Rmu_ssc0000450.1_g000001

Structural maintenance of chromosomes protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_ssc0000450.1
OG0003625
Physical Location & Seq
Forward (+)
1 .. 10830
10830 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 2774 bp
gaagaaaggttgctagtcggaaggaggacctccgggagctaattgaacattttagtattgatgttgagaatccatgtgtaataatgggccaagacagaagcagagagtttttgcattctgggaatgacaaagataaatttaagttcttttacaaggccacacttctgcagcaagttgaagagcttttgcaagacattgagaaacagttggagaaggcaaatgttgttgttgatcaattagagggctcaataagacccatagaaagggaacttaatgagttacatgaaaagatcaaaaacatggagcacattgaagaaatatctcaacaagcgaagcagctaaaaaagaagcttgcttggtcatgggtatatgatgtagacaggcaactccagcaaaagaatgcaaaaattggaaagctgaaaaatcgtgtacctctttgtcaagctaaaattgatcagtttagaggtgaagtggagaaacttagccaatgctataccttgaagaaatctgagattgcaagcatggtggaaaagaccgctgaagttaggagaatgaaggacgaattgcgacagtcgctagcatcggctacaaaagagaagcttaagctagaagaagaatatggccgcaaaactaactacatccagaaattgacaaactctgttaggtctcttcagcaacagattcaggatgctgaggagcagcatgccagaaatacacaggcagaagaatctgcaatggaggaaaagctgaaagaactgcataatgaggtcgctactattgaatcaatgcttgcaaggttgcaggaagaagacagtgccttatcaataagtgtacacaagacaattactgaaattggaaaattaactgaaatgattcaaagcagagagaaggaatgtcaaaaatactccaataaagtccgcgaggttttgagaaatcaaagtaacaaggtcacagcttttggtggagatagagtgatggaccttctacgtataattgagaggaatcatcagagattcaaaagccctccaattggtccgattggtgcccatttgactttgaataatggagatgaatgggctagaactgttgaaaatgctgtaggaaggctgctcaatgctttcattgtaacaaatcataaagactctcaacttctcagaacatgtgcaagggaagcaagctataatcaccttcagattatcatatatgacttttcattaccaaggttgaatattccacctcacatgcttccacaaacaagacacccaactactctttctcttctccattctgaaaatcatactgtggtaaatgtcctggtagatatgggaagtgttgaaaggcaagttcttgttaaaacatatgaggaggggaaggaagttgcttttgaccagaggatccccaatctgaaggaggtctacacttatgatgggcgaaagatgttttcgcgtggttctgtccagacagttcttcctcccattaggcatgatagacctgctcgtctctgtgctaattatgatgatcaaatcaatgagcttaaaacatcagcatcagctgtacaaaaagaagctgaggagtgcaggacgagaaaaagggaagaagaagacaaactttggaatctcaaagaaaaacaccaaagtgtgaagaggaggcgtatgaatgctgaccgtgatttggcgtctaagagactagctattcaagacagtgcatatgatgcggaagccagtacatcacctgcatcaactgttgatgaccttcatcaagacattttgaaagtccaagaggagattcaagagagagaaatgtcgctgaaaacctttgaagtcagaattagtgaagctgtggcgaagaccaatgatcttaaagtaacatttgagaatctatgtgagtcagcaaaaggagacattgatgcctttgagaaagcagagagagatttgatggagatagaaacaaaactaggttctgcgcaaacggagaagcttcattatgaaggtctaatgaagaccaaggtccttcatgaaatcaatgacgctgaaaaacaatataaagagcttgagcatcaccgcgaggacaactgtagaaaggcttcaatcctctgtcctgaaagtgaaattacagctttgggtgattgggatggtagcactccagaacaactcagtgcccaattaaccaggctgaatcagagacttcagcgagagagtgagagatgcactgaatccattgatgagctccgaatgtcatatgagagaaaagaacgtaagattttaagaaaacagaaaacatacagagcttttcgagaaaagttgaatgcttgccagaaagccctaaagttgcgaagtacaaagttccagagaaataaagatttactaaagcgccagatgacgtggcgatttaattctcatctgggaaggaaagggttcagtgggaagattaaggtcagttatgaggagagaaccctttcaattgaggtaaagatgccccaggattcatcaagcagcactgttcgcgacactagagggctttcaggtggagaacgctccttctcaacattgtgctttgcgctggctcttcatgatatgactgaagccccatttagagctatggatgagtttgatgtatttatggatgctgtaagtcggaaaatcagccttgatactcttgttgagtttgcattggcgcaaggatcccaatgggtacttatcacccctcatgatatcagcatggtgaaaaatggggatcggataaagaagcagcaaatggcggctcctcgttcttga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AAA_23 SMC_N

Protein Analysis

923

Amino Acids

106.94

Weight (kDa)

8.41

Isoelectric Point (pI)

48.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SMC_N PF02463 224 - 916 6.9e-10 RecF/RecN/SMC N terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1740
AasI GACNNNNNNGTC 1 cut(s) 1496
Acc16I TGCGCA 1 cut(s) 1964
Acc36I ACCTGC 2 cut(s) 1500, 1740
AccB1I GGYRCC 1 cut(s) 1042
AccI GTMKAC 2 cut(s) 377, 1415
AccII CGCG 4 cut(s) 920, 1446, 2063, 2504
AciI CCGC 6 cut(s) 536, 624, 918, 1714, 2061, 2758
AclWI GGATC 5 cut(s) 1389, 1402, 2675, 2688, 2741
AcoI YGGCCR 1 cut(s) 621
AcsI RAATTY 1 cut(s) 136
AcuI CTGAAG 6 cut(s) 560, 655, 1174, 1426, 2170, 2600
AcyI GRCGYC 1 cut(s) 1675
AfaI GTAC 6 cut(s) 431, 833, 1556, 1725, 2337, 2693
AfiI CCNNNNNNNGG 3 cut(s) 263, 1030, 1671
AflII CTTAAG 1 cut(s) 601
AflIII ACRYGT 1 cut(s) 1159
AjiI CACGTC 1 cut(s) 2381
AjnI CCWGG 3 cut(s) 1313, 2167, 2477
AjuI GAANNNNNNNTTGG 4 cut(s) 1257, 1289, 1769, 1801
AleI CACNNNNGTG 1 cut(s) 1634
Alw21I GWGCWC 2 cut(s) 308, 2228
Alw26I GTCTC 5 cut(s) 671, 1504, 1677, 1892, 2175
AlwI GGATC 5 cut(s) 1389, 1402, 2675, 2688, 2741
AoxI GGCC 3 cut(s) 87, 155, 621
ApeKI GCWGC 6 cut(s) 168, 336, 699, 1107, 2492, 2748
ApoI RAATTY 1 cut(s) 136
Asp700I GAANNNNTTC 4 cut(s) 872, 2082, 2413, 2454
AspLEI GCGC 4 cut(s) 1965, 2372, 2559, 2676
AspS9I GGNCC 5 cut(s) 27, 87, 978, 1033, 2007
AsuC2I CCSGG 1 cut(s) 34
AsuHPI GGTGA 7 cut(s) 478, 1177, 1721, 2050, 2134, 2691, 2733
AsuNHI GCTAGC 1 cut(s) 576
