Rmu_ssc0000148.1_g000003

LRR receptor-like serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_ssc0000148.1
OG0002722
Physical Location & Seq
Reverse (-)
11599 .. 19870
8272 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 3093 bp
atggctcgtaacaaagcagcagcagcacacaatcaattgctgctatttctgttagcaatagtatcatcatgtgttatctcatcttctagtgctgctagtgaggctgaaacgctcatcaccttcaagaattctctatccgatacctcggcactagataactggaatgcttctacaaaaccctgcaccgggtcccagagccattggacgggtgtgcagtgtaacaacgggagcgtatctgggctgcagctggagaacatgagcctaactggcctgattgaaattgacacgctcacacagttgccggccctcaaggccataagcttcatgaacaacggctttggaggtccactgcccgatgtgaagagactttcactccaaggtctctacttatcccagaaccaactctctggtgatatcccggatgacgctttcagtggcatgggtgataccttgaagaagctttacttggccaaaaatggatttacggggaagataccgagttctttggttgacttgcctaagctcattgagttggaccttggggataaccaatttacaggggagattccatattttcatccaggggatattggacgagagaaaagtcatcatccaccttgctgtgctgcggtgaccagtggccggccgcggactccagcaaccggtgacgggactatggcgatcggtgacctgaatcccgaatccgactatcggactggtgaccggatttcgccccgatgcagcaacacccctgcttgctggtccgtaccagccccatccacgccggcgctctcttccatcacagcaaccatctctcgacgtctccagataggagtaaaccgagaaaacctctgcaccgatcggatgttgggtgcagctgagggatccatggatctaatgacgctccgtttgttcaacaaatccagctgctccaccgagatccgacatcctcatctgcaccaccggcgagttcaccaccaccttcaacaactggctccgactcagcttggacagcacctctacatcaccggtgttgcagaagctgcgtttcaggacaggcagggagtcggcggagttggaggagacgatgggcatggcatagagggcccaaggctggaggaatctaatgtagcagttccgatggcacctagtagggagaaccgatcgaagattaccacttatccggcctccccatatatgcacgaggttgtatttgctagttcatcatgttctgctactgaaggtgacacactcatcaagttcaagacttctctatccgatacctcggcactagataactggaattcttctacaaaaccctgcaacggttccacgagccattgggcgggtgtgctttgtaacggcgggagcgtattcgggctgcagctgcagaacatgggcctaactggcctgattgacattgacacgctctcagagttgccggccctcaagaccataagcttcatgaacaacagctttggaggtccactgcccgatgtgaagagactttcactcattagtgtctacttatcccagaaccaattctctggtgatatccgggatgatgctttcagtggcatgggtgataccttgaagaaagtttacttggccaaaaatggatttacggggaagataccgagttctttggttgatttgcctaagctcattgagttggatcttggggataaccagtttagtgggaagataccaaattttcaaccaaggaactggacgttgttaaatctggcaaacaacagattggatggtcggattccggcatcactaagcaatttagatgcagccgattttcaggcaggaaacaaaggcttgtgtggaccgcctcttggcaagtgcaagtccccaaagaagcgactcctcgtaatcatagccatcatagtcatctcagtggctttactattgtgtgtcttctggataatcaccttaatccggcgcagtcgagctcgtcggacccgtcaagcccaatcggcccaacaggaaaaagcccaaatgaagccggtagcccgtaaaactcgcggagtattggaggcccaattacctgacctaccggtggaagacgatgagtacaaaaaggcagaaaaaggaggggagttgcattttgtgagaaaggacagagagaggtttgaggtgcaagagctacttagggcaccagctgaggttttggggagtgggagctttgggtcgtcttacaaggctggtcttatgagtgggtctatggttgtgaagaggtttagggatatgaatagagttgggagagatgatttctatgatcacatgagtaggattggaaggttgtcacaccctaatctgctcccactcgttgcattctattacatgaaagaagagaagctcttggttcatgactttgctctaaacggaagcttggctagtcatctccatgccaagcgagccccgggacagccaggactcgactggccaacccgcctgatgatcataaaaggagtagcaagaggcctaggttacctctacaaagagttcccaggcctgcctgtcccccacggccacctgaagtcctccaacgtcctcctcgaccacaacctcaacccggtcctagccgaatacgccctaatccctgtcatcaacaaagaccacgcccacaagttcatggtcgcctacaaatcccccgagtactcccaaaccggacgcacctccaagaagaccgacgtgtggagcctcggcatcctcatcttcgaaatgctgaccggaaagttccccgcaaactacctccaacagggcaagagggccaacgccgacctcgccagctgggtcaactcggtggtcagggaggagtggaccggcgaggttttcgataaagacatgaagggtaccaagaatggagaaggcgagatgctgaagctgttgaagatagggatgtgctgctgtgagagtagtgttgaagggagatgggattggagagaggcggtggataagatcgaggagttgaaggagagggacagtgaggatgaagactactcgtcgtacgcgagtgatggggatgtgtactcgtcgagggctgtgactgatgatgacttctccttctcggttgcagcttga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_8 Pkinase Pkinase_Tyr

Protein Analysis

1030

Amino Acids

112.63

Weight (kDa)

7.33

Isoelectric Point (pI)

37.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 32 - 74 5.7e-08 Leucine rich repeat N-terminal domain
LRR_8 PF13855 124 - 185 3.4e-07 Leucine rich repeat
LRRNT_2 PF08263 412 - 454 8.7e-08 Leucine rich repeat N-terminal domain
PK_Tyr_Ser-Thr PF07714 725 - 923 4.5e-24 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 725 - 986 2.1e-22 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 823
Acc65I GGTACC 1 cut(s) 2864
AccB1I GGYRCC 3 cut(s) 1144, 2164, 2864
AccI GTMKAC 1 cut(s) 1524
AccII CGCG 3 cut(s) 649, 2034, 3023
AclWI GGATC 5 cut(s) 879, 892, 900, 934, 1683
AcoI YGGCCR 6 cut(s) 468, 640, 644, 1608, 2453, 2539
AcsI RAATTY 3 cut(s) 127, 1303, 1711
AcuI CTGAAG 3 cut(s) 1260, 2567, 2912
AcyI GRCGYC 1 cut(s) 820
AfaI GTAC 6 cut(s) 768, 2084, 2669, 2866, 3020, 3041
AflIII ACRYGT 1 cut(s) 2703
