, 451, 864
Bst2BI CACGAG 1 cut(s) 494
Bst2UI CCWGG 3 cut(s) 328, 422, 614
Bst4CI ACNGT 6 cut(s) 56, 627, 733, 836, 850, 966
Bst6I CTCTTC 1 cut(s) 780
BstACI GRCGYC 1 cut(s) 147
BstBI TTCGAA 1 cut(s) 352
BstC8I GCNNGC 1 cut(s) 41
BstDEI CTNAG 1 cut(s) 525
BstDSI CCRYGG 2 cut(s) 303, 732
BstF5I GGATG 2 cut(s) 5, 859
BstFNI CGCG 2 cut(s) 72, 305
BstHHI GCGC 1 cut(s) 713
BstKTI GATC 4 cut(s) 369, 484, 576, 844
BstMAI GTCTC 3 cut(s) 777, 899, 920
BstMBI GATC 4 cut(s) 366, 481, 573, 841
BstMWI GCNNNNNNNGC 5 cut(s) 78, 126, 161, 734, 743
BstNI CCWGG 3 cut(s) 328, 422, 614
BstPAI GACNNNNGTC 1 cut(s) 546
BstSCI CCNGG 5 cut(s) 326, 420, 444, 612, 640
BstUI CGCG 2 cut(s) 72, 305
BstV2I GAAGAC 1 cut(s) 380
BstX2I RGATCY 1 cut(s) 841
BstXI CCANNNNNNTGG 1 cut(s) 283
BstYI RGATCY 1 cut(s) 841
BsuRI GGCC 5 cut(s) 164, 394, 439, 449, 870
BtgI CCRYGG 2 cut(s) 303, 732
BtsCI GGATG 2 cut(s) 5, 859
BtsI GCAGTG 1 cut(s) 635
BtsIMutI CAGTG 2 cut(s) 635, 665
Cac8I GCNNGC 1 cut(s) 41
CciI TCATGA 1 cut(s) 369
CfoI GCGC 1 cut(s) 713
Cfr13I GGNCC 5 cut(s) 52, 437, 448, 584, 868
Cfr42I CCGCGG 1 cut(s) 306
Csp6I GTAC 2 cut(s) 529, 677
CviAII CATG 4 cut(s) 283, 344, 370, 571
CviQI GTAC 2 cut(s) 529, 677
DdeI CTNAG 1 cut(s) 525
DpnI GATC 4 cut(s) 368, 483, 575, 843
DpnII GATC 4 cut(s) 366, 481, 573, 841
DraI TTTAAA 1 cut(s) 175
EaeI YGGCCR 1 cut(s) 392
Eam1104I CTCTTC 1 cut(s) 780
EarI CTCTTC 1 cut(s) 780
Eco130I CCWWGG 3 cut(s) 159, 451, 864
Eco31I GGTCTC 1 cut(s) 899
Eco47I GGWCC 2 cut(s) 52, 584
EcoRI GAATTC 1 cut(s) 554
EcoRII CCWGG 3 cut(s) 326, 420, 612
EcoT14I CCWWGG 3 cut(s) 159, 451, 864
ErhI CCWWGG 3 cut(s) 159, 451, 864
Esp3I CGTCTC 1 cut(s) 777
FaeI CATG 4 cut(s) 286, 347, 373, 574
FaiI YATR 8 cut(s) 284, 345, 371, 572, 698, 811, 813, 914
FatI CATG 4 cut(s) 282, 343, 369, 570
FauI CCCGC 1 cut(s) 200
FauNDI CATATG 1 cut(s) 811
FbaI TGATCA 2 cut(s) 366, 573
FblI GTMKAC 2 cut(s) 76, 646
Fnu4HI GCNGC 3 cut(s) 303, 306, 633
FokI GGATG 1 cut(s) 846
Fsp4HI GCNGC 3 cut(s) 303, 306, 633
FspBI CTAG 2 cut(s) 198, 827
GlaI GCGC 1 cut(s) 712
GluI GCNGC 3 cut(s) 303, 306, 633
HaeIII GGCC 5 cut(s) 164, 394, 439, 449, 870
HapII CCGG 3 cut(s) 395, 446, 641
HhaI GCGC 1 cut(s) 713
Hin1I GRCGYC 1 cut(s) 147
Hin1II CATG 4 cut(s) 286, 347, 373, 574
Hin6I GCGC 1 cut(s) 711
HinP1I GCGC 1 cut(s) 711
HinfI GANTC 5 cut(s) 314, 398, 816, 853, 885
HpaII CCGG 3 cut(s) 395, 446, 641
Hpy166II GTNNAC 3 cut(s) 77, 531, 647
Hpy188I TCNGA 5 cut(s) 238, 313, 463, 526, 936
Hpy188III TCNNGA 7 cut(s) 50, 370, 431, 494, 551, 577, 802
Hpy8I GTNNAC 3 cut(s) 77, 531, 647
Hpy99I CGWCG 3 cut(s) 60, 149, 152
HpyAV CCTTC 3 cut(s) 116, 350, 946
HpyCH4III ACNGT 6 cut(s) 56, 627, 733, 836, 850, 966
HpyCH4IV ACGT 1 cut(s) 147
HpyF10VI GCNNNNNNNGC 5 cut(s) 78, 126, 161, 734, 743
HpyF3I CTNAG 1 cut(s) 525
HpySE526I ACGT 1 cut(s) 147
Hsp92I GRCGYC 1 cut(s) 147
