Rw2G020240

Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0003402
Physical Location & Seq
Reverse (-)
27467807 .. 27473690
5884 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G020240.1

Sequence Viewer

Length: 1425 bp
ATGGCGTTTCACAGGCACCTTTTCAGAAGCGTTATGAAACCCAAGATTGGTGGTGCTCGGGTGTCTTTCTCTACCGTTCAATCTGCCACTGCTGACGAAGCGGCTGTGCCTTCTTTGCCGCCGTTCGATTACGAGCCGAGGCCCTACAATGGGCCACGCGCCGATCAGGTCTTCGAGAAGCGGAAGAAGTTTCTGGGTCCTTCTCTTTTCCACTTCTACCAGAAGCCTATCAATATTGTAGAAGGGAAGATGCAATATCTGTTTGATGAGGATGGCAGGCGCTACCTCGATGCTTTTGCTGGAATCGTGACTGTTTCTTGCGGACATTGTCATCCGGTTGTTTTGAATGCTATCATGGAGCAGAGCAGGCTTCTTCAGCACGCTACGACCATATACTTGCACCACACGATCGCAGATTTCGCAGAGGCATTGGCTTCAAAAATGCCTGGAAATTTGAAGGTGGTATACTTTGTAAATTCTGGGACAGAAGCAAATGAGTTGGCGATGCTGATGGCTCGTCTGTACAGTGGTAGTCTTGGTATGATTGCGTTGAGAAATGCATATCATGGTGGAAGTTCCAATACGATTGGTTTGACAGCGCTGAACACATGGAAGTATCCAATTCCACAGGGTGAAATTCATCACGTTGTAAATCCAGATCCATACCGTGGAGTCTTTGGCTCTGATGCCAAAAGTTATGCTGAAGATGTGCAAGATCACATTGATTATGGGACTTCTGGAAAAGTTGCTGGATTCATATCGGAAACAATTCAGGGAGTTGGAGGAGCAGTTGAATTGGCCCCTGGTTATTTGAAAATGGTTTATGACATTGTTCGAAAGGCTGGTGGTGTCTGCATCGCTGATGAAGTACAAACTGGGTTTGGCCGCACTGGAAGTCACTATTGGGGCTTTGAAACTCAGGATGTCATTCCTGATATTGTAACTATGGCAAAGGGCATTGGAAATGGATTGCCCTTGGGAGCAGTGGTGACTACACCAGAAATCGCAAGTGTACTAGCACAAAAGACACATTTCAACACATATGGTGGAAACCCTGTATGCTCTGCGGGAGGGCTTGCAGTTCTTAAAGTTATTGATGAGGAGAAGCGTCAAACTCACTCCGCTGATGTTGGCTCTCACTTGCTTGGTCGTTTGAGAGATCTTCAGCAGAGACACGACATCATTGGTGATGTGAGAGGCCGAGGATTGATGGTCGGAGTAGAGCTCGTGACCGACAGGACTGAAAAGACACCTGCTAAGGCAGAAACTGCGGTGTTGTTTGAAAACCTTAGAGAGCTAGGAATTCTGGTTGGGAAAGGAGGTTTACACGGCAACGTATTTAGGGTAAAACCACCAATGTGTTTTACCAAGGACGATGCAGATTTTCTTGTAGATGCAATGGATTATTCCATGTCAAAGTTGTGA

Protein Analysis

474

Amino Acids

51.65

Weight (kDa)

6.65

Isoelectric Point (pI)

