Rh1AG149300

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1A
OG0003510
Physical Location & Seq
Reverse (-)
28112487 .. 28115751
3265 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1AG149300.1

Sequence Viewer

Length: 1590 bp
ATGTTGAAGAAGCCGCCAAGTAGGAATCACAGAACCAAACGAATCAAGGTGAAGCATATTTTGCAGATTGCCCTGCTCCTTGGGGTTTGCTTTTGGTTGCTTTATCAGCTCAAGCATTCCCATGATAAGAAATTGGCGTTGGATGAGAAAGCTGCAAAAGCCTCAATCAGATCCCTGAGTGAAGGTGAGCTTCTGAAACTTGGGAGGAAAGACCTTCCTCACAAGGACGTCAGTAAAAATGTGAAACAAGAGGAAGAGGAGGAAGAAGAAACTGCAGGAGACGAAGAAGAGAAGCATGAGGTGGAAGGGCGAGAAGAAGAAGATCAAAAACATGAAGTAGAAGAGCGAGAAGAAGAGGATCTGAAGCATGATGTAGAAGAGCAAGAGGAGGAGGAAAGCAAGAATGATGATACAGAAGATGAGGGAGGAGCTGGAGATGATGAGATAGATGAAAATGAGCAAGAGAGAAAAGAAGGAAAAATTGACCATGAAGAAGACATTTTAGATGAAGAGAGAGAAAGAGAAGATGAGGGTGATGAGAAGGATGGTGACCGGAATGAGAGGGAAGAGAGAGAGGGTCAAGCAGAAAATGATAACTCATCAGACGATCAAGACAATGACGGAGGTGATAAGAATGCTCATGAGGCACGAGAGGAACATTACAAGGGGGATGATGCTTCTAGTGCAGTGACCCATGATGCCCAAGTTATAAGCACTGAAACTGAGAAAGTAAGTTCAGAAAGTGGAAATGAGAACTCTGGAAGCAGCTCTCTGGAAAAGGAGATAAAATCTAATTACACTGAGGATGTAAATGGAAACCAAAACAATTCAAGATTGTTGGATGGTAGTACGGCCGAAAATGGTACTGTAAGTCTGGCTGCAAGTGAAGAGAAAGTTGATGAAAATTTGTCCAATCCTGTGGACAGCTCAGTTTTAAATGCAACTATGAAAGTACAGTCCGATGATATAACAGAAGCAGACAATAACTCGACAAATGTGAGTACAGAATCGAGCAACAACTCACTGGAAGTCAACCAGGCAAATGGAACAGAAACTGTATCTGTGTCAGCTCACTCTCAAAATGGTACATTGGATGGTATGACTACTAGAGAGGGAATTGCCGGAGAAACCTTGGTTGTGGAACAGACTAATAACACAATTTCTGATAATTATCACTCAGATTCTAATTCAACAGTTTCTAGTAATAAGGTAGAGAGTAATGTGTCCGAAAAGATCTTAAGATCTAATGGCACAGCTCAACTGGAGGATGACTCCAGGTCTTCAACAATAAATGAGTCTACAGATGCCACAAAGAATGACGAATTGAAGAATGAAGATGCCAACAAGAATGAGGAAATAAAGAATGAAGAGGCCACCAAGAACAAATACTTGAATGGCACAAGTGAATCTGGCAAGACTGGCAACAGTTCAGACTCTAGAAATGGGACTGAGGATGCAGTTCAGCATGACCCCATCGATTCTTCTGATTCCCTCGTCTCTGAAGACAAGAAAGAGGCTCACACAGAGTTAGATACATTGCCAGAGATGAAAACAGAGGGCAATGAGAATGGACAGGCTGCAGCAGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

529

Amino Acids

58.51

Weight (kDa)

4.37

Isoelectric Point (pI)

