Basic Information

Type: gene

Biological Identity

Species

rosa_rugosa

Chromosome

GWHBQTZ00000005

Orthogroup ID

OG0003131

Physical Location & Seq

Genomic Coordinates Forward (+)

67895136 .. 67900024

Gene Structure 4889 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rorug05G0497000.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1692 bp

ATGCCTTCCATCGCCGACCGCAGCGTCGAACTCGAATCCAACGCCGTCGGATCCGCCGGAGTTTCTACTCCTCCGAAACGGAGGCTGAGATCCAATTCGTCGCCGATGAAGCTGAGTCCGATGAAATGCAAGCGCGTCGGAATTTCTTCCGTCGCGAAATTACGCAGCGTTGTGGTCGAATCGGAGAGGAGCGTGGAGCAGGTAGTCGGATCCGGCGGTGATTTGACTCCTCAGAAGCGGAAGTTGAGGTCCGATTCGTCGTCGCCGATGAAGCCGAGACCGGTCTCGTCGCCGATGAAGTGTAAATCGGCTCGCCGGAACCTCAGTGCTTGCACCAGCACTCCTGCAAATGGAATTGAGAAGTATTTCAGTCAGAAAAAGCCTCAAGCCTTTTGCAAACCACCGGTTGTTGCTAAGCCGAATTGGAATCCCAGAGATAAGGAGCAGATGAGGATAGTGAAGGAGGCACTGCACTTGTCAACGGTCCCCTCTACTGTTGCGTGCCGCGATGTGGAGCAGAAGAGGGTTCTGGAGTTCTGCAAGTCATGCATAGAGCAAGAGAAGGCTGGGAGTTTATATGTATGCGGGTGTCCTGGGACCGGAAAGTCCTTGTCCATGGAGAAAGTGAAACAGCTTTTGGTGAATTGGGCAGGAGAGGGTGGATTTCAGGAACCGGATGTGTTGGAATTGAACTGTACATCGCTAACAAAAACTTCTGATATTTTCACTAAGATACTTGGTAAATACCAACCTCAAAAGAAAGCTAATGGCACTACCTCCCTGCAACTTCTTCAGAAATTATATTCTCAAAAGCCTCAGTCAGATGGGACTAAGATGATGTTGATAATAGTTGATGAGTTGGATTACTTAATCACTAAAGACCGGGCAGTGCTCCATGACCTTTTCATGCTCACAACATACCCATTCTCAAGATGTATACTTTTAGGAGTAGCAAATGCAATCGACTTAGCTGATCGTTTTGTTCCAAAACTTCAGTCATTAAATTGTAAGCCTATGGTTATAACTTTTCGGGCATACTCTAAGGATCAGATTCTCAAGATTCTCCAAGAGAGGCTTATGGCACTACCTTACACTGTTTTTCAACCACATGCATTGGAACTTTGTGCCAGAAAAGTTGCTGCTGCATCTGGAGATATGAGGAAAGCTCTCTGTATTTCCAGGAGTGCAATTGAGATGCTGGAAGCAGAGTTGAGAGAATCTACTGACAAGTTAAATTCATCAACAGCAGAGAATCCATTCTCTGAGCAACAAACAGCTCCAGCTCTTGCTCTGGTGAAAAAACAGGAGATTGATATTGTGAGAATTGATCACATGGCCATTGCTTTGTCAAAGACATTCAAGTCAGCAGTAGTGGATACCATACAATGTCTTCCTCAACATCAACAGATTATAATGTGCTCTGCTGCAAAGCATTTCCGTGGTGTGAAGAAAGATACAACTATAGGAGAGTTAAACAAATCTTACGTAGACATCTGCAAAGCAACACTAATTCCCCGGGTTGGGAGTTTTGAATTTTCAAGTATGTGCAAAGTGCTAAATGACCAGGGCCTCCTCAAACTCAGTGGCCAATCCAGAGATATAAAATCCAAGAGAGTGACATTGAATGTAGATGAAGGGGACATCACATTTGCATTGCAGGGAATCCGGTTTTTTCGGACTTGTCTTGAGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

CX9C

Protein Analysis

563

Amino Acids

62.37

Weight (kDa)

