Basic Information

Type: gene

Biological Identity

Species

rosa_rugosa

Chromosome

GWHBQTZ00000006

Orthogroup ID

OG0004844

Physical Location & Seq

Genomic Coordinates Forward (+)

32411022 .. 32414478

Gene Structure 3457 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rorug06G0201800.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3174 bp

ATGTCCAAACTAACCTTGGTCTCCTCTCTGTCCCCTGATGGCCAAGCTCTTCTCTCCCTTCTTCCTGCAAAGCAATCTTCCTCTGTACTCTCCTCATGGAACCCATCAAGCCAGACACCATGTTCATGGCAAGGAATCACATGCTCACCACAAAACAGAGTCATCTCCCTCTCTCTCCCCAACGTTTTCCTCAACCTCTCCTCTTTACCCCCACAGCTCTCCTCTCTCTCATCTCTCCAGCTTCTCAATCTCTCCTCCACCAACATTTCAGGCACTATCCCACCTTCCTTTGGACAACTTACTTATCTTCGCCTCCTCGACCTCTCCTCCAACTCTCTGTTTGGTCCGATTCCTCCAGAACTAGGCGCTCTCTCTGCTCTCCAGTTCCTCTTCTTGAACTCAAACAGATTGTCAGATAAAATCCCTCAACAACTTGCCAACCTCACTTCACTTCAAGTCCTTTGTCTCCAAGACAATCTCATCAACGGCTCGATACCCTCCCAACTCAGTTCTTTGGCTTCCCTCCAACAGTTTCGTGTTGGTGGGAATCCATATATAACCGGAGAAATTCCTTCCCAGTTGGGACTACTCACCAATCTCACCACATTCGGAGCTGCGGCTACTGGACTTTCCGGGACTATTCCACCCACATTTGGAAACTTGATCAATCTTCAGACACTGGCGCTCTATGATACTGAAATAGTTGGTTCAATACCACCCGAAATCGGGCTTTGTTCTGAGCTGAGGAACTTGTACCTGCACATGAACAAGCTCACTGGCTCCATTCCTCCACAATTAGGTAAGCTGCAAAAGCTCACCAGCCTGCTTGTTTGGGGAAATGACTTATCAGGATCAATCCCCGCTGAGATTTCCAACTGTTCCTCACTTGTTGTTCTCGATGCTTCTGCAAATGATCTCACTGGAGAGATTCCAAGAGACTTGGGGAAGCTGGTGTTCCTTGAACAGCTTCACCTATCGGATAACTCGCTTACAGGGTCGATTCCATCGCAGCTCAGCAACTGTAGCAGCCTTACAGCTCTCCAGCTCGACAAGAATCAGTTATCAGGGACAATTCCATGGCAGGTTGGTAATCTGAAATACTTGCAGAGTTTCTTCTTGTGGGGCAATGCAGTGTCTGGAACTATTCCTGCCTCGTTTGGGAACTGCACTGAACTGTATGCACTTGATCTTTCGAGAAACAAGCTCACCGGGTCAATACCAGAAGAGATTTTCTGTTTGAAGAGACTGAGCAAGCTTTTGCTTCTGGGAAATTCTTTATCCGGCAGGTTGCCACCTAGTGTAGCAAAATGTCAATCTCTAGTGCGGCTGAGACTCGGTGAGAACCAGCTTTCAGGACAAATTCCAAAGGAGATAGGCCAACTGCAAAACCTTGTGTTCCTTGACTTGTACATGAATCATTTCTCTGGAGGCCTTCCCATTGAGATTGCCAACATCACAGTTCTTGAGCTACTGGATGTGCACAATAACTACATTGGTGGCGAAATCCCATTTCAGTTTGGGGAGCTCGTAAATTTGGAGCAGCTTGATCTGAGCCGAAACAGCTTCAAGGGTGAGATTCCTTGGAGCTTTGGGAACTTGAGTTACTTGAACAAGCTCATCATCAACAACAATCTTCTCACAGGTTCAATACCAAAGTCTTTTCGAAATTTGCAGAAGCTGACGTTGTTGGATTTGAGCTTCAACAGCCTCTCTGGTGCAATCCCATCTGAAATCAGTCATGTGACAAGCTTGCAAATCAGTTTGGACTTGAGTTCCAATGGTTTTACAGGAGAACTTCCGGAGACAATGGCGGCTTTGACACAGTTACAGTGTCTGGATCTTTCCCATAATATGCTCTTTGGGAAAATTAAGGTTCTTGGCTCTCTAACTAGTCTCGCTTCCCTTAATATCTCCTGCAATGATTTCTCAGGTGCTATCCCAATAACCACCCCGTTCTTCAGAACTCTCTCCTCAAATTCTTACCTTCAAAATCCACATCTTTGTGAGTCTAATGATGGCTCTACTTGCTCTTCAAGTCTAATGCGGAAGAATGGCTTGAAATCTTCGAAAACTATTGCTTTAATTTCTGTGATTCTCTCTTCAGTAACTATAGCAGTTGTTGCTTCATGGATTCTTGTGATGCGAAATCATAGATATATGGTAGAGAAATCTTTAGGAGCATTAGCATCCTCGTCGGGGGCTGAAGATTTCTCCTATCCGTGGACTTTCATCCCATTCCAGAAGTTCAATTTCACTATTGACAACATCTTGGATTGTTTGAAAGAAGAAAATGTGATTGGAAAAGGATGTTCTGGGGTCGTCTACAAGGCAGAAACGCAAACCGGAGAGCTAATTGCGGTGAAAAAGTTGTGGAAGACAAAGCGAGAGGAAGAACCAATAGACTCTTTCGCTGCAGAGATTCAAATTCTTGGACACATTCGCCACCGGAACATTGTGAAGCTCCTAGGGTACTGTTCAAATAGAAGTGTCAAGCTACTTCTTTACAACTTCATTCCCAATGGCAATCTACAACAACTATTGCAAGACAATAGGAACTTGGACTGGGAAACGAGGTACAAGATTGCTATTGGGTCAGCTCAGGGTTTGGCATACCTTCATCATGATTGTGTCCCAGCAATTCTTCACAGGGATGTGAAGTGCAATAACATATTACTAGATTCCAAATGCGAAGCTTATCTTGCAGATTTTGGTCTTGCGAAATTGATGAACTCCCCAACTTACCATCATGCTATGTCCAGAGTTGCTGGCTCTTATGGTTACATTGCACCAGAGTATGGATACACAATGAACATAACAGAGAAGAGTGATGTCTACAGCTATGGTGTGGTTTTGCTAGAGATCCTAAGCGGACGTAATGCCGTTCAGCCCCAGATTGGTGATGGACTACACATTGTTGAATGGGTGAAGAAGAAGATGGGAAGTTTCGAACCAGCTTTATCAGTACTAGATACAAAGCTCCAAGGCCTGCCAGATCAAATGGTTCAAGAGATGCTGCAAACACTAGGAATAGCAATGTTTTGTGTCAACTCTTCGCCTGCAGAACGACCCACCATGAAGGAAGTGGTGGCATTGCTAATGGAGGTTAAGAGCCAACCTGAAGAATGGGGAAAAACTTCCCAACCCCTAATAAAGCAGTCTTCAAATCAAAGTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000959 GO:0000963 GO:0000966 GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004016 GO:0004518 GO:0004519 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0006139 GO:0006396 GO:0006397 GO:0006725 GO:0006807 GO:0007275 GO:0008150 GO:0008152 GO:0009451 GO:0009791 GO:0009845 GO:0009975 GO:0009987 GO:0010467 GO:0016070 GO:0016071 GO:0016556 GO:0016787 GO:0016788 GO:0016829 GO:0016849 GO:0032501 GO:0032502 GO:0034641 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044424 GO:0044444 GO:0044464 GO:0046483 GO:0048856 GO:0071704 GO:0080156 GO:0090304 GO:0090305 GO:0090351 GO:0090615 GO:0097159 GO:0140053 GO:1900864 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PPR PPR_1 PPR_2 PPR_3