AgeI ACCGGT 3 cut(s) 662, 1028, 2065
AhdI GACNNNNNGTC 1 cut(s) 3013
AjiI CACGTC 1 cut(s) 2704
AjnI CCWGG 3 cut(s) 580, 2440, 2518
AjuI GAANNNNNNNTTGG 4 cut(s) 449, 481, 1589, 1621
Alw21I GWGCWC 1 cut(s) 1963
Alw26I GTCTC 5 cut(s) 358, 386, 827, 1077, 1498
AlwI GGATC 5 cut(s) 879, 892, 900, 934, 1683
AlwNI CAGNNNCTG 1 cut(s) 1043
Ama87I CYCGRG 2 cut(s) 2431, 2663
ApaI GGGCCC 1 cut(s) 1109
ApoI RAATTY 3 cut(s) 127, 1303, 1711
ArsI GACNNNNNNTTYG 2 cut(s) 892, 924
AsiGI ACCGGT 3 cut(s) 662, 1028, 2065
Asp700I GAANNNNTTC 1 cut(s) 1541
Asp718I GGTACC 1 cut(s) 2864
AspA2I CCTAGG 1 cut(s) 2494
AspLEI GCGC 2 cut(s) 790, 1953
AsuC2I CCSGG 6 cut(s) 187, 419, 1559, 2432, 2433, 2585
AsuII TTCGAA 1 cut(s) 2730
AvaI CYCGRG 2 cut(s) 2431, 2663
AvaII GGWCC 9 cut(s) 189, 344, 535, 762, 1484, 1835, 1968, 2587, 2832
AvrII CCTAGG 1 cut(s) 2494
BaeGI GKGCMC 2 cut(s) 1109, 2167
BalI TGGCCA 3 cut(s) 470, 1610, 2455
BamHI GGATCC 1 cut(s) 884
BanI GGYRCC 3 cut(s) 1144, 2164, 2864
BanII GRGCYC 3 cut(s) 1109, 1963, 2431
BarI GAAGNNNNNNTAC 4 cut(s) 446, 478, 1586, 1618
BauI CACGAG 2 cut(s) 1202, 1333
BbsI GAAGAC 4 cut(s) 1918, 2079, 2702, 3012
Bbv12I GWGCWC 1 cut(s) 1963
BbvCI CCTCAGC 2 cut(s) 879, 2172
BccI CCATC 9 cut(s) 784, 806, 818, 1082, 1135, 1757, 1898, 2937, 3023
BceAI ACGGC 3 cut(s) 349, 1378, 2554
BciT130I CCWGG 3 cut(s) 582, 2442, 2520
BclI TGATCA 2 cut(s) 2287, 2469
BcnI CCSGG 6 cut(s) 187, 419, 1559, 2432, 2433, 2585
BcoDI GTCTC 5 cut(s) 358, 386, 827, 1077, 1498
BfaI CTAG 9 cut(s) 87, 96, 152, 1149, 1218, 1292, 2406, 2495, 2591
BfmI CTRYAG 3 cut(s) 242, 1382, 1388
BfoI RGCGCY 1 cut(s) 791
BglI GCCNNNNNGGC 4 cut(s) 267, 311, 1407, 1985
BlnI CCTAGG 1 cut(s) 2494
BmcAI AGTACT 1 cut(s) 2669
Bme1390I CCNGG 9 cut(s) 187, 419, 582, 1559, 2432, 2433, 2442, 2520, 2585
Bme18I GGWCC 9 cut(s) 189, 344, 535, 762, 1484, 1835, 1968, 2587, 2832
BmeRI GACNNNNNGTC 1 cut(s) 3013
BmeT110I CYCGRG 2 cut(s) 2431, 2663
BmgBI CACGTC 1 cut(s) 2704
BmrFI CCNGG 9 cut(s) 187, 419, 582, 1559, 2432, 2433, 2442, 2520, 2585
BmsI GCATC 6 cut(s) 728, 1555, 1786, 1787, 2727, 2877
BpiI GAAGAC 4 cut(s) 1918, 2079, 2702, 3012
BplI GAGNNNNNCTC 2 cut(s) 834, 866
