Rorug04G0430200
ERF Family

Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0003562
Physical Location & Seq
Forward (+)
58895428 .. 58897011
1584 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0430200.1

Sequence Viewer

Length: 981 bp
ATGGCACAGATCGAACAGATTAGCGAGTACCAAGCCTCCATGATTGTTGATGATGACGAAAAAAGCCACGATTCCGGGTCGGACTGCTCCAAATCGAGCTCCGCCTCGGTCGTTTCGGATCGGTTCGAGGTTTTCACTAGGATGAGATTGCTTAGGTTGGAAGAAGATAGTATAGAGCATGAGTTTACCAAGAAGATGTTCGCCGTGGGTATGGAGTCGAACGGAGTAGACGCCAAGGTTGTGGCGGTTCACAAGAATCTGCATTCTGGGTGGACCAGGCGAGCCAGGTGCGAAACGTTTCTGATCTTCCAGAAGGCGGTGGCTCAGAAATGCGGCGGAAACGCCAATATCGGATACGGCTGGTACGGCGGGTCAAGGGATGAGATTTGTGATATCCTGTTGCATGGGTTTAGTGGGTGCAGAGCGGCCAGGTCTCATGGGGTTGGAATTCAATTGTTTGCTAATAACTATTCTTTTGATGGTGCAATATCATCAGACGCTGATGAGAATGGGCTGAGGCACATGTTGCTGTGCAGAGTCATATTGGGGAAGAGAGAATTGGTGCCCGCTGGTTCGAAGCAAGTTAATCCAAGTTCCAAGGAGTTTGATTCTGGTGTGGACAGTCTTGTGAATCCGAAAAGATTTATCATATGGAGCTCTTTCATGAACTCTCATATTATGCCTCTTTTTGTTGTGAGCTTCAAGGCTCCTGCTAACTTGAAGGATATTCCAAGGAGCATTCAGGGGAGTGCTGCTGCTGTAAGACCACCACCCAAGTCGGCTTGGGTGAATCTCACTGCTATGATGAACGCCCTCGCTAAGGTCTTGCCTCCTCCTAGAATGGTTCTGCTTTCCAAATCCGCTGACGATTTCAGGGCGAAGAAGATTACTAGACCTCAGCTAATTAGAAGAGTGAGAACTGTAGCAGGTGACAAATTGCTGGTTTCGGTGGTCAAAAACTTTAACAAGGAGCGGGAGTGA

Protein Analysis

326

Amino Acids

36.09

Weight (kDa)

9.35

Isoelectric Point (pI)