AvaII GGWCC 4 cut(s) 27, 978, 1033, 2007
BaeGI GKGCMC 2 cut(s) 1047, 2160
BaeI ACNNNNGTAYC 2 cut(s) 2642, 2675
BamHI GGATCC 2 cut(s) 1394, 2680
BanI GGYRCC 1 cut(s) 1042
BanII GRGCYC 2 cut(s) 247, 2228
BarI GAAGNNNNNNTAC 4 cut(s) 1399, 1431, 2067, 2099
BbsI GAAGAC 4 cut(s) 815, 1607, 1852, 2005
Bbv12I GWGCWC 2 cut(s) 308, 2228
BbvCI CCTCAGC 2 cut(s) 692, 1568
BbvI GCAGC 6 cut(s) 180, 348, 711, 1094, 2504, 2760
BccI CCATC 4 cut(s) 969, 1420, 1929, 2125
BciT130I CCWGG 3 cut(s) 1315, 2169, 2479
BclI TGATCA 3 cut(s) 231, 453, 1518
BcnI CCSGG 1 cut(s) 34
BcoDI GTCTC 5 cut(s) 671, 1504, 1677, 1892, 2175
BfaI CTAG 7 cut(s) 14, 577, 607, 1079, 1687, 1954, 2510
BfmI CTRYAG 3 cut(s) 166, 1097, 2073
BfoI RGCGCY 1 cut(s) 2373
BfrI CTTAAG 1 cut(s) 601
BfuAI ACCTGC 2 cut(s) 1500, 1740
BisI GCNGC 8 cut(s) 169, 337, 624, 700, 1108, 2493, 2749, 2759
BlsI GCNGC 8 cut(s) 170, 338, 625, 701, 1109, 2494, 2750, 2760
Bme1390I CCNGG 4 cut(s) 34, 1315, 2169, 2479
Bme18I GGWCC 4 cut(s) 27, 978, 1033, 2007
BmgBI CACGTC 1 cut(s) 2381
BmgT120I GGNCC 5 cut(s) 27, 87, 978, 1033, 2007
BmiI GGNNCC 4 cut(s) 1044, 1396, 2682, 2762
BmrFI CCNGG 4 cut(s) 34, 1315, 2169, 2479
BmtI GCTAGC 1 cut(s) 580
BoxI GACNNNNGTC 1 cut(s) 2005
BpiI GAAGAC 4 cut(s) 815, 1607, 1852, 2005
BpmI CTGGAG 2 cut(s) 373, 2126
Bpu10I CCTNAGC 2 cut(s) 692, 1568
BpuEI CTTGAG 1 cut(s) 2071
BpuMI CCSGG 1 cut(s) 34
BsaAI YACGTR 1 cut(s) 988
BsaHI GRCGYC 1 cut(s) 1675
BsaI GGTCTC 1 cut(s) 671
BsaJI CCNNGG 3 cut(s) 1219, 2003, 2477
BsaXI ACNNNNNCTCC 2 cut(s) 956, 986
Bsc4I CCNNNNNNNGG 3 cut(s) 263, 1030, 1671
Bse1I ACTGG 1 cut(s) 1721
Bse3DI GCAATG 1 cut(s) 740
BseBI CCWGG 3 cut(s) 1315, 2169, 2479
BseDI CCNNGG 3 cut(s) 1219, 2003, 2477
BseGI GGATG 5 cut(s) 638, 693, 2136, 2607, 2628
BseLI CCNNNNNNNGG 3 cut(s) 263, 1030, 1671
BseMI GCAATG 1 cut(s) 740
BseMII CTCAG 5 cut(s) 500, 683, 1167, 1559, 2166
BseNI ACTGG 1 cut(s) 1721
BseRI GAGGAG 7 cut(s) 709, 1378, 1585, 1659, 1795, 2458, 2753
BseSI GKGCMC 2 cut(s) 1047, 2160
BseXI GCAGC 6 cut(s) 180, 348, 711, 1094, 2504, 2760
BsgI GTGCAG 1 cut(s) 1596
Bsh1236I CGCG 4 cut(s) 920, 1446, 2063, 2504
BshFI GGCC 3 cut(s) 89, 157, 623
BshNI GGYRCC 1 cut(s) 1042
BsiHKAI GWGCWC 2 cut(s) 308, 2228
BsiSI CCGG 1 cut(s) 33
BslI CCNNNNNNNGG 3 cut(s) 263, 1030, 1671
