Rroxscaffold_3G00267780

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0011286
Physical Location & Seq
Forward (+)
60974433 .. 60975176
744 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00267780.1

Sequence Viewer

Length: 744 bp
ATGTGTAGGTCGACGGATTACCCCGGCTTTCAGGCCAGAAGAGAAGCCCTAAAGATTCAAGCTTTCTTTATACGGTTTTCGGGTTTGGATTTTCATAAGCCGTTGCCGGAATCACTCACTCTTTCGTATCTTCCGAGGATCAACGGCACCGCGCTCGAGATCGACGGGTCAAAGATCCGACCCGACTCGGCGGCGTTCGTGACGCTTCACCGCTCGGTTAATGCGAAGGTGAGGAAGGGGGAGGCGATATTCGGGTCGCGCGAGTCGGTTCGGGCCGGCGACGGGGTCCGGTTCGAGGCGTATTTGAGGGAGGAGAAGGTGCTGAAGGGGATTTTCAGGAAAGATGAGGGGCAGGAGTGGAAATTGGAGTGCAAGTGCGTGCTGGAGAGCGAGAGTGTCGGGGCGGAGGTCACGGCGGCGGAGGTGTGCGTGGCCGTGGAGGGACACGTGTCGATGAGCGAGAGGGTGGCGCTGATTGTGAAAAGGCGGGGAAAGTGTGGGTTTCATCCGCTGGAGGAGATTCCGGAGGTGGACGAGGAATCGGACGGCGGGTGTTGTTGCACTTGCGGGGAAATGGACGGTGGAGATTTGGAGGAGGATGGTGACGTGGAGGAGGAGTTGGAGGTGGATGTGGAGGGAGTGAGATGGGCTGTGGATGTGGGCTTTTGGGTCATGTGTTTGGGTGTGGGCTACATGGTTTCTAAGGCCTCATCCAAAAGCTTGAGACGCAGGAGATTATTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

247

Amino Acids

27.48

Weight (kDa)

5.19

Isoelectric Point (pI)

