Rroxscaffold_2G00136360

Acyl CoA binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0003519
Physical Location & Seq
Forward (+)
74079446 .. 74083268
3823 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00136360.1

Sequence Viewer

Length: 1608 bp
ATGGACTTTATTTCAGATTTTGTATTGACGATTACTCTGTGTTTCCTGGTACCTTTCTTGCTTTGCTTCCTTTTGCCTGTGATTGTTGCTTTGTGTCCGAGCAGGGATGACCATGTGGGAAAAGGACGGGTTCTTTATGACTGCAAGCCCAAAACTCATGAGTGGGAGAGTGAGGTAGGTAGTGGGTTTGTTGAGAAAGCTGTGGAAGGTGATGAGTTTTTGGGTGAGCCCAAAAAGGAAAGTTTGGTTCTTGAGGAGTTTGTGGATGCGGGTTTGGATGAAAATGAAGTTTTGAGAGGTGATGGAGTTGGTTGTTGGAAGGAGAAGAGTGTTGAGGAAGAAAGGTTTGATGGTTTGTGTGAGTGTGAATGTGTAGAGAACGTTTTTGATGAAAGTTCAGAGAGGTGTGAATTTGGGGGAGTTGAGTTTGATTTGGGGAAAAATGAAGTGGGGGTTGTTTGTTTTGGGGAGAAGAATGTTGAAGAAGAAGGCTTTGATGGGCATGGCGGTGTAGAGAACTTGTTTGATGAAAGTTGTGAGAGGCGTGAGTTTGGTTTGGGGAGAAGTGAAGTGGGGGTTGTTTGTTTGCCGGAGAAGAATGTTGAAGAAGAAAGCTTTGATGGGCGTGTTGGGGTATGTGGGTATGTAGATTACTTGGTTGATGAAAGTTCTGAGAGGCATGAAAATGAAAATGGCGGGCTTGAGTTTGATTTGGACCAAGCAGGAGTTGTTACTTTGCCTGAGAAGAATGTTGAACAAGAAAGCTGTGATGGGGATAGTGGTGTGTGTGGGTGTGGAGAGAACTTGGTTGATGAAAGTTCCGAGAGGCATGAATTTGGAGATATTGAGTCTGATTTAGCAAGAAATGGAGTGGGGGTTGTTTGTTTGCCGGAGAAGTATGTTGAGGAAGAATGCTCTGCTGGGGATGTTGGTATATGTGGCTGCGTGGAGAACTCGGTTGGTGCAAGTTCCGAGAAGCATGAATTTGGCAAAATTGAGTTTGGTTTGGCAAGAAACCAAGTGGGAATTCCGGAAAGTGAAGAGATCAATGTGTCAAAGTGTGATGGAAATGATGGAACTGATGAAGCCAAGAACAATGTAGTTGATGGTGAAGTTAATGAGGGGTTCTTCAGTGAGGATAATGATTGGGAGGATATAGAAAGAACTGAATTGGAGAGGCTCTTTGGTGCTGCAGTGGTGTTTGTCGGGTCTAAGAGTAATGCTGATAGAGTTTCGAGTCTTAGGAGTGATGTGAAGATGAGGTTGGATGGGCTTCACAAGATTGCTACTCGAGGGCCTTGCTTGGAACGCCAGCCTATGGCATTCAAGGTCTCGGCAAGAGCAAAATGGAATGCTTGGCAGCAGCTTGGCAACATGAGTGCAGAGGTAGCAATGGAGCAGTACATCACCCTTCTTTCAGAAAGTGTTCCTGGGTGGGTGCAAGATGTCCATGGTGACTTTACAGATCCCCAAGTATATGAGACGGTAACTGCTGATCTGAACACGTTGATTCAAAACCAATCAGAAGCTGCAGGTGTAAGAAAAGAAGAACTAAAGCCTGATGCTAAAGGTTTAGATGAGATTGGGGTGGGGGTCGTAACTTGTTGA

Protein Analysis

535

Amino Acids

58.7

Weight (kDa)

4.25

Isoelectric Point (pI)

