Basic Information

Type: gene

Biological Identity

Species

rosa_rugosa

Chromosome

GWHBQTZ00000006

Orthogroup ID

OG0007761

Physical Location & Seq

Genomic Coordinates Reverse (-)

22024795 .. 22026509

Gene Structure 1715 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rorug06G0153200.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1230 bp

ATGGAGAAGAGAAAGCTGAAATACAAAGATGAAGCGATATTATTGCAGCACCCACTTGTGCTTTTCCTTATTCTGGTTTTGGGCTTTGTCATAATGGATCCTCTACAAGTAGGCCCGCTAGGCGGCCATGAATTCAAGCCCGTCAAGCACAGCATTGCGCCATACAAGAAAGTCATGGAGAGGTGGCCGAGGGACAATCTGAGCCGGTTGGGACTCGGGAAGTTGGAGTTCGAGGACGAAGTTTTTGGGCCTGAATCTTTGGAGTTTGATGCTTTGGGCCTTGGGCCTTACACCGGGTTAGCCGATGGACGCATCGTGAGATGGATGGGCCCGGATGTGGGTTGGGAGACATTTGCGCTTGTAACAGCAAATTGGTCAGAGCAGGTTTGTGCAAAAGGCAATGAGTCCACCACATACAAGCAATGGAAGCATGAAAAAAGGTGTGGTCGTCCACTAGGGTTAAGATTTGACAAAGTGACTGGAAATCTGTACATTGCGGACGCTTATTATGGACTTCTAGCTGTTGGACCTGAAGGAGGACTAGCAACTCCTTTGTCGACTCAAGACGTAGAAGGCAACCCCATACTCTTTGCAAATGACCTTGATATCCACACCAATGGTTCCATCTTCTTCACAGATACTAGCACGAGATACAACAGAGTGGATCATTTCCTTATACTATTGGAAGGAGAAGCCACCGGTAGGCTCCTGAGATATGACCCTCCCACTAAAACAACTCATGTTGTCTTGCATGGTTTGGCCTTTCCAAATGGCTTACAATTTTCTCAGGACCAAACCTTCCTTCTCTTCACTGAGACCACCAACTGCAGGCTCATGAAATATTGGTTAGAAGGTCCAAAATCTGGAACAGTGGAACTTGTTGCGGACCTTCCAGGATTTCCAGACAATGTAAGAATAAATGAGAAAGGGCAATTCTGGGTAGCAATAGATTGTTGCAGGACTCCAGCACAGGAGGTCCTCTCTAATAATCCATGGTTAAGGAATGTCTACTTTCGCTTGCCAATCCGGATGACCTACTTGGCAAGAATTATGGGAATGAAAATGTACACACTGATCTCGCTATTCAATGACAAGGGAGAGATTTTGGAAGTTCTTGAAGACCGAAAGGGTGAGGTTATGAAACTTGTGAGTGAAGTTAGAGAATCAAAGGGCAAACTCTGGATTGGAACTGTGGCTCATAACCACATTGCTACCGTCCCTTACCCTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005623 GO:0005886 GO:0005911 GO:0009506 GO:0016020 GO:0030054 GO:0044464 GO:0055044 GO:0071944

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Cytochrom_B561 DUF568

Protein Analysis

409

Amino Acids

46.34

Weight (kDa)

6.39

Isoelectric Point (pI)

27.32

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
SGL	PF08450	135 - 316	5.1e-16	SMP-30/Gluconolactonase/LRE-like region
Str_synth	PF03088	199 - 285	4.1e-29	Strictosidine synthase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0007761)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G25290 AT3G25290 AT4G12980
fragaria_vesca	FvH4_2g18000
malus_domestica	MD05G1164000.v1.1 MD10G1153700.v1.1
prunus_persica	Prupe.8G192500_v2.0.a1 Prupe.8G192500_v2.0.a1
pyrus_communis	pycom05g15320 pycom10g13270
rosa_chinensis	RchiOBHm_Chr6g0283371
rosa_laevigata	RLG00000012832
rosa_multiflora	Rmu_sc0003166.1_g000008 Rmu_sc0013038.1_g000001
rosa_roxburghii	Rroxscaffold_7G00185580
rosa_rugosa	Rorug06G0153100 Rorug06G0153200 Rorug06G0153300 Rorug06G0153400
rosa_samantha	Rh6AG265900 Rh6BG268500 Rh6CG268200 Rh6DG261700
rosa_wichuraiana	Rw6G023010

