Rorug04G0394300

Belongs to the AAA ATPase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0003193
Physical Location & Seq
Forward (+)
56055802 .. 56059400
3599 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0394300.1

Sequence Viewer

Length: 1296 bp
ATGAGCTCCTCCTCCGACTCGTCGGGAGCTAAGCCGTGCGCGGCGACGGCCGACAAGGACAAGCAGAAGGAGAAGGCGAGAGTGAGCCGCACCTCCCTCATACTCTGGCACGCTCACCACAACGACGCCGTCGCAGTCCGCAAGCTTCTCGAGGAGGATCGCGCCCTCGTTCACGCCAGAGACTACGACAACCGCACTCCGCTCCACGTCGCCTCCCTCCACGGCTGGATCGACGTCGCCAAATGCCTCATCGAGTACGGCGCCGATGTCAACGCCCAGGATCGTTGGAAGAACACTCCTCTAGCTGATGCCGAAGGAGCTAAGAAGCACACCATGATTGAGCTGTTGAAATCTTATGGTGGCTTGTCTTATGGCCAAAATGGGAGCCATTTCGAACCGAAGCCGGTGGCGCCTCCTCTGCCAAACAAGTGTGATTGGGAAATTGAGCCTGCTGAGCTTGACTTCTCCGGATCAGTTACCATCGGGAAGGGCTCGTTTGGTGAGATTTTAAAAGCACATTGGCGTGGAACACCAGTAGCTGTCAAACGCATTCTTCCATCTCTTTCTGGTGATAGATTAGTGATTCAGGACTTCCGGCATGAAGTAAATTTGCTAGTGAAGCTTCGCCACCCTAATATAGTCCAATTTCTTGGAGCTGTCACAGAGAAGAAGCCTCTTATGTTAATTACGGAGTATTTAAGAGGGGGAGATCTTCATCAGTACCTTAAGGAAAAGGGTTCACTTAGTCCTACAACAGCTGTCAGCTTTGCCTTGGACATTGCAAGAGGGATGGCTTATCTTCACAATGAGCCAAATGTTATAATTCATCGTGACCTAAAACCAAGGAATGTTCTTTTAGTCAATTCCAGTGCAGACCATCTCAAAGTTGGAGATTTTGGACTAAGCAAGCTCATCAAGGTTCAAAATTCTCATGATGTGTACAAAATGACTGGCGAAACTGGAAGTTACCGATATATGGCTCCCGAAGTTTTTAAGCACCGGCGATATGATAAGAAGGTTGATGTGTTCTCTTTTGCAATGATACTGTATGAGATGCTCGAAGGAGATCCACCACTTTCAAATTATGAGCCTTATGATGCAGCCAAATTTGTGGCAGAAGGACACAGGCCAATGTTTCATGGAAAAGGACACACCCCTGAACTGAAAGAGTTAATAGAGCAATGCTGGTCTGCTGACATGAACCAAAGACCTTCTTTCTTGGAAATTCTGAAAAAGCTTGAGAAGGTAAAGGAAAATCTTCAAACAGATCATCACTGGCACATATTCAATACATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04141 map04141
Pfam Domains
Protein Families
AAA

Protein Analysis

431

Amino Acids

48.66

Weight (kDa)

8.16

Isoelectric Point (pI)

