Rorug04G0424600

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0011535
Physical Location & Seq
Forward (+)
58381843 .. 58389605
7763 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0424600.1

Sequence Viewer

Length: 2727 bp
ATGTCAGCCCTCCGGGAACGGTCTCTCCACATCGCCGATTTGCAGGCGTGGATGGACAAGGCGTACTTCGTTAATGACTGCTTCCCAGAGCTTAAGGGAGAGATTGTGAGTGCAAAGTTAATTGTTCAGAAATCGGGTTACTGCTATGGTTTTCTGGAGTTTAGGTCTCACAAGGTAGCAAAGCTTGTTCTGGAGACATACAATGGCAAGCAGATGCCCAAGGCGTGTCAACATTTCCGTCTCAATTGGAGCAGGTCCACGAGGGCATTGCAAACTACAGATGCTTCTCAAGGAGGGAATTACAAGCTTGTAGTTGCTGGGGATCTGCCGAAGGATGTTACTGCTGAAGAACTGCAGCAGCACTTTAGAACCTATTACCCTTCAGTTAAGGATGCTGAAGTTCACACCAGTGAATCCCAGGGGTTGTATGGTGTTATCACATTTGCAAAAGAGCATGAAAGGCATCTGGCCTTAATTCATATGGACATGCAATGTTTTAGGAGGAAAGAAGACGGCAAACCGCATCATTTCTTCTATAAAGACACAGCTGCTGAGTACCAGAGAGCTGCCTACCCAAGTACCCATCCAGAGGATGCACATCCAAATGGTTCAACAATTTACATTCAAAACTTGGAAAAGGATGTTACCGAAGGGTTCTTGCTACGGAAATTTTCCCCCTATGGTGAGGTTTCTAAAGTCACCATAAGGGGATGTGCTCTGATTCAGTTTGTTGATAGATCGAGTGGCGATATAGCATTGCTTCACATGAATGATGACCTCATTCAGCAGAATAGCTTACTTACACTGACCATGAGAAAATTAAATGATGAATCTGTTCCAGTAGCTTCATCTGTTGAAGACTTCAAGGAACTAACAGAGCTTAAGGAATTAACCCATGAAGATGAAAGATCAGTATCTGGGTTCAAAGAGTGTGAAGTCGATTCGTCCTCGTTGGAAATGGCAGACCGCCGTTCCCCGTCTCTCCACATCGCTGATTTGCAGGCCTGGATGGACAAGCCCTACTTGCGGGACGAGTGCTTCCCAAAGTTTCCGGGAGAGGTTATGAGTGCTAGCATTTTTGTGGAGAAATGTGGTTATCGCTATGGTTTTCTGGAGTTTAGGTCTCCTGAGGTAGCAGAGCTTGTACGCAAGACGTACAATGGCAAGAGGATGCCCAAGGCGTGTCAATATTTCCGTCTCAATAGGAACAGGTCCAAGAGACCACCGGGAACTATGGCTGCACCTGAAGAAGGGAATTACAAGCTTGTAGTTGATGGGGATTTGCCGAAGGATGTTACTGCTGAAGAACTGCAGCAGCACTTTAGAACCTATTACCCTTCAGTTAAGGATGCTGAAGTTCACACCAGTGAATCCCAGGGGTTGTATGGTGTTATTACCTTTGCAAAGGAGCATGAAAGGGATCTAGCATCAATTCATATGGCCAGTCAATGTTTTAGGAGGACAGAAGACGGCAAACCGCATCATTTCTTCTATAAAGATACAGCTGCTGAGTACCAGAGAGCTGCCTACCCAAGTACCCATCCAGAGGATGCACATCCAAATGGTTCAAAAATTTACATTCAAAACTTGGAAATGGATGTCACTAAAGGGTTCTTGCTACGGAAATTTTCTCCCTATGGTGAGGTTTCTAAAATCACCATAAGGGGATGTGCTCTGATTCAGTTTGTTGATAGATCGAGTGCTGATAAAGCATTGCTTCACATGAATGATGACCTCATTCTGCAGAATAGCTTACTTCCACTGACTGTGAGAAAATCAAATGATGAATCTGTTCCAGTAGCTTCATCTGTTGAAGATCTTAAGGAATTAACCCATGAAGATGAAAGATCAGTATCTGGGTTCAGAGCGTGTGAGGAGGAGTCCACAGATGAAAAAATAACAGAGGAGAGGGAGCAAGAAAATGATGAATCTGTTCCAGTAGCTTCAGCTGTAGAAGATCTTGAGGAACTAACACATGGAGAATCACCGAAAGACCTTAAGGAATTAACCCATGACGATGAGGGATCAGTATCTGGGTTTAGAACGTGTGAGGAGGAGTCCACAGATGAAATAACAGACGAGGGGAGGCAAGAAAATGATGAATCTGTTCCAGTAGCTTCAGCTGTAGAAGATCTTGAGGAACTAACACATGGAGAATCACCGAAAGACCTTAAGGAATTAACCCATGACGATGAAGGATCAGTATCTGGGTTTAGAACGTGTGAGGAGGAGTCCACAGATGAAATAACAGACGAGGGGAGGCAAGAAAATGATGAATCTGTTCCAGTAGCTTCATCTGTAGAAGACCTTGAAGAACAAAAACATGGAGAATCACCGAAAGACCGTAAGGAATTAACCCATGAAGATGAAGGATTAGTATCTGGGTTCAGAACGTGTGAGGAGGAGCCCACAGATGAAAAAATAACAGAGGAGGGGAAGCAAGAAGAGGAGGTGAAAAAAGTCAGAAGGGCAAAGACGGAGGTTAGTGAAGGACAGAGACGGAGGAGGAAGGCGAAGAAGGCAAGGCAGAAGGAGAAAAAGAAGCTTCTTAAGCAAACCGATGAACGGTTGGAGGCATCAGAAGGGCAGCAAACCAATGAAGGGTTTGAAGCACCAGAAGGGCAGCAAACCAATGAAGGCTCGCAAGCATCAGAAGGGCATCAATTGGTCATCTCTTGGCTGTGGGAGTTGTATGCGAGCCTGAAGCACTACTTGTTCGGCTGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

908

Amino Acids

103.39

Weight (kDa)

5.05

Isoelectric Point (pI)

53.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RRM_1 PF00076 525 - 583 7.1e-07 RNA recognition motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 243
AciI CCGC 4 cut(s) 521, 967, 1027, 1478
AclWI GGATC 4 cut(s) 330, 1428, 2032, 2206
AcoI YGGCCR 1 cut(s) 1440
AcsI RAATTY 3 cut(s) 668, 1572, 1625
AfaI GTAC 7 cut(s) 65, 557, 580, 1146, 1157, 1514, 1537
AfiI CCNNNNNNNGG 6 cut(s) 589, 1026, 1250, 1546, 2565, 2601
AflII CTTAAG 6 cut(s) 92, 881, 1820, 1997, 2171, 2549
AflIII ACRYGT 3 cut(s) 2045, 2219, 2393
AjnI CCWGG 3 cut(s) 417, 1004, 1374
AleI CACNNNNGTG 2 cut(s) 408, 1365
AloI GAACNNNNNNTCC 2 cut(s) 9, 41
Alw21I GWGCWC 2 cut(s) 718, 1675
Alw26I GTCTC 9 cut(s) 27, 171, 188, 245, 984, 1128, 1202, 1213, 2491
AlwI GGATC 4 cut(s) 330, 1428, 2032, 2206
AlwNI CAGNNNCTG 6 cut(s) 551, 917, 1508, 1856, 2033, 2207
AoxI GGCC 3 cut(s) 468, 1002, 1440
ApoI RAATTY 3 cut(s) 668, 1572, 1625
Asp700I GAANNNNTTC 5 cut(s) 834, 1791, 1932, 2106, 2280
AspS9I GGNCC 2 cut(s) 255, 1212
AsuC2I CCSGG 3 cut(s) 14, 1053, 1227
AsuHPI GGTGA 8 cut(s) 691, 695, 1648, 1652, 1977, 2151, 2325, 2464
AsuNHI GCTAGC 1 cut(s) 1070
AvaII GGWCC 2 cut(s) 255, 1212
AxyI CCTNAGG 1 cut(s) 1128
BaeI ACNNNNGTAYC 4 cut(s) 562, 595, 1519, 1552
BalI TGGCCA 1 cut(s) 1442
BanII GRGCYC 1 cut(s) 2409