BpmI CTGGAG 4 cut(s) 269, 639, 809, 1136
Bpu10I CCTNAGC 4 cut(s) 519, 879, 1659, 2172
Bpu14I TTCGAA 1 cut(s) 2730
BpuEI CTTGAG 2 cut(s) 293, 1433
BpuMI CCSGG 6 cut(s) 187, 419, 1559, 2432, 2433, 2585
BsaHI GRCGYC 1 cut(s) 820
BsaI GGTCTC 1 cut(s) 386
BsaWI WCCGGW 6 cut(s) 662, 723, 1028, 2065, 2678, 2741
BsaXI ACNNNNNCTCC 2 cut(s) 357, 387
BseBI CCWGG 3 cut(s) 582, 2442, 2520
BseMII CTCAG 5 cut(s) 870, 1016, 1446, 1917, 2163
BseRI GAGGAG 5 cut(s) 1095, 1865, 2555, 2840, 2990
BseSI GKGCMC 2 cut(s) 1109, 2167
BseX3I CGGCCG 1 cut(s) 644
BseYI CCCAGC 1 cut(s) 2802
BsgI GTGCAG 5 cut(s) 166, 233, 838, 894, 941
Bsh1236I CGCG 3 cut(s) 649, 2034, 3023
Bsh1285I CGRYCG 4 cut(s) 647, 684, 862, 1166
BshNI GGYRCC 3 cut(s) 1144, 2164, 2864
BshTI ACCGGT 3 cut(s) 662, 1028, 2065
BsiEI CGRYCG 4 cut(s) 647, 684, 862, 1166
BsiHKAI GWGCWC 1 cut(s) 1963
BsiHKCI CYCGRG 2 cut(s) 2431, 2663
BsiWI CGTACG 1 cut(s) 3018
BslFI GGGAC 6 cut(s) 175, 685, 1843, 2448, 2516, 3005
BsmAI GTCTC 5 cut(s) 358, 386, 827, 1077, 1498
BsmBI CGTCTC 2 cut(s) 827, 1077
BsmFI GGGAC 6 cut(s) 175, 685, 1843, 2448, 2516, 3005
BsmI GAATGC 2 cut(s) 169, 2342
Bso31I GGTCTC 1 cut(s) 386
BsoBI CYCGRG 2 cut(s) 2431, 2663
Bsp119I TTCGAA 1 cut(s) 2730
Bsp120I GGGCCC 1 cut(s) 1105
Bsp1286I GDGCHC 4 cut(s) 1109, 1963, 2167, 2431
Bsp19I CCATGG 1 cut(s) 888
BspCNI CTCAG 5 cut(s) 871, 1015, 1445, 1916, 2164
BspFNI CGCG 3 cut(s) 649, 2034, 3023
BspHI TCATGA 3 cut(s) 324, 1464, 2377
BspMAI CTGCAG 3 cut(s) 246, 1386, 1392
BspPI GGATC 5 cut(s) 879, 892, 900, 934, 1683
BspT104I TTCGAA 1 cut(s) 2730
BspT107I GGYRCC 3 cut(s) 1144, 2164, 2864
BspTNI GGTCTC 1 cut(s) 386
BssNI GRCGYC 1 cut(s) 820
BssSI CACGAG 2 cut(s) 1202, 1333
BssT1I CCWWGG 6 cut(s) 376, 538, 888, 1109, 1721, 2494
Bst2BI CACGAG 2 cut(s) 1202, 1333
Bst2UI CCWGG 3 cut(s) 582, 2442, 2520
Bst4CI ACNGT 3 cut(s) 297, 1328, 2996
Bst6I CTCTTC 5 cut(s) 356, 799, 1496, 2237, 2355
BstACI GRCGYC 1 cut(s) 820
BstAPI GCANNNNNTGC 1 cut(s) 1043
BstBI TTCGAA 1 cut(s) 2730
BstC8I GCNNGC 8 cut(s) 303, 644, 757, 786, 1443, 2427, 2525, 2800
BstDEI CTNAG 9 cut(s) 519, 879, 1002, 1432, 1659, 1784, 1903, 2159, 2172
BstDSI CCRYGG 3 cut(s) 647, 888, 2536
