Rroxscaffold_6G00430180

FRIGIDA-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0005538
Physical Location & Seq
Forward (+)
50373123 .. 50376507
3385 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00430180.1

Sequence Viewer

Length: 2112 bp
ATGGAGAAGACGACTGTGGATTCGAAGCTGAAAGAATACGAGACCAGTTTCCGCAAAAAGTTCGAGACATTTCAATCCCAGGCGGCCTCCCTCGTCCACCTGACCAAGCAATACAAGGAGCTCCAGGCCCGGTTCGACTCCGCCCAGAACGACCTCCGGACCCGGTTCCGGGAGCTCAAGGCCCGGGAGAACCAAATCGGAGTCAAAGAGAAGGAGGCCGAACAGTGGAGTAAGAGGCAACTGCTTTCTTTGAAATCTTTGATTAAGAAACACGTTGATGAGCTTGATGTGTTGAGTGAGAGGGTTAGGGAGAAGAGGGAGGTGTGTGAGGTGACCCAAAGCCTGGTTTCGGAGAGGAAGAATGAGTTGAGTTGGGTTGAGAGATGTGTGTTTGACAAGAGGAGAGAGGTTGAGGAGTGTAAGAGAGAGTGTGATCTGAAACAAAGGTGCATTGATGAGTGTGAGAAAGAGATGGAATTGAAAAGGGAGATGATAGAGGACATGGATTTGAGAGCGAAAGAGTCAATGGAGGAGTGGCGTTGTAGGCTTGAATTGAAAGAGAGGGAGCTGGAAGGGTTGTTTGACAAGTTTGAATTGAGAGGGCAAGAGTTTGAGCCAAATGTTGAGGAGATTCGTGTTATTGATAAGAGGGTGGAGGAGTGCATCAATGAGGTTGAGATTAAGGAGAGGTGTTTGGAGAGACTTGAGAAATCAATAGAGGAACGCGATAAGTGTTTGGATTCAGTCTCCCACGAAATGAAGATGAAAGAGGGGCAACTTGAAGAGCAGGCTAAAGAGTTTGAGTTGTTTGCTTCACAAGTGAAGACTGAGCAGTTGGAAAAAGAAAAGCATTTTGCTTCACTTGAAAAAATGATGGAAGAACGAGAGAAGACCTTGGATGCACTTGAAAAATCAATACAAGGGCGTGAAAAGTCTTTGGATTCAGTCTCCCATGAACTGAAGATGAAAGAGGAGCAGCTTGAAGAGCAAGCCAAAGAGTTTAAACTGAAGCAAATTGCATTTGCTTCCCAAGTGAAGACTGAAAAGTTGGAAAGAGAAGAGCATTTTGCTTCACTTGAAAAATCGATCCAACAAAGAGAAAAGACTTTGGATTCACTCAAGAAGTCGTTCCAAGAGAGCAAAGAGCATTTGGAGAGACAACTTGAAGAGCAGGGCAGAGAGCTTCAATCAAAGCAAAAACTATTCGATTCTCAGCAATTGGAAAGAGAAAAGCAACTTGATATATTTGAAAAGTCCTTACAAAAACGTGAAAAGCAGTTCAATGTACTCCAAAAGTCAGTTCAAGAGCAGAAGGAGCATTTGGATTCACGCTCCCATAGACTCCAGATGGAAAAGAGGCAACTTGAAAAGCAGGGCAAAGAGCTTGAATTGAAGCTAAAACATCGTGAATCTCAAGTGAAGGCTGAGCAGTTGGAACTTACACCCACTGCTCATAATATGATTATTCCATCATTTATAAGTGATCAACCCAACATCAGTACAGATGGTAGAAGCTTGCAGTTGTCAATCGCTGAGAATTTAAACAAAGCTGAATTGGTGGGTGGCGAATTGTCAGCTCTTTTAGATGCAGATGAGATTTTAAAGCTTCTTGGAATGATTTCTCAGCATAAGCAGACTTTACAGTTATGTCAGACACTTGGTTTTGAAGATAAAATCCCTAATATTATTTGGAATCTCATTGAAAGAAAGCAACTGATTGAGGCTGTTAGATTTATCTGCACCTTCAAGCTAAATGACGAGTTTCCCCCAGCACCACTCTTGATAGAATTTGTGGAGGATGCAAAGAAGTGTTGCAGTGAAATGCTCAGTGAAGTGAAATCACATGATGAAAAGGAAAAGTTTGTGGACGACCGAATAGCTGATCTAAGAGCTGTGGTTCAATGCATTGAAGACCACGGCCTCAAGTCCCAATACTACTCAATCGACATTTTGATGGATATTCAAGGTCTTCAGAAACTAAAGGAGAATGGAAAATTGGTGCTGTCTCTTCCACCCAGTGTCGAAAAACAAGAGCAGAGAAAAGGGAGGAAACAAAGTTCCAGCAGTCTCCCCTCCACACCTCACACATATCAGCTGCGTAAAAGAACATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04530 map04530
Pfam Domains
Protein Families
Frigida

