Rorug05G0528500

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0003510
Physical Location & Seq
Forward (+)
71776630 .. 71777031
402 bp
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UTR
Exon/CDS
Intron
Rorug05G0528500.1

Sequence Viewer

Length: 402 bp
ATGAAAATTGCAGTAGAAGGTTGCATGCATGGCGACCTCGACAATGTCTACGGGACCCTCCAATACATCGAAAGAAGGCACAACACCAAAATCGACCTCCTCCTCTGCTGCGGCGACTTTCAGGCCGTCAGGAATGAACACGATTTGAAGAGCTTAAAGGTGTCTCCAGAATACCGGAAGATGAACTCCTTTTGGAAGTATTATTCCGGCGAGGCCATCGCTCCCTATCCCACCATATTCATCGGCGGCAACCACGAAGCTTCTAACTATCTCTGGGAACTCTACTACGGAGGTTGGGCTGCTCCCAACATTTTCTTCTTAGGGTTTGCTGGGTCGTCAAGTTCGGAAACCTTCGCATTGGTGGCCTCTCTGGTATTTATAAACCGGAAGACTATAGTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

133

Amino Acids

15.1

Weight (kDa)

6.4

Isoelectric Point (pI)

45.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Metallophos PF00149 1 - 104 2.6e-09 Calcineurin-like phosphoesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 380
AccI GTMKAC 1 cut(s) 48
AciI CCGC 2 cut(s) 111, 246
AgsI TTSAA 1 cut(s) 148
AjuI GAANNNNNNNTTGG 2 cut(s) 299, 331
AluBI AGCT 2 cut(s) 153, 260
AluI AGCT 2 cut(s) 153, 260
Alw26I GTCTC 1 cut(s) 168
AoxI GGCC 3 cut(s) 123, 213, 363
ApeKI GCWGC 2 cut(s) 108, 299
AspS9I GGNCC 1 cut(s) 54
AvaII GGWCC 1 cut(s) 54
BbsI GAAGAC 1 cut(s) 395
BbvI GCAGC 2 cut(s) 95, 286
BccI CCATC 1 cut(s) 224
BceAI ACGGC 1 cut(s) 110
BcoDI GTCTC 1 cut(s) 168
BfmI CTRYAG 1 cut(s) 393
BisI GCNGC 4 cut(s) 109, 112, 247, 300
BlsI GCNGC 4 cut(s) 110, 113, 248, 301
Bme18I GGWCC 1 cut(s) 54
BmgT120I GGNCC 1 cut(s) 54
BmiI GGNNCC 2 cut(s) 55, 56
BpiI GAAGAC 1 cut(s) 395
BpmI CTGGAG 1 cut(s) 150
BsaWI WCCGGW 2 cut(s) 174, 384
BseRI GAGGAG 2 cut(s) 89, 92
BseXI GCAGC 2 cut(s) 95, 286
BseYI CCCAGC 1 cut(s) 329
BshFI GGCC 3 cut(s) 125, 215, 365
BsiSI CCGG 3 cut(s) 175, 207, 385
BslFI GGGAC 1 cut(s) 67
BsmAI GTCTC 1 cut(s) 168
BsmFI GGGAC 1 cut(s) 67
BsnI GGCC 3 cut(s) 125, 215, 365
BspACI CCGC 2 cut(s) 111, 246
BspANI GGCC 3 cut(s) 125, 215, 365
BspLI GGNNCC 2 cut(s) 55, 56
BspQI GCTCTTC 1 cut(s) 143
Bst6I CTCTTC 1 cut(s) 143
BstC8I GCNNGC 1 cut(s) 26
BstDEI CTNAG 1 cut(s) 319
BstMAI GTCTC 1 cut(s) 168
BstMWI GCNNNNNNNGC 2 cut(s) 30, 362
BstNSI RCATGY 1 cut(s) 28
BstSFI CTRYAG 1 cut(s) 393
BstV1I GCAGC 2 cut(s) 95, 286
BstV2I GAAGAC 1 cut(s) 395
BsuRI GGCC 3 cut(s) 125, 215, 365
BtgZI GCGATG 1 cut(s) 202
Cac8I GCNNGC 1 cut(s) 26
Cfr13I GGNCC 1 cut(s) 54
CviAII CATG 2 cut(s) 25, 29
CviJI RGCY 6 cut(s) 125, 153, 215, 260, 299, 365
