Rroxscaffold_7G00169760

Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0003402
Physical Location & Seq
Reverse (-)
10307570 .. 10309729
2160 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00169760.1

Sequence Viewer

Length: 1176 bp
ATGCAGCTCAATATTGTTGAAGGAAAGATGCAATATTTGTATGATGAGAATGGCAGGCGTTACCTTGATGCATTTGCTGGGATAGTGACGGTTTCTGCTGGACATTGCCATCCAGATGTTTTAAATGCGATCTTTGAGCAGAGCAAGCTTCTGCAGCATGCCACAACTATATATTTACACCATGCAATAGCTGATTTTGCAGAGGCATTGGCTTCAAAATTTTCCTGGAAATCTAAAGAAGCAAATGAGTTAGCAATGCTGATGGCTCGTCTATACACTGGTAGTCTGGAAATGATTTCTCTGAGAAATGGATATCACGGAGGAAGTTCTAATACAATGAGTTTGACGGCACTCAACACATGGAAGTATCCGATTCCACAGGGTGAAATTCATCATGCTGTGAATCCAGATCCATACCGTGGAGTCTTTGGCTCTGATGCAAAAAGTTATGCTAAAGATGTGCAAGATCACATTGATTTTGGAACTGCAGGAAAAGTGGCCGGATTTATATCTGAAACAATTCAGGGTGTTGGAGGAGCAGTTGAACTGGCTCCTGGATACCTAAAATTGGTATATGACATTGTTCGCAAGGCTGGTGGTGTCTGCATTGCTGATGAAGTTCAAACTGGATTTGGCCGCACAGGAACTCATTATTGGGGCTTTGAAACACAGGGGGTCATTCCTGATATAGTTACTATGGCCAAGGGCATTGGAAATGGATTGCCATTGGGAGCAGTGGTGACAACACCAGAAATAGCAAATGTACTAGCCCAGAAGACTCAATTCAACACTTATGGTGGAAACCCTGTATGCTCTGCAGGAGGACTTGCAGTGCTCAGAGTTATTGATAAAGAGAAACGTCAAGTTCATTCTGCAGATGTTGGCTCTGATTTGCTTGCTCGGTTGAGAGATCTTCAGCAAAGATATGAAATCATTGGAGATGTTCGAGGCCGGGGCTTAATGGTTGGGGTAGAACTTGTGACTGACAGGAATGAGAAGACACCTGCCAAGGCCGAAACTGCTATGTTATTTGAGAAACTCAGAGAGCTTGGAGTTCTAGTTGGGAAAGGGGGACTTCATGGAAATGTTTTCAGGATAAAACCACCACTGTGTTTCACCAAAGATGATGCAGATTTTCTTGTCGATGCTTTGGACTATTCAATGTCAAAATTGTGA

Protein Analysis

391

Amino Acids

42.47

Weight (kDa)

6.32

Isoelectric Point (pI)