49.39

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 710
AatII GACGTC 1 cut(s) 231
AccI GTMKAC 1 cut(s) 1298
AciI CCGC 1 cut(s) 14
AclWI GGATC 2 cut(s) 165, 366
AcoI YGGCCR 1 cut(s) 852
AcsI RAATTY 1 cut(s) 904
AcuI CTGAAG 2 cut(s) 383, 1521
AcyI GRCGYC 1 cut(s) 228
AfaI GTAC 5 cut(s) 850, 865, 954, 1003, 1087
AflII CTTAAG 1 cut(s) 1237
AgsI TTSAA 6 cut(s) 7, 831, 1191, 1284, 1327, 1393
AhdI GACNNNNNGTC 1 cut(s) 1276
AjnI CCWGG 2 cut(s) 1035, 1274
AjuI GAANNNNNNNTTGG 2 cut(s) 122, 154
AluBI AGCT 8 cut(s) 109, 152, 190, 431, 768, 927, 1070, 1256
AluI AGCT 8 cut(s) 109, 152, 190, 431, 768, 927, 1070, 1256
Alw26I GTCTC 2 cut(s) 273, 1501
AlwI GGATC 2 cut(s) 165, 366
AlwNI CAGNNNCTG 1 cut(s) 1055
AoxI GGCC 2 cut(s) 852, 1371
ApeKI GCWGC 5 cut(s) 152, 765, 878, 1577, 1580
ApoI RAATTY 1 cut(s) 904
AsuHPI GGTGA 5 cut(s) 61, 197, 545, 560, 638
BauI CACGAG 1 cut(s) 648
BbsI GAAGAC 3 cut(s) 501, 1272, 1509
BbvI GCAGC 4 cut(s) 139, 777, 865, 1564
BccI CCATC 4 cut(s) 539, 836, 1088, 1481
BceAI ACGGC 1 cut(s) 867
BciT130I CCWGG 2 cut(s) 1037, 1276
BcoDI GTCTC 2 cut(s) 273, 1501
BfaI CTAG 4 cut(s) 681, 1107, 1200, 1437
BfmI CTRYAG 3 cut(s) 273, 1299, 1578
BfrI CTTAAG 1 cut(s) 1237
BglII AGATCT 2 cut(s) 1233, 1241
BisI GCNGC 6 cut(s) 14, 153, 766, 879, 1578, 1581
BlsI GCNGC 6 cut(s) 15, 154, 767, 880, 1579, 1582
Bme1390I CCNGG 2 cut(s) 1037, 1276
BmeRI GACNNNNNGTC 1 cut(s) 1276
BmrFI CCNGG 2 cut(s) 1037, 1276
BmsI GCATC 5 cut(s) 664, 688, 1294, 1327, 1444
BpiI GAAGAC 3 cut(s) 501, 1272, 1509
BplI GAGNNNNNCTC 2 cut(s) 1256, 1288
BpmI CTGGAG 3 cut(s) 453, 1258, 1283
BpuEI CTTGAG 1 cut(s) 95
Bsa29I ATCGAT 1 cut(s) 1476
BsaBI GATNNNNATC 1 cut(s) 1170
BsaHI GRCGYC 1 cut(s) 228
BsaJI CCNNGG 2 cut(s) 79, 1131
BsaWI WCCGGW 1 cut(s) 552
Bse1I ACTGG 3 cut(s) 1029, 1266, 1423
Bse3DI GCAATG 2 cut(s) 1535, 1567
Bse8I GATNNNNATC 1 cut(s) 1170
BseBI CCWGG 2 cut(s) 1037, 1276
BseCI ATCGAT 1 cut(s) 1476
BseDI CCNNGG 2 cut(s) 79, 1131
BseGI GGATG 8 cut(s) 148, 550, 676, 811, 847, 1099, 1273, 1459
BseJI GATNNNNATC 1 cut(s) 1170
BseMI GCAATG 2 cut(s) 1535, 1567
BseMII CTCAG 6 cut(s) 167, 714, 792, 942, 1191, 1440
BseNI ACTGG 3 cut(s) 1029, 1266, 1423
BseRI GAGGAG 4 cut(s) 272, 401, 404, 441
BseX3I CGGCCG 1 cut(s) 852
BseXI GCAGC 4 cut(s) 139, 777, 865, 1564
BsgI GTGCAG 1 cut(s) 705
Bsh1285I CGRYCG 1 cut(s) 855
BshFI GGCC 2 cut(s) 854, 1373
BshVI ATCGAT 1 cut(s) 1476