43.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ACBP PF00887 393 - 472 2.2e-20 Acyl CoA binding protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1523
Acc36I ACCTGC 1 cut(s) 1523
Acc65I GGTACC 1 cut(s) 49
AccB1I GGYRCC 1 cut(s) 49
AccB7I CCANNNNNTGG 1 cut(s) 1318
AccIII TCCGGA 1 cut(s) 1030
AciI CCGC 3 cut(s) 269, 507, 696
AclI AACGTT 1 cut(s) 381
AclWI GGATC 1 cut(s) 1460
AcsI RAATTY 4 cut(s) 410, 833, 983, 1026
AcuI CTGAAG 1 cut(s) 1114
AfaI GTAC 2 cut(s) 51, 1403
AfiI CCNNNNNNNGG 1 cut(s) 1318
AflIII ACRYGT 1 cut(s) 1503
AgsI TTSAA 5 cut(s) 482, 605, 755, 1327, 1514
AjnI CCWGG 2 cut(s) 45, 1429
AjuI GAANNNNNNNTTGG 4 cut(s) 1011, 1043, 1247, 1279
AluBI AGCT 5 cut(s) 200, 615, 765, 1366, 1529
AluI AGCT 5 cut(s) 200, 615, 765, 1366, 1529
Alw26I GTCTC 2 cut(s) 1336, 1475
AlwI GGATC 1 cut(s) 1460
AlwNI CAGNNNCTG 1 cut(s) 1529
Ama87I CYCGRG 1 cut(s) 1290
Aor13HI TCCGGA 1 cut(s) 1030
AoxI GGCC 1 cut(s) 1295
ApeKI GCWGC 5 cut(s) 942, 1190, 1360, 1363, 1529
ApoI RAATTY 4 cut(s) 410, 833, 983, 1026
Asp700I GAANNNNTTC 1 cut(s) 1425
Asp718I GGTACC 1 cut(s) 49
AspS9I GGNCC 2 cut(s) 715, 1295
AsuHPI GGTGA 6 cut(s) 221, 236, 311, 1121, 1399, 1466
AvaI CYCGRG 1 cut(s) 1290
AvaII GGWCC 1 cut(s) 715
BanI GGYRCC 1 cut(s) 49
BanII GRGCYC 1 cut(s) 231
BbvI GCAGC 5 cut(s) 929, 1177, 1372, 1375, 1516
BccI CCATC 9 cut(s) 296, 344, 491, 614, 764, 1058, 1067, 1100, 1262
BcgI CGANNNNNNTGC 2 cut(s) 1281, 1315
BciT130I CCWGG 2 cut(s) 47, 1431
BcoDI GTCTC 2 cut(s) 1336, 1475
BfmI CTRYAG 2 cut(s) 1191, 1530
BfuAI ACCTGC 1 cut(s) 1523
BisI GCNGC 5 cut(s) 943, 1191, 1361, 1364, 1530
BlsI GCNGC 5 cut(s) 944, 1192, 1362, 1365, 1531
Bme1390I CCNGG 2 cut(s) 47, 1431
Bme18I GGWCC 1 cut(s) 715
BmeT110I CYCGRG 1 cut(s) 1290
BmgT120I GGNCC 2 cut(s) 715, 1295
BmiI GGNNCC 1 cut(s) 51
BmrFI CCNGG 2 cut(s) 47, 1431
BmsI GCATC 2 cut(s) 256, 1552
BpuEI CTTGAG 2 cut(s) 272, 722
BsaI GGTCTC 1 cut(s) 1336
BsaJI CCNNGG 2 cut(s) 1430, 1450
BsaWI WCCGGW 1 cut(s) 1030
BsaXI ACNNNNNCTCC 2 cut(s) 861, 891
Bsc4I CCNNNNNNNGG 1 cut(s) 1318
Bse3DI GCAATG 1 cut(s) 1398
BseAI TCCGGA 1 cut(s) 1030
BseBI CCWGG 2 cut(s) 47, 1431
BseDI CCNNGG 2 cut(s) 1430, 1450
BseGI GGATG 5 cut(s) 112, 271, 283, 931, 1273