CviKI_1 RGCY 6 cut(s) 125, 153, 215, 260, 299, 365
DdeI CTNAG 1 cut(s) 319
Eam1104I CTCTTC 1 cut(s) 143
EarI CTCTTC 1 cut(s) 143
Eco47I GGWCC 1 cut(s) 54
EcoO109I RGGNCCY 1 cut(s) 54
EcoT22I ATGCAT 1 cut(s) 30
FaeI CATG 2 cut(s) 28, 32
FaiI YATR 5 cut(s) 26, 30, 236, 380, 395
FaqI GGGAC 1 cut(s) 67
FatI CATG 2 cut(s) 24, 28
FblI GTMKAC 1 cut(s) 48
Fnu4HI GCNGC 4 cut(s) 109, 112, 247, 300
Fsp4HI GCNGC 4 cut(s) 109, 112, 247, 300
GluI GCNGC 4 cut(s) 109, 112, 247, 300
GsaI CCCAGC 1 cut(s) 333
GsuI CTGGAG 1 cut(s) 150
HaeIII GGCC 3 cut(s) 125, 215, 365
HapII CCGG 3 cut(s) 175, 207, 385
Hin1II CATG 2 cut(s) 28, 32
HindIII AAGCTT 1 cut(s) 258
HpaII CCGG 3 cut(s) 175, 207, 385
Hpy166II GTNNAC 1 cut(s) 49
Hpy188I TCNGA 1 cut(s) 346
Hpy188III TCNNGA 2 cut(s) 130, 167
Hpy8I GTNNAC 1 cut(s) 49
HpyAV CCTTC 3 cut(s) 11, 69, 361
HpyCH4V TGCA 3 cut(s) 11, 24, 28
HpyF10VI GCNNNNNNNGC 2 cut(s) 30, 362
HpyF3I CTNAG 1 cut(s) 319
Hsp92II CATG 2 cut(s) 28, 32
KflI GGGWCCC 1 cut(s) 54
LguI GCTCTTC 1 cut(s) 143
LmnI GCTCC 2 cut(s) 226, 307
LpnPI CCDG 9 cut(s) 107, 115, 180, 188, 220, 259, 315, 356, 398
Lsp1109I GCAGC 2 cut(s) 95, 286
MboII GAAGA 4 cut(s) 160, 190, 307, 400
MluCI AATT 1 cut(s) 6
MnlI CCTC 8 cut(s) 47, 68, 107, 110, 113, 205, 284, 376
Mph1103I ATGCAT 1 cut(s) 30
MseI TTAA 1 cut(s) 155
MspI CCGG 3 cut(s) 175, 207, 385
MwoI GCNNNNNNNGC 2 cut(s) 30, 362
NlaIII CATG 2 cut(s) 28, 32
NlaIV GGNNCC 2 cut(s) 55, 56
NsiI ATGCAT 1 cut(s) 30
NspI RCATGY 1 cut(s) 28
PaeI GCATGC 1 cut(s) 28
PciSI GCTCTTC 1 cut(s) 143
PflFI GACNNNGTC 1 cut(s) 44
PkrI GCNGC 4 cut(s) 110, 113, 248, 301
PpuMI RGGWCCY 1 cut(s) 54
PsiI TTATAA 1 cut(s) 380
Psp5II RGGWCCY 1 cut(s) 54
PspFI CCCAGC 1 cut(s) 329
PspN4I GGNNCC 2 cut(s) 55, 56
PspPI GGNCC 1 cut(s) 54
PspPPI RGGWCCY 1 cut(s) 54
PsyI GACNNNGTC 1 cut(s) 44
SapI GCTCTTC 1 cut(s) 143
SaqAI TTAA 1 cut(s) 155
SatI GCNGC 4 cut(s) 109, 112, 247, 300
Sau96I GGNCC 1 cut(s) 54
SetI ASST 8 cut(s) 22, 39, 99, 155, 162, 262, 295, 353
SfcI CTRYAG 1 cut(s) 393
SinI GGWCC 1 cut(s) 54
SphI GCATGC 1 cut(s) 28
Sse9I AATT 1 cut(s) 6
SsiI CCGC 2 cut(s) 111, 246
TaqI TCGA 3 cut(s) 39, 69, 93
TasI AATT 1 cut(s) 6
TauI GCSGC 2 cut(s) 114, 249
Tru1I TTAA 1 cut(s) 155
Tru9I TTAA 1 cut(s) 155
TseI GCWGC 2 cut(s) 108, 299
TspDTI ATGAA 4 cut(s) 17, 150, 197, 229
TspGWI ACGGA 1 cut(s) 303
Tth111I GACNNNGTC 1 cut(s) 44
VpaK11BI GGWCC 1 cut(s) 54
XceI RCATGY 1 cut(s) 28
XmiI GTMKAC 1 cut(s) 48
Zsp2I ATGCAT 1 cut(s) 30
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.