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	373
AccB7I	CCANNNNNTGG	1 cut(s)	863
AccI	GTMKAC	2 cut(s)	557, 1008
AccIII	TCCGGA	1 cut(s)	1026
AciI	CCGC	4 cut(s)	116, 123, 497, 884
AclWI	GGATC	3 cut(s)	92, 105, 672
AcoI	YGGCCR	2 cut(s)	124, 185
AcsI	RAATTY	1 cut(s)	131
AcuI	CTGAAG	1 cut(s)	552
AfaI	GTAC	2 cut(s)	491, 1067
AfiI	CCNNNNNNNGG	9 cut(s)	73, 122, 293, 337, 338, 536, 702, 828, 863
AgeI	ACCGGT	1 cut(s)	698
AgsI	TTSAA	3 cut(s)	136, 1087, 1118
AjnI	CCWGG	1 cut(s)	892
AjuI	GAANNNNNNNTTGG	2 cut(s)	786, 818
AluBI	AGCT	2 cut(s)	16, 521
AluI	AGCT	2 cut(s)	16, 521
Alw26I	GTCTC	2 cut(s)	341, 809
AlwI	GGATC	3 cut(s)	92, 105, 672
Ama87I	CYCGRG	1 cut(s)	215
Aor13HI	TCCGGA	1 cut(s)	1026
AoxI	GGCC	8 cut(s)	112, 124, 185, 248, 277, 284, 328, 759
ApaI	GGGCCC	1 cut(s)	332
ApeKI	GCWGC	1 cut(s)	46
ApoI	RAATTY	1 cut(s)	131
ArsI	GACNNNNNNTTYG	2 cut(s)	227, 259
AsiGI	ACCGGT	1 cut(s)	698
AspLEI	GCGC	2 cut(s)	160, 358
AsuC2I	CCSGG	2 cut(s)	295, 332
AsuHPI	GGTGA	1 cut(s)	1142
AvaI	CYCGRG	1 cut(s)	215
AvaII	GGWCC	5 cut(s)	527, 790, 854, 886, 976
BaeGI	GKGCMC	1 cut(s)	332
BamHI	GGATCC	1 cut(s)	97
BanII	GRGCYC	1 cut(s)	332
BauI	CACGAG	1 cut(s)	646
BbsI	GAAGAC	1 cut(s)	1125
BbvI	GCAGC	1 cut(s)	58
BccI	CCATC	4 cut(s)	299, 315, 319, 632
BcgI	CGANNNNNNTGC	2 cut(s)	25, 59
BciT130I	CCWGG	1 cut(s)	894
BcnI	CCSGG	2 cut(s)	295, 332
BcoDI	GTCTC	2 cut(s)	341, 809
BfaI	CTAG	5 cut(s)	119, 455, 518, 542, 642
BfmI	CTRYAG	1 cut(s)	826
BfuAI	ACCTGC	1 cut(s)	373
BglI	GCCNNNNNGGC	1 cut(s)	120
BisI	GCNGC	2 cut(s)	47, 124
BlsI	GCNGC	2 cut(s)	48, 125
Bme1390I	CCNGG	3 cut(s)	295, 332, 894
Bme18I	GGWCC	5 cut(s)	527, 790, 854, 886, 976
BmeT110I	CYCGRG	1 cut(s)	215
BmiI	GGNNCC	4 cut(s)	99, 330, 622, 707
BmrFI	CCNGG	3 cut(s)	295, 332, 894
BmsI	GCATC	2 cut(s)	259, 321
BpiI	GAAGAC	1 cut(s)	1125
BplI	GAGNNNNNCTC	2 cut(s)	965, 997
BpmI	CTGGAG	1 cut(s)	948
BpuEI	CTTGAG	1 cut(s)	546
BpuMI	CCSGG	2 cut(s)	295, 332
BsaI	GGTCTC	1 cut(s)	809
BsaJI	CCNNGG	3 cut(s)	188, 280, 992
BsaWI	WCCGGW	2 cut(s)	698, 1026
Bsc4I	CCNNNNNNNGG	9 cut(s)	73, 122, 293, 337, 338, 536, 702, 828, 863
Bse118I	RCCGGY	2 cut(s)	204, 698
Bse1I	ACTGG	1 cut(s)	484
Bse3DI	GCAATG	5 cut(s)	153, 406, 428, 492, 1206
BseAI	TCCGGA	1 cut(s)	1026
BseBI	CCWGG	1 cut(s)	894
BseDI	CCNNGG	3 cut(s)	188, 280, 992
BseGI	GGATG	3 cut(s)	330, 340, 1035