48.78

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 146
AccBSI CCGCTC 1 cut(s) 213
AccI GTMKAC 1 cut(s) 11
AccII CGCG 3 cut(s) 152, 259, 261
AccIII TCCGGA 1 cut(s) 523
AclWI GGATC 2 cut(s) 146, 169
AcoI YGGCCR 1 cut(s) 432
AcuI CTGAAG 1 cut(s) 344
AcvI CACGTG 1 cut(s) 448
AfiI CCNNNNNNNGG 2 cut(s) 282, 295
AflIII ACRYGT 2 cut(s) 445, 447
AgsI TTSAA 1 cut(s) 59
AjiI CACGTC 1 cut(s) 607
AluBI AGCT 2 cut(s) 62, 720
AluI AGCT 2 cut(s) 62, 720
Alw26I GTCTC 1 cut(s) 718
AlwI GGATC 2 cut(s) 146, 169
Ama87I CYCGRG 1 cut(s) 155
Aor13HI TCCGGA 1 cut(s) 523
AoxI GGCC 4 cut(s) 33, 273, 432, 705
AspLEI GCGC 3 cut(s) 154, 261, 472
AspS9I GGNCC 2 cut(s) 273, 286
AsuC2I CCSGG 1 cut(s) 24
AsuHPI GGTGA 3 cut(s) 200, 241, 614
AvaI CYCGRG 1 cut(s) 155
AvaII GGWCC 1 cut(s) 286
BanI GGYRCC 1 cut(s) 146
BbrPI CACGTG 1 cut(s) 448
BccI CCATC 2 cut(s) 593, 639
BceAI ACGGC 5 cut(s) 85, 160, 419, 429, 562
BcgI CGANNNNNNTGC 2 cut(s) 136, 170
BcnI CCSGG 1 cut(s) 24
BcoDI GTCTC 1 cut(s) 718
BfoI RGCGCY 1 cut(s) 473
BisI GCNGC 2 cut(s) 192, 417
BlsI GCNGC 2 cut(s) 193, 418
Bme1390I CCNGG 1 cut(s) 24
Bme18I GGWCC 1 cut(s) 286
BmeT110I CYCGRG 1 cut(s) 155
BmgBI CACGTC 1 cut(s) 607
BmgT120I GGNCC 2 cut(s) 273, 286
BmiI GGNNCC 2 cut(s) 148, 287
BmrFI CCNGG 1 cut(s) 24
BoxI GACNNNNGTC 1 cut(s) 448
BpmI CTGGAG 2 cut(s) 404, 533
BpuEI CTTGAG 1 cut(s) 742
BpuMI CCSGG 1 cut(s) 24
BsaAI YACGTR 1 cut(s) 448
BsaJI CCNNGG 3 cut(s) 22, 134, 435
BsaWI WCCGGW 2 cut(s) 288, 523
BsaXI ACNNNNNCTCC 4 cut(s) 359, 389, 602, 632
Bsc4I CCNNNNNNNGG 2 cut(s) 282, 295
Bse118I RCCGGY 1 cut(s) 275
BseAI TCCGGA 1 cut(s) 523
BseDI CCNNGG 3 cut(s) 22, 134, 435
BseGI GGATG 5 cut(s) 505, 604, 634, 661, 710
BseLI CCNNNNNNNGG 2 cut(s) 282, 295
BseRI GAGGAG 5 cut(s) 326, 530, 608, 626, 629
Bsh1236I CGCG 3 cut(s) 152, 259, 261
BshFI GGCC 4 cut(s) 35, 275, 434, 707
BshNI GGYRCC 1 cut(s) 146
BsiHKCI CYCGRG 1 cut(s) 155
BsiSI CCGG 5 cut(s) 24, 107, 276, 289, 524
BslFI GGGAC 1 cut(s) 456
BslI CCNNNNNNNGG 2 cut(s) 282, 295
BsmAI GTCTC 1 cut(s) 718
BsmBI CGTCTC 1 cut(s) 718
BsmFI GGGAC 1 cut(s) 456
BsnI GGCC 4 cut(s) 35, 275, 434, 707
BsoBI CYCGRG 1 cut(s) 155
Bsp13I TCCGGA 1 cut(s) 523
Bsp143I GATC 3 cut(s) 138, 159, 174
BspANI GGCC 4 cut(s) 35, 275, 434, 707
BspEI TCCGGA 1 cut(s) 523
BspFNI CGCG 3 cut(s) 152, 259, 261
BspLI GGNNCC 2 cut(s) 148, 287
BspPI GGATC 2 cut(s) 146, 169
BspT107I GGYRCC 1 cut(s) 146
BsrBI CCGCTC 1 cut(s) 213
BsrFI RCCGGY 1 cut(s) 275
BssAI RCCGGY 1 cut(s) 275
BssECI CCNNGG 3 cut(s) 22, 134, 435