36.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ank_2 PF12796 24 - 94 1.1e-08 Ankyrin repeats (3 copies)
Ank_2 PF12796 37 - 119 1.7e-08 Ankyrin repeats (3 copies)
Ank_5 PF13857 49 - 101 5.3e-06 Ankyrin repeats (many copies)
Ank PF00023 63 - 95 9.8e-08 Ankyrin repeat
Ank_3 PF13606 63 - 92 4.1e-06 Ankyrin repeat
Pkinase PF00069 159 - 411 1.1e-57 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 160 - 413 1e-64 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 821
AatII GACGTC 1 cut(s) 237
AccB1I GGYRCC 2 cut(s) 260, 409
AccBSI CCGCTC 1 cut(s) 202
AccII CGCG 2 cut(s) 41, 162
AccIII TCCGGA 1 cut(s) 467
AciI CCGC 5 cut(s) 41, 88, 139, 193, 200
AclWI GGATC 5 cut(s) 165, 236, 288, 478, 1061
AcoI YGGCCR 2 cut(s) 48, 373
AcsI RAATTY 4 cut(s) 607, 925, 1106, 1224
AcyI GRCGYC 4 cut(s) 126, 234, 261, 410
AfaI GTAC 3 cut(s) 257, 722, 941
AfiI CCNNNNNNNGG 1 cut(s) 976
AflII CTTAAG 1 cut(s) 725
AgsI TTSAA 5 cut(s) 349, 923, 1080, 1262, 1288
AjiI CACGTC 1 cut(s) 208
AjnI CCWGG 1 cut(s) 276
Alw21I GWGCWC 1 cut(s) 8
Alw26I GTCTC 1 cut(s) 174
AlwI GGATC 5 cut(s) 165, 236, 288, 478, 1061
AlwNI CAGNNNCTG 1 cut(s) 539
Ama87I CYCGRG 1 cut(s) 149
Aor13HI TCCGGA 1 cut(s) 467
AoxI GGCC 3 cut(s) 48, 373, 1127
ApeKI GCWGC 1 cut(s) 1100
ApoI RAATTY 4 cut(s) 607, 925, 1106, 1224
Asp700I GAANNNNTTC 1 cut(s) 1257
AspLEI GCGC 4 cut(s) 41, 164, 263, 412
AsuHPI GGTGA 3 cut(s) 107, 512, 581
AsuII TTCGAA 1 cut(s) 393
AvaI CYCGRG 1 cut(s) 149
BalI TGGCCA 1 cut(s) 375
BanI GGYRCC 2 cut(s) 260, 409
BanII GRGCYC 2 cut(s) 8, 494
Bbv12I GWGCWC 1 cut(s) 8
BbvI GCAGC 1 cut(s) 1112
BccI CCATC 4 cut(s) 488, 565, 784, 885
BceAI ACGGC 5 cut(s) 19, 63, 113, 238, 274
BcgI CGANNNNNNTGC 2 cut(s) 130, 164
BciT130I CCWGG 1 cut(s) 278
BcoDI GTCTC 1 cut(s) 174
BfaI CTAG 2 cut(s) 302, 614
BfoI RGCGCY 2 cut(s) 264, 413
BfrI CTTAAG 1 cut(s) 725
BglII AGATCT 1 cut(s) 709
BisI GCNGC 3 cut(s) 42, 88, 1101
BlpI GCTNAGC 2 cut(s) 30, 453
BlsI GCNGC 3 cut(s) 43, 89, 1102
Bme1390I CCNGG 1 cut(s) 278
BmeT110I CYCGRG 1 cut(s) 149
BmgBI CACGTC 1 cut(s) 208
BmiI GGNNCC 4 cut(s) 262, 386, 411, 981
BmrFI CCNGG 1 cut(s) 278
BmsI GCATC 3 cut(s) 298, 1044, 1087
Bpu1102I GCTNAGC 2 cut(s) 30, 453
Bpu14I TTCGAA 1 cut(s) 393
BpuEI CTTGAG 1 cut(s) 1259
BsaBI GATNNNNATC 1 cut(s) 714