BsmAI GTCTC 5 cut(s) 671, 1504, 1677, 1892, 2175
BsmBI CGTCTC 1 cut(s) 1504
BsmI GAATGC 4 cut(s) 114, 405, 1662, 2310
BsnI GGCC 3 cut(s) 89, 157, 623
Bso31I GGTCTC 1 cut(s) 671
Bsp1286I GDGCHC 5 cut(s) 247, 308, 1047, 2160, 2228
Bsp1407I TGTACA 2 cut(s) 831, 1554
Bsp143I GATC 8 cut(s) 231, 290, 453, 1394, 1518, 1854, 2680, 2733
Bsp68I TCGCGA 1 cut(s) 2504
BspACI CCGC 6 cut(s) 536, 624, 918, 1714, 2061, 2758
BspANI GGCC 3 cut(s) 89, 157, 623
BspCNI CTCAG 5 cut(s) 501, 684, 1166, 1560, 2165
BspFNI CGCG 4 cut(s) 920, 1446, 2063, 2504
BspHI TCATGA 3 cut(s) 2013, 2568, 2706
BspLI GGNNCC 4 cut(s) 1044, 1396, 2682, 2762
BspMAI CTGCAG 1 cut(s) 170
BspMI ACCTGC 2 cut(s) 1500, 1740
BspOI GCTAGC 1 cut(s) 580
BspPI GGATC 5 cut(s) 1389, 1402, 2675, 2688, 2741
BspQI GCTCTTC 2 cut(s) 173, 2570
BspT107I GGYRCC 1 cut(s) 1042
BspTI CTTAAG 1 cut(s) 601
BspTNI GGTCTC 1 cut(s) 671
BsrDI GCAATG 1 cut(s) 740
BsrGI TGTACA 2 cut(s) 831, 1554
BsrI ACTGG 1 cut(s) 1721
BssECI CCNNGG 3 cut(s) 1219, 2003, 2477
BssMI GATC 8 cut(s) 231, 290, 453, 1394, 1518, 1854, 2680, 2733
BssNI GRCGYC 1 cut(s) 1675
BssT1I CCWWGG 2 cut(s) 1219, 2003
Bst2UI CCWGG 3 cut(s) 1315, 2169, 2479
Bst6I CTCTTC 5 cut(s) 173, 674, 1283, 1636, 2570
BstACI GRCGYC 1 cut(s) 1675
BstAFI CTTAAG 1 cut(s) 601
BstAPI GCANNNNNTGC 1 cut(s) 1711
BstAUI TGTACA 2 cut(s) 831, 1554
BstBAI YACGTR 1 cut(s) 988
BstC8I GCNNGC 8 cut(s) 353, 519, 578, 704, 791, 1176, 2310, 2561
BstDEI CTNAG 7 cut(s) 481, 509, 692, 1153, 1568, 1679, 2152
BstF5I GGATG 5 cut(s) 638, 693, 2136, 2607, 2628
BstFNI CGCG 4 cut(s) 920, 1446, 2063, 2504
BstH2I RGCGCY 1 cut(s) 2373
BstHHI GCGC 4 cut(s) 1965, 2372, 2559, 2676
BstKTI GATC 8 cut(s) 234, 293, 456, 1397, 1521, 1857, 2683, 2736
BstMAI GTCTC 5 cut(s) 671, 1504, 1677, 1892, 2175
BstMBI GATC 8 cut(s) 231, 290, 453, 1394, 1518, 1854, 2680, 2733
BstMWI GCNNNNNNNGC 5 cut(s) 390, 573, 1171, 1711, 2501
BstNI CCWGG 3 cut(s) 1315, 2169, 2479
BstNSI RCATGY 3 cut(s) 706, 1163, 1245
BstPAI GACNNNNGTC 1 cut(s) 2005
BstSCI CCNGG 4 cut(s) 32, 1313, 2167, 2477
BstSFI CTRYAG 3 cut(s) 166, 1097, 2073
BstSLI GKGCMC 2 cut(s) 1047, 2160
BstSNI TACGTA 1 cut(s) 988
BstUI CGCG 4 cut(s) 920, 1446, 2063, 2504
BstV1I GCAGC 6 cut(s) 180, 348, 711, 1094, 2504, 2760
BstV2I GAAGAC 4 cut(s) 815, 1607, 1852, 2005
BstX2I RGATCY 2 cut(s) 1394, 2680
