Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000007

Orthogroup ID

OG0003614

Physical Location & Seq

Genomic Coordinates Forward (+)

65297700 .. 65300783

Gene Structure 3084 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_7G00214640.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1932 bp

ATGCTAAGGCCTAATTGGTGTGATCAGAAGCATGAAGAAGAATTAGACTCTGAAGCAATATATAAGAACTTTGTTCTTCGGGTTATGGCAGCAAGAAGTCCGAATGTTAAACTTCGAAAAGCACTAAACAGAAAGTCGCCAAGTTTGAAATGCCCACTTACGGCTCCTGCAAAATCTTCTAAGCCTAGAGTTTCAAGGTTGGCCCTGATTTCTTCAATCTCTCATAAGATAGTTGATGCCAAATATAAGGCCAGACCTCTCCATACTAAGATCACTGCAACAACCCCAAATGTTAAGGCAAAGCAACCATCTTCTGTAGCCAAAGCTCTCACTACTCCAAGGAATAGAAAACCCATCTCAAATCCAGATACATTTCGGAGTGTTAGGAACTCCAAAGCCAAAAATGTTGTGGTGCCAAAGAATAGGATCGTGGCAAAAGCTTTGGTCTTCAACTCTCCAAAGAAGGCTGTGAAAACCAAGGGTTCATCGGAATGTGGTAGTAATGTGGGAAAACTTTGTGCAGCCATGAAGAAACTTGATATCACAAGTGGAAAGAAACATGTATTGGGGTATACCAAGACATTGCCTTTAGATACTCCAAGAAAACAATTGAGAGGGCGAGAGGTCAAAAGCCGGGTTTTTGATTCATTGCATTCTAACAAAGGTAAGGGGCAGGAGGCAAAGTCCTTGAAGAGGAAGAAAATGGAGAAAGATTTGAAGCAGTGTTATGATACAGGGCCTTATGAAGAGGTTGATAATGATCCCAGTGATGTGAAGGTTGAAGAAAAATCACGGAATGGTTCCTTGGAAGGTTTCTCCAAGTCAGTTGATTCTAAAAGTAATAAAGTAAATGGAGATGAGGAGCATTTGGAAGCTGGAAAAAGTTCAGAATCCTCTTTAAATGAGAATCAAGTTGAAGTGGTATCAGAAACTTCAAAAGCTGATATTAGCTCCTTGTCAAGTTCTGAAAAGAATGATTCGGAAGTGAACTGTGATAAGGAAAAGACCAACAATGGAAATGGACATGAAGATAAACCAAAGTTAAGTTCAGGGATGGGGAAGACCCTTGAACACATGGAGGAAGATGATAAAGAAAATGCTGTAGCCTCGGACAACACCGAACATGACAATGAGATCATGCATAAGGATGATAAAGAAAACCGTATAACTTCTGAAGATAACAGAGAACAAAAGCGTAATGGCTTGAAGAGGAAAAATCTTGGTAACCATAGTGCTTCCAAAAACTCACAAAAGGTCATTCAAGTAGCAGAAAAGATTCCCAAAGAAAACTCCACATCAGCTGCCACTAGTGCTCAAGGGGTCAAGTATAGTAAACCAAAACCCACAAATCCGAAGCCTTTTAGGTTCAGAACTGATGAAAGAGGGATGCTTAAGGAAGCAAACTTGGAGAAAAAGGTTCATGCACCTCTCAAGGAAATCACATTAGACACTCTGCCAGAAATGTCCACAAAAAAACATCAGAATGTGATACAGGGGAAGGTAGGAGCTACTTGCTTCAAGACTTCAAAAGGAGACTTAGAACGGAAGTTATCTGTAATGACTCCTCCACAAAGATGTAAAGTTTTGACCGCACAAAAACCACAGAAGGAAAGAACAAAGTCACCTATGGTGCAGCCAAGTTTTGCAAGACCAAGAGGGGTGGATTCAAGTAAGAAGAAGTCTGCCTCCAAGACACCTTGCCAACTCAGTATAATAAAGGAAAGCGTATCAACAAATATAAGACCCAGGAAAGCAGCAAAACCGTGTGGTGTTTCTTCAGCAACCAAAGCTCGTACTCCTTCTCGGTCATCATCACGGGGAAGAAGGCCTGCAACCATTCCAAAGGAGCCACACTTTCACACTATCCATGTACCGAAGGACTGTACAAGGCTGCAGAAGGAAAATGACTTTACTGATGCCCAACAAAACTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

No domains found.

