Rmu_ssc0000252.1_g000039

protein At2g39795, mitochondrial-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_ssc0000252.1
OG0008277
Physical Location & Seq
Forward (+)
236766 .. 237481
716 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 504 bp
atgtcgagcccaagaccctcaccgacgccgtccaccgtccaagactggccctggcccacccagccgcaacaacttcggatccgccgacgtcagcccctccggcctcctcgcattcccggccggcagctccatctccgtcatcgacacccgctcaatgaagctcgtcgtctccctccactgagccttccagctcccatctcctcgccgccggcgaccgccagggccgcatcgccctcctcgacctccgcctcaagggaggctgcagatgggtttccttttgaaatagtagacaatgccggagagcagaccataatgctcagaagagaatttgatggagaaatcatcagagtggctgtcgatattcctaatattcctaccgctgacgacaatgatgatgaggaggagaatgaagacactgggattccaatggttgtgagtgtttcaatggagaagagactgttgttgtctggagaagaaagagagaagaggaagagttgtcgttag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
ko05168 map05168
Pfam Domains
Protein Families
MAM33

Protein Analysis

167

Amino Acids

18.78

Weight (kDa)

6.85

Isoelectric Point (pI)

83.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MAM33 PF02330 80 - 138 2.6e-09 Mitochondrial glycoprotein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 91
AccBSI CCGCTC 1 cut(s) 151
AccI GTMKAC 1 cut(s) 288
AciI CCGC 8 cut(s) 65, 82, 149, 206, 216, 225, 246, 378
AclWI GGATC 2 cut(s) 73, 86
AcoI YGGCCR 1 cut(s) 118
AcsI RAATTY 1 cut(s) 326
AcyI GRCGYC 2 cut(s) 26, 88
AfiI CCNNNNNNNGG 1 cut(s) 252
AgsI TTSAA 2 cut(s) 281, 444
AjnI CCWGG 2 cut(s) 50, 218
AluBI AGCT 3 cut(s) 127, 161, 191
AluI AGCT 3 cut(s) 127, 161, 191
Alw26I GTCTC 2 cut(s) 173, 448
AlwI GGATC 2 cut(s) 73, 86
AoxI GGCC 5 cut(s) 47, 53, 101, 118, 222
ApeKI GCWGC 2 cut(s) 124, 260
ApoI RAATTY 1 cut(s) 326
AspS9I GGNCC 3 cut(s) 48, 54, 222
AsuC2I CCSGG 1 cut(s) 117
AsuHPI GGTGA 1 cut(s) 12
BamHI GGATCC 1 cut(s) 78
BanII GRGCYC 1 cut(s) 11
BbsI GAAGAC 1 cut(s) 417
BbvI GCAGC 2 cut(s) 136, 247
BccI CCATC 4 cut(s) 138, 203, 260, 326
BceAI ACGGC 1 cut(s) 13
BcgI CGANNNNNNTGC 2 cut(s) 56, 90
BciT130I CCWGG 2 cut(s) 52, 220
BcnI CCSGG 1 cut(s) 117
BcoDI GTCTC 2 cut(s) 173, 448
BfmI CTRYAG 1 cut(s) 261
BglI GCCNNNNNGGC 1 cut(s) 100
BisI GCNGC 5 cut(s) 65, 125, 206, 225, 261
BlsI GCNGC 5 cut(s) 66, 126, 207, 226, 262
Bme1390I CCNGG 3 cut(s) 52, 117, 220
BmgT120I GGNCC 3 cut(s) 48, 54, 222
BmiI GGNNCC 1 cut(s) 80
BmrFI CCNGG 3 cut(s) 52, 117, 220
BmrI ACTGGG 1 cut(s) 426
BmsI GCATC 1 cut(s) 236
BmuI ACTGGG 1 cut(s) 426
BpiI GAAGAC 1 cut(s) 417
BpmI CTGGAG 1 cut(s) 489
BpuEI CTTGAG 1 cut(s) 235
BpuMI CCSGG 1 cut(s) 117
BsaHI GRCGYC 2 cut(s) 26, 88
BsaJI CCNNGG 2 cut(s) 50, 219
Bsc4I CCNNNNNNNGG 1 cut(s) 252
Bse118I RCCGGY 2 cut(s) 120, 208
Bse1I ACTGG 2 cut(s) 50, 421
BseBI CCWGG 2 cut(s) 52, 220
BseDI CCNNGG 2 cut(s) 50, 219
