Basic Information

Type: gene

Biological Identity

Species

rosa_rugosa

Chromosome

GWHBQTZ00000005

Orthogroup ID

OG0012597

Physical Location & Seq

Genomic Coordinates Reverse (-)

34463272 .. 34470529

Gene Structure 7258 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rorug05G0298100.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2877 bp

ATGAAGCTGTTTTCAAATCCAATTGGGGCTTCATCGGTTGCCAAGGGAGATATTGGTATGAGTAGTAAGGGGGAAAGCTTTTCCAATCGGGTTGACATGGATATTGATGATGATAATGAATCTGTTGATTTGACTAGCATACATAATCTGGGAGAAGCTTTTTTGAAGGATTTCTGTAAGGAGGCATCAGTTTCATTCTTCAACCAGCATGCTCGGCTTCAAAATATGACATACTCAGCCAAGATCAAAGTGAACGTTACTGTTGAGGTATATACTCAAAATCTTGTCAGAAGTGAAAAGTTTAAAACTGGGAAAGAACAATGTCTTGACAAGCAGATTGTGACTACAGAGAGTAGAGATGTCTTTATTGGTAAGCTCCCAGTGATGGTGAAGTCTGACTTGTGTTGGATGAAAGACGAGAAAGCTGACTGTGAATTTGACCATGGAGGTTATTTTATAATCAAGGGTGCAGAGAAGACCTTTATTGCACAAGAGCAGAGCTGTCTGAAGAGACTTCTAATTACAAATAATCAGGGGATCACAGTAGCATATAGAAATCCCTGTCTGGATTTGGTTTTTTGCCCTTGGGTTTCTTCGGACAATGAAGTTACAGATCTGATTGATTATGGCAAAGAAGATGCTAGCATTTCAAGCATTCTAGTTGCCTCAATTCGTGATGCTGACCAACGAAAAGATAACTTCAGGAGAGGGAAGAATGCTCTCAGATATGTTGAAGAGATGATAAAGAATACCACGTTTCCTCCTCGGGAAAGCATTGAAGAATGCATCAGCATGTATCTGTTCCCCAATCTCAGGGGCATGAAGCAAAAGGCTCGGTTTCTTGGCTATATGGTGAAGTCTCTTTTACAGACCTTCAGAGGCCACAGGAAATGTGACAACAGAGAAGAATTGAAGAACAAGAGGTTTCACTTGGCTGGTGAGCTTCTAGAGCGAGAGCTGAAGGCACACGTAGGACATGCAGGAAGGCGCATGGCAAAGGCTTTGCAGAGAGACCTTTGTGGTGACCGTGTTGTCCGCCTGGTTGAGCAATATCTAGATGCTACAGTTGTAACTAATGGCATCTCCAGAGCATTCTCAACTGGAGCGTGGTGTCATCCTTTCAAGAGGATGGAGAGGATGTCTGGTGTGGTGGTAAATGTTGGGAAAACAAATCCACTGCAAACAATGGTAGATATGAGGAAGACACGCCTCCAAGTTCAATACACGGGGAAGGTCGGAGATGCCAGTACCTTCTCACTTATTTTATGTTTTGTTTCATGCTTTTCTTGCAATGACTGTCCCTGTCAATGGCATCCTTCTCACTGGGGTAGGGTTTGCTTTATCTCAACCCCAGATGGTGAGAATTGTGGGCTTGTGAAACATTTGGCTACCACTGCACTGGTAAGTACAAACATATTGGAGCCCATATTGCCCGACTTTTTCTCTTATGGCATGGAGGAGCTGGTAGATGATACTTCAACATCACTTCATGGGAAGAATAAAGTTTTTCTCAATGGGGATTGGGTTGGCGTTTGTGAAGATTCCCATGATTTTGTCACAAAACTAAGGACATTGAGACGCAGGAAAAAGTTGCTACATCAGGTGGAAATCAAAAGAGATGAGCAGCTCCTGGAAGTACGCATATATTCTGATGCCGGAAGGATTCTGCGTCCTTTCCTGTCTCAGAAGCACTCTCAGCGGGCCATTGGTTTTTCTACCACAAATCCTAACCACAGAGTGGATACTCTGTCTCACCAACTACACTACCCTCTGAGGCCGCTTTTTCGTACTATGGTATCTGATTGCCTTGGCCAACCAGGAAACCCAGTGAGCCATAACAGAGTTATGCCCAAGCCTGAATACTTTAATGGCCAAAATGCAATTGTTGCGGTCAATGTGCACCTAGGATATAACCAAGAAGATTCTATTGTGATGAACCGCGCATCTGTGCAACTTGGTGCATATCTGCAGAGTGGTGATCTTATTATTGGGAGGTGCAGTGAATCTGGTACTGATCATAGCATCAAACTGAGGCATACTGAAAGAGGAAAGGTTCAGAAGGTTGTACTTTCCTCTAATGAAGAGGGAAATAATTTTGGTGTTGTATCCTTAAGACAGGTTCCTTCTCCTTGCCTTGGAGACAAGTTCTCAAGCATGCATGGGCAGAAGGGTGTCCTAGGCTTTTTGGAGTCTGAAGAGAACTTCCCCTTCACAATACAGGGGATAGTTCCAGATATTGTAATTAATCCACATGCATTTCCTTCACGACAAGCTCCTGAGGCTGCTTTAGGGAAGGGGATTGCCTGTGGTGGCTCAATGAGATATGCAACTCCTTTCTCTACTCCCTCTGTTGACAACATCACAGATCAGCTTCATAGAGCTGGATTTCAAAGATGGGGAAATGAGAGAGTATACAGTGGTCAAACTGGTCAAATGGTTCGCCAACTGATATTTATGGGCCCAACATTCTATCAACGTCTCATTCACATGTCTGAAGACAAAGTGAAGTTCAGGAACACTGGGCCAGTTCACCCTCTCACCCGGCAGCCAGTGGCAGACAGGAAGAGATTTGGTGGGGTAAAGTTTGGTGAGATGGAGCGCGACTGCCTTATAGCCCATGGAGCATCAGCAAACTTACATGAACGCCTTTTCACTCTTAGTGACTCCTCCGAAATGCATGCTTGCCTGAAATGCAAAAATGCGGCTAATGTGATAGATGGAACAGTTGTTGGCAGTAGAATCAGGGGTCCATATTGCCTGGTCTGTAAGTCTGTTGATGACATTGTCAGGTTGAATGTTCCATATGGAGCCAAGTTATTGTGCCAGGAACTTTTCAGCATGGGCATAAGTCTCAAGTTTGAGACCAGGCTCTGCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000120 GO:0000500 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005667 GO:0005730 GO:0006355 GO:0006356 GO:0008150 GO:0009889 GO:0009891 GO:0009893 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0019219 GO:0019222 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031974 GO:0031981 GO:0032991 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044452 GO:0044464 GO:0044798 GO:0045893 GO:0045935 GO:0045943 GO:0048518 GO:0048522 GO:0050789 GO:0050794 GO:0051171 GO:0051173 GO:0051252 GO:0051254 GO:0060255 GO:0065007 GO:0070013 GO:0080090 GO:1902680 GO:1903506 GO:1903508 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DEK_C SWIB