BauI CACGAG 1 cut(s) 259
BbsI GAAGAC 4 cut(s) 516, 864, 1473, 2310
Bbv12I GWGCWC 2 cut(s) 718, 1675
BccI CCATC 5 cut(s) 46, 591, 1003, 1268, 1548
BceAI ACGGC 3 cut(s) 529, 954, 1486
BcgI CGANNNNNNTGC 2 cut(s) 250, 284
BciT130I CCWGG 3 cut(s) 419, 1006, 1376
BcnI CCSGG 3 cut(s) 14, 1053, 1227
BcoDI GTCTC 9 cut(s) 27, 171, 188, 245, 984, 1128, 1202, 1213, 2491
BfaI CTAG 2 cut(s) 1071, 1424
BfmI CTRYAG 7 cut(s) 276, 353, 1310, 1742, 1950, 2124, 2298
BfrI CTTAAG 6 cut(s) 92, 881, 1820, 1997, 2171, 2549
BfuAI ACCTGC 1 cut(s) 243
BglII AGATCT 3 cut(s) 1816, 1957, 2131
Bme1390I CCNGG 6 cut(s) 14, 419, 1006, 1053, 1227, 1376
Bme18I GGWCC 2 cut(s) 255, 1212
BmgT120I GGNCC 2 cut(s) 255, 1212
BmiI GGNNCC 1 cut(s) 2406
BmrFI CCNGG 6 cut(s) 14, 419, 1006, 1053, 1227, 1376
BmtI GCTAGC 1 cut(s) 1074
BpiI GAAGAC 4 cut(s) 516, 864, 1473, 2310
BpmI CTGGAG 3 cut(s) 176, 212, 1133
BpuEI CTTGAG 3 cut(s) 273, 1982, 2156
BpuMI CCSGG 3 cut(s) 14, 1053, 1227
BsaBI GATNNNNATC 7 cut(s) 597, 913, 1554, 1852, 2029, 2203, 2377
BsaI GGTCTC 4 cut(s) 27, 171, 1128, 1213
BsaJI CCNNGG 6 cut(s) 219, 417, 418, 1176, 1374, 1375
BsaXI ACNNNNNCTCC 8 cut(s) 9, 39, 90, 120, 149, 179, 1106, 1136
Bsc4I CCNNNNNNNGG 6 cut(s) 589, 1026, 1250, 1546, 2565, 2601
Bse1I ACTGG 8 cut(s) 408, 839, 1365, 1443, 1796, 1937, 2111, 2285
Bse21I CCTNAGG 1 cut(s) 1128
Bse3DI GCAATG 4 cut(s) 266, 497, 755, 1712
Bse8I GATNNNNATC 7 cut(s) 597, 913, 1554, 1852, 2029, 2203, 2377
BseBI CCWGG 3 cut(s) 419, 1006, 1376
BseDI CCNNGG 6 cut(s) 219, 417, 418, 1176, 1374, 1375
BseJI GATNNNNATC 7 cut(s) 597, 913, 1554, 1852, 2029, 2203, 2377
BseLI CCNNNNNNNGG 6 cut(s) 589, 1026, 1250, 1546, 2565, 2601
BseMI GCAATG 4 cut(s) 266, 497, 755, 1712
BseMII CTCAG 3 cut(s) 543, 1119, 1500
BseNI ACTGG 8 cut(s) 408, 839, 1365, 1443, 1796, 1937, 2111, 2285
BseYI CCCAGC 1 cut(s) 317
BsgI GTGCAG 1 cut(s) 1224
BshFI GGCC 3 cut(s) 470, 1004, 1442
BsiHKAI GWGCWC 2 cut(s) 718, 1675
BsiSI CCGG 3 cut(s) 13, 1052, 1226
BslFI GGGAC 1 cut(s) 1043
BslI CCNNNNNNNGG 6 cut(s) 589, 1026, 1250, 1546, 2565, 2601
BsmAI GTCTC 9 cut(s) 27, 171, 188, 245, 984, 1128, 1202, 1213, 2491
BsmBI CGTCTC 4 cut(s) 245, 984, 1202, 2491
