Rmu_ssc0000067.1_g000050

Dihydrofolate reductase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_ssc0000067.1
OG0008449
Physical Location & Seq
Forward (+)
190332 .. 191078
747 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 663 bp
atgtcatccaacgcatcatccaccctccacttaaaccgcacgcccattctggaccgtcgatttcagctacgcggtctacgcaccctatttattgttccccattcccttcaccccattcgcattgagagcaggaaactccaacacgacgtcgttgcgaccaaggccgtgccttttaaatcccccaccacctcccgcactagcggaactgacaagaacaccaacagcctcaacctttccgaaacgacaaggaggaacaccaacgccaacactaacaacatgggaagcgagggttgtgccgcggcttctgattcggggaaacccaggtttctttgccttcaggggtttcgaaccagcggtgtgatcatgaagactcaaatgcgtaaatggccggagtcggtgctcaacaatttcgacctggactttctcgatgggccgttcccggcccaaggtaaatccgaagtcgagggcattttcgatcctccttatcacgagtggttccaattcagcaaggatttttctgagtacacgaactttgacgagtgtcttgaattcattgaagattacatgatcaagaatggaccctttgatgggctacttggcttttcccaggtatttttctacttattagcgctttattctcttcctgggttcctcaagaaatga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
FSH1

Protein Analysis

220

Amino Acids

24.97

Weight (kDa)

8.42

Isoelectric Point (pI)