BsaHI GRCGYC 4 cut(s) 126, 234, 261, 410
BsaJI CCNNGG 4 cut(s) 220, 276, 771, 842
BsaWI WCCGGW 1 cut(s) 467
BsaXI ACNNNNNCTCC 2 cut(s) 197, 227
Bsc4I CCNNNNNNNGG 1 cut(s) 976
Bse118I RCCGGY 2 cut(s) 403, 999
Bse1I ACTGG 5 cut(s) 533, 867, 955, 964, 1280
Bse3DI GCAATG 3 cut(s) 777, 1044, 1187
Bse8I GATNNNNATC 1 cut(s) 714
BseAI TCCGGA 1 cut(s) 467
BseBI CCWGG 1 cut(s) 278
BseDI CCNNGG 4 cut(s) 220, 276, 771, 842
BseGI GGATG 1 cut(s) 795
BseJI GATNNNNATC 1 cut(s) 714
BseLI CCNNNNNNNGG 1 cut(s) 976
BseMI GCAATG 3 cut(s) 777, 1044, 1187
BseMII CTCAG 1 cut(s) 444
BseNI ACTGG 5 cut(s) 533, 867, 955, 964, 1280
BseRI GAGGAG 3 cut(s) 167, 288, 405
BseX3I CGGCCG 1 cut(s) 48
BseXI GCAGC 1 cut(s) 1112
BsgI GTGCAG 1 cut(s) 891
Bsh1236I CGCG 2 cut(s) 41, 162
Bsh1285I CGRYCG 1 cut(s) 51
BshFI GGCC 3 cut(s) 50, 375, 1129
BshNI GGYRCC 2 cut(s) 260, 409
BsiEI CGRYCG 1 cut(s) 51
BsiHKAI GWGCWC 1 cut(s) 8
BsiHKCI CYCGRG 1 cut(s) 149
BsiSI CCGG 4 cut(s) 404, 468, 595, 1000
BslI CCNNNNNNNGG 1 cut(s) 976
BsmAI GTCTC 1 cut(s) 174
BsmI GAATGC 1 cut(s) 549
BsnI GGCC 3 cut(s) 50, 375, 1129
BsoBI CYCGRG 1 cut(s) 149
Bsp119I TTCGAA 1 cut(s) 393
Bsp1286I GDGCHC 2 cut(s) 8, 494
Bsp13I TCCGGA 1 cut(s) 467
Bsp1407I TGTACA 1 cut(s) 939
Bsp143I GATC 7 cut(s) 157, 228, 280, 470, 709, 1066, 1267
Bsp1720I GCTNAGC 2 cut(s) 30, 453
BspACI CCGC 5 cut(s) 41, 88, 139, 193, 200
BspANI GGCC 3 cut(s) 50, 375, 1129
BspCNI CTCAG 1 cut(s) 445
BspEI TCCGGA 1 cut(s) 467
BspFNI CGCG 2 cut(s) 41, 162
BspHI TCATGA 1 cut(s) 931
BspLI GGNNCC 4 cut(s) 262, 386, 411, 981
BspPI GGATC 5 cut(s) 165, 236, 288, 478, 1061
BspT104I TTCGAA 1 cut(s) 393
BspT107I GGYRCC 2 cut(s) 260, 409
BspTI CTTAAG 1 cut(s) 725
BsrBI CCGCTC 1 cut(s) 202
BsrDI GCAATG 3 cut(s) 777, 1044, 1187
BsrFI RCCGGY 2 cut(s) 403, 999
BsrGI TGTACA 1 cut(s) 939
BsrI ACTGG 5 cut(s) 533, 867, 955, 964, 1280
BssAI RCCGGY 2 cut(s) 403, 999
BssECI CCNNGG 4 cut(s) 220, 276, 771, 842
BssMI GATC 7 cut(s) 157, 228, 280, 470, 709, 1066, 1267
BssNI GRCGYC 4 cut(s) 126, 234, 261, 410
BssT1I CCWWGG 2 cut(s) 771, 842
Bst2UI CCWGG 1 cut(s) 278
Bst4CI ACNGT 1 cut(s) 1047
BstACI GRCGYC 4 cut(s) 126, 234, 261, 410
BstAFI CTTAAG 1 cut(s) 725
BstAUI TGTACA 1 cut(s) 939
BstBI TTCGAA 1 cut(s) 393
BstC8I GCNNGC 4 cut(s) 111, 143, 450, 908
BstDEI CTNAG 5 cut(s) 30, 321, 453, 743, 902
