Rroxscaffold_1G00074350

Belongs to the AAA ATPase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0003193
Physical Location & Seq
Reverse (-)
95379618 .. 95384433
4816 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00074350.1

Sequence Viewer

Length: 1452 bp
ATGGCTTTGATTGATGAAAATGCAGGGTGTTTTGAGCAACCATATCATTCCAGTGCATATGAAATGGAGGTGGGGAAAGCTGTGCCAAGCCGGGGCGGTGCTGGCGGGCTGTATCGTATGTATCCGGCTGTTTTGGCTGGTTTGATTGGACTTGGAGGTCTGGAGGTGGCTTATGCAGATGCTGAGGAGGCTGCTTCCAAGCAGGGTTTGCCGGCTGATGCCCCTGCGAATGACGTGGAGGAAATTGCCAAGAAGCAGAGACAGGAAATTGAGGACTTGCTCAAGAGTAAAGGGATCCAACGTGGTTCTTATCCCAACTTTAGTGTTTCTGTCAAGGGGCAAAAGGTAGCGATCAAGTTCCAAATTCCTCCTGCATGCGAAGCTTCACAAGTGATTGCAAATATTGCTTCTAATCTTGGCTTGAAAGTTGAAGAGCATGGTGGTGGTTCAGATATATCATTACGCGCTTGGGACAGTGGAGTTGCTTGGCAACTCATGCTTATGCACCCAGAGAAACAGAAGGAAACTGAAGGTGATCAAGGGGAATTAAAAAATGTGAACGAACATGATCGAGATCTGCACATCCTCGTTTTCCGTTCAGTCATTACCTCAACAGAAAAGGCTAGCTTGATCCACGAGAAAAAATCTACCACAGAACGAAGAGTGAGGGAAGATAGCGCACAAACTTCGTCTATAGAGAAATTAATAACCAGTCTTGAGTCCATGGGAGTGAGAGTTTATGGAGTTGATGAACCCAATATGAGTTCTACAAGCAAAGATATTTCATGGGATAATCTTGCTGGATATGATCTGCAAAAGCGACAGATAGAAGACACGATACTACTGGCTTTGCTTAGTCCTGAAACATATGATGAAATTGCCCGTGGAACTCGTTGCAAATTTGAGTCAAACAGACCTCGAGCTGTGCTCTTCGAAGGCCCACCAGGTACAGGGAAAACTTCTTCTGCTCGTGTCATTGCTAATCAAGCGGCTGTCCTGATGTTATATGTTCCCCTTGAGGTTATCTTGTCCAAGTACTATGGTGAGAGTGAACGATTATTAGGGAAGGTGTTTTCACTTGCTAATGAACTCCCAGATGGTGCTATTATTTTTCTAGATGAGAAGCTTCTTAATAACTTGGCTTCCTATTTGATGCAATTCCAGGTTGATTCGTTTGCTATTTCGCGAGATAGTGATATGCATGAAGCCACACGGAGAGTTTTATCAGTTTTGCTGCGACAGATTGATGGATTTGAACAGGATAAGAAAGTGGTTGTAATTGCTGCAACAAATAGGAAGCAAGACCTCGACCCGGCCTTGATCAGTCGGTTCGATTCAATTGTCCCATTTGGCCTACCTGATCATCAAAATCGTAAGGAAATAGCAGCTCAGTATGCAAAGCATCTAACCGATACTGAATTAGATAAATTGGCAACAGTAACAGAAGAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04141 map04141
Pfam Domains
Protein Families
AAA

Protein Analysis

483

Amino Acids

53.23

Weight (kDa)

5.16

Isoelectric Point (pI)