BstEII GGTNACC 4 cut(s) 631, 686, 719, 2498
BstENI CCTNNNNNAGG 1 cut(s) 2768
BstFNI CGCG 3 cut(s) 649, 2034, 3023
BstH2I RGCGCY 1 cut(s) 791
BstHHI GCGC 2 cut(s) 790, 1953
BstMAI GTCTC 5 cut(s) 358, 386, 827, 1077, 1498
BstMCI CGRYCG 4 cut(s) 647, 684, 862, 1166
BstNI CCWGG 3 cut(s) 582, 2442, 2520
BstPI GGTNACC 4 cut(s) 631, 686, 719, 2498
BstSCI CCNGG 9 cut(s) 185, 417, 580, 1557, 2430, 2431, 2440, 2518, 2583
BstSFI CTRYAG 3 cut(s) 242, 1382, 1388
BstSLI GKGCMC 2 cut(s) 1109, 2167
BstUI CGCG 3 cut(s) 649, 2034, 3023
BstV2I GAAGAC 4 cut(s) 1918, 2079, 2702, 3012
BstX2I RGATCY 4 cut(s) 884, 892, 939, 1675
BstXI CCANNNNNNTGG 4 cut(s) 407, 1547, 1697, 1728
BstYI RGATCY 4 cut(s) 884, 892, 939, 1675
BstZI CGGCCG 1 cut(s) 644
BtgI CCRYGG 3 cut(s) 647, 888, 2536
BtrI CACGTC 1 cut(s) 2704
BtsI GCAGTG 3 cut(s) 221, 347, 1487
BtsIMutI CAGTG 8 cut(s) 221, 347, 439, 643, 1487, 1579, 1911, 3001
Cac8I GCNNGC 8 cut(s) 303, 644, 757, 786, 1443, 2427, 2525, 2800
CaiI CAGNNNCTG 1 cut(s) 1043
CciI TCATGA 3 cut(s) 324, 1464, 2377
CfoI GCGC 2 cut(s) 790, 1953
Cfr42I CCGCGG 1 cut(s) 650
Cfr9I CCCGGG 1 cut(s) 2431
CseI GACGC 3 cut(s) 434, 910, 2691
Csp6I GTAC 6 cut(s) 767, 2083, 2668, 2865, 3019, 3040
CspAI ACCGGT 3 cut(s) 662, 1028, 2065
CviQI GTAC 6 cut(s) 767, 2083, 2668, 2865, 3019, 3040
DdeI CTNAG 9 cut(s) 519, 879, 1002, 1432, 1659, 1784, 1903, 2159, 2172
DriI GACNNNNNGTC 1 cut(s) 3013
EaeI YGGCCR 6 cut(s) 468, 640, 644, 1608, 2453, 2539
EagI CGGCCG 1 cut(s) 644
Eam1104I CTCTTC 5 cut(s) 356, 799, 1496, 2237, 2355
Eam1105I GACNNNNNGTC 1 cut(s) 3013
EarI CTCTTC 5 cut(s) 356, 799, 1496, 2237, 2355
EciI GGCGGA 1 cut(s) 1086
Ecl136II GAGCTC 1 cut(s) 1961
EclXI CGGCCG 1 cut(s) 644
Eco130I CCWWGG 6 cut(s) 376, 538, 888, 1109, 1721, 2494
Eco147I AGGCCT 2 cut(s) 2493, 2523
Eco24I GRGCYC 3 cut(s) 1109, 1963, 2431
Eco31I GGTCTC 1 cut(s) 386
Eco32I GATATC 2 cut(s) 415, 1555
Eco47I GGWCC 9 cut(s) 189, 344, 535, 762, 1484, 1835, 1968, 2587, 2832
Eco52I CGGCCG 1 cut(s) 644
Eco53kI GAGCTC 1 cut(s) 1961
Eco57I CTGAAG 3 cut(s) 1260, 2567, 2912
Eco88I CYCGRG 2 cut(s) 2431, 2663
Eco91I GGTNACC 4 cut(s) 631, 686, 719, 2498
EcoICRI GAGCTC 1 cut(s) 1961