BstDSI CCRYGG 1 cut(s) 220
BstF5I GGATG 1 cut(s) 795
BstFNI CGCG 2 cut(s) 41, 162
BstH2I RGCGCY 2 cut(s) 264, 413
BstHHI GCGC 4 cut(s) 41, 164, 263, 412
BstKTI GATC 7 cut(s) 160, 231, 283, 473, 712, 1069, 1270
BstMAI GTCTC 1 cut(s) 174
BstMBI GATC 7 cut(s) 157, 228, 280, 470, 709, 1066, 1267
BstMCI CGRYCG 1 cut(s) 51
BstMWI GCNNNNNNNGC 6 cut(s) 47, 317, 409, 418, 454, 619
BstNI CCWGG 1 cut(s) 278
BstSCI CCNGG 1 cut(s) 276
BstUI CGCG 2 cut(s) 41, 162
BstV1I GCAGC 1 cut(s) 1112
BstX2I RGATCY 2 cut(s) 709, 1066
BstXI CCANNNNNNTGG 2 cut(s) 650, 1111
BstYI RGATCY 2 cut(s) 709, 1066
BstZI CGGCCG 1 cut(s) 48
BsuRI GGCC 3 cut(s) 50, 375, 1129
BtgI CCRYGG 1 cut(s) 220
BtrI CACGTC 1 cut(s) 208
BtsCI GGATG 1 cut(s) 795
BtsIMutI CAGTG 2 cut(s) 874, 1273
Cac8I GCNNGC 4 cut(s) 111, 143, 450, 908
CaiI CAGNNNCTG 1 cut(s) 539
CciI TCATGA 1 cut(s) 931
CfoI GCGC 4 cut(s) 41, 164, 263, 412
Cfr10I RCCGGY 2 cut(s) 403, 999
CseI GACGC 1 cut(s) 134
Csp6I GTAC 3 cut(s) 256, 721, 940
CviAII CATG 5 cut(s) 334, 599, 932, 1139, 1198
CviQI GTAC 3 cut(s) 256, 721, 940
DdeI CTNAG 5 cut(s) 30, 321, 453, 743, 902
DinI GGCGCC 2 cut(s) 262, 411
DpnI GATC 7 cut(s) 159, 230, 282, 472, 711, 1068, 1269
DpnII GATC 7 cut(s) 157, 228, 280, 470, 709, 1066, 1267
DraI TTTAAA 1 cut(s) 510
EaeI YGGCCR 2 cut(s) 48, 373
EagI CGGCCG 1 cut(s) 48
Ecl136II GAGCTC 1 cut(s) 6
EclXI CGGCCG 1 cut(s) 48
Eco130I CCWWGG 2 cut(s) 771, 842
Eco24I GRGCYC 2 cut(s) 8, 494
Eco52I CGGCCG 1 cut(s) 48
Eco53kI GAGCTC 1 cut(s) 6
Eco88I CYCGRG 1 cut(s) 149
EcoICRI GAGCTC 1 cut(s) 6
EcoRII CCWGG 1 cut(s) 276
EcoT14I CCWWGG 2 cut(s) 771, 842
EcoT38I GRGCYC 2 cut(s) 8, 494
EgeI GGCGCC 2 cut(s) 262, 411
EheI GGCGCC 2 cut(s) 262, 411
ErhI CCWWGG 2 cut(s) 771, 842
FaeI CATG 5 cut(s) 337, 602, 935, 1142, 1201
FalI AAGNNNNNCTT 2 cut(s) 1198, 1230
FatI CATG 5 cut(s) 333, 598, 931, 1138, 1197
Fnu4HI GCNGC 3 cut(s) 42, 88, 1101
FokI GGATG 1 cut(s) 802
FriOI GRGCYC 2 cut(s) 8, 494
Fsp4HI GCNGC 3 cut(s) 42, 88, 1101
FspBI CTAG 2 cut(s) 302, 614
GlaI GCGC 4 cut(s) 40, 163, 262, 411
GluI GCNGC 3 cut(s) 42, 88, 1101
HaeII RGCGCY 2 cut(s) 264, 413
HaeIII GGCC 3 cut(s) 50, 375, 1129
HapII CCGG 4 cut(s) 404, 468, 595, 1000
HgaI GACGC 1 cut(s) 134
HhaI GCGC 4 cut(s) 41, 164, 263, 412
Hin1I GRCGYC 4 cut(s) 126, 234, 261, 410