BseLI CCNNNNNNNGG 1 cut(s) 1318
BseMI GCAATG 1 cut(s) 1398
BseMII CTCAG 2 cut(s) 663, 732
BseRI GAGGAG 1 cut(s) 269
BseXI GCAGC 5 cut(s) 929, 1177, 1372, 1375, 1516
BseYI CCCAGC 1 cut(s) 920
BsgI GTGCAG 1 cut(s) 1401
BshFI GGCC 1 cut(s) 1297
BshNI GGYRCC 1 cut(s) 49
BsiHKCI CYCGRG 1 cut(s) 1290
BsiSI CCGG 3 cut(s) 590, 890, 1031
BslI CCNNNNNNNGG 1 cut(s) 1318
BsmAI GTCTC 2 cut(s) 1336, 1475
BsmBI CGTCTC 1 cut(s) 1475
BsmI GAATGC 3 cut(s) 917, 1322, 1357
BsnI GGCC 1 cut(s) 1297
Bso31I GGTCTC 1 cut(s) 1336
BsoBI CYCGRG 1 cut(s) 1290
Bsp1286I GDGCHC 1 cut(s) 231
Bsp13I TCCGGA 1 cut(s) 1030
Bsp143I GATC 3 cut(s) 1044, 1465, 1495
Bsp19I CCATGG 1 cut(s) 1450
BspACI CCGC 3 cut(s) 269, 507, 696
BspANI GGCC 1 cut(s) 1297
BspCNI CTCAG 2 cut(s) 664, 733
BspEI TCCGGA 1 cut(s) 1030
BspHI TCATGA 1 cut(s) 157
BspLI GGNNCC 1 cut(s) 51
BspMAI CTGCAG 2 cut(s) 1195, 1534
BspMI ACCTGC 1 cut(s) 1523
BspPI GGATC 1 cut(s) 1460
BspT107I GGYRCC 1 cut(s) 49
BspTNI GGTCTC 1 cut(s) 1336
BsrDI GCAATG 1 cut(s) 1398
BssECI CCNNGG 2 cut(s) 1430, 1450
BssMI GATC 3 cut(s) 1044, 1465, 1495
BssT1I CCWWGG 1 cut(s) 1450
Bst2UI CCWGG 2 cut(s) 47, 1431
Bst4CI ACNGT 1 cut(s) 1486
Bst6I CTCTTC 2 cut(s) 320, 1035
BstC8I GCNNGC 3 cut(s) 146, 698, 1313
BstDEI CTNAG 4 cut(s) 672, 741, 1212, 1241
BstDSI CCRYGG 1 cut(s) 1450
BstF5I GGATG 5 cut(s) 112, 271, 283, 931, 1273
BstKTI GATC 3 cut(s) 1047, 1468, 1498
BstMAI GTCTC 2 cut(s) 1336, 1475
BstMBI GATC 3 cut(s) 1044, 1465, 1495
BstMWI GCNNNNNNNGC 2 cut(s) 1308, 1388
BstNI CCWGG 2 cut(s) 47, 1431
BstSCI CCNGG 2 cut(s) 45, 1429
BstSFI CTRYAG 2 cut(s) 1191, 1530
BstV1I GCAGC 5 cut(s) 929, 1177, 1372, 1375, 1516
BstX2I RGATCY 1 cut(s) 1465
BstYI RGATCY 1 cut(s) 1465
BsuRI GGCC 1 cut(s) 1297
BtgI CCRYGG 1 cut(s) 1450
BtsCI GGATG 5 cut(s) 112, 271, 283, 931, 1273
BtsI GCAGTG 1 cut(s) 1200
BtsIMutI CAGTG 2 cut(s) 1138, 1200
BveI ACCTGC 1 cut(s) 1523
Cac8I GCNNGC 3 cut(s) 146, 698, 1313
CaiI CAGNNNCTG 1 cut(s) 1529
CciI TCATGA 1 cut(s) 157
Cfr13I GGNCC 2 cut(s) 715, 1295
Csp6I GTAC 2 cut(s) 50, 1402
CviAII CATG 8 cut(s) 113, 158, 503, 680, 830, 980, 1375, 1451
CviQI GTAC 2 cut(s) 50, 1402
DdeI CTNAG 4 cut(s) 672, 741, 1212, 1241
DpnI GATC 3 cut(s) 1046, 1467, 1497
DpnII GATC 3 cut(s) 1044, 1465, 1495
