Rroxscaffold_5G00354500

Histone-lysine n-methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0002114
Physical Location & Seq
Forward (+)
32959600 .. 32973160
13561 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00354500.1

Sequence Viewer

Length: 3324 bp
ATGGCATTTCCCCTGAAGCATCGAAACGACGACGCCACCATCGACGTCGACACGGCCACGCCCCTCCGCTACCTCTCCCTCGACCACGTCTACTCCGCCACCTCCCCCTGCCGCGTCACCGCCAGCGGCTCCTCCAACGTCATGTCCAAGAAGGTCAAGGCTCGCAAGCTCAACCACTTTGAAAACGACGACGTTTCCGATCACAAGCCCCTTCCTCCTTCTCCTCCTCCTCCTCCGCCTCCGCCTCCGGCGCCGCCGCACAAGCCCGAGATTGTCCGTGTCTACTCGCGCCGCCGCGAGAAGAGGCCCCGCTCGTCGTCGTCCTTCTTCGACGGTCTCGTCGCTAGGGCACAGCCCAAGGCCGTCAAGGTCGAAGAGGTGGATGAGGATGACTATGTGAGATTGCTGGAGAAGAAGAAGAAGAAGAGGAGGAAGTTCGGGAGTAGCGAGCTAGTGAAATTGGGGGTTGATTCTGATGTTCTGCGTACATTGGACGCGCCTCGATTGAGAGAGTGTCGCAGCAGCAATCAGAAACTTGAGAAATCGAAATCCAGCTCTAAGAAGCGCAATTCTTCTGTTAACGCCGATAAGGTTCCACCTTCCGTGAAGAGATGGGTCGGGTTGAGTTTCACCGGAGTTGATCCGAAAACTTTTATTGGATTACAATGCAAGGCAAGCACTCTGTTTATTGGCCATTGGATGCTAATTCATATTCCGGTCGCGTCGTGGGATACAACTCTGACAGTTGAATATGAAGATGGTGATGAGGAAGATTTGATTCTTTCAAATGAGAGAGTTAAGTTTTATATTTCTCGGGATGAAATGGAGAGTTTGAATTTGAGTGCCAGTCTCAAGAATCTGGACAGTGATGTGTATGATTATAATGAGATGGTTGTGCTAGCAGCGAGTTTGGATGACTGTCAGGAACTAGAGCCTGGGGATATCATCTGGGCCAAGCTTACTGGCTATGCTATGTGGCCAGCAATTGTTGTGGATGAATCACTTATTGGTGACCGTGAAGGCTTAAGTAAAACTTTGGTTGGAGGATCAGTTCCAGTGCAATTCTTTGGTACCCATGACTTTGCAAGGATTAAAGTGAAGCAGGCTATCTCATTTCTCAAAGGACTTCTTTCTTCATTCCATTTGAAGTGCAGGAAGTCTCGATTTATTAAAAGCTTGGAAGAAGCGAAAATGTATCTCAGTGAACAAAAGCTTCCTAGAAGAATGCTACGATTGCAAAAAGGAATAAACGTTGATGATTGTGAAAGTGAAAATGGTGAGGATGGAATGCGCACTGATTCAGGAGAAGGATGTCTGGATGATGTCATGATTCCAAGGTCGCTGGACTGCCTTGGAACTTCTGTGTTAGTGATGGGGGATCTGCAGATAATAAGACTAGGCAAGGCATTGTACTATTGCACTGCTCCCACATTCCTAAATGCCACAATGCCAGGACTAGTAGATGAGAGATACGTTTGGCCTGAAGGTTATACTGCAGTAAGGAAGTTTACTTCAATAACAGATCCGAGTGTATGTACCATGTATAAGATGGAGGTACTGAGAGATCCTGAATCAAAAATTCGACCTCTGTTCCAAGTCTCAATAGACACTGGAGAGCAGTTCAAGGGATCGACACCGTCAGCTTGCTGGGATAAAGTATACAAAAGGATAAGGAAGATACAAAACAGTGCTCTGGATGATTCTGATGCTAATGCTGATGATGGATTAGAAAAGCTCTATAAATCTGGCTCTGAGATGTTTGGTTTCTCTAAACCTGAAGTTGCAAAACTTATACAGGGGTTATTAAAATCCAGCCATGCTTCAAACTTAGCCAAGTGCAAGTCAACCTCTAGAAGATATCGAGATGTTCCTGTTGGTTACAGACCAGTTCGTGTGGACTGGAAAGACCTTGATAAATGCAGTGTTTGCCACATGGATGAGAATGATGGTAAGCGTTTTCTTCAATTATTCAGATTTGGGGATCTCATAACTTCTGCACTGGCCAAGTATAGTTTTGTTTTGGTTGTACCCACTTTAGTAAACCAAGGTTCTTGGTCCATGCAAGATGCTATGGAGAATTGGAACCTGTTGGTGGTGTTCTATGGTTATGCAACTTATGCCGGCCTGGAGCTCCTGAACCTCCACCACCTTGCTGCCTTTGTCCTGGGTGCAATGAAGCCTACAACTGATGGGCGCTGGGCTCATTTGGCTTGCGCCATATGGATACCAGAAACTTGCCTATCTGATATCAAGAGAATGGAACCTATTGATGGGTTGAGCAGAATCAATAAGGATCGTTGGAAGCTGTTATGTAGTATCTGTGGTGTTTCTTATGGAGCCTGTATTCAATGTTCAAACCATACTTGTTATGCGGCATATCATCCACTTTGTGCACGAGCTGCTGGTCTTTGCGTTGAGAAGCATAAGCAGCCATCTAATGACCGTTCAGCGGCTGGTGACCGGATTGGCCGAACTGTGCGCCAATGTTCAGACTACACCCCACCATCTAATCCATCTGGATGTGCTCGTACTGATAAATTAAAACGTTTCTCCACTTTTGGTGAGCCATACAATTACTCCTGTAGAAGAGGGAGAAAAGAACCTGAAGCAATAGCTGCTGCATCCTTAAAGCGTTTGTTTGTTGAGAACCAACCTTACTTAGTTGGTGGGTACAGCCAACACCAATTGTCAAGCAATCCACTGCCTCCCAATTGTGTTGTTGGCTCTAAGTTCAGCTCGAGGCTTCTGAGGCTGAAAGCTTCTCAACTTGATGCTCCTACTGATATACTTTCTATGGCTGAGAAGTATAAGTACATGAGGGATACTTTTCGAAAAAGACTAGCGTTTGGAAAATCGGGAATTCATGGATTTGGTATATTTGCGAAGCACCCACATAGAGCAGGGGACATGGTGATTGAATACACTGGTGAACTTGTCAGACCTCCTATAGCTGACAGGAGAGAGCGCTTCATATATAATTCATTGGTGGGGGCTGGGACATATATGTTCCGGATTGATGATGAACGAGTCATTGATGCTACACGGGCTGGAAGCATTGCTCACCTGATCAATCATTCTTGTGAACCAAATTGCTACTCAAGAGTCATAAGTGTGAATTCTGACGAGCATATAATTATATTTGCAAAGAGAGACATTAAAAGATGGGAAGAACTTACATATGATTATAGGTTATCCTCGATTCTTTTTGTGCTTGCAGTTTCTACTTTTGTATCTCAGGATCAGGAGATGGATAGAAAGGTCCAAGACATAGTATCTGAGATTAGTATTGGAGATGAGTTGTTTAGGTTTCATTTAGAGTATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000785 GO:0001067 GO:0003002 GO:0003006 GO:0003674 GO:0003676 GO:0003677 GO:0003824 GO:0005488 GO:0005543 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005694 GO:0005737 GO:0005886 GO:0006325 GO:0006355 GO:0006464 GO:0006479 GO:0006807 GO:0006996 GO:0007275 GO:0007389 GO:0008150 GO:0008152 GO:0008168 GO:0008170 GO:0008213 GO:0008276 GO:0008289 GO:0008757 GO:0009653 GO:0009791 GO:0009889 GO:0009908 GO:0009909 GO:0009987 GO:0010093 GO:0010314 GO:0010468 GO:0010556 GO:0016020 GO:0016043 GO:0016278 GO:0016279 GO:0016569 GO:0016570 GO:0016571 GO:0016740 GO:0016741 GO:0018022 GO:0018024 GO:0018193 GO:0018205 GO:0019219 GO:0019222 GO:0019538 GO:0022414 GO:0031323 GO:0031326 GO:0032259 GO:0032501 GO:0032502 GO:0034968 GO:0035091 GO:0036211 GO:0042054 GO:0042800 GO:0043167 GO:0043168 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043412 GO:0043414 GO:0044212 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044427 GO:0044446 GO:0044464 GO:0048367 GO:0048437 GO:0048444 GO:0048449 GO:0048518 GO:0048578 GO:0048580 GO:0048582 GO:0048583 GO:0048584 GO:0048586 GO:0048608 GO:0048646 GO:0048731 GO:0048831 GO:0048856 GO:0050789 GO:0050793 GO:0050794 GO:0051094 GO:0051171 GO:0051239 GO:0051240 GO:0051252 GO:0051276 GO:0051568 GO:0060255 GO:0061458 GO:0065007 GO:0071704 GO:0071840 GO:0071944 GO:0080090 GO:0090567 GO:0090696 GO:0090697 GO:0090698 GO:0090701 GO:0097159 GO:0099402 GO:0140096 GO:1901363 GO:1901564 GO:1901981 GO:1903506 GO:1905392 GO:1905393 GO:2000026 GO:2000028 GO:2000112 GO:2000241 GO:2000243 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
