Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000007

Orthogroup ID

OG0004844

Physical Location & Seq

Genomic Coordinates Forward (+)

19049021 .. 19050928

Gene Structure 1908 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_7G00179500.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1908 bp

ATGTCATGTTCAAAAGGCAAGCTTTCATCTTTATTCACGAACCCAGAAAGACCCACTTGTTCAAATTCAACCAAGCTTTCATCTTTTTTCTTCACAAACCAAGTTACAGCTAAACCCACTTCTTCATATCCCACAACTTCAACAGAAACCCCAACTCCCAGTGTTCCAATCCAAACCCCTCTTGAAAATTTCCTCCAAACAAACTGCAAGTCAGGTAATTTCACTCTCAATGAAGCACTTCACTATTTTGACCGCATGATTCTTATGCAACCTGTTCCTCCAATGGCCTCGTTCAATCGTTTATTAGGTGGACTTGCCAAGACTAAGCATTACTCTCATGTTTTCTCATTTTGCATCAAGTTAACATCTTCTGGGTTATTGCTTGATTTCATTACGCTTAATATTTTGCTGAATTGCTTTTGTAACCTGAAACGGGTTTGTGATGCTTTGGTGGCTCTGGGAAGTATGATTAGGAGGGGTTATAAACCGAGTGCTGTTACTTTTACTTCTTTGGTTAAAGGGTTTTGTATGGAGGATAGGATTGATGAGGCAGTGAGGTTGTTGGAGAAAATGATCAAGTTTCAGTGTCAGCCTACTGTGATGACTTGTGGTACTTTGATTAATGGGCTGTGCAGGGCAGGAAAGACTAGCGTGGCACTTAGGTTGCATGAAGATATGGCTAAGGGGAATGGTGGATTTGGTGTTTATTGTAAGCCGAATGTGGTTACTTATGGGATTATTATTGATAGGCTTTGTAAGGATGGCATGATAGATGAGGCAAAAGACCTTTTCTTAGAAATGAAAGATAGAGGGATTCTTCCTGATGTGGTTGTGTATAGTGCTCTGATACATGGTTTGTACTATTATGAAAAGTGGGAAGCTGGTAAAGCTCTGTTACATGAGATGGTGCATTGCGGTATCAAGCCTAATCTGGTGACGTTCAATGTGCTAGTAGCGGCACTTTGCAGAAGGGGAAATGTGAAAGAGAGCAGTGATTTGCTAAAGCTTATGGTACACAGAGGCATTAGTCCTGATTTATTTACATACAACACTTTGATAGATGGCTTCTGTTTAGCAGGTTGCCTTAATGAAGCGAGAGAGATGTTTCATACTATTTCAAGTAGAGGGTGTCAACCTGATTCAGTTAGCTACAATGTGTTGATCAATGGATACTGCAAGAATTGTAAGATACATGAAGCTGTCAATGTTTACGAGGAAATGATCCGCAGAGGAGTTAGGCCAACTGTAGTAACGTATAATGCCTTATTAACTGGTCTGTTTAAGTTGGGAACAGTTCAGGATGCACAGAAGCTGTTTGCTGAGATGCAAACTCAGAACGTGCTACCTAATTCATCTACATATAAAATATTGTTGGACAGGCTTTGCAAAAATGATTGCATACCAGAGGCAATGGAAATATTTCATACTTTAGAAAATAGCAGCAGCTGTGAACTTAGTGTAGAAATTTTTAATTCACTTATTAATGGGTGTTGTAAGGCACGGAAGCTCGAAATTGCTTGGGACCTATATCACAAAATGTCAAAAAAAGGCCTGGTACCGGATGTTATAACATATAGCATGATGATTCATGGGCTCTGTATAGAAGGGCAACTCGAAAAGGCAAATGGTTTGTTTTTGGAAATGGAAGGAAACGGTTGTGCTCCAAACATCGTCACTTATAATACCCTTATGCGTGGTTTCTGCCAAAATAATCACTTGCAAAAGGTTGTTGAACTTCTTCATAAGATGTTGGAAAGAAATCTGTCACCAGATAACTCTACAATCTCCATTGTCATAGACTTGCTCTCGAAGGATGAAAGCTATCGGAAATGTTTGGACTGGCTTCCAACATTTCCTGCCAGTAGCCAATTGAAAGCTGAGGTGACAAATTTCAAGTCACTTGCATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000959 GO:0000963 GO:0000966 GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004016 GO:0004518 GO:0004519 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0006139 GO:0006396 GO:0006397 GO:0006725 GO:0006807 GO:0007275 GO:0008150 GO:0008152 GO:0009451 GO:0009791 GO:0009845 GO:0009975 GO:0009987 GO:0010467 GO:0016070 GO:0016071 GO:0016556 GO:0016787 GO:0016788 GO:0016829 GO:0016849 GO:0032501 GO:0032502 GO:0034641 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044424 GO:0044444 GO:0044464 GO:0046483 GO:0048856 GO:0071704 GO:0080156 GO:0090304 GO:0090305 GO:0090351 GO:0090615 GO:0097159 GO:0140053 GO:1900864 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PPR PPR_1 PPR_2 PPR_3