BssMI GATC 3 cut(s) 138, 159, 174
Bst4CI ACNGT 2 cut(s) 75, 581
Bst6I CTCTTC 1 cut(s) 34
BstBAI YACGTR 1 cut(s) 448
BstC8I GCNNGC 2 cut(s) 277, 380
BstDEI CTNAG 1 cut(s) 702
BstDSI CCRYGG 1 cut(s) 435
BstF5I GGATG 5 cut(s) 505, 604, 634, 661, 710
BstFNI CGCG 3 cut(s) 152, 259, 261
BstH2I RGCGCY 1 cut(s) 473
BstHHI GCGC 3 cut(s) 154, 261, 472
BstKTI GATC 3 cut(s) 141, 162, 177
BstMAI GTCTC 1 cut(s) 718
BstMBI GATC 3 cut(s) 138, 159, 174
BstMWI GCNNNNNNNGC 1 cut(s) 726
BstPAI GACNNNNGTC 1 cut(s) 448
BstSCI CCNGG 1 cut(s) 22
BstUI CGCG 3 cut(s) 152, 259, 261
BstX2I RGATCY 1 cut(s) 174
BstYI RGATCY 1 cut(s) 174
BsuRI GGCC 4 cut(s) 35, 275, 434, 707
BtgI CCRYGG 1 cut(s) 435
BtrI CACGTC 1 cut(s) 607
BtsCI GGATG 5 cut(s) 505, 604, 634, 661, 710
Cac8I GCNNGC 2 cut(s) 277, 380
CfoI GCGC 3 cut(s) 154, 261, 472
Cfr10I RCCGGY 1 cut(s) 275
Cfr13I GGNCC 2 cut(s) 273, 286
CseI GACGC 2 cut(s) 211, 735
CviAII CATG 2 cut(s) 673, 694
DdeI CTNAG 1 cut(s) 702
DpnI GATC 3 cut(s) 140, 161, 176
DpnII GATC 3 cut(s) 138, 159, 174
EaeI YGGCCR 1 cut(s) 432
Eam1104I CTCTTC 1 cut(s) 34
EarI CTCTTC 1 cut(s) 34
EciI GGCGGA 2 cut(s) 419, 434
Eco147I AGGCCT 1 cut(s) 707
Eco47I GGWCC 1 cut(s) 286
Eco57I CTGAAG 1 cut(s) 344
Eco72I CACGTG 1 cut(s) 448
Eco88I CYCGRG 1 cut(s) 155
Esp3I CGTCTC 1 cut(s) 718
FaeI CATG 2 cut(s) 676, 697
FaiI YATR 4 cut(s) 71, 96, 674, 695
FaqI GGGAC 1 cut(s) 456
FatI CATG 2 cut(s) 672, 693
FauI CCCGC 3 cut(s) 480, 542, 560
FblI GTMKAC 1 cut(s) 11
Fnu4HI GCNGC 2 cut(s) 192, 417
FokI GGATG 5 cut(s) 492, 611, 641, 668, 697
Fsp4HI GCNGC 2 cut(s) 192, 417
GlaI GCGC 3 cut(s) 153, 260, 471
GluI GCNGC 2 cut(s) 192, 417
GsuI CTGGAG 2 cut(s) 404, 533
HaeII RGCGCY 1 cut(s) 473
HaeIII GGCC 4 cut(s) 35, 275, 434, 707
HapII CCGG 5 cut(s) 24, 107, 276, 289, 524
HgaI GACGC 2 cut(s) 211, 735
HhaI GCGC 3 cut(s) 154, 261, 472
Hin1II CATG 2 cut(s) 676, 697
Hin6I GCGC 3 cut(s) 152, 259, 470
HinP1I GCGC 3 cut(s) 152, 259, 470
HincII GTYRAC 1 cut(s) 12
HindII GTYRAC 1 cut(s) 12
HindIII AAGCTT 2 cut(s) 60, 718
HinfI GANTC 6 cut(s) 55, 110, 185, 263, 520, 539
HpaII CCGG 5 cut(s) 24, 107, 276, 289, 524
HphI GGTGA 3 cut(s) 200, 241, 614
Hpy166II GTNNAC 2 cut(s) 12, 532
Hpy188I TCNGA 3 cut(s) 135, 179, 544
Hpy188III TCNNGA 4 cut(s) 157, 199, 337, 524
Hpy8I GTNNAC 2 cut(s) 12, 532
Hpy99I CGWCG 3 cut(s) 16, 167, 284
HpyAV CCTTC 4 cut(s) 220, 229, 310, 319
HpyCH4III ACNGT 2 cut(s) 75, 581
HpyCH4IV ACGT 2 cut(s) 447, 606
HpyCH4V TGCA 2 cut(s) 372, 561
HpyF10VI GCNNNNNNNGC 1 cut(s) 726
