Rroxscaffold_3G00222590

VQ motif

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0013152
Physical Location & Seq
Reverse (-)
5614725 .. 5615756
1032 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00222590.1

Sequence Viewer

Length: 1032 bp
ATGGATAAGACCTCATCTTCTTCTACTGAAAACTCCACGGGTATCACTAGGGATCCGTACCTGAAACACCTCAACAAGCTTTCCCACAAGATCTCCAAGCCCATAATCCGAAAACCCAATTTCGACCCGCCTCCTCCTCCTCCTCCGCCTCCCGCTCCGATTCCCAACCCCCAAAACCAAAACGCACCGCTTCAGCTCCAATCCTCTGCTTCGCAGCCTCAGCAACCCCCACAGCAGCCGCCGAACCAGCACCAGCCTCCGGTCTACAACATCAACAAGAACGATTTCAGGGATGTGGTTCAGAAGCTGACCGGTTCGCCGGCCCACACGCCGGACCGGTTCGCCACTCCTCCGCCGATTCACCCGCCCAAAACCCAGAGCTCGAGGCTCCAGCGCATCCGCCCTCCGCCGCTGGCCCAGCTCGCCGCGCGGCCGCCTTCTCTTCTCGACGGCGCCGTTTCGGCTCAGCCGAACAACACCACCACCAACAACAACAACAACCCTCTCATTCCGCCCATGGGAACCGCCGCGGCTCATTTCGGCGGCGTGATCGGACGGTCCACGGCGCCGCTGTCTCCTCTGCCTCCTTTTCCGAGCGTCCACGCGGCGGCGGAGTCGCCGGTCTCGGCCTACATGAGGTTCCTCCACAACTCGCTCTCCGCCGGCGATTCTAATCAGAACATGAACCAGAAGCAGCAGCATTTCTCTGGATTTTCTCCCTTGGCTCCACTCGTCTCGCCTCGCTGGACCAACCTGACGCCACCGCAACCGGAGCAGAGTCAGAATCAGAATCAGAGCCAGCAGCTTCCTCAACCGGAGCAGGCTATGGCTCCCTCGTCGGAGCCTCCACAACCTGCACAGGTTGCTTCTTCCCAGCCCCAATTCGCTATGCCACCGTCGCCGCTTCCATTTGGATGCTTGAATTCACCACGGTCGCAGTATAATCTGCTTTCGCCGAGTCAGTTAGGGTTTCCTCAACTCCCTCTGTCGCCGACTGTGCCGGTTCCAAGCCCTAGGTGGAGGGGCTTCTAA

Protein Analysis

343

Amino Acids

37.1

Weight (kDa)

10.23

Isoelectric Point (pI)

88.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
VQ PF05678 84 - 109 4.2e-10 VQ motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013152)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G78310
fragaria_vesca FvH4_5g36700
malus_domestica MD08G1187500.v1.1 MD15G1373500.v1.1
prunus_persica Prupe.1G522100_v2.0.a1
pyrus_communis pycom15g33460
rosa_chinensis RchiOBHm_Chr7g0242681
rosa_laevigata RLG00000000888
rosa_multiflora Rmu_co8298747.1_g000001 Rmu_sc0014793.1_g000001
rosa_roxburghii Rroxscaffold_3G00222590
rosa_rugosa Rorug07G0316200
rosa_samantha Rh7AG471200 Rh7BG441500 Rh7CG488200 Rh7DG456800
rosa_wichuraiana Rw0G005450 Rw7G039240