Protein Analysis

635

Amino Acids

70.99

Weight (kDa)

8.38

Isoelectric Point (pI)

27.69

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PPR_2	PF13041	131 - 176	1.2e-09	PPR repeat family
PPR_1	PF12854	159 - 191	6.9e-11	PPR repeat
PPR_2	PF13041	163 - 212	1.1e-12	PPR repeat family
PPR	PF01535	166 - 196	5.1e-06	PPR repeat
PPR_1	PF12854	195 - 226	2.5e-07	PPR repeat
PPR_1	PF12854	237 - 268	9.5e-12	PPR repeat
PPR_2	PF13041	239 - 286	9.3e-16	PPR repeat family
PPR	PF01535	242 - 272	3.1e-08	PPR repeat
PPR_3	PF13812	263 - 320	1.8e-09	Pentatricopeptide repeat domain
PPR_2	PF13041	274 - 323	2e-11	PPR repeat family
PPR_1	PF12854	306 - 338	2.3e-08	PPR repeat
PPR_2	PF13041	310 - 357	1.2e-14	PPR repeat family
PPR_3	PF13812	333 - 393	1.6e-09	Pentatricopeptide repeat domain
PPR_1	PF12854	340 - 370	1e-11	PPR repeat
PPR_2	PF13041	347 - 393	1.5e-15	PPR repeat family
PPR	PF01535	347 - 377	1e-06	PPR repeat
PPR_1	PF12854	375 - 407	9.1e-12	PPR repeat
PPR_2	PF13041	379 - 428	2e-19	PPR repeat family
PPR	PF01535	382 - 412	6.7e-10	PPR repeat
PPR_3	PF13812	403 - 459	7.6e-10	Pentatricopeptide repeat domain
PPR_1	PF12854	410 - 443	2.8e-08	PPR repeat
PPR_2	PF13041	414 - 463	4.5e-16	PPR repeat family
PPR_long	PF17177	453 - 600	7.2e-11	Pentacotripeptide-repeat region of PRORP
PPR_1	PF12854	483 - 514	5.7e-06	PPR repeat
PPR_3	PF13812	483 - 529	4.1e-06	Pentatricopeptide repeat domain
PPR_2	PF13041	487 - 533	4e-18	PPR repeat family
PPR	PF01535	489 - 518	4e-07	PPR repeat
PPR_3	PF13812	509 - 566	4.8e-12	Pentatricopeptide repeat domain
PPR_1	PF12854	516 - 549	1.9e-10	PPR repeat
PPR	PF01535	523 - 553	3.2e-07	PPR repeat
PPR_2	PF13041	528 - 569	1.3e-13	PPR repeat family
PPR_1	PF12854	551 - 583	6.2e-10	PPR repeat
PPR_2	PF13041	555 - 603	3.4e-15	PPR repeat family
PPR	PF01535	558 - 587	1.3e-07	PPR repeat