48.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AAA PF00004 309 - 451 1.7e-27 ATPase family associated with various cellular activities (AAA)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 156
AccII CGCG 2 cut(s) 465, 1186
AciI CCGC 3 cut(s) 96, 105, 989
AclWI GGATC 3 cut(s) 289, 302, 625
AcsI RAATTY 2 cut(s) 363, 899
AcuI CTGAAG 1 cut(s) 549
AfaI GTAC 2 cut(s) 949, 1037
AfiI CCNNNNNNNGG 3 cut(s) 92, 950, 1312
AgsI TTSAA 4 cut(s) 424, 431, 1256, 1338
AjiI CACGTC 1 cut(s) 235
AjnI CCWGG 2 cut(s) 943, 1161
AluBI AGCT 6 cut(s) 80, 383, 627, 923, 1126, 1388
AluI AGCT 6 cut(s) 80, 383, 627, 923, 1126, 1388
Alw21I GWGCWC 1 cut(s) 930
Alw26I GTCTC 1 cut(s) 253
AlwI GGATC 3 cut(s) 289, 302, 625
AlwNI CAGNNNCTG 1 cut(s) 182
Ama87I CYCGRG 1 cut(s) 918
AoxI GGCC 3 cut(s) 937, 1314, 1351
ApeKI GCWGC 4 cut(s) 191, 1234, 1283, 1385
ApoI RAATTY 2 cut(s) 363, 899
AseI ATTAAT 1 cut(s) 704
AspLEI GCGC 2 cut(s) 467, 680
AspS9I GGNCC 1 cut(s) 938
AsuC2I CCSGG 2 cut(s) 92, 1313
AsuHPI GGTGA 2 cut(s) 545, 1055
AsuII TTCGAA 1 cut(s) 933
AsuNHI GCTAGC 1 cut(s) 623
AvaI CYCGRG 1 cut(s) 918
BamHI GGATCC 1 cut(s) 294
BarI GAAGNNNNNNTAC 2 cut(s) 822, 854
BauI CACGAG 2 cut(s) 635, 969
BbsI GAAGAC 1 cut(s) 837
Bbv12I GWGCWC 1 cut(s) 930
BbvCI CCTCAGC 1 cut(s) 183
BbvI GCAGC 4 cut(s) 178, 1221, 1270, 1397
BccI CCATC 2 cut(s) 1091, 1241
BcgI CGANNNNNNTGC 2 cut(s) 669, 703
BciT130I CCWGG 2 cut(s) 945, 1163
BciVI GTATCC 1 cut(s) 132
BclI TGATCA 3 cut(s) 535, 1320, 1360
BcnI CCSGG 2 cut(s) 92, 1313
BcoDI GTCTC 1 cut(s) 253
BfaI CTAG 2 cut(s) 624, 1115
BfmI CTRYAG 1 cut(s) 693
BfuI GTATCC 1 cut(s) 132
BglII AGATCT 1 cut(s) 574
BisI GCNGC 5 cut(s) 192, 990, 1235, 1284, 1386
BlsI GCNGC 5 cut(s) 193, 991, 1236, 1285, 1387
BmcAI AGTACT 1 cut(s) 1037
Bme1390I CCNGG 4 cut(s) 92, 945, 1163, 1313
BmeT110I CYCGRG 1 cut(s) 918
BmgBI CACGTC 1 cut(s) 235
BmgT120I GGNCC 1 cut(s) 938
BmiI GGNNCC 1 cut(s) 296
BmrFI CCNGG 4 cut(s) 92, 945, 1163, 1313
BmsI GCATC 4 cut(s) 169, 208, 1143, 1411
BmtI GCTAGC 1 cut(s) 627
BpiI GAAGAC 1 cut(s) 837
BplI GAGNNNNNCTC 2 cut(s) 912, 944
BpmI CTGGAG 1 cut(s) 182
Bpu10I CCTNAGC 1 cut(s) 183
Bpu14I TTCGAA 1 cut(s) 933