Protein Analysis

643

Amino Acids

71.37

Weight (kDa)

9.89

Isoelectric Point (pI)

36.7

Instability Index

Protein Domains (Pfam)

No Pfam domains detected for this protein.

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003614)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G17000 AT4G17000 AT4G17000
fragaria_vesca	FvH4_2g02560 FvH4_2g02560 FvH4_2g02560 FvH4_2g02560 FvH4_2g02560
malus_domestica	MD05G1112300.v1.1 MD10G1114900.v1.1
prunus_persica	Prupe.8G156900_v2.0.a1 Prupe.8G157000_v2.0.a1
pyrus_communis	pycom02g05590 pycom05g07390 pycom05g10780 pycom07g10710 pycom12g10280 pycom16g18860
rosa_chinensis	RchiOBHm_Chr6g0246371 RchiOBHm_Chr6g0246451 RchiOBHm_Chr6g0246631 RchiOBHm_Chr6g0246641
rosa_laevigata	RLG00000000929 RLG00000015260
rosa_multiflora	Rmu_sc0001066.1_g000005 Rmu_sc0008965.1_g000002
rosa_roxburghii	Rroxscaffold_7G00214640
rosa_rugosa	Rorug05G0520500
rosa_samantha	Rh6AG034800 Rh6AG035100 Rh6BG029900 Rh6CG030300 Rh6DG029200
rosa_wichuraiana	Rw6G002820 Rw6G002980