Protein Analysis

958

Amino Acids

107.73

Weight (kDa)

8.79

Isoelectric Point (pI)

37.12

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
RNA_pol_Rpb2_1	PF04563	63 - 343	8.7e-23	RNA polymerase beta subunit
RNA_pol_Rpb2_3	PF04565	436 - 467	2.4e-12	RNA polymerase Rpb2, domain 3
RNA_pol_Rpb2_4	PF04566	502 - 560	4.8e-20	RNA polymerase Rpb2, domain 4
RNA_pol_Rpb2_6	PF00562	560 - 655	1.7e-15	RNA polymerase Rpb2, domain 6
RNA_pol_Rpb2_6	PF00562	667 - 856	2.4e-62	RNA polymerase Rpb2, domain 6
RNA_pol_Rpb2_7	PF04560	858 - 954	7.4e-25	RNA polymerase Rpb2, domain 7

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0012597)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_3g30330
malus_domestica	MD03G1190700.v1.1 MD11G1207400.v1.1
prunus_persica	Prupe.4G228000_v2.0.a1
pyrus_communis	pycom03g14390 pycom11g18060
rosa_chinensis	RchiOBHm_Chr5g0056201
rosa_laevigata	RLG00000035063
rosa_multiflora	Rmu_co7981676.1_g000001 Rmu_sc0000680.1_g000024
rosa_roxburghii	Rroxscaffold_1G00024090
rosa_rugosa	Rorug05G0298000 Rorug05G0298100
rosa_samantha	Rh5AG369100 Rh5BG380200 Rh5CG403000 Rh5DG393200
rosa_wichuraiana	Rw0G004420 Rw0G012660