Eam1104I CTCTTC 2 cut(s) 320, 1035
EarI CTCTTC 2 cut(s) 320, 1035
Eco130I CCWWGG 1 cut(s) 1450
Eco24I GRGCYC 1 cut(s) 231
Eco31I GGTCTC 1 cut(s) 1336
Eco47I GGWCC 1 cut(s) 715
Eco57I CTGAAG 1 cut(s) 1114
Eco88I CYCGRG 1 cut(s) 1290
EcoO109I RGGNCCY 1 cut(s) 1295
EcoRI GAATTC 1 cut(s) 1026
EcoRII CCWGG 2 cut(s) 45, 1429
EcoT14I CCWWGG 1 cut(s) 1450
EcoT38I GRGCYC 1 cut(s) 231
ErhI CCWWGG 1 cut(s) 1450
Esp3I CGTCTC 1 cut(s) 1475
FaeI CATG 8 cut(s) 116, 161, 506, 683, 833, 983, 1378, 1454
FatI CATG 8 cut(s) 112, 157, 502, 679, 829, 979, 1374, 1450
FauI CCCGC 2 cut(s) 262, 689
Fnu4HI GCNGC 5 cut(s) 943, 1191, 1361, 1364, 1530
FokI GGATG 5 cut(s) 119, 278, 290, 938, 1280
FriOI GRGCYC 1 cut(s) 231
Fsp4HI GCNGC 5 cut(s) 943, 1191, 1361, 1364, 1530
GluI GCNGC 5 cut(s) 943, 1191, 1361, 1364, 1530
GsaI CCCAGC 1 cut(s) 924
HaeIII GGCC 1 cut(s) 1297
HapII CCGG 3 cut(s) 590, 890, 1031
Hin1II CATG 8 cut(s) 116, 161, 506, 683, 833, 983, 1378, 1454
HindIII AAGCTT 1 cut(s) 613
HinfI GANTC 3 cut(s) 848, 1237, 1510
HpaII CCGG 3 cut(s) 590, 890, 1031
HphI GGTGA 6 cut(s) 221, 236, 311, 1121, 1399, 1466
Hpy188III TCNNGA 3 cut(s) 158, 251, 1031
HpyAV CCTTC 4 cut(s) 200, 313, 482, 1421
HpyCH4III ACNGT 1 cut(s) 1486
HpyCH4IV ACGT 2 cut(s) 381, 1505
HpyCH4V TGCA 6 cut(s) 144, 965, 1193, 1382, 1441, 1532
HpyF10VI GCNNNNNNNGC 2 cut(s) 1308, 1388
HpyF3I CTNAG 4 cut(s) 672, 741, 1212, 1241
HpySE526I ACGT 2 cut(s) 381, 1505
Hsp92II CATG 8 cut(s) 116, 161, 506, 683, 833, 983, 1378, 1454
Kpn2I TCCGGA 1 cut(s) 1030
KpnI GGTACC 1 cut(s) 53
Kzo9I GATC 3 cut(s) 1044, 1465, 1495
LmnI GCTCC 1 cut(s) 1396
Lsp1109I GCAGC 5 cut(s) 929, 1177, 1372, 1375, 1516
LweI GCATC 2 cut(s) 256, 1552
MaeII ACGT 2 cut(s) 381, 1505
MaeIII GTNAC 4 cut(s) 730, 1454, 1486, 1597
MalI GATC 3 cut(s) 1046, 1467, 1497
MboI GATC 3 cut(s) 1044, 1465, 1495
MflI RGATCY 1 cut(s) 1465
MhlI GDGCHC 1 cut(s) 231
MluCI AATT 6 cut(s) 410, 833, 983, 993, 1026, 1169
MlyI GAGTC 2 cut(s) 857, 1246
MmeI TCCRAC 2 cut(s) 296, 1245
MroI TCCGGA 1 cut(s) 1030
MroXI GAANNNNTTC 1 cut(s) 1425
MseI TTAA 1 cut(s) 1116
MslI CAYNNNNRTG 2 cut(s) 507, 684
MspI CCGG 3 cut(s) 590, 890, 1031
MspR9I CCNGG 2 cut(s) 47, 1431
Mva1269I GAATGC 3 cut(s) 917, 1322, 1357