9.43

Isoelectric Point (pI)

47.96

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
AAA_22	PF13401	188 - 305	1.6e-13	AAA domain
Cdc6-ORC-like_ATPase_lid	PF22606	346 - 403	2.1e-11	Cdc6/ORC-like, ATPase lid domain
WHD_Cdc6	PF09079	459 - 551	1.4e-19	CDC6, C terminal winged helix domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003131)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G47278 AT1G47278 AT1G47278
fragaria_vesca	FvH4_3g35770 FvH4_3g35770 FvH4_3g35770
malus_domestica	MD02G1271300.v1.1 MD11G1111500.v1.1
prunus_persica	Prupe.6G079900_v2.0.a1
pyrus_communis	pycom11g09210
rosa_chinensis	RchiOBHm_Chr5g0006711 RchiOBHm_Chr5g0063941
rosa_laevigata	RLG00000031419
rosa_multiflora	Rmu_sc0011986.1_g000001 Rmu_sc0017347.1_g000004 Rmu_sc0018387.1_g000007
rosa_roxburghii	Rroxscaffold_1G00016840 Rroxscaffold_1G00069310
rosa_rugosa	Rorug04G0423800 Rorug04G0423900 Rorug05G0361100 Rorug05G0496800 Rorug05G0496900 Rorug05G0497000 Rorug05G0497100
rosa_samantha	Rh5AG051700 Rh5AG419800 Rh5BG435600 Rh5CG059200 Rh5CG458700 Rh5DG049700 Rh5DG448800 Rh6AG006000 Rh6CG004200 Rh6DG004100
rosa_wichuraiana	Rw5G005280 Rw5G039450