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	458
AasI	GACNNNNNNGTC	1 cut(s)	1031
AccB7I	CCANNNNNTGG	1 cut(s)	1738
AccI	GTMKAC	1 cut(s)	2412
AccII	CGCG	2 cut(s)	1941, 2601
AciI	CCGC	6 cut(s)	1036, 1699, 1778, 1889, 1939, 2702
AclI	AACGTT	1 cut(s)	255
AclWI	GGATC	1 cut(s)	545
AcoI	YGGCCR	2 cut(s)	1810, 1870
AcsI	RAATTY	1 cut(s)	434
AcuI	CTGAAG	6 cut(s)	527, 685, 859, 980, 2214, 2514
AdeI	CACNNNGTG	1 cut(s)	1738
AfaI	GTAC	6 cut(s)	1249, 1408, 1638, 1789, 2011, 2067
AfiI	CCNNNNNNNGG	5 cut(s)	385, 813, 1308, 1738, 2135
AflII	CTTAAG	1 cut(s)	2110
AflIII	ACRYGT	2 cut(s)	967, 2487
AjnI	CCWGG	6 cut(s)	1038, 1629, 1816, 2757, 2823, 2864
AjuI	GAANNNNNNNTTGG	2 cut(s)	13, 45
Alw21I	GWGCWC	1 cut(s)	1902
Alw44I	GTGCAC	1 cut(s)	1898
AlwI	GGATC	1 cut(s)	545
AlwNI	CAGNNNCTG	2 cut(s)	1630, 2871
Ama87I	CYCGRG	1 cut(s)	765
AoxI	GGCC	7 cut(s)	880, 1701, 1775, 1810, 1870, 2458, 2522
ApaI	GGGCCC	1 cut(s)	2462
ApaLI	GTGCAC	1 cut(s)	1898
ApeKI	GCWGC	3 cut(s)	1624, 2282, 2545
ApoI	RAATTY	1 cut(s)	434
ArsI	GACNNNNNNTTYG	2 cut(s)	419, 451
AseI	ATTAAT	1 cut(s)	2244
Asp700I	GAANNNNTTC	1 cut(s)	170
AspA2I	CCTAGG	2 cut(s)	1903, 2176
AspLEI	GCGC	3 cut(s)	990, 1943, 2601
AspS9I	GGNCC	5 cut(s)	1701, 2458, 2459, 2522, 2747
AsuC2I	CCSGG	1 cut(s)	2542
AsuNHI	GCTAGC	1 cut(s)	641
AvaI	CYCGRG	1 cut(s)	765
AvaII	GGWCC	1 cut(s)	2747
AvrII	CCTAGG	2 cut(s)	1903, 2176
AxyI	CCTNAGG	1 cut(s)	2277
BaeGI	GKGCMC	2 cut(s)	1902, 2462
BaeI	ACNNNNGTAYC	2 cut(s)	1779, 1812
BalI	TGGCCA	2 cut(s)	1812, 1872
BanII	GRGCYC	2 cut(s)	1425, 2462
BbsI	GAAGAC	3 cut(s)	482, 1208, 2502
Bbv12I	GWGCWC	1 cut(s)	1902
BbvI	GCAGC	3 cut(s)	1636, 2269, 2557
BccI	CCATC	6 cut(s)	379, 1123, 1349, 2388, 2587, 2711
BcgI	CGANNNNNNTGC	4 cut(s)	815, 849, 2660, 2694
BciT130I	CCWGG	6 cut(s)	1040, 1631, 1818, 2759, 2825, 2866
BciVI	GTATCC	2 cut(s)	1735, 2116
BclI	TGATCA	1 cut(s)	2014
BcnI	CCSGG	1 cut(s)	2542
BfaI	CTAG	7 cut(s)	135, 642, 659, 947, 1055, 1904, 2177
BfmI	CTRYAG	3 cut(s)	345, 1062, 1967
BfrI	CTTAAG	1 cut(s)	2110
BfuI	GTATCC	2 cut(s)	1735, 2116
BglII	AGATCT	1 cut(s)	613
BisI	GCNGC	5 cut(s)	1625, 1778, 2283, 2546, 2703
BlnI	CCTAGG	2 cut(s)	1903, 2176
BlsI	GCNGC	5 cut(s)	1626, 1779, 2284, 2547, 2704
Bme1390I	CCNGG	7 cut(s)	1040, 1631, 1818, 2542, 2759, 2825, 2866