Protein Analysis

703

Amino Acids

83.21

Weight (kDa)

5.72

Isoelectric Point (pI)

54.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Frigida PF07899 526 - 664 3.4e-32 Frigida-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1478
AccB7I CCANNNNNTGG 1 cut(s) 343
AccII CGCG 1 cut(s) 726
AccIII TCCGGA 1 cut(s) 156
AciI CCGC 3 cut(s) 52, 83, 141
AclWI GGATC 1 cut(s) 1081
AcsI RAATTY 2 cut(s) 1537, 1787
AcuI CTGAAG 3 cut(s) 980, 1028, 1955
AdeI CACNNNGTG 1 cut(s) 2018
AfaI GTAC 2 cut(s) 1287, 1501
AfiI CCNNNNNNNGG 5 cut(s) 168, 169, 225, 343, 349
AflIII ACRYGT 1 cut(s) 271
AjnI CCWGG 3 cut(s) 78, 123, 342
AjuI GAANNNNNNNTTGG 6 cut(s) 1284, 1304, 1316, 1336, 1979, 2011
AloI GAACNNNNNNTCC 2 cut(s) 2041, 2073
Alw21I GWGCWC 2 cut(s) 123, 177
Alw26I GTCTC 8 cut(s) 35, 59, 694, 751, 952, 1150, 2010, 2072
AlwI GGATC 1 cut(s) 1081
Ama87I CYCGRG 1 cut(s) 183
Aor13HI TCCGGA 1 cut(s) 156
AoxI GGCC 5 cut(s) 84, 126, 180, 216, 1918
ApeKI GCWGC 2 cut(s) 976, 2095
ApoI RAATTY 2 cut(s) 1537, 1787
ArsI GACNNNNNNTTYG 1 cut(s) 36
Asp700I GAANNNNTTC 2 cut(s) 1127, 1618
AspS9I GGNCC 3 cut(s) 127, 159, 181
AsuC2I CCSGG 5 cut(s) 130, 163, 170, 184, 185
AsuHPI GGTGA 1 cut(s) 343
AsuII TTCGAA 1 cut(s) 23
AvaI CYCGRG 1 cut(s) 183
AvaII GGWCC 1 cut(s) 159
BanII GRGCYC 2 cut(s) 123, 177
BbsI GAAGAC 6 cut(s) 14, 830, 896, 1043, 1917, 1961
Bbv12I GWGCWC 2 cut(s) 123, 177
BbvI GCAGC 2 cut(s) 988, 2082
BccI CCATC 6 cut(s) 466, 868, 1342, 1477, 1499, 1948
BceAI ACGGC 1 cut(s) 1933
BcgI CGANNNNNNTGC 2 cut(s) 43, 77
BciT130I CCWGG 3 cut(s) 80, 125, 344
BclI TGATCA 1 cut(s) 1483
BcnI CCSGG 5 cut(s) 130, 163, 170, 184, 185
BcoDI GTCTC 8 cut(s) 35, 59, 694, 751, 952, 1150, 2010, 2072
BisI GCNGC 3 cut(s) 84, 977, 2096
BlpI GCTNAGC 1 cut(s) 1425
BlsI GCNGC 3 cut(s) 85, 978, 2097
Bme1390I CCNGG 8 cut(s) 80, 125, 130, 163, 170, 184, 185, 344
Bme18I GGWCC 1 cut(s) 159
BmeT110I CYCGRG 1 cut(s) 183
BmgT120I GGNCC 3 cut(s) 127, 159, 181
BmiI GGNNCC 2 cut(s) 161, 167
BmrFI CCNGG 8 cut(s) 80, 125, 130, 163, 170, 184, 185, 344
BmrI ACTGGG 1 cut(s) 2010
BmsI GCATC 4 cut(s) 672, 889, 1576, 1789
BmuI ACTGGG 1 cut(s) 2010
BpiI GAAGAC 6 cut(s) 14, 830, 896, 1043, 1917, 1961