40.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RST PF12174 258 - 321 5.8e-18 RCD1-SRO-TAF4 (RST) plant domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 917
Acc36I ACCTGC 1 cut(s) 917
AccB1I GGYRCC 1 cut(s) 562
AccBSI CCGCTC 2 cut(s) 425, 973
AccI GTMKAC 1 cut(s) 228
AclI AACGTT 1 cut(s) 296
AclWI GGATC 1 cut(s) 126
AcoI YGGCCR 1 cut(s) 426
AcsI RAATTY 1 cut(s) 447
AcyI GRCGYC 1 cut(s) 231
AfaI GTAC 2 cut(s) 29, 365
AfiI CCNNNNNNNGG 2 cut(s) 316, 820
AflIII ACRYGT 1 cut(s) 522
AgsI TTSAA 3 cut(s) 452, 703, 721
AjnI CCWGG 3 cut(s) 275, 284, 428
AjuI GAANNNNNNNTTGG 4 cut(s) 182, 214, 542, 574
AluBI AGCT 4 cut(s) 99, 657, 699, 901
AluI AGCT 4 cut(s) 99, 657, 699, 901
Alw21I GWGCWC 2 cut(s) 101, 659
Alw26I GTCTC 1 cut(s) 438
AlwI GGATC 1 cut(s) 126
AlwNI CAGNNNCTG 1 cut(s) 500
AoxI GGCC 1 cut(s) 426
ApeKI GCWGC 2 cut(s) 752, 755
ApoI RAATTY 1 cut(s) 447
Asp700I GAANNNNTTC 2 cut(s) 197, 297
AspS9I GGNCC 1 cut(s) 273
AsuC2I CCSGG 1 cut(s) 76
AsuHPI GGTGA 2 cut(s) 799, 941
AsuII TTCGAA 1 cut(s) 575
AvaII GGWCC 1 cut(s) 273
BaeGI GKGCMC 1 cut(s) 567
BanI GGYRCC 1 cut(s) 562
BanII GRGCYC 2 cut(s) 101, 659
Bbv12I GWGCWC 2 cut(s) 101, 659
BbvCI CCTCAGC 2 cut(s) 515, 897
BbvI GCAGC 2 cut(s) 739, 742
BccI CCATC 1 cut(s) 473
BceAI ACGGC 3 cut(s) 188, 373, 382
BcgI CGANNNNNNTGC 2 cut(s) 270, 304
BciT130I CCWGG 3 cut(s) 277, 286, 430
BciVI GTATCC 1 cut(s) 347
BcnI CCSGG 1 cut(s) 76
BcoDI GTCTC 1 cut(s) 438
BfaI CTAG 3 cut(s) 138, 837, 891
BfmI CTRYAG 1 cut(s) 921
BfuAI ACCTGC 1 cut(s) 917
BfuI GTATCC 1 cut(s) 347
BisI GCNGC 4 cut(s) 334, 426, 753, 756
BlsI GCNGC 4 cut(s) 335, 427, 754, 757
Bme1390I CCNGG 4 cut(s) 76, 277, 286, 430
Bme18I GGWCC 1 cut(s) 273
BmgT120I GGNCC 1 cut(s) 273
BmiI GGNNCC 2 cut(s) 564, 708
BmrFI CCNGG 4 cut(s) 76, 277, 286, 430
Bpu10I CCTNAGC 4 cut(s) 152, 515, 819, 897
Bpu14I TTCGAA 1 cut(s) 575
BpuMI CCSGG 1 cut(s) 76
BsaHI GRCGYC 1 cut(s) 231
BsaI GGTCTC 1 cut(s) 438
BsaJI CCNNGG 5 cut(s) 105, 204, 234, 597, 731
BsaXI ACNNNNNCTCC 3 cut(s) 20, 50, 962
Bsc4I CCNNNNNNNGG 2 cut(s) 316, 820
BseBI CCWGG 3 cut(s) 277, 286, 430
BseDI CCNNGG 5 cut(s) 105, 204, 234, 597, 731
BseGI GGATG 2 cut(s) 147, 385
BseLI CCNNNNNNNGG 2 cut(s) 316, 820
BseMII CTCAG 3 cut(s) 338, 506, 911
BseRI GAGGAG 1 cut(s) 822
BseSI GKGCMC 1 cut(s) 567
BseXI GCAGC 2 cut(s) 739, 742