BpuEI CTTGAG 3 cut(s) 266, 737, 1037
BpuMI CCSGG 2 cut(s) 92, 1313
BsaBI GATNNNNATC 1 cut(s) 573
BsaJI CCNNGG 3 cut(s) 91, 723, 883
BsaXI ACNNNNNCTCC 2 cut(s) 720, 750
Bsc4I CCNNNNNNNGG 3 cut(s) 92, 950, 1312
Bse118I RCCGGY 1 cut(s) 211
Bse1I ACTGG 3 cut(s) 51, 711, 849
Bse3DI GCAATG 1 cut(s) 975
Bse8I GATNNNNATC 1 cut(s) 573
BseBI CCWGG 2 cut(s) 945, 1163
BseDI CCNNGG 3 cut(s) 91, 723, 883
BseGI GGATG 1 cut(s) 582
BseJI GATNNNNATC 1 cut(s) 573
BseLI CCNNNNNNNGG 3 cut(s) 92, 950, 1312
BseMI GCAATG 1 cut(s) 975
BseMII CTCAG 2 cut(s) 174, 1403
BseNI ACTGG 3 cut(s) 51, 711, 849
BseRI GAGGAG 1 cut(s) 200
BseXI GCAGC 4 cut(s) 178, 1221, 1270, 1397
BsgI GTGCAG 1 cut(s) 563
Bsh1236I CGCG 2 cut(s) 465, 1186
BshFI GGCC 3 cut(s) 939, 1316, 1353
BsiHKAI GWGCWC 1 cut(s) 930
BsiHKCI CYCGRG 1 cut(s) 918
BsiSI CCGG 4 cut(s) 91, 125, 212, 1313
BslFI GGGAC 2 cut(s) 485, 1328
BslI CCNNNNNNNGG 3 cut(s) 92, 950, 1312
BsmAI GTCTC 1 cut(s) 253
BsmFI GGGAC 2 cut(s) 485, 1328
BsnI GGCC 3 cut(s) 939, 1316, 1353
BsoBI CYCGRG 1 cut(s) 918
Bsp119I TTCGAA 1 cut(s) 933
Bsp1286I GDGCHC 1 cut(s) 930
Bsp143I GATC 9 cut(s) 294, 351, 535, 568, 574, 630, 808, 1320, 1360
Bsp19I CCATGG 1 cut(s) 723
Bsp68I TCGCGA 1 cut(s) 1186
BspACI CCGC 3 cut(s) 96, 105, 989
BspANI GGCC 3 cut(s) 939, 1316, 1353
BspCNI CTCAG 2 cut(s) 175, 1402
BspFNI CGCG 2 cut(s) 465, 1186
BspLI GGNNCC 1 cut(s) 296
BspOI GCTAGC 1 cut(s) 627
BspPI GGATC 3 cut(s) 289, 302, 625
BspQI GCTCTTC 2 cut(s) 426, 935
BspT104I TTCGAA 1 cut(s) 933
BsrDI GCAATG 1 cut(s) 975
BsrFI RCCGGY 1 cut(s) 211
BsrI ACTGG 3 cut(s) 51, 711, 849
BssAI RCCGGY 1 cut(s) 211
BssECI CCNNGG 3 cut(s) 91, 723, 883
BssMI GATC 9 cut(s) 294, 351, 535, 568, 574, 630, 808, 1320, 1360
BssSI CACGAG 2 cut(s) 635, 969
BssT1I CCWWGG 1 cut(s) 723
Bst2BI CACGAG 2 cut(s) 635, 969
Bst2UI CCWGG 2 cut(s) 945, 1163
Bst4CI ACNGT 2 cut(s) 476, 1438
Bst6I CTCTTC 4 cut(s) 426, 655, 935, 1440
BstAPI GCANNNNNTGC 3 cut(s) 208, 404, 496
BstBI TTCGAA 1 cut(s) 933
BstC8I GCNNGC 5 cut(s) 103, 107, 213, 376, 625
BstDEI CTNAG 3 cut(s) 183, 854, 1389
BstDSI CCRYGG 2 cut(s) 723, 883
BstF5I GGATG 1 cut(s) 582
BstFNI CGCG 2 cut(s) 465, 1186
BstHHI GCGC 2 cut(s) 467, 680