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	1 cut(s)	412
AccI	GTMKAC	1 cut(s)	572
AciI	CCGC	1 cut(s)	1590
AclWI	GGATC	2 cut(s)	434, 755
AcuI	CTGAAG	3 cut(s)	72, 1194, 1761
AfaI	GTAC	3 cut(s)	1795, 1872, 1885
AfiI	CCNNNNNNNGG	2 cut(s)	160, 693
AflII	CTTAAG	1 cut(s)	1391
AflIII	ACRYGT	1 cut(s)	559
AhlI	ACTAGT	1 cut(s)	1307
AjnI	CCWGG	1 cut(s)	1745
AjuI	GAANNNNNNNTTGG	6 cut(s)	548, 580, 788, 820, 1030, 1062
AluBI	AGCT	8 cut(s)	326, 440, 875, 941, 951, 1301, 1508, 1790
AluI	AGCT	8 cut(s)	326, 440, 875, 941, 951, 1301, 1508, 1790
Alw21I	GWGCWC	1 cut(s)	1315
Alw26I	GTCTC	1 cut(s)	1527
AlwI	GGATC	2 cut(s)	434, 755
AoxI	GGCC	5 cut(s)	8, 201, 249, 737, 1826
ApeKI	GCWGC	6 cut(s)	89, 521, 1301, 1633, 1754, 1891
Asp700I	GAANNNNTTC	2 cut(s)	883, 1275
AspS9I	GGNCC	2 cut(s)	202, 737
AsuC2I	CCSGG	1 cut(s)	635
AsuHPI	GGTGA	1 cut(s)	1614
AsuII	TTCGAA	1 cut(s)	115
BanI	GGYRCC	1 cut(s)	412
BbsI	GAAGAC	2 cut(s)	439, 1067
Bbv12I	GWGCWC	1 cut(s)	1315
BbvI	GCAGC	6 cut(s)	101, 533, 1288, 1645, 1766, 1878
BccI	CCATC	3 cut(s)	316, 362, 1048
BceAI	ACGGC	1 cut(s)	177
BciT130I	CCWGG	1 cut(s)	1747
BclI	TGATCA	1 cut(s)	22
BcnI	CCSGG	1 cut(s)	635
BcoDI	GTCTC	1 cut(s)	1527
BcuI	ACTAGT	1 cut(s)	1307
BfaI	CTAG	2 cut(s)	186, 1308
BfmI	CTRYAG	3 cut(s)	315, 1101, 1892
BfrI	CTTAAG	1 cut(s)	1391
BisI	GCNGC	6 cut(s)	90, 522, 1302, 1634, 1755, 1892
BlsI	GCNGC	6 cut(s)	91, 523, 1303, 1635, 1756, 1893
Bme1390I	CCNGG	2 cut(s)	635, 1747
BmgT120I	GGNCC	2 cut(s)	202, 737
BmiI	GGNNCC	4 cut(s)	165, 414, 802, 1848
BmrFI	CCNGG	2 cut(s)	635, 1747
BmrI	ACTGGG	1 cut(s)	759
BmsI	GCATC	3 cut(s)	226, 1377, 1906
BmuI	ACTGGG	1 cut(s)	759
BpiI	GAAGAC	2 cut(s)	439, 1067
Bpu10I	CCTNAGC	1 cut(s)	5
Bpu14I	TTCGAA	1 cut(s)	115
BpuEI	CTTGAG	2 cut(s)	1299, 1415
BpuMI	CCSGG	1 cut(s)	635
BsaBI	GATNNNNATC	1 cut(s)	759
BsaJI	CCNNGG	5 cut(s)	338, 477, 804, 1107, 1745
BsaXI	ACNNNNNCTCC	2 cut(s)	668, 698
Bsc4I	CCNNNNNNNGG	2 cut(s)	160, 693
Bse1I	ACTGG	1 cut(s)	765
Bse3DI	GCAATG	2 cut(s)	581, 647
Bse8I	GATNNNNATC	1 cut(s)	759
BseBI	CCWGG	1 cut(s)	1747
BseDI	CCNNGG	5 cut(s)	338, 477, 804, 1107, 1745
BseGI	GGATG	3 cut(s)	1059, 1153, 1392
BseJI	GATNNNNATC	1 cut(s)	759
BseLI	CCNNNNNNNGG	2 cut(s)	160, 693
BseMI	GCAATG	2 cut(s)	581, 647
BseMII	CTCAG	1 cut(s)	1720
BseNI	ACTGG	1 cut(s)	765
BseRI	GAGGAG	2 cut(s)	875, 1554
BseXI	GCAGC	6 cut(s)	101, 533, 1288, 1645, 1766, 1878
BsgI	GTGCAG	2 cut(s)	540, 1652
BshFI	GGCC	5 cut(s)	10, 203, 251, 739, 1828