FYRC FYRN PHD_2 PWWP SET zf-HC5HC2H_2

Protein Analysis

1107

Amino Acids

124.81

Weight (kDa)

8.73

Isoelectric Point (pI)

44.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PWWP PF00855 313 - 400 1.2e-15 PWWP domain
FYRN PF05964 457 - 521 1.8e-09 F/Y-rich N-terminus
FYRC PF05965 528 - 602 1.7e-11 F/Y rich C-terminus
zf-HC5HC2H_2 PF13832 723 - 811 1.7e-27 PHD-zinc-finger like domain
zf-HC5HC2H PF13771 735 - 810 3.9e-18 PHD-like zinc-binding domain
SET PF00856 956 - 1062 7.9e-25 SET domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 882
AasI GACNNNNNNGTC 1 cut(s) 338
AatII GACGTC 1 cut(s) 48
Acc16I TGCGCA 1 cut(s) 1292
Acc65I GGTACC 1 cut(s) 1070
AccB1I GGYRCC 2 cut(s) 250, 1070
AccBSI CCGCTC 1 cut(s) 312
AccI GTMKAC 4 cut(s) 48, 90, 282, 1659
AccII CGCG 5 cut(s) 114, 289, 297, 497, 722
AccIII TCCGGA 1 cut(s) 3009
AclI AACGTT 2 cut(s) 1251, 2545
AclWI GGATC 9 cut(s) 635, 1054, 1386, 1517, 1559, 1636, 1989, 2301, 3246
AcoI YGGCCR 5 cut(s) 54, 691, 977, 2001, 2467
AcsI RAATTY 4 cut(s) 835, 1578, 2859, 3115
AcuI CTGAAG 4 cut(s) 35, 1503, 1797, 2625
AcyI GRCGYC 3 cut(s) 33, 45, 251
AdeI CACNNNGTG 1 cut(s) 2390
AfaI GTAC 9 cut(s) 487, 1072, 1412, 1537, 1557, 2028, 2530, 2672, 2813
AfeI AGCGCT 1 cut(s) 2966
AfiI CCNNNNNNNGG 3 cut(s) 2092, 2270, 2449
AflII CTTAAG 1 cut(s) 1024
AhlI ACTAGT 1 cut(s) 1456
AjiI CACGTC 1 cut(s) 88
AjnI CCWGG 4 cut(s) 934, 1450, 2124, 2163
AjuI GAANNNNNNNTTGG 2 cut(s) 990, 1022
AloI GAACNNNNNNTCC 2 cut(s) 1035, 1067
Alw21I GWGCWC 4 cut(s) 1693, 2133, 2395, 2527
Alw26I GTCTC 5 cut(s) 341, 854, 1164, 1603, 3144
Alw44I GTGCAC 1 cut(s) 2391
AlwI GGATC 9 cut(s) 635, 1054, 1386, 1517, 1559, 1636, 1989, 2301, 3246
AlwNI CAGNNNCTG 1 cut(s) 2453
Ama87I CYCGRG 3 cut(s) 266, 813, 2737
Aor13HI TCCGGA 1 cut(s) 3009
Aor51HI AGCGCT 1 cut(s) 2966
ApaLI GTGCAC 1 cut(s) 2391
ApeKI GCWGC 8 cut(s) 519, 522, 902, 2153, 2399, 2428, 2615, 2618
ApoI RAATTY 4 cut(s) 835, 1578, 2859, 3115
Asp718I GGTACC 1 cut(s) 1070
AspLEI GCGC 9 cut(s) 253, 291, 499, 567, 1293, 2196, 2216, 2481, 2967
AspS9I GGNCC 4 cut(s) 306, 951, 2055, 3259
AsuII TTCGAA 1 cut(s) 2830
AsuNHI GCTAGC 1 cut(s) 898
AvaI CYCGRG 3 cut(s) 266, 813, 2737
AvaII GGWCC 2 cut(s) 2055, 3259
BaeGI GKGCMC 2 cut(s) 352, 2395