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 862
AccB1I GGYRCC 2 cut(s) 452, 565
AccBSI CCGCTC 1 cut(s) 155
AccI GTMKAC 1 cut(s) 264
AccII CGCG 4 cut(s) 428, 430, 530, 605
AclWI GGATC 2 cut(s) 47, 60
AcoI YGGCCR 1 cut(s) 431
AcsI RAATTY 1 cut(s) 922
AcuI CTGAAG 1 cut(s) 176
AcyI GRCGYC 3 cut(s) 453, 566, 758
AfaI GTAC 1 cut(s) 59
AfiI CCNNNNNNNGG 5 cut(s) 259, 331, 518, 607, 636
AgeI ACCGGT 2 cut(s) 311, 336
AgsI TTSAA 1 cut(s) 922
AluBI AGCT 6 cut(s) 79, 196, 307, 381, 421, 805
AluI AGCT 6 cut(s) 79, 196, 307, 381, 421, 805
Alw21I GWGCWC 1 cut(s) 383
Alw26I GTCTC 3 cut(s) 579, 628, 739
AlwI GGATC 2 cut(s) 47, 60
AlwNI CAGNNNCTG 1 cut(s) 307
Ama87I CYCGRG 1 cut(s) 382
AoxI GGCC 4 cut(s) 321, 414, 431, 627
ApeKI GCWGC 5 cut(s) 214, 235, 694, 697, 802
ApoI RAATTY 1 cut(s) 922
AsiGI ACCGGT 2 cut(s) 311, 336
Asp700I GAANNNNTTC 1 cut(s) 284
AspA2I CCTAGG 1 cut(s) 1013
AspLEI GCGC 4 cut(s) 396, 430, 455, 568
AspS9I GGNCC 5 cut(s) 322, 334, 415, 558, 747
AsuHPI GGTGA 2 cut(s) 353, 918
AvaI CYCGRG 1 cut(s) 382
AvaII GGWCC 3 cut(s) 334, 558, 747
AvrII CCTAGG 1 cut(s) 1013
BamHI GGATCC 1 cut(s) 52
BanI GGYRCC 2 cut(s) 452, 565
BanII GRGCYC 1 cut(s) 383
Bbv12I GWGCWC 1 cut(s) 383
BbvCI CCTCAGC 1 cut(s) 219
BbvI GCAGC 5 cut(s) 226, 247, 706, 709, 814
BceAI ACGGC 3 cut(s) 440, 466, 579
BcoDI GTCTC 3 cut(s) 579, 628, 739
BfaI CTAG 2 cut(s) 48, 1014
BfoI RGCGCY 2 cut(s) 456, 569
BfuAI ACCTGC 1 cut(s) 862
BglI GCCNNNNNGGC 1 cut(s) 461
BglII AGATCT 1 cut(s) 90
BlnI CCTAGG 1 cut(s) 1013
BlpI GCTNAGC 1 cut(s) 465
Bme18I GGWCC 3 cut(s) 334, 558, 747
BmeT110I CYCGRG 1 cut(s) 382
BmgT120I GGNCC 5 cut(s) 322, 334, 415, 558, 747
BmsI GCATC 2 cut(s) 405, 905
BpmI CTGGAG 1 cut(s) 374
Bpu10I CCTNAGC 1 cut(s) 219
Bpu1102I GCTNAGC 1 cut(s) 465
BsaBI GATNNNNATC 1 cut(s) 672
BsaHI GRCGYC 3 cut(s) 453, 566, 758
BsaI GGTCTC 1 cut(s) 628
BsaJI CCNNGG 7 cut(s) 36, 516, 528, 561, 720, 929, 1013
BsaWI WCCGGW 5 cut(s) 259, 311, 336, 769, 814
BsaXI ACNNNNNCTCC 4 cut(s) 77, 107, 641, 671
Bsc4I CCNNNNNNNGG 5 cut(s) 259, 331, 518, 607, 636
Bse118I RCCGGY 6 cut(s) 311, 319, 336, 619, 662, 1000
Bse8I GATNNNNATC 1 cut(s) 672
BseDI CCNNGG 7 cut(s) 36, 516, 528, 561, 720, 929, 1013
BseGI GGATG 3 cut(s) 298, 396, 920
BseJI GATNNNNATC 1 cut(s) 672
BseLI CCNNNNNNNGG 5 cut(s) 259, 331, 518, 607, 636
BseMII CTCAG 2 cut(s) 233, 479
BseRI GAGGAG 6 cut(s) 123, 126, 129, 132, 339, 567
BseX3I CGGCCG 1 cut(s) 431
BseXI GCAGC 5 cut(s) 226, 247, 706, 709, 814
