Rmu_ssc0000404.1_g000009

The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_ssc0000404.1
OG0007091
Physical Location & Seq
Forward (+)
62322 .. 63178
857 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 303 bp
atgatctgcacttcttccttaaagccaaagctccgactgctccataaccgacgatggagatcgatcaaagctccgaagtttagaggacactcattggctgatcccgatgagctccgtgaccctgctgagaaggcacactatgctgccagagaccccatcacagccttgaagaaatacatattcgagcacaacttagccagtgaacacgagcagaaggccatagataagaagatagatgaggtgccaactcgccgctcccaactcgccgccagctgctcgagaatgtgtttgccgatcctatag

Protein Analysis

100

Amino Acids

11.53

Weight (kDa)

9.76

Isoelectric Point (pI)

68.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
E1_dh PF00676 27 - 80 7.7e-06 Dehydrogenase E1 component
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 241
AccBSI CCGCTC 1 cut(s) 255
AciI CCGC 2 cut(s) 253, 267
AclWI GGATC 2 cut(s) 95, 289
AgsI TTSAA 1 cut(s) 169
AluBI AGCT 4 cut(s) 31, 71, 112, 273
AluI AGCT 4 cut(s) 31, 71, 112, 273
Alw21I GWGCWC 2 cut(s) 114, 189
Alw26I GTCTC 1 cut(s) 144
AlwI GGATC 2 cut(s) 95, 289
Ama87I CYCGRG 1 cut(s) 277
AoxI GGCC 1 cut(s) 216
ApeKI GCWGC 2 cut(s) 143, 273
AvaI CYCGRG 1 cut(s) 277
BanI GGYRCC 1 cut(s) 241
BanII GRGCYC 1 cut(s) 114
BauI CACGAG 1 cut(s) 206
Bbv12I GWGCWC 2 cut(s) 114, 189
BbvI GCAGC 2 cut(s) 130, 260
BccI CCATC 2 cut(s) 48, 164
BcoDI GTCTC 1 cut(s) 144
BfmI CTRYAG 1 cut(s) 299
BisI GCNGC 4 cut(s) 144, 253, 267, 274
BlsI GCNGC 4 cut(s) 145, 254, 268, 275
BmeT110I CYCGRG 1 cut(s) 277
BmiI GGNNCC 1 cut(s) 243
Bsa29I ATCGAT 1 cut(s) 62
BsaBI GATNNNNATC 1 cut(s) 58
BsaI GGTCTC 1 cut(s) 144
Bse1I ACTGG 1 cut(s) 198
Bse8I GATNNNNATC 1 cut(s) 58
BseCI ATCGAT 1 cut(s) 62
BseJI GATNNNNATC 1 cut(s) 58
BseMII CTCAG 1 cut(s) 117
BseNI ACTGG 1 cut(s) 198
BseXI GCAGC 2 cut(s) 130, 260
BshFI GGCC 1 cut(s) 218
BshNI GGYRCC 1 cut(s) 241
BshVI ATCGAT 1 cut(s) 62
BsiHKAI GWGCWC 2 cut(s) 114, 189
BsiHKCI CYCGRG 1 cut(s) 277
BsmAI GTCTC 1 cut(s) 144
BsnI GGCC 1 cut(s) 218
Bso31I GGTCTC 1 cut(s) 144
BsoBI CYCGRG 1 cut(s) 277
Bsp1286I GDGCHC 2 cut(s) 114, 189
Bsp143I GATC 5 cut(s) 3, 59, 63, 100, 294
BspACI CCGC 2 cut(s) 253, 267
BspANI GGCC 1 cut(s) 218
BspCNI CTCAG 1 cut(s) 118
BspDI ATCGAT 1 cut(s) 62
BspLI GGNNCC 1 cut(s) 243
BspPI GGATC 2 cut(s) 95, 289
BspT107I GGYRCC 1 cut(s) 241
BspTNI GGTCTC 1 cut(s) 144
BsrBI CCGCTC 1 cut(s) 255
BsrI ACTGG 1 cut(s) 198
BssMI GATC 5 cut(s) 3, 59, 63, 100, 294
BssSI CACGAG 1 cut(s) 206
Bst2BI CACGAG 1 cut(s) 206
BstAPI GCANNNNNTGC 1 cut(s) 140
BstC8I GCNNGC 1 cut(s) 271
BstDEI CTNAG 2 cut(s) 126, 193
BstKTI GATC 5 cut(s) 6, 62, 66, 103, 297
BstMAI GTCTC 1 cut(s) 144
BstMBI GATC 5 cut(s) 3, 59, 63, 100, 294