EcoNI CCTNNNNNAGG 1 cut(s) 2768
EcoO109I RGGNCCY 2 cut(s) 189, 1105
EcoO65I GGTNACC 4 cut(s) 631, 686, 719, 2498
EcoRI GAATTC 2 cut(s) 127, 1303
EcoRII CCWGG 3 cut(s) 580, 2440, 2518
EcoRV GATATC 2 cut(s) 415, 1555
EcoT14I CCWWGG 6 cut(s) 376, 538, 888, 1109, 1721, 2494
EcoT38I GRGCYC 3 cut(s) 1109, 1963, 2431
ErhI CCWWGG 6 cut(s) 376, 538, 888, 1109, 1721, 2494
Esp3I CGTCTC 2 cut(s) 827, 1077
FalI AAGNNNNNCTT 4 cut(s) 449, 481, 2142, 2174
FaqI GGGAC 6 cut(s) 175, 685, 1843, 2448, 2516, 3005
FauI CCCGC 4 cut(s) 1339, 1358, 2468, 2761
FbaI TGATCA 2 cut(s) 2287, 2469
FblI GTMKAC 1 cut(s) 1524
FriOI GRGCYC 3 cut(s) 1109, 1963, 2431
FseI GGCCGGCC 1 cut(s) 646
FspBI CTAG 9 cut(s) 87, 96, 152, 1149, 1218, 1292, 2406, 2495, 2591
GlaI GCGC 2 cut(s) 789, 1952
GsaI CCCAGC 1 cut(s) 2806
GsuI CTGGAG 4 cut(s) 269, 639, 809, 1136
HaeII RGCGCY 1 cut(s) 791
HgaI GACGC 3 cut(s) 434, 910, 2691
HhaI GCGC 2 cut(s) 790, 1953
Hin1I GRCGYC 1 cut(s) 820
Hin6I GCGC 2 cut(s) 788, 1951
HinP1I GCGC 2 cut(s) 788, 1951
HincII GTYRAC 2 cut(s) 511, 2809
HindII GTYRAC 2 cut(s) 511, 2809
HindIII AAGCTT 4 cut(s) 319, 458, 1459, 2398
Hpy99I CGWCG 5 cut(s) 822, 1968, 2705, 3019, 3049
HpyAV CCTTC 9 cut(s) 130, 992, 1235, 2301, 2854, 2873, 2930, 2977, 3085
HpyCH4III ACNGT 3 cut(s) 297, 1328, 2996
HpyCH4IV ACGT 4 cut(s) 820, 1733, 2559, 2703
HpyF3I CTNAG 9 cut(s) 519, 879, 1002, 1432, 1659, 1784, 1903, 2159, 2172
HpySE526I ACGT 4 cut(s) 820, 1733, 2559, 2703
Hsp92I GRCGYC 1 cut(s) 820
HspAI GCGC 2 cut(s) 788, 1951
KflI GGGWCCC 1 cut(s) 189
KpnI GGTACC 1 cut(s) 2868
KroI GCCGGC 4 cut(s) 301, 642, 784, 1441
KroNI GCCGGC 4 cut(s) 303, 644, 786, 1443
Ksp22I TGATCA 2 cut(s) 2287, 2469
KspI CCGCGG 1 cut(s) 650
LmnI GCTCC 8 cut(s) 228, 909, 935, 1000, 1368, 2190, 2334, 2709
LweI GCATC 6 cut(s) 728, 1555, 1786, 1787, 2727, 2877
MaeI CTAG 9 cut(s) 87, 96, 152, 1149, 1218, 1292, 2406, 2495, 2591
MaeII ACGT 4 cut(s) 820, 1733, 2559, 2703
MfeI CAATTG 1 cut(s) 35
MflI RGATCY 4 cut(s) 884, 892, 939, 1675
MhlI GDGCHC 4 cut(s) 1109, 1963, 2167, 2431
MlsI TGGCCA 3 cut(s) 470, 1610, 2455
MluCI AATT 9 cut(s) 35, 127, 279, 551, 1303, 1541, 1711, 1789, 2051
MluNI TGGCCA 3 cut(s) 470, 1610, 2455