BstKTI GATC 9 cut(s) 297, 354, 538, 571, 577, 633, 811, 1323, 1363
BstMAI GTCTC 1 cut(s) 253
BstMBI GATC 9 cut(s) 294, 351, 535, 568, 574, 630, 808, 1320, 1360
BstMWI GCNNNNNNNGC 9 cut(s) 102, 134, 188, 208, 380, 404, 496, 986, 1394
BstNI CCWGG 2 cut(s) 945, 1163
BstNSI RCATGY 1 cut(s) 378
BstSCI CCNGG 4 cut(s) 90, 943, 1161, 1311
BstSFI CTRYAG 1 cut(s) 693
BstUI CGCG 2 cut(s) 465, 1186
BstV1I GCAGC 4 cut(s) 178, 1221, 1270, 1397
BstV2I GAAGAC 1 cut(s) 837
BstX2I RGATCY 2 cut(s) 294, 574
BstYI RGATCY 2 cut(s) 294, 574
BsuI GTATCC 1 cut(s) 132
BsuRI GGCC 3 cut(s) 939, 1316, 1353
BtgI CCRYGG 2 cut(s) 723, 883
BtrI CACGTC 1 cut(s) 235
BtsCI GGATG 1 cut(s) 582
BtsIMutI CAGTG 2 cut(s) 58, 481
BtuMI TCGCGA 1 cut(s) 1186
Cac8I GCNNGC 5 cut(s) 103, 107, 213, 376, 625
CaiI CAGNNNCTG 1 cut(s) 182
CfoI GCGC 2 cut(s) 467, 680
Cfr10I RCCGGY 1 cut(s) 211
Cfr13I GGNCC 1 cut(s) 938
CsiI ACCWGGT 1 cut(s) 943
Csp6I GTAC 2 cut(s) 948, 1036
CviAII CATG 7 cut(s) 375, 437, 496, 566, 724, 786, 1202
CviQI GTAC 2 cut(s) 948, 1036
DdeI CTNAG 3 cut(s) 183, 854, 1389
DpnI GATC 9 cut(s) 296, 353, 537, 570, 576, 632, 810, 1322, 1362
DpnII GATC 9 cut(s) 294, 351, 535, 568, 574, 630, 808, 1320, 1360
DrdI GACNNNNNNGTC 1 cut(s) 156
DseDI GACNNNNNNGTC 1 cut(s) 156
Eam1104I CTCTTC 4 cut(s) 426, 655, 935, 1440
EarI CTCTTC 4 cut(s) 426, 655, 935, 1440
Eco130I CCWWGG 1 cut(s) 723
Eco57I CTGAAG 1 cut(s) 549
Eco88I CYCGRG 1 cut(s) 918
EcoRII CCWGG 2 cut(s) 943, 1161
EcoT14I CCWWGG 1 cut(s) 723
EcoT22I ATGCAT 1 cut(s) 1203
ErhI CCWWGG 1 cut(s) 723
FaeI CATG 7 cut(s) 378, 440, 499, 569, 727, 789, 1205
FalI AAGNNNNNCTT 2 cut(s) 611, 643
FaqI GGGAC 2 cut(s) 485, 1328
FatI CATG 7 cut(s) 374, 436, 495, 565, 723, 785, 1201
FauI CCCGC 1 cut(s) 98
FauNDI CATATG 2 cut(s) 58, 868
FbaI TGATCA 3 cut(s) 535, 1320, 1360
Fnu4HI GCNGC 5 cut(s) 192, 990, 1235, 1284, 1386
FokI GGATG 1 cut(s) 569
Fsp4HI GCNGC 5 cut(s) 192, 990, 1235, 1284, 1386
FspBI CTAG 2 cut(s) 624, 1115
GlaI GCGC 2 cut(s) 466, 679
GluI GCNGC 5 cut(s) 192, 990, 1235, 1284, 1386
GsuI CTGGAG 1 cut(s) 182
HaeIII GGCC 3 cut(s) 939, 1316, 1353
HapII CCGG 4 cut(s) 91, 125, 212, 1313
HhaI GCGC 2 cut(s) 467, 680
