Rmu_ssc0000397.1_g000002

Indigoidine synthase A like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_ssc0000397.1
OG0007924
Physical Location & Seq
Reverse (-)
19516 .. 25490
5975 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 735 bp
atgtctgagatggcagagctaaaattctcacttccagcacctctagcattccaaaaaagaattttaaacattaaaaaagtgggaaggagagggtacacaccccagcttagacatcaaccttaccctccccatcatgaggaggggggcagaccaaaaatagctgaaattcctttgtctaagacatcagaacctatgaagcacggatacgccattttggcttcgtatcccgtaccgatccgggatacggctgtcatctctgctggtgtaaaatcaattttggatatcccaaggaccctcgaatatttggaaacacagggagtttgtgtagctgcttacaagaccaatgaatttccagcatttttcacagaagcaagtggctgcaaggtgccttgccgtatagataccccagatgattgcgctcggcttatagatgcaaaccttaaacttgagcttggaactggaatgttgattgctgttccaattcctaaagaacactcggcttctggaaatttaattgagtctgcaatacagactgctctcagagaagctagggataagaacataacaggcaatgctgctactccattcttgcttgcaagagtaaatgaactaactggaggagcatctttggcttcgaacattgccctggtgaagaataatgcacttgttggttctaaaattgctgtagcactttcccggatcagagaatgttgtagaaaaggtggtttggagtaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00240 map00240
Pfam Domains
Protein Families
Indigoidine_A

Protein Analysis

244

Amino Acids

26.38

Weight (kDa)

9.12

Isoelectric Point (pI)