Protein Analysis

1057

Amino Acids

115.21

Weight (kDa)

6.28

Isoelectric Point (pI)

43.71

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
LRRNT_2	PF08263	11 - 49	2.2e-09	Leucine rich repeat N-terminal domain
LRR_14	PF23598	67 - 203	7.1e-11	Leucine-rich repeat region
LRR_8	PF13855	368 - 426	7.3e-07	Leucine rich repeat
LRR_14	PF23598	482 - 565	9.6e-06	Leucine-rich repeat region
LRR_8	PF13855	535 - 594	1.6e-06	Leucine rich repeat
LRR_14	PF23598	555 - 639	3e-07	Leucine-rich repeat region
PK_Tyr_Ser-Thr	PF07714	761 - 1031	2.1e-43	Protein tyrosine and serine/threonine kinase
Pkinase	PF00069	762 - 1029	7.2e-45	Protein kinase domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0004844)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_2g22260
malus_domestica	MD05G1203800.v1.1
prunus_persica	Prupe.8G230100_v2.0.a1
pyrus_communis	pycom05g19020
rosa_chinensis	RchiOBHm_Chr6g0289191 RchiOBHm_Chr6g0289221
rosa_laevigata	RLG00000009132 RLG00000009134 RLG00000012320 RLG00000012323 RLG00000012325
rosa_multiflora	Rmu_co8489247.1_g000002 Rmu_sc0001527.1_g000006 Rmu_sc0001527.1_g000010 Rmu_sc0001527.1_g000011
rosa_roxburghii	Rroxscaffold_7G00179440 Rroxscaffold_7G00179500
rosa_rugosa	Rorug06G0201500 Rorug06G0201800 Rorug06G0201900 Rorug06G0202000
rosa_samantha	Rh6AG312700 Rh6AG312900 Rh6BG319700 Rh6BG320300 Rh6BG320400 Rh6CG326100 Rh6CG326400 Rh6DG312100
rosa_wichuraiana	Rw6G027020 Rw6G027040