HpyF3I CTNAG 1 cut(s) 702
HpySE526I ACGT 2 cut(s) 447, 606
Hsp92II CATG 2 cut(s) 676, 697
HspAI GCGC 3 cut(s) 152, 259, 470
Kpn2I TCCGGA 1 cut(s) 523
KroI GCCGGC 1 cut(s) 275
KroNI GCCGGC 1 cut(s) 277
Kzo9I GATC 3 cut(s) 138, 159, 174
MaeII ACGT 2 cut(s) 447, 606
MaeIII GTNAC 3 cut(s) 199, 409, 602
MalI GATC 3 cut(s) 140, 161, 176
MbiI CCGCTC 1 cut(s) 213
MboI GATC 3 cut(s) 138, 159, 174
MboII GAAGA 2 cut(s) 51, 122
MflI RGATCY 1 cut(s) 174
MluCI AATT 1 cut(s) 362
MlyI GAGTC 2 cut(s) 179, 272
MmeI TCCRAC 2 cut(s) 202, 600
MroI TCCGGA 1 cut(s) 523
MroNI GCCGGC 1 cut(s) 275
MseI TTAA 1 cut(s) 219
MspA1I CMGCKG 1 cut(s) 511
MspI CCGG 5 cut(s) 24, 107, 276, 289, 524
MspR9I CCNGG 1 cut(s) 24
MvnI CGCG 3 cut(s) 152, 259, 261
MwoI GCNNNNNNNGC 1 cut(s) 726
NaeI GCCGGC 1 cut(s) 277
NciI CCSGG 1 cut(s) 24
NdeII GATC 3 cut(s) 138, 159, 174
NgoMIV GCCGGC 1 cut(s) 275
NlaIII CATG 2 cut(s) 676, 697
NlaIV GGNNCC 2 cut(s) 148, 287
NmeAIII GCCGAG 1 cut(s) 167
NmuCI GTSAC 3 cut(s) 199, 409, 602
PaeR7I CTCGAG 1 cut(s) 155
PceI AGGCCT 1 cut(s) 707
PcsI WCGNNNNNNNCGW 3 cut(s) 131, 221, 258
PdiI GCCGGC 1 cut(s) 277
PfeI GAWTC 4 cut(s) 55, 110, 520, 539
PflFI GACNNNGTC 1 cut(s) 284
PkrI GCNGC 2 cut(s) 193, 418
PleI GAGTC 2 cut(s) 179, 271
PmaCI CACGTG 1 cut(s) 448
PmlI CACGTG 1 cut(s) 448
PpsI GAGTC 2 cut(s) 179, 271
Ppu21I YACGTR 1 cut(s) 448
PshAI GACNNNNGTC 1 cut(s) 448
PspCI CACGTG 1 cut(s) 448
PspN4I GGNNCC 2 cut(s) 148, 287
PspPI GGNCC 2 cut(s) 273, 286
PsuI RGATCY 1 cut(s) 174
PsyI GACNNNGTC 1 cut(s) 284
SalI GTCGAC 1 cut(s) 10
SaqAI TTAA 1 cut(s) 219
SatI GCNGC 2 cut(s) 192, 417
Sau3AI GATC 3 cut(s) 138, 159, 174
Sau96I GGNCC 2 cut(s) 273, 286
SchI GAGTC 2 cut(s) 179, 272
ScrFI CCNGG 1 cut(s) 24
Sfr274I CTCGAG 1 cut(s) 155
SinI GGWCC 1 cut(s) 286
SlaI CTCGAG 1 cut(s) 155
SmlI CTYRAG 2 cut(s) 155, 721
SmoI CTYRAG 2 cut(s) 155, 721
Sse9I AATT 1 cut(s) 362
SseBI AGGCCT 1 cut(s) 707
StuI AGGCCT 1 cut(s) 707
StyD4I CCNGG 1 cut(s) 22
TaaI ACNGT 2 cut(s) 75, 581
TaiI ACGT 2 cut(s) 450, 609
TaqI TCGA 5 cut(s) 11, 156, 162, 294, 452
TasI AATT 1 cut(s) 362
TauI GCSGC 2 cut(s) 194, 419
TfiI GAWTC 4 cut(s) 55, 110, 520, 539
Tru1I TTAA 1 cut(s) 219
Tru9I TTAA 1 cut(s) 219
TseFI GTSAC 3 cut(s) 199, 409, 602
Tsp45I GTSAC 3 cut(s) 199, 409, 602
TspDTI ATGAA 2 cut(s) 83, 494
TspGWI ACGGA 1 cut(s) 29
Tth111I GACNNNGTC 1 cut(s) 284
VpaK11BI GGWCC 1 cut(s) 286
XhoI CTCGAG 1 cut(s) 155
XmiI GTMKAC 1 cut(s) 11
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.