Rroxscaffold_2G00117130

Uncharacterized protein family UPF0054

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0012694
Physical Location & Seq
Reverse (-)
44629387 .. 44636897
7511 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00117130.1

Sequence Viewer

Length: 450 bp
ATGAGCCAAAGGGGAGATCATGCCTTCGCTGAGCATCTCTTGGCTGCCGGTGACATACGTAAAGTAATCTTCATGGACACCGCCGAAGGAGTCGCAACTACTTTGAGGCCATACCGGTCGGAGCAATTAGAGATCGTGCAGCAGCTTGTTCAAGCTCAGCCGGACATGCTTGAAATTGTCCCTCCGGGGACCTCAAAAGGGAGTGGAGTAAAATTGCTGCTTGATCATTTGGGCATCACTCTAAAGGAGGTGATCAATGGATTGACTTGGCGAGATGATGGTGAATCGTTGATTGGGCTGTTAAGAAGACGTAGCGGGATTAGAGCTTTTGAGGTGAAACAAAGCCCAGACCAGTTCGCCGCTTCCCCACGAACAGCTCGGTGCCCTCCTTTCCTCCCTCACGGCGGCTACGCCGCGTTAAGGAGCAACACTTCCCTTGGTCTTGAGTAG

Protein Analysis

149

Amino Acids

16.29

Weight (kDa)

6.91

Isoelectric Point (pI)