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	1022, 1412
AasI	GACNNNNNNGTC	3 cut(s)	23, 604, 1360
Acc36I	ACCTGC	1 cut(s)	190
AccI	GTMKAC	2 cut(s)	937, 1488
AccII	CGCG	3 cut(s)	135, 155, 507
AciI	CCGC	6 cut(s)	19, 54, 216, 238, 505, 585
AclWI	GGATC	6 cut(s)	45, 58, 84, 204, 217, 1053
AcoI	YGGCCR	2 cut(s)	1335, 1585
AcsI	RAATTY	3 cut(s)	141, 1234, 1532
AcuI	CTGAAG	2 cut(s)	776, 977
AfaI	GTAC	1 cut(s)	697
AfiI	CCNNNNNNNGG	5 cut(s)	350, 511, 599, 1520, 1521
AgeI	ACCGGT	2 cut(s)	280, 403
AgsI	TTSAA	6 cut(s)	691, 1103, 1360, 1532, 1539, 1624
AjnI	CCWGG	3 cut(s)	592, 1178, 1563
AjuI	GAANNNNNNNTTGG	2 cut(s)	620, 652
AluBI	AGCT	7 cut(s)	112, 634, 764, 971, 1166, 1277, 1283
AluI	AGCT	7 cut(s)	112, 634, 764, 971, 1166, 1277, 1283
Alw21I	GWGCWC	2 cut(s)	894, 1421
Alw26I	GTCTC	2 cut(s)	271, 289
AlwI	GGATC	6 cut(s)	45, 58, 84, 204, 217, 1053
Ama87I	CYCGRG	1 cut(s)	1515
AoxI	GGCC	3 cut(s)	1335, 1567, 1585
ApeKI	GCWGC	5 cut(s)	21, 165, 1139, 1142, 1424
ApoI	RAATTY	3 cut(s)	141, 1234, 1532
ArsI	GACNNNNNNTTYG	4 cut(s)	980, 1012, 1631, 1663
AsiGI	ACCGGT	2 cut(s)	280, 403
Asp700I	GAANNNNTTC	3 cut(s)	145, 365, 1256
AspLEI	GCGC	1 cut(s)	135
AspS9I	GGNCC	4 cut(s)	249, 484, 597, 1567
AsuC2I	CCSGG	3 cut(s)	884, 1516, 1517
AsuHPI	GGTGA	3 cut(s)	230, 652, 1306
AvaI	CYCGRG	1 cut(s)	1515
AvaII	GGWCC	3 cut(s)	249, 484, 597
BalI	TGGCCA	2 cut(s)	1337, 1587
BamHI	GGATCC	2 cut(s)	50, 209
BbsI	GAAGAC	1 cut(s)	1382
Bbv12I	GWGCWC	2 cut(s)	894, 1421
BbvI	GCAGC	5 cut(s)	33, 177, 1126, 1129, 1411
BccI	CCATC	2 cut(s)	17, 818
BceAI	ACGGC	1 cut(s)	29
BcgI	CGANNNNNNTGC	2 cut(s)	118, 152
BciT130I	CCWGG	3 cut(s)	594, 1180, 1565
BciVI	GTATCC	1 cut(s)	1369
BclI	TGATCA	1 cut(s)	1327
BcnI	CCSGG	3 cut(s)	884, 1516, 1517
BcoDI	GTCTC	2 cut(s)	271, 289
BfmI	CTRYAG	1 cut(s)	1461
BfuAI	ACCTGC	1 cut(s)	190
BfuI	GTATCC	1 cut(s)	1369
BisI	GCNGC	6 cut(s)	22, 166, 505, 1140, 1143, 1425
BlpI	GCTNAGC	1 cut(s)	414
BlsI	GCNGC	6 cut(s)	23, 167, 506, 1141, 1144, 1426
Bme1390I	CCNGG	6 cut(s)	594, 884, 1180, 1516, 1517, 1565
Bme18I	GGWCC	3 cut(s)	249, 484, 597
BmeT110I	CYCGRG	1 cut(s)	1515
BmgT120I	GGNCC	4 cut(s)	249, 484, 597, 1567
BmiI	GGNNCC	6 cut(s)	52, 211, 320, 486, 598, 672
BmrFI	CCNGG	6 cut(s)	594, 884, 1180, 1516, 1517, 1565
BmsI	GCATC	2 cut(s)	1154, 1185
BpiI	GAAGAC	1 cut(s)	1382
BplI	GAGNNNNNCTC	2 cut(s)	1150, 1182
BpmI	CTGGAG	3 cut(s)	551, 1170, 1263
