Rw1G011520

The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0007091
Physical Location & Seq
Reverse (-)
25869254 .. 25870317
1064 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G011520.1

Sequence Viewer

Length: 399 bp
ATGGAGATCGATCAAAGCTCCGAAGTGCTGTTTAGAGGACACTCATTGGCTGATCCCGATGAGCTCCGTGACCCTGCTGAGAAGGCACACTATGCTGCCAGAGACCCCATCACAGCCTTGAAGAAATACATATTCGAGCACAACTTAGCCAGTGAACACGAGCAGAAGGCCATGGATAAGAAGATAGATGAGGTGCTTGAGGATGCCGTTGAGTTCGCCTATGAGAGCCCGCTCCCAACTCGCCGCCAGCTGCTCGAGAATGTGTTTGCCGATCCTATAGGCTTTGGAATAGGACCTGATGGCCGCTACAGATGTGAGGACCCTTTACTCAGGGCACTGCCGAAGTCTAGTCTTTGTTGGACCTATAGCAATCAACTTGTGCTATATCTCTGTTCCTAG

Protein Analysis

132

Amino Acids

15.07

Weight (kDa)

4.85

Isoelectric Point (pI)

39.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
E1_dh PF00676 12 - 79 1.2e-08 Dehydrogenase E1 component
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 232
AciI CCGC 3 cut(s) 230, 244, 304
AclWI GGATC 2 cut(s) 47, 266
AcoI YGGCCR 1 cut(s) 301
AgsI TTSAA 1 cut(s) 121
AluBI AGCT 3 cut(s) 18, 64, 250
AluI AGCT 3 cut(s) 18, 64, 250
Alw21I GWGCWC 2 cut(s) 66, 141
Alw26I GTCTC 1 cut(s) 96
AlwI GGATC 2 cut(s) 47, 266
Ama87I CYCGRG 1 cut(s) 254
AoxI GGCC 2 cut(s) 168, 301
ApeKI GCWGC 2 cut(s) 95, 250
AspS9I GGNCC 3 cut(s) 293, 319, 360
AvaI CYCGRG 1 cut(s) 254
AvaII GGWCC 3 cut(s) 293, 319, 360
BaeGI GKGCMC 1 cut(s) 337
BanII GRGCYC 2 cut(s) 66, 230
BauI CACGAG 1 cut(s) 158
Bbv12I GWGCWC 2 cut(s) 66, 141
BbvI GCAGC 2 cut(s) 82, 237
BccI CCATC 2 cut(s) 116, 293
BceAI ACGGC 1 cut(s) 191
BcoDI GTCTC 1 cut(s) 96
BfaI CTAG 2 cut(s) 348, 397
BfmI CTRYAG 3 cut(s) 276, 307, 364
BisI GCNGC 4 cut(s) 96, 244, 251, 304
BlsI GCNGC 4 cut(s) 97, 245, 252, 305
Bme18I GGWCC 3 cut(s) 293, 319, 360
BmeT110I CYCGRG 1 cut(s) 254
BmgT120I GGNCC 3 cut(s) 293, 319, 360
BmiI GGNNCC 1 cut(s) 321
BmsI GCATC 1 cut(s) 193
BpuEI CTTGAG 1 cut(s) 218
Bsa29I ATCGAT 1 cut(s) 9
BsaI GGTCTC 1 cut(s) 96
BsaJI CCNNGG 1 cut(s) 171
Bse1I ACTGG 1 cut(s) 150
BseCI ATCGAT 1 cut(s) 9
BseDI CCNNGG 1 cut(s) 171
BseGI GGATG 1 cut(s) 208
BseMII CTCAG 2 cut(s) 69, 343
BseNI ACTGG 1 cut(s) 150
BseSI GKGCMC 1 cut(s) 337
BseXI GCAGC 2 cut(s) 82, 237