BaeI ACNNNNGTAYC 2 cut(s) 3213, 3246
BalI TGGCCA 3 cut(s) 693, 979, 2003
BanI GGYRCC 2 cut(s) 250, 1070
BanII GRGCYC 2 cut(s) 2133, 2203
BarI GAAGNNNNNNTAC 2 cut(s) 2795, 2827
BauI CACGAG 1 cut(s) 2394
Bbv12I GWGCWC 4 cut(s) 1693, 2133, 2395, 2527
BbvI GCAGC 8 cut(s) 531, 534, 914, 2140, 2386, 2440, 2602, 2605
BceAI ACGGC 2 cut(s) 69, 347
BciT130I CCWGG 4 cut(s) 936, 1452, 2126, 2165
BciVI GTATCC 3 cut(s) 724, 2217, 2815
BclI TGATCA 1 cut(s) 3066
BcoDI GTCTC 5 cut(s) 341, 854, 1164, 1603, 3144
BcuI ACTAGT 1 cut(s) 1456
BfaI CTAG 9 cut(s) 345, 452, 899, 929, 1218, 1397, 1457, 1851, 2840
BfmI CTRYAG 4 cut(s) 1382, 1494, 2581, 2946
BfoI RGCGCY 3 cut(s) 254, 2197, 2968
BfrI CTTAAG 1 cut(s) 1024
BfuI GTATCC 3 cut(s) 724, 2217, 2815
Bme1390I CCNGG 4 cut(s) 936, 1452, 2126, 2165
Bme18I GGWCC 2 cut(s) 2055, 3259
BmeT110I CYCGRG 3 cut(s) 266, 813, 2737
BmgBI CACGTC 1 cut(s) 88
BmgT120I GGNCC 4 cut(s) 306, 951, 2055, 3259
BmiI GGNNCC 8 cut(s) 130, 252, 308, 594, 1072, 2084, 2262, 2338
BmrFI CCNGG 4 cut(s) 936, 1452, 2126, 2165
BmsI GCATC 7 cut(s) 28, 690, 1696, 2056, 2630, 2761, 3025
BmtI GCTAGC 1 cut(s) 902
BpmI CTGGAG 3 cut(s) 428, 1632, 2147
Bpu14I TTCGAA 1 cut(s) 2830
BpuEI CTTGAG 3 cut(s) 557, 836, 3082
BsaHI GRCGYC 3 cut(s) 33, 45, 251
BsaI GGTCTC 1 cut(s) 341
BsaJI CCNNGG 6 cut(s) 357, 935, 1334, 1351, 2044, 2164
BsaWI WCCGGW 4 cut(s) 632, 715, 2460, 3009
BsaXI ACNNNNNCTCC 6 cut(s) 77, 107, 1035, 1065, 2561, 2591
Bsc4I CCNNNNNNNGG 3 cut(s) 2092, 2270, 2449
Bse118I RCCGGY 1 cut(s) 2120
Bse1I ACTGG 8 cut(s) 846, 967, 1055, 1615, 1886, 1904, 2004, 2929
Bse3DI GCAATG 2 cut(s) 2178, 3054
BseAI TCCGGA 1 cut(s) 3009
BseBI CCWGG 4 cut(s) 936, 1452, 2126, 2165
BseDI CCNNGG 6 cut(s) 357, 935, 1334, 1351, 2044, 2164
BseLI CCNNNNNNNGG 3 cut(s) 2092, 2270, 2449
BseMI GCAATG 2 cut(s) 2178, 3054
BseMII CTCAG 7 cut(s) 1213, 1550, 1743, 2738, 2790, 3248, 3267
BseNI ACTGG 8 cut(s) 846, 967, 1055, 1615, 1886, 1904, 2004, 2929
BseRI GAGGAG 6 cut(s) 121, 213, 216, 219, 222, 442
BseSI GKGCMC 2 cut(s) 352, 2395
BseXI GCAGC 8 cut(s) 531, 534, 914, 2140, 2386, 2440, 2602, 2605
BseYI CCCAGC 3 cut(s) 1647, 2196, 2993
BsgI GTGCAG 2 cut(s) 1171, 1980
Bsh1236I CGCG 5 cut(s) 114, 289, 297, 497, 722
Bsh1285I CGRYCG 1 cut(s) 720
BshNI GGYRCC 2 cut(s) 250, 1070
BsiEI CGRYCG 1 cut(s) 720
BsiHKAI GWGCWC 4 cut(s) 1693, 2133, 2395, 2527
BsiHKCI CYCGRG 3 cut(s) 266, 813, 2737
BsiSI CCGG 6 cut(s) 248, 633, 716, 2121, 2461, 3010
BslFI GGGAC 2 cut(s) 2918, 3010