Bpu1102I	GCTNAGC	1 cut(s)	414
BpuEI	CTTGAG	3 cut(s)	369, 913, 1040
BpuMI	CCSGG	3 cut(s)	884, 1516, 1517
BsaAI	YACGTR	1 cut(s)	1486
BsaI	GGTCTC	2 cut(s)	271, 289
BsaJI	CCNNGG	6 cut(s)	593, 615, 1438, 1514, 1515, 1564
BsaWI	WCCGGW	5 cut(s)	280, 403, 599, 673, 1665
BsaXI	ACNNNNNCTCC	2 cut(s)	325, 355
Bsc4I	CCNNNNNNNGG	5 cut(s)	350, 511, 599, 1520, 1521
Bse118I	RCCGGY	2 cut(s)	280, 403
Bse3DI	GCAATG	2 cut(s)	1338, 1652
BseBI	CCWGG	3 cut(s)	594, 1180, 1565
BseDI	CCNNGG	6 cut(s)	593, 615, 1438, 1514, 1515, 1564
BseGI	GGATG	1 cut(s)	682
BseLI	CCNNNNNNNGG	5 cut(s)	350, 511, 599, 1520, 1521
BseMI	GCAATG	2 cut(s)	1338, 1652
BseMII	CTCAG	7 cut(s)	77, 104, 245, 337, 830, 1254, 1594
BseRI	GAGGAG	4 cut(s)	60, 202, 219, 1562
BseXI	GCAGC	5 cut(s)	33, 177, 1126, 1129, 1411
BseYI	CCCAGC	1 cut(s)	566
BsgI	GTGCAG	1 cut(s)	455
Bsh1236I	CGCG	3 cut(s)	135, 155, 507
Bsh1285I	CGRYCG	1 cut(s)	19
BshFI	GGCC	3 cut(s)	1337, 1569, 1587
BshTI	ACCGGT	2 cut(s)	280, 403
BsiEI	CGRYCG	1 cut(s)	19
BsiHKAI	GWGCWC	2 cut(s)	894, 1421
BsiHKCI	CYCGRG	1 cut(s)	1515
BslFI	GGGAC	4 cut(s)	470, 610, 841, 1652
BslI	CCNNNNNNNGG	5 cut(s)	350, 511, 599, 1520, 1521
BsmAI	GTCTC	2 cut(s)	271, 289
BsmFI	GGGAC	4 cut(s)	470, 610, 841, 1652
BsnI	GGCC	3 cut(s)	1337, 1569, 1587
Bso31I	GGTCTC	2 cut(s)	271, 289
BsoBI	CYCGRG	1 cut(s)	1515
Bsp1286I	GDGCHC	2 cut(s)	894, 1421
Bsp1407I	TGTACA	1 cut(s)	695
Bsp143I	GATC	6 cut(s)	50, 89, 209, 973, 1045, 1327
Bsp1720I	GCTNAGC	1 cut(s)	414
Bsp19I	CCATGG	1 cut(s)	615
Bsp68I	TCGCGA	1 cut(s)	155
BspACI	CCGC	6 cut(s)	19, 54, 216, 238, 505, 585
BspANI	GGCC	3 cut(s)	1337, 1569, 1587
BspCNI	CTCAG	7 cut(s)	78, 105, 244, 336, 829, 1255, 1593
BspFNI	CGCG	3 cut(s)	135, 155, 507
BspLI	GGNNCC	6 cut(s)	52, 211, 320, 486, 598, 672
BspMI	ACCTGC	1 cut(s)	190
BspPI	GGATC	6 cut(s)	45, 58, 84, 204, 217, 1053
BspTNI	GGTCTC	2 cut(s)	271, 289
BsrDI	GCAATG	2 cut(s)	1338, 1652
BsrFI	RCCGGY	2 cut(s)	280, 403
BsrGI	TGTACA	1 cut(s)	695
BssAI	RCCGGY	2 cut(s)	280, 403
BssECI	CCNNGG	6 cut(s)	593, 615, 1438, 1514, 1515, 1564
BssMI	GATC	6 cut(s)	50, 89, 209, 973, 1045, 1327
BssNAI	GTATAC	1 cut(s)	938
BssT1I	CCWWGG	1 cut(s)	615
Bst1107I	GTATAC	1 cut(s)	938
Bst2UI	CCWGG	3 cut(s)	594, 1180, 1565
Bst4CI	ACNGT	4 cut(s)	484, 496, 695, 1096
Bst6I	CTCTTC	1 cut(s)	515
BstAPI	GCANNNNNTGC	1 cut(s)	546
BstAUI	TGTACA	1 cut(s)	695
BstBAI	YACGTR	1 cut(s)	1486