Hin1II CATG 7 cut(s) 378, 440, 499, 569, 727, 789, 1205
Hin6I GCGC 2 cut(s) 465, 678
HinP1I GCGC 2 cut(s) 465, 678
HindIII AAGCTT 2 cut(s) 381, 1124
HinfI GANTC 4 cut(s) 719, 905, 1169, 1334
HpaII CCGG 4 cut(s) 91, 125, 212, 1313
HphI GGTGA 2 cut(s) 545, 1055
Hpy166II GTNNAC 2 cut(s) 559, 1052
Hpy188I TCNGA 1 cut(s) 451
Hpy188III TCNNGA 8 cut(s) 161, 283, 572, 716, 860, 997, 1115, 1185
Hpy8I GTNNAC 2 cut(s) 559, 1052
HpyAV CCTTC 4 cut(s) 514, 524, 929, 1060
HpyCH4III ACNGT 2 cut(s) 476, 1438
HpyCH4IV ACGT 2 cut(s) 234, 301
HpyF10VI GCNNNNNNNGC 9 cut(s) 102, 134, 188, 208, 380, 404, 496, 986, 1394
HpyF3I CTNAG 3 cut(s) 183, 854, 1389
HpySE526I ACGT 2 cut(s) 234, 301
Hsp92II CATG 7 cut(s) 378, 440, 499, 569, 727, 789, 1205
HspAI GCGC 2 cut(s) 465, 678
KroI GCCGGC 1 cut(s) 211
KroNI GCCGGC 1 cut(s) 213
Ksp22I TGATCA 3 cut(s) 535, 1320, 1360
Kzo9I GATC 9 cut(s) 294, 351, 535, 568, 574, 630, 808, 1320, 1360
LguI GCTCTTC 2 cut(s) 426, 935
Lsp1109I GCAGC 4 cut(s) 178, 1221, 1270, 1397
LweI GCATC 4 cut(s) 169, 208, 1143, 1411
MabI ACCWGGT 1 cut(s) 943
MaeI CTAG 2 cut(s) 624, 1115
MaeII ACGT 2 cut(s) 234, 301
MaeIII GTNAC 1 cut(s) 1438
MalI GATC 9 cut(s) 296, 353, 537, 570, 576, 632, 810, 1322, 1362
MboI GATC 9 cut(s) 294, 351, 535, 568, 574, 630, 808, 1320, 1360
MboII GAAGA 6 cut(s) 443, 672, 683, 842, 922, 954
MfeI CAATTG 1 cut(s) 1338
MflI RGATCY 2 cut(s) 294, 574
MhlI GDGCHC 1 cut(s) 930
MlyI GAGTC 2 cut(s) 728, 914
MmeI TCCRAC 1 cut(s) 322
Mph1103I ATGCAT 1 cut(s) 1203
MroNI GCCGGC 1 cut(s) 211
MseI TTAA 3 cut(s) 548, 704, 1131
MslI CAYNNNNRTG 4 cut(s) 51, 441, 500, 728
MspI CCGG 4 cut(s) 91, 125, 212, 1313
MspR9I CCNGG 4 cut(s) 92, 945, 1163, 1313
MunI CAATTG 1 cut(s) 1338
MvaI CCWGG 2 cut(s) 945, 1163
MvnI CGCG 2 cut(s) 465, 1186
MwoI GCNNNNNNNGC 9 cut(s) 102, 134, 188, 208, 380, 404, 496, 986, 1394
NaeI GCCGGC 1 cut(s) 213
NciI CCSGG 2 cut(s) 92, 1313
NcoI CCATGG 1 cut(s) 723
NdeI CATATG 2 cut(s) 58, 868
NdeII GATC 9 cut(s) 294, 351, 535, 568, 574, 630, 808, 1320, 1360
NgoMIV GCCGGC 1 cut(s) 211
NheI GCTAGC 1 cut(s) 623
NlaIII CATG 7 cut(s) 378, 440, 499, 569, 727, 789, 1205
NlaIV GGNNCC 1 cut(s) 296
NruI TCGCGA 1 cut(s) 1186
NsiI ATGCAT 1 cut(s) 1203