44.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FSH1 PF03959 106 - 203 6.8e-25 Serine hydrolase (FSH1)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 150
AccI GTMKAC 1 cut(s) 76
AccII CGCG 2 cut(s) 72, 299
AciI CCGC 7 cut(s) 37, 72, 193, 201, 297, 299, 354
AclWI GGATC 1 cut(s) 470
AcoI YGGCCR 1 cut(s) 386
AcsI RAATTY 1 cut(s) 548
AcuI CTGAAG 1 cut(s) 320
AcyI GRCGYC 1 cut(s) 147
AfaI GTAC 1 cut(s) 524
AfeI AGCGCT 1 cut(s) 630
AfiI CCNNNNNNNGG 3 cut(s) 446, 587, 588
AgsI TTSAA 2 cut(s) 548, 557
AjnI CCWGG 4 cut(s) 320, 414, 606, 643
AluBI AGCT 1 cut(s) 67
AluI AGCT 1 cut(s) 67
Alw21I GWGCWC 1 cut(s) 402
AlwI GGATC 1 cut(s) 470
Aor51HI AGCGCT 1 cut(s) 630
AoxI GGCC 4 cut(s) 162, 386, 431, 441
ApoI RAATTY 1 cut(s) 548
AspLEI GCGC 1 cut(s) 631
AspS9I GGNCC 4 cut(s) 52, 431, 442, 578
AsuC2I CCSGG 1 cut(s) 440
AsuHPI GGTGA 1 cut(s) 101
AsuII TTCGAA 1 cut(s) 346
AvaII GGWCC 2 cut(s) 52, 578
BauI CACGAG 1 cut(s) 488
BbsI GAAGAC 1 cut(s) 374
Bbv12I GWGCWC 1 cut(s) 402
BccI CCATC 2 cut(s) 422, 581
BceAI ACGGC 2 cut(s) 149, 418
BcgI CGANNNNNNTGC 4 cut(s) 134, 168, 275, 309
BciT130I CCWGG 4 cut(s) 322, 416, 608, 645
BclI TGATCA 2 cut(s) 360, 567
BcnI CCSGG 1 cut(s) 440
BfaI CTAG 1 cut(s) 198
BfoI RGCGCY 1 cut(s) 632
BisI GCNGC 2 cut(s) 297, 300
BlsI GCNGC 2 cut(s) 298, 301
Bme1390I CCNGG 5 cut(s) 322, 416, 440, 608, 645
Bme18I GGWCC 2 cut(s) 52, 578
BmgT120I GGNCC 4 cut(s) 52, 431, 442, 578
BmiI GGNNCC 3 cut(s) 497, 580, 650
BmrFI CCNGG 5 cut(s) 322, 416, 440, 608, 645
BmsI GCATC 1 cut(s) 23
BoxI GACNNNNGTC 1 cut(s) 540
BpiI GAAGAC 1 cut(s) 374
Bpu14I TTCGAA 1 cut(s) 346
BpuEI CTTGAG 1 cut(s) 638
BpuMI CCSGG 1 cut(s) 440
BsaHI GRCGYC 1 cut(s) 147
BsaJI CCNNGG 6 cut(s) 159, 297, 320, 445, 606, 644
Bsc4I CCNNNNNNNGG 3 cut(s) 446, 587, 588
BseBI CCWGG 4 cut(s) 322, 416, 608, 645
BseDI CCNNGG 6 cut(s) 159, 297, 320, 445, 606, 644
BseGI GGATG 2 cut(s) 5, 17
BseLI CCNNNNNNNGG 3 cut(s) 446, 587, 588
BseMII CTCAG 1 cut(s) 510
Bsh1236I CGCG 2 cut(s) 72, 299
BshFI GGCC 4 cut(s) 164, 388, 433, 443
BsiHKAI GWGCWC 1 cut(s) 402
BsiSI CCGG 2 cut(s) 389, 440
BslI CCNNNNNNNGG 3 cut(s) 446, 587, 588
BsnI GGCC 4 cut(s) 164, 388, 433, 443
Bsp119I TTCGAA 1 cut(s) 346
Bsp1286I GDGCHC 1 cut(s) 402
Bsp143I GATC 3 cut(s) 360, 475, 567
BspACI CCGC 7 cut(s) 37, 72, 193, 201, 297, 299, 354
BspANI GGCC 4 cut(s) 164, 388, 433, 443
BspCNI CTCAG 1 cut(s) 511
BspFNI CGCG 2 cut(s) 72, 299
BspHI TCATGA 1 cut(s) 363
BspLI GGNNCC 3 cut(s) 497, 580, 650
BspPI GGATC 1 cut(s) 470
BspT104I TTCGAA 1 cut(s) 346
BssECI CCNNGG 6 cut(s) 159, 297, 320, 445, 606, 644
