Rh1AG296900

The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1A
OG0007091
Physical Location & Seq
Forward (+)
52781870 .. 52785076
3207 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1AG296900.1

Sequence Viewer

Length: 1020 bp
ATGGTATTGGGTAGAGCTTTTGAGGACATGTGTGCTCAGATGTATTACAGAGGCAAAATGTTTGGGTTTGTTCACCTTTACAATGGCCAAGAAGCTGTGTCAACCGGGTTCATCAAGCTTACAAAGCAGGAAGATACTGTGGTGAGCACATACCGTGATCATGTTCACTCTTTGAGTAAGGGTGTCCCTGCTCGTGAAGTCATGAGTGAGCTCTTCGGAAAGGCTACCGGTTGTTGCCGGGGCCAAGGTGGTTCGATGCATATGTTCTCAAAAGCCCACAATTTGATTGGTGGGTTTGCATTTATTGGTGAAGGGATTCCAGTGGCAACAGGGGCAGCCTTCAGCTCTAAATATAGGAGGGAAGTGTTGAAACAGGCAGACTGTGATCACGTGACATTGGCATTTTTTGGAGATGGGACTGCTAACAATGGCCAGTTCTTTGAATGTTTGAACATGGCTGCATTATGGAAATTACCAATTGTATTTGTTGTGGAAAACAATTTGTGGGCTATTGGAATGTCGCATCTGAGGGCAACTTCTGATCCCGAAATTTGGAAGAAGGGACCTGCATTTGGTATGCCTGGTGTTCATGTGGATGGGATGGATGTTTTGAAGGTGAGGGAAGTGGCAAAGGAGGCAATTGGAAGGGCCAGGAGAGGAGAGGGACCAACCTTGGTGGAATGTGAAACATATAGGTTTAGAGGACACTCATTGGCTGATCCCGATGAGCTCCGTGACCCTGCTGAGAAGGCACACTATGCTGCCAGAGACCCCATCACAGCCTTGAAGAAATACATATTCGAGCACAACTTAGCCAGTGAACAAGAGCTGAAGGCCATAGAGAAGAAGATAGATGAGGTGCTTGAGGATGCTGTTGAGTTCGCCGATGAGAGCCCGCTCCCACCTCGCAGCCAGCTGCTCGAGAATGTGTTTGCTGATCCTAAAGGCTTTGGAATAGGGCCTGATGGCCGCTACAGATGTGAGGACCCTAAATTTACTCAGGGCACTGCCAAAGTCTAA

Protein Analysis

339

Amino Acids

37.52

Weight (kDa)

6.15

Isoelectric Point (pI)

