Rorug05G0530400

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0004736
Physical Location & Seq
Forward (+)
71903846 .. 71905531
1686 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0530400.1

Sequence Viewer

Length: 474 bp
ATGTCTGCAGTAAGAATGGAGTTAAATAGGGATGAAAAGCCACATGTTTTGGCTGTTGATGATAGCGTTTTGGACCGTAAAATCATCGAGAGGTTACTCACCAATTCTTCATGCAAAGTGACTACAGCGGAGGATGCACATGGAGCACTGGAGCTGTTGGGTTTGGCAGATGGGCAACAGAACTTCTCTAACACCGTTTCCGACGTTAACATGATAATCACTGATTACTGCATGCCAGGAATGACAGGGTACGAGCTACTAAAAAAAATAAAGGAATCACCAACAGCGAAGGAGATACCAGTAGTTGTTGTATCATCTGAGAACATACAGACTCGAATTGAAAAGTGCCTTGAGGAAGGAGCTGAAGAGTTCTTGCTGAAGCCTCTCCGACAGTCGGATGTTAAAAGATTGAGATGCCATCTAACAAAGGATGGAAACCCTAGTGATGAGGGGATGCTCTGTGTGGGAAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

157

Amino Acids

17.45

Weight (kDa)

5.14

Isoelectric Point (pI)