BpmI CTGGAG 2 cut(s) 107, 1328
Bpu1102I GCTNAGC 1 cut(s) 1425
Bpu14I TTCGAA 1 cut(s) 23
BpuEI CTTGAG 5 cut(s) 161, 725, 1103, 1398, 1907
BpuMI CCSGG 5 cut(s) 130, 163, 170, 184, 185
Bsa29I ATCGAT 1 cut(s) 1085
BsaI GGTCTC 1 cut(s) 35
BsaJI CCNNGG 4 cut(s) 78, 183, 894, 1915
BsaWI WCCGGW 1 cut(s) 156
Bsc4I CCNNNNNNNGG 5 cut(s) 168, 169, 225, 343, 349
Bse1I ACTGG 2 cut(s) 45, 2016
BseAI TCCGGA 1 cut(s) 156
BseBI CCWGG 3 cut(s) 80, 125, 344
BseCI ATCGAT 1 cut(s) 1085
BseDI CCNNGG 4 cut(s) 78, 183, 894, 1915
BseGI GGATG 2 cut(s) 904, 1804
BseLI CCNNNNNNNGG 5 cut(s) 168, 169, 225, 343, 349
BseMII CTCAG 6 cut(s) 819, 1226, 1416, 1524, 1637, 1840
BseNI ACTGG 2 cut(s) 45, 2016
BseRI GAGGAG 6 cut(s) 415, 428, 545, 641, 671, 986
BseXI GCAGC 2 cut(s) 988, 2082
BseYI CCCAGC 1 cut(s) 1768
BsgI GTGCAG 1 cut(s) 1723
Bsh1236I CGCG 1 cut(s) 726
Bsh1285I CGRYCG 1 cut(s) 1873
BshFI GGCC 5 cut(s) 86, 128, 182, 218, 1920
BshVI ATCGAT 1 cut(s) 1085
BsiEI CGRYCG 1 cut(s) 1873
BsiHKAI GWGCWC 2 cut(s) 123, 177
BsiHKCI CYCGRG 1 cut(s) 183
BsiSI CCGG 5 cut(s) 130, 157, 163, 169, 184
BslFI GGGAC 1 cut(s) 1912
BslI CCNNNNNNNGG 5 cut(s) 168, 169, 225, 343, 349
BsmAI GTCTC 8 cut(s) 35, 59, 694, 751, 952, 1150, 2010, 2072
BsmFI GGGAC 1 cut(s) 1912
BsnI GGCC 5 cut(s) 86, 128, 182, 218, 1920
Bso31I GGTCTC 1 cut(s) 35
BsoBI CYCGRG 1 cut(s) 183
Bsp119I TTCGAA 1 cut(s) 23
Bsp1286I GDGCHC 2 cut(s) 123, 177
Bsp13I TCCGGA 1 cut(s) 156
Bsp143I GATC 4 cut(s) 433, 1086, 1483, 1882
Bsp1720I GCTNAGC 1 cut(s) 1425
BspACI CCGC 3 cut(s) 52, 83, 141
BspANI GGCC 5 cut(s) 86, 128, 182, 218, 1920
BspCNI CTCAG 6 cut(s) 820, 1225, 1417, 1525, 1636, 1839
BspDI ATCGAT 1 cut(s) 1085
BspEI TCCGGA 1 cut(s) 156
BspFNI CGCG 1 cut(s) 726
BspLI GGNNCC 2 cut(s) 161, 167
BspPI GGATC 1 cut(s) 1081
BspQI GCTCTTC 4 cut(s) 777, 978, 1053, 1161
BspT104I TTCGAA 1 cut(s) 23
BspTNI GGTCTC 1 cut(s) 35
BsrI ACTGG 2 cut(s) 45, 2016
BssECI CCNNGG 4 cut(s) 78, 183, 894, 1915
BssMI GATC 4 cut(s) 433, 1086, 1483, 1882
BssT1I CCWWGG 1 cut(s) 894
Bst2UI CCWGG 3 cut(s) 80, 125, 344
Bst4CI ACNGT 3 cut(s) 16, 225, 1644
Bst6I CTCTTC 6 cut(s) 308, 777, 978, 1053, 1161, 2013
BstBI TTCGAA 1 cut(s) 23
BstC8I GCNNGC 3 cut(s) 789, 990, 1517