BstYI RGATCY 2 cut(s) 1394, 2680
BsuRI GGCC 3 cut(s) 89, 157, 623
BtrI CACGTC 1 cut(s) 2381
BtsCI GGATG 5 cut(s) 638, 693, 2136, 2607, 2628
BtsIMutI CAGTG 6 cut(s) 819, 1707, 2160, 2207, 2424, 2495
BtuMI TCGCGA 1 cut(s) 2504
BveI ACCTGC 2 cut(s) 1500, 1740
Cac8I GCNNGC 8 cut(s) 353, 519, 578, 704, 791, 1176, 2310, 2561
CciI TCATGA 3 cut(s) 2013, 2568, 2706
CfoI GCGC 4 cut(s) 1965, 2372, 2559, 2676
Cfr13I GGNCC 5 cut(s) 27, 87, 978, 1033, 2007
CseI GACGC 2 cut(s) 1664, 2035
Csp6I GTAC 6 cut(s) 430, 832, 1555, 1724, 2336, 2692
CspCI CAANNNNNGTGG 2 cut(s) 506, 541
CviQI GTAC 6 cut(s) 430, 832, 1555, 1724, 2336, 2692
DdeI CTNAG 7 cut(s) 481, 509, 692, 1153, 1568, 1679, 2152
DpnI GATC 8 cut(s) 233, 292, 455, 1396, 1520, 1856, 2682, 2735
DpnII GATC 8 cut(s) 231, 290, 453, 1394, 1518, 1854, 2680, 2733
DrdI GACNNNNNNGTC 1 cut(s) 1496
DseDI GACNNNNNNGTC 1 cut(s) 1496
EaeI YGGCCR 1 cut(s) 621
Eam1104I CTCTTC 5 cut(s) 173, 674, 1283, 1636, 2570
EarI CTCTTC 5 cut(s) 173, 674, 1283, 1636, 2570
Ecl136II GAGCTC 1 cut(s) 2226
Eco105I TACGTA 1 cut(s) 988
Eco130I CCWWGG 2 cut(s) 1219, 2003
Eco24I GRGCYC 2 cut(s) 247, 2228
Eco31I GGTCTC 1 cut(s) 671
Eco32I GATATC 1 cut(s) 2712
Eco47I GGWCC 4 cut(s) 27, 978, 1033, 2007
Eco53kI GAGCTC 1 cut(s) 2226
Eco57I CTGAAG 6 cut(s) 560, 655, 1174, 1426, 2170, 2600
EcoICRI GAGCTC 1 cut(s) 2226
EcoO109I RGGNCCY 2 cut(s) 27, 2007
EcoRII CCWGG 3 cut(s) 1313, 2167, 2477
EcoRV GATATC 1 cut(s) 2712
EcoT14I CCWWGG 2 cut(s) 1219, 2003
EcoT38I GRGCYC 2 cut(s) 247, 2228
ErhI CCWWGG 2 cut(s) 1219, 2003
Esp3I CGTCTC 1 cut(s) 1504
FalI AAGNNNNNCTT 4 cut(s) 584, 616, 1592, 1624
FauNDI CATATG 3 cut(s) 1359, 1707, 2238
FbaI TGATCA 3 cut(s) 231, 453, 1518
FblI GTMKAC 2 cut(s) 377, 1415
Fnu4HI GCNGC 8 cut(s) 169, 337, 624, 700, 1108, 2493, 2749, 2759
FokI GGATG 5 cut(s) 625, 700, 2143, 2614, 2635
FriOI GRGCYC 2 cut(s) 247, 2228
Fsp4HI GCNGC 8 cut(s) 169, 337, 624, 700, 1108, 2493, 2749, 2759
FspBI CTAG 7 cut(s) 14, 577, 607, 1079, 1687, 1954, 2510
FspI TGCGCA 1 cut(s) 1964
GlaI GCGC 4 cut(s) 1964, 2371, 2558, 2675
GluI GCNGC 8 cut(s) 169, 337, 624, 700, 1108, 2493, 2749, 2759
GsuI CTGGAG 2 cut(s) 373, 2126
HaeII RGCGCY 1 cut(s) 2373
HaeIII GGCC 3 cut(s) 89, 157, 623
HapII CCGG 1 cut(s) 33
HgaI GACGC 2 cut(s) 1664, 2035