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	3 cut(s)	765, 1072, 1275
AccB7I	CCANNNNNTGG	1 cut(s)	2795
AccI	GTMKAC	2 cut(s)	2322, 2832
AccIII	TCCGGA	1 cut(s)	1798
AciI	CCGC	7 cut(s)	617, 861, 1324, 1811, 2044, 2357, 2868
AclI	AACGTT	1 cut(s)	183
AclWI	GGATC	3 cut(s)	859, 1845, 2854
AcoI	YGGCCR	1 cut(s)	40
AcsI	RAATTY	7 cut(s)	567, 1270, 1359, 1531, 1666, 1975, 2424
AcuI	CTGAAG	5 cut(s)	656, 1942, 2085, 2223, 3138
AdeI	CACNNNGTG	1 cut(s)	1298
AfaI	GTAC	6 cut(s)	87, 755, 1409, 2471, 2576, 2964
AhlI	ACTAGT	1 cut(s)	1889
AjuI	GAANNNNNNNTTGG	2 cut(s)	2280, 2312
Alw21I	GWGCWC	2 cut(s)	1482, 1527
Alw26I	GTCTC	7 cut(s)	25, 470, 930, 1237, 1324, 1796, 1898
Alw44I	GTGCAC	1 cut(s)	1478
AlwI	GGATC	3 cut(s)	859, 1845, 2854
AlwNI	CAGNNNCTG	5 cut(s)	679, 1020, 1136, 1678, 1834
Aor13HI	TCCGGA	1 cut(s)	1798
AoxI	GGCC	4 cut(s)	40, 1375, 1429, 2983
ApaLI	GTGCAC	1 cut(s)	1478
ApeKI	GCWGC	7 cut(s)	614, 805, 1009, 1026, 1540, 2411, 3013
ApoI	RAATTY	7 cut(s)	567, 1270, 1359, 1531, 1666, 1975, 2424
ArsI	GACNNNNNNTTYG	2 cut(s)	395, 427
Asp700I	GAANNNNTTC	3 cut(s)	966, 1418, 3133
AspA2I	CCTAGG	1 cut(s)	2464
AspLEI	GCGC	2 cut(s)	368, 685
AspS9I	GGNCC	1 cut(s)	344
AsuC2I	CCSGG	2 cut(s)	634, 1210
AsuII	TTCGAA	3 cut(s)	1663, 2066, 2946
AvaII	GGWCC	1 cut(s)	344
AvrII	CCTAGG	1 cut(s)	2464
BaeGI	GKGCMC	1 cut(s)	1482
BalI	TGGCCA	1 cut(s)	42
BanII	GRGCYC	1 cut(s)	1527
BbsI	GAAGAC	2 cut(s)	2381, 3150
Bbv12I	GWGCWC	2 cut(s)	1482, 1527
BbvCI	CCTCAGC	1 cut(s)	743
BbvI	GCAGC	7 cut(s)	601, 792, 1021, 1038, 1552, 2398, 3000
BccI	CCATC	8 cut(s)	32, 112, 1012, 1732, 2009, 2751, 2894, 2929
BceAI	ACGGC	2 cut(s)	502, 2864
BcgI	CGANNNNNNTGC	4 cut(s)	887, 921, 2175, 2209
BciVI	GTATCC	1 cut(s)	2792
BclI	TGATCA	1 cut(s)	663
BcnI	CCSGG	2 cut(s)	634, 1210
BcoDI	GTCTC	7 cut(s)	25, 470, 930, 1237, 1324, 1796, 1898
BcuI	ACTAGT	1 cut(s)	1889
BfaI	CTAG	9 cut(s)	362, 1296, 1319, 1890, 2465, 2675, 2855, 2966, 3023
BfmI	CTRYAG	5 cut(s)	1021, 2109, 2412, 2833, 3057
BfoI	RGCGCY	2 cut(s)	369, 686
BfuAI	ACCTGC	3 cut(s)	765, 1072, 1275
BfuI	GTATCC	1 cut(s)	2792
BlnI	CCTAGG	1 cut(s)	2464
BlpI	GCTNAGC	1 cut(s)	1013
BmcAI	AGTACT	1 cut(s)	2964
Bme1390I	CCNGG	2 cut(s)	634, 1210
Bme18I	GGWCC	1 cut(s)	344
BmgT120I	GGNCC	1 cut(s)	344
BmiI	GGNNCC	2 cut(s)	101, 781
BmrFI	CCNGG	2 cut(s)	634, 1210
BmrI	ACTGGG	2 cut(s)	571, 2572
BmsI	GCATC	4 cut(s)	889, 2130, 2195, 3000
BmuI	ACTGGG	2 cut(s)	571, 2572
BpiI	GAAGAC	2 cut(s)	2381, 3150
BpmI	CTGGAG	6 cut(s)	221, 339, 365, 942, 1025, 1446
Bpu10I	CCTNAGC	3 cut(s)	743, 2598, 2864