38.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Indigoidine_A PF04227 81 - 230 3.2e-58 Indigoidine synthase A like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 385
AclWI GGATC 2 cut(s) 229, 707
AcsI RAATTY 5 cut(s) 23, 60, 165, 347, 508
AfaI GTAC 2 cut(s) 95, 231
AfiI CCNNNNNNNGG 2 cut(s) 136, 244
AjnI CCWGG 1 cut(s) 645
AluBI AGCT 6 cut(s) 19, 106, 161, 329, 451, 548
AluI AGCT 6 cut(s) 19, 106, 161, 329, 451, 548
AlwI GGATC 2 cut(s) 229, 707
ApeKI GCWGC 3 cut(s) 329, 378, 575
ApoI RAATTY 5 cut(s) 23, 60, 165, 347, 508
AspLEI GCGC 1 cut(s) 419
AspS9I GGNCC 1 cut(s) 291
AsuC2I CCSGG 2 cut(s) 239, 697
AsuHPI GGTGA 1 cut(s) 661
AsuII TTCGAA 1 cut(s) 635
AvaII GGWCC 1 cut(s) 291
BaeI ACNNNNGTAYC 2 cut(s) 234, 267
BanI GGYRCC 1 cut(s) 385
BarI GAAGNNNNNNTAC 2 cut(s) 188, 220
BbvI GCAGC 3 cut(s) 316, 365, 562
BccI CCATC 2 cut(s) 4, 138
BceAI ACGGC 2 cut(s) 261, 378
BciT130I CCWGG 1 cut(s) 647
BciVI GTATCC 3 cut(s) 197, 234, 235
BcnI CCSGG 2 cut(s) 239, 697
BfaI CTAG 2 cut(s) 44, 549
BfmI CTRYAG 1 cut(s) 684
BfuI GTATCC 3 cut(s) 197, 234, 235
BglI GCCNNNNNGGC 1 cut(s) 215
BisI GCNGC 3 cut(s) 330, 379, 576
BlsI GCNGC 3 cut(s) 331, 380, 577
Bme1390I CCNGG 3 cut(s) 239, 647, 697
Bme18I GGWCC 1 cut(s) 291
BmgT120I GGNCC 1 cut(s) 291
BmiI GGNNCC 2 cut(s) 293, 387
BmrFI CCNGG 3 cut(s) 239, 647, 697
BmsI GCATC 2 cut(s) 421, 632
BpmI CTGGAG 1 cut(s) 636
Bpu14I TTCGAA 1 cut(s) 635
BpuEI CTTGAG 1 cut(s) 467
BpuMI CCSGG 2 cut(s) 239, 697
BsaJI CCNNGG 2 cut(s) 287, 645
BsaXI ACNNNNNCTCC 2 cut(s) 309, 339
Bsc4I CCNNNNNNNGG 2 cut(s) 136, 244
Bse1I ACTGG 2 cut(s) 463, 619
Bse3DI GCAATG 2 cut(s) 577, 639
BseBI CCWGG 1 cut(s) 647
BseDI CCNNGG 2 cut(s) 287, 645
BseLI CCNNNNNNNGG 2 cut(s) 136, 244
BseMI GCAATG 2 cut(s) 577, 639
BseMII CTCAG 1 cut(s) 553
BseNI ACTGG 2 cut(s) 463, 619
BseRI GAGGAG 2 cut(s) 152, 633
BseXI GCAGC 3 cut(s) 316, 365, 562
BseYI CCCAGC 1 cut(s) 102
BshNI GGYRCC 1 cut(s) 385
BsiSI CCGG 2 cut(s) 238, 697
BslI CCNNNNNNNGG 2 cut(s) 136, 244
BsmI GAATGC 1 cut(s) 47
Bsp119I TTCGAA 1 cut(s) 635
Bsp143I GATC 2 cut(s) 234, 699
BspCNI CTCAG 1 cut(s) 552
BspHI TCATGA 1 cut(s) 133
BspLI GGNNCC 2 cut(s) 293, 387
BspPI GGATC 2 cut(s) 229, 707
BspT104I TTCGAA 1 cut(s) 635
BspT107I GGYRCC 1 cut(s) 385
BsrDI GCAATG 2 cut(s) 577, 639
BsrI ACTGG 2 cut(s) 463, 619
BssECI CCNNGG 2 cut(s) 287, 645
BssMI GATC 2 cut(s) 234, 699
BssT1I CCWWGG 1 cut(s) 287
Bst2UI CCWGG 1 cut(s) 647
BstBI TTCGAA 1 cut(s) 635
BstC8I GCNNGC 1 cut(s) 594
BstDEI CTNAG 4 cut(s) 6, 107, 177, 539
BstHHI GCGC 1 cut(s) 419
BstKTI GATC 2 cut(s) 237, 702
BstMBI GATC 2 cut(s) 234, 699
BstMWI GCNNNNNNNGC 3 cut(s) 44, 215, 629
BstNI CCWGG 1 cut(s) 647
BstSCI CCNGG 3 cut(s) 237, 645, 695
BstSFI CTRYAG 1 cut(s) 684
BstV1I GCAGC 3 cut(s) 316, 365, 562
BsuI GTATCC 3 cut(s) 197, 234, 235
Cac8I GCNNGC 1 cut(s) 594
CciI TCATGA 1 cut(s) 133
CfoI GCGC 1 cut(s) 419
Cfr13I GGNCC 1 cut(s) 291
Csp6I GTAC 2 cut(s) 94, 230
CviAII CATG 1 cut(s) 134
CviQI GTAC 2 cut(s) 94, 230
DdeI CTNAG 4 cut(s) 6, 107, 177, 539
DpnI GATC 2 cut(s) 236, 701
DpnII GATC 2 cut(s) 234, 699
DraI TTTAAA 1 cut(s) 66
Eco130I CCWWGG 1 cut(s) 287