BseLI CCNNNNNNNGG 1 cut(s) 252
BseMII CTCAG 2 cut(s) 170, 331
BseNI ACTGG 2 cut(s) 50, 421
BseRI GAGGAG 5 cut(s) 96, 190, 226, 413, 416
BseX3I CGGCCG 1 cut(s) 118
BseXI GCAGC 2 cut(s) 136, 247
BseYI CCCAGC 1 cut(s) 60
Bsh1285I CGRYCG 2 cut(s) 121, 216
BshFI GGCC 5 cut(s) 49, 55, 103, 120, 224
BsiEI CGRYCG 2 cut(s) 121, 216
BsiSI CCGG 5 cut(s) 100, 117, 121, 209, 297
BslI CCNNNNNNNGG 1 cut(s) 252
BsmAI GTCTC 2 cut(s) 173, 448
BsmBI CGTCTC 1 cut(s) 173
BsmI GAATGC 1 cut(s) 111
BsnI GGCC 5 cut(s) 49, 55, 103, 120, 224
Bsp1286I GDGCHC 1 cut(s) 11
Bsp143I GATC 1 cut(s) 78
BspACI CCGC 8 cut(s) 65, 82, 149, 206, 216, 225, 246, 378
BspANI GGCC 5 cut(s) 49, 55, 103, 120, 224
BspCNI CTCAG 2 cut(s) 171, 330
BspLI GGNNCC 1 cut(s) 80
BspMAI CTGCAG 1 cut(s) 265
BspPI GGATC 2 cut(s) 73, 86
BsrBI CCGCTC 1 cut(s) 151
BsrFI RCCGGY 2 cut(s) 120, 208
BsrI ACTGG 2 cut(s) 50, 421
BssAI RCCGGY 2 cut(s) 120, 208
BssECI CCNNGG 2 cut(s) 50, 219
BssMI GATC 1 cut(s) 78
BssNI GRCGYC 2 cut(s) 26, 88
Bst2UI CCWGG 2 cut(s) 52, 220
Bst4CI ACNGT 2 cut(s) 37, 459
Bst6I CTCTTC 4 cut(s) 316, 446, 479, 485
BstACI GRCGYC 2 cut(s) 26, 88
BstC8I GCNNGC 2 cut(s) 122, 210
BstDEI CTNAG 2 cut(s) 179, 317
BstKTI GATC 1 cut(s) 81
BstMAI GTCTC 2 cut(s) 173, 448
BstMBI GATC 1 cut(s) 78
BstMCI CGRYCG 2 cut(s) 121, 216
BstMWI GCNNNNNNNGC 4 cut(s) 61, 100, 117, 224
BstNI CCWGG 2 cut(s) 52, 220
BstSCI CCNGG 3 cut(s) 50, 115, 218
BstSFI CTRYAG 1 cut(s) 261
BstV1I GCAGC 2 cut(s) 136, 247
BstV2I GAAGAC 1 cut(s) 417
BstX2I RGATCY 1 cut(s) 78
BstYI RGATCY 1 cut(s) 78
BstZI CGGCCG 1 cut(s) 118
BsuRI GGCC 5 cut(s) 49, 55, 103, 120, 224
BtgZI GCGATG 1 cut(s) 213
BtsIMutI CAGTG 2 cut(s) 176, 414
Cac8I GCNNGC 2 cut(s) 122, 210
Cfr10I RCCGGY 2 cut(s) 120, 208
Cfr13I GGNCC 3 cut(s) 48, 54, 222
CseI GACGC 1 cut(s) 34
DdeI CTNAG 2 cut(s) 179, 317
DpnI GATC 1 cut(s) 80
DpnII GATC 1 cut(s) 78
EaeI YGGCCR 1 cut(s) 118
EagI CGGCCG 1 cut(s) 118
Eam1104I CTCTTC 4 cut(s) 316, 446, 479, 485
EarI CTCTTC 4 cut(s) 316, 446, 479, 485
EciI GGCGGA 2 cut(s) 71, 235
EclXI CGGCCG 1 cut(s) 118
Eco24I GRGCYC 1 cut(s) 11
Eco52I CGGCCG 1 cut(s) 118
EcoRII CCWGG 2 cut(s) 50, 218
EcoT38I GRGCYC 1 cut(s) 11
Esp3I CGTCTC 1 cut(s) 173
FaiI YATR 1 cut(s) 311
FauI CCCGC 1 cut(s) 156
FblI GTMKAC 1 cut(s) 288
Fnu4HI GCNGC 5 cut(s) 65, 125, 206, 225, 261
FriOI GRGCYC 1 cut(s) 11
Fsp4HI GCNGC 5 cut(s) 65, 125, 206, 225, 261
GluI GCNGC 5 cut(s) 65, 125, 206, 225, 261
GsaI CCCAGC 1 cut(s) 64
GsuI CTGGAG 1 cut(s) 489
HaeIII GGCC 5 cut(s) 49, 55, 103, 120, 224
HapII CCGG 5 cut(s) 100, 117, 121, 209, 297
HgaI GACGC 1 cut(s) 34
Hin1I GRCGYC 2 cut(s) 26, 88
HinfI GANTC 1 cut(s) 421
HpaII CCGG 5 cut(s) 100, 117, 121, 209, 297