29.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aminotran_3 PF00202 76 - 467 2.8e-86 Aminotransferase class-III
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1261
Acc36I ACCTGC 1 cut(s) 1261
AccB1I GGYRCC 1 cut(s) 15
AccB7I CCANNNNNTGG 1 cut(s) 668
AccI GTMKAC 1 cut(s) 465
AccII CGCG 1 cut(s) 159
AciI CCGC 8 cut(s) 101, 119, 181, 321, 886, 1067, 1122, 1271
AclWI GGATC 1 cut(s) 653
AcoI YGGCCR 1 cut(s) 883
AcsI RAATTY 4 cut(s) 451, 475, 636, 1302
AcuI CTGAAG 3 cut(s) 359, 723, 1148
AdeI CACNNNGTG 1 cut(s) 632
AfaI GTAC 3 cut(s) 524, 870, 1014
AfeI AGCGCT 1 cut(s) 600
AfiI CCNNNNNNNGG 4 cut(s) 47, 149, 150, 668
AgsI TTSAA 9 cut(s) 80, 346, 438, 457, 794, 814, 914, 1036, 1283
AjnI CCWGG 2 cut(s) 445, 802
AjuI GAANNNNNNNTTGG 2 cut(s) 886, 918
AleI CACNNNNGTG 1 cut(s) 1357
AluBI AGCT 2 cut(s) 1225, 1297
AluI AGCT 2 cut(s) 1225, 1297
Alw21I GWGCWC 2 cut(s) 58, 1227
Alw26I GTCTC 1 cut(s) 1165
AlwI GGATC 1 cut(s) 653
AlwNI CAGNNNCTG 1 cut(s) 1268
Ama87I CYCGRG 1 cut(s) 57
Aor51HI AGCGCT 1 cut(s) 600
AoxI GGCC 5 cut(s) 140, 152, 798, 883, 1198
ApoI RAATTY 4 cut(s) 451, 475, 636, 1302
Asp700I GAANNNNTTC 1 cut(s) 768
AspLEI GCGC 3 cut(s) 161, 282, 601
AspS9I GGNCC 4 cut(s) 141, 152, 197, 799
AsuHPI GGTGA 3 cut(s) 644, 1000, 1199
AsuII TTCGAA 1 cut(s) 835
AvaI CYCGRG 1 cut(s) 57
AvaII GGWCC 1 cut(s) 197
BanI GGYRCC 1 cut(s) 15
BanII GRGCYC 1 cut(s) 1227
BarI GAAGNNNNNNTAC 4 cut(s) 449, 481, 565, 597
BauI CACGAG 1 cut(s) 1226
BbsI GAAGAC 1 cut(s) 163
Bbv12I GWGCWC 2 cut(s) 58, 1227
BccI CCATC 3 cut(s) 266, 505, 1204
BceAI ACGGC 2 cut(s) 106, 1345
BcgI CGANNNNNNTGC 2 cut(s) 278, 312
BciT130I CCWGG 2 cut(s) 447, 804
BciVI GTATCC 1 cut(s) 627
BcoDI GTCTC 1 cut(s) 1165
BfaI CTAG 2 cut(s) 1016, 1298
BfoI RGCGCY 2 cut(s) 283, 602
BfuAI ACCTGC 1 cut(s) 1261
BfuI GTATCC 1 cut(s) 627
BglII AGATCT 1 cut(s) 1159
BisI GCNGC 3 cut(s) 102, 119, 886
BlsI GCNGC 3 cut(s) 103, 120, 887
Bme1390I CCNGG 2 cut(s) 447, 804
Bme18I GGWCC 1 cut(s) 197
BmeT110I CYCGRG 1 cut(s) 57
BmgT120I GGNCC 4 cut(s) 141, 152, 197, 799
BmiI GGNNCC 3 cut(s) 17, 198, 801
BmrFI CCNGG 2 cut(s) 447, 804
BmrI ACTGGG 1 cut(s) 885
BmsI GCATC 7 cut(s) 240, 280, 495, 676, 864, 1366, 1384