BstC8I	GCNNGC	4 cut(s)	131, 313, 331, 502
BstDSI	CCRYGG	2 cut(s)	615, 1438
BstF5I	GGATG	1 cut(s)	682
BstFNI	CGCG	3 cut(s)	135, 155, 507
BstHHI	GCGC	1 cut(s)	135
BstKTI	GATC	6 cut(s)	53, 92, 212, 976, 1048, 1330
BstMAI	GTCTC	2 cut(s)	271, 289
BstMBI	GATC	6 cut(s)	50, 89, 209, 973, 1045, 1327
BstMCI	CGRYCG	1 cut(s)	19
BstMWI	GCNNNNNNNGC	3 cut(s)	109, 271, 546
BstNI	CCWGG	3 cut(s)	594, 1180, 1565
BstNSI	RCATGY	1 cut(s)	1112
BstSCI	CCNGG	6 cut(s)	592, 882, 1178, 1514, 1515, 1563
BstSFI	CTRYAG	1 cut(s)	1461
BstSNI	TACGTA	1 cut(s)	1486
BstUI	CGCG	3 cut(s)	135, 155, 507
BstV1I	GCAGC	5 cut(s)	33, 177, 1126, 1129, 1411
BstV2I	GAAGAC	1 cut(s)	1382
BstX2I	RGATCY	3 cut(s)	50, 89, 209
BstYI	RGATCY	3 cut(s)	50, 89, 209
BstZ17I	GTATAC	1 cut(s)	938
BsuI	GTATCC	1 cut(s)	1369
BsuRI	GGCC	3 cut(s)	1337, 1569, 1587
BtgI	CCRYGG	2 cut(s)	615, 1438
BtgZI	GCGATG	2 cut(s)	522, 684
BtsCI	GGATG	1 cut(s)	682
BtsI	GCAGTG	2 cut(s)	467, 894
BtsIMutI	CAGTG	5 cut(s)	331, 467, 894, 1092, 1588
BtuMI	TCGCGA	1 cut(s)	155
BveI	ACCTGC	1 cut(s)	190
Cac8I	GCNNGC	4 cut(s)	131, 313, 331, 502
CfoI	GCGC	1 cut(s)	135
Cfr10I	RCCGGY	2 cut(s)	280, 403
Cfr13I	GGNCC	4 cut(s)	249, 484, 597, 1567
Cfr9I	CCCGGG	1 cut(s)	1515
CseI	GACGC	2 cut(s)	13, 124
Csp6I	GTAC	1 cut(s)	696
CspAI	ACCGGT	2 cut(s)	280, 403
CspCI	CAANNNNNGTGG	4 cut(s)	1095, 1130, 1564, 1599
CviAII	CATG	6 cut(s)	546, 616, 896, 907, 1109, 1333
CviQI	GTAC	1 cut(s)	696
DpnI	GATC	6 cut(s)	52, 91, 211, 975, 1047, 1329
DpnII	GATC	6 cut(s)	50, 89, 209, 973, 1045, 1327
DrdI	GACNNNNNNGTC	3 cut(s)	23, 604, 1360
DseDI	GACNNNNNNGTC	3 cut(s)	23, 604, 1360
EaeI	YGGCCR	2 cut(s)	1335, 1585
Eam1104I	CTCTTC	1 cut(s)	515
EarI	CTCTTC	1 cut(s)	515
EciI	GGCGGA	1 cut(s)	43
Eco105I	TACGTA	1 cut(s)	1486
Eco130I	CCWWGG	1 cut(s)	615
Eco31I	GGTCTC	2 cut(s)	271, 289
Eco47I	GGWCC	3 cut(s)	249, 484, 597
Eco57I	CTGAAG	2 cut(s)	776, 977
Eco88I	CYCGRG	1 cut(s)	1515
EcoO109I	RGGNCCY	1 cut(s)	1567
EcoRII	CCWGG	3 cut(s)	592, 1178, 1563
EcoT14I	CCWWGG	1 cut(s)	615
EcoT22I	ATGCAT	2 cut(s)	551, 1114
ErhI	CCWWGG	1 cut(s)	615
FaeI	CATG	6 cut(s)	549, 619, 899, 910, 1112, 1336
FalI	AAGNNNNNCTT	2 cut(s)	1059, 1091
FaqI	GGGAC	4 cut(s)	470, 610, 841, 1652
FatI	CATG	6 cut(s)	545, 615, 895, 906, 1108, 1332
FauI	CCCGC	1 cut(s)	578
FbaI	TGATCA	1 cut(s)	1327
FblI	GTMKAC	2 cut(s)	937, 1488
Fnu4HI	GCNGC	6 cut(s)	22, 166, 505, 1140, 1143, 1425