BsgI GTGCAG 2 cut(s) 439, 553
Bsh1285I CGRYCG 1 cut(s) 111
BshFI GGCC 1 cut(s) 428
BshNI GGYRCC 1 cut(s) 562
BsiEI CGRYCG 1 cut(s) 111
BsiHKAI GWGCWC 2 cut(s) 101, 659
BsiSI CCGG 1 cut(s) 75
BslI CCNNNNNNNGG 2 cut(s) 316, 820
BsmAI GTCTC 1 cut(s) 438
BsmI GAATGC 2 cut(s) 262, 738
BsnI GGCC 1 cut(s) 428
Bso31I GGTCTC 1 cut(s) 438
Bsp119I TTCGAA 1 cut(s) 575
Bsp1286I GDGCHC 3 cut(s) 101, 567, 659
Bsp143I GATC 3 cut(s) 9, 118, 303
BspANI GGCC 1 cut(s) 428
BspCNI CTCAG 3 cut(s) 337, 507, 910
BspHI TCATGA 1 cut(s) 663
BspLI GGNNCC 2 cut(s) 564, 708
BspMI ACCTGC 1 cut(s) 917
BspPI GGATC 1 cut(s) 126
BspT104I TTCGAA 1 cut(s) 575
BspT107I GGYRCC 1 cut(s) 562
BspTNI GGTCTC 1 cut(s) 438
BsrBI CCGCTC 2 cut(s) 425, 973
BssECI CCNNGG 5 cut(s) 105, 204, 234, 597, 731
BssMI GATC 3 cut(s) 9, 118, 303
BssNI GRCGYC 1 cut(s) 231
BssT1I CCWWGG 3 cut(s) 234, 597, 731
Bst2UI CCWGG 3 cut(s) 277, 286, 430
Bst4CI ACNGT 2 cut(s) 623, 922
Bst6I CTCTTC 2 cut(s) 545, 904
BstACI GRCGYC 1 cut(s) 231
BstAPI GCANNNNNTGC 1 cut(s) 526
BstBI TTCGAA 1 cut(s) 575
BstC8I GCNNGC 2 cut(s) 282, 567
BstDEI CTNAG 5 cut(s) 152, 324, 515, 819, 897
BstDSI CCRYGG 1 cut(s) 204
BstENI CCTNNNNNAGG 1 cut(s) 818
BstF5I GGATG 2 cut(s) 147, 385
BstKTI GATC 3 cut(s) 12, 121, 306
BstMAI GTCTC 1 cut(s) 438
BstMBI GATC 3 cut(s) 9, 118, 303
BstMCI CGRYCG 1 cut(s) 111
BstMWI GCNNNNNNNGC 2 cut(s) 366, 526
BstNI CCWGG 3 cut(s) 277, 286, 430
BstNSI RCATGY 1 cut(s) 526
BstSCI CCNGG 4 cut(s) 74, 275, 284, 428
BstSFI CTRYAG 1 cut(s) 921
BstSLI GKGCMC 1 cut(s) 567
BstV1I GCAGC 2 cut(s) 739, 742
BstXI CCANNNNNNTGG 1 cut(s) 241
BsuI GTATCC 1 cut(s) 347
BsuRI GGCC 1 cut(s) 428
BtgI CCRYGG 1 cut(s) 204
BtsCI GGATG 2 cut(s) 147, 385
BtsI GCAGTG 1 cut(s) 795
BtsIMutI CAGTG 1 cut(s) 795
BveI ACCTGC 1 cut(s) 917
Cac8I GCNNGC 2 cut(s) 282, 567
CaiI CAGNNNCTG 1 cut(s) 500
CciI TCATGA 1 cut(s) 663
Cfr13I GGNCC 1 cut(s) 273
CseI GACGC 2 cut(s) 239, 506
Csp6I GTAC 2 cut(s) 28, 364
CviAII CATG 6 cut(s) 40, 179, 404, 437, 523, 664
CviQI GTAC 2 cut(s) 28, 364
DdeI CTNAG 5 cut(s) 152, 324, 515, 819, 897
DpnI GATC 3 cut(s) 11, 120, 305
DpnII GATC 3 cut(s) 9, 118, 303
EaeI YGGCCR 1 cut(s) 426
Eam1104I CTCTTC 2 cut(s) 545, 904
EarI CTCTTC 2 cut(s) 545, 904
EciI GGCGGA 2 cut(s) 91, 351
Ecl136II GAGCTC 2 cut(s) 99, 657
Eco130I CCWWGG 3 cut(s) 234, 597, 731