BmuI ACTGGG 1 cut(s) 885
BpiI GAAGAC 1 cut(s) 163
BplI GAGNNNNNCTC 2 cut(s) 1209, 1241
Bpu10I CCTNAGC 1 cut(s) 1257
Bpu14I TTCGAA 1 cut(s) 835
BsaBI GATNNNNATC 1 cut(s) 757
BsaJI CCNNGG 6 cut(s) 137, 667, 802, 975, 1201, 1368
BsaWI WCCGGW 1 cut(s) 334
BsaXI ACNNNNNCTCC 4 cut(s) 774, 804, 972, 1002
Bsc4I CCNNNNNNNGG 4 cut(s) 47, 149, 150, 668
Bse1I ACTGG 2 cut(s) 880, 895
Bse3DI GCAATG 1 cut(s) 1404
Bse8I GATNNNNATC 1 cut(s) 757
BseBI CCWGG 2 cut(s) 447, 804
BseDI CCNNGG 6 cut(s) 137, 667, 802, 975, 1201, 1368
BseGI GGATG 3 cut(s) 277, 331, 928
BseJI GATNNNNATC 1 cut(s) 757
BseLI CCNNNNNNNGG 4 cut(s) 47, 149, 150, 668
BseMI GCAATG 1 cut(s) 1404
BseMII CTCAG 1 cut(s) 932
BseNI ACTGG 2 cut(s) 880, 895
BseRI GAGGAG 2 cut(s) 798, 1115
Bsh1236I CGCG 1 cut(s) 159
Bsh1285I CGRYCG 1 cut(s) 411
BshFI GGCC 5 cut(s) 142, 154, 800, 885, 1200
BshNI GGYRCC 1 cut(s) 15
BsiEI CGRYCG 1 cut(s) 411
BsiHKAI GWGCWC 2 cut(s) 58, 1227
BsiHKCI CYCGRG 1 cut(s) 57
BsiSI CCGG 1 cut(s) 335
BslFI GGGAC 2 cut(s) 496, 745
BslI CCNNNNNNNGG 4 cut(s) 47, 149, 150, 668
BsmAI GTCTC 1 cut(s) 1165
BsmFI GGGAC 2 cut(s) 496, 745
BsmI GAATGC 1 cut(s) 352
BsnI GGCC 5 cut(s) 142, 154, 800, 885, 1200
BsoBI CYCGRG 1 cut(s) 57
Bsp119I TTCGAA 1 cut(s) 835
Bsp1286I GDGCHC 2 cut(s) 58, 1227
Bsp1407I TGTACA 1 cut(s) 522
Bsp143I GATC 5 cut(s) 163, 408, 658, 715, 1159
BspACI CCGC 8 cut(s) 101, 119, 181, 321, 886, 1067, 1122, 1271
BspANI GGCC 5 cut(s) 142, 154, 800, 885, 1200
BspCNI CTCAG 1 cut(s) 931
BspFNI CGCG 1 cut(s) 159
BspLI GGNNCC 3 cut(s) 17, 198, 801
BspMI ACCTGC 1 cut(s) 1261
BspPI GGATC 1 cut(s) 653
BspT104I TTCGAA 1 cut(s) 835
BspT107I GGYRCC 1 cut(s) 15
BsrDI GCAATG 1 cut(s) 1404
BsrGI TGTACA 1 cut(s) 522
BsrI ACTGG 2 cut(s) 880, 895
BssECI CCNNGG 6 cut(s) 137, 667, 802, 975, 1201, 1368
BssMI GATC 5 cut(s) 163, 408, 658, 715, 1159
BssNAI GTATAC 1 cut(s) 466
BssSI CACGAG 1 cut(s) 1226
BssT1I CCWWGG 2 cut(s) 975, 1368
Bst1107I GTATAC 1 cut(s) 466
Bst2BI CACGAG 1 cut(s) 1226
Bst2UI CCWGG 2 cut(s) 447, 804
Bst4CI ACNGT 4 cut(s) 76, 313, 527, 668
BstAPI GCANNNNNTGC 1 cut(s) 1268
BstAUI TGTACA 1 cut(s) 522
BstBI TTCGAA 1 cut(s) 835
BstC8I GCNNGC 4 cut(s) 278, 368, 381, 1077
BstDEI CTNAG 3 cut(s) 918, 1257, 1289