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Orthologous Genes (Group: OG0004844)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_2g22260
malus_domestica	MD05G1203800.v1.1
prunus_persica	Prupe.8G230100_v2.0.a1
pyrus_communis	pycom05g19020
rosa_chinensis	RchiOBHm_Chr6g0289191 RchiOBHm_Chr6g0289221
rosa_laevigata	RLG00000009132 RLG00000009134 RLG00000012320 RLG00000012323 RLG00000012325
rosa_multiflora	Rmu_co8489247.1_g000002 Rmu_sc0001527.1_g000006 Rmu_sc0001527.1_g000010 Rmu_sc0001527.1_g000011
rosa_roxburghii	Rroxscaffold_7G00179440 Rroxscaffold_7G00179500
rosa_rugosa	Rorug06G0201500 Rorug06G0201800 Rorug06G0201900 Rorug06G0202000
rosa_samantha	Rh6AG312700 Rh6AG312900 Rh6BG319700 Rh6BG320300 Rh6BG320400 Rh6CG326100 Rh6CG326400 Rh6DG312100
rosa_wichuraiana	Rw6G027020 Rw6G027040

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	3 cut(s)	483, 1568, 1680
Acc36I	ACCTGC	1 cut(s)	1067
Acc65I	GGTACC	1 cut(s)	1555
AccB1I	GGYRCC	1 cut(s)	1555
AciI	CCGC	4 cut(s)	253, 915, 956, 1225
AclWI	GGATC	1 cut(s)	1216
AcsI	RAATTY	4 cut(s)	64, 187, 1464, 1888
AfaI	GTAC	4 cut(s)	613, 860, 1014, 1557
AfiI	CCNNNNNNNGG	2 cut(s)	433, 1558
AjnI	CCWGG	1 cut(s)	1551
Alw21I	GWGCWC	2 cut(s)	844, 1663
AlwI	GGATC	1 cut(s)	1216
AlwNI	CAGNNNCTG	2 cut(s)	1312, 1446
AoxI	GGCC	3 cut(s)	285, 1238, 1549
ApeKI	GCWGC	2 cut(s)	1440, 1443
ApoI	RAATTY	4 cut(s)	64, 187, 1464, 1888
ArsI	GACNNNNNNTTYG	3 cut(s)	194, 226, 1880
AseI	ATTAAT	2 cut(s)	621, 1482
Asp700I	GAANNNNTTC	3 cut(s)	237, 1419, 1737
Asp718I	GGTACC	1 cut(s)	1555
AspS9I	GGNCC	1 cut(s)	1522
AsuHPI	GGTGA	3 cut(s)	946, 1758, 1894
AvaII	GGWCC	1 cut(s)	1522
BanI	GGYRCC	1 cut(s)	1555
BanII	GRGCYC	1 cut(s)	1596
Bbv12I	GWGCWC	2 cut(s)	844, 1663
BbvCI	CCTCAGC	1 cut(s)	1878
BbvI	GCAGC	2 cut(s)	1452, 1455
BccI	CCATC	3 cut(s)	755, 898, 1055
BciT130I	CCWGG	1 cut(s)	1553
BciVI	GTATCC	1 cut(s)	1163
BclI	TGATCA	2 cut(s)	573, 1161
BfaI	CTAG	2 cut(s)	648, 950
BfmI	CTRYAG	1 cut(s)	1245
BfuAI	ACCTGC	1 cut(s)	1067
BfuI	GTATCC	1 cut(s)	1163
BisI	GCNGC	3 cut(s)	957, 1441, 1444
BlsI	GCNGC	3 cut(s)	958, 1442, 1445
Bme1390I	CCNGG	1 cut(s)	1553
Bme18I	GGWCC	1 cut(s)	1522
BmgT120I	GGNCC	1 cut(s)	1522