BseYI CCCAGC 2 cut(s) 417, 873
BsgI GTGCAG 1 cut(s) 840
Bsh1236I CGCG 4 cut(s) 428, 430, 530, 605
Bsh1285I CGRYCG 2 cut(s) 434, 935
BshFI GGCC 4 cut(s) 323, 416, 433, 629
BshNI GGYRCC 2 cut(s) 452, 565
BshTI ACCGGT 2 cut(s) 311, 336
BsiEI CGRYCG 2 cut(s) 434, 935
BsiHKAI GWGCWC 1 cut(s) 383
BsiHKCI CYCGRG 1 cut(s) 382
BslI CCNNNNNNNGG 5 cut(s) 259, 331, 518, 607, 636
BsmAI GTCTC 3 cut(s) 579, 628, 739
BsmBI CGTCTC 1 cut(s) 739
BsnI GGCC 4 cut(s) 323, 416, 433, 629
Bso31I GGTCTC 1 cut(s) 628
BsoBI CYCGRG 1 cut(s) 382
Bsp1286I GDGCHC 1 cut(s) 383
Bsp143I GATC 3 cut(s) 52, 90, 549
Bsp1720I GCTNAGC 1 cut(s) 465
Bsp19I CCATGG 1 cut(s) 516
BspANI GGCC 4 cut(s) 323, 416, 433, 629
BspCNI CTCAG 2 cut(s) 232, 478
BspFNI CGCG 4 cut(s) 428, 430, 530, 605
BspMI ACCTGC 1 cut(s) 862
BspPI GGATC 2 cut(s) 47, 60
BspT107I GGYRCC 2 cut(s) 452, 565
BspTNI GGTCTC 1 cut(s) 628
BsrBI CCGCTC 1 cut(s) 155
BsrFI RCCGGY 6 cut(s) 311, 319, 336, 619, 662, 1000
BssAI RCCGGY 6 cut(s) 311, 319, 336, 619, 662, 1000
BssECI CCNNGG 7 cut(s) 36, 516, 528, 561, 720, 929, 1013
BssMI GATC 3 cut(s) 52, 90, 549
BssNI GRCGYC 3 cut(s) 453, 566, 758
BssT1I CCWWGG 3 cut(s) 516, 720, 1013
Bst4CI ACNGT 4 cut(s) 558, 897, 933, 997
Bst6I CTCTTC 1 cut(s) 447
BstACI GRCGYC 3 cut(s) 453, 566, 758
BstAPI GCANNNNNTGC 1 cut(s) 863
BstC8I GCNNGC 6 cut(s) 321, 414, 423, 664, 800, 822
BstDEI CTNAG 2 cut(s) 219, 465
BstDSI CCRYGG 5 cut(s) 36, 516, 528, 561, 929
BstENI CCTNNNNNAGG 1 cut(s) 634
BstF5I GGATG 3 cut(s) 298, 396, 920
BstFNI CGCG 4 cut(s) 428, 430, 530, 605
BstH2I RGCGCY 2 cut(s) 456, 569
BstHHI GCGC 4 cut(s) 396, 430, 455, 568
BstKTI GATC 3 cut(s) 55, 93, 552
BstMAI GTCTC 3 cut(s) 579, 628, 739
BstMBI GATC 3 cut(s) 52, 90, 549
BstMCI CGRYCG 2 cut(s) 434, 935
BstUI CGCG 4 cut(s) 428, 430, 530, 605
BstV1I GCAGC 5 cut(s) 226, 247, 706, 709, 814
BstX2I RGATCY 2 cut(s) 52, 90
BstYI RGATCY 2 cut(s) 52, 90
BstZI CGGCCG 1 cut(s) 431
BsuRI GGCC 4 cut(s) 323, 416, 433, 629
BtgI CCRYGG 5 cut(s) 36, 516, 528, 561, 929
BtsCI GGATG 3 cut(s) 298, 396, 920
BveI ACCTGC 1 cut(s) 862
Cac8I GCNNGC 6 cut(s) 321, 414, 423, 664, 800, 822
CaiI CAGNNNCTG 1 cut(s) 307
CciNI GCGGCCGC 1 cut(s) 431
CfoI GCGC 4 cut(s) 396, 430, 455, 568
Cfr10I RCCGGY 6 cut(s) 311, 319, 336, 619, 662, 1000
Cfr13I GGNCC 5 cut(s) 322, 334, 415, 558, 747
Cfr42I CCGCGG 1 cut(s) 531
CpoI CGGWCCG 1 cut(s) 334
CseI GACGC 2 cut(s) 586, 766
Csp6I GTAC 1 cut(s) 58
CspAI ACCGGT 2 cut(s) 311, 336
CspI CGGWCCG 1 cut(s) 334