BslI CCNNNNNNNGG 3 cut(s) 2092, 2270, 2449
BsmAI GTCTC 5 cut(s) 341, 854, 1164, 1603, 3144
BsmFI GGGAC 2 cut(s) 2918, 3010
BsmI GAATGC 2 cut(s) 1230, 1293
Bso31I GGTCTC 1 cut(s) 341
BsoBI CYCGRG 3 cut(s) 266, 813, 2737
Bsp119I TTCGAA 1 cut(s) 2830
Bsp1286I GDGCHC 6 cut(s) 352, 1693, 2133, 2203, 2395, 2527
Bsp13I TCCGGA 1 cut(s) 3009
BspCNI CTCAG 7 cut(s) 1212, 1551, 1744, 2739, 2791, 3247, 3268
BspEI TCCGGA 1 cut(s) 3009
BspFNI CGCG 5 cut(s) 114, 289, 297, 497, 722
BspHI TCATGA 1 cut(s) 1326
BspLI GGNNCC 8 cut(s) 130, 252, 308, 594, 1072, 2084, 2262, 2338
BspMAI CTGCAG 2 cut(s) 1386, 1498
BspOI GCTAGC 1 cut(s) 902
BspPI GGATC 9 cut(s) 635, 1054, 1386, 1517, 1559, 1636, 1989, 2301, 3246
BspT104I TTCGAA 1 cut(s) 2830
BspT107I GGYRCC 2 cut(s) 250, 1070
BspTI CTTAAG 1 cut(s) 1024
BspTNI GGTCTC 1 cut(s) 341
BsrBI CCGCTC 1 cut(s) 312
BsrDI GCAATG 2 cut(s) 2178, 3054
BsrFI RCCGGY 1 cut(s) 2120
BsrI ACTGG 8 cut(s) 846, 967, 1055, 1615, 1886, 1904, 2004, 2929
BssAI RCCGGY 1 cut(s) 2120
BssECI CCNNGG 6 cut(s) 357, 935, 1334, 1351, 2044, 2164
BssNAI GTATAC 1 cut(s) 1660
BssNI GRCGYC 3 cut(s) 33, 45, 251
BssSI CACGAG 1 cut(s) 2394
BssT1I CCWWGG 4 cut(s) 357, 1334, 1351, 2044
Bst1107I GTATAC 1 cut(s) 1660
Bst2BI CACGAG 1 cut(s) 2394
Bst2UI CCWGG 4 cut(s) 936, 1452, 2126, 2165
Bst4CI ACNGT 9 cut(s) 335, 745, 866, 920, 1016, 1638, 1688, 2444, 2476
Bst6I CTCTTC 5 cut(s) 296, 369, 419, 602, 2581
BstACI GRCGYC 3 cut(s) 33, 45, 251
BstAFI CTTAAG 1 cut(s) 1024
BstAPI GCANNNNNTGC 4 cut(s) 1926, 2117, 2399, 2615
BstBI TTCGAA 1 cut(s) 2830
BstEII GGTNACC 2 cut(s) 1010, 2456
BstFNI CGCG 5 cut(s) 114, 289, 297, 497, 722
BstH2I RGCGCY 3 cut(s) 254, 2197, 2968
BstHHI GCGC 9 cut(s) 253, 291, 499, 567, 1293, 2196, 2216, 2481, 2967
BstMAI GTCTC 5 cut(s) 341, 854, 1164, 1603, 3144
BstMCI CGRYCG 1 cut(s) 720
BstNI CCWGG 4 cut(s) 936, 1452, 2126, 2165
BstPI GGTNACC 2 cut(s) 1010, 2456
BstSCI CCNGG 4 cut(s) 934, 1450, 2124, 2163
BstSFI CTRYAG 4 cut(s) 1382, 1494, 2581, 2946
BstSLI GKGCMC 2 cut(s) 352, 2395
BstUI CGCG 5 cut(s) 114, 289, 297, 497, 722
BstV1I GCAGC 8 cut(s) 531, 534, 914, 2140, 2386, 2440, 2602, 2605
BstX2I RGATCY 4 cut(s) 1378, 1522, 1564, 1981
BstYI RGATCY 4 cut(s) 1378, 1522, 1564, 1981
BstZ17I GTATAC 1 cut(s) 1660
BsuI GTATCC 3 cut(s) 724, 2217, 2815
BtrI CACGTC 1 cut(s) 88
BtsI GCAGTG 3 cut(s) 1419, 1927, 2699
CaiI CAGNNNCTG 1 cut(s) 2453
CciI TCATGA 1 cut(s) 1326
CfoI GCGC 9 cut(s) 253, 291, 499, 567, 1293, 2196, 2216, 2481, 2967
Cfr10I RCCGGY 1 cut(s) 2120