BmiI	GGNNCC	2 cut(s)	1523, 1557
BmrFI	CCNGG	1 cut(s)	1553
BmrI	ACTGGG	1 cut(s)	153
BmsI	GCATC	4 cut(s)	363, 433, 1291, 1314
BmuI	ACTGGG	1 cut(s)	153
Bpu10I	CCTNAGC	2 cut(s)	681, 1878
BsaWI	WCCGGW	1 cut(s)	1558
BsaXI	ACNNNNNCTCC	2 cut(s)	139, 169
Bsc4I	CCNNNNNNNGG	2 cut(s)	433, 1558
Bse1I	ACTGG	4 cut(s)	159, 1276, 1844, 1860
Bse3DI	GCAATG	2 cut(s)	910, 1416
BseBI	CCWGG	1 cut(s)	1553
BseGI	GGATG	4 cut(s)	766, 1306, 1567, 1819
BseLI	CCNNNNNNNGG	2 cut(s)	433, 1558
BseMI	GCAATG	2 cut(s)	910, 1416
BseMII	CTCAG	3 cut(s)	1311, 1346, 1869
BseNI	ACTGG	4 cut(s)	159, 1276, 1844, 1860
BseRI	GAGGAG	1 cut(s)	1245
BseXI	GCAGC	2 cut(s)	1452, 1455
BsgI	GTGCAG	1 cut(s)	652
BshFI	GGCC	3 cut(s)	287, 1240, 1551
BshNI	GGYRCC	1 cut(s)	1555
BsiHKAI	GWGCWC	2 cut(s)	844, 1663
BsiSI	CCGG	1 cut(s)	1559
BslFI	GGGAC	1 cut(s)	1535
BslI	CCNNNNNNNGG	2 cut(s)	433, 1558
BsmFI	GGGAC	1 cut(s)	1535
BsnI	GGCC	3 cut(s)	287, 1240, 1551
Bsp1286I	GDGCHC	3 cut(s)	844, 1596, 1663
Bsp143I	GATC	3 cut(s)	573, 1161, 1221
BspACI	CCGC	4 cut(s)	253, 915, 956, 1225
BspANI	GGCC	3 cut(s)	287, 1240, 1551
BspCNI	CTCAG	3 cut(s)	1312, 1345, 1870
BspLI	GGNNCC	2 cut(s)	1523, 1557
BspMI	ACCTGC	1 cut(s)	1067
BspPI	GGATC	1 cut(s)	1216
BspT107I	GGYRCC	1 cut(s)	1555
BsrDI	GCAATG	2 cut(s)	910, 1416
BsrI	ACTGG	4 cut(s)	159, 1276, 1844, 1860
BssMI	GATC	3 cut(s)	573, 1161, 1221
Bst2UI	CCWGG	1 cut(s)	1553
Bst4CI	ACNGT	4 cut(s)	598, 1246, 1294, 1655
BstC8I	GCNNGC	1 cut(s)	20
BstDEI	CTNAG	8 cut(s)	324, 659, 681, 793, 1320, 1332, 1454, 1878
BstF5I	GGATG	4 cut(s)	766, 1306, 1567, 1819
BstKTI	GATC	3 cut(s)	576, 1164, 1224
BstMBI	GATC	3 cut(s)	573, 1161, 1221
BstMWI	GCNNNNNNNGC	2 cut(s)	452, 887
BstNI	CCWGG	1 cut(s)	1553
BstSCI	CCNGG	1 cut(s)	1551
BstSFI	CTRYAG	1 cut(s)	1245
BstV1I	GCAGC	2 cut(s)	1452, 1455
BsuI	GTATCC	1 cut(s)	1163
BsuRI	GGCC	3 cut(s)	287, 1240, 1551
BtsCI	GGATG	4 cut(s)	766, 1306, 1567, 1819
BtsI	GCAGTG	2 cut(s)	558, 997
BtsIMutI	CAGTG	4 cut(s)	166, 558, 590, 997
BveI	ACCTGC	1 cut(s)	1067
Cac8I	GCNNGC	1 cut(s)	20
CaiI	CAGNNNCTG	2 cut(s)	1312, 1446
Cfr13I	GGNCC	1 cut(s)	1522
Csp6I	GTAC	4 cut(s)	612, 859, 1013, 1556
CviQI	GTAC	4 cut(s)	612, 859, 1013, 1556