MvaI CCWGG 2 cut(s) 47, 1431
MwoI GCNNNNNNNGC 2 cut(s) 1308, 1388
NcoI CCATGG 1 cut(s) 1450
NdeII GATC 3 cut(s) 1044, 1465, 1495
NlaIII CATG 8 cut(s) 116, 161, 506, 683, 833, 983, 1378, 1454
NlaIV GGNNCC 1 cut(s) 51
NmeAIII GCCGAG 1 cut(s) 1313
NmuCI GTSAC 1 cut(s) 1454
PaeR7I CTCGAG 1 cut(s) 1290
PagI TCATGA 1 cut(s) 157
PaqCI CACCTGC 1 cut(s) 1523
PctI GAATGC 3 cut(s) 917, 1322, 1357
PdmI GAANNNNTTC 1 cut(s) 1425
PfeI GAWTC 1 cut(s) 1510
PflMI CCANNNNNTGG 1 cut(s) 1318
PkrI GCNGC 5 cut(s) 944, 1192, 1362, 1365, 1531
PleI GAGTC 2 cut(s) 856, 1245
PpsI GAGTC 2 cut(s) 856, 1245
Psp1406I AACGTT 1 cut(s) 381
Psp6I CCWGG 2 cut(s) 45, 1429
PspFI CCCAGC 1 cut(s) 920
PspGI CCWGG 2 cut(s) 45, 1429
PspN4I GGNNCC 1 cut(s) 51
PspPI GGNCC 2 cut(s) 715, 1295
PspXI VCTCGAGB 1 cut(s) 1290
PstI CTGCAG 2 cut(s) 1195, 1534
PstNI CAGNNNCTG 1 cut(s) 1529
PsuI RGATCY 1 cut(s) 1465
RsaI GTAC 2 cut(s) 51, 1403
RsaNI GTAC 2 cut(s) 50, 1402
RseI CAYNNNNRTG 2 cut(s) 507, 684
SaqAI TTAA 1 cut(s) 1116
SatI GCNGC 5 cut(s) 943, 1191, 1361, 1364, 1530
Sau3AI GATC 3 cut(s) 1044, 1465, 1495
Sau96I GGNCC 2 cut(s) 715, 1295
SchI GAGTC 2 cut(s) 857, 1246
ScrFI CCNGG 2 cut(s) 47, 1431
SduI GDGCHC 1 cut(s) 231
SfaNI GCATC 2 cut(s) 256, 1552
SfcI CTRYAG 2 cut(s) 1191, 1530
Sfr274I CTCGAG 1 cut(s) 1290
SinI GGWCC 1 cut(s) 715
SlaI CTCGAG 1 cut(s) 1290
SmiMI CAYNNNNRTG 2 cut(s) 507, 684
SmlI CTYRAG 3 cut(s) 251, 701, 1290
SmoI CTYRAG 3 cut(s) 251, 701, 1290
Sse9I AATT 6 cut(s) 410, 833, 983, 993, 1026, 1169
SsiI CCGC 3 cut(s) 269, 507, 696
StyD4I CCNGG 2 cut(s) 45, 1429
StyI CCWWGG 1 cut(s) 1450
TaaI ACNGT 1 cut(s) 1486
TaiI ACGT 2 cut(s) 384, 1508
TaqI TCGA 2 cut(s) 1235, 1291
TasI AATT 6 cut(s) 410, 833, 983, 993, 1026, 1169
TatI WGTACW 1 cut(s) 1401
TfiI GAWTC 1 cut(s) 1510
Tru1I TTAA 1 cut(s) 1116
Tru9I TTAA 1 cut(s) 1116
TscAI CASTG 2 cut(s) 1138, 1200
TseFI GTSAC 1 cut(s) 1454
TseI GCWGC 5 cut(s) 942, 1190, 1360, 1363, 1529
Tsp45I GTSAC 1 cut(s) 1454
TspRI CASTG 2 cut(s) 1138, 1200
Van91I CCANNNNNTGG 1 cut(s) 1318
VpaK11BI GGWCC 1 cut(s) 715
XapI RAATTY 4 cut(s) 410, 833, 983, 1026
XhoI CTCGAG 1 cut(s) 1290
XmnI GAANNNNTTC 1 cut(s) 1425
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.