Bpu1102I	GCTNAGC	1 cut(s)	1013
Bpu14I	TTCGAA	3 cut(s)	1663, 2066, 2946
BpuEI	CTTGAG	3 cut(s)	1484, 1618, 1789
BpuMI	CCSGG	2 cut(s)	634, 1210
BsaI	GGTCTC	1 cut(s)	25
BsaJI	CCNNGG	6 cut(s)	15, 1076, 1580, 2219, 2464, 2980
BsaWI	WCCGGW	4 cut(s)	560, 1798, 2342, 2445
BsaXI	ACNNNNNCTCC	2 cut(s)	311, 341
Bse1I	ACTGG	8 cut(s)	382, 577, 628, 684, 781, 925, 1476, 2567
Bse3DI	GCAATG	5 cut(s)	1132, 1924, 2781, 3039, 3089
BseAI	TCCGGA	1 cut(s)	1798
BseDI	CCNNGG	6 cut(s)	15, 1076, 1580, 2219, 2464, 2980
BseGI	GGATG	5 cut(s)	1480, 2186, 2229, 2312, 2656
BseMI	GCAATG	5 cut(s)	1132, 1924, 2781, 3039, 3089
BseNI	ACTGG	8 cut(s)	382, 577, 628, 684, 781, 925, 1476, 2567
BseRI	GAGGAG	8 cut(s)	13, 82, 190, 211, 244, 305, 316, 1960
BseSI	GKGCMC	1 cut(s)	1482
BseXI	GCAGC	7 cut(s)	601, 792, 1021, 1038, 1552, 2398, 3000
BseYI	CCCAGC	1 cut(s)	2632
BsgI	GTGCAG	2 cut(s)	743, 1150
BshFI	GGCC	4 cut(s)	42, 1377, 1431, 2985
BsiHKAI	GWGCWC	2 cut(s)	1482, 1527
BsiSI	CCGG	7 cut(s)	561, 633, 1209, 1281, 1799, 2343, 2446
BslFI	GGGAC	5 cut(s)	16, 597, 649, 1081, 2615
BsmAI	GTCTC	7 cut(s)	25, 470, 930, 1237, 1324, 1796, 1898
BsmFI	GGGAC	5 cut(s)	16, 597, 649, 1081, 2615
BsnI	GGCC	4 cut(s)	42, 1377, 1431, 2985
Bso31I	GGTCTC	1 cut(s)	25
Bsp119I	TTCGAA	3 cut(s)	1663, 2066, 2946
Bsp1286I	GDGCHC	2 cut(s)	1482, 1527
Bsp13I	TCCGGA	1 cut(s)	1798
Bsp1407I	TGTACA	1 cut(s)	1407
Bsp143I	GATC	8 cut(s)	663, 851, 913, 1186, 1546, 1837, 2859, 2992
Bsp1720I	GCTNAGC	1 cut(s)	1013
Bsp19I	CCATGG	1 cut(s)	1076
BspACI	CCGC	7 cut(s)	617, 861, 1324, 1811, 2044, 2357, 2868
BspANI	GGCC	4 cut(s)	42, 1377, 1431, 2985
BspEI	TCCGGA	1 cut(s)	1798
BspHI	TCATGA	1 cut(s)	2620
BspLI	GGNNCC	2 cut(s)	101, 781
BspMAI	CTGCAG	2 cut(s)	2416, 3061
BspMI	ACCTGC	3 cut(s)	765, 1072, 1275
BspPI	GGATC	3 cut(s)	859, 1845, 2854
BspQI	GCTCTTC	2 cut(s)	54, 2035
BspT104I	TTCGAA	3 cut(s)	1663, 2066, 2946
BspTNI	GGTCTC	1 cut(s)	25
BsrDI	GCAATG	5 cut(s)	1132, 1924, 2781, 3039, 3089
BsrGI	TGTACA	1 cut(s)	1407
BsrI	ACTGG	8 cut(s)	382, 577, 628, 684, 781, 925, 1476, 2567
BssECI	CCNNGG	6 cut(s)	15, 1076, 1580, 2219, 2464, 2980
BssMI	GATC	8 cut(s)	663, 851, 913, 1186, 1546, 1837, 2859, 2992
BssT1I	CCWWGG	5 cut(s)	15, 1076, 1580, 2464, 2980
Bst4CI	ACNGT	8 cut(s)	531, 878, 1022, 1176, 1459, 1824, 1830, 2474
Bst6I	CTCTTC	8 cut(s)	54, 395, 1218, 1235, 2035, 2104, 2816, 3055
BstAPI	GCANNNNNTGC	1 cut(s)	2120
BstAUI	TGTACA	1 cut(s)	1407
BstBI	TTCGAA	3 cut(s)	1663, 2066, 2946
BstC8I	GCNNGC	6 cut(s)	824, 1253, 1751, 2767, 2987, 3057
BstDSI	CCRYGG	2 cut(s)	1076, 2219