Cfr13I GGNCC 4 cut(s) 306, 951, 2055, 3259
CseI GACGC 4 cut(s) 41, 103, 503, 711
Csp6I GTAC 9 cut(s) 486, 1071, 1411, 1536, 1556, 2027, 2529, 2671, 2812
CspCI CAANNNNNGTGG 2 cut(s) 972, 1007
CviQI GTAC 9 cut(s) 486, 1071, 1411, 1536, 1556, 2027, 2529, 2671, 2812
DinI GGCGCC 1 cut(s) 252
DraIII CACNNNGTG 1 cut(s) 2390
DrdI GACNNNNNNGTC 1 cut(s) 338
DseDI GACNNNNNNGTC 1 cut(s) 338
EaeI YGGCCR 5 cut(s) 54, 691, 977, 2001, 2467
Eam1104I CTCTTC 5 cut(s) 296, 369, 419, 602, 2581
EarI CTCTTC 5 cut(s) 296, 369, 419, 602, 2581
EciI GGCGGA 3 cut(s) 85, 225, 231
Ecl136II GAGCTC 1 cut(s) 2131
Eco130I CCWWGG 4 cut(s) 357, 1334, 1351, 2044
Eco24I GRGCYC 2 cut(s) 2133, 2203
Eco31I GGTCTC 1 cut(s) 341
Eco32I GATATC 3 cut(s) 943, 1859, 2248
Eco47I GGWCC 2 cut(s) 2055, 3259
Eco47III AGCGCT 1 cut(s) 2966
Eco53kI GAGCTC 1 cut(s) 2131
Eco57I CTGAAG 4 cut(s) 35, 1503, 1797, 2625
Eco88I CYCGRG 3 cut(s) 266, 813, 2737
Eco91I GGTNACC 2 cut(s) 1010, 2456
EcoICRI GAGCTC 1 cut(s) 2131
EcoO109I RGGNCCY 1 cut(s) 306
EcoO65I GGTNACC 2 cut(s) 1010, 2456
EcoRI GAATTC 2 cut(s) 2859, 3115
EcoRII CCWGG 4 cut(s) 934, 1450, 2124, 2163
EcoRV GATATC 3 cut(s) 943, 1859, 2248
EcoT14I CCWWGG 4 cut(s) 357, 1334, 1351, 2044
EcoT38I GRGCYC 2 cut(s) 2133, 2203
EgeI GGCGCC 1 cut(s) 252
EheI GGCGCC 1 cut(s) 252
ErhI CCWWGG 4 cut(s) 357, 1334, 1351, 2044
FalI AAGNNNNNCTT 4 cut(s) 1018, 1050, 1113, 1145
FaqI GGGAC 2 cut(s) 2918, 3010
FauI CCCGC 1 cut(s) 317
FauNDI CATATG 2 cut(s) 2219, 3178
FbaI TGATCA 1 cut(s) 3066
FblI GTMKAC 4 cut(s) 48, 90, 282, 1659
FriOI GRGCYC 2 cut(s) 2133, 2203
FspAI RTGCGCAY 1 cut(s) 1292
FspBI CTAG 9 cut(s) 345, 452, 899, 929, 1218, 1397, 1457, 1851, 2840
FspI TGCGCA 1 cut(s) 1292
GlaI GCGC 9 cut(s) 252, 290, 498, 566, 1292, 2195, 2215, 2480, 2966
GsaI CCCAGC 3 cut(s) 1651, 2200, 2997
GsuI CTGGAG 3 cut(s) 428, 1632, 2147
HaeII RGCGCY 3 cut(s) 254, 2197, 2968
HapII CCGG 6 cut(s) 248, 633, 716, 2121, 2461, 3010
HgaI GACGC 4 cut(s) 41, 103, 503, 711
HhaI GCGC 9 cut(s) 253, 291, 499, 567, 1293, 2196, 2216, 2481, 2967
Hin1I GRCGYC 3 cut(s) 33, 45, 251
Hin6I GCGC 9 cut(s) 251, 289, 497, 565, 1291, 2194, 2214, 2479, 2965
HinP1I GCGC 9 cut(s) 251, 289, 497, 565, 1291, 2194, 2214, 2479, 2965
HincII GTYRAC 3 cut(s) 49, 580, 1845
HindII GTYRAC 3 cut(s) 49, 580, 1845
HindIII AAGCTT 4 cut(s) 956, 1174, 1211, 2757
HpaI GTTAAC 1 cut(s) 580
HpaII CCGG 6 cut(s) 248, 633, 716, 2121, 2461, 3010
HpyAV CCTTC 8 cut(s) 145, 221, 228, 334, 609, 1013, 1301, 1478