Bme18I	GGWCC	1 cut(s)	2747
BmeT110I	CYCGRG	1 cut(s)	765
BmgT120I	GGNCC	5 cut(s)	1701, 2458, 2459, 2522, 2747
BmiI	GGNNCC	5 cut(s)	1422, 2121, 2460, 2748, 2809
BmrFI	CCNGG	7 cut(s)	1040, 1631, 1818, 2542, 2759, 2825, 2866
BmrI	ACTGGG	5 cut(s)	318, 374, 1333, 1820, 2529
BmtI	GCTAGC	1 cut(s)	645
BmuI	ACTGGG	5 cut(s)	318, 374, 1333, 1820, 2529
BpiI	GAAGAC	3 cut(s)	482, 1208, 2502
BpmI	CTGGAG	2 cut(s)	1069, 1122
BpuEI	CTTGAG	2 cut(s)	2134, 2837
BpuMI	CCSGG	1 cut(s)	2542
BsaAI	YACGTR	1 cut(s)	970
BsaI	GGTCTC	2 cut(s)	1005, 2855
BsaJI	CCNNGG	9 cut(s)	42, 442, 584, 764, 1807, 1903, 2134, 2176, 2617
BsaXI	ACNNNNNCTCC	6 cut(s)	745, 775, 1124, 1154, 1984, 2014
Bsc4I	CCNNNNNNNGG	5 cut(s)	385, 813, 1308, 1738, 2135
Bse21I	CCTNAGG	1 cut(s)	2277
Bse3DI	GCAATG	1 cut(s)	1297
BseBI	CCWGG	6 cut(s)	1040, 1631, 1818, 2759, 2825, 2866
BseDI	CCNNGG	9 cut(s)	42, 442, 584, 764, 1807, 1903, 2134, 2176, 2617
BseGI	GGATG	5 cut(s)	414, 1114, 1134, 1143, 1312
BseLI	CCNNNNNNNGG	5 cut(s)	385, 813, 1308, 1738, 2135
BseMI	GCAATG	1 cut(s)	1297
BseMII	CTCAG	8 cut(s)	249, 736, 826, 1697, 1709, 1763, 2021, 2268
BseRI	GAGGAG	3 cut(s)	753, 1472, 2656
BseSI	GKGCMC	2 cut(s)	1902, 2462
BseXI	GCAGC	3 cut(s)	1636, 2269, 2557
BsgI	GTGCAG	3 cut(s)	489, 1380, 2017
Bsh1236I	CGCG	2 cut(s)	1941, 2601
BshFI	GGCC	7 cut(s)	882, 1703, 1777, 1812, 1872, 2460, 2524
BsiHKAI	GWGCWC	1 cut(s)	1902
BsiHKCI	CYCGRG	1 cut(s)	765
BsiSI	CCGG	2 cut(s)	1656, 2542
BslFI	GGGAC	1 cut(s)	1284
BslI	CCNNNNNNNGG	5 cut(s)	385, 813, 1308, 1738, 2135
BsmBI	CGTCTC	2 cut(s)	1570, 2483
BsmFI	GGGAC	1 cut(s)	1284
BsmI	GAATGC	4 cut(s)	654, 721, 788, 1091
BsnI	GGCC	7 cut(s)	882, 1703, 1777, 1812, 1872, 2460, 2524
Bso31I	GGTCTC	2 cut(s)	1005, 2855
BsoBI	CYCGRG	1 cut(s)	765
Bsp120I	GGGCCC	1 cut(s)	2458
Bsp1286I	GDGCHC	3 cut(s)	1425, 1902, 2462
Bsp143I	GATC	6 cut(s)	243, 537, 613, 1978, 2014, 2365
Bsp19I	CCATGG	2 cut(s)	442, 2617
BspACI	CCGC	6 cut(s)	1036, 1699, 1778, 1889, 1939, 2702
BspANI	GGCC	7 cut(s)	882, 1703, 1777, 1812, 1872, 2460, 2524
BspCNI	CTCAG	8 cut(s)	248, 735, 825, 1696, 1708, 1764, 2022, 2269
BspFNI	CGCG	2 cut(s)	1941, 2601
BspLI	GGNNCC	5 cut(s)	1422, 2121, 2460, 2748, 2809
BspMAI	CTGCAG	1 cut(s)	1971
BspOI	GCTAGC	1 cut(s)	645
BspPI	GGATC	1 cut(s)	545