BstF5I	GGATG	5 cut(s)	1480, 2186, 2229, 2312, 2656
BstH2I	RGCGCY	2 cut(s)	369, 686
BstHHI	GCGC	2 cut(s)	368, 685
BstKTI	GATC	8 cut(s)	666, 854, 916, 1189, 1549, 1840, 2862, 2995
BstMAI	GTCTC	7 cut(s)	25, 470, 930, 1237, 1324, 1796, 1898
BstMBI	GATC	8 cut(s)	663, 851, 913, 1186, 1546, 1837, 2859, 2992
BstMWI	GCNNNNNNNGC	8 cut(s)	374, 811, 1023, 1560, 1704, 2025, 2120, 2699
BstNSI	RCATGY	1 cut(s)	144
BstSCI	CCNGG	2 cut(s)	632, 1208
BstSFI	CTRYAG	5 cut(s)	1021, 2109, 2412, 2833, 3057
BstSLI	GKGCMC	1 cut(s)	1482
BstV1I	GCAGC	7 cut(s)	601, 792, 1021, 1038, 1552, 2398, 3000
BstV2I	GAAGAC	2 cut(s)	2381, 3150
BstX2I	RGATCY	2 cut(s)	1837, 2859
BstXI	CCANNNNNNTGG	1 cut(s)	126
BstYI	RGATCY	2 cut(s)	1837, 2859
BsuI	GTATCC	1 cut(s)	2792
BsuRI	GGCC	4 cut(s)	42, 1377, 1431, 2985
BtgI	CCRYGG	2 cut(s)	1076, 2219
BtgZI	GCGATG	1 cut(s)	990
BtsCI	GGATG	5 cut(s)	1480, 2186, 2229, 2312, 2656
BtsI	GCAGTG	1 cut(s)	1137
BtsIMutI	CAGTG	6 cut(s)	677, 774, 918, 1137, 1167, 1835
BveI	ACCTGC	3 cut(s)	765, 1072, 1275
Cac8I	GCNNGC	6 cut(s)	824, 1253, 1751, 2767, 2987, 3057
CaiI	CAGNNNCTG	5 cut(s)	679, 1020, 1136, 1678, 1834
CciI	TCATGA	1 cut(s)	2620
CfoI	GCGC	2 cut(s)	368, 685
Cfr13I	GGNCC	1 cut(s)	344
Csp6I	GTAC	6 cut(s)	86, 754, 1408, 2470, 2575, 2963
CviQI	GTAC	6 cut(s)	86, 754, 1408, 2470, 2575, 2963
DpnI	GATC	8 cut(s)	665, 853, 915, 1188, 1548, 1839, 2861, 2994
DpnII	GATC	8 cut(s)	663, 851, 913, 1186, 1546, 1837, 2859, 2992
DraIII	CACNNNGTG	1 cut(s)	1298
EaeI	YGGCCR	1 cut(s)	40
Eam1104I	CTCTTC	8 cut(s)	54, 395, 1218, 1235, 2035, 2104, 2816, 3055
EarI	CTCTTC	8 cut(s)	54, 395, 1218, 1235, 2035, 2104, 2816, 3055
Ecl136II	GAGCTC	1 cut(s)	1525
Eco130I	CCWWGG	5 cut(s)	15, 1076, 1580, 2464, 2980
Eco147I	AGGCCT	2 cut(s)	1431, 2985
Eco24I	GRGCYC	1 cut(s)	1527
Eco31I	GGTCTC	1 cut(s)	25
Eco47I	GGWCC	1 cut(s)	344
Eco53kI	GAGCTC	1 cut(s)	1525
Eco57I	CTGAAG	5 cut(s)	656, 1942, 2085, 2223, 3138
EcoICRI	GAGCTC	1 cut(s)	1525
EcoT14I	CCWWGG	5 cut(s)	15, 1076, 1580, 2464, 2980
EcoT38I	GRGCYC	1 cut(s)	1527
ErhI	CCWWGG	5 cut(s)	15, 1076, 1580, 2464, 2980
FalI	AAGNNNNNCTT	4 cut(s)	2039, 2071, 2682, 2714
FaqI	GGGAC	5 cut(s)	16, 597, 649, 1081, 2615
FauI	CCCGC	1 cut(s)	868
FbaI	TGATCA	1 cut(s)	663
FblI	GTMKAC	2 cut(s)	2322, 2832
FokI	GGATG	5 cut(s)	1487, 2173, 2216, 2319, 2663
FriOI	GRGCYC	1 cut(s)	1527
FspBI	CTAG	9 cut(s)	362, 1296, 1319, 1890, 2465, 2675, 2855, 2966, 3023
GlaI	GCGC	2 cut(s)	367, 684
GsaI	CCCAGC	1 cut(s)	2636
GsuI	CTGGAG	6 cut(s)	221, 339, 365, 942, 1025, 1446