BsmFI GGGAC 1 cut(s) 1043
BsnI GGCC 3 cut(s) 470, 1004, 1442
Bso31I GGTCTC 4 cut(s) 27, 171, 1128, 1213
Bsp1286I GDGCHC 3 cut(s) 718, 1675, 2409
BspACI CCGC 4 cut(s) 521, 967, 1027, 1478
BspANI GGCC 3 cut(s) 470, 1004, 1442
BspCNI CTCAG 3 cut(s) 544, 1120, 1501
BspLI GGNNCC 1 cut(s) 2406
BspMAI CTGCAG 3 cut(s) 357, 1314, 1746
BspMI ACCTGC 1 cut(s) 243
BspOI GCTAGC 1 cut(s) 1074
BspPI GGATC 4 cut(s) 330, 1428, 2032, 2206
BspTI CTTAAG 6 cut(s) 92, 881, 1820, 1997, 2171, 2549
BspTNI GGTCTC 4 cut(s) 27, 171, 1128, 1213
BsrDI GCAATG 4 cut(s) 266, 497, 755, 1712
BsrI ACTGG 8 cut(s) 408, 839, 1365, 1443, 1796, 1937, 2111, 2285
BssECI CCNNGG 6 cut(s) 219, 417, 418, 1176, 1374, 1375
BssSI CACGAG 1 cut(s) 259
BssT1I CCWWGG 2 cut(s) 219, 1176
Bst2BI CACGAG 1 cut(s) 259
Bst2UI CCWGG 3 cut(s) 419, 1006, 1376
Bst4CI ACNGT 4 cut(s) 21, 1768, 2345, 2568
Bst6I CTCTTC 1 cut(s) 2439
BstAFI CTTAAG 6 cut(s) 92, 881, 1820, 1997, 2171, 2549
BstC8I GCNNGC 7 cut(s) 45, 209, 1002, 1072, 2642, 2646, 2698
BstDEI CTNAG 3 cut(s) 552, 1128, 1509
BstENI CCTNNNNNAGG 1 cut(s) 1248
BstMAI GTCTC 9 cut(s) 27, 171, 188, 245, 984, 1128, 1202, 1213, 2491
BstMWI GCNNNNNNNGC 5 cut(s) 460, 1024, 1709, 2519, 2551
BstNI CCWGG 3 cut(s) 419, 1006, 1376
BstNSI RCATGY 1 cut(s) 490
BstSCI CCNGG 6 cut(s) 12, 417, 1004, 1051, 1225, 1374
BstSFI CTRYAG 7 cut(s) 276, 353, 1310, 1742, 1950, 2124, 2298
BstV2I GAAGAC 4 cut(s) 516, 864, 1473, 2310
BstX2I RGATCY 5 cut(s) 322, 1420, 1816, 1957, 2131
BstYI RGATCY 5 cut(s) 322, 1420, 1816, 1957, 2131
Bsu36I CCTNAGG 1 cut(s) 1128
BsuRI GGCC 3 cut(s) 470, 1004, 1442
BtgZI GCGATG 2 cut(s) 16, 973
BtsIMutI CAGTG 4 cut(s) 415, 803, 1372, 1760
BveI ACCTGC 1 cut(s) 243
Cac8I GCNNGC 7 cut(s) 45, 209, 1002, 1072, 2642, 2646, 2698
CaiI CAGNNNCTG 6 cut(s) 551, 917, 1508, 1856, 2033, 2207
Cfr13I GGNCC 2 cut(s) 255, 1212
Csp6I GTAC 7 cut(s) 64, 556, 579, 1145, 1156, 1513, 1536
CviQI GTAC 7 cut(s) 64, 556, 579, 1145, 1156, 1513, 1536
DdeI CTNAG 3 cut(s) 552, 1128, 1509
EaeI YGGCCR 1 cut(s) 1440
Eam1104I CTCTTC 1 cut(s) 2439
EarI CTCTTC 1 cut(s) 2439
Eco130I CCWWGG 2 cut(s) 219, 1176
Eco147I AGGCCT 1 cut(s) 1004
Eco24I GRGCYC 1 cut(s) 2409
Eco31I GGTCTC 4 cut(s) 27, 171, 1128, 1213