CviAII CATG 3 cut(s) 517, 634, 682
CviQI GTAC 1 cut(s) 58
DdeI CTNAG 2 cut(s) 219, 465
DinI GGCGCC 2 cut(s) 454, 567
DpnI GATC 3 cut(s) 54, 92, 551
DpnII GATC 3 cut(s) 52, 90, 549
EaeI YGGCCR 1 cut(s) 431
EagI CGGCCG 1 cut(s) 431
Eam1104I CTCTTC 1 cut(s) 447
EarI CTCTTC 1 cut(s) 447
EciI GGCGGA 7 cut(s) 135, 342, 389, 396, 501, 626, 649
Ecl136II GAGCTC 1 cut(s) 381
EclXI CGGCCG 1 cut(s) 431
Eco130I CCWWGG 3 cut(s) 516, 720, 1013
Eco24I GRGCYC 1 cut(s) 383
Eco31I GGTCTC 1 cut(s) 628
Eco47I GGWCC 3 cut(s) 334, 558, 747
Eco52I CGGCCG 1 cut(s) 431
Eco53kI GAGCTC 1 cut(s) 381
Eco57I CTGAAG 1 cut(s) 176
Eco88I CYCGRG 1 cut(s) 382
EcoICRI GAGCTC 1 cut(s) 381
EcoNI CCTNNNNNAGG 1 cut(s) 634
EcoRI GAATTC 1 cut(s) 922
EcoT14I CCWWGG 3 cut(s) 516, 720, 1013
EcoT38I GRGCYC 1 cut(s) 383
EgeI GGCGCC 2 cut(s) 454, 567
EheI GGCGCC 2 cut(s) 454, 567
ErhI CCWWGG 3 cut(s) 516, 720, 1013
Esp3I CGTCTC 1 cut(s) 739
FaeI CATG 3 cut(s) 520, 637, 685
FaiI YATR 7 cut(s) 104, 518, 635, 683, 827, 890, 942
FatI CATG 3 cut(s) 516, 633, 681
FauI CCCGC 3 cut(s) 135, 160, 372
FblI GTMKAC 1 cut(s) 264
FokI GGATG 3 cut(s) 305, 383, 927
FriOI GRGCYC 1 cut(s) 383
FspBI CTAG 2 cut(s) 48, 1014
GlaI GCGC 4 cut(s) 395, 429, 454, 567
GsaI CCCAGC 2 cut(s) 421, 877
GsuI CTGGAG 1 cut(s) 374
HaeII RGCGCY 2 cut(s) 456, 569
HaeIII GGCC 4 cut(s) 323, 416, 433, 629
HgaI GACGC 2 cut(s) 586, 766
HhaI GCGC 4 cut(s) 396, 430, 455, 568
Hin1I GRCGYC 3 cut(s) 453, 566, 758
Hin1II CATG 3 cut(s) 520, 637, 685
Hin6I GCGC 4 cut(s) 394, 428, 453, 566
HinP1I GCGC 4 cut(s) 394, 428, 453, 566
HindIII AAGCTT 1 cut(s) 77
HinfI GANTC 8 cut(s) 160, 358, 614, 668, 778, 784, 790, 958
HphI GGTGA 2 cut(s) 353, 918
Hpy166II GTNNAC 3 cut(s) 265, 561, 601
Hpy188III TCNNGA 2 cut(s) 446, 708
Hpy8I GTNNAC 3 cut(s) 265, 561, 601
Hpy99I CGWCG 3 cut(s) 452, 841, 901
HpyAV CCTTC 1 cut(s) 447
HpyCH4III ACNGT 4 cut(s) 558, 897, 933, 997
HpyCH4V TGCA 1 cut(s) 857
HpyF3I CTNAG 2 cut(s) 219, 465
Hsp92I GRCGYC 3 cut(s) 453, 566, 758
Hsp92II CATG 3 cut(s) 520, 637, 685
HspAI GCGC 4 cut(s) 394, 428, 453, 566
KasI GGCGCC 2 cut(s) 452, 565
KroI GCCGGC 2 cut(s) 319, 662
KroNI GCCGGC 2 cut(s) 321, 664
KspI CCGCGG 1 cut(s) 531
Kzo9I GATC 3 cut(s) 52, 90, 549
LmnI GCTCC 8 cut(s) 160, 201, 393, 730, 772, 817, 835, 841
Lsp1109I GCAGC 5 cut(s) 226, 247, 706, 709, 814
LweI GCATC 2 cut(s) 405, 905
MaeI CTAG 2 cut(s) 48, 1014
MalI GATC 3 cut(s) 54, 92, 551
MbiI CCGCTC 1 cut(s) 155
MboI GATC 3 cut(s) 52, 90, 549
MboII GAAGA 4 cut(s) 9, 12, 434, 861