FokI	GGATG	1 cut(s)	689
Fsp4HI	GCNGC	6 cut(s)	22, 166, 505, 1140, 1143, 1425
GlaI	GCGC	1 cut(s)	134
GluI	GCNGC	6 cut(s)	22, 166, 505, 1140, 1143, 1425
GsaI	CCCAGC	1 cut(s)	570
GsuI	CTGGAG	3 cut(s)	551, 1170, 1263
HaeIII	GGCC	3 cut(s)	1337, 1569, 1587
HgaI	GACGC	2 cut(s)	13, 124
HhaI	GCGC	1 cut(s)	135
Hin1II	CATG	6 cut(s)	549, 619, 899, 910, 1112, 1336
Hin6I	GCGC	1 cut(s)	133
HinP1I	GCGC	1 cut(s)	133
HincII	GTYRAC	1 cut(s)	480
HindII	GTYRAC	1 cut(s)	480
HphI	GGTGA	3 cut(s)	230, 652, 1306
Hpy166II	GTNNAC	3 cut(s)	480, 938, 1489
Hpy188III	TCNNGA	8 cut(s)	154, 530, 668, 930, 1057, 1149, 1593, 1685
Hpy8I	GTNNAC	3 cut(s)	480, 938, 1489
Hpy99I	CGWCG	8 cut(s)	29, 50, 103, 140, 155, 262, 265, 292
HpyAV	CCTTC	4 cut(s)	15, 454, 556, 1628
HpyCH4III	ACNGT	4 cut(s)	484, 496, 695, 1096
HpyCH4IV	ACGT	1 cut(s)	1485
HpyF10VI	GCNNNNNNNGC	3 cut(s)	109, 271, 546
HpySE526I	ACGT	1 cut(s)	1485
Hsp92II	CATG	6 cut(s)	549, 619, 899, 910, 1112, 1336
HspAI	GCGC	1 cut(s)	133
Ksp22I	TGATCA	1 cut(s)	1327
Kzo9I	GATC	6 cut(s)	50, 89, 209, 973, 1045, 1327
LmnI	GCTCC	6 cut(s)	189, 196, 442, 514, 897, 1282
Lsp1109I	GCAGC	5 cut(s)	33, 177, 1126, 1129, 1411
LweI	GCATC	2 cut(s)	1154, 1185
MaeII	ACGT	1 cut(s)	1485
MaeIII	GTNAC	1 cut(s)	1615
MalI	GATC	6 cut(s)	52, 91, 211, 975, 1047, 1329
MboI	GATC	6 cut(s)	50, 89, 209, 973, 1045, 1327
MboII	GAAGA	5 cut(s)	138, 532, 782, 1382, 1459
MfeI	CAATTG	1 cut(s)	1188
MflI	RGATCY	3 cut(s)	50, 89, 209
MhlI	GDGCHC	2 cut(s)	894, 1421
MlsI	TGGCCA	2 cut(s)	1337, 1587
MluNI	TGGCCA	2 cut(s)	1337, 1587
MlyI	GAGTC	2 cut(s)	124, 220
MmeI	TCCRAC	6 cut(s)	28, 63, 118, 187, 663, 840
Mox20I	TGGCCA	2 cut(s)	1337, 1587
Mph1103I	ATGCAT	2 cut(s)	551, 1114
MroXI	GAANNNNTTC	3 cut(s)	145, 365, 1256
MscI	TGGCCA	2 cut(s)	1337, 1587
MseI	TTAA	4 cut(s)	869, 1001, 1232, 1472
MslI	CAYNNNNRTG	1 cut(s)	1437
Msp20I	TGGCCA	2 cut(s)	1337, 1587
MspR9I	CCNGG	6 cut(s)	594, 884, 1180, 1516, 1517, 1565
MunI	CAATTG	1 cut(s)	1188
MvaI	CCWGG	3 cut(s)	594, 1180, 1565
MvnI	CGCG	3 cut(s)	135, 155, 507
MwoI	GCNNNNNNNGC	3 cut(s)	109, 271, 546
NciI	CCSGG	3 cut(s)	884, 1516, 1517
NcoI	CCATGG	1 cut(s)	615
NdeII	GATC	6 cut(s)	50, 89, 209, 973, 1045, 1327
NlaIII	CATG	6 cut(s)	549, 619, 899, 910, 1112, 1336
NlaIV	GGNNCC	6 cut(s)	52, 211, 320, 486, 598, 672
NmeAIII	GCCGAG	1 cut(s)	300
NmuCI	GTSAC	1 cut(s)	1615
NruI	TCGCGA	1 cut(s)	155