DdeI	CTNAG	8 cut(s)	324, 659, 681, 793, 1320, 1332, 1454, 1878
DpnI	GATC	3 cut(s)	575, 1163, 1223
DpnII	GATC	3 cut(s)	573, 1161, 1221
Eco147I	AGGCCT	1 cut(s)	1551
Eco24I	GRGCYC	1 cut(s)	1596
Eco47I	GGWCC	1 cut(s)	1522
EcoO109I	RGGNCCY	1 cut(s)	1522
EcoRII	CCWGG	1 cut(s)	1551
EcoT38I	GRGCYC	1 cut(s)	1596
FalI	AAGNNNNNCTT	3 cut(s)	38, 40, 72
FaqI	GGGAC	1 cut(s)	1535
FbaI	TGATCA	2 cut(s)	573, 1161
Fnu4HI	GCNGC	3 cut(s)	957, 1441, 1444
FokI	GGATG	4 cut(s)	773, 1313, 1574, 1826
FriOI	GRGCYC	1 cut(s)	1596
Fsp4HI	GCNGC	3 cut(s)	957, 1441, 1444
FspBI	CTAG	2 cut(s)	648, 950
GluI	GCNGC	3 cut(s)	957, 1441, 1444
HaeIII	GGCC	3 cut(s)	287, 1240, 1551
HapII	CCGG	1 cut(s)	1559
HincII	GTYRAC	2 cut(s)	363, 1133
HindII	GTYRAC	2 cut(s)	363, 1133
HindIII	AAGCTT	3 cut(s)	20, 74, 1004
HinfI	GANTC	4 cut(s)	259, 814, 1139, 1585
HpaI	GTTAAC	1 cut(s)	363
HpaII	CCGG	1 cut(s)	1559
HphI	GGTGA	3 cut(s)	946, 1758, 1894
Hpy166II	GTNNAC	6 cut(s)	311, 363, 1015, 1133, 1210, 1451
Hpy188I	TCNGA	3 cut(s)	846, 1335, 1827
Hpy188III	TCNNGA	6 cut(s)	37, 182, 821, 1031, 1298, 1807
Hpy8I	GTNNAC	6 cut(s)	311, 363, 1015, 1133, 1210, 1451
HpyAV	CCTTC	4 cut(s)	963, 1598, 1640, 1804
HpyCH4III	ACNGT	4 cut(s)	598, 1246, 1294, 1655
HpyCH4IV	ACGT	3 cut(s)	938, 1253, 1338
HpyF10VI	GCNNNNNNNGC	2 cut(s)	452, 887
HpyF3I	CTNAG	8 cut(s)	324, 659, 681, 793, 1320, 1332, 1454, 1878
HpySE526I	ACGT	3 cut(s)	938, 1253, 1338
KpnI	GGTACC	1 cut(s)	1559
Ksp22I	TGATCA	2 cut(s)	573, 1161
KspAI	GTTAAC	1 cut(s)	363
Kzo9I	GATC	3 cut(s)	573, 1161, 1221
LmnI	GCTCC	1 cut(s)	1666
Lsp1109I	GCAGC	2 cut(s)	1452, 1455
LweI	GCATC	4 cut(s)	363, 433, 1291, 1314
MaeI	CTAG	2 cut(s)	648, 950
MaeII	ACGT	3 cut(s)	938, 1253, 1338
MalI	GATC	3 cut(s)	575, 1163, 1223
MboI	GATC	3 cut(s)	573, 1161, 1221
MboII	GAAGA	6 cut(s)	82, 114, 360, 683, 809, 1730
MfeI	CAATTG	1 cut(s)	1868
MhlI	GDGCHC	3 cut(s)	844, 1596, 1663
MmeI	TCCRAC	4 cut(s)	543, 1353, 1731, 1871
MroXI	GAANNNNTTC	3 cut(s)	237, 1419, 1737
MseI	TTAA	9 cut(s)	362, 399, 516, 621, 1086, 1268, 1281, 1470, 1482
MslI	CAYNNNNRTG	1 cut(s)	1903
MspA1I	CMGCKG	1 cut(s)	1446
MspI	CCGG	1 cut(s)	1559
MspR9I	CCNGG	1 cut(s)	1553