BspTI	CTTAAG	1 cut(s)	2110
BspTNI	GGTCTC	2 cut(s)	1005, 2855
BsrDI	GCAATG	1 cut(s)	1297
BssECI	CCNNGG	9 cut(s)	42, 442, 584, 764, 1807, 1903, 2134, 2176, 2617
BssMI	GATC	6 cut(s)	243, 537, 613, 1978, 2014, 2365
BssNAI	GTATAC	1 cut(s)	2413
BssT1I	CCWWGG	8 cut(s)	42, 442, 584, 1807, 1903, 2134, 2176, 2617
Bst1107I	GTATAC	1 cut(s)	2413
Bst2UI	CCWGG	6 cut(s)	1040, 1631, 1818, 2759, 2825, 2866
Bst4CI	ACNGT	8 cut(s)	262, 431, 544, 1028, 1066, 1298, 2417, 2725
Bst6I	CTCTTC	5 cut(s)	503, 729, 2076, 2190, 2558
BstAFI	CTTAAG	1 cut(s)	2110
BstAPI	GCANNNNNTGC	1 cut(s)	1886
BstBAI	YACGTR	1 cut(s)	970
BstC8I	GCNNGC	6 cut(s)	210, 643, 1701, 2156, 2679, 2683
BstDSI	CCRYGG	2 cut(s)	442, 2617
BstEII	GGTNACC	1 cut(s)	1022
BstF5I	GGATG	5 cut(s)	414, 1114, 1134, 1143, 1312
BstFNI	CGCG	2 cut(s)	1941, 2601
BstHHI	GCGC	3 cut(s)	990, 1943, 2601
BstKTI	GATC	6 cut(s)	246, 540, 616, 1981, 2017, 2368
BstMBI	GATC	6 cut(s)	243, 537, 613, 1978, 2014, 2365
BstNI	CCWGG	6 cut(s)	1040, 1631, 1818, 2759, 2825, 2866
BstNSI	RCATGY	7 cut(s)	212, 796, 980, 2158, 2255, 2491, 2681
BstPI	GGTNACC	1 cut(s)	1022
BstSCI	CCNGG	7 cut(s)	1038, 1629, 1816, 2540, 2757, 2823, 2864
BstSFI	CTRYAG	3 cut(s)	345, 1062, 1967
BstSLI	GKGCMC	2 cut(s)	1902, 2462
BstUI	CGCG	2 cut(s)	1941, 2601
BstV1I	GCAGC	3 cut(s)	1636, 2269, 2557
BstV2I	GAAGAC	3 cut(s)	482, 1208, 2502
BstX2I	RGATCY	1 cut(s)	613
BstXI	CCANNNNNNTGG	1 cut(s)	1399
BstYI	RGATCY	1 cut(s)	613
BstZ17I	GTATAC	1 cut(s)	2413
Bsu36I	CCTNAGG	1 cut(s)	2277
BsuI	GTATCC	2 cut(s)	1735, 2116
BsuRI	GGCC	7 cut(s)	882, 1703, 1777, 1812, 1872, 2460, 2524
BtgI	CCRYGG	2 cut(s)	442, 2617
BtsCI	GGATG	5 cut(s)	414, 1114, 1134, 1143, 1312
BtsI	GCAGTG	3 cut(s)	1175, 1392, 2005
Cac8I	GCNNGC	6 cut(s)	210, 643, 1701, 2156, 2679, 2683
CaiI	CAGNNNCTG	2 cut(s)	1630, 2871
CfoI	GCGC	3 cut(s)	990, 1943, 2601
Cfr13I	GGNCC	5 cut(s)	1701, 2458, 2459, 2522, 2747
CseI	GACGC	2 cut(s)	1587, 1658
Csp6I	GTAC	6 cut(s)	1248, 1407, 1637, 1788, 2010, 2066
CviQI	GTAC	6 cut(s)	1248, 1407, 1637, 1788, 2010, 2066
DpnI	GATC	6 cut(s)	245, 539, 615, 1980, 2016, 2367
DpnII	GATC	6 cut(s)	243, 537, 613, 1978, 2014, 2365
DraI	TTTAAA	1 cut(s)	304
DraIII	CACNNNGTG	1 cut(s)	1738
DrdI	GACNNNNNNGTC	1 cut(s)	1031
DseDI	GACNNNNNNGTC	1 cut(s)	1031