34.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
E1_dh PF00676 2 - 301 1.1e-82 Dehydrogenase E1 component
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 574
AccBSI CCGCTC 1 cut(s) 898
AciI CCGC 2 cut(s) 896, 970
AclWI GGATC 3 cut(s) 536, 713, 932
AcoI YGGCCR 3 cut(s) 85, 430, 967
AcsI RAATTY 2 cut(s) 549, 992
AcuI CTGAAG 2 cut(s) 325, 851
AcvI CACGTG 1 cut(s) 391
AfiI CCNNNNNNNGG 2 cut(s) 552, 572
AflIII ACRYGT 1 cut(s) 27
AgeI ACCGGT 1 cut(s) 227
AgsI TTSAA 5 cut(s) 370, 443, 451, 613, 787
AjnI CCWGG 2 cut(s) 580, 650
AluBI AGCT 8 cut(s) 17, 95, 118, 211, 345, 730, 829, 916
AluI AGCT 8 cut(s) 17, 95, 118, 211, 345, 730, 829, 916
Alw21I GWGCWC 5 cut(s) 37, 149, 213, 732, 807
Alw26I GTCTC 1 cut(s) 762
AlwI GGATC 3 cut(s) 536, 713, 932
Ama87I CYCGRG 1 cut(s) 920
AoxI GGCC 7 cut(s) 85, 241, 430, 648, 834, 959, 967
ApeKI GCWGC 5 cut(s) 335, 458, 761, 909, 916
ApoI RAATTY 2 cut(s) 549, 992
AsiGI ACCGGT 1 cut(s) 227
Asp700I GAANNNNTTC 1 cut(s) 315
AspS9I GGNCC 6 cut(s) 241, 563, 648, 665, 959, 985
AsuC2I CCSGG 2 cut(s) 106, 239
AsuHPI GGTGA 4 cut(s) 65, 154, 320, 628
AvaI CYCGRG 1 cut(s) 920
AvaII GGWCC 3 cut(s) 563, 665, 985
BaeGI GKGCMC 1 cut(s) 1007
BaeI ACNNNNGTAYC 2 cut(s) 126, 159
BalI TGGCCA 2 cut(s) 87, 432
BanII GRGCYC 3 cut(s) 213, 732, 896
BauI CACGAG 1 cut(s) 192
BbrPI CACGTG 1 cut(s) 391
Bbv12I GWGCWC 5 cut(s) 37, 149, 213, 732, 807
BbvI GCAGC 5 cut(s) 347, 445, 748, 903, 921
BccI CCATC 5 cut(s) 407, 590, 595, 782, 959
BciT130I CCWGG 2 cut(s) 582, 652
BclI TGATCA 2 cut(s) 157, 385
BcnI CCSGG 2 cut(s) 106, 239
BcoDI GTCTC 1 cut(s) 762
BfmI CTRYAG 1 cut(s) 973
BfuAI ACCTGC 1 cut(s) 574
BisI GCNGC 6 cut(s) 336, 459, 762, 910, 917, 970
BlsI GCNGC 6 cut(s) 337, 460, 763, 911, 918, 971
Bme1390I CCNGG 4 cut(s) 106, 239, 582, 652
Bme18I GGWCC 3 cut(s) 563, 665, 985
BmeT110I CYCGRG 1 cut(s) 920
BmgT120I GGNCC 6 cut(s) 241, 563, 648, 665, 959, 985
BmiI GGNNCC 4 cut(s) 242, 564, 666, 987
BmrFI CCNGG 4 cut(s) 106, 239, 582, 652
BmsI GCATC 3 cut(s) 246, 532, 859
BpuEI CTTGAG 1 cut(s) 884
BpuMI CCSGG 2 cut(s) 106, 239
BsaAI YACGTR 1 cut(s) 391
BsaI GGTCTC 1 cut(s) 762
BsaJI CCNNGG 3 cut(s) 238, 244, 672
BsaWI WCCGGW 1 cut(s) 227
Bsc4I CCNNNNNNNGG 2 cut(s) 552, 572
Bse118I RCCGGY 1 cut(s) 227
Bse1I ACTGG 3 cut(s) 320, 433, 816
BseBI CCWGG 2 cut(s) 582, 652
BseDI CCNNGG 3 cut(s) 238, 244, 672
BseGI GGATG 4 cut(s) 601, 606, 610, 874
BseLI CCNNNNNNNGG 2 cut(s) 552, 572
BseMII CTCAG 4 cut(s) 50, 518, 735, 1013
BseNI ACTGG 3 cut(s) 320, 433, 816
BseRI GAGGAG 1 cut(s) 672
BseSI GKGCMC 1 cut(s) 1007