MlyI GAGTC 5 cut(s) 646, 994, 1074, 1866, 2439
Mox20I TGGCCA 3 cut(s) 470, 1610, 2455
MreI CGCCGGCG 1 cut(s) 784
MroNI GCCGGC 4 cut(s) 301, 642, 784, 1441
MroXI GAANNNNTTC 1 cut(s) 1541
MscI TGGCCA 3 cut(s) 470, 1610, 2455
MseI TTAA 2 cut(s) 1739, 1943
MslI CAYNNNNRTG 2 cut(s) 1904, 2415
Msp20I TGGCCA 3 cut(s) 470, 1610, 2455
MspA1I CMGCKG 7 cut(s) 247, 649, 878, 927, 1387, 2171, 2802
MspR9I CCNGG 9 cut(s) 187, 419, 582, 1559, 2432, 2433, 2442, 2520, 2585
MunI CAATTG 1 cut(s) 35
Mva1269I GAATGC 2 cut(s) 169, 2342
MvaI CCWGG 3 cut(s) 582, 2442, 2520
MvnI CGCG 3 cut(s) 649, 2034, 3023
NaeI GCCGGC 4 cut(s) 303, 644, 786, 1443
NciI CCSGG 6 cut(s) 187, 419, 1559, 2432, 2433, 2585
NcoI CCATGG 1 cut(s) 888
NgoMIV GCCGGC 4 cut(s) 301, 642, 784, 1441
NmeAIII GCCGAG 3 cut(s) 125, 1265, 2694
NmuCI GTSAC 7 cut(s) 631, 665, 686, 719, 1244, 2313, 3055
NspV TTCGAA 1 cut(s) 2730
PagI TCATGA 3 cut(s) 324, 1464, 2377
PceI AGGCCT 2 cut(s) 2493, 2523
PcsI WCGNNNNNNNCGW 4 cut(s) 1368, 1969, 2565, 3020
PctI GAATGC 2 cut(s) 169, 2342
PdiI GCCGGC 4 cut(s) 303, 644, 786, 1443
PdmI GAANNNNTTC 1 cut(s) 1541
PfeI GAWTC 5 cut(s) 565, 694, 701, 1121, 1771
Pfl23II CGTACG 1 cut(s) 3018
PfoI TCCNGGA 2 cut(s) 417, 1557
PinAI ACCGGT 3 cut(s) 662, 1028, 2065
Ple19I CGATCG 3 cut(s) 684, 862, 1166
PleI GAGTC 5 cut(s) 646, 994, 1073, 1866, 2439
PpsI GAGTC 5 cut(s) 646, 994, 1073, 1866, 2439
PpuMI RGGWCCY 1 cut(s) 189
Psp124BI GAGCTC 1 cut(s) 1963
Psp5II RGGWCCY 1 cut(s) 189
Psp6I CCWGG 3 cut(s) 580, 2440, 2518
PspEI GGTNACC 4 cut(s) 631, 686, 719, 2498
PspFI CCCAGC 1 cut(s) 2802
PspGI CCWGG 3 cut(s) 580, 2440, 2518
PspLI CGTACG 1 cut(s) 3018
PspOMI GGGCCC 1 cut(s) 1105
PspPPI RGGWCCY 1 cut(s) 189
PstI CTGCAG 3 cut(s) 246, 1386, 1392
PstNI CAGNNNCTG 1 cut(s) 1043
PsuI RGATCY 4 cut(s) 884, 892, 939, 1675
PvuI CGATCG 3 cut(s) 684, 862, 1166
PvuII CAGCTG 6 cut(s) 247, 878, 927, 1387, 2171, 2802
RigI GGCCGGCC 1 cut(s) 646
RsaI GTAC 6 cut(s) 768, 2084, 2669, 2866, 3020, 3041
RsaNI GTAC 6 cut(s) 767, 2083, 2668, 2865, 3019, 3040
RseI CAYNNNNRTG 2 cut(s) 1904, 2415
SacI GAGCTC 1 cut(s) 1963
SacII CCGCGG 1 cut(s) 650
SaqAI TTAA 2 cut(s) 1739, 1943