Eco32I GATATC 1 cut(s) 283
Eco47I GGWCC 1 cut(s) 291
EcoO109I RGGNCCY 1 cut(s) 291
EcoRII CCWGG 1 cut(s) 645
EcoRV GATATC 1 cut(s) 283
EcoT14I CCWWGG 1 cut(s) 287
ErhI CCWWGG 1 cut(s) 287
FaeI CATG 1 cut(s) 137
FaiI YATR 5 cut(s) 135, 194, 398, 428, 563
FatI CATG 1 cut(s) 133
Fnu4HI GCNGC 3 cut(s) 330, 379, 576
Fsp4HI GCNGC 3 cut(s) 330, 379, 576
FspBI CTAG 2 cut(s) 44, 549
GlaI GCGC 1 cut(s) 418
GluI GCNGC 3 cut(s) 330, 379, 576
GsaI CCCAGC 1 cut(s) 106
GsuI CTGGAG 1 cut(s) 636
HapII CCGG 2 cut(s) 238, 697
HhaI GCGC 1 cut(s) 419
Hin1II CATG 1 cut(s) 137
Hin6I GCGC 1 cut(s) 417
HinP1I GCGC 1 cut(s) 417
HinfI GANTC 1 cut(s) 518
HpaII CCGG 2 cut(s) 238, 697
HphI GGTGA 1 cut(s) 661
Hpy166II GTNNAC 1 cut(s) 96
Hpy188I TCNGA 4 cut(s) 7, 187, 542, 704
Hpy188III TCNNGA 2 cut(s) 134, 504
Hpy8I GTNNAC 1 cut(s) 96
HpyAV CCTTC 1 cut(s) 78
HpyCH4V TGCA 5 cut(s) 381, 434, 524, 596, 662
HpyF10VI GCNNNNNNNGC 3 cut(s) 44, 215, 629
HpyF3I CTNAG 4 cut(s) 6, 107, 177, 539
Hsp92II CATG 1 cut(s) 137
HspAI GCGC 1 cut(s) 417
Kzo9I GATC 2 cut(s) 234, 699
LmnI GCTCC 1 cut(s) 620
Lsp1109I GCAGC 3 cut(s) 316, 365, 562
LweI GCATC 2 cut(s) 421, 632
MaeI CTAG 2 cut(s) 44, 549
MalI GATC 2 cut(s) 236, 701
MboI GATC 2 cut(s) 234, 699
MboII GAAGA 1 cut(s) 664
MluCI AATT 9 cut(s) 23, 60, 165, 273, 347, 480, 508, 513, 678
MlyI GAGTC 1 cut(s) 527
MnlI CCTC 7 cut(s) 51, 83, 130, 133, 135, 305, 611
MseI TTAA 4 cut(s) 65, 72, 441, 512
MspI CCGG 2 cut(s) 238, 697
MspR9I CCNGG 3 cut(s) 239, 647, 697
Mva1269I GAATGC 1 cut(s) 47
MvaI CCWGG 1 cut(s) 647
MwoI GCNNNNNNNGC 3 cut(s) 44, 215, 629
NciI CCSGG 2 cut(s) 239, 697
NdeII GATC 2 cut(s) 234, 699
NlaIII CATG 1 cut(s) 137
NlaIV GGNNCC 2 cut(s) 293, 387
NmeAIII GCCGAG 2 cut(s) 400, 476
NspV TTCGAA 1 cut(s) 635
PagI TCATGA 1 cut(s) 133
PctI GAATGC 1 cut(s) 47
PfoI TCCNGGA 2 cut(s) 237, 695
PkrI GCNGC 3 cut(s) 331, 380, 577
PleI GAGTC 1 cut(s) 526
PpsI GAGTC 1 cut(s) 526
PpuMI RGGWCCY 1 cut(s) 291
Psp5II RGGWCCY 1 cut(s) 291
Psp6I CCWGG 1 cut(s) 645
PspFI CCCAGC 1 cut(s) 102
PspGI CCWGG 1 cut(s) 645
PspN4I GGNNCC 2 cut(s) 293, 387
PspPI GGNCC 1 cut(s) 291
PspPPI RGGWCCY 1 cut(s) 291
RsaI GTAC 2 cut(s) 95, 231
RsaNI GTAC 2 cut(s) 94, 230
SaqAI TTAA 4 cut(s) 65, 72, 441, 512
SatI GCNGC 3 cut(s) 330, 379, 576
Sau3AI GATC 2 cut(s) 234, 699
Sau96I GGNCC 1 cut(s) 291
SchI GAGTC 1 cut(s) 527
ScrFI CCNGG 3 cut(s) 239, 647, 697
SfaNI GCATC 2 cut(s) 421, 632
SfcI CTRYAG 1 cut(s) 684
SfuI TTCGAA 1 cut(s) 635
SinI GGWCC 1 cut(s) 291
SmlI CTYRAG 1 cut(s) 446
SmoI CTYRAG 1 cut(s) 446
Sse9I AATT 9 cut(s) 23, 60, 165, 273, 347, 480, 508, 513, 678
SspI AATATT 1 cut(s) 302
SspMI CTAG 2 cut(s) 44, 549
StyD4I CCNGG 3 cut(s) 237, 645, 695
StyI CCWWGG 1 cut(s) 287
TaqI TCGA 2 cut(s) 297, 635
TasI AATT 9 cut(s) 23, 60, 165, 273, 347, 480, 508, 513, 678
Tru1I TTAA 4 cut(s) 65, 72, 441, 512
Tru9I TTAA 4 cut(s) 65, 72, 441, 512
TseI GCWGC 3 cut(s) 329, 378, 575
TspDTI ATGAA 3 cut(s) 209, 360, 621
TspGWI ACGGA 1 cut(s) 216
VpaK11BI GGWCC 1 cut(s) 291
XapI RAATTY 5 cut(s) 23, 60, 165, 347, 508
XspI CTAG 2 cut(s) 44, 549
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.