Rroxscaffold_7G00196510

G-type lectin S-receptor-like serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0014817
Physical Location & Seq
Reverse (-)
39514686 .. 39525087
10402 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00196510.1

Sequence Viewer

Length: 750 bp
ATGGCACGCAATGAGTACCACGTCGTTCGAAACCCGTGCAAAGCAATAACCTCATTTCACAATCCATTCCAACCCAAAGATTCGAATTCGGAGGAGATGCATCGCACTAACACGGTTCTTAGCCGAGGCTACACTTCTATAATAGTCATAGGCCCGGAAATCGCGAAGCCCCATGAGGTCGGCGTACGGAGGCATAGAATCCAAGGAACACCAACAAGACATGTACCGTACACACTTGCCGAGTACAACAAGACCGAAGAATATATGATGGGGAACCGCCGCGGTCGGGCCATCACTCGGGAGGTACCGAGTAACAACAAGCATATACGACGACGTACCGAGCTATTGCTCGACGACGGAACACGAATGCGTGATCGTCGGAATAGTACTGAAATGGCCATTGTCAGTTTTGATGGTGCTGTACTTGGACAAATTCCTGCAAGTATGTCATATCATCATGTTGGATCTTCTGGCAAGGCTGCCAAGAAGAAAAAGAAGGGGAAAGATGCAAAAGGTTATTCCCTCTACTTTTGGTTTGAATTGGATGCTGACAACTCAAATGGCGCCTACGAGAAAAAGTGCCTAAACAATTGTTCTTGCCAGGCTTATGCATTTGTAGATAAAATAGGGCTTCTGGTCTGGTCCAGAGACCTAATTGATATACAACAGTTTCCGGTTGGTGGAGAGAATCTGACAATCTGTATATCCGCCTATGCCATAAAAAGTCAGGGGATTACAGTTTGTGCATAA

Protein Analysis

249

Amino Acids

28.11

Weight (kDa)

9.48

Isoelectric Point (pI)