BstDSI CCRYGG 1 cut(s) 667
BstF5I GGATG 3 cut(s) 277, 331, 928
BstFNI CGCG 1 cut(s) 159
BstH2I RGCGCY 2 cut(s) 283, 602
BstHHI GCGC 3 cut(s) 161, 282, 601
BstKTI GATC 5 cut(s) 166, 411, 661, 718, 1162
BstMAI GTCTC 1 cut(s) 1165
BstMBI GATC 5 cut(s) 163, 408, 658, 715, 1159
BstMCI CGRYCG 1 cut(s) 411
BstMWI GCNNNNNNNGC 6 cut(s) 98, 115, 367, 376, 419, 1268
BstNI CCWGG 2 cut(s) 447, 804
BstSCI CCNGG 2 cut(s) 445, 802
BstUI CGCG 1 cut(s) 159
BstV2I GAAGAC 1 cut(s) 163
BstX2I RGATCY 2 cut(s) 658, 1159
BstYI RGATCY 2 cut(s) 658, 1159
BstZ17I GTATAC 1 cut(s) 466
BsuI GTATCC 1 cut(s) 627
BsuRI GGCC 5 cut(s) 142, 154, 800, 885, 1200
BtgI CCRYGG 1 cut(s) 667
BtgZI GCGATG 2 cut(s) 518, 841
BtsCI GGATG 3 cut(s) 277, 331, 928
BtsI GCAGTG 2 cut(s) 87, 990
BtsIMutI CAGTG 4 cut(s) 87, 532, 888, 990
BveI ACCTGC 1 cut(s) 1261
Cac8I GCNNGC 4 cut(s) 278, 368, 381, 1077
CaiI CAGNNNCTG 1 cut(s) 1268
CfoI GCGC 3 cut(s) 161, 282, 601
Cfr13I GGNCC 4 cut(s) 141, 152, 197, 799
CseI GACGC 1 cut(s) 1097
Csp6I GTAC 3 cut(s) 523, 869, 1013
CspCI CAANNNNNGTGG 2 cut(s) 31, 66
CviAII CATG 4 cut(s) 355, 566, 609, 1411
CviQI GTAC 3 cut(s) 523, 869, 1013
DdeI CTNAG 3 cut(s) 918, 1257, 1289
DpnI GATC 5 cut(s) 165, 410, 660, 717, 1161
DpnII GATC 5 cut(s) 163, 408, 658, 715, 1159
DraIII CACNNNGTG 1 cut(s) 632
EaeI YGGCCR 1 cut(s) 883
Ecl136II GAGCTC 1 cut(s) 1225
Eco130I CCWWGG 2 cut(s) 975, 1368
Eco24I GRGCYC 1 cut(s) 1227
Eco47I GGWCC 1 cut(s) 197
Eco47III AGCGCT 1 cut(s) 600
Eco53kI GAGCTC 1 cut(s) 1225
Eco57I CTGAAG 3 cut(s) 359, 723, 1148
Eco88I CYCGRG 1 cut(s) 57
EcoICRI GAGCTC 1 cut(s) 1225
EcoO109I RGGNCCY 2 cut(s) 141, 197
EcoRI GAATTC 1 cut(s) 1302
EcoRII CCWGG 2 cut(s) 445, 802
EcoT14I CCWWGG 2 cut(s) 975, 1368
EcoT22I ATGCAT 1 cut(s) 562
EcoT38I GRGCYC 1 cut(s) 1227
ErhI CCWWGG 2 cut(s) 975, 1368
FaeI CATG 4 cut(s) 358, 569, 612, 1414
FaqI GGGAC 2 cut(s) 496, 745
FatI CATG 4 cut(s) 354, 565, 608, 1410
FauI CCCGC 1 cut(s) 1060
FauNDI CATATG 1 cut(s) 1042
FblI GTMKAC 1 cut(s) 465
Fnu4HI GCNGC 3 cut(s) 102, 119, 886
FokI GGATG 3 cut(s) 284, 318, 935
FriOI GRGCYC 1 cut(s) 1227
Fsp4HI GCNGC 3 cut(s) 102, 119, 886
FspBI CTAG 2 cut(s) 1016, 1298
GlaI GCGC 3 cut(s) 160, 281, 600