HpyCH4III ACNGT 9 cut(s) 335, 745, 866, 920, 1016, 1638, 1688, 2444, 2476
HpyCH4IV ACGT 7 cut(s) 45, 87, 138, 192, 1251, 1473, 2545
HpySE526I ACGT 7 cut(s) 45, 87, 138, 192, 1251, 1473, 2545
Hsp92I GRCGYC 3 cut(s) 33, 45, 251
HspAI GCGC 9 cut(s) 251, 289, 497, 565, 1291, 2194, 2214, 2479, 2965
KasI GGCGCC 1 cut(s) 250
Kpn2I TCCGGA 1 cut(s) 3009
KpnI GGTACC 1 cut(s) 1074
KroI GCCGGC 1 cut(s) 2120
KroNI GCCGGC 1 cut(s) 2122
Ksp22I TGATCA 1 cut(s) 3066
KspAI GTTAAC 1 cut(s) 580
LmnI GCTCC 6 cut(s) 134, 1429, 2128, 2136, 2336, 2779
Lsp1109I GCAGC 8 cut(s) 531, 534, 914, 2140, 2386, 2440, 2602, 2605
LweI GCATC 7 cut(s) 28, 690, 1696, 2056, 2630, 2761, 3025
MaeI CTAG 9 cut(s) 345, 452, 899, 929, 1218, 1397, 1457, 1851, 2840
MaeII ACGT 7 cut(s) 45, 87, 138, 192, 1251, 1473, 2545
MaeIII GTNAC 4 cut(s) 115, 1010, 1877, 2456
MbiI CCGCTC 1 cut(s) 312
MfeI CAATTG 3 cut(s) 984, 2684, 2710
MflI RGATCY 4 cut(s) 1378, 1522, 1564, 1981
MhlI GDGCHC 6 cut(s) 352, 1693, 2133, 2203, 2395, 2527
MlsI TGGCCA 3 cut(s) 693, 979, 2003
MluNI TGGCCA 3 cut(s) 693, 979, 2003
Mly113I GGCGCC 1 cut(s) 251
MlyI GAGTC 2 cut(s) 3036, 3111
MmeI TCCRAC 3 cut(s) 159, 1021, 2279
Mox20I TGGCCA 3 cut(s) 693, 979, 2003
MroI TCCGGA 1 cut(s) 3009
MroNI GCCGGC 1 cut(s) 2120
MscI TGGCCA 3 cut(s) 693, 979, 2003
MseI TTAA 9 cut(s) 579, 798, 1025, 1092, 1170, 1805, 2540, 2627, 3156
MslI CAYNNNNRTG 4 cut(s) 1935, 2518, 3078, 3110
Msp20I TGGCCA 3 cut(s) 693, 979, 2003
MspA1I CMGCKG 2 cut(s) 126, 2450
MspCI CTTAAG 1 cut(s) 1024
MspI CCGG 6 cut(s) 248, 633, 716, 2121, 2461, 3010
MspR9I CCNGG 4 cut(s) 936, 1452, 2126, 2165
MunI CAATTG 3 cut(s) 984, 2684, 2710
Mva1269I GAATGC 2 cut(s) 1230, 1293
MvaI CCWGG 4 cut(s) 936, 1452, 2126, 2165
MvnI CGCG 5 cut(s) 114, 289, 297, 497, 722
NaeI GCCGGC 1 cut(s) 2122
NarI GGCGCC 1 cut(s) 251
NdeI CATATG 2 cut(s) 2219, 3178
NgoMIV GCCGGC 1 cut(s) 2120
NheI GCTAGC 1 cut(s) 898
NlaIV GGNNCC 8 cut(s) 130, 252, 308, 594, 1072, 2084, 2262, 2338
NmuCI GTSAC 3 cut(s) 115, 1010, 2456
NsbI TGCGCA 1 cut(s) 1292
NspV TTCGAA 1 cut(s) 2830
PaeR7I CTCGAG 1 cut(s) 2737
PagI TCATGA 1 cut(s) 1326
PcsI WCGNNNNNNNCGW 4 cut(s) 39, 195, 336, 444
PctI GAATGC 2 cut(s) 1230, 1293
PdiI GCCGGC 1 cut(s) 2122
PflFI GACNNNGTC 2 cut(s) 86, 1636
PleI GAGTC 2 cut(s) 3035, 3110
PluTI GGCGCC 1 cut(s) 254
PpsI GAGTC 2 cut(s) 3035, 3110
PsiI TTATAA 1 cut(s) 882
Psp124BI GAGCTC 1 cut(s) 2133
Psp1406I AACGTT 2 cut(s) 1251, 2545
Psp6I CCWGG 4 cut(s) 934, 1450, 2124, 2163
PspEI GGTNACC 2 cut(s) 1010, 2456