EaeI	YGGCCR	2 cut(s)	1810, 1870
Eam1104I	CTCTTC	5 cut(s)	503, 729, 2076, 2190, 2558
EarI	CTCTTC	5 cut(s)	503, 729, 2076, 2190, 2558
EciI	GGCGGA	1 cut(s)	1025
Eco130I	CCWWGG	8 cut(s)	42, 442, 584, 1807, 1903, 2134, 2176, 2617
Eco24I	GRGCYC	2 cut(s)	1425, 2462
Eco31I	GGTCTC	2 cut(s)	1005, 2855
Eco47I	GGWCC	1 cut(s)	2747
Eco57I	CTGAAG	6 cut(s)	527, 685, 859, 980, 2214, 2514
Eco81I	CCTNAGG	1 cut(s)	2277
Eco88I	CYCGRG	1 cut(s)	765
Eco91I	GGTNACC	1 cut(s)	1022
EcoO65I	GGTNACC	1 cut(s)	1022
EcoRII	CCWGG	6 cut(s)	1038, 1629, 1816, 2757, 2823, 2864
EcoT14I	CCWWGG	8 cut(s)	42, 442, 584, 1807, 1903, 2134, 2176, 2617
EcoT22I	ATGCAT	4 cut(s)	788, 2160, 2257, 2679
EcoT38I	GRGCYC	2 cut(s)	1425, 2462
ErhI	CCWWGG	8 cut(s)	42, 442, 584, 1807, 1903, 2134, 2176, 2617
Esp3I	CGTCTC	2 cut(s)	1570, 2483
FalI	AAGNNNNNCTT	2 cut(s)	383, 415
FaqI	GGGAC	1 cut(s)	1284
FauI	CCCGC	1 cut(s)	1692
FauNDI	CATATG	1 cut(s)	2803
FbaI	TGATCA	1 cut(s)	2014
FblI	GTMKAC	1 cut(s)	2412
Fnu4HI	GCNGC	5 cut(s)	1625, 1778, 2283, 2546, 2703
FokI	GGATG	5 cut(s)	421, 1101, 1141, 1150, 1299
FriOI	GRGCYC	2 cut(s)	1425, 2462
Fsp4HI	GCNGC	5 cut(s)	1625, 1778, 2283, 2546, 2703
FspBI	CTAG	7 cut(s)	135, 642, 659, 947, 1055, 1904, 2177
GlaI	GCGC	3 cut(s)	989, 1942, 2600
GluI	GCNGC	5 cut(s)	1625, 1778, 2283, 2546, 2703
GsuI	CTGGAG	2 cut(s)	1069, 1122
HaeIII	GGCC	7 cut(s)	882, 1703, 1777, 1812, 1872, 2460, 2524
HapII	CCGG	2 cut(s)	1656, 2542
HgaI	GACGC	2 cut(s)	1587, 1658
HhaI	GCGC	3 cut(s)	990, 1943, 2601
Hin6I	GCGC	3 cut(s)	988, 1941, 2599
HinP1I	GCGC	3 cut(s)	988, 1941, 2599
HincII	GTYRAC	2 cut(s)	94, 2353
HindII	GTYRAC	2 cut(s)	94, 2353
HindIII	AAGCTT	2 cut(s)	76, 156
HinfI	GANTC	8 cut(s)	119, 1541, 1663, 1922, 2003, 2189, 2663, 2739
HpaII	CCGG	2 cut(s)	1656, 2542
Hpy166II	GTNNAC	6 cut(s)	94, 253, 1900, 2353, 2413, 2530
Hpy8I	GTNNAC	6 cut(s)	94, 253, 1900, 2353, 2413, 2530
HpyCH4III	ACNGT	8 cut(s)	262, 431, 544, 1028, 1066, 1298, 2417, 2725
HpyCH4IV	ACGT	4 cut(s)	255, 755, 969, 2476
HpySE526I	ACGT	4 cut(s)	255, 755, 969, 2476
HspAI	GCGC	3 cut(s)	988, 1941, 2599
Ksp22I	TGATCA	1 cut(s)	2014
Kzo9I	GATC	6 cut(s)	243, 537, 613, 1978, 2014, 2365
LmnI	GCTCC	9 cut(s)	381, 1103, 1420, 1459, 1632, 2278, 2596, 2621, 2807
Lsp1109I	GCAGC	3 cut(s)	1636, 2269, 2557