BshNI	GGYRCC	1 cut(s)	412
BsiHKAI	GWGCWC	1 cut(s)	1315
BsiSI	CCGG	1 cut(s)	634
BslI	CCNNNNNNNGG	2 cut(s)	160, 693
BsmAI	GTCTC	1 cut(s)	1527
BsmI	GAATGC	1 cut(s)	652
BsnI	GGCC	5 cut(s)	10, 203, 251, 739, 1828
Bsp119I	TTCGAA	1 cut(s)	115
Bsp1286I	GDGCHC	1 cut(s)	1315
Bsp1407I	TGTACA	1 cut(s)	1883
Bsp143I	GATC	5 cut(s)	22, 270, 426, 760, 1134
BspACI	CCGC	1 cut(s)	1590
BspANI	GGCC	5 cut(s)	10, 203, 251, 739, 1828
BspCNI	CTCAG	1 cut(s)	1719
BspLI	GGNNCC	4 cut(s)	165, 414, 802, 1848
BspMAI	CTGCAG	1 cut(s)	1896
BspPI	GGATC	2 cut(s)	434, 755
BspT104I	TTCGAA	1 cut(s)	115
BspT107I	GGYRCC	1 cut(s)	412
BspTI	CTTAAG	1 cut(s)	1391
BsrDI	GCAATG	2 cut(s)	581, 647
BsrGI	TGTACA	1 cut(s)	1883
BsrI	ACTGG	1 cut(s)	765
BssECI	CCNNGG	5 cut(s)	338, 477, 804, 1107, 1745
BssMI	GATC	5 cut(s)	22, 270, 426, 760, 1134
BssNAI	GTATAC	1 cut(s)	573
BssT1I	CCWWGG	3 cut(s)	338, 477, 804
Bst1107I	GTATAC	1 cut(s)	573
Bst2UI	CCWGG	1 cut(s)	1747
Bst4CI	ACNGT	4 cut(s)	992, 1163, 1764, 1883
Bst6I	CTCTTC	3 cut(s)	686, 741, 1202
BstAFI	CTTAAG	1 cut(s)	1391
BstAUI	TGTACA	1 cut(s)	1883
BstBI	TTCGAA	1 cut(s)	115
BstC8I	GCNNGC	1 cut(s)	1830
BstDEI	CTNAG	5 cut(s)	5, 180, 267, 1537, 1706
BstEII	GGTNACC	1 cut(s)	1223
BstENI	CCTNNNNNAGG	1 cut(s)	691
BstF5I	GGATG	3 cut(s)	1059, 1153, 1392
BstKTI	GATC	5 cut(s)	25, 273, 429, 763, 1137
BstMAI	GTCTC	1 cut(s)	1527
BstMBI	GATC	5 cut(s)	22, 270, 426, 760, 1134
BstMWI	GCNNNNNNNGC	2 cut(s)	1310, 1787
BstNI	CCWGG	1 cut(s)	1747
BstNSI	RCATGY	1 cut(s)	563
BstPI	GGTNACC	1 cut(s)	1223
BstSCI	CCNGG	2 cut(s)	633, 1745
BstSFI	CTRYAG	3 cut(s)	315, 1101, 1892
BstV1I	GCAGC	6 cut(s)	101, 533, 1288, 1645, 1766, 1878
BstV2I	GAAGAC	2 cut(s)	439, 1067
BstZ17I	GTATAC	1 cut(s)	573
BsuRI	GGCC	5 cut(s)	10, 203, 251, 739, 1828
BtsCI	GGATG	3 cut(s)	1059, 1153, 1392
BtsI	GCAGTG	2 cut(s)	273, 728
BtsIMutI	CAGTG	3 cut(s)	273, 728, 772
Cac8I	GCNNGC	1 cut(s)	1830
Cfr13I	GGNCC	2 cut(s)	202, 737
Csp6I	GTAC	3 cut(s)	1794, 1871, 1884
CspCI	CAANNNNNGTGG	2 cut(s)	1641, 1676
CviAII	CATG	9 cut(s)	32, 526, 560, 1025, 1075, 1124, 1138, 1421, 1868
CviQI	GTAC	3 cut(s)	1794, 1871, 1884
DdeI	CTNAG	5 cut(s)	5, 180, 267, 1537, 1706
DpnI	GATC	5 cut(s)	24, 272, 428, 762, 1136
DpnII	GATC	5 cut(s)	22, 270, 426, 760, 1134
DraI	TTTAAA	1 cut(s)	900
Eam1104I	CTCTTC	3 cut(s)	686, 741, 1202
EarI	CTCTTC	3 cut(s)	686, 741, 1202
Eco130I	CCWWGG	3 cut(s)	338, 477, 804
Eco147I	AGGCCT	2 cut(s)	10, 1828