HphI GGTGA 1 cut(s) 12
Hpy166II GTNNAC 2 cut(s) 33, 289
Hpy188I TCNGA 3 cut(s) 78, 320, 347
Hpy188III TCNNGA 1 cut(s) 468
Hpy8I GTNNAC 2 cut(s) 33, 289
Hpy99I CGWCG 3 cut(s) 28, 90, 168
HpyAV CCTTC 1 cut(s) 194
HpyCH4III ACNGT 2 cut(s) 37, 459
HpyCH4IV ACGT 1 cut(s) 88
HpyCH4V TGCA 1 cut(s) 263
HpyF10VI GCNNNNNNNGC 4 cut(s) 61, 100, 117, 224
HpyF3I CTNAG 2 cut(s) 179, 317
HpySE526I ACGT 1 cut(s) 88
Hsp92I GRCGYC 2 cut(s) 26, 88
KroI GCCGGC 2 cut(s) 120, 208
KroNI GCCGGC 2 cut(s) 122, 210
Kzo9I GATC 1 cut(s) 78
LmnI GCTCC 2 cut(s) 132, 196
Lsp1109I GCAGC 2 cut(s) 136, 247
LweI GCATC 1 cut(s) 236
MaeII ACGT 1 cut(s) 88
MalI GATC 1 cut(s) 80
MbiI CCGCTC 1 cut(s) 151
MboI GATC 1 cut(s) 78
MboII GAAGA 6 cut(s) 333, 422, 463, 485, 496, 502
MflI RGATCY 1 cut(s) 78
MhlI GDGCHC 1 cut(s) 11
MluCI AATT 1 cut(s) 326
MreI CGCCGGCG 1 cut(s) 208
MroNI GCCGGC 2 cut(s) 120, 208
MslI CAYNNNNRTG 1 cut(s) 347
MspA1I CMGCKG 1 cut(s) 380
MspI CCGG 5 cut(s) 100, 117, 121, 209, 297
MspR9I CCNGG 3 cut(s) 52, 117, 220
Mva1269I GAATGC 1 cut(s) 111
MvaI CCWGG 2 cut(s) 52, 220
MwoI GCNNNNNNNGC 4 cut(s) 61, 100, 117, 224
NaeI GCCGGC 2 cut(s) 122, 210
NciI CCSGG 1 cut(s) 117
NdeII GATC 1 cut(s) 78
NgoMIV GCCGGC 2 cut(s) 120, 208
NlaIV GGNNCC 1 cut(s) 80
PcsI WCGNNNNNNNCGW 3 cut(s) 82, 209, 236
PctI GAATGC 1 cut(s) 111
PdiI GCCGGC 2 cut(s) 122, 210
PfeI GAWTC 1 cut(s) 421
PflFI GACNNNGTC 1 cut(s) 28
PkrI GCNGC 5 cut(s) 66, 126, 207, 226, 262
Psp6I CCWGG 2 cut(s) 50, 218
PspFI CCCAGC 1 cut(s) 60
PspGI CCWGG 2 cut(s) 50, 218
PspN4I GGNNCC 1 cut(s) 80
PspPI GGNCC 3 cut(s) 48, 54, 222
PstI CTGCAG 1 cut(s) 265
PsuI RGATCY 1 cut(s) 78
PsyI GACNNNGTC 1 cut(s) 28
RseI CAYNNNNRTG 1 cut(s) 347
SatI GCNGC 5 cut(s) 65, 125, 206, 225, 261
Sau3AI GATC 1 cut(s) 78
Sau96I GGNCC 3 cut(s) 48, 54, 222
ScrFI CCNGG 3 cut(s) 52, 117, 220
SduI GDGCHC 1 cut(s) 11
SetI ASST 5 cut(s) 91, 129, 163, 193, 245
SfaNI GCATC 1 cut(s) 236
SfcI CTRYAG 1 cut(s) 261
SgrAI CRCCGGYG 1 cut(s) 208
SmiMI CAYNNNNRTG 1 cut(s) 347
SmlI CTYRAG 1 cut(s) 250
SmoI CTYRAG 1 cut(s) 250
Sse9I AATT 1 cut(s) 326
SsiI CCGC 8 cut(s) 65, 82, 149, 206, 216, 225, 246, 378
SspI AATATT 1 cut(s) 370
StyD4I CCNGG 3 cut(s) 50, 115, 218
TaaI ACNGT 2 cut(s) 37, 459
TaiI ACGT 1 cut(s) 91
TaqI TCGA 4 cut(s) 5, 142, 239, 357
TasI AATT 1 cut(s) 326
TauI GCSGC 3 cut(s) 67, 208, 227
TfiI GAWTC 1 cut(s) 421
TscAI CASTG 2 cut(s) 183, 421
TseI GCWGC 2 cut(s) 124, 260
TspDTI ATGAA 2 cut(s) 171, 423
TspGWI ACGGA 1 cut(s) 125
TspRI CASTG 2 cut(s) 183, 421
Tth111I GACNNNGTC 1 cut(s) 28
XapI RAATTY 1 cut(s) 326
XmiI GTMKAC 1 cut(s) 288
ZraI GACGTC 1 cut(s) 89
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.