BseXI GCAGC 5 cut(s) 347, 445, 748, 903, 921
BshFI GGCC 7 cut(s) 87, 243, 432, 650, 836, 961, 969
BshTI ACCGGT 1 cut(s) 227
BsiHKAI GWGCWC 5 cut(s) 37, 149, 213, 732, 807
BsiHKCI CYCGRG 1 cut(s) 920
BsiSI CCGG 3 cut(s) 105, 228, 238
BslFI GGGAC 4 cut(s) 170, 430, 576, 678
BslI CCNNNNNNNGG 2 cut(s) 552, 572
BsmAI GTCTC 1 cut(s) 762
BsmFI GGGAC 4 cut(s) 170, 430, 576, 678
BsnI GGCC 7 cut(s) 87, 243, 432, 650, 836, 961, 969
Bso31I GGTCTC 1 cut(s) 762
BsoBI CYCGRG 1 cut(s) 920
Bsp1286I GDGCHC 7 cut(s) 37, 149, 213, 732, 807, 896, 1007
Bsp143I GATC 5 cut(s) 157, 385, 541, 718, 937
BspACI CCGC 2 cut(s) 896, 970
BspANI GGCC 7 cut(s) 87, 243, 432, 650, 836, 961, 969
BspCNI CTCAG 4 cut(s) 49, 519, 736, 1012
BspHI TCATGA 1 cut(s) 201
BspLI GGNNCC 4 cut(s) 242, 564, 666, 987
BspMI ACCTGC 1 cut(s) 574
BspPI GGATC 3 cut(s) 536, 713, 932
BspQI GCTCTTC 1 cut(s) 218
BspTNI GGTCTC 1 cut(s) 762
BsrBI CCGCTC 1 cut(s) 898
BsrFI RCCGGY 1 cut(s) 227
BsrI ACTGG 3 cut(s) 320, 433, 816
BssAI RCCGGY 1 cut(s) 227
BssECI CCNNGG 3 cut(s) 238, 244, 672
BssMI GATC 5 cut(s) 157, 385, 541, 718, 937
BssSI CACGAG 1 cut(s) 192
BssT1I CCWWGG 2 cut(s) 244, 672
Bst2BI CACGAG 1 cut(s) 192
Bst2UI CCWGG 2 cut(s) 582, 652
Bst4CI ACNGT 3 cut(s) 139, 155, 383
Bst6I CTCTTC 1 cut(s) 218
BstAPI GCANNNNNTGC 1 cut(s) 758
BstBAI YACGTR 1 cut(s) 391
BstC8I GCNNGC 2 cut(s) 896, 914
BstDEI CTNAG 5 cut(s) 36, 527, 744, 811, 999
BstF5I GGATG 4 cut(s) 601, 606, 610, 874
BstKTI GATC 5 cut(s) 160, 388, 544, 721, 940
BstMAI GTCTC 1 cut(s) 762
BstMBI GATC 5 cut(s) 157, 385, 541, 718, 937
BstMWI GCNNNNNNNGC 5 cut(s) 124, 332, 635, 749, 758
BstNI CCWGG 2 cut(s) 582, 652
BstNSI RCATGY 1 cut(s) 31
BstSCI CCNGG 4 cut(s) 104, 237, 580, 650
BstSFI CTRYAG 1 cut(s) 973
BstSLI GKGCMC 1 cut(s) 1007
BstV1I GCAGC 5 cut(s) 347, 445, 748, 903, 921
BsuRI GGCC 7 cut(s) 87, 243, 432, 650, 836, 961, 969
BtsCI GGATG 4 cut(s) 601, 606, 610, 874
BtsI GCAGTG 1 cut(s) 1005
BtsIMutI CAGTG 3 cut(s) 327, 823, 1005
BveI ACCTGC 1 cut(s) 574
Cac8I GCNNGC 2 cut(s) 896, 914
CciI TCATGA 1 cut(s) 201
Cfr10I RCCGGY 1 cut(s) 227
Cfr13I GGNCC 6 cut(s) 241, 563, 648, 665, 959, 985
CspAI ACCGGT 1 cut(s) 227
CspCI CAANNNNNGTGG 2 cut(s) 657, 692
CviAII CATG 5 cut(s) 28, 161, 202, 454, 590
DdeI CTNAG 5 cut(s) 36, 527, 744, 811, 999
DpnI GATC 5 cut(s) 159, 387, 543, 720, 939
DpnII GATC 5 cut(s) 157, 385, 541, 718, 937
EaeI YGGCCR 3 cut(s) 85, 430, 967
Eam1104I CTCTTC 1 cut(s) 218
EarI CTCTTC 1 cut(s) 218
Ecl136II GAGCTC 2 cut(s) 211, 730
Eco130I CCWWGG 2 cut(s) 244, 672