BstDEI CTNAG 7 cut(s) 828, 1212, 1425, 1533, 1623, 1826, 1886
BstDSI CCRYGG 1 cut(s) 1915
BstEII GGTNACC 1 cut(s) 331
BstF5I GGATG 2 cut(s) 904, 1804
BstFNI CGCG 1 cut(s) 726
BstKTI GATC 4 cut(s) 436, 1089, 1486, 1885
BstMAI GTCTC 8 cut(s) 35, 59, 694, 751, 952, 1150, 2010, 2072
BstMBI GATC 4 cut(s) 433, 1086, 1483, 1882
BstMCI CGRYCG 1 cut(s) 1873
BstMWI GCNNNNNNNGC 3 cut(s) 544, 985, 1315
BstNI CCWGG 3 cut(s) 80, 125, 344
BstPI GGTNACC 1 cut(s) 331
BstSCI CCNGG 8 cut(s) 78, 123, 128, 161, 168, 182, 183, 342
BstUI CGCG 1 cut(s) 726
BstV1I GCAGC 2 cut(s) 988, 2082
BstV2I GAAGAC 6 cut(s) 14, 830, 896, 1043, 1917, 1961
Bsu15I ATCGAT 1 cut(s) 1085
BsuRI GGCC 5 cut(s) 86, 128, 182, 218, 1920
BsuTUI ATCGAT 1 cut(s) 1085
BtgI CCRYGG 1 cut(s) 1915
BtsCI GGATG 2 cut(s) 904, 1804
BtsI GCAGTG 2 cut(s) 1446, 1822
BtsIMutI CAGTG 5 cut(s) 230, 1446, 1822, 1834, 2023
Cac8I GCNNGC 3 cut(s) 789, 990, 1517
Cfr13I GGNCC 3 cut(s) 127, 159, 181
Cfr9I CCCGGG 1 cut(s) 183
ClaI ATCGAT 1 cut(s) 1085
Csp6I GTAC 2 cut(s) 1286, 1500
CviAII CATG 3 cut(s) 502, 953, 1844
CviQI GTAC 2 cut(s) 1286, 1500
DdeI CTNAG 7 cut(s) 828, 1212, 1425, 1533, 1623, 1826, 1886
DpnI GATC 4 cut(s) 435, 1088, 1485, 1884
DpnII GATC 4 cut(s) 433, 1086, 1483, 1882
DraI TTTAAA 3 cut(s) 1003, 1542, 1602
DraIII CACNNNGTG 1 cut(s) 2018
Eam1104I CTCTTC 6 cut(s) 308, 777, 978, 1053, 1161, 2013
EarI CTCTTC 6 cut(s) 308, 777, 978, 1053, 1161, 2013
EciI GGCGGA 1 cut(s) 130
Ecl136II GAGCTC 2 cut(s) 121, 175
Eco130I CCWWGG 1 cut(s) 894
Eco24I GRGCYC 2 cut(s) 123, 177
Eco31I GGTCTC 1 cut(s) 35
Eco47I GGWCC 1 cut(s) 159
Eco53kI GAGCTC 2 cut(s) 121, 175
Eco57I CTGAAG 3 cut(s) 980, 1028, 1955
Eco88I CYCGRG 1 cut(s) 183
Eco91I GGTNACC 1 cut(s) 331
EcoICRI GAGCTC 2 cut(s) 121, 175
EcoO65I GGTNACC 1 cut(s) 331
EcoRII CCWGG 3 cut(s) 78, 123, 342
EcoT14I CCWWGG 1 cut(s) 894
EcoT22I ATGCAT 1 cut(s) 1907
EcoT38I GRGCYC 2 cut(s) 123, 177
ErhI CCWWGG 1 cut(s) 894
FaeI CATG 3 cut(s) 505, 956, 1847
FaqI GGGAC 1 cut(s) 1912
FatI CATG 3 cut(s) 501, 952, 1843
FbaI TGATCA 1 cut(s) 1483
Fnu4HI GCNGC 3 cut(s) 84, 977, 2096
FokI GGATG 2 cut(s) 911, 1811
FriOI GRGCYC 2 cut(s) 123, 177
Fsp4HI GCNGC 3 cut(s) 84, 977, 2096