19.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aminotran_3 PF00202 3 - 384 4.9e-80 Aminotransferase class-III
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1012
Acc36I ACCTGC 1 cut(s) 1012
AccB7I CCANNNNNTGG 1 cut(s) 419
AciI CCGC 1 cut(s) 637
AclWI GGATC 1 cut(s) 404
AcoI YGGCCR 3 cut(s) 498, 634, 699
AcsI RAATTY 2 cut(s) 218, 387
AcuI CTGAAG 1 cut(s) 899
AdeI CACNNNGTG 1 cut(s) 383
AfaI GTAC 1 cut(s) 765
AfiI CCNNNNNNNGG 2 cut(s) 419, 568
AgsI TTSAA 7 cut(s) 20, 216, 545, 623, 665, 787, 1161
AjnI CCWGG 2 cut(s) 224, 553
AjuI GAANNNNNNNTTGG 2 cut(s) 637, 669
AleI CACNNNNGTG 1 cut(s) 1108
AluBI AGCT 4 cut(s) 7, 148, 191, 1048
AluI AGCT 4 cut(s) 7, 148, 191, 1048
Alw21I GWGCWC 1 cut(s) 837
AlwI GGATC 1 cut(s) 404
AoxI GGCC 5 cut(s) 498, 634, 699, 949, 1011
ApeKI GCWGC 2 cut(s) 4, 154
ApoI RAATTY 2 cut(s) 218, 387
Asp700I GAANNNNTTC 1 cut(s) 519
AsuC2I CCSGG 1 cut(s) 953
AsuHPI GGTGA 3 cut(s) 395, 751, 1108
BalI TGGCCA 1 cut(s) 701
BarI GAAGNNNNNNTAC 2 cut(s) 316, 348
BbsI GAAGAC 2 cut(s) 782, 1004
Bbv12I GWGCWC 1 cut(s) 837
BbvI GCAGC 2 cut(s) 16, 166
BccI CCATC 2 cut(s) 117, 256
BceAI ACGGC 1 cut(s) 363
BciT130I CCWGG 2 cut(s) 226, 555
BciVI GTATCC 2 cut(s) 378, 551
BcnI CCSGG 1 cut(s) 953
BfaI CTAG 2 cut(s) 767, 1058
BfmI CTRYAG 4 cut(s) 152, 486, 816, 873
BfuAI ACCTGC 1 cut(s) 1012
BfuI GTATCC 2 cut(s) 378, 551
BglII AGATCT 1 cut(s) 910
BisI GCNGC 3 cut(s) 5, 155, 637
BlsI GCNGC 3 cut(s) 6, 156, 638
Bme1390I CCNGG 3 cut(s) 226, 555, 953
BmiI GGNNCC 1 cut(s) 552
BmrFI CCNGG 3 cut(s) 226, 555, 953
BmsI GCATC 5 cut(s) 18, 58, 427, 1117, 1135
BpiI GAAGAC 2 cut(s) 782, 1004
BpuMI CCSGG 1 cut(s) 953
BsaBI GATNNNNATC 1 cut(s) 508
BsaJI CCNNGG 4 cut(s) 418, 702, 952, 1008
BsaXI ACNNNNNCTCC 6 cut(s) 525, 555, 723, 753, 1044, 1074
Bsc4I CCNNNNNNNGG 2 cut(s) 419, 568
Bse1I ACTGG 3 cut(s) 283, 552, 631
Bse3DI GCAATG 3 cut(s) 103, 261, 606
Bse8I GATNNNNATC 1 cut(s) 508
BseBI CCWGG 2 cut(s) 226, 555
BseDI CCNNGG 4 cut(s) 418, 702, 952, 1008
BseGI GGATG 1 cut(s) 109
BseJI GATNNNNATC 1 cut(s) 508
BseLI CCNNNNNNNGG 2 cut(s) 419, 568
BseMI GCAATG 3 cut(s) 103, 261, 606
BseMII CTCAG 3 cut(s) 293, 850, 1054
BseNI ACTGG 3 cut(s) 283, 552, 631
BseRI GAGGAG 1 cut(s) 549
BseXI GCAGC 2 cut(s) 16, 166
BseYI CCCAGC 1 cut(s) 77
BshFI GGCC 5 cut(s) 500, 636, 701, 951, 1013
BsiHKAI GWGCWC 1 cut(s) 837
BsiSI CCGG 2 cut(s) 501, 952
BslFI GGGAC 1 cut(s) 1086
BslI CCNNNNNNNGG 2 cut(s) 419, 568
BsmFI GGGAC 1 cut(s) 1086
BsnI GGCC 5 cut(s) 500, 636, 701, 951, 1013
Bsp1286I GDGCHC 1 cut(s) 837
Bsp143I GATC 4 cut(s) 129, 409, 466, 910
BspACI CCGC 1 cut(s) 637
BspANI GGCC 5 cut(s) 500, 636, 701, 951, 1013
BspCNI CTCAG 3 cut(s) 294, 849, 1053
BspLI GGNNCC 1 cut(s) 552
BspMAI CTGCAG 4 cut(s) 156, 490, 820, 877
BspMI ACCTGC 1 cut(s) 1012
BspPI GGATC 1 cut(s) 404