MaeI	CTAG	7 cut(s)	135, 642, 659, 947, 1055, 1904, 2177
MaeII	ACGT	4 cut(s)	255, 755, 969, 2476
MaeIII	GTNAC	8 cut(s)	256, 340, 607, 893, 1022, 1069, 1555, 2660
MalI	GATC	6 cut(s)	245, 539, 615, 1980, 2016, 2367
MboI	GATC	6 cut(s)	243, 537, 613, 1978, 2014, 2365
MfeI	CAATTG	2 cut(s)	21, 1881
MflI	RGATCY	1 cut(s)	613
MhlI	GDGCHC	3 cut(s)	1425, 1902, 2462
MlsI	TGGCCA	2 cut(s)	1812, 1872
MluCI	AATT	9 cut(s)	21, 434, 519, 669, 908, 1363, 1881, 2092, 2241
MluNI	TGGCCA	2 cut(s)	1812, 1872
MlyI	GAGTC	2 cut(s)	2198, 2657
MmeI	TCCRAC	2 cut(s)	386, 1216
Mox20I	TGGCCA	2 cut(s)	1812, 1872
Mph1103I	ATGCAT	4 cut(s)	788, 2160, 2257, 2679
MroXI	GAANNNNTTC	1 cut(s)	170
MscI	TGGCCA	2 cut(s)	1812, 1872
MseI	TTAA	4 cut(s)	303, 1866, 2111, 2244
MslI	CAYNNNNRTG	2 cut(s)	791, 2486
Msp20I	TGGCCA	2 cut(s)	1812, 1872
MspA1I	CMGCKG	1 cut(s)	1699
MspCI	CTTAAG	1 cut(s)	2110
MspI	CCGG	2 cut(s)	1656, 2542
MspR9I	CCNGG	7 cut(s)	1040, 1631, 1818, 2542, 2759, 2825, 2866
MunI	CAATTG	2 cut(s)	21, 1881
Mva1269I	GAATGC	4 cut(s)	654, 721, 788, 1091
MvaI	CCWGG	6 cut(s)	1040, 1631, 1818, 2759, 2825, 2866
MvnI	CGCG	2 cut(s)	1941, 2601
NciI	CCSGG	1 cut(s)	2542
NcoI	CCATGG	2 cut(s)	442, 2617
NdeI	CATATG	1 cut(s)	2803
NdeII	GATC	6 cut(s)	243, 537, 613, 1978, 2014, 2365
NheI	GCTAGC	1 cut(s)	641
NlaIV	GGNNCC	5 cut(s)	1422, 2121, 2460, 2748, 2809
NmeAIII	GCCGAG	1 cut(s)	193
NmuCI	GTSAC	5 cut(s)	340, 893, 1022, 1555, 2660
NsiI	ATGCAT	4 cut(s)	788, 2160, 2257, 2679
NspI	RCATGY	7 cut(s)	212, 796, 980, 2158, 2255, 2491, 2681
PaeI	GCATGC	3 cut(s)	212, 2158, 2681
PciI	ACATGT	1 cut(s)	2487
PctI	GAATGC	4 cut(s)	654, 721, 788, 1091
PdmI	GAANNNNTTC	1 cut(s)	170
PfeI	GAWTC	6 cut(s)	119, 1541, 1663, 1922, 2003, 2739
PflFI	GACNNNGTC	1 cut(s)	2783
PflMI	CCANNNNNTGG	1 cut(s)	1738
PfoI	TCCNGGA	1 cut(s)	1629
PkrI	GCNGC	5 cut(s)	1626, 1779, 2284, 2547, 2704
PleI	GAGTC	2 cut(s)	2197, 2657
PpsI	GAGTC	2 cut(s)	2197, 2657
Ppu21I	YACGTR	1 cut(s)	970
PscI	ACATGT	1 cut(s)	2487
PshBI	ATTAAT	1 cut(s)	2244
PsiI	TTATAA	1 cut(s)	458
Psp1406I	AACGTT	1 cut(s)	255
Psp6I	CCWGG	6 cut(s)	1038, 1629, 1816, 2757, 2823, 2864
PspEI	GGTNACC	1 cut(s)	1022
PspGI	CCWGG	6 cut(s)	1038, 1629, 1816, 2757, 2823, 2864
PspN4I	GGNNCC	5 cut(s)	1422, 2121, 2460, 2748, 2809