GluI GCNGC 3 cut(s) 84, 977, 2096
GsaI CCCAGC 1 cut(s) 1772
GsuI CTGGAG 2 cut(s) 107, 1328
HaeIII GGCC 5 cut(s) 86, 128, 182, 218, 1920
HapII CCGG 5 cut(s) 130, 157, 163, 169, 184
Hin1II CATG 3 cut(s) 505, 956, 1847
HindIII AAGCTT 2 cut(s) 1513, 1604
HpaII CCGG 5 cut(s) 130, 157, 163, 169, 184
HphI GGTGA 1 cut(s) 343
Hpy166II GTNNAC 2 cut(s) 97, 1867
Hpy188I TCNGA 5 cut(s) 200, 352, 438, 1653, 1974
Hpy188III TCNNGA 7 cut(s) 64, 157, 1120, 1304, 1345, 1406, 1780
Hpy8I GTNNAC 2 cut(s) 97, 1867
HpyAV CCTTC 5 cut(s) 205, 566, 1306, 1414, 1753
HpyCH4III ACNGT 3 cut(s) 16, 225, 1644
HpyCH4IV ACGT 2 cut(s) 273, 1267
HpyF10VI GCNNNNNNNGC 3 cut(s) 544, 985, 1315
HpyF3I CTNAG 7 cut(s) 828, 1212, 1425, 1533, 1623, 1826, 1886
HpySE526I ACGT 2 cut(s) 273, 1267
Hsp92II CATG 3 cut(s) 505, 956, 1847
Kpn2I TCCGGA 1 cut(s) 156
Ksp22I TGATCA 1 cut(s) 1483
Kzo9I GATC 4 cut(s) 433, 1086, 1483, 1882
LguI GCTCTTC 4 cut(s) 777, 978, 1053, 1161
LmnI GCTCC 7 cut(s) 118, 126, 172, 565, 973, 1315, 1337
Lsp1109I GCAGC 2 cut(s) 988, 2082
LweI GCATC 4 cut(s) 672, 889, 1576, 1789
MaeII ACGT 2 cut(s) 273, 1267
MaeIII GTNAC 1 cut(s) 331
MalI GATC 4 cut(s) 435, 1088, 1485, 1884
MboI GATC 4 cut(s) 433, 1086, 1483, 1882
MfeI CAATTG 1 cut(s) 1217
MhlI GDGCHC 2 cut(s) 123, 177
MlyI GAGTC 4 cut(s) 131, 210, 530, 1335
MmeI TCCRAC 4 cut(s) 816, 1029, 1114, 1413
Mph1103I ATGCAT 1 cut(s) 1907
MroI TCCGGA 1 cut(s) 156
MroXI GAANNNNTTC 2 cut(s) 1127, 1618
MseI TTAA 5 cut(s) 264, 681, 1002, 1541, 1601
MslI CAYNNNNRTG 2 cut(s) 276, 1952
MspA1I CMGCKG 1 cut(s) 2095
MspI CCGG 5 cut(s) 130, 157, 163, 169, 184
MspR9I CCNGG 8 cut(s) 80, 125, 130, 163, 170, 184, 185, 344
MssI GTTTAAAC 1 cut(s) 1003
MunI CAATTG 1 cut(s) 1217
MvaI CCWGG 3 cut(s) 80, 125, 344
MvnI CGCG 1 cut(s) 726
MwoI GCNNNNNNNGC 3 cut(s) 544, 985, 1315
NciI CCSGG 5 cut(s) 130, 163, 170, 184, 185
NdeII GATC 4 cut(s) 433, 1086, 1483, 1882
NlaIII CATG 3 cut(s) 505, 956, 1847
NlaIV GGNNCC 2 cut(s) 161, 167
NmuCI GTSAC 1 cut(s) 331
NsiI ATGCAT 1 cut(s) 1907
NspV TTCGAA 1 cut(s) 23
PciSI GCTCTTC 4 cut(s) 777, 978, 1053, 1161
PdmI GAANNNNTTC 2 cut(s) 1127, 1618
PfeI GAWTC 9 cut(s) 20, 631, 740, 941, 1112, 1208, 1325, 1409, 1693
PflMI CCANNNNNTGG 1 cut(s) 343