BseLI	CCNNNNNNNGG	9 cut(s)	73, 122, 293, 337, 338, 536, 702, 828, 863
BseMI	GCAATG	5 cut(s)	153, 406, 428, 492, 1206
BseMII	CTCAG	4 cut(s)	191, 701, 800, 804
BseNI	ACTGG	1 cut(s)	484
BseSI	GKGCMC	1 cut(s)	332
BseXI	GCAGC	1 cut(s)	58
BshFI	GGCC	8 cut(s)	114, 126, 187, 250, 279, 286, 330, 761
BshTI	ACCGGT	1 cut(s)	698
BsiHKCI	CYCGRG	1 cut(s)	215
BsiSI	CCGG	5 cut(s)	205, 294, 332, 699, 1027
BslFI	GGGAC	3 cut(s)	206, 225, 1202
BslI	CCNNNNNNNGG	9 cut(s)	73, 122, 293, 337, 338, 536, 702, 828, 863
BsmAI	GTCTC	2 cut(s)	341, 809
BsmFI	GGGAC	3 cut(s)	206, 225, 1202
BsnI	GGCC	8 cut(s)	114, 126, 187, 250, 279, 286, 330, 761
Bso31I	GGTCTC	1 cut(s)	809
BsoBI	CYCGRG	1 cut(s)	215
Bsp120I	GGGCCC	1 cut(s)	328
Bsp1286I	GDGCHC	1 cut(s)	332
Bsp13I	TCCGGA	1 cut(s)	1026
Bsp1407I	TGTACA	2 cut(s)	489, 1065
Bsp143I	GATC	3 cut(s)	97, 664, 1074
Bsp19I	CCATGG	1 cut(s)	992
BspACI	CCGC	4 cut(s)	116, 123, 497, 884
BspANI	GGCC	8 cut(s)	114, 126, 187, 250, 279, 286, 330, 761
BspCNI	CTCAG	4 cut(s)	192, 702, 799, 805
BspEI	TCCGGA	1 cut(s)	1026
BspHI	TCATGA	1 cut(s)	834
BspLI	GGNNCC	4 cut(s)	99, 330, 622, 707
BspMAI	CTGCAG	1 cut(s)	830
BspMI	ACCTGC	1 cut(s)	373
BspPI	GGATC	3 cut(s)	92, 105, 672
BspTNI	GGTCTC	1 cut(s)	809
BsrDI	GCAATG	5 cut(s)	153, 406, 428, 492, 1206
BsrFI	RCCGGY	2 cut(s)	204, 698
BsrGI	TGTACA	2 cut(s)	489, 1065
BsrI	ACTGG	1 cut(s)	484
BssAI	RCCGGY	2 cut(s)	204, 698
BssECI	CCNNGG	3 cut(s)	188, 280, 992
BssMI	GATC	3 cut(s)	97, 664, 1074
BssSI	CACGAG	1 cut(s)	646
BssT1I	CCWWGG	2 cut(s)	280, 992
Bst2BI	CACGAG	1 cut(s)	646
Bst2UI	CCWGG	1 cut(s)	894
Bst4CI	ACNGT	3 cut(s)	871, 1192, 1216
Bst6I	CTCTTC	2 cut(s)	2, 812
BstAUI	TGTACA	2 cut(s)	489, 1065
BstC8I	GCNNGC	3 cut(s)	116, 830, 1019
BstDEI	CTNAG	4 cut(s)	200, 710, 786, 813
BstDSI	CCRYGG	1 cut(s)	992
BstENI	CCTNNNNNAGG	1 cut(s)	534
BstF5I	GGATG	3 cut(s)	330, 340, 1035
BstHHI	GCGC	2 cut(s)	160, 358
BstKTI	GATC	3 cut(s)	100, 667, 1077
BstMAI	GTCTC	2 cut(s)	341, 809
BstMBI	GATC	3 cut(s)	97, 664, 1074
BstMWI	GCNNNNNNNGC	3 cut(s)	120, 145, 427
BstNI	CCWGG	1 cut(s)	894
BstSCI	CCNGG	3 cut(s)	293, 330, 892
BstSFI	CTRYAG	1 cut(s)	826
BstSLI	GKGCMC	1 cut(s)	332
BstV1I	GCAGC	1 cut(s)	58
BstV2I	GAAGAC	1 cut(s)	1125
BstX2I	RGATCY	1 cut(s)	97
BstXI	CCANNNNNNTGG	1 cut(s)	617
BstYI	RGATCY	1 cut(s)	97
BsuRI	GGCC	8 cut(s)	114, 126, 187, 250, 279, 286, 330, 761
BtgI	CCRYGG	1 cut(s)	992