MunI	CAATTG	1 cut(s)	1868
MvaI	CCWGG	1 cut(s)	1553
MwoI	GCNNNNNNNGC	2 cut(s)	452, 887
NdeII	GATC	3 cut(s)	573, 1161, 1221
NlaIV	GGNNCC	2 cut(s)	1523, 1557
NmuCI	GTSAC	5 cut(s)	934, 1672, 1764, 1882, 1896
PceI	AGGCCT	1 cut(s)	1551
PdmI	GAANNNNTTC	3 cut(s)	237, 1419, 1737
PfeI	GAWTC	4 cut(s)	259, 814, 1139, 1585
PkrI	GCNGC	3 cut(s)	958, 1442, 1445
PpuMI	RGGWCCY	1 cut(s)	1522
PshBI	ATTAAT	2 cut(s)	621, 1482
PsiI	TTATAA	3 cut(s)	483, 1568, 1680
Psp5II	RGGWCCY	1 cut(s)	1522
Psp6I	CCWGG	1 cut(s)	1551
PspGI	CCWGG	1 cut(s)	1551
PspN4I	GGNNCC	2 cut(s)	1523, 1557
PspPI	GGNCC	1 cut(s)	1522
PspPPI	RGGWCCY	1 cut(s)	1522
PstNI	CAGNNNCTG	2 cut(s)	1312, 1446
PvuII	CAGCTG	1 cut(s)	1446
RsaI	GTAC	4 cut(s)	613, 860, 1014, 1557
RsaNI	GTAC	4 cut(s)	612, 859, 1013, 1556
RseI	CAYNNNNRTG	1 cut(s)	1903
SaqAI	TTAA	9 cut(s)	362, 399, 516, 621, 1086, 1268, 1281, 1470, 1482
SatI	GCNGC	3 cut(s)	957, 1441, 1444
Sau3AI	GATC	3 cut(s)	573, 1161, 1221
Sau96I	GGNCC	1 cut(s)	1522
ScrFI	CCNGG	1 cut(s)	1553
SduI	GDGCHC	3 cut(s)	844, 1596, 1663
SfaNI	GCATC	4 cut(s)	363, 433, 1291, 1314
SfcI	CTRYAG	1 cut(s)	1245
SinI	GGWCC	1 cut(s)	1522
SmiMI	CAYNNNNRTG	1 cut(s)	1903
SseBI	AGGCCT	1 cut(s)	1551
SsiI	CCGC	4 cut(s)	253, 915, 956, 1225
SspI	AATATT	3 cut(s)	403, 1368, 1419
SspMI	CTAG	2 cut(s)	648, 950
StuI	AGGCCT	1 cut(s)	1551
StyD4I	CCNGG	1 cut(s)	1551
TaaI	ACNGT	4 cut(s)	598, 1246, 1294, 1655
TaiI	ACGT	3 cut(s)	941, 1256, 1341
TaqI	TCGA	3 cut(s)	1509, 1614, 1808
TatI	WGTACW	1 cut(s)	858
TauI	GCSGC	1 cut(s)	959
TfiI	GAWTC	4 cut(s)	259, 814, 1139, 1585
Tru1I	TTAA	9 cut(s)	362, 399, 516, 621, 1086, 1268, 1281, 1470, 1482
Tru9I	TTAA	9 cut(s)	362, 399, 516, 621, 1086, 1268, 1281, 1470, 1482
TscAI	CASTG	4 cut(s)	166, 558, 590, 997
TseFI	GTSAC	5 cut(s)	934, 1672, 1764, 1882, 1896
TseI	GCWGC	2 cut(s)	1440, 1443
Tsp45I	GTSAC	5 cut(s)	934, 1672, 1764, 1882, 1896
TspGWI	ACGGA	1 cut(s)	1516
TspRI	CASTG	4 cut(s)	166, 558, 590, 997
VpaK11BI	GGWCC	1 cut(s)	1522
VspI	ATTAAT	2 cut(s)	621, 1482
XapI	RAATTY	4 cut(s)	64, 187, 1464, 1888
XmnI	GAANNNNTTC	3 cut(s)	237, 1419, 1737
XspI	CTAG	2 cut(s)	648, 950

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.