Eco47I GGWCC 2 cut(s) 255, 1212
Eco81I CCTNAGG 1 cut(s) 1128
EcoNI CCTNNNNNAGG 1 cut(s) 1248
EcoRII CCWGG 3 cut(s) 417, 1004, 1374
EcoT14I CCWWGG 2 cut(s) 219, 1176
EcoT38I GRGCYC 1 cut(s) 2409
ErhI CCWWGG 2 cut(s) 219, 1176
Esp3I CGTCTC 4 cut(s) 245, 984, 1202, 2491
FalI AAGNNNNNCTT 7 cut(s) 50, 82, 1007, 1039, 1701, 1733, 2696
FaqI GGGAC 1 cut(s) 1043
FauI CCCGC 1 cut(s) 1020
FauNDI CATATG 2 cut(s) 480, 1437
FriOI GRGCYC 1 cut(s) 2409
FspBI CTAG 2 cut(s) 1071, 1424
GsaI CCCAGC 1 cut(s) 321
GsuI CTGGAG 3 cut(s) 176, 212, 1133
HaeIII GGCC 3 cut(s) 470, 1004, 1442
HapII CCGG 3 cut(s) 13, 1052, 1226
HincII GTYRAC 1 cut(s) 230
HindII GTYRAC 1 cut(s) 230
HindIII AAGCTT 4 cut(s) 182, 305, 1262, 2543
HpaII CCGG 3 cut(s) 13, 1052, 1226
HphI GGTGA 8 cut(s) 691, 695, 1648, 1652, 1977, 2151, 2325, 2464
Hpy166II GTNNAC 7 cut(s) 230, 258, 403, 1360, 1884, 2061, 2235
Hpy188I TCNGA 8 cut(s) 129, 720, 1677, 1865, 2390, 2465, 2581, 2653
Hpy188III TCNNGA 8 cut(s) 155, 191, 587, 1112, 1127, 1544, 1961, 2135
Hpy8I GTNNAC 7 cut(s) 230, 258, 403, 1360, 1884, 2061, 2235
HpyCH4III ACNGT 4 cut(s) 21, 1768, 2345, 2568
HpyCH4IV ACGT 4 cut(s) 1154, 2045, 2219, 2393
HpyF10VI GCNNNNNNNGC 5 cut(s) 460, 1024, 1709, 2519, 2551
HpyF3I CTNAG 3 cut(s) 552, 1128, 1509
HpySE526I ACGT 4 cut(s) 1154, 2045, 2219, 2393
LmnI GCTCC 4 cut(s) 249, 1408, 1912, 2404
MaeI CTAG 2 cut(s) 1071, 1424
MaeII ACGT 4 cut(s) 1154, 2045, 2219, 2393
MaeIII GTNAC 6 cut(s) 137, 337, 643, 697, 1294, 1600
MfeI CAATTG 2 cut(s) 244, 2663
MflI RGATCY 5 cut(s) 322, 1420, 1816, 1957, 2131
MhlI GDGCHC 3 cut(s) 718, 1675, 2409
MlsI TGGCCA 1 cut(s) 1442
MluNI TGGCCA 1 cut(s) 1442
MlyI GAGTC 3 cut(s) 1889, 2066, 2240
MmeI TCCRAC 2 cut(s) 933, 2550
Mox20I TGGCCA 1 cut(s) 1442
MroXI GAANNNNTTC 5 cut(s) 834, 1791, 1932, 2106, 2280
MscI TGGCCA 1 cut(s) 1442
Msp20I TGGCCA 1 cut(s) 1442
MspA1I CMGCKG 4 cut(s) 548, 1505, 1949, 2123
MspCI CTTAAG 6 cut(s) 92, 881, 1820, 1997, 2171, 2549
MspI CCGG 3 cut(s) 13, 1052, 1226
MspR9I CCNGG 6 cut(s) 14, 419, 1006, 1053, 1227, 1376
MunI CAATTG 2 cut(s) 244, 2663
MvaI CCWGG 3 cut(s) 419, 1006, 1376
MwoI GCNNNNNNNGC 5 cut(s) 460, 1024, 1709, 2519, 2551