46.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PAN_2 PF08276 191 - 221 1.2e-07 PAN-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 304
AccB1I GGYRCC 2 cut(s) 304, 563
AccII CGCG 2 cut(s) 164, 282
AciI CCGC 4 cut(s) 277, 280, 282, 708
AclWI GGATC 1 cut(s) 472
AcoI YGGCCR 1 cut(s) 396
AcsI RAATTY 2 cut(s) 85, 432
AcyI GRCGYC 1 cut(s) 564
AfaI GTAC 9 cut(s) 17, 186, 225, 230, 245, 306, 337, 388, 423
AfiI CCNNNNNNNGG 3 cut(s) 286, 297, 680
AflIII ACRYGT 1 cut(s) 220
AgsI TTSAA 1 cut(s) 539
AjiI CACGTC 1 cut(s) 22
AjnI CCWGG 1 cut(s) 600
AluBI AGCT 1 cut(s) 343
AluI AGCT 1 cut(s) 343
Alw26I GTCTC 1 cut(s) 642
AlwI GGATC 1 cut(s) 472
Ama87I CYCGRG 1 cut(s) 297
AoxI GGCC 3 cut(s) 151, 288, 396
ApeKI GCWGC 1 cut(s) 479
ApoI RAATTY 2 cut(s) 85, 432
Asp718I GGTACC 1 cut(s) 304
AspLEI GCGC 1 cut(s) 566
AspS9I GGNCC 3 cut(s) 152, 288, 642
AsuC2I CCSGG 1 cut(s) 155
AsuII TTCGAA 2 cut(s) 28, 83
AvaI CYCGRG 1 cut(s) 297
AvaII GGWCC 1 cut(s) 642
BalI TGGCCA 1 cut(s) 398
BanI GGYRCC 2 cut(s) 304, 563
BbvI GCAGC 1 cut(s) 466
BccI CCATC 3 cut(s) 262, 299, 407
BcgI CGANNNNNNTGC 2 cut(s) 18, 52
BciT130I CCWGG 1 cut(s) 602
BcnI CCSGG 1 cut(s) 155
BcoDI GTCTC 1 cut(s) 642
BfoI RGCGCY 1 cut(s) 567
BisI GCNGC 2 cut(s) 280, 480
BlsI GCNGC 2 cut(s) 281, 481
BmcAI AGTACT 1 cut(s) 388
Bme1390I CCNGG 2 cut(s) 155, 602
Bme18I GGWCC 1 cut(s) 642
BmeT110I CYCGRG 1 cut(s) 297
BmgBI CACGTC 1 cut(s) 22
BmgT120I GGNCC 3 cut(s) 152, 288, 642
BmiI GGNNCC 3 cut(s) 275, 306, 565
BmrFI CCNGG 2 cut(s) 155, 602
BmsI GCATC 4 cut(s) 87, 109, 496, 535
Bpu14I TTCGAA 2 cut(s) 28, 83
BpuMI CCSGG 1 cut(s) 155
BsaHI GRCGYC 1 cut(s) 564
BsaI GGTCTC 1 cut(s) 642
BsaJI CCNNGG 3 cut(s) 124, 202, 280
BsaWI WCCGGW 1 cut(s) 673
Bsc4I CCNNNNNNNGG 3 cut(s) 286, 297, 680
Bse3DI GCAATG 1 cut(s) 16
BseBI CCWGG 1 cut(s) 602
BseDI CCNNGG 3 cut(s) 124, 202, 280
BseGI GGATG 1 cut(s) 550
BseLI CCNNNNNNNGG 3 cut(s) 286, 297, 680
BseMI GCAATG 1 cut(s) 16
BseRI GAGGAG 1 cut(s) 107
BseXI GCAGC 1 cut(s) 466
Bsh1236I CGCG 2 cut(s) 164, 282
Bsh1285I CGRYCG 1 cut(s) 286
BshFI GGCC 3 cut(s) 153, 290, 398
BshNI GGYRCC 2 cut(s) 304, 563
BsiEI CGRYCG 1 cut(s) 286
BsiHKCI CYCGRG 1 cut(s) 297
BsiSI CCGG 2 cut(s) 155, 674
BsiWI CGTACG 1 cut(s) 184
BslI CCNNNNNNNGG 3 cut(s) 286, 297, 680
BsmAI GTCTC 1 cut(s) 642
BsmI GAATGC 1 cut(s) 372
BsnI GGCC 3 cut(s) 153, 290, 398
Bso31I GGTCTC 1 cut(s) 642
BsoBI CYCGRG 1 cut(s) 297
Bsp119I TTCGAA 2 cut(s) 28, 83
Bsp143I GATC 2 cut(s) 373, 464
Bsp68I TCGCGA 1 cut(s) 164
BspACI CCGC 4 cut(s) 277, 280, 282, 708
BspANI GGCC 3 cut(s) 153, 290, 398
BspFNI CGCG 2 cut(s) 164, 282
BspLI GGNNCC 3 cut(s) 275, 306, 565
BspPI GGATC 1 cut(s) 472
BspT104I TTCGAA 2 cut(s) 28, 83