PfoI TCCNGGA 1 cut(s) 168
PkrI GCNGC 3 cut(s) 85, 978, 2097
PleI GAGTC 4 cut(s) 131, 209, 529, 1335
PmeI GTTTAAAC 1 cut(s) 1003
PpsI GAGTC 4 cut(s) 131, 209, 529, 1335
PsiI TTATAA 1 cut(s) 1478
Psp124BI GAGCTC 2 cut(s) 123, 177
Psp6I CCWGG 3 cut(s) 78, 123, 342
PspEI GGTNACC 1 cut(s) 331
PspFI CCCAGC 1 cut(s) 1768
PspGI CCWGG 3 cut(s) 78, 123, 342
PspN4I GGNNCC 2 cut(s) 161, 167
PspPI GGNCC 3 cut(s) 127, 159, 181
PvuII CAGCTG 1 cut(s) 2095
RsaI GTAC 2 cut(s) 1287, 1501
RsaNI GTAC 2 cut(s) 1286, 1500
RseI CAYNNNNRTG 2 cut(s) 276, 1952
SacI GAGCTC 2 cut(s) 123, 177
SapI GCTCTTC 4 cut(s) 777, 978, 1053, 1161
SaqAI TTAA 5 cut(s) 264, 681, 1002, 1541, 1601
SatI GCNGC 3 cut(s) 84, 977, 2096
Sau3AI GATC 4 cut(s) 433, 1086, 1483, 1882
Sau96I GGNCC 3 cut(s) 127, 159, 181
SchI GAGTC 4 cut(s) 131, 210, 530, 1335
ScrFI CCNGG 8 cut(s) 80, 125, 130, 163, 170, 184, 185, 344
SduI GDGCHC 2 cut(s) 123, 177
SfaNI GCATC 4 cut(s) 672, 889, 1576, 1789
SfuI TTCGAA 1 cut(s) 23
SinI GGWCC 1 cut(s) 159
SmaI CCCGGG 1 cut(s) 185
SmiMI CAYNNNNRTG 2 cut(s) 276, 1952
SmlI CTYRAG 5 cut(s) 176, 704, 1118, 1413, 1922
SmoI CTYRAG 5 cut(s) 176, 704, 1118, 1413, 1922
SsiI CCGC 3 cut(s) 52, 83, 141
SspI AATATT 1 cut(s) 1684
SstI GAGCTC 2 cut(s) 123, 177
StyD4I CCNGG 8 cut(s) 78, 123, 128, 161, 168, 182, 183, 342
StyI CCWWGG 1 cut(s) 894
TaaI ACNGT 3 cut(s) 16, 225, 1644
TaiI ACGT 2 cut(s) 276, 1270
TaqI TCGA 7 cut(s) 23, 63, 135, 1085, 1206, 1944, 2022
TaqII GACCGA 1 cut(s) 1887
TatI WGTACW 2 cut(s) 1285, 1499
TauI GCSGC 1 cut(s) 86
TfiI GAWTC 9 cut(s) 20, 631, 740, 941, 1112, 1208, 1325, 1409, 1693
Tru1I TTAA 5 cut(s) 264, 681, 1002, 1541, 1601
Tru9I TTAA 5 cut(s) 264, 681, 1002, 1541, 1601
TscAI CASTG 5 cut(s) 230, 1453, 1822, 1834, 2023
TseFI GTSAC 1 cut(s) 331
TseI GCWGC 2 cut(s) 976, 2095
Tsp45I GTSAC 1 cut(s) 331
TspDTI ATGAA 5 cut(s) 773, 779, 969, 980, 1863
TspMI CCCGGG 1 cut(s) 183
TspRI CASTG 5 cut(s) 230, 1453, 1822, 1834, 2023
Van91I CCANNNNNTGG 1 cut(s) 343
VpaK11BI GGWCC 1 cut(s) 159
XapI RAATTY 2 cut(s) 1537, 1787
XmaI CCCGGG 1 cut(s) 183
XmnI GAANNNNTTC 2 cut(s) 1127, 1618
Zsp2I ATGCAT 1 cut(s) 1907
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.