Hsp92II CATG 4 cut(s) 286, 347, 373, 574
HspAI GCGC 1 cut(s) 711
Ksp22I TGATCA 2 cut(s) 366, 573
KspI CCGCGG 1 cut(s) 306
Kzo9I GATC 4 cut(s) 366, 481, 573, 841
LmnI GCTCC 1 cut(s) 958
LweI GCATC 1 cut(s) 23
MaeI CTAG 2 cut(s) 198, 827
MaeII ACGT 1 cut(s) 147
MalI GATC 4 cut(s) 368, 483, 575, 843
MboI GATC 4 cut(s) 366, 481, 573, 841
MboII GAAGA 4 cut(s) 385, 575, 767, 949
MflI RGATCY 1 cut(s) 841
MhlI GDGCHC 1 cut(s) 408
MluCI AATT 9 cut(s) 412, 506, 554, 684, 689, 702, 716, 765, 930
MlyI GAGTC 3 cut(s) 407, 810, 879
MmeI TCCRAC 3 cut(s) 33, 163, 924
MseI TTAA 3 cut(s) 32, 174, 687
MspA1I CMGCKG 3 cut(s) 305, 360, 632
MspI CCGG 3 cut(s) 395, 446, 641
MspR9I CCNGG 5 cut(s) 328, 422, 446, 614, 642
MvaI CCWGG 3 cut(s) 328, 422, 614
MvnI CGCG 2 cut(s) 72, 305
MwoI GCNNNNNNNGC 5 cut(s) 78, 126, 161, 734, 743
NciI CCSGG 2 cut(s) 446, 642
NdeI CATATG 1 cut(s) 811
NdeII GATC 4 cut(s) 366, 481, 573, 841
NlaIII CATG 4 cut(s) 286, 347, 373, 574
NlaIV GGNNCC 2 cut(s) 503, 586
NspV TTCGAA 1 cut(s) 352
PagI TCATGA 1 cut(s) 369
PasI CCCWGGG 1 cut(s) 613
PfeI GAWTC 2 cut(s) 314, 853
PkrI GCNGC 3 cut(s) 304, 307, 634
PleI GAGTC 3 cut(s) 406, 810, 879
PpsI GAGTC 3 cut(s) 406, 810, 879
PshAI GACNNNNGTC 1 cut(s) 546
Psp6I CCWGG 3 cut(s) 326, 420, 612
PspGI CCWGG 3 cut(s) 326, 420, 612
PspN4I GGNNCC 2 cut(s) 503, 586
PspPI GGNCC 5 cut(s) 52, 437, 448, 584, 868
PsuI RGATCY 1 cut(s) 841
RsaI GTAC 2 cut(s) 530, 678
RsaNI GTAC 2 cut(s) 529, 677
SacII CCGCGG 1 cut(s) 306
SaqAI TTAA 3 cut(s) 32, 174, 687
SatI GCNGC 3 cut(s) 303, 306, 633
Sau3AI GATC 4 cut(s) 366, 481, 573, 841
Sau96I GGNCC 5 cut(s) 52, 437, 448, 584, 868
SchI GAGTC 3 cut(s) 407, 810, 879
ScrFI CCNGG 5 cut(s) 328, 422, 446, 614, 642
SduI GDGCHC 1 cut(s) 408
SetI ASST 9 cut(s) 69, 150, 191, 234, 332, 423, 457, 682, 920
SfaNI GCATC 1 cut(s) 23
Sfr303I CCGCGG 1 cut(s) 306
SfuI TTCGAA 1 cut(s) 352
SgrBI CCGCGG 1 cut(s) 306
SinI GGWCC 2 cut(s) 52, 584
Sse9I AATT 9 cut(s) 412, 506, 554, 684, 689, 702, 716, 765, 930
SsiI CCGC 8 cut(s) 37, 72, 193, 201, 303, 305, 360, 632
SspMI CTAG 2 cut(s) 198, 827
StyD4I CCNGG 5 cut(s) 326, 420, 444, 612, 640
StyI CCWWGG 3 cut(s) 159, 451, 864
TaaI ACNGT 6 cut(s) 56, 627, 733, 836, 850, 966
TaiI ACGT 1 cut(s) 150
TaqI TCGA 9 cut(s) 58, 317, 352, 417, 432, 468, 480, 819, 888
TasI AATT 9 cut(s) 412, 506, 554, 684, 689, 702, 716, 765, 930
TatI WGTACW 1 cut(s) 528
TauI GCSGC 3 cut(s) 305, 308, 635
TfiI GAWTC 2 cut(s) 314, 853
Tru1I TTAA 3 cut(s) 32, 174, 687
Tru9I TTAA 3 cut(s) 32, 174, 687
TscAI CASTG 2 cut(s) 642, 672
TspDTI ATGAA 7 cut(s) 332, 386, 547, 741, 845, 906, 910
TspGWI ACGGA 1 cut(s) 759
TspRI CASTG 2 cut(s) 642, 672
VpaK11BI GGWCC 2 cut(s) 52, 584
XapI RAATTY 4 cut(s) 554, 689, 716, 765
XmiI GTMKAC 2 cut(s) 76, 646
XspI CTAG 2 cut(s) 198, 827
ZraI GACGTC 1 cut(s) 148
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.