Hin1II CATG 5 cut(s) 337, 602, 935, 1142, 1201
Hin6I GCGC 4 cut(s) 39, 162, 261, 410
HinP1I GCGC 4 cut(s) 39, 162, 261, 410
HincII GTYRAC 1 cut(s) 271
HindII GTYRAC 1 cut(s) 271
HindIII AAGCTT 3 cut(s) 143, 620, 1235
HinfI GANTC 2 cut(s) 17, 583
HpaII CCGG 4 cut(s) 404, 468, 595, 1000
HphI GGTGA 3 cut(s) 107, 512, 581
Hpy166II GTNNAC 4 cut(s) 172, 271, 740, 940
Hpy188I TCNGA 2 cut(s) 16, 1230
Hpy188III TCNNGA 8 cut(s) 24, 149, 468, 484, 587, 830, 932, 983
Hpy8I GTNNAC 4 cut(s) 172, 271, 740, 940
Hpy99I CGWCG 7 cut(s) 25, 49, 128, 134, 212, 236, 239
HpyAV CCTTC 9 cut(s) 61, 67, 308, 481, 1009, 1055, 1112, 1221, 1237
HpyCH4III ACNGT 1 cut(s) 1047
HpyCH4IV ACGT 2 cut(s) 207, 234
HpyCH4V TGCA 4 cut(s) 782, 872, 1037, 1100
HpyF10VI GCNNNNNNNGC 6 cut(s) 47, 317, 409, 418, 454, 619
HpyF3I CTNAG 5 cut(s) 30, 321, 453, 743, 902
HpySE526I ACGT 2 cut(s) 207, 234
Hsp92I GRCGYC 4 cut(s) 126, 234, 261, 410
Hsp92II CATG 5 cut(s) 337, 602, 935, 1142, 1201
HspAI GCGC 4 cut(s) 39, 162, 261, 410
KasI GGCGCC 2 cut(s) 260, 409
Kpn2I TCCGGA 1 cut(s) 467
Kzo9I GATC 7 cut(s) 157, 228, 280, 470, 709, 1066, 1267
LmnI GCTCC 7 cut(s) 11, 26, 207, 317, 384, 653, 985
Lsp1109I GCAGC 1 cut(s) 1112
LweI GCATC 3 cut(s) 298, 1044, 1087
MaeI CTAG 2 cut(s) 302, 614
MaeII ACGT 2 cut(s) 207, 234
MaeIII GTNAC 4 cut(s) 475, 658, 830, 965
MalI GATC 7 cut(s) 159, 230, 282, 472, 711, 1068, 1269
MbiI CCGCTC 1 cut(s) 202
MboI GATC 7 cut(s) 157, 228, 280, 470, 709, 1066, 1267
MboII GAAGA 6 cut(s) 301, 545, 679, 704, 791, 1250
MflI RGATCY 2 cut(s) 709, 1066
MhlI GDGCHC 2 cut(s) 8, 494
MlsI TGGCCA 1 cut(s) 375
MluNI TGGCCA 1 cut(s) 375
Mly113I GGCGCC 2 cut(s) 261, 410
MlyI GAGTC 1 cut(s) 11
MmeI TCCRAC 3 cut(s) 39, 266, 868
Mox20I TGGCCA 1 cut(s) 375
MroI TCCGGA 1 cut(s) 467
MroXI GAANNNNTTC 1 cut(s) 1257
MscI TGGCCA 1 cut(s) 375
MseI TTAA 6 cut(s) 509, 683, 698, 726, 993, 1172
MslI CAYNNNNRTG 1 cut(s) 522
Msp20I TGGCCA 1 cut(s) 375
MspA1I CMGCKG 1 cut(s) 758
MspCI CTTAAG 1 cut(s) 725
MspI CCGG 4 cut(s) 404, 468, 595, 1000
MspR9I CCNGG 1 cut(s) 278
Mva1269I GAATGC 1 cut(s) 549
MvaI CCWGG 1 cut(s) 278
MvnI CGCG 2 cut(s) 41, 162
MwoI GCNNNNNNNGC 6 cut(s) 47, 317, 409, 418, 454, 619
NarI GGCGCC 2 cut(s) 261, 410
NdeII GATC 7 cut(s) 157, 228, 280, 470, 709, 1066, 1267