ScaI AGTACT 1 cut(s) 2669
SchI GAGTC 5 cut(s) 646, 994, 1074, 1866, 2439
ScrFI CCNGG 9 cut(s) 187, 419, 582, 1559, 2432, 2433, 2442, 2520, 2585
SduI GDGCHC 4 cut(s) 1109, 1963, 2167, 2431
SfaNI GCATC 6 cut(s) 728, 1555, 1786, 1787, 2727, 2877
SfcI CTRYAG 3 cut(s) 242, 1382, 1388
SfiI GGCCNNNNNGGCC 2 cut(s) 311, 1407
Sfr303I CCGCGG 1 cut(s) 650
SfuI TTCGAA 1 cut(s) 2730
SgrAI CRCCGGYG 3 cut(s) 784, 963, 1028
SgrBI CCGCGG 1 cut(s) 650
SinI GGWCC 9 cut(s) 189, 344, 535, 762, 1484, 1835, 1968, 2587, 2832
SmaI CCCGGG 1 cut(s) 2433
SmiMI CAYNNNNRTG 2 cut(s) 1904, 2415
SmlI CTYRAG 2 cut(s) 308, 1448
SmoI CTYRAG 2 cut(s) 308, 1448
Sse9I AATT 9 cut(s) 35, 127, 279, 551, 1303, 1541, 1711, 1789, 2051
SseBI AGGCCT 2 cut(s) 2493, 2523
SspMI CTAG 9 cut(s) 87, 96, 152, 1149, 1218, 1292, 2406, 2495, 2591
SstI GAGCTC 1 cut(s) 1963
StuI AGGCCT 2 cut(s) 2493, 2523
StyD4I CCNGG 9 cut(s) 185, 417, 580, 1557, 2430, 2431, 2440, 2518, 2583
StyI CCWWGG 6 cut(s) 376, 538, 888, 1109, 1721, 2494
TaaI ACNGT 3 cut(s) 297, 1328, 2996
TaiI ACGT 4 cut(s) 823, 1736, 2562, 2706
TaqI TCGA 9 cut(s) 817, 1166, 1957, 2448, 2568, 2730, 2847, 2973, 3047
TaqII GACCGA 1 cut(s) 2714
TasI AATT 9 cut(s) 35, 127, 279, 551, 1303, 1541, 1711, 1789, 2051
TatI WGTACW 3 cut(s) 2082, 2667, 3039
TauI GCSGC 1 cut(s) 649
TfiI GAWTC 5 cut(s) 565, 694, 701, 1121, 1771
Tru1I TTAA 2 cut(s) 1739, 1943
Tru9I TTAA 2 cut(s) 1739, 1943
TscAI CASTG 8 cut(s) 221, 354, 439, 643, 1494, 1579, 1911, 3001
TseFI GTSAC 7 cut(s) 631, 665, 686, 719, 1244, 2313, 3055
Tsp45I GTSAC 7 cut(s) 631, 665, 686, 719, 1244, 2313, 3055
TspGWI ACGGA 3 cut(s) 754, 896, 2409
TspMI CCCGGG 1 cut(s) 2431
TspRI CASTG 8 cut(s) 221, 354, 439, 643, 1494, 1579, 1911, 3001
VpaK11BI GGWCC 9 cut(s) 189, 344, 535, 762, 1484, 1835, 1968, 2587, 2832
XagI CCTNNNNNAGG 1 cut(s) 2768
XapI RAATTY 3 cut(s) 127, 1303, 1711
XcmI CCANNNNNNNNNTGG 1 cut(s) 2448
XmaI CCCGGG 1 cut(s) 2431
XmaJI CCTAGG 1 cut(s) 2494
XmiI GTMKAC 1 cut(s) 1524
XmnI GAANNNNTTC 1 cut(s) 1541
XspI CTAG 9 cut(s) 87, 96, 152, 1149, 1218, 1292, 2406, 2495, 2591
ZraI GACGTC 1 cut(s) 821
ZrmI AGTACT 1 cut(s) 2669
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.