HhaI GCGC 4 cut(s) 1965, 2372, 2559, 2676
Hin1I GRCGYC 1 cut(s) 1675
Hin6I GCGC 4 cut(s) 1963, 2370, 2557, 2674
HinP1I GCGC 4 cut(s) 1963, 2370, 2557, 2674
HindIII AAGCTT 3 cut(s) 349, 598, 1975
HpaII CCGG 1 cut(s) 33
HphI GGTGA 7 cut(s) 478, 1177, 1721, 2050, 2134, 2691, 2733
Hpy166II GTNNAC 5 cut(s) 378, 430, 832, 834, 1416
Hpy8I GTNNAC 5 cut(s) 378, 430, 832, 834, 1416
HpyCH4IV ACGT 3 cut(s) 987, 2253, 2380
HpyF10VI GCNNNNNNNGC 5 cut(s) 390, 573, 1171, 1711, 2501
HpyF3I CTNAG 7 cut(s) 481, 509, 692, 1153, 1568, 1679, 2152
HpySE526I ACGT 3 cut(s) 987, 2253, 2380
Hsp92I GRCGYC 1 cut(s) 1675
HspAI GCGC 4 cut(s) 1963, 2370, 2557, 2674
Ksp22I TGATCA 3 cut(s) 231, 453, 1518
Kzo9I GATC 8 cut(s) 231, 290, 453, 1394, 1518, 1854, 2680, 2733
LguI GCTCTTC 2 cut(s) 173, 2570
LmnI GCTCC 6 cut(s) 36, 303, 696, 2231, 2539, 2766
Lsp1109I GCAGC 6 cut(s) 180, 348, 711, 1094, 2504, 2760
MaeI CTAG 7 cut(s) 14, 577, 607, 1079, 1687, 1954, 2510
MaeII ACGT 3 cut(s) 987, 2253, 2380
MaeIII GTNAC 5 cut(s) 278, 940, 948, 1125, 1863
MalI GATC 8 cut(s) 233, 292, 455, 1396, 1520, 1856, 2682, 2735
MboI GATC 8 cut(s) 231, 290, 453, 1394, 1518, 1854, 2680, 2733
MfeI CAATTG 2 cut(s) 1028, 2459
MflI RGATCY 2 cut(s) 1394, 2680
MhlI GDGCHC 5 cut(s) 247, 308, 1047, 2160, 2228
MlyI GAGTC 2 cut(s) 1135, 1894
MmeI TCCRAC 2 cut(s) 188, 2614
MroXI GAANNNNTTC 4 cut(s) 872, 2082, 2413, 2454
MslI CAYNNNNRTG 1 cut(s) 1634
MspA1I CMGCKG 2 cut(s) 538, 1552
MspCI CTTAAG 1 cut(s) 601
MspI CCGG 1 cut(s) 33
MspR9I CCNGG 4 cut(s) 34, 1315, 2169, 2479
MunI CAATTG 2 cut(s) 1028, 2459
Mva1269I GAATGC 4 cut(s) 114, 405, 1662, 2310
MvaI CCWGG 3 cut(s) 1315, 2169, 2479
MvnI CGCG 4 cut(s) 920, 1446, 2063, 2504
MwoI GCNNNNNNNGC 5 cut(s) 390, 573, 1171, 1711, 2501
NciI CCSGG 1 cut(s) 34
NdeI CATATG 3 cut(s) 1359, 1707, 2238
NdeII GATC 8 cut(s) 231, 290, 453, 1394, 1518, 1854, 2680, 2733
NheI GCTAGC 1 cut(s) 576
NlaIV GGNNCC 4 cut(s) 1044, 1396, 2682, 2762
NmuCI GTSAC 1 cut(s) 948
NruI TCGCGA 1 cut(s) 2504
NsbI TGCGCA 1 cut(s) 1964
NspI RCATGY 3 cut(s) 706, 1163, 1245
OliI CACNNNNGTG 1 cut(s) 1634
PaeI GCATGC 1 cut(s) 706
PagI TCATGA 3 cut(s) 2013, 2568, 2706
PaqCI CACCTGC 1 cut(s) 1740
PciI ACATGT 1 cut(s) 1159
PciSI GCTCTTC 2 cut(s) 173, 2570
PctI GAATGC 4 cut(s) 114, 405, 1662, 2310
PdmI GAANNNNTTC 4 cut(s) 872, 2082, 2413, 2454