BshFI GGCC 2 cut(s) 170, 303
BshVI ATCGAT 1 cut(s) 9
BsiHKAI GWGCWC 2 cut(s) 66, 141
BsiHKCI CYCGRG 1 cut(s) 254
BsmAI GTCTC 1 cut(s) 96
BsnI GGCC 2 cut(s) 170, 303
Bso31I GGTCTC 1 cut(s) 96
BsoBI CYCGRG 1 cut(s) 254
Bsp1286I GDGCHC 4 cut(s) 66, 141, 230, 337
Bsp143I GATC 4 cut(s) 6, 10, 52, 271
Bsp19I CCATGG 1 cut(s) 171
BspACI CCGC 3 cut(s) 230, 244, 304
BspANI GGCC 2 cut(s) 170, 303
BspCNI CTCAG 2 cut(s) 70, 342
BspDI ATCGAT 1 cut(s) 9
BspLI GGNNCC 1 cut(s) 321
BspPI GGATC 2 cut(s) 47, 266
BspTNI GGTCTC 1 cut(s) 96
BsrBI CCGCTC 1 cut(s) 232
BsrI ACTGG 1 cut(s) 150
BssECI CCNNGG 1 cut(s) 171
BssMI GATC 4 cut(s) 6, 10, 52, 271
BssSI CACGAG 1 cut(s) 158
BssT1I CCWWGG 1 cut(s) 171
Bst2BI CACGAG 1 cut(s) 158
BstAPI GCANNNNNTGC 1 cut(s) 92
BstC8I GCNNGC 2 cut(s) 230, 248
BstDEI CTNAG 3 cut(s) 78, 145, 329
BstDSI CCRYGG 1 cut(s) 171
BstF5I GGATG 1 cut(s) 208
BstKTI GATC 4 cut(s) 9, 13, 55, 274
BstMAI GTCTC 1 cut(s) 96
BstMBI GATC 4 cut(s) 6, 10, 52, 271
BstMWI GCNNNNNNNGC 2 cut(s) 83, 92
BstSFI CTRYAG 3 cut(s) 276, 307, 364
BstSLI GKGCMC 1 cut(s) 337
BstV1I GCAGC 2 cut(s) 82, 237
Bsu15I ATCGAT 1 cut(s) 9
BsuRI GGCC 2 cut(s) 170, 303
BsuTUI ATCGAT 1 cut(s) 9
BtgI CCRYGG 1 cut(s) 171
BtsCI GGATG 1 cut(s) 208
BtsI GCAGTG 1 cut(s) 335
BtsIMutI CAGTG 2 cut(s) 157, 335
Cac8I GCNNGC 2 cut(s) 230, 248
Cfr13I GGNCC 3 cut(s) 293, 319, 360
ClaI ATCGAT 1 cut(s) 9
CviAII CATG 1 cut(s) 172
DdeI CTNAG 3 cut(s) 78, 145, 329
DpnI GATC 4 cut(s) 8, 12, 54, 273
DpnII GATC 4 cut(s) 6, 10, 52, 271
EaeI YGGCCR 1 cut(s) 301
Ecl136II GAGCTC 1 cut(s) 64
Eco130I CCWWGG 1 cut(s) 171
Eco24I GRGCYC 2 cut(s) 66, 230
Eco31I GGTCTC 1 cut(s) 96
Eco47I GGWCC 3 cut(s) 293, 319, 360
Eco53kI GAGCTC 1 cut(s) 64
Eco88I CYCGRG 1 cut(s) 254
EcoICRI GAGCTC 1 cut(s) 64
EcoO109I RGGNCCY 2 cut(s) 293, 319
EcoT14I CCWWGG 1 cut(s) 171
EcoT38I GRGCYC 2 cut(s) 66, 230
ErhI CCWWGG 1 cut(s) 171
FaeI CATG 1 cut(s) 175
FaiI YATR 7 cut(s) 93, 131, 173, 222, 278, 366, 385
FatI CATG 1 cut(s) 171
FauI CCCGC 1 cut(s) 237
Fnu4HI GCNGC 4 cut(s) 96, 244, 251, 304
FokI GGATG 1 cut(s) 215
FriOI GRGCYC 2 cut(s) 66, 230
Fsp4HI GCNGC 4 cut(s) 96, 244, 251, 304
FspBI CTAG 2 cut(s) 348, 397