Eco24I GRGCYC 3 cut(s) 213, 732, 896
Eco31I GGTCTC 1 cut(s) 762
Eco47I GGWCC 3 cut(s) 563, 665, 985
Eco53kI GAGCTC 2 cut(s) 211, 730
Eco57I CTGAAG 2 cut(s) 325, 851
Eco72I CACGTG 1 cut(s) 391
Eco88I CYCGRG 1 cut(s) 920
EcoICRI GAGCTC 2 cut(s) 211, 730
EcoO109I RGGNCCY 3 cut(s) 563, 959, 985
EcoRII CCWGG 2 cut(s) 580, 650
EcoT14I CCWWGG 2 cut(s) 244, 672
EcoT22I ATGCAT 1 cut(s) 261
EcoT38I GRGCYC 3 cut(s) 213, 732, 896
ErhI CCWWGG 2 cut(s) 244, 672
FaeI CATG 5 cut(s) 31, 164, 205, 457, 593
FaqI GGGAC 4 cut(s) 170, 430, 576, 678
FatI CATG 5 cut(s) 27, 160, 201, 453, 589
FauI CCCGC 1 cut(s) 903
FauNDI CATATG 1 cut(s) 261
FbaI TGATCA 2 cut(s) 157, 385
Fnu4HI GCNGC 6 cut(s) 336, 459, 762, 910, 917, 970
FokI GGATG 4 cut(s) 608, 613, 617, 881
FriOI GRGCYC 3 cut(s) 213, 732, 896
Fsp4HI GCNGC 6 cut(s) 336, 459, 762, 910, 917, 970
GluI GCNGC 6 cut(s) 336, 459, 762, 910, 917, 970
HaeIII GGCC 7 cut(s) 87, 243, 432, 650, 836, 961, 969
HapII CCGG 3 cut(s) 105, 228, 238
Hin1II CATG 5 cut(s) 31, 164, 205, 457, 593
HincII GTYRAC 1 cut(s) 102
HindII GTYRAC 1 cut(s) 102
HindIII AAGCTT 1 cut(s) 116
HinfI GANTC 1 cut(s) 316
HpaII CCGG 3 cut(s) 105, 228, 238
HphI GGTGA 4 cut(s) 65, 154, 320, 628
Hpy166II GTNNAC 4 cut(s) 73, 102, 166, 821
Hpy188I TCNGA 4 cut(s) 39, 218, 528, 541
Hpy188III TCNNGA 5 cut(s) 194, 202, 545, 722, 922
Hpy8I GTNNAC 4 cut(s) 73, 102, 166, 821
HpyAV CCTTC 7 cut(s) 305, 349, 553, 607, 639, 742, 826
HpyCH4III ACNGT 3 cut(s) 139, 155, 383
HpyCH4IV ACGT 1 cut(s) 390
HpyCH4V TGCA 4 cut(s) 259, 299, 461, 569
HpyF10VI GCNNNNNNNGC 5 cut(s) 124, 332, 635, 749, 758
HpyF3I CTNAG 5 cut(s) 36, 527, 744, 811, 999
HpySE526I ACGT 1 cut(s) 390
Hsp92II CATG 5 cut(s) 31, 164, 205, 457, 593
Ksp22I TGATCA 2 cut(s) 157, 385
Kzo9I GATC 5 cut(s) 157, 385, 541, 718, 937
LguI GCTCTTC 1 cut(s) 218
LmnI GCTCC 2 cut(s) 735, 903
Lsp1109I GCAGC 5 cut(s) 347, 445, 748, 903, 921
LweI GCATC 3 cut(s) 246, 532, 859
MaeII ACGT 1 cut(s) 390
MaeIII GTNAC 2 cut(s) 391, 734
MalI GATC 5 cut(s) 159, 387, 543, 720, 939
MbiI CCGCTC 1 cut(s) 898
MboI GATC 5 cut(s) 157, 385, 541, 718, 937
MboII GAAGA 6 cut(s) 143, 205, 568, 799, 856, 859
MfeI CAATTG 2 cut(s) 477, 639
MhlI GDGCHC 7 cut(s) 37, 149, 213, 732, 807, 896, 1007
MlsI TGGCCA 2 cut(s) 87, 432
MluCI AATT 7 cut(s) 280, 470, 477, 499, 549, 639, 992
MluNI TGGCCA 2 cut(s) 87, 432
Mox20I TGGCCA 2 cut(s) 87, 432
Mph1103I ATGCAT 1 cut(s) 261
MroXI GAANNNNTTC 1 cut(s) 315
MscI TGGCCA 2 cut(s) 87, 432
MslI CAYNNNNRTG 1 cut(s) 594
Msp20I TGGCCA 2 cut(s) 87, 432
MspA1I CMGCKG 1 cut(s) 916