50.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Hydrolase_3 PF08282 17 - 85 5.7e-07 haloacid dehalogenase-like hydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 381
AccII CGCG 1 cut(s) 416
AciI CCGC 5 cut(s) 81, 315, 360, 405, 414
AfiI CCNNNNNNNGG 3 cut(s) 198, 404, 420
AgeI ACCGGT 1 cut(s) 114
AgsI TTSAA 2 cut(s) 152, 173
AjuI GAANNNNNNNTTGG 2 cut(s) 276, 308
AluBI AGCT 4 cut(s) 145, 155, 326, 377
AluI AGCT 4 cut(s) 145, 155, 326, 377
AoxI GGCC 1 cut(s) 107
ApeKI GCWGC 4 cut(s) 44, 139, 142, 217
AsiGI ACCGGT 1 cut(s) 114
AspS9I GGNCC 1 cut(s) 189
AsuC2I CCSGG 1 cut(s) 186
AsuHPI GGTGA 4 cut(s) 62, 262, 293, 346
AvaII GGWCC 1 cut(s) 189
BaeGI GKGCMC 1 cut(s) 386
BanI GGYRCC 1 cut(s) 381
BbsI GAAGAC 1 cut(s) 313
BbvI GCAGC 4 cut(s) 31, 151, 154, 204
BccI CCATC 1 cut(s) 272
BceAI ACGGC 1 cut(s) 418
BclI TGATCA 2 cut(s) 223, 252
BcnI CCSGG 1 cut(s) 186
BisI GCNGC 7 cut(s) 45, 140, 143, 218, 360, 406, 414
BlpI GCTNAGC 2 cut(s) 30, 156
BlsI GCNGC 7 cut(s) 46, 141, 144, 219, 361, 407, 415
Bme1390I CCNGG 1 cut(s) 186
Bme18I GGWCC 1 cut(s) 189
BmgT120I GGNCC 1 cut(s) 189
BmiI GGNNCC 2 cut(s) 190, 383
BmrFI CCNGG 1 cut(s) 186
BmsI GCATC 2 cut(s) 43, 243
BpiI GAAGAC 1 cut(s) 313
Bpu1102I GCTNAGC 2 cut(s) 30, 156
BpuMI CCSGG 1 cut(s) 186
BsaAI YACGTR 1 cut(s) 59
BsaJI CCNNGG 2 cut(s) 185, 436
BsaWI WCCGGW 1 cut(s) 114
Bsc4I CCNNNNNNNGG 3 cut(s) 198, 404, 420
Bse118I RCCGGY 2 cut(s) 47, 114
Bse1I ACTGG 1 cut(s) 352
BseDI CCNNGG 2 cut(s) 185, 436
BseLI CCNNNNNNNGG 3 cut(s) 198, 404, 420
BseMII CTCAG 2 cut(s) 21, 170
BseNI ACTGG 1 cut(s) 352
BseSI GKGCMC 1 cut(s) 386
BseXI GCAGC 4 cut(s) 31, 151, 154, 204
BsgI GTGCAG 1 cut(s) 158
Bsh1236I CGCG 1 cut(s) 416
Bsh1285I CGRYCG 1 cut(s) 119
BshFI GGCC 1 cut(s) 109
BshNI GGYRCC 1 cut(s) 381
BshTI ACCGGT 1 cut(s) 114
BsiEI CGRYCG 1 cut(s) 119
BsiSI CCGG 4 cut(s) 48, 115, 161, 185
BslFI GGGAC 2 cut(s) 164, 202
BslI CCNNNNNNNGG 3 cut(s) 198, 404, 420
BsmFI GGGAC 2 cut(s) 164, 202
BsnI GGCC 1 cut(s) 109
Bsp1286I GDGCHC 1 cut(s) 386
Bsp143I GATC 4 cut(s) 16, 132, 223, 252
Bsp1720I GCTNAGC 2 cut(s) 30, 156
BspACI CCGC 5 cut(s) 81, 315, 360, 405, 414
BspANI GGCC 1 cut(s) 109
BspCNI CTCAG 2 cut(s) 22, 169
BspFNI CGCG 1 cut(s) 416
BspLI GGNNCC 2 cut(s) 190, 383
BspT107I GGYRCC 1 cut(s) 381
BsrFI RCCGGY 2 cut(s) 47, 114
BsrI ACTGG 1 cut(s) 352
BssAI RCCGGY 2 cut(s) 47, 114
BssECI CCNNGG 2 cut(s) 185, 436
BssMI GATC 4 cut(s) 16, 132, 223, 252
BssT1I CCWWGG 1 cut(s) 436
BstBAI YACGTR 1 cut(s) 59
BstDEI CTNAG 2 cut(s) 30, 156
BstFNI CGCG 1 cut(s) 416
BstKTI GATC 4 cut(s) 19, 135, 226, 255
BstMBI GATC 4 cut(s) 16, 132, 223, 252
BstMCI CGRYCG 1 cut(s) 119
BstMWI GCNNNNNNNGC 1 cut(s) 166
BstNSI RCATGY 1 cut(s) 169
BstSCI CCNGG 1 cut(s) 184
BstSLI GKGCMC 1 cut(s) 386
BstSNI TACGTA 1 cut(s) 59
BstUI CGCG 1 cut(s) 416
BstV1I GCAGC 4 cut(s) 31, 151, 154, 204
BstV2I GAAGAC 1 cut(s) 313
BsuRI GGCC 1 cut(s) 109
Cfr10I RCCGGY 2 cut(s) 47, 114
Cfr13I GGNCC 1 cut(s) 189
CspAI ACCGGT 1 cut(s) 114
CviAII CATG 3 cut(s) 20, 73, 166
DdeI CTNAG 2 cut(s) 30, 156
DpnI GATC 4 cut(s) 18, 134, 225, 254
DpnII GATC 4 cut(s) 16, 132, 223, 252