BtsCI	GGATG	3 cut(s)	330, 340, 1035
BtsIMutI	CAGTG	3 cut(s)	810, 876, 1070
BveI	ACCTGC	1 cut(s)	373
Cac8I	GCNNGC	3 cut(s)	116, 830, 1019
CciI	TCATGA	1 cut(s)	834
CfoI	GCGC	2 cut(s)	160, 358
Cfr10I	RCCGGY	2 cut(s)	204, 698
CseI	GACGC	2 cut(s)	318, 509
Csp6I	GTAC	2 cut(s)	490, 1066
CspAI	ACCGGT	1 cut(s)	698
CviAII	CATG	7 cut(s)	128, 175, 431, 740, 752, 835, 993
CviQI	GTAC	2 cut(s)	490, 1066
DdeI	CTNAG	4 cut(s)	200, 710, 786, 813
DpnI	GATC	3 cut(s)	99, 666, 1076
DpnII	GATC	3 cut(s)	97, 664, 1074
EaeI	YGGCCR	2 cut(s)	124, 185
Eam1104I	CTCTTC	2 cut(s)	2, 812
EarI	CTCTTC	2 cut(s)	2, 812
Eco130I	CCWWGG	2 cut(s)	280, 992
Eco24I	GRGCYC	1 cut(s)	332
Eco31I	GGTCTC	1 cut(s)	809
Eco32I	GATATC	1 cut(s)	607
Eco47I	GGWCC	5 cut(s)	527, 790, 854, 886, 976
Eco57I	CTGAAG	1 cut(s)	552
Eco88I	CYCGRG	1 cut(s)	215
EcoNI	CCTNNNNNAGG	1 cut(s)	534
EcoO109I	RGGNCCY	1 cut(s)	976
EcoRI	GAATTC	1 cut(s)	131
EcoRII	CCWGG	1 cut(s)	892
EcoRV	GATATC	1 cut(s)	607
EcoT14I	CCWWGG	2 cut(s)	280, 992
EcoT38I	GRGCYC	1 cut(s)	332
ErhI	CCWWGG	2 cut(s)	280, 992
FaeI	CATG	7 cut(s)	131, 178, 434, 743, 755, 838, 996
FaqI	GGGAC	3 cut(s)	206, 225, 1202
FatI	CATG	7 cut(s)	127, 174, 430, 739, 751, 834, 992
FauI	CCCGC	1 cut(s)	123
FblI	GTMKAC	2 cut(s)	557, 1008
Fnu4HI	GCNGC	2 cut(s)	47, 124
FokI	GGATG	3 cut(s)	337, 347, 1042
FriOI	GRGCYC	1 cut(s)	332
Fsp4HI	GCNGC	2 cut(s)	47, 124
FspBI	CTAG	5 cut(s)	119, 455, 518, 542, 642
GlaI	GCGC	2 cut(s)	159, 357
GluI	GCNGC	2 cut(s)	47, 124
GsuI	CTGGAG	1 cut(s)	948
HaeIII	GGCC	8 cut(s)	114, 126, 187, 250, 279, 286, 330, 761
HapII	CCGG	5 cut(s)	205, 294, 332, 699, 1027
HgaI	GACGC	2 cut(s)	318, 509
HhaI	GCGC	2 cut(s)	160, 358
Hin1II	CATG	7 cut(s)	131, 178, 434, 743, 755, 838, 996
Hin6I	GCGC	2 cut(s)	158, 356
HinP1I	GCGC	2 cut(s)	158, 356
HincII	GTYRAC	1 cut(s)	558
HindII	GTYRAC	1 cut(s)	558
HinfI	GANTC	6 cut(s)	213, 254, 404, 559, 961, 1163
HpaII	CCGG	5 cut(s)	205, 294, 332, 699, 1027
HphI	GGTGA	1 cut(s)	1142
Hpy166II	GTNNAC	5 cut(s)	408, 452, 558, 1009, 1068
Hpy188I	TCNGA	2 cut(s)	201, 379
Hpy8I	GTNNAC	5 cut(s)	408, 452, 558, 1009, 1068
HpyAV	CCTTC	7 cut(s)	527, 566, 680, 808, 812, 845, 899
HpyCH4III	ACNGT	3 cut(s)	871, 1192, 1216
HpyCH4IV	ACGT	1 cut(s)	567
HpyCH4V	TGCA	6 cut(s)	46, 392, 593, 751, 828, 957
HpyF10VI	GCNNNNNNNGC	3 cut(s)	120, 145, 427
HpyF3I	CTNAG	4 cut(s)	200, 710