BssMI GATC 3 cut(s) 360, 475, 567
BssNI GRCGYC 1 cut(s) 147
BssSI CACGAG 1 cut(s) 488
BssT1I CCWWGG 2 cut(s) 159, 445
Bst2BI CACGAG 1 cut(s) 488
Bst2UI CCWGG 4 cut(s) 322, 416, 608, 645
Bst4CI ACNGT 1 cut(s) 56
Bst6I CTCTTC 1 cut(s) 645
BstACI GRCGYC 1 cut(s) 147
BstBI TTCGAA 1 cut(s) 346
BstC8I GCNNGC 1 cut(s) 41
BstDEI CTNAG 1 cut(s) 519
BstDSI CCRYGG 1 cut(s) 297
BstF5I GGATG 2 cut(s) 5, 17
BstFNI CGCG 2 cut(s) 72, 299
BstH2I RGCGCY 1 cut(s) 632
BstHHI GCGC 1 cut(s) 631
BstKTI GATC 3 cut(s) 363, 478, 570
BstMBI GATC 3 cut(s) 360, 475, 567
BstMWI GCNNNNNNNGC 4 cut(s) 78, 126, 161, 385
BstNI CCWGG 4 cut(s) 322, 416, 608, 645
BstPAI GACNNNNGTC 1 cut(s) 540
BstSCI CCNGG 5 cut(s) 320, 414, 438, 606, 643
BstUI CGCG 2 cut(s) 72, 299
BstV2I GAAGAC 1 cut(s) 374
BsuRI GGCC 4 cut(s) 164, 388, 433, 443
BtgI CCRYGG 1 cut(s) 297
BtsCI GGATG 2 cut(s) 5, 17
Cac8I GCNNGC 1 cut(s) 41
CciI TCATGA 1 cut(s) 363
CfoI GCGC 1 cut(s) 631
Cfr13I GGNCC 4 cut(s) 52, 431, 442, 578
Cfr42I CCGCGG 1 cut(s) 300
Csp6I GTAC 1 cut(s) 523
CviAII CATG 3 cut(s) 277, 364, 565
CviJI RGCY 9 cut(s) 67, 164, 225, 302, 388, 433, 443, 592, 600
CviKI_1 RGCY 9 cut(s) 67, 164, 225, 302, 388, 433, 443, 592, 600
CviQI GTAC 1 cut(s) 523
DdeI CTNAG 1 cut(s) 519
DpnI GATC 3 cut(s) 362, 477, 569
DpnII GATC 3 cut(s) 360, 475, 567
DraI TTTAAA 1 cut(s) 175
EaeI YGGCCR 1 cut(s) 386
Eam1104I CTCTTC 1 cut(s) 645
EarI CTCTTC 1 cut(s) 645
Eco130I CCWWGG 2 cut(s) 159, 445
Eco47I GGWCC 2 cut(s) 52, 578
Eco47III AGCGCT 1 cut(s) 630
Eco57I CTGAAG 1 cut(s) 320
EcoRI GAATTC 1 cut(s) 548
EcoRII CCWGG 4 cut(s) 320, 414, 606, 643
EcoT14I CCWWGG 2 cut(s) 159, 445
ErhI CCWWGG 2 cut(s) 159, 445
FaeI CATG 3 cut(s) 280, 367, 568
FaiI YATR 3 cut(s) 278, 365, 566
FatI CATG 3 cut(s) 276, 363, 564
FauI CCCGC 1 cut(s) 200
FbaI TGATCA 2 cut(s) 360, 567
FblI GTMKAC 1 cut(s) 76
Fnu4HI GCNGC 2 cut(s) 297, 300
FokI GGATG 1 cut(s) 4
Fsp4HI GCNGC 2 cut(s) 297, 300
FspBI CTAG 1 cut(s) 198
GlaI GCGC 1 cut(s) 630
GluI GCNGC 2 cut(s) 297, 300
HaeII RGCGCY 1 cut(s) 632
HaeIII GGCC 4 cut(s) 164, 388, 433, 443
HapII CCGG 2 cut(s) 389, 440
HhaI GCGC 1 cut(s) 631
Hin1I GRCGYC 1 cut(s) 147
Hin1II CATG 3 cut(s) 280, 367, 568
Hin6I GCGC 1 cut(s) 629
HinP1I GCGC 1 cut(s) 629
HinfI GANTC 3 cut(s) 308, 370, 392
HpaII CCGG 2 cut(s) 389, 440
HphI GGTGA 1 cut(s) 101
Hpy166II GTNNAC 2 cut(s) 77, 525
Hpy188I TCNGA 4 cut(s) 238, 307, 457, 520
Hpy188III TCNNGA 7 cut(s) 50, 364, 425, 488, 545, 571, 655
Hpy8I GTNNAC 2 cut(s) 77, 525
Hpy99I CGWCG 3 cut(s) 60, 149, 152
HpyAV CCTTC 2 cut(s) 116, 344
HpyCH4III ACNGT 1 cut(s) 56