NsiI	ATGCAT	2 cut(s)	551, 1114
NspI	RCATGY	1 cut(s)	1112
PcsI	WCGNNNNNNNCGW	1 cut(s)	263
PdmI	GAANNNNTTC	3 cut(s)	145, 365, 1256
PfeI	GAWTC	9 cut(s)	35, 179, 254, 427, 1051, 1060, 1217, 1252, 1662
PfoI	TCCNGGA	1 cut(s)	1178
PinAI	ACCGGT	2 cut(s)	280, 403
PkrI	GCNGC	6 cut(s)	23, 167, 506, 1141, 1144, 1426
PleI	GAGTC	2 cut(s)	123, 220
PpsI	GAGTC	2 cut(s)	123, 220
Ppu21I	YACGTR	1 cut(s)	1486
PsiI	TTATAA	2 cut(s)	1022, 1412
Psp6I	CCWGG	3 cut(s)	592, 1178, 1563
PspFI	CCCAGC	1 cut(s)	566
PspGI	CCWGG	3 cut(s)	592, 1178, 1563
PspN4I	GGNNCC	6 cut(s)	52, 211, 320, 486, 598, 672
PspPI	GGNCC	4 cut(s)	249, 484, 597, 1567
PsuI	RGATCY	3 cut(s)	50, 89, 209
RruI	TCGCGA	1 cut(s)	155
RsaI	GTAC	1 cut(s)	697
RsaNI	GTAC	1 cut(s)	696
RseI	CAYNNNNRTG	1 cut(s)	1437
SaqAI	TTAA	4 cut(s)	869, 1001, 1232, 1472
SatI	GCNGC	6 cut(s)	22, 166, 505, 1140, 1143, 1425
Sau3AI	GATC	6 cut(s)	50, 89, 209, 973, 1045, 1327
Sau96I	GGNCC	4 cut(s)	249, 484, 597, 1567
SchI	GAGTC	2 cut(s)	124, 220
ScrFI	CCNGG	6 cut(s)	594, 884, 1180, 1516, 1517, 1565
SduI	GDGCHC	2 cut(s)	894, 1421
SfaNI	GCATC	2 cut(s)	1154, 1185
SfcI	CTRYAG	1 cut(s)	1461
SinI	GGWCC	3 cut(s)	249, 484, 597
SmaI	CCCGGG	1 cut(s)	1517
SmiMI	CAYNNNNRTG	1 cut(s)	1437
SmlI	CTYRAG	4 cut(s)	384, 928, 1055, 1685
SmoI	CTYRAG	4 cut(s)	384, 928, 1055, 1685
SnaBI	TACGTA	1 cut(s)	1486
SsiI	CCGC	6 cut(s)	19, 54, 216, 238, 505, 585
StyD4I	CCNGG	6 cut(s)	592, 882, 1178, 1514, 1515, 1563
StyI	CCWWGG	1 cut(s)	615
TaaI	ACNGT	4 cut(s)	484, 496, 695, 1096
TaiI	ACGT	1 cut(s)	1488
TaqI	TCGA	4 cut(s)	27, 33, 177, 963
TatI	WGTACW	1 cut(s)	695
TauI	GCSGC	1 cut(s)	507
TfiI	GAWTC	9 cut(s)	35, 179, 254, 427, 1051, 1060, 1217, 1252, 1662
Tru1I	TTAA	4 cut(s)	869, 1001, 1232, 1472
Tru9I	TTAA	4 cut(s)	869, 1001, 1232, 1472
TscAI	CASTG	5 cut(s)	331, 474, 894, 1099, 1588
TseFI	GTSAC	1 cut(s)	1615
TseI	GCWGC	5 cut(s)	21, 165, 1139, 1142, 1424
Tsp45I	GTSAC	1 cut(s)	1615
TspDTI	ATGAA	7 cut(s)	122, 137, 284, 311, 895, 1227, 1647
TspGWI	ACGGA	3 cut(s)	94, 139, 1427
TspMI	CCCGGG	1 cut(s)	1515
TspRI	CASTG	5 cut(s)	331, 474, 894, 1099, 1588
VpaK11BI	GGWCC	3 cut(s)	249, 484, 597
XapI	RAATTY	3 cut(s)	141, 1234, 1532
XceI	RCATGY	1 cut(s)	1112
XmaI	CCCGGG	1 cut(s)	1515
XmiI	GTMKAC	2 cut(s)	937, 1488
XmnI	GAANNNNTTC	3 cut(s)	145, 365, 1256
Zsp2I	ATGCAT	2 cut(s)	551, 1114

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.