PspOMI	GGGCCC	1 cut(s)	2458
PspPI	GGNCC	5 cut(s)	1701, 2458, 2459, 2522, 2747
PstI	CTGCAG	1 cut(s)	1971
PstNI	CAGNNNCTG	2 cut(s)	1630, 2871
PsuI	RGATCY	1 cut(s)	613
PsyI	GACNNNGTC	1 cut(s)	2783
RsaI	GTAC	6 cut(s)	1249, 1408, 1638, 1789, 2011, 2067
RsaNI	GTAC	6 cut(s)	1248, 1407, 1637, 1788, 2010, 2066
RseI	CAYNNNNRTG	2 cut(s)	791, 2486
SaqAI	TTAA	4 cut(s)	303, 1866, 2111, 2244
SatI	GCNGC	5 cut(s)	1625, 1778, 2283, 2546, 2703
Sau3AI	GATC	6 cut(s)	243, 537, 613, 1978, 2014, 2365
Sau96I	GGNCC	5 cut(s)	1701, 2458, 2459, 2522, 2747
SchI	GAGTC	2 cut(s)	2198, 2657
ScrFI	CCNGG	7 cut(s)	1040, 1631, 1818, 2542, 2759, 2825, 2866
SduI	GDGCHC	3 cut(s)	1425, 1902, 2462
SfcI	CTRYAG	3 cut(s)	345, 1062, 1967
SinI	GGWCC	1 cut(s)	2747
SmiMI	CAYNNNNRTG	2 cut(s)	791, 2486
SmlI	CTYRAG	3 cut(s)	2110, 2149, 2852
SmoI	CTYRAG	3 cut(s)	2110, 2149, 2852
SphI	GCATGC	3 cut(s)	212, 2158, 2681
Sse9I	AATT	9 cut(s)	21, 434, 519, 669, 908, 1363, 1881, 2092, 2241
SsiI	CCGC	6 cut(s)	1036, 1699, 1778, 1889, 1939, 2702
SspMI	CTAG	7 cut(s)	135, 642, 659, 947, 1055, 1904, 2177
StyD4I	CCNGG	7 cut(s)	1038, 1629, 1816, 2540, 2757, 2823, 2864
StyI	CCWWGG	8 cut(s)	42, 442, 584, 1807, 1903, 2134, 2176, 2617
TaaI	ACNGT	8 cut(s)	262, 431, 544, 1028, 1066, 1298, 2417, 2725
TaiI	ACGT	4 cut(s)	258, 758, 972, 2479
TasI	AATT	9 cut(s)	21, 434, 519, 669, 908, 1363, 1881, 2092, 2241
TatI	WGTACW	2 cut(s)	1406, 2065
TauI	GCSGC	2 cut(s)	1780, 2705
TfiI	GAWTC	6 cut(s)	119, 1541, 1663, 1922, 2003, 2739
Tru1I	TTAA	4 cut(s)	303, 1866, 2111, 2244
Tru9I	TTAA	4 cut(s)	303, 1866, 2111, 2244
TseFI	GTSAC	5 cut(s)	340, 893, 1022, 1555, 2660
TseI	GCWGC	3 cut(s)	1624, 2282, 2545
Tsp45I	GTSAC	5 cut(s)	340, 893, 1022, 1555, 2660
Tth111I	GACNNNGTC	1 cut(s)	2783
Van91I	CCANNNNNTGG	1 cut(s)	1738
Vha464I	CTTAAG	1 cut(s)	2110
VneI	GTGCAC	1 cut(s)	1898
VpaK11BI	GGWCC	1 cut(s)	2747
VspI	ATTAAT	1 cut(s)	2244
XapI	RAATTY	1 cut(s)	434
XbaI	TCTAGA	2 cut(s)	946, 1054
XceI	RCATGY	7 cut(s)	212, 796, 980, 2158, 2255, 2491, 2681
XmaJI	CCTAGG	2 cut(s)	1903, 2176
XmiI	GTMKAC	1 cut(s)	2412
XmnI	GAANNNNTTC	1 cut(s)	170
XspI	CTAG	7 cut(s)	135, 642, 659, 947, 1055, 1904, 2177
Zsp2I	ATGCAT	4 cut(s)	788, 2160, 2257, 2679

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.