Eco105I TACGTA 1 cut(s) 59
Eco130I CCWWGG 1 cut(s) 436
Eco47I GGWCC 1 cut(s) 189
EcoO109I RGGNCCY 1 cut(s) 189
EcoT14I CCWWGG 1 cut(s) 436
ErhI CCWWGG 1 cut(s) 436
FaeI CATG 3 cut(s) 23, 76, 169
FaiI YATR 5 cut(s) 21, 56, 74, 112, 167
FaqI GGGAC 2 cut(s) 164, 202
FatI CATG 3 cut(s) 19, 72, 165
FauI CCCGC 1 cut(s) 308
FbaI TGATCA 2 cut(s) 223, 252
Fnu4HI GCNGC 7 cut(s) 45, 140, 143, 218, 360, 406, 414
Fsp4HI GCNGC 7 cut(s) 45, 140, 143, 218, 360, 406, 414
GluI GCNGC 7 cut(s) 45, 140, 143, 218, 360, 406, 414
HaeIII GGCC 1 cut(s) 109
HapII CCGG 4 cut(s) 48, 115, 161, 185
Hin1II CATG 3 cut(s) 23, 76, 169
HinfI GANTC 2 cut(s) 90, 284
HpaII CCGG 4 cut(s) 48, 115, 161, 185
HphI GGTGA 4 cut(s) 62, 262, 293, 346
Hpy188I TCNGA 1 cut(s) 121
Hpy188III TCNNGA 1 cut(s) 443
HpyAV CCTTC 2 cut(s) 34, 80
HpyCH4IV ACGT 2 cut(s) 58, 310
HpyCH4V TGCA 1 cut(s) 139
HpyF10VI GCNNNNNNNGC 1 cut(s) 166
HpyF3I CTNAG 2 cut(s) 30, 156
HpySE526I ACGT 2 cut(s) 58, 310
Hsp92II CATG 3 cut(s) 23, 76, 169
Ksp22I TGATCA 2 cut(s) 223, 252
Kzo9I GATC 4 cut(s) 16, 132, 223, 252
LmnI GCTCC 2 cut(s) 121, 423
LpnPI CCDG 6 cut(s) 61, 128, 174, 198, 360, 365
Lsp1109I GCAGC 4 cut(s) 31, 151, 154, 204
LweI GCATC 2 cut(s) 43, 243
MaeII ACGT 2 cut(s) 58, 310
MaeIII GTNAC 1 cut(s) 50
MalI GATC 4 cut(s) 18, 134, 225, 254
MboI GATC 4 cut(s) 16, 132, 223, 252
MboII GAAGA 2 cut(s) 61, 318
MhlI GDGCHC 1 cut(s) 386
MluCI AATT 3 cut(s) 125, 174, 212
MlyI GAGTC 1 cut(s) 99
MmeI TCCRAC 1 cut(s) 99
MnlI CCTC 8 cut(s) 99, 192, 202, 241, 325, 396, 404, 408
MseI TTAA 2 cut(s) 302, 419
MspI CCGG 4 cut(s) 48, 115, 161, 185
MspR9I CCNGG 1 cut(s) 186
MvnI CGCG 1 cut(s) 416
MwoI GCNNNNNNNGC 1 cut(s) 166
NciI CCSGG 1 cut(s) 186
NdeII GATC 4 cut(s) 16, 132, 223, 252
NlaIII CATG 3 cut(s) 23, 76, 169
NlaIV GGNNCC 2 cut(s) 190, 383
NmuCI GTSAC 1 cut(s) 50
NspI RCATGY 1 cut(s) 169
PfeI GAWTC 1 cut(s) 284
PinAI ACCGGT 1 cut(s) 114
PkrI GCNGC 7 cut(s) 46, 141, 144, 219, 361, 407, 415
PleI GAGTC 1 cut(s) 98
PpsI GAGTC 1 cut(s) 98
Ppu21I YACGTR 1 cut(s) 59
PpuMI RGGWCCY 1 cut(s) 189
Psp5II RGGWCCY 1 cut(s) 189
PspN4I GGNNCC 2 cut(s) 190, 383
PspPI GGNCC 1 cut(s) 189
PspPPI RGGWCCY 1 cut(s) 189
SaqAI TTAA 2 cut(s) 302, 419
SatI GCNGC 7 cut(s) 45, 140, 143, 218, 360, 406, 414
Sau3AI GATC 4 cut(s) 16, 132, 223, 252
Sau96I GGNCC 1 cut(s) 189
SchI GAGTC 1 cut(s) 99
ScrFI CCNGG 1 cut(s) 186
SduI GDGCHC 1 cut(s) 386
SetI ASST 9 cut(s) 61, 147, 157, 194, 252, 313, 328, 336, 379
SfaNI GCATC 2 cut(s) 43, 243
SinI GGWCC 1 cut(s) 189
SmlI CTYRAG 1 cut(s) 443
SmoI CTYRAG 1 cut(s) 443
SnaBI TACGTA 1 cut(s) 59
Sse9I AATT 3 cut(s) 125, 174, 212
SsiI CCGC 5 cut(s) 81, 315, 360, 405, 414
StyD4I CCNGG 1 cut(s) 184
StyI CCWWGG 1 cut(s) 436
TaiI ACGT 2 cut(s) 61, 313
TasI AATT 3 cut(s) 125, 174, 212
TauI GCSGC 3 cut(s) 362, 408, 416
TfiI GAWTC 1 cut(s) 284
Tru1I TTAA 2 cut(s) 302, 419
Tru9I TTAA 2 cut(s) 302, 419
TseFI GTSAC 1 cut(s) 50
TseI GCWGC 4 cut(s) 44, 139, 142, 217
Tsp45I GTSAC 1 cut(s) 50
TspDTI ATGAA 1 cut(s) 61
VpaK11BI GGWCC 1 cut(s) 189
XceI RCATGY 1 cut(s) 169
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.