BsiEI CGRYCG 1 cut(s) 855
BsiSI CCGG 2 cut(s) 553, 1122
BslFI GGGAC 1 cut(s) 1459
BsmAI GTCTC 2 cut(s) 273, 1501
BsmBI CGTCTC 2 cut(s) 273, 1501
BsmFI GGGAC 1 cut(s) 1459
BsmI GAATGC 2 cut(s) 115, 640
BsnI GGCC 2 cut(s) 854, 1373
Bsp143I GATC 6 cut(s) 170, 322, 358, 607, 1233, 1241
BspACI CCGC 1 cut(s) 14
BspANI GGCC 2 cut(s) 854, 1373
BspCNI CTCAG 6 cut(s) 168, 715, 793, 941, 1190, 1441
BspDI ATCGAT 1 cut(s) 1476
BspHI TCATGA 1 cut(s) 640
BspMAI CTGCAG 2 cut(s) 277, 1582
BspPI GGATC 2 cut(s) 165, 366
BspQI GCTCTTC 2 cut(s) 336, 372
BspTI CTTAAG 1 cut(s) 1237
BsrDI GCAATG 2 cut(s) 1535, 1567
BsrI ACTGG 3 cut(s) 1029, 1266, 1423
BssECI CCNNGG 2 cut(s) 79, 1131
BssMI GATC 6 cut(s) 170, 322, 358, 607, 1233, 1241
BssNI GRCGYC 1 cut(s) 228
BssSI CACGAG 1 cut(s) 648
BssT1I CCWWGG 2 cut(s) 79, 1131
Bst2BI CACGAG 1 cut(s) 648
Bst2UI CCWGG 2 cut(s) 1037, 1276
Bst4CI ACNGT 5 cut(s) 868, 957, 1057, 1195, 1427
Bst6I CTCTTC 9 cut(s) 249, 282, 336, 348, 372, 504, 561, 882, 1362
BstACI GRCGYC 1 cut(s) 228
BstAFI CTTAAG 1 cut(s) 1237
BstAPI GCANNNNNTGC 1 cut(s) 61
BstDEI CTNAG 6 cut(s) 176, 723, 801, 928, 1177, 1449
BstEII GGTNACC 1 cut(s) 548
BstF5I GGATG 8 cut(s) 148, 550, 676, 811, 847, 1099, 1273, 1459
BstKTI GATC 6 cut(s) 173, 325, 361, 610, 1236, 1244
BstMAI GTCTC 2 cut(s) 273, 1501
BstMBI GATC 6 cut(s) 170, 322, 358, 607, 1233, 1241
BstMCI CGRYCG 1 cut(s) 855
BstMWI GCNNNNNNNGC 6 cut(s) 61, 106, 158, 644, 683, 1419
BstNI CCWGG 2 cut(s) 1037, 1276
BstPI GGTNACC 1 cut(s) 548
BstSCI CCNGG 2 cut(s) 1035, 1274
BstSFI CTRYAG 3 cut(s) 273, 1299, 1578
BstV1I GCAGC 4 cut(s) 139, 777, 865, 1564
BstV2I GAAGAC 3 cut(s) 501, 1272, 1509
BstX2I RGATCY 4 cut(s) 170, 358, 1233, 1241
BstXI CCANNNNNNTGG 2 cut(s) 919, 1043
BstYI RGATCY 4 cut(s) 170, 358, 1233, 1241
BstZI CGGCCG 1 cut(s) 852
Bsu15I ATCGAT 1 cut(s) 1476
BsuRI GGCC 2 cut(s) 854, 1373
BsuTUI ATCGAT 1 cut(s) 1476
BtsCI GGATG 8 cut(s) 148, 550, 676, 811, 847, 1099, 1273, 1459
BtsI GCAGTG 1 cut(s) 693
BtsIMutI CAGTG 4 cut(s) 693, 714, 798, 1022
CaiI CAGNNNCTG 1 cut(s) 1055
CciI TCATGA 1 cut(s) 640
ClaI ATCGAT 1 cut(s) 1476
Csp6I GTAC 5 cut(s) 849, 864, 953, 1002, 1086
CviAII CATG 8 cut(s) 122, 296, 332, 368, 488, 641, 695, 1466
CviQI GTAC 5 cut(s) 849, 864, 953, 1002, 1086
DdeI CTNAG 6 cut(s) 176, 723, 801, 928, 1177, 1449
DpnI GATC 6 cut(s) 172, 324, 360, 609, 1235, 1243
DpnII GATC 6 cut(s) 170, 322, 358, 607, 1233, 1241