NlaIII CATG 5 cut(s) 337, 602, 935, 1142, 1201
NlaIV GGNNCC 4 cut(s) 262, 386, 411, 981
NmuCI GTSAC 2 cut(s) 658, 830
NspV TTCGAA 1 cut(s) 393
PaeR7I CTCGAG 1 cut(s) 149
PagI TCATGA 1 cut(s) 931
PcsI WCGNNNNNNNCGW 1 cut(s) 228
PctI GAATGC 1 cut(s) 549
PdmI GAANNNNTTC 1 cut(s) 1257
PfeI GAWTC 1 cut(s) 583
PflFI GACNNNGTC 1 cut(s) 128
PkrI GCNGC 3 cut(s) 43, 89, 1102
PleI GAGTC 1 cut(s) 11
PluTI GGCGCC 2 cut(s) 264, 413
PpsI GAGTC 1 cut(s) 11
PsiI TTATAA 1 cut(s) 821
Psp124BI GAGCTC 1 cut(s) 8
Psp6I CCWGG 1 cut(s) 276
PspGI CCWGG 1 cut(s) 276
PspN4I GGNNCC 4 cut(s) 262, 386, 411, 981
PstNI CAGNNNCTG 1 cut(s) 539
PsuI RGATCY 2 cut(s) 709, 1066
PsyI GACNNNGTC 1 cut(s) 128
PvuII CAGCTG 1 cut(s) 758
RsaI GTAC 3 cut(s) 257, 722, 941
RsaNI GTAC 3 cut(s) 256, 721, 940
RseI CAYNNNNRTG 1 cut(s) 522
SacI GAGCTC 1 cut(s) 8
SaqAI TTAA 6 cut(s) 509, 683, 698, 726, 993, 1172
SatI GCNGC 3 cut(s) 42, 88, 1101
Sau3AI GATC 7 cut(s) 157, 228, 280, 470, 709, 1066, 1267
SchI GAGTC 1 cut(s) 11
ScrFI CCNGG 1 cut(s) 278
SduI GDGCHC 2 cut(s) 8, 494
SfaNI GCATC 3 cut(s) 298, 1044, 1087
SfoI GGCGCC 2 cut(s) 262, 411
Sfr274I CTCGAG 1 cut(s) 149
SfuI TTCGAA 1 cut(s) 393
SgrAI CRCCGGYG 1 cut(s) 999
SlaI CTCGAG 1 cut(s) 149
SmiMI CAYNNNNRTG 1 cut(s) 522
SmlI CTYRAG 3 cut(s) 149, 725, 1238
SmoI CTYRAG 3 cut(s) 149, 725, 1238
SsiI CCGC 5 cut(s) 41, 88, 139, 193, 200
SspDI GGCGCC 2 cut(s) 260, 409
SspMI CTAG 2 cut(s) 302, 614
SstI GAGCTC 1 cut(s) 8
StyD4I CCNGG 1 cut(s) 276
StyI CCWWGG 2 cut(s) 771, 842
TaaI ACNGT 1 cut(s) 1047
TaiI ACGT 2 cut(s) 210, 237
TaqI TCGA 5 cut(s) 150, 231, 252, 393, 1059
TatI WGTACW 1 cut(s) 939
TauI GCSGC 2 cut(s) 44, 90
TfiI GAWTC 1 cut(s) 583
Tru1I TTAA 6 cut(s) 509, 683, 698, 726, 993, 1172
Tru9I TTAA 6 cut(s) 509, 683, 698, 726, 993, 1172
TscAI CASTG 2 cut(s) 874, 1280
TseFI GTSAC 2 cut(s) 658, 830
TseI GCWGC 1 cut(s) 1100
Tsp45I GTSAC 2 cut(s) 658, 830
TspDTI ATGAA 5 cut(s) 615, 704, 815, 1127, 1214
TspGWI ACGGA 1 cut(s) 704
TspRI CASTG 2 cut(s) 874, 1280
Tth111I GACNNNGTC 1 cut(s) 128
Vha464I CTTAAG 1 cut(s) 725
XapI RAATTY 4 cut(s) 607, 925, 1106, 1224
XcmI CCANNNNNNNNNTGG 1 cut(s) 884
XhoI CTCGAG 1 cut(s) 149
XmnI GAANNNNTTC 1 cut(s) 1257
XspI CTAG 2 cut(s) 302, 614
ZraI GACGTC 1 cut(s) 235
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.