HpyCH4IV ACGT 1 cut(s) 147
HpyF10VI GCNNNNNNNGC 4 cut(s) 78, 126, 161, 385
HpyF3I CTNAG 1 cut(s) 519
HpySE526I ACGT 1 cut(s) 147
Hsp92I GRCGYC 1 cut(s) 147
Hsp92II CATG 3 cut(s) 280, 367, 568
HspAI GCGC 1 cut(s) 629
Ksp22I TGATCA 2 cut(s) 360, 567
KspI CCGCGG 1 cut(s) 300
Kzo9I GATC 3 cut(s) 360, 475, 567
LweI GCATC 1 cut(s) 23
MaeI CTAG 1 cut(s) 198
MaeII ACGT 1 cut(s) 147
MalI GATC 3 cut(s) 362, 477, 569
MboI GATC 3 cut(s) 360, 475, 567
MboII GAAGA 3 cut(s) 379, 569, 632
MhlI GDGCHC 1 cut(s) 402
MluCI AATT 3 cut(s) 406, 500, 548
MlyI GAGTC 2 cut(s) 364, 401
MmeI TCCRAC 2 cut(s) 33, 163
MnlI CCTC 8 cut(s) 35, 199, 236, 243, 280, 457, 489, 662
MseI TTAA 2 cut(s) 32, 174
MspA1I CMGCKG 2 cut(s) 299, 354
MspI CCGG 2 cut(s) 389, 440
MspR9I CCNGG 5 cut(s) 322, 416, 440, 608, 645
MvaI CCWGG 4 cut(s) 322, 416, 608, 645
MvnI CGCG 2 cut(s) 72, 299
MwoI GCNNNNNNNGC 4 cut(s) 78, 126, 161, 385
NciI CCSGG 1 cut(s) 440
NdeII GATC 3 cut(s) 360, 475, 567
NlaIII CATG 3 cut(s) 280, 367, 568
NlaIV GGNNCC 3 cut(s) 497, 580, 650
NspV TTCGAA 1 cut(s) 346
PagI TCATGA 1 cut(s) 363
PfeI GAWTC 1 cut(s) 308
PkrI GCNGC 2 cut(s) 298, 301
PleI GAGTC 2 cut(s) 364, 400
PpsI GAGTC 2 cut(s) 364, 400
PshAI GACNNNNGTC 1 cut(s) 540
Psp6I CCWGG 4 cut(s) 320, 414, 606, 643
PspGI CCWGG 4 cut(s) 320, 414, 606, 643
PspN4I GGNNCC 3 cut(s) 497, 580, 650
PspPI GGNCC 4 cut(s) 52, 431, 442, 578
RsaI GTAC 1 cut(s) 524
RsaNI GTAC 1 cut(s) 523
SacII CCGCGG 1 cut(s) 300
SaqAI TTAA 2 cut(s) 32, 174
SatI GCNGC 2 cut(s) 297, 300
Sau3AI GATC 3 cut(s) 360, 475, 567
Sau96I GGNCC 4 cut(s) 52, 431, 442, 578
SchI GAGTC 2 cut(s) 364, 401
ScrFI CCNGG 5 cut(s) 322, 416, 440, 608, 645
SduI GDGCHC 1 cut(s) 402
SetI ASST 8 cut(s) 69, 150, 191, 234, 326, 417, 451, 612
SfaNI GCATC 1 cut(s) 23
Sfr303I CCGCGG 1 cut(s) 300
SfuI TTCGAA 1 cut(s) 346
SgrBI CCGCGG 1 cut(s) 300
SinI GGWCC 2 cut(s) 52, 578
SmlI CTYRAG 1 cut(s) 653
SmoI CTYRAG 1 cut(s) 653
Sse9I AATT 3 cut(s) 406, 500, 548
SsiI CCGC 7 cut(s) 37, 72, 193, 201, 297, 299, 354
SspMI CTAG 1 cut(s) 198
StyD4I CCNGG 5 cut(s) 320, 414, 438, 606, 643
StyI CCWWGG 2 cut(s) 159, 445
TaaI ACNGT 1 cut(s) 56
TaiI ACGT 1 cut(s) 150
TaqI TCGA 6 cut(s) 58, 346, 411, 426, 462, 474
TasI AATT 3 cut(s) 406, 500, 548
TatI WGTACW 1 cut(s) 522
TauI GCSGC 2 cut(s) 299, 302
TfiI GAWTC 1 cut(s) 308
Tru1I TTAA 2 cut(s) 32, 174
Tru9I TTAA 2 cut(s) 32, 174
TspDTI ATGAA 2 cut(s) 380, 541
VpaK11BI GGWCC 2 cut(s) 52, 578
XapI RAATTY 1 cut(s) 548
XmiI GTMKAC 1 cut(s) 76
XspI CTAG 1 cut(s) 198
ZraI GACGTC 1 cut(s) 148
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.