HaeII	RGCGCY	2 cut(s)	369, 686
HaeIII	GGCC	4 cut(s)	42, 1377, 1431, 2985
HapII	CCGG	7 cut(s)	561, 633, 1209, 1281, 1799, 2343, 2446
HhaI	GCGC	2 cut(s)	368, 685
Hin6I	GCGC	2 cut(s)	366, 683
HinP1I	GCGC	2 cut(s)	366, 683
HincII	GTYRAC	1 cut(s)	3046
HindII	GTYRAC	1 cut(s)	3046
HindIII	AAGCTT	3 cut(s)	1253, 1747, 2691
HpaII	CCGG	7 cut(s)	561, 633, 1209, 1281, 1799, 2343, 2446
Hpy166II	GTNNAC	5 cut(s)	1480, 2223, 2323, 2833, 3046
Hpy8I	GTNNAC	5 cut(s)	1480, 2223, 2323, 2833, 3046
Hpy99I	CGWCG	1 cut(s)	2197
HpyAV	CCTTC	7 cut(s)	68, 294, 582, 1442, 1994, 2624, 3070
HpyCH4III	ACNGT	8 cut(s)	531, 878, 1022, 1176, 1459, 1824, 1830, 2474
HpyCH4IV	ACGT	3 cut(s)	183, 1682, 2872
HpyF10VI	GCNNNNNNNGC	8 cut(s)	374, 811, 1023, 1560, 1704, 2025, 2120, 2699
HpySE526I	ACGT	3 cut(s)	183, 1682, 2872
HspAI	GCGC	2 cut(s)	366, 683
Kpn2I	TCCGGA	1 cut(s)	1798
Ksp22I	TGATCA	1 cut(s)	663
Kzo9I	GATC	8 cut(s)	663, 851, 913, 1186, 1546, 1837, 2859, 2992
LguI	GCTCTTC	2 cut(s)	54, 2035
LmnI	GCTCC	8 cut(s)	611, 785, 1522, 1537, 1584, 2177, 2466, 2982
Lsp1109I	GCAGC	7 cut(s)	601, 792, 1021, 1038, 1552, 2398, 3000
LweI	GCATC	4 cut(s)	889, 2130, 2195, 3000
MaeI	CTAG	9 cut(s)	362, 1296, 1319, 1890, 2465, 2675, 2855, 2966, 3023
MaeII	ACGT	3 cut(s)	183, 1682, 2872
MaeIII	GTNAC	5 cut(s)	1601, 1741, 1824, 2104, 2777
MalI	GATC	8 cut(s)	665, 853, 915, 1188, 1548, 1839, 2861, 2994
MboI	GATC	8 cut(s)	663, 851, 913, 1186, 1546, 1837, 2859, 2992
MflI	RGATCY	2 cut(s)	1837, 2859
MhlI	GDGCHC	2 cut(s)	1482, 1527
MlsI	TGGCCA	1 cut(s)	42
MluNI	TGGCCA	1 cut(s)	42
MlyI	GAGTC	4 cut(s)	168, 1326, 2015, 2396
MmeI	TCCRAC	4 cut(s)	354, 550, 897, 1668
Mox20I	TGGCCA	1 cut(s)	42
MroI	TCCGGA	1 cut(s)	1798
MroXI	GAANNNNTTC	3 cut(s)	966, 1418, 3133
MscI	TGGCCA	1 cut(s)	42
MseI	TTAA	5 cut(s)	1869, 1905, 2081, 3105, 3172
MslI	CAYNNNNRTG	4 cut(s)	124, 2001, 2625, 2649
Msp20I	TGGCCA	1 cut(s)	42
MspA1I	CMGCKG	1 cut(s)	863
MspI	CCGG	7 cut(s)	561, 633, 1209, 1281, 1799, 2343, 2446
MspR9I	CCNGG	2 cut(s)	634, 1210
MwoI	GCNNNNNNNGC	8 cut(s)	374, 811, 1023, 1560, 1704, 2025, 2120, 2699
NciI	CCSGG	2 cut(s)	634, 1210
NcoI	CCATGG	1 cut(s)	1076
NdeII	GATC	8 cut(s)	663, 851, 913, 1186, 1546, 1837, 2859, 2992
NlaIV	GGNNCC	2 cut(s)	101, 781
NmuCI	GTSAC	1 cut(s)	1741
NspI	RCATGY	1 cut(s)	144
NspV	TTCGAA	3 cut(s)	1663, 2066, 2946
PagI	TCATGA	1 cut(s)	2620
PceI	AGGCCT	2 cut(s)	1431, 2985
PciSI	GCTCTTC	2 cut(s)	54, 2035
PdmI	GAANNNNTTC	3 cut(s)	966, 1418, 3133
PflMI	CCANNNNNTGG	1 cut(s)	2795
PfoI	TCCNGGA	1 cut(s)	632
PleI	GAGTC	4 cut(s)	167, 1326, 2014, 2396