PspFI CCCAGC 3 cut(s) 1647, 2196, 2993
PspGI CCWGG 4 cut(s) 934, 1450, 2124, 2163
PspN4I GGNNCC 8 cut(s) 130, 252, 308, 594, 1072, 2084, 2262, 2338
PspPI GGNCC 4 cut(s) 306, 951, 2055, 3259
PspXI VCTCGAGB 1 cut(s) 2737
PsrI GAACNNNNNNTAC 4 cut(s) 2335, 2367, 2639, 2671
PstI CTGCAG 2 cut(s) 1386, 1498
PstNI CAGNNNCTG 1 cut(s) 2453
PsuI RGATCY 4 cut(s) 1378, 1522, 1564, 1981
PsyI GACNNNGTC 2 cut(s) 86, 1636
RsaI GTAC 9 cut(s) 487, 1072, 1412, 1537, 1557, 2028, 2530, 2672, 2813
RsaNI GTAC 9 cut(s) 486, 1071, 1411, 1536, 1556, 2027, 2529, 2671, 2812
RseI CAYNNNNRTG 4 cut(s) 1935, 2518, 3078, 3110
SacI GAGCTC 1 cut(s) 2133
SalI GTCGAC 1 cut(s) 47
SaqAI TTAA 9 cut(s) 579, 798, 1025, 1092, 1170, 1805, 2540, 2627, 3156
Sau96I GGNCC 4 cut(s) 306, 951, 2055, 3259
SchI GAGTC 2 cut(s) 3036, 3111
ScrFI CCNGG 4 cut(s) 936, 1452, 2126, 2165
SduI GDGCHC 6 cut(s) 352, 1693, 2133, 2203, 2395, 2527
SfaNI GCATC 7 cut(s) 28, 690, 1696, 2056, 2630, 2761, 3025
SfcI CTRYAG 4 cut(s) 1382, 1494, 2581, 2946
SfoI GGCGCC 1 cut(s) 252
Sfr274I CTCGAG 1 cut(s) 2737
SfuI TTCGAA 1 cut(s) 2830
SinI GGWCC 2 cut(s) 2055, 3259
SlaI CTCGAG 1 cut(s) 2737
SmiMI CAYNNNNRTG 4 cut(s) 1935, 2518, 3078, 3110
SmlI CTYRAG 5 cut(s) 536, 851, 1024, 2737, 3097
SmoI CTYRAG 5 cut(s) 536, 851, 1024, 2737, 3097
SpeI ACTAGT 1 cut(s) 1456
SspDI GGCGCC 1 cut(s) 250
SspMI CTAG 9 cut(s) 345, 452, 899, 929, 1218, 1397, 1457, 1851, 2840
SstI GAGCTC 1 cut(s) 2133
StyD4I CCNGG 4 cut(s) 934, 1450, 2124, 2163
StyI CCWWGG 4 cut(s) 357, 1334, 1351, 2044
TaaI ACNGT 9 cut(s) 335, 745, 866, 920, 1016, 1638, 1688, 2444, 2476
TaiI ACGT 7 cut(s) 48, 90, 141, 195, 1254, 1476, 2548
TatI WGTACW 2 cut(s) 1410, 2811
TauI GCSGC 8 cut(s) 114, 129, 256, 259, 294, 297, 2375, 2453
Tru1I TTAA 9 cut(s) 579, 798, 1025, 1092, 1170, 1805, 2540, 2627, 3156
Tru9I TTAA 9 cut(s) 579, 798, 1025, 1092, 1170, 1805, 2540, 2627, 3156
TseFI GTSAC 3 cut(s) 115, 1010, 2456
TseI GCWGC 8 cut(s) 519, 522, 902, 2153, 2399, 2428, 2615, 2618
Tsp45I GTSAC 3 cut(s) 115, 1010, 2456
TspGWI ACGGA 2 cut(s) 266, 592
Tth111I GACNNNGTC 2 cut(s) 86, 1636
Vha464I CTTAAG 1 cut(s) 1024
VneI GTGCAC 1 cut(s) 2391
VpaK11BI GGWCC 2 cut(s) 2055, 3259
XapI RAATTY 4 cut(s) 835, 1578, 2859, 3115
XbaI TCTAGA 1 cut(s) 1850
XcmI CCANNNNNNNNNTGG 1 cut(s) 1546
XhoI CTCGAG 1 cut(s) 2737
XmiI GTMKAC 4 cut(s) 48, 90, 282, 1659
XspI CTAG 9 cut(s) 345, 452, 899, 929, 1218, 1397, 1457, 1851, 2840
ZraI GACGTC 1 cut(s) 46
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.