Eco32I	GATATC	1 cut(s)	541
Eco57I	CTGAAG	3 cut(s)	72, 1194, 1761
Eco91I	GGTNACC	1 cut(s)	1223
EcoNI	CCTNNNNNAGG	1 cut(s)	691
EcoO109I	RGGNCCY	1 cut(s)	737
EcoO65I	GGTNACC	1 cut(s)	1223
EcoRII	CCWGG	1 cut(s)	1745
EcoRV	GATATC	1 cut(s)	541
EcoT14I	CCWWGG	3 cut(s)	338, 477, 804
EcoT22I	ATGCAT	1 cut(s)	1143
ErhI	CCWWGG	3 cut(s)	338, 477, 804
FaeI	CATG	9 cut(s)	35, 529, 563, 1028, 1078, 1127, 1141, 1424, 1871
FatI	CATG	9 cut(s)	31, 525, 559, 1024, 1074, 1123, 1137, 1420, 1867
FbaI	TGATCA	1 cut(s)	22
FblI	GTMKAC	1 cut(s)	572
Fnu4HI	GCNGC	6 cut(s)	90, 522, 1302, 1634, 1755, 1892
FokI	GGATG	3 cut(s)	1066, 1160, 1399
Fsp4HI	GCNGC	6 cut(s)	90, 522, 1302, 1634, 1755, 1892
FspBI	CTAG	2 cut(s)	186, 1308
GluI	GCNGC	6 cut(s)	90, 522, 1302, 1634, 1755, 1892
HaeIII	GGCC	5 cut(s)	10, 203, 251, 739, 1828
HapII	CCGG	1 cut(s)	634
Hin1II	CATG	9 cut(s)	35, 529, 563, 1028, 1078, 1127, 1141, 1424, 1871
HindIII	AAGCTT	1 cut(s)	438
HinfI	GANTC	9 cut(s)	47, 644, 830, 890, 907, 977, 1276, 1561, 1664
HpaII	CCGG	1 cut(s)	634
HphI	GGTGA	1 cut(s)	1614
Hpy166II	GTNNAC	4 cut(s)	573, 988, 1334, 1467
Hpy188III	TCNNGA	2 cut(s)	365, 1519
Hpy8I	GTNNAC	4 cut(s)	573, 988, 1334, 1467
HpyAV	CCTTC	9 cut(s)	457, 769, 803, 1492, 1600, 1809, 1818, 1870, 1891
HpyCH4III	ACNGT	4 cut(s)	992, 1163, 1764, 1883
HpyF10VI	GCNNNNNNNGC	2 cut(s)	1310, 1787
HpyF3I	CTNAG	5 cut(s)	5, 180, 267, 1537, 1706
Hsp92II	CATG	9 cut(s)	35, 529, 563, 1028, 1078, 1127, 1141, 1424, 1871
Ksp22I	TGATCA	1 cut(s)	22
Kzo9I	GATC	5 cut(s)	22, 270, 426, 760, 1134
LmnI	GCTCC	5 cut(s)	169, 862, 956, 1505, 1846
Lsp1109I	GCAGC	6 cut(s)	101, 533, 1288, 1645, 1766, 1878
LweI	GCATC	3 cut(s)	226, 1377, 1906
MaeI	CTAG	2 cut(s)	186, 1308
MaeIII	GTNAC	2 cut(s)	1223, 1620
MalI	GATC	5 cut(s)	24, 272, 428, 762, 1136
MboI	GATC	5 cut(s)	22, 270, 426, 760, 1134
MfeI	CAATTG	1 cut(s)	608
MhlI	GDGCHC	1 cut(s)	1315
MluCI	AATT	3 cut(s)	13, 41, 608
MlyI	GAGTC	2 cut(s)	41, 1555
Mph1103I	ATGCAT	1 cut(s)	1143
MroXI	GAANNNNTTC	2 cut(s)	883, 1275
MseI	TTAA	5 cut(s)	108, 294, 899, 1043, 1392
MslI	CAYNNNNRTG	5 cut(s)	490, 1128, 1146, 1482, 1573
MspA1I	CMGCKG	1 cut(s)	1301
MspCI	CTTAAG	1 cut(s)	1391
MspI	CCGG	1 cut(s)	634
MspR9I	CCNGG	2 cut(s)	635, 1747
MunI	CAATTG	1 cut(s)	608
Mva1269I	GAATGC	1 cut(s)	652
MvaI	CCWGG	1 cut(s)	1747
MwoI	GCNNNNNNNGC	2 cut(s)	1310, 1787
NciI	CCSGG	1 cut(s)	635
NdeII	GATC	5 cut(s)	22, 270, 426, 760, 1134
NlaIII	CATG	9 cut(s)	35, 529, 563, 1028, 1078, 1127, 1141, 1424, 1871