BsrDI GCAATG 3 cut(s) 103, 261, 606
BsrI ACTGG 3 cut(s) 283, 552, 631
BssECI CCNNGG 4 cut(s) 418, 702, 952, 1008
BssMI GATC 4 cut(s) 129, 409, 466, 910
BssT1I CCWWGG 2 cut(s) 702, 1008
Bst2UI CCWGG 2 cut(s) 226, 555
Bst4CI ACNGT 3 cut(s) 91, 419, 1110
BstC8I GCNNGC 4 cut(s) 56, 146, 159, 897
BstDEI CTNAG 3 cut(s) 302, 836, 1040
BstDSI CCRYGG 1 cut(s) 418
BstF5I GGATG 1 cut(s) 109
BstKTI GATC 4 cut(s) 132, 412, 469, 913
BstMBI GATC 4 cut(s) 129, 409, 466, 910
BstMWI GCNNNNNNNGC 4 cut(s) 145, 154, 197, 1019
BstNI CCWGG 2 cut(s) 226, 555
BstNSI RCATGY 1 cut(s) 161
BstSCI CCNGG 3 cut(s) 224, 553, 951
BstSFI CTRYAG 4 cut(s) 152, 486, 816, 873
BstV1I GCAGC 2 cut(s) 16, 166
BstV2I GAAGAC 2 cut(s) 782, 1004
BstX2I RGATCY 2 cut(s) 409, 910
BstYI RGATCY 2 cut(s) 409, 910
BsuI GTATCC 2 cut(s) 378, 551
BsuRI GGCC 5 cut(s) 500, 636, 701, 951, 1013
BtgI CCRYGG 1 cut(s) 418
BtsCI GGATG 1 cut(s) 109
BtsI GCAGTG 2 cut(s) 741, 837
BtsIMutI CAGTG 4 cut(s) 276, 741, 837, 1106
BveI ACCTGC 1 cut(s) 1012
Cac8I GCNNGC 4 cut(s) 56, 146, 159, 897
Csp6I GTAC 1 cut(s) 764
CspCI CAANNNNNGTGG 2 cut(s) 577, 612
CviAII CATG 5 cut(s) 158, 182, 360, 395, 1079
CviQI GTAC 1 cut(s) 764
DdeI CTNAG 3 cut(s) 302, 836, 1040
DpnI GATC 4 cut(s) 131, 411, 468, 912
DpnII GATC 4 cut(s) 129, 409, 466, 910
DraI TTTAAA 1 cut(s) 123
DraIII CACNNNGTG 1 cut(s) 383
EaeI YGGCCR 3 cut(s) 498, 634, 699
Eco130I CCWWGG 2 cut(s) 702, 1008
Eco32I GATATC 1 cut(s) 314
Eco57I CTGAAG 1 cut(s) 899
EcoRII CCWGG 2 cut(s) 224, 553
EcoRV GATATC 1 cut(s) 314
EcoT14I CCWWGG 2 cut(s) 702, 1008
EcoT22I ATGCAT 1 cut(s) 73
ErhI CCWWGG 2 cut(s) 702, 1008
FaeI CATG 5 cut(s) 161, 185, 363, 398, 1082
FalI AAGNNNNNCTT 2 cut(s) 1059, 1091
FaqI GGGAC 1 cut(s) 1086
FatI CATG 5 cut(s) 157, 181, 359, 394, 1078
Fnu4HI GCNGC 3 cut(s) 5, 155, 637
FokI GGATG 1 cut(s) 96
Fsp4HI GCNGC 3 cut(s) 5, 155, 637
FspBI CTAG 2 cut(s) 767, 1058
GluI GCNGC 3 cut(s) 5, 155, 637
GsaI CCCAGC 1 cut(s) 81
HaeIII GGCC 5 cut(s) 500, 636, 701, 951, 1013
HapII CCGG 2 cut(s) 501, 952
Hin1II CATG 5 cut(s) 161, 185, 363, 398, 1082
HindIII AAGCTT 1 cut(s) 146
HinfI GANTC 4 cut(s) 373, 403, 423, 778
HpaII CCGG 2 cut(s) 501, 952
HphI GGTGA 3 cut(s) 395, 751, 1108
Hpy188I TCNGA 7 cut(s) 303, 372, 436, 514, 839, 889, 1043
Hpy188III TCNNGA 5 cut(s) 113, 287, 407, 683, 1093
HpyAV CCTTC 1 cut(s) 14
HpyCH4III ACNGT 3 cut(s) 91, 419, 1110
HpyCH4IV ACGT 1 cut(s) 859
HpyF10VI GCNNNNNNNGC 4 cut(s) 145, 154, 197, 1019
HpyF3I CTNAG 3 cut(s) 302, 836, 1040
HpySE526I ACGT 1 cut(s) 859
Hsp92II CATG 5 cut(s) 161, 185, 363, 398, 1082
Kzo9I GATC 4 cut(s) 129, 409, 466, 910
LmnI GCTCC 3 cut(s) 536, 556, 731
Lsp1109I GCAGC 2 cut(s) 16, 166
LweI GCATC 5 cut(s) 18, 58, 427, 1117, 1135
MaeI CTAG 2 cut(s) 767, 1058
MaeII ACGT 1 cut(s) 859
MaeIII GTNAC 5 cut(s) 59, 85, 691, 739, 979
MalI GATC 4 cut(s) 131, 411, 468, 912