GluI GCNGC 3 cut(s) 102, 119, 886
HaeII RGCGCY 2 cut(s) 283, 602
HaeIII GGCC 5 cut(s) 142, 154, 800, 885, 1200
HapII CCGG 1 cut(s) 335
HgaI GACGC 1 cut(s) 1097
HhaI GCGC 3 cut(s) 161, 282, 601
Hin1II CATG 4 cut(s) 358, 569, 612, 1414
Hin6I GCGC 3 cut(s) 159, 280, 599
HinP1I GCGC 3 cut(s) 159, 280, 599
HinfI GANTC 3 cut(s) 303, 672, 753
HpaII CCGG 1 cut(s) 335
HphI GGTGA 3 cut(s) 644, 1000, 1199
Hpy166II GTNNAC 3 cut(s) 466, 1013, 1325
Hpy188I TCNGA 4 cut(s) 26, 685, 763, 1217
Hpy188III TCNNGA 7 cut(s) 175, 307, 656, 738, 920, 932, 1228
Hpy8I GTNNAC 3 cut(s) 466, 1013, 1325
HpyAV CCTTC 4 cut(s) 120, 210, 236, 451
HpyCH4III ACNGT 4 cut(s) 76, 313, 527, 668
HpyCH4IV ACGT 2 cut(s) 645, 1335
HpyCH4V TGCA 8 cut(s) 253, 400, 560, 712, 855, 1079, 1379, 1397
HpyF10VI GCNNNNNNNGC 6 cut(s) 98, 115, 367, 376, 419, 1268
HpyF3I CTNAG 3 cut(s) 918, 1257, 1289
HpySE526I ACGT 2 cut(s) 645, 1335
Hsp92II CATG 4 cut(s) 358, 569, 612, 1414
HspAI GCGC 3 cut(s) 159, 280, 599
Kzo9I GATC 5 cut(s) 163, 408, 658, 715, 1159
LmnI GCTCC 3 cut(s) 358, 785, 980
LweI GCATC 7 cut(s) 240, 280, 495, 676, 864, 1366, 1384
MaeI CTAG 2 cut(s) 1016, 1298
MaeII ACGT 2 cut(s) 645, 1335
MaeIII GTNAC 5 cut(s) 307, 896, 940, 988, 1228
MalI GATC 5 cut(s) 165, 410, 660, 717, 1161
MboI GATC 5 cut(s) 163, 408, 658, 715, 1159
MboII GAAGA 6 cut(s) 163, 196, 259, 365, 716, 1154
MflI RGATCY 2 cut(s) 658, 1159
MhlI GDGCHC 2 cut(s) 58, 1227
MluCI AATT 7 cut(s) 451, 475, 621, 636, 768, 794, 1302
MlyI GAGTC 1 cut(s) 681
MmeI TCCRAC 2 cut(s) 760, 1195
Mph1103I ATGCAT 1 cut(s) 562
MroXI GAANNNNTTC 1 cut(s) 768
MseI TTAA 1 cut(s) 1086
MslI CAYNNNNRTG 1 cut(s) 1357
MspA1I CMGCKG 1 cut(s) 1124
MspI CCGG 1 cut(s) 335
MspR9I CCNGG 2 cut(s) 447, 804
Mva1269I GAATGC 1 cut(s) 352
MvaI CCWGG 2 cut(s) 447, 804
MvnI CGCG 1 cut(s) 159
MwoI GCNNNNNNNGC 6 cut(s) 98, 115, 367, 376, 419, 1268
NdeI CATATG 1 cut(s) 1042
NdeII GATC 5 cut(s) 163, 408, 658, 715, 1159
NlaIII CATG 4 cut(s) 358, 569, 612, 1414
NlaIV GGNNCC 3 cut(s) 17, 198, 801
NmeAIII GCCGAG 2 cut(s) 162, 1226
NmuCI GTSAC 4 cut(s) 307, 896, 988, 1228
NsiI ATGCAT 1 cut(s) 562
NspV TTCGAA 1 cut(s) 835
OliI CACNNNNGTG 1 cut(s) 1357
PaqCI CACCTGC 1 cut(s) 1261