MspI CCGG 3 cut(s) 105, 228, 238
MspR9I CCNGG 4 cut(s) 106, 239, 582, 652
MunI CAATTG 2 cut(s) 477, 639
MvaI CCWGG 2 cut(s) 582, 652
MwoI GCNNNNNNNGC 5 cut(s) 124, 332, 635, 749, 758
NciI CCSGG 2 cut(s) 106, 239
NdeI CATATG 1 cut(s) 261
NdeII GATC 5 cut(s) 157, 385, 541, 718, 937
NlaIII CATG 5 cut(s) 31, 164, 205, 457, 593
NlaIV GGNNCC 4 cut(s) 242, 564, 666, 987
NmuCI GTSAC 2 cut(s) 391, 734
NsiI ATGCAT 1 cut(s) 261
NspI RCATGY 1 cut(s) 31
PaeR7I CTCGAG 1 cut(s) 920
PagI TCATGA 1 cut(s) 201
PciI ACATGT 1 cut(s) 27
PciSI GCTCTTC 1 cut(s) 218
PdmI GAANNNNTTC 1 cut(s) 315
PfeI GAWTC 1 cut(s) 316
PinAI ACCGGT 1 cut(s) 227
PkrI GCNGC 6 cut(s) 337, 460, 763, 911, 918, 971
PmaCI CACGTG 1 cut(s) 391
PmlI CACGTG 1 cut(s) 391
Ppu21I YACGTR 1 cut(s) 391
PpuMI RGGWCCY 2 cut(s) 563, 985
PscI ACATGT 1 cut(s) 27
Psp124BI GAGCTC 2 cut(s) 213, 732
Psp5II RGGWCCY 2 cut(s) 563, 985
Psp6I CCWGG 2 cut(s) 580, 650
PspCI CACGTG 1 cut(s) 391
PspGI CCWGG 2 cut(s) 580, 650
PspN4I GGNNCC 4 cut(s) 242, 564, 666, 987
PspPI GGNCC 6 cut(s) 241, 563, 648, 665, 959, 985
PspPPI RGGWCCY 2 cut(s) 563, 985
PvuII CAGCTG 1 cut(s) 916
RseI CAYNNNNRTG 1 cut(s) 594
SacI GAGCTC 2 cut(s) 213, 732
SapI GCTCTTC 1 cut(s) 218
SatI GCNGC 6 cut(s) 336, 459, 762, 910, 917, 970
Sau3AI GATC 5 cut(s) 157, 385, 541, 718, 937
Sau96I GGNCC 6 cut(s) 241, 563, 648, 665, 959, 985
ScrFI CCNGG 4 cut(s) 106, 239, 582, 652
SduI GDGCHC 7 cut(s) 37, 149, 213, 732, 807, 896, 1007
SfaNI GCATC 3 cut(s) 246, 532, 859
SfcI CTRYAG 1 cut(s) 973
Sfr274I CTCGAG 1 cut(s) 920
SinI GGWCC 3 cut(s) 563, 665, 985
SlaI CTCGAG 1 cut(s) 920
SmiMI CAYNNNNRTG 1 cut(s) 594
SmlI CTYRAG 2 cut(s) 863, 920
SmoI CTYRAG 2 cut(s) 863, 920
Sse9I AATT 7 cut(s) 280, 470, 477, 499, 549, 639, 992
SsiI CCGC 2 cut(s) 896, 970
SstI GAGCTC 2 cut(s) 213, 732
StyD4I CCNGG 4 cut(s) 104, 237, 580, 650
StyI CCWWGG 2 cut(s) 244, 672
TaaI ACNGT 3 cut(s) 139, 155, 383
TaiI ACGT 1 cut(s) 393
TaqI TCGA 3 cut(s) 254, 801, 921
TasI AATT 7 cut(s) 280, 470, 477, 499, 549, 639, 992
TauI GCSGC 1 cut(s) 972
TfiI GAWTC 1 cut(s) 316
TscAI CASTG 3 cut(s) 327, 823, 1012
TseFI GTSAC 2 cut(s) 391, 734
TseI GCWGC 5 cut(s) 335, 458, 761, 909, 916
Tsp45I GTSAC 2 cut(s) 391, 734
TspDTI ATGAA 2 cut(s) 100, 578
TspGWI ACGGA 1 cut(s) 722
TspRI CASTG 3 cut(s) 327, 823, 1012
VpaK11BI GGWCC 3 cut(s) 563, 665, 985
XapI RAATTY 2 cut(s) 549, 992
XceI RCATGY 1 cut(s) 31
XcmI CCANNNNNNNNNTGG 1 cut(s) 284
XhoI CTCGAG 1 cut(s) 920
XmnI GAANNNNTTC 1 cut(s) 315
Zsp2I ATGCAT 1 cut(s) 261
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.