Eco24I GRGCYC 2 cut(s) 101, 659
Eco31I GGTCTC 1 cut(s) 438
Eco32I GATATC 1 cut(s) 394
Eco47I GGWCC 1 cut(s) 273
Eco53kI GAGCTC 2 cut(s) 99, 657
EcoICRI GAGCTC 2 cut(s) 99, 657
EcoNI CCTNNNNNAGG 1 cut(s) 818
EcoRI GAATTC 1 cut(s) 447
EcoRII CCWGG 3 cut(s) 275, 284, 428
EcoRV GATATC 1 cut(s) 394
EcoT14I CCWWGG 3 cut(s) 234, 597, 731
EcoT38I GRGCYC 2 cut(s) 101, 659
ErhI CCWWGG 3 cut(s) 234, 597, 731
FaeI CATG 6 cut(s) 43, 182, 407, 440, 526, 667
FatI CATG 6 cut(s) 39, 178, 403, 436, 522, 663
FauI CCCGC 3 cut(s) 362, 574, 966
FauNDI CATATG 1 cut(s) 650
FblI GTMKAC 1 cut(s) 228
Fnu4HI GCNGC 4 cut(s) 334, 426, 753, 756
FokI GGATG 2 cut(s) 154, 392
FriOI GRGCYC 2 cut(s) 101, 659
Fsp4HI GCNGC 4 cut(s) 334, 426, 753, 756
FspBI CTAG 3 cut(s) 138, 837, 891
GluI GCNGC 4 cut(s) 334, 426, 753, 756
HaeIII GGCC 1 cut(s) 428
HapII CCGG 1 cut(s) 75
HgaI GACGC 2 cut(s) 239, 506
Hin1I GRCGYC 1 cut(s) 231
Hin1II CATG 6 cut(s) 43, 182, 407, 440, 526, 667
HinfI GANTC 7 cut(s) 71, 215, 256, 537, 608, 631, 790
HpaII CCGG 1 cut(s) 75
HphI GGTGA 2 cut(s) 799, 941
Hpy166II GTNNAC 5 cut(s) 186, 229, 250, 273, 619
Hpy188I TCNGA 7 cut(s) 82, 118, 303, 327, 353, 496, 636
Hpy188III TCNNGA 2 cut(s) 310, 664
Hpy8I GTNNAC 5 cut(s) 186, 229, 250, 273, 619
HpyAV CCTTC 2 cut(s) 307, 715
HpyCH4III ACNGT 2 cut(s) 623, 922
HpyCH4IV ACGT 1 cut(s) 296
HpyCH4V TGCA 5 cut(s) 262, 403, 420, 485, 534
HpyF10VI GCNNNNNNNGC 2 cut(s) 366, 526
HpyF3I CTNAG 5 cut(s) 152, 324, 515, 819, 897
HpySE526I ACGT 1 cut(s) 296
Hsp92I GRCGYC 1 cut(s) 231
Hsp92II CATG 6 cut(s) 43, 182, 407, 440, 526, 667
Kzo9I GATC 3 cut(s) 9, 118, 303
LmnI GCTCC 6 cut(s) 92, 104, 654, 712, 735, 970
Lsp1109I GCAGC 2 cut(s) 739, 742
MaeI CTAG 3 cut(s) 138, 837, 891
MaeII ACGT 1 cut(s) 296
MaeIII GTNAC 1 cut(s) 929
MalI GATC 3 cut(s) 11, 120, 305
MbiI CCGCTC 2 cut(s) 425, 973
MboI GATC 3 cut(s) 9, 118, 303
MboII GAAGA 8 cut(s) 173, 176, 205, 298, 562, 892, 895, 921
MfeI CAATTG 1 cut(s) 452
MhlI GDGCHC 3 cut(s) 101, 567, 659
MluCI AATT 5 cut(s) 447, 452, 557, 903, 935
MlyI GAGTC 2 cut(s) 224, 546
MmeI TCCRAC 3 cut(s) 60, 138, 424
MnlI CCTC 9 cut(s) 46, 115, 121, 510, 693, 824, 840, 843, 906
MroXI GAANNNNTTC 2 cut(s) 197, 297
MseI TTAA 2 cut(s) 585, 963
MslI CAYNNNNRTG 2 cut(s) 140, 800
MspA1I CMGCKG 2 cut(s) 569, 863
MspI CCGG 1 cut(s) 75