NlaIV	GGNNCC	4 cut(s)	165, 414, 802, 1848
NmuCI	GTSAC	1 cut(s)	1620
NsiI	ATGCAT	1 cut(s)	1143
NspI	RCATGY	1 cut(s)	563
NspV	TTCGAA	1 cut(s)	115
PceI	AGGCCT	2 cut(s)	10, 1828
PciI	ACATGT	1 cut(s)	559
PctI	GAATGC	1 cut(s)	652
PdmI	GAANNNNTTC	2 cut(s)	883, 1275
PfeI	GAWTC	7 cut(s)	644, 830, 890, 907, 977, 1276, 1664
PkrI	GCNGC	6 cut(s)	91, 523, 1303, 1635, 1756, 1893
PleI	GAGTC	2 cut(s)	41, 1555
PpsI	GAGTC	2 cut(s)	41, 1555
PscI	ACATGT	1 cut(s)	559
Psp6I	CCWGG	1 cut(s)	1745
PspEI	GGTNACC	1 cut(s)	1223
PspGI	CCWGG	1 cut(s)	1745
PspN4I	GGNNCC	4 cut(s)	165, 414, 802, 1848
PspPI	GGNCC	2 cut(s)	202, 737
PstI	CTGCAG	1 cut(s)	1896
PvuII	CAGCTG	1 cut(s)	1301
RsaI	GTAC	3 cut(s)	1795, 1872, 1885
RsaNI	GTAC	3 cut(s)	1794, 1871, 1884
RseI	CAYNNNNRTG	5 cut(s)	490, 1128, 1146, 1482, 1573
SaqAI	TTAA	5 cut(s)	108, 294, 899, 1043, 1392
SatI	GCNGC	6 cut(s)	90, 522, 1302, 1634, 1755, 1892
Sau3AI	GATC	5 cut(s)	22, 270, 426, 760, 1134
Sau96I	GGNCC	2 cut(s)	202, 737
SchI	GAGTC	2 cut(s)	41, 1555
ScrFI	CCNGG	2 cut(s)	635, 1747
SduI	GDGCHC	1 cut(s)	1315
SfaNI	GCATC	3 cut(s)	226, 1377, 1906
SfcI	CTRYAG	3 cut(s)	315, 1101, 1892
SfuI	TTCGAA	1 cut(s)	115
SmiMI	CAYNNNNRTG	5 cut(s)	490, 1128, 1146, 1482, 1573
SmlI	CTYRAG	3 cut(s)	1314, 1391, 1430
SmoI	CTYRAG	3 cut(s)	1314, 1391, 1430
SpeI	ACTAGT	1 cut(s)	1307
Sse9I	AATT	3 cut(s)	13, 41, 608
SseBI	AGGCCT	2 cut(s)	10, 1828
SsiI	CCGC	1 cut(s)	1590
SspMI	CTAG	2 cut(s)	186, 1308
StuI	AGGCCT	2 cut(s)	10, 1828
StyD4I	CCNGG	2 cut(s)	633, 1745
StyI	CCWWGG	3 cut(s)	338, 477, 804
TaaI	ACNGT	4 cut(s)	992, 1163, 1764, 1883
TaqI	TCGA	1 cut(s)	115
TaqII	GACCGA	1 cut(s)	1794
TasI	AATT	3 cut(s)	13, 41, 608
TatI	WGTACW	1 cut(s)	1883
TfiI	GAWTC	7 cut(s)	644, 830, 890, 907, 977, 1276, 1664
Tru1I	TTAA	5 cut(s)	108, 294, 899, 1043, 1392
Tru9I	TTAA	5 cut(s)	108, 294, 899, 1043, 1392
TscAI	CASTG	3 cut(s)	280, 728, 772
TseFI	GTSAC	1 cut(s)	1620
TseI	GCWGC	6 cut(s)	89, 521, 1301, 1633, 1754, 1891
Tsp45I	GTSAC	1 cut(s)	1620
TspDTI	ATGAA	8 cut(s)	48, 474, 542, 636, 759, 1041, 1392, 1409
TspGWI	ACGGA	2 cut(s)	808, 1558
TspRI	CASTG	3 cut(s)	280, 728, 772
Vha464I	CTTAAG	1 cut(s)	1391
XagI	CCTNNNNNAGG	1 cut(s)	691
XceI	RCATGY	1 cut(s)	563
XcmI	CCANNNNNNNNNTGG	1 cut(s)	406
XmiI	GTMKAC	1 cut(s)	572
XmnI	GAANNNNTTC	2 cut(s)	883, 1275
XspI	CTAG	2 cut(s)	186, 1308
Zsp2I	ATGCAT	1 cut(s)	1143

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.