PpsI	GAGTC	4 cut(s)	167, 1326, 2014, 2396
Psp124BI	GAGCTC	1 cut(s)	1527
Psp1406I	AACGTT	1 cut(s)	183
PspFI	CCCAGC	1 cut(s)	2632
PspN4I	GGNNCC	2 cut(s)	101, 781
PspPI	GGNCC	1 cut(s)	344
PsrI	GAACNNNNNNTAC	2 cut(s)	1586, 1618
PstI	CTGCAG	2 cut(s)	2416, 3061
PstNI	CAGNNNCTG	5 cut(s)	679, 1020, 1136, 1678, 1834
PsuI	RGATCY	2 cut(s)	1837, 2859
RsaI	GTAC	6 cut(s)	87, 755, 1409, 2471, 2576, 2964
RsaNI	GTAC	6 cut(s)	86, 754, 1408, 2470, 2575, 2963
RseI	CAYNNNNRTG	4 cut(s)	124, 2001, 2625, 2649
SacI	GAGCTC	1 cut(s)	1527
SapI	GCTCTTC	2 cut(s)	54, 2035
SaqAI	TTAA	5 cut(s)	1869, 1905, 2081, 3105, 3172
Sau3AI	GATC	8 cut(s)	663, 851, 913, 1186, 1546, 1837, 2859, 2992
Sau96I	GGNCC	1 cut(s)	344
ScaI	AGTACT	1 cut(s)	2964
SchI	GAGTC	4 cut(s)	168, 1326, 2015, 2396
ScrFI	CCNGG	2 cut(s)	634, 1210
SduI	GDGCHC	2 cut(s)	1482, 1527
SfaNI	GCATC	4 cut(s)	889, 2130, 2195, 3000
SfcI	CTRYAG	5 cut(s)	1021, 2109, 2412, 2833, 3057
SfuI	TTCGAA	3 cut(s)	1663, 2066, 2946
SinI	GGWCC	1 cut(s)	344
SmiMI	CAYNNNNRTG	4 cut(s)	124, 2001, 2625, 2649
SmlI	CTYRAG	3 cut(s)	1463, 1597, 1768
SmoI	CTYRAG	3 cut(s)	1463, 1597, 1768
SpeI	ACTAGT	1 cut(s)	1889
SseBI	AGGCCT	2 cut(s)	1431, 2985
SsiI	CCGC	7 cut(s)	617, 861, 1324, 1811, 2044, 2357, 2868
SspMI	CTAG	9 cut(s)	362, 1296, 1319, 1890, 2465, 2675, 2855, 2966, 3023
SstI	GAGCTC	1 cut(s)	1527
StuI	AGGCCT	2 cut(s)	1431, 2985
StyD4I	CCNGG	2 cut(s)	632, 1208
StyI	CCWWGG	5 cut(s)	15, 1076, 1580, 2464, 2980
TaaI	ACNGT	8 cut(s)	531, 878, 1022, 1176, 1459, 1824, 1830, 2474
TaiI	ACGT	3 cut(s)	186, 1685, 2875
TaqI	TCGA	9 cut(s)	318, 491, 897, 998, 1047, 1193, 1663, 2066, 2946
TatI	WGTACW	3 cut(s)	85, 1407, 2962
TauI	GCSGC	3 cut(s)	620, 1327, 1814
Tru1I	TTAA	5 cut(s)	1869, 1905, 2081, 3105, 3172
Tru9I	TTAA	5 cut(s)	1869, 1905, 2081, 3105, 3172
TscAI	CASTG	6 cut(s)	684, 781, 925, 1137, 1174, 1835
TseFI	GTSAC	1 cut(s)	1741
TseI	GCWGC	7 cut(s)	614, 805, 1009, 1026, 1540, 2411, 3013
Tsp45I	GTSAC	1 cut(s)	1741
TspGWI	ACGGA	1 cut(s)	2208
TspRI	CASTG	6 cut(s)	684, 781, 925, 1137, 1174, 1835
Van91I	CCANNNNNTGG	1 cut(s)	2795
VneI	GTGCAC	1 cut(s)	1478
VpaK11BI	GGWCC	1 cut(s)	344
XapI	RAATTY	7 cut(s)	567, 1270, 1359, 1531, 1666, 1975, 2424
XceI	RCATGY	1 cut(s)	144
XcmI	CCANNNNNNNNNTGG	3 cut(s)	13, 2897, 3079
XmaJI	CCTAGG	1 cut(s)	2464
XmiI	GTMKAC	2 cut(s)	2322, 2832
XmnI	GAANNNNTTC	3 cut(s)	966, 1418, 3133
XspI	CTAG	9 cut(s)	362, 1296, 1319, 1890, 2465, 2675, 2855, 2966, 3023
ZrmI	AGTACT	1 cut(s)	2964

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.