MspR9I CCNGG 4 cut(s) 76, 277, 286, 430
MunI CAATTG 1 cut(s) 452
Mva1269I GAATGC 2 cut(s) 262, 738
MvaI CCWGG 3 cut(s) 277, 286, 430
MwoI GCNNNNNNNGC 2 cut(s) 366, 526
NciI CCSGG 1 cut(s) 76
NdeI CATATG 1 cut(s) 650
NdeII GATC 3 cut(s) 9, 118, 303
NlaIII CATG 6 cut(s) 43, 182, 407, 440, 526, 667
NlaIV GGNNCC 2 cut(s) 564, 708
NmuCI GTSAC 1 cut(s) 929
NspI RCATGY 1 cut(s) 526
NspV TTCGAA 1 cut(s) 575
PagI TCATGA 1 cut(s) 663
PaqCI CACCTGC 1 cut(s) 917
PciI ACATGT 1 cut(s) 522
PctI GAATGC 2 cut(s) 262, 738
PdmI GAANNNNTTC 2 cut(s) 197, 297
PfeI GAWTC 5 cut(s) 71, 256, 608, 631, 790
PkrI GCNGC 4 cut(s) 335, 427, 754, 757
PleI GAGTC 2 cut(s) 223, 545
PpsI GAGTC 2 cut(s) 223, 545
PscI ACATGT 1 cut(s) 522
Psp124BI GAGCTC 2 cut(s) 101, 659
Psp1406I AACGTT 1 cut(s) 296
Psp6I CCWGG 3 cut(s) 275, 284, 428
PspGI CCWGG 3 cut(s) 275, 284, 428
PspN4I GGNNCC 2 cut(s) 564, 708
PspPI GGNCC 1 cut(s) 273
PstNI CAGNNNCTG 1 cut(s) 500
RsaI GTAC 2 cut(s) 29, 365
RsaNI GTAC 2 cut(s) 28, 364
RseI CAYNNNNRTG 2 cut(s) 140, 800
SacI GAGCTC 2 cut(s) 101, 659
SaqAI TTAA 2 cut(s) 585, 963
SatI GCNGC 4 cut(s) 334, 426, 753, 756
Sau3AI GATC 3 cut(s) 9, 118, 303
Sau96I GGNCC 1 cut(s) 273
SchI GAGTC 2 cut(s) 224, 546
ScrFI CCNGG 4 cut(s) 76, 277, 286, 430
SduI GDGCHC 3 cut(s) 101, 567, 659
SfcI CTRYAG 1 cut(s) 921
SfuI TTCGAA 1 cut(s) 575
SinI GGWCC 1 cut(s) 273
SmiMI CAYNNNNRTG 2 cut(s) 140, 800
Sse9I AATT 5 cut(s) 447, 452, 557, 903, 935
SspMI CTAG 3 cut(s) 138, 837, 891
SstI GAGCTC 2 cut(s) 101, 659
StyD4I CCNGG 4 cut(s) 74, 275, 284, 428
StyI CCWWGG 3 cut(s) 234, 597, 731
TaaI ACNGT 2 cut(s) 623, 922
TaiI ACGT 1 cut(s) 299
TaqI TCGA 5 cut(s) 12, 95, 126, 218, 575
TaqII GACCGA 1 cut(s) 97
TasI AATT 5 cut(s) 447, 452, 557, 903, 935
TauI GCSGC 2 cut(s) 336, 428
TfiI GAWTC 5 cut(s) 71, 256, 608, 631, 790
Tru1I TTAA 2 cut(s) 585, 963
Tru9I TTAA 2 cut(s) 585, 963
TscAI CASTG 1 cut(s) 802
TseFI GTSAC 1 cut(s) 929
TseI GCWGC 2 cut(s) 752, 755
Tsp45I GTSAC 1 cut(s) 929
TspDTI ATGAA 3 cut(s) 652, 680, 821
TspGWI ACGGA 1 cut(s) 237
TspRI CASTG 1 cut(s) 802
VpaK11BI GGWCC 1 cut(s) 273
XagI CCTNNNNNAGG 1 cut(s) 818
XapI RAATTY 1 cut(s) 447
XceI RCATGY 1 cut(s) 526
XmiI GTMKAC 1 cut(s) 228
XmnI GAANNNNTTC 2 cut(s) 197, 297
XspI CTAG 3 cut(s) 138, 837, 891
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.