NspI RCATGY 1 cut(s) 378
NspV TTCGAA 1 cut(s) 933
PaeI GCATGC 1 cut(s) 378
PaeR7I CTCGAG 1 cut(s) 918
PciSI GCTCTTC 2 cut(s) 426, 935
PdiI GCCGGC 1 cut(s) 213
PfeI GAWTC 2 cut(s) 1169, 1334
PkrI GCNGC 5 cut(s) 193, 991, 1236, 1285, 1387
PleI GAGTC 2 cut(s) 727, 913
PpsI GAGTC 2 cut(s) 727, 913
PshBI ATTAAT 1 cut(s) 704
Psp6I CCWGG 2 cut(s) 943, 1161
PspGI CCWGG 2 cut(s) 943, 1161
PspN4I GGNNCC 1 cut(s) 296
PspPI GGNCC 1 cut(s) 938
PspXI VCTCGAGB 1 cut(s) 918
PstNI CAGNNNCTG 1 cut(s) 182
PsuI RGATCY 2 cut(s) 294, 574
RruI TCGCGA 1 cut(s) 1186
RsaI GTAC 2 cut(s) 949, 1037
RsaNI GTAC 2 cut(s) 948, 1036
RseI CAYNNNNRTG 4 cut(s) 51, 441, 500, 728
SapI GCTCTTC 2 cut(s) 426, 935
SaqAI TTAA 3 cut(s) 548, 704, 1131
SatI GCNGC 5 cut(s) 192, 990, 1235, 1284, 1386
Sau3AI GATC 9 cut(s) 294, 351, 535, 568, 574, 630, 808, 1320, 1360
Sau96I GGNCC 1 cut(s) 938
ScaI AGTACT 1 cut(s) 1037
SchI GAGTC 2 cut(s) 728, 914
ScrFI CCNGG 4 cut(s) 92, 945, 1163, 1313
SduI GDGCHC 1 cut(s) 930
SexAI ACCWGGT 1 cut(s) 943
SfaNI GCATC 4 cut(s) 169, 208, 1143, 1411
SfcI CTRYAG 1 cut(s) 693
Sfr274I CTCGAG 1 cut(s) 918
SfuI TTCGAA 1 cut(s) 933
SlaI CTCGAG 1 cut(s) 918
SmiMI CAYNNNNRTG 4 cut(s) 51, 441, 500, 728
SmlI CTYRAG 4 cut(s) 281, 716, 918, 1016
SmoI CTYRAG 4 cut(s) 281, 716, 918, 1016
SphI GCATGC 1 cut(s) 378
SsiI CCGC 3 cut(s) 96, 105, 989
SspI AATATT 1 cut(s) 403
SspMI CTAG 2 cut(s) 624, 1115
StyD4I CCNGG 4 cut(s) 90, 943, 1161, 1311
StyI CCWWGG 1 cut(s) 723
TaaI ACNGT 2 cut(s) 476, 1438
TaiI ACGT 2 cut(s) 237, 304
TaqI TCGA 5 cut(s) 571, 919, 933, 1308, 1332
TatI WGTACW 1 cut(s) 1035
TauI GCSGC 1 cut(s) 992
TfiI GAWTC 2 cut(s) 1169, 1334
Tru1I TTAA 3 cut(s) 548, 704, 1131
Tru9I TTAA 3 cut(s) 548, 704, 1131
TscAI CASTG 2 cut(s) 58, 481
TseI GCWGC 4 cut(s) 191, 1234, 1283, 1385
TspDTI ATGAA 7 cut(s) 30, 75, 765, 774, 888, 1101, 1218
TspGWI ACGGA 2 cut(s) 584, 1228
TspRI CASTG 2 cut(s) 58, 481
VspI ATTAAT 1 cut(s) 704
XapI RAATTY 2 cut(s) 363, 899
XbaI TCTAGA 1 cut(s) 1114
XceI RCATGY 1 cut(s) 378
XhoI CTCGAG 1 cut(s) 918
XspI CTAG 2 cut(s) 624, 1115
ZrmI AGTACT 1 cut(s) 1037
Zsp2I ATGCAT 1 cut(s) 1203
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.