BspT107I GGYRCC 2 cut(s) 304, 563
BspTNI GGTCTC 1 cut(s) 642
BsrDI GCAATG 1 cut(s) 16
BssECI CCNNGG 3 cut(s) 124, 202, 280
BssMI GATC 2 cut(s) 373, 464
BssNI GRCGYC 1 cut(s) 564
BssT1I CCWWGG 1 cut(s) 202
Bst2UI CCWGG 1 cut(s) 602
Bst4CI ACNGT 4 cut(s) 115, 228, 669, 739
BstACI GRCGYC 1 cut(s) 564
BstBI TTCGAA 2 cut(s) 28, 83
BstC8I GCNNGC 1 cut(s) 7
BstDEI CTNAG 1 cut(s) 119
BstDSI CCRYGG 1 cut(s) 280
BstF5I GGATG 1 cut(s) 550
BstFNI CGCG 2 cut(s) 164, 282
BstH2I RGCGCY 1 cut(s) 567
BstHHI GCGC 1 cut(s) 566
BstKTI GATC 2 cut(s) 376, 467
BstMAI GTCTC 1 cut(s) 642
BstMBI GATC 2 cut(s) 373, 464
BstMCI CGRYCG 1 cut(s) 286
BstNI CCWGG 1 cut(s) 602
BstNSI RCATGY 1 cut(s) 224
BstSCI CCNGG 2 cut(s) 153, 600
BstUI CGCG 2 cut(s) 164, 282
BstV1I GCAGC 1 cut(s) 466
BstX2I RGATCY 1 cut(s) 464
BstYI RGATCY 1 cut(s) 464
BsuRI GGCC 3 cut(s) 153, 290, 398
BtgI CCRYGG 1 cut(s) 280
BtgZI GCGATG 1 cut(s) 86
BtrI CACGTC 1 cut(s) 22
BtsCI GGATG 1 cut(s) 550
BtuMI TCGCGA 1 cut(s) 164
Cac8I GCNNGC 1 cut(s) 7
CfoI GCGC 1 cut(s) 566
Cfr13I GGNCC 3 cut(s) 152, 288, 642
Cfr42I CCGCGG 1 cut(s) 283
Csp6I GTAC 9 cut(s) 16, 185, 224, 229, 244, 305, 336, 387, 422
CviAII CATG 3 cut(s) 173, 221, 458
CviQI GTAC 9 cut(s) 16, 185, 224, 229, 244, 305, 336, 387, 422
DdeI CTNAG 1 cut(s) 119
DinI GGCGCC 1 cut(s) 565
DpnI GATC 2 cut(s) 375, 466
DpnII GATC 2 cut(s) 373, 464
EaeI YGGCCR 1 cut(s) 396
EciI GGCGGA 1 cut(s) 697
Eco130I CCWWGG 1 cut(s) 202
Eco31I GGTCTC 1 cut(s) 642
Eco47I GGWCC 1 cut(s) 642
Eco88I CYCGRG 1 cut(s) 297
EcoRI GAATTC 1 cut(s) 85
EcoRII CCWGG 1 cut(s) 600
EcoT14I CCWWGG 1 cut(s) 202
EcoT22I ATGCAT 2 cut(s) 102, 613
EgeI GGCGCC 1 cut(s) 565
EheI GGCGCC 1 cut(s) 565
ErhI CCWWGG 1 cut(s) 202
FaeI CATG 3 cut(s) 176, 224, 461
FatI CATG 3 cut(s) 172, 220, 457
Fnu4HI GCNGC 2 cut(s) 280, 480
FokI GGATG 1 cut(s) 557
Fsp4HI GCNGC 2 cut(s) 280, 480
GlaI GCGC 1 cut(s) 565
GluI GCNGC 2 cut(s) 280, 480
HaeII RGCGCY 1 cut(s) 567
HaeIII GGCC 3 cut(s) 153, 290, 398
HapII CCGG 2 cut(s) 155, 674
HhaI GCGC 1 cut(s) 566
Hin1I GRCGYC 1 cut(s) 564
Hin1II CATG 3 cut(s) 176, 224, 461
Hin6I GCGC 1 cut(s) 564
HinP1I GCGC 1 cut(s) 564
HinfI GANTC 3 cut(s) 80, 198, 688
HpaII CCGG 2 cut(s) 155, 674
Hpy166II GTNNAC 1 cut(s) 231
Hpy188I TCNGA 3 cut(s) 91, 381, 693
Hpy188III TCNNGA 3 cut(s) 163, 299, 645
Hpy8I GTNNAC 1 cut(s) 231
Hpy99I CGWCG 6 cut(s) 26, 333, 336, 356, 359, 381
HpyAV CCTTC 1 cut(s) 490
HpyCH4III ACNGT 4 cut(s) 115, 228, 669, 739
HpyCH4IV ACGT 2 cut(s) 21, 334
HpyCH4V TGCA 6 cut(s) 39, 100, 440, 509, 611, 746
HpyF3I CTNAG 1 cut(s) 119
HpySE526I ACGT 2 cut(s) 21, 334
Hsp92I GRCGYC 1 cut(s) 564
Hsp92II CATG 3 cut(s) 176, 224, 461
HspAI GCGC 1 cut(s) 564
KasI GGCGCC 1 cut(s) 563
KpnI GGTACC 1 cut(s) 308