PctI GAATGC 1 cut(s) 352
PdmI GAANNNNTTC 1 cut(s) 768
PfeI GAWTC 2 cut(s) 303, 753
PflFI GACNNNGTC 1 cut(s) 327
PflMI CCANNNNNTGG 1 cut(s) 668
PkrI GCNGC 3 cut(s) 103, 120, 887
Ple19I CGATCG 1 cut(s) 411
PleI GAGTC 1 cut(s) 680
PpsI GAGTC 1 cut(s) 680
PpuMI RGGWCCY 1 cut(s) 197
Psp124BI GAGCTC 1 cut(s) 1227
Psp5II RGGWCCY 1 cut(s) 197
Psp6I CCWGG 2 cut(s) 445, 802
PspGI CCWGG 2 cut(s) 445, 802
PspN4I GGNNCC 3 cut(s) 17, 198, 801
PspPI GGNCC 4 cut(s) 141, 152, 197, 799
PspPPI RGGWCCY 1 cut(s) 197
PstNI CAGNNNCTG 1 cut(s) 1268
PsuI RGATCY 2 cut(s) 658, 1159
PsyI GACNNNGTC 1 cut(s) 327
PvuI CGATCG 1 cut(s) 411
RsaI GTAC 3 cut(s) 524, 870, 1014
RsaNI GTAC 3 cut(s) 523, 869, 1013
RseI CAYNNNNRTG 1 cut(s) 1357
SacI GAGCTC 1 cut(s) 1227
SaqAI TTAA 1 cut(s) 1086
SatI GCNGC 3 cut(s) 102, 119, 886
Sau3AI GATC 5 cut(s) 163, 408, 658, 715, 1159
Sau96I GGNCC 4 cut(s) 141, 152, 197, 799
SchI GAGTC 1 cut(s) 681
ScrFI CCNGG 2 cut(s) 447, 804
SduI GDGCHC 2 cut(s) 58, 1227
SfaNI GCATC 7 cut(s) 240, 280, 495, 676, 864, 1366, 1384
SfuI TTCGAA 1 cut(s) 835
SinI GGWCC 1 cut(s) 197
SmiMI CAYNNNNRTG 1 cut(s) 1357
Sse9I AATT 7 cut(s) 451, 475, 621, 636, 768, 794, 1302
SsiI CCGC 8 cut(s) 101, 119, 181, 321, 886, 1067, 1122, 1271
SspI AATATT 1 cut(s) 235
SspMI CTAG 2 cut(s) 1016, 1298
SstI GAGCTC 1 cut(s) 1227
StyD4I CCNGG 2 cut(s) 445, 802
StyI CCWWGG 2 cut(s) 975, 1368
TaaI ACNGT 4 cut(s) 76, 313, 527, 668
TaiI ACGT 2 cut(s) 648, 1338
TaqI TCGA 4 cut(s) 126, 174, 288, 835
TaqII GACCGA 1 cut(s) 1247
TasI AATT 7 cut(s) 451, 475, 621, 636, 768, 794, 1302
TatI WGTACW 3 cut(s) 522, 868, 1012
TauI GCSGC 3 cut(s) 104, 121, 888
TfiI GAWTC 2 cut(s) 303, 753
Tru1I TTAA 1 cut(s) 1086
Tru9I TTAA 1 cut(s) 1086
TscAI CASTG 4 cut(s) 94, 532, 895, 990
TseFI GTSAC 4 cut(s) 307, 896, 988, 1228
Tsp45I GTSAC 4 cut(s) 307, 896, 988, 1228
TspDTI ATGAA 4 cut(s) 50, 629, 745, 879
TspRI CASTG 4 cut(s) 94, 532, 895, 990
Tth111I GACNNNGTC 1 cut(s) 327
Van91I CCANNNNNTGG 1 cut(s) 668
VpaK11BI GGWCC 1 cut(s) 197
XapI RAATTY 4 cut(s) 451, 475, 636, 1302
XmiI GTMKAC 1 cut(s) 465
XmnI GAANNNNTTC 1 cut(s) 768
XspI CTAG 2 cut(s) 1016, 1298
Zsp2I ATGCAT 1 cut(s) 562
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.