MboI GATC 4 cut(s) 129, 409, 466, 910
MboII GAAGA 3 cut(s) 787, 905, 1009
MflI RGATCY 2 cut(s) 409, 910
MhlI GDGCHC 1 cut(s) 837
MlsI TGGCCA 1 cut(s) 701
MluCI AATT 6 cut(s) 218, 387, 519, 566, 782, 1169
MluNI TGGCCA 1 cut(s) 701
MlyI GAGTC 2 cut(s) 432, 772
MmeI TCCRAC 1 cut(s) 511
MnlI CCTC 5 cut(s) 196, 314, 527, 815, 941
Mox20I TGGCCA 1 cut(s) 701
Mph1103I ATGCAT 1 cut(s) 73
MroXI GAANNNNTTC 1 cut(s) 519
MscI TGGCCA 1 cut(s) 701
MseI TTAA 2 cut(s) 122, 959
MslI CAYNNNNRTG 3 cut(s) 114, 1083, 1108
Msp20I TGGCCA 1 cut(s) 701
MspI CCGG 2 cut(s) 501, 952
MspR9I CCNGG 3 cut(s) 226, 555, 953
MvaI CCWGG 2 cut(s) 226, 555
MwoI GCNNNNNNNGC 4 cut(s) 145, 154, 197, 1019
NciI CCSGG 1 cut(s) 953
NdeII GATC 4 cut(s) 129, 409, 466, 910
NlaIII CATG 5 cut(s) 161, 185, 363, 398, 1082
NlaIV GGNNCC 1 cut(s) 552
NmuCI GTSAC 3 cut(s) 85, 739, 979
NsiI ATGCAT 1 cut(s) 73
NspI RCATGY 1 cut(s) 161
OliI CACNNNNGTG 1 cut(s) 1108
PaeI GCATGC 1 cut(s) 161
PaqCI CACCTGC 1 cut(s) 1012
PdmI GAANNNNTTC 1 cut(s) 519
PfeI GAWTC 2 cut(s) 373, 403
PflMI CCANNNNNTGG 1 cut(s) 419
PfoI TCCNGGA 2 cut(s) 224, 553
PkrI GCNGC 3 cut(s) 6, 156, 638
PleI GAGTC 2 cut(s) 431, 772
PpsI GAGTC 2 cut(s) 431, 772
Psp6I CCWGG 2 cut(s) 224, 553
PspFI CCCAGC 1 cut(s) 77
PspGI CCWGG 2 cut(s) 224, 553
PspN4I GGNNCC 1 cut(s) 552
PstI CTGCAG 4 cut(s) 156, 490, 820, 877
PsuI RGATCY 2 cut(s) 409, 910
RsaI GTAC 1 cut(s) 765
RsaNI GTAC 1 cut(s) 764
RseI CAYNNNNRTG 3 cut(s) 114, 1083, 1108
SaqAI TTAA 2 cut(s) 122, 959
SatI GCNGC 3 cut(s) 5, 155, 637
Sau3AI GATC 4 cut(s) 129, 409, 466, 910
SchI GAGTC 2 cut(s) 432, 772
ScrFI CCNGG 3 cut(s) 226, 555, 953
SduI GDGCHC 1 cut(s) 837
SetI ASST 8 cut(s) 9, 66, 150, 193, 564, 862, 1006, 1050
SfaNI GCATC 5 cut(s) 18, 58, 427, 1117, 1135
SfcI CTRYAG 4 cut(s) 152, 486, 816, 873
SmiMI CAYNNNNRTG 3 cut(s) 114, 1083, 1108
SphI GCATGC 1 cut(s) 161
Sse9I AATT 6 cut(s) 218, 387, 519, 566, 782, 1169
SsiI CCGC 1 cut(s) 637
SspI AATATT 2 cut(s) 13, 35
SspMI CTAG 2 cut(s) 767, 1058
StyD4I CCNGG 3 cut(s) 224, 553, 951
StyI CCWWGG 2 cut(s) 702, 1008
TaaI ACNGT 3 cut(s) 91, 419, 1110
TaiI ACGT 1 cut(s) 862
TaqI TCGA 2 cut(s) 946, 1143
TasI AATT 6 cut(s) 218, 387, 519, 566, 782, 1169
TatI WGTACW 1 cut(s) 763
TauI GCSGC 1 cut(s) 639
TfiI GAWTC 2 cut(s) 373, 403
Tru1I TTAA 2 cut(s) 122, 959
Tru9I TTAA 2 cut(s) 122, 959
TscAI CASTG 4 cut(s) 283, 741, 837, 1113
TseFI GTSAC 3 cut(s) 85, 739, 979
TseI GCWGC 2 cut(s) 4, 154
Tsp45I GTSAC 3 cut(s) 85, 739, 979
TspDTI ATGAA 5 cut(s) 380, 630, 857, 942, 1067
TspGWI ACGGA 1 cut(s) 333
TspRI CASTG 4 cut(s) 283, 741, 837, 1113
Van91I CCANNNNNTGG 1 cut(s) 419
XapI RAATTY 2 cut(s) 218, 387
XceI RCATGY 1 cut(s) 161
XmnI GAANNNNTTC 1 cut(s) 519
XspI CTAG 2 cut(s) 767, 1058
Zsp2I ATGCAT 1 cut(s) 73
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.