KspI CCGCGG 1 cut(s) 283
Kzo9I GATC 2 cut(s) 373, 464
Lsp1109I GCAGC 1 cut(s) 466
LweI GCATC 4 cut(s) 87, 109, 496, 535
MaeII ACGT 2 cut(s) 21, 334
MaeIII GTNAC 1 cut(s) 311
MalI GATC 2 cut(s) 375, 466
MboI GATC 2 cut(s) 373, 464
MboII GAAGA 3 cut(s) 269, 459, 499
MfeI CAATTG 1 cut(s) 589
MflI RGATCY 1 cut(s) 464
MlsI TGGCCA 1 cut(s) 398
MluCI AATT 5 cut(s) 85, 432, 539, 589, 654
MluNI TGGCCA 1 cut(s) 398
Mly113I GGCGCC 1 cut(s) 564
MmeI TCCRAC 3 cut(s) 94, 359, 442
MnlI CCTC 7 cut(s) 61, 85, 119, 169, 183, 295, 533
Mox20I TGGCCA 1 cut(s) 398
Mph1103I ATGCAT 2 cut(s) 102, 613
MscI TGGCCA 1 cut(s) 398
Msp20I TGGCCA 1 cut(s) 398
MspA1I CMGCKG 1 cut(s) 282
MspI CCGG 2 cut(s) 155, 674
MspR9I CCNGG 2 cut(s) 155, 602
MunI CAATTG 1 cut(s) 589
Mva1269I GAATGC 1 cut(s) 372
MvaI CCWGG 1 cut(s) 602
MvnI CGCG 2 cut(s) 164, 282
NarI GGCGCC 1 cut(s) 564
NciI CCSGG 1 cut(s) 155
NdeII GATC 2 cut(s) 373, 464
NlaIII CATG 3 cut(s) 176, 224, 461
NlaIV GGNNCC 3 cut(s) 275, 306, 565
NmeAIII GCCGAG 2 cut(s) 149, 265
NruI TCGCGA 1 cut(s) 164
NsiI ATGCAT 2 cut(s) 102, 613
NspI RCATGY 1 cut(s) 224
NspV TTCGAA 2 cut(s) 28, 83
PciI ACATGT 1 cut(s) 220
PctI GAATGC 1 cut(s) 372
PfeI GAWTC 3 cut(s) 80, 198, 688
Pfl23II CGTACG 1 cut(s) 184
PkrI GCNGC 2 cut(s) 281, 481
PluTI GGCGCC 1 cut(s) 567
PscI ACATGT 1 cut(s) 220
Psp6I CCWGG 1 cut(s) 600
PspGI CCWGG 1 cut(s) 600
PspLI CGTACG 1 cut(s) 184
PspN4I GGNNCC 3 cut(s) 275, 306, 565
PspPI GGNCC 3 cut(s) 152, 288, 642
PsuI RGATCY 1 cut(s) 464
RruI TCGCGA 1 cut(s) 164
RsaI GTAC 9 cut(s) 17, 186, 225, 230, 245, 306, 337, 388, 423
RsaNI GTAC 9 cut(s) 16, 185, 224, 229, 244, 305, 336, 387, 422
SacII CCGCGG 1 cut(s) 283
SatI GCNGC 2 cut(s) 280, 480
Sau3AI GATC 2 cut(s) 373, 464
Sau96I GGNCC 3 cut(s) 152, 288, 642
ScaI AGTACT 1 cut(s) 388
ScrFI CCNGG 2 cut(s) 155, 602
SetI ASST 8 cut(s) 24, 53, 180, 306, 337, 345, 517, 654
SfaNI GCATC 4 cut(s) 87, 109, 496, 535
SfoI GGCGCC 1 cut(s) 565
Sfr303I CCGCGG 1 cut(s) 283
SfuI TTCGAA 2 cut(s) 28, 83
SgrBI CCGCGG 1 cut(s) 283
SinI GGWCC 1 cut(s) 642
Sse9I AATT 5 cut(s) 85, 432, 539, 589, 654
SsiI CCGC 4 cut(s) 277, 280, 282, 708
SspDI GGCGCC 1 cut(s) 563
StyD4I CCNGG 2 cut(s) 153, 600
StyI CCWWGG 1 cut(s) 202
TaaI ACNGT 4 cut(s) 115, 228, 669, 739
TaiI ACGT 2 cut(s) 24, 337
TaqI TCGA 3 cut(s) 28, 83, 351
TaqII GACCGA 1 cut(s) 269
TasI AATT 5 cut(s) 85, 432, 539, 589, 654
TatI WGTACW 3 cut(s) 243, 386, 421
TauI GCSGC 1 cut(s) 282
TfiI GAWTC 3 cut(s) 80, 198, 688
TseI GCWGC 1 cut(s) 479
TspGWI ACGGA 2 cut(s) 202, 372
VpaK11BI GGWCC 1 cut(s) 642
XapI RAATTY 2 cut(s) 85, 432
XceI RCATGY 1 cut(s) 224
ZrmI AGTACT 1 cut(s) 388
Zsp2I ATGCAT 2 cut(s) 102, 613
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.