DraI TTTAAA 1 cut(s) 936
DriI GACNNNNNGTC 1 cut(s) 1276
EaeI YGGCCR 1 cut(s) 852
EagI CGGCCG 1 cut(s) 852
Eam1104I CTCTTC 9 cut(s) 249, 282, 336, 348, 372, 504, 561, 882, 1362
Eam1105I GACNNNNNGTC 1 cut(s) 1276
EarI CTCTTC 9 cut(s) 249, 282, 336, 348, 372, 504, 561, 882, 1362
EclXI CGGCCG 1 cut(s) 852
Eco130I CCWWGG 2 cut(s) 79, 1131
Eco52I CGGCCG 1 cut(s) 852
Eco57I CTGAAG 2 cut(s) 383, 1521
Eco91I GGTNACC 1 cut(s) 548
EcoO65I GGTNACC 1 cut(s) 548
EcoRII CCWGG 2 cut(s) 1035, 1274
EcoT14I CCWWGG 2 cut(s) 79, 1131
ErhI CCWWGG 2 cut(s) 79, 1131
Esp3I CGTCTC 2 cut(s) 273, 1501
FaeI CATG 8 cut(s) 125, 299, 335, 371, 491, 644, 698, 1469
FalI AAGNNNNNCTT 2 cut(s) 174, 206
FaqI GGGAC 1 cut(s) 1459
FatI CATG 8 cut(s) 121, 295, 331, 367, 487, 640, 694, 1465
FblI GTMKAC 1 cut(s) 1298
Fnu4HI GCNGC 6 cut(s) 14, 153, 766, 879, 1578, 1581
FokI GGATG 8 cut(s) 155, 557, 683, 818, 854, 1106, 1280, 1466
Fsp4HI GCNGC 6 cut(s) 14, 153, 766, 879, 1578, 1581
FspBI CTAG 4 cut(s) 681, 1107, 1200, 1437
GluI GCNGC 6 cut(s) 14, 153, 766, 879, 1578, 1581
GsuI CTGGAG 3 cut(s) 453, 1258, 1283
HaeIII GGCC 2 cut(s) 854, 1373
HapII CCGG 2 cut(s) 553, 1122
Hin1I GRCGYC 1 cut(s) 228
Hin1II CATG 8 cut(s) 125, 299, 335, 371, 491, 644, 698, 1469
HincII GTYRAC 1 cut(s) 1033
HindII GTYRAC 1 cut(s) 1033
HpaII CCGG 2 cut(s) 553, 1122
HphI GGTGA 5 cut(s) 61, 197, 545, 560, 638
Hpy166II GTNNAC 3 cut(s) 922, 1033, 1299
Hpy188III TCNNGA 6 cut(s) 611, 641, 759, 773, 831, 1437
Hpy8I GTNNAC 3 cut(s) 922, 1033, 1299
HpyAV CCTTC 5 cut(s) 176, 224, 299, 467, 535
HpyCH4III ACNGT 5 cut(s) 868, 957, 1057, 1195, 1427
HpyCH4IV ACGT 1 cut(s) 228
HpyCH4V TGCA 8 cut(s) 64, 155, 275, 686, 881, 941, 1457, 1580
HpyF10VI GCNNNNNNNGC 6 cut(s) 61, 106, 158, 644, 683, 1419
HpyF3I CTNAG 6 cut(s) 176, 723, 801, 928, 1177, 1449
HpySE526I ACGT 1 cut(s) 228
Hsp92I GRCGYC 1 cut(s) 228
Hsp92II CATG 8 cut(s) 125, 299, 335, 371, 491, 644, 698, 1469
Kzo9I GATC 6 cut(s) 170, 322, 358, 607, 1233, 1241
LguI GCTCTTC 2 cut(s) 336, 372
LmnI GCTCC 2 cut(s) 81, 428
Lsp1109I GCAGC 4 cut(s) 139, 777, 865, 1564
LweI GCATC 5 cut(s) 664, 688, 1294, 1327, 1444
MaeI CTAG 4 cut(s) 681, 1107, 1200, 1437
MaeII ACGT 1 cut(s) 228
MaeIII GTNAC 2 cut(s) 548, 688
MalI GATC 6 cut(s) 172, 324, 360, 609, 1235, 1243
MboI GATC 6 cut(s) 170, 322, 358, 607, 1233, 1241
MflI RGATCY 4 cut(s) 170, 358, 1233, 1241
MlyI GAGTC 3 cut(s) 1265, 1304, 1427