NciI CCSGG 3 cut(s) 14, 1053, 1227
NdeI CATATG 2 cut(s) 480, 1437
NheI GCTAGC 1 cut(s) 1070
NlaIV GGNNCC 1 cut(s) 2406
NmuCI GTSAC 2 cut(s) 697, 1600
NspI RCATGY 1 cut(s) 490
OliI CACNNNNGTG 2 cut(s) 408, 1365
PasI CCCWGGG 2 cut(s) 418, 1375
PceI AGGCCT 1 cut(s) 1004
PdmI GAANNNNTTC 5 cut(s) 834, 1791, 1932, 2106, 2280
PfoI TCCNGGA 2 cut(s) 12, 1051
PleI GAGTC 3 cut(s) 1888, 2065, 2239
PpsI GAGTC 3 cut(s) 1888, 2065, 2239
Psp6I CCWGG 3 cut(s) 417, 1004, 1374
PspFI CCCAGC 1 cut(s) 317
PspGI CCWGG 3 cut(s) 417, 1004, 1374
PspN4I GGNNCC 1 cut(s) 2406
PspPI GGNCC 2 cut(s) 255, 1212
PstI CTGCAG 3 cut(s) 357, 1314, 1746
PstNI CAGNNNCTG 6 cut(s) 551, 917, 1508, 1856, 2033, 2207
PsuI RGATCY 5 cut(s) 322, 1420, 1816, 1957, 2131
PvuII CAGCTG 4 cut(s) 548, 1505, 1949, 2123
RsaI GTAC 7 cut(s) 65, 557, 580, 1146, 1157, 1514, 1537
RsaNI GTAC 7 cut(s) 64, 556, 579, 1145, 1156, 1513, 1536
Sau96I GGNCC 2 cut(s) 255, 1212
SchI GAGTC 3 cut(s) 1889, 2066, 2240
ScrFI CCNGG 6 cut(s) 14, 419, 1006, 1053, 1227, 1376
SduI GDGCHC 3 cut(s) 718, 1675, 2409
SfcI CTRYAG 7 cut(s) 276, 353, 1310, 1742, 1950, 2124, 2298
SinI GGWCC 2 cut(s) 255, 1212
SmlI CTYRAG 9 cut(s) 92, 288, 881, 1820, 1961, 1997, 2135, 2171, 2549
SmoI CTYRAG 9 cut(s) 92, 288, 881, 1820, 1961, 1997, 2135, 2171, 2549
SseBI AGGCCT 1 cut(s) 1004
SsiI CCGC 4 cut(s) 521, 967, 1027, 1478
SspI AATATT 1 cut(s) 1190
SspMI CTAG 2 cut(s) 1071, 1424
StuI AGGCCT 1 cut(s) 1004
StyD4I CCNGG 6 cut(s) 12, 417, 1004, 1051, 1225, 1374
StyI CCWWGG 2 cut(s) 219, 1176
TaaI ACNGT 4 cut(s) 21, 1768, 2345, 2568
TaiI ACGT 4 cut(s) 1157, 2048, 2222, 2396
TaqI TCGA 3 cut(s) 740, 939, 1697
TscAI CASTG 4 cut(s) 415, 810, 1372, 1767
TseFI GTSAC 2 cut(s) 697, 1600
Tsp45I GTSAC 2 cut(s) 697, 1600
TspGWI ACGGA 6 cut(s) 227, 679, 1184, 1636, 2492, 2515
TspRI CASTG 4 cut(s) 415, 810, 1372, 1767
Vha464I CTTAAG 6 cut(s) 92, 881, 1820, 1997, 2171, 2549
VpaK11BI GGWCC 2 cut(s) 255, 1212
XagI CCTNNNNNAGG 1 cut(s) 1248
XapI RAATTY 3 cut(s) 668, 1572, 1625
XceI RCATGY 1 cut(s) 490
XcmI CCANNNNNNNNNTGG 2 cut(s) 425, 1382
XmnI GAANNNNTTC 5 cut(s) 834, 1791, 1932, 2106, 2280
XspI CTAG 2 cut(s) 1071, 1424
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.