GluI GCNGC 4 cut(s) 96, 244, 251, 304
HaeIII GGCC 2 cut(s) 170, 303
Hin1II CATG 1 cut(s) 175
Hpy166II GTNNAC 1 cut(s) 155
Hpy188I TCNGA 1 cut(s) 22
Hpy188III TCNNGA 2 cut(s) 56, 256
Hpy8I GTNNAC 1 cut(s) 155
HpyAV CCTTC 2 cut(s) 76, 160
HpyF10VI GCNNNNNNNGC 2 cut(s) 83, 92
HpyF3I CTNAG 3 cut(s) 78, 145, 329
Hsp92II CATG 1 cut(s) 175
Kzo9I GATC 4 cut(s) 6, 10, 52, 271
LmnI GCTCC 3 cut(s) 23, 69, 237
LpnPI CCDG 6 cut(s) 87, 112, 163, 260, 309, 316
Lsp1109I GCAGC 2 cut(s) 82, 237
LweI GCATC 1 cut(s) 193
MaeI CTAG 2 cut(s) 348, 397
MaeIII GTNAC 1 cut(s) 68
MalI GATC 4 cut(s) 8, 12, 54, 273
MbiI CCGCTC 1 cut(s) 232
MboI GATC 4 cut(s) 6, 10, 52, 271
MboII GAAGA 2 cut(s) 133, 193
MhlI GDGCHC 4 cut(s) 66, 141, 230, 337
MmeI TCCRAC 1 cut(s) 338
MnlI CCTC 4 cut(s) 29, 184, 193, 310
MspA1I CMGCKG 1 cut(s) 250
MwoI GCNNNNNNNGC 2 cut(s) 83, 92
NcoI CCATGG 1 cut(s) 171
NdeII GATC 4 cut(s) 6, 10, 52, 271
NlaIII CATG 1 cut(s) 175
NlaIV GGNNCC 1 cut(s) 321
NmuCI GTSAC 1 cut(s) 68
PaeR7I CTCGAG 1 cut(s) 254
PkrI GCNGC 4 cut(s) 97, 245, 252, 305
PpuMI RGGWCCY 2 cut(s) 293, 319
Psp124BI GAGCTC 1 cut(s) 66
Psp5II RGGWCCY 2 cut(s) 293, 319
PspN4I GGNNCC 1 cut(s) 321
PspPI GGNCC 3 cut(s) 293, 319, 360
PspPPI RGGWCCY 2 cut(s) 293, 319
PvuII CAGCTG 1 cut(s) 250
SacI GAGCTC 1 cut(s) 66
SatI GCNGC 4 cut(s) 96, 244, 251, 304
Sau3AI GATC 4 cut(s) 6, 10, 52, 271
Sau96I GGNCC 3 cut(s) 293, 319, 360
SduI GDGCHC 4 cut(s) 66, 141, 230, 337
SetI ASST 6 cut(s) 20, 66, 195, 252, 298, 365
SfaNI GCATC 1 cut(s) 193
SfcI CTRYAG 3 cut(s) 276, 307, 364
Sfr274I CTCGAG 1 cut(s) 254
SinI GGWCC 3 cut(s) 293, 319, 360
SlaI CTCGAG 1 cut(s) 254
SmlI CTYRAG 2 cut(s) 197, 254
SmoI CTYRAG 2 cut(s) 197, 254
SsiI CCGC 3 cut(s) 230, 244, 304
SspMI CTAG 2 cut(s) 348, 397
SstI GAGCTC 1 cut(s) 66
StyI CCWWGG 1 cut(s) 171
TaqI TCGA 3 cut(s) 9, 135, 255
TauI GCSGC 2 cut(s) 246, 306
TscAI CASTG 2 cut(s) 157, 342
TseFI GTSAC 1 cut(s) 68
TseI GCWGC 2 cut(s) 95, 250
Tsp45I GTSAC 1 cut(s) 68
TspGWI ACGGA 1 cut(s) 56
TspRI CASTG 2 cut(s) 157, 342
VpaK11BI GGWCC 3 cut(s) 293, 319, 360
XhoI CTCGAG 1 cut(s) 254
XspI CTAG 2 cut(s) 348, 397
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.