MmeI TCCRAC 2 cut(s) 120, 819
MseI TTAA 2 cut(s) 935, 1238
MslI CAYNNNNRTG 1 cut(s) 120
MspCI CTTAAG 1 cut(s) 1237
MspI CCGG 2 cut(s) 553, 1122
MspR9I CCNGG 2 cut(s) 1037, 1276
Mva1269I GAATGC 2 cut(s) 115, 640
MvaI CCWGG 2 cut(s) 1037, 1276
MwoI GCNNNNNNNGC 6 cut(s) 61, 106, 158, 644, 683, 1419
NdeII GATC 6 cut(s) 170, 322, 358, 607, 1233, 1241
NlaIII CATG 8 cut(s) 125, 299, 335, 371, 491, 644, 698, 1469
NmuCI GTSAC 2 cut(s) 548, 688
PagI TCATGA 1 cut(s) 640
PciSI GCTCTTC 2 cut(s) 336, 372
PctI GAATGC 2 cut(s) 115, 640
PfeI GAWTC 7 cut(s) 25, 42, 1007, 1181, 1406, 1478, 1487
PkrI GCNGC 6 cut(s) 15, 154, 767, 880, 1579, 1582
PleI GAGTC 3 cut(s) 1265, 1303, 1427
PpsI GAGTC 3 cut(s) 1265, 1303, 1427
PsiI TTATAA 1 cut(s) 710
Psp6I CCWGG 2 cut(s) 1035, 1274
PspEI GGTNACC 1 cut(s) 548
PspGI CCWGG 2 cut(s) 1035, 1274
PstI CTGCAG 2 cut(s) 277, 1582
PstNI CAGNNNCTG 1 cut(s) 1055
PsuI RGATCY 4 cut(s) 170, 358, 1233, 1241
RsaI GTAC 5 cut(s) 850, 865, 954, 1003, 1087
RsaNI GTAC 5 cut(s) 849, 864, 953, 1002, 1086
RseI CAYNNNNRTG 1 cut(s) 120
SapI GCTCTTC 2 cut(s) 336, 372
SaqAI TTAA 2 cut(s) 935, 1238
SatI GCNGC 6 cut(s) 14, 153, 766, 879, 1578, 1581
Sau3AI GATC 6 cut(s) 170, 322, 358, 607, 1233, 1241
SchI GAGTC 3 cut(s) 1265, 1304, 1427
ScrFI CCNGG 2 cut(s) 1037, 1276
SfaNI GCATC 5 cut(s) 664, 688, 1294, 1327, 1444
SfcI CTRYAG 3 cut(s) 273, 1299, 1578
SmiMI CAYNNNNRTG 1 cut(s) 120
SmlI CTYRAG 2 cut(s) 110, 1237
SmoI CTYRAG 2 cut(s) 110, 1237
SsiI CCGC 1 cut(s) 14
SspMI CTAG 4 cut(s) 681, 1107, 1200, 1437
StyD4I CCNGG 2 cut(s) 1035, 1274
StyI CCWWGG 2 cut(s) 79, 1131
TaaI ACNGT 5 cut(s) 868, 957, 1057, 1195, 1427
TaiI ACGT 1 cut(s) 231
TaqI TCGA 3 cut(s) 989, 1010, 1476
TatI WGTACW 2 cut(s) 952, 1001
TauI GCSGC 1 cut(s) 16
TfiI GAWTC 7 cut(s) 25, 42, 1007, 1181, 1406, 1478, 1487
Tru1I TTAA 2 cut(s) 935, 1238
Tru9I TTAA 2 cut(s) 935, 1238
TscAI CASTG 4 cut(s) 693, 721, 805, 1029
TseFI GTSAC 2 cut(s) 548, 688
TseI GCWGC 5 cut(s) 152, 765, 878, 1577, 1580
Tsp45I GTSAC 2 cut(s) 548, 688
TspDTI ATGAA 9 cut(s) 348, 465, 504, 522, 915, 962, 1347, 1380, 1562
TspGWI ACGGA 1 cut(s) 636
TspRI CASTG 4 cut(s) 693, 721, 805, 1029
Vha464I CTTAAG 1 cut(s) 1237
XapI RAATTY 1 cut(s) 904
XbaI TCTAGA 1 cut(s) 1436
XmiI GTMKAC 1 cut(s) 1298
XspI CTAG 4 cut(s) 681, 1107, 1200, 1437
ZraI GACGTC 1 cut(s) 229
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.