43.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Response_reg PF00072 16 - 134 2.4e-21 Response regulator receiver domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 128
AcuI CTGAAG 2 cut(s) 384, 398
AfaI GTAC 1 cut(s) 251
AfiI CCNNNNNNNGG 1 cut(s) 394
AflIII ACRYGT 1 cut(s) 43
AgsI TTSAA 1 cut(s) 341
AjnI CCWGG 1 cut(s) 235
AluBI AGCT 3 cut(s) 154, 256, 362
AluI AGCT 3 cut(s) 154, 256, 362
Alw21I GWGCWC 1 cut(s) 148
AspS9I GGNCC 1 cut(s) 73
AsuHPI GGTGA 2 cut(s) 91, 270
AvaII GGWCC 1 cut(s) 73
Bbv12I GWGCWC 1 cut(s) 148
BccI CCATC 3 cut(s) 164, 425, 426
BciT130I CCWGG 1 cut(s) 237
BfaI CTAG 1 cut(s) 441
BfmI CTRYAG 2 cut(s) 6, 123
Bme1390I CCNGG 1 cut(s) 237
Bme18I GGWCC 1 cut(s) 73
BmgT120I GGNCC 1 cut(s) 73
BmrFI CCNGG 1 cut(s) 237
BmsI GCATC 3 cut(s) 124, 404, 444
BpmI CTGGAG 1 cut(s) 170
BpuEI CTTGAG 1 cut(s) 371
Bsc4I CCNNNNNNNGG 1 cut(s) 394
Bse1I ACTGG 2 cut(s) 153, 299
BseBI CCWGG 1 cut(s) 237
BseGI GGATG 5 cut(s) 37, 139, 403, 436, 459
BseLI CCNNNNNNNGG 1 cut(s) 394
BseMII CTCAG 1 cut(s) 309
BseNI ACTGG 2 cut(s) 153, 299
BsiHKAI GWGCWC 1 cut(s) 148
BslI CCNNNNNNNGG 1 cut(s) 394
Bsp1286I GDGCHC 1 cut(s) 148
BspACI CCGC 1 cut(s) 128
BspCNI CTCAG 1 cut(s) 310
BspMAI CTGCAG 1 cut(s) 10
BsrI ACTGG 2 cut(s) 153, 299
Bst2UI CCWGG 1 cut(s) 237
Bst4CI ACNGT 3 cut(s) 77, 196, 393
Bst6I CTCTTC 1 cut(s) 360
BstC8I GCNNGC 1 cut(s) 233
BstDEI CTNAG 1 cut(s) 318
BstF5I GGATG 5 cut(s) 37, 139, 403, 436, 459
BstMWI GCNNNNNNNGC 2 cut(s) 134, 143
BstNI CCWGG 1 cut(s) 237
BstNSI RCATGY 2 cut(s) 47, 235
BstSCI CCNGG 1 cut(s) 235
BstSFI CTRYAG 2 cut(s) 6, 123
BtsCI GGATG 5 cut(s) 37, 139, 403, 436, 459
BtsIMutI CAGTG 2 cut(s) 146, 219
Cac8I GCNNGC 1 cut(s) 233
Cfr13I GGNCC 1 cut(s) 73
Csp6I GTAC 1 cut(s) 250
CspCI CAANNNNNGTGG 2 cut(s) 30, 65
CviAII CATG 5 cut(s) 44, 111, 140, 211, 232
CviJI RGCY 6 cut(s) 40, 53, 154, 256, 362, 382
CviKI_1 RGCY 6 cut(s) 40, 53, 154, 256, 362, 382
CviQI GTAC 1 cut(s) 250
DdeI CTNAG 1 cut(s) 318
Eam1104I CTCTTC 1 cut(s) 360
EarI CTCTTC 1 cut(s) 360
Eco47I GGWCC 1 cut(s) 73
Eco57I CTGAAG 2 cut(s) 384, 398
EcoRII CCWGG 1 cut(s) 235
FaeI CATG 5 cut(s) 47, 114, 143, 214, 235
FaiI YATR 6 cut(s) 45, 112, 141, 212, 233, 326
FatI CATG 5 cut(s) 43, 110, 139, 210, 231
FokI GGATG 5 cut(s) 44, 146, 410, 443, 466
FspBI CTAG 1 cut(s) 441
GsuI CTGGAG 1 cut(s) 170
Hin1II CATG 5 cut(s) 47, 114, 143, 214, 235
HincII GTYRAC 1 cut(s) 208
HindII GTYRAC 1 cut(s) 208
HinfI GANTC 2 cut(s) 275, 331
HpaI GTTAAC 1 cut(s) 208
HphI GGTGA 2 cut(s) 91, 270
Hpy166II GTNNAC 1 cut(s) 208
Hpy188I TCNGA 4 cut(s) 202, 319, 389, 397
Hpy188III TCNNGA 1 cut(s) 88
Hpy8I GTNNAC 1 cut(s) 208
Hpy99I CGWCG 1 cut(s) 206
HpyAV CCTTC 2 cut(s) 283, 350
HpyCH4III ACNGT 3 cut(s) 77, 196, 393
HpyCH4IV ACGT 1 cut(s) 204
HpyCH4V TGCA 4 cut(s) 8, 114, 137, 231
HpyF10VI GCNNNNNNNGC 2 cut(s) 134, 143
HpyF3I CTNAG 1 cut(s) 318
HpySE526I ACGT 1 cut(s) 204
Hsp92II CATG 5 cut(s) 47, 114, 143, 214, 235
KspAI GTTAAC 1 cut(s) 208
LmnI GCTCC 3 cut(s) 143, 151, 359
LpnPI CCDG 5 cut(s) 134, 222, 231, 249, 312
LweI GCATC 3 cut(s) 124, 404, 444
MaeI CTAG 1 cut(s) 441
MaeII ACGT 1 cut(s) 204
MaeIII GTNAC 2 cut(s) 93, 118
MboII GAAGA 2 cut(s) 99, 377
MhlI GDGCHC 1 cut(s) 148
MluCI AATT 2 cut(s) 103, 336
MlyI GAGTC 1 cut(s) 325
MmeI TCCRAC 3 cut(s) 225, 375, 412
MnlI CCTC 5 cut(s) 84, 124, 346, 393, 442
MseI TTAA 3 cut(s) 23, 207, 402
MspA1I CMGCKG 1 cut(s) 128
MspR9I CCNGG 1 cut(s) 237
MvaI CCWGG 1 cut(s) 237
MwoI GCNNNNNNNGC 2 cut(s) 134, 143
NlaIII CATG 5 cut(s) 47, 114, 143, 214, 235
NmuCI GTSAC 1 cut(s) 118
NspI RCATGY 2 cut(s) 47, 235
PaeI GCATGC 1 cut(s) 235
PciI ACATGT 1 cut(s) 43
PfeI GAWTC 1 cut(s) 275
PleI GAGTC 1 cut(s) 325
PpsI GAGTC 1 cut(s) 325
PscI ACATGT 1 cut(s) 43
Psp6I CCWGG 1 cut(s) 235
PspGI CCWGG 1 cut(s) 235
PspPI GGNCC 1 cut(s) 73
PstI CTGCAG 1 cut(s) 10
RsaI GTAC 1 cut(s) 251
RsaNI GTAC 1 cut(s) 250
SaqAI TTAA 3 cut(s) 23, 207, 402
Sau96I GGNCC 1 cut(s) 73
SchI GAGTC 1 cut(s) 325
ScrFI CCNGG 1 cut(s) 237
SduI GDGCHC 1 cut(s) 148
SetI ASST 5 cut(s) 95, 156, 207, 258, 364
SfaNI GCATC 3 cut(s) 124, 404, 444
SfcI CTRYAG 2 cut(s) 6, 123
SinI GGWCC 1 cut(s) 73
SmlI CTYRAG 1 cut(s) 350
SmoI CTYRAG 1 cut(s) 350
SphI GCATGC 1 cut(s) 235
Sse9I AATT 2 cut(s) 103, 336
SsiI CCGC 1 cut(s) 128
SspMI CTAG 1 cut(s) 441
StyD4I CCNGG 1 cut(s) 235
TaaI ACNGT 3 cut(s) 77, 196, 393
TaiI ACGT 1 cut(s) 207
TaqI TCGA 2 cut(s) 87, 334
TasI AATT 2 cut(s) 103, 336
TfiI GAWTC 1 cut(s) 275
Tru1I TTAA 3 cut(s) 23, 207, 402
Tru9I TTAA 3 cut(s) 23, 207, 402
TscAI CASTG 2 cut(s) 153, 226
TseFI GTSAC 1 cut(s) 118
Tsp45I GTSAC 1 cut(s) 118
TspDTI ATGAA 2 cut(s) 48, 99
TspRI CASTG 2 cut(s) 153, 226
VpaK11BI GGWCC 1 cut(s) 73
XceI RCATGY 2 cut(s) 47, 235
XspI CTAG 1 cut(s) 441
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.