BstMWI GCNNNNNNNGC 3 cut(s) 37, 131, 140
BstSFI CTRYAG 1 cut(s) 299
BstV1I GCAGC 2 cut(s) 130, 260
Bsu15I ATCGAT 1 cut(s) 62
BsuRI GGCC 1 cut(s) 218
BsuTUI ATCGAT 1 cut(s) 62
BtsIMutI CAGTG 1 cut(s) 205
Cac8I GCNNGC 1 cut(s) 271
ClaI ATCGAT 1 cut(s) 62
CviJI RGCY 9 cut(s) 25, 31, 71, 98, 112, 164, 197, 218, 273
CviKI_1 RGCY 9 cut(s) 25, 31, 71, 98, 112, 164, 197, 218, 273
DdeI CTNAG 2 cut(s) 126, 193
DpnI GATC 5 cut(s) 5, 61, 65, 102, 296
DpnII GATC 5 cut(s) 3, 59, 63, 100, 294
Ecl136II GAGCTC 1 cut(s) 112
Eco24I GRGCYC 1 cut(s) 114
Eco31I GGTCTC 1 cut(s) 144
Eco53kI GAGCTC 1 cut(s) 112
Eco88I CYCGRG 1 cut(s) 277
EcoICRI GAGCTC 1 cut(s) 112
EcoT38I GRGCYC 1 cut(s) 114
FaiI YATR 5 cut(s) 45, 141, 179, 221, 301
Fnu4HI GCNGC 4 cut(s) 144, 253, 267, 274
FriOI GRGCYC 1 cut(s) 114
Fsp4HI GCNGC 4 cut(s) 144, 253, 267, 274
GluI GCNGC 4 cut(s) 144, 253, 267, 274
HaeIII GGCC 1 cut(s) 218
Hpy166II GTNNAC 1 cut(s) 203
Hpy188I TCNGA 2 cut(s) 35, 75
Hpy188III TCNNGA 2 cut(s) 104, 279
Hpy8I GTNNAC 1 cut(s) 203
Hpy99I CGWCG 1 cut(s) 54
HpyAV CCTTC 2 cut(s) 124, 208
HpyCH4V TGCA 1 cut(s) 9
HpyF10VI GCNNNNNNNGC 3 cut(s) 37, 131, 140
HpyF3I CTNAG 2 cut(s) 126, 193
Kzo9I GATC 5 cut(s) 3, 59, 63, 100, 294
LmnI GCTCC 5 cut(s) 36, 45, 76, 117, 260
LpnPI CCDG 4 cut(s) 135, 160, 211, 283
Lsp1109I GCAGC 2 cut(s) 130, 260
MaeIII GTNAC 1 cut(s) 116
MalI GATC 5 cut(s) 5, 61, 65, 102, 296
MbiI CCGCTC 1 cut(s) 255
MboI GATC 5 cut(s) 3, 59, 63, 100, 294
MboII GAAGA 3 cut(s) 6, 181, 241
MhlI GDGCHC 2 cut(s) 114, 189
MmeI TCCRAC 1 cut(s) 58
MnlI CCTC 2 cut(s) 77, 232
MseI TTAA 1 cut(s) 20
MspA1I CMGCKG 1 cut(s) 273
MwoI GCNNNNNNNGC 3 cut(s) 37, 131, 140
NdeII GATC 5 cut(s) 3, 59, 63, 100, 294
NlaIV GGNNCC 1 cut(s) 243
NmuCI GTSAC 1 cut(s) 116
PaeR7I CTCGAG 1 cut(s) 277
PkrI GCNGC 4 cut(s) 145, 254, 268, 275
Psp124BI GAGCTC 1 cut(s) 114
PspN4I GGNNCC 1 cut(s) 243
PvuII CAGCTG 1 cut(s) 273
SacI GAGCTC 1 cut(s) 114
SaqAI TTAA 1 cut(s) 20
SatI GCNGC 4 cut(s) 144, 253, 267, 274
Sau3AI GATC 5 cut(s) 3, 59, 63, 100, 294
SduI GDGCHC 2 cut(s) 114, 189
SetI ASST 5 cut(s) 33, 73, 114, 243, 275
SfcI CTRYAG 1 cut(s) 299
Sfr274I CTCGAG 1 cut(s) 277
SlaI CTCGAG 1 cut(s) 277
SmlI CTYRAG 1 cut(s) 277
SmoI CTYRAG 1 cut(s) 277
SsiI CCGC 2 cut(s) 253, 267
SstI GAGCTC 1 cut(s) 114
TaqI TCGA 3 cut(s) 62, 183, 278
TauI GCSGC 2 cut(s) 255, 269
Tru1I TTAA 1 cut(s) 20
Tru9I TTAA 1 cut(s) 20
TscAI CASTG 1 cut(s) 205
TseFI GTSAC 1 cut(s) 116
TseI GCWGC 2 cut(s) 143, 273
Tsp45I GTSAC 1 cut(s) 116
TspGWI ACGGA 1 cut(s) 104
TspRI CASTG 1 cut(s) 205
XhoI CTCGAG 1 cut(s) 277
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.