MflI RGATCY 2 cut(s) 52, 90
MhlI GDGCHC 1 cut(s) 383
MluCI AATT 3 cut(s) 118, 881, 922
Mly113I GGCGCC 2 cut(s) 453, 566
MlyI GAGTC 3 cut(s) 623, 787, 967
MmeI TCCRAC 1 cut(s) 819
MreI CGCCGGCG 1 cut(s) 662
MroNI GCCGGC 2 cut(s) 319, 662
MroXI GAANNNNTTC 1 cut(s) 284
MslI CAYNNNNRTG 1 cut(s) 913
MspA1I CMGCKG 3 cut(s) 412, 530, 571
MvnI CGCG 4 cut(s) 428, 430, 530, 605
NaeI GCCGGC 2 cut(s) 321, 664
NarI GGCGCC 2 cut(s) 453, 566
NcoI CCATGG 1 cut(s) 516
NdeII GATC 3 cut(s) 52, 90, 549
NgoMIV GCCGGC 2 cut(s) 319, 662
NlaIII CATG 3 cut(s) 520, 637, 685
NmeAIII GCCGAG 2 cut(s) 605, 981
NotI GCGGCCGC 1 cut(s) 431
PaeR7I CTCGAG 1 cut(s) 382
PcsI WCGNNNNNNNCGW 2 cut(s) 453, 467
PdiI GCCGGC 2 cut(s) 321, 664
PdmI GAANNNNTTC 1 cut(s) 284
PfeI GAWTC 5 cut(s) 160, 358, 668, 784, 790
PinAI ACCGGT 2 cut(s) 311, 336
PleI GAGTC 3 cut(s) 622, 786, 966
PluTI GGCGCC 2 cut(s) 456, 569
PpsI GAGTC 3 cut(s) 622, 786, 966
Psp124BI GAGCTC 1 cut(s) 383
PspFI CCCAGC 2 cut(s) 417, 873
PspPI GGNCC 5 cut(s) 322, 334, 415, 558, 747
PspXI VCTCGAGB 1 cut(s) 382
PstNI CAGNNNCTG 1 cut(s) 307
PsuI RGATCY 2 cut(s) 52, 90
RsaI GTAC 1 cut(s) 59
RsaNI GTAC 1 cut(s) 58
RseI CAYNNNNRTG 1 cut(s) 913
Rsr2I CGGWCCG 1 cut(s) 334
RsrII CGGWCCG 1 cut(s) 334
SacI GAGCTC 1 cut(s) 383
SacII CCGCGG 1 cut(s) 531
Sau3AI GATC 3 cut(s) 52, 90, 549
Sau96I GGNCC 5 cut(s) 322, 334, 415, 558, 747
SchI GAGTC 3 cut(s) 623, 787, 967
SduI GDGCHC 1 cut(s) 383
SfaNI GCATC 2 cut(s) 405, 905
SfoI GGCGCC 2 cut(s) 454, 567
Sfr274I CTCGAG 1 cut(s) 382
Sfr303I CCGCGG 1 cut(s) 531
SgrAI CRCCGGYG 1 cut(s) 662
SgrBI CCGCGG 1 cut(s) 531
SinI GGWCC 3 cut(s) 334, 558, 747
SlaI CTCGAG 1 cut(s) 382
SmiMI CAYNNNNRTG 1 cut(s) 913
SmlI CTYRAG 1 cut(s) 382
SmoI CTYRAG 1 cut(s) 382
Sse9I AATT 3 cut(s) 118, 881, 922
SspDI GGCGCC 2 cut(s) 452, 565
SspMI CTAG 2 cut(s) 48, 1014
SstI GAGCTC 1 cut(s) 383
StyI CCWWGG 3 cut(s) 516, 720, 1013
TaaI ACNGT 4 cut(s) 558, 897, 933, 997
TaqI TCGA 3 cut(s) 123, 383, 447
TasI AATT 3 cut(s) 118, 881, 922
TfiI GAWTC 5 cut(s) 160, 358, 668, 784, 790
TseI GCWGC 5 cut(s) 214, 235, 694, 697, 802
TspDTI ATGAA 1 cut(s) 698
TspGWI ACGGA 1 cut(s) 45
VpaK11BI GGWCC 3 cut(s) 334, 558, 747
XagI CCTNNNNNAGG 1 cut(s) 634
XapI RAATTY 1 cut(s) 922
XcmI CCANNNNNNNNNTGG 1 cut(s) 1014
XhoI CTCGAG 1 cut(s) 382
XmaJI CCTAGG 1 cut(s) 1013
XmiI GTMKAC 1 cut(s) 264
XmnI GAANNNNTTC 1 cut(s) 284
XspI CTAG 2 cut(s) 48, 1014
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.