PfoI TCCNGGA 1 cut(s) 32
PkrI GCNGC 8 cut(s) 170, 338, 625, 701, 1109, 2494, 2750, 2760
PleI GAGTC 2 cut(s) 1135, 1893
PpsI GAGTC 2 cut(s) 1135, 1893
Ppu21I YACGTR 1 cut(s) 988
PpuMI RGGWCCY 2 cut(s) 27, 2007
PscI ACATGT 1 cut(s) 1159
PshAI GACNNNNGTC 1 cut(s) 2005
Psp124BI GAGCTC 1 cut(s) 2228
Psp5II RGGWCCY 2 cut(s) 27, 2007
Psp6I CCWGG 3 cut(s) 1313, 2167, 2477
PspGI CCWGG 3 cut(s) 1313, 2167, 2477
PspN4I GGNNCC 4 cut(s) 1044, 1396, 2682, 2762
PspPI GGNCC 5 cut(s) 27, 87, 978, 1033, 2007
PspPPI RGGWCCY 2 cut(s) 27, 2007
PstI CTGCAG 1 cut(s) 170
PsuI RGATCY 2 cut(s) 1394, 2680
PvuII CAGCTG 1 cut(s) 1552
RruI TCGCGA 1 cut(s) 2504
RsaI GTAC 6 cut(s) 431, 833, 1556, 1725, 2337, 2693
RsaNI GTAC 6 cut(s) 430, 832, 1555, 1724, 2336, 2692
RseI CAYNNNNRTG 1 cut(s) 1634
SacI GAGCTC 1 cut(s) 2228
SapI GCTCTTC 2 cut(s) 173, 2570
SatI GCNGC 8 cut(s) 169, 337, 624, 700, 1108, 2493, 2749, 2759
Sau3AI GATC 8 cut(s) 231, 290, 453, 1394, 1518, 1854, 2680, 2733
Sau96I GGNCC 5 cut(s) 27, 87, 978, 1033, 2007
SchI GAGTC 2 cut(s) 1135, 1894
ScrFI CCNGG 4 cut(s) 34, 1315, 2169, 2479
SduI GDGCHC 5 cut(s) 247, 308, 1047, 2160, 2228
SfcI CTRYAG 3 cut(s) 166, 1097, 2073
SinI GGWCC 4 cut(s) 27, 978, 1033, 2007
SmiMI CAYNNNNRTG 1 cut(s) 1634
SmlI CTYRAG 2 cut(s) 601, 2050
SmoI CTYRAG 2 cut(s) 601, 2050
SnaBI TACGTA 1 cut(s) 988
SphI GCATGC 1 cut(s) 706
SsiI CCGC 6 cut(s) 536, 624, 918, 1714, 2061, 2758
SspI AATATT 1 cut(s) 1230
SspMI CTAG 7 cut(s) 14, 577, 607, 1079, 1687, 1954, 2510
SstI GAGCTC 1 cut(s) 2228
StyD4I CCNGG 4 cut(s) 32, 1313, 2167, 2477
StyI CCWWGG 2 cut(s) 1219, 2003
TaiI ACGT 3 cut(s) 990, 2256, 2383
TaqI TCGA 1 cut(s) 2292
TatI WGTACW 4 cut(s) 831, 1554, 1723, 2335
TauI GCSGC 2 cut(s) 626, 2761
TscAI CASTG 6 cut(s) 819, 1707, 2160, 2214, 2424, 2502
TseFI GTSAC 1 cut(s) 948
TseI GCWGC 6 cut(s) 168, 336, 699, 1107, 2492, 2748
Tsp45I GTSAC 1 cut(s) 948
TspGWI ACGGA 1 cut(s) 1984
TspRI CASTG 6 cut(s) 819, 1707, 2160, 2214, 2424, 2502
Vha464I CTTAAG 1 cut(s) 601
VpaK11BI GGWCC 4 cut(s) 27, 978, 1033, 2007
XapI RAATTY 1 cut(s) 136
XceI RCATGY 3 cut(s) 706, 1163, 1245
XmiI GTMKAC 2 cut(s) 377, 1415
XmnI GAANNNNTTC 4 cut(s) 872, 2082, 2413, 2454
XspI CTAG 7 cut(s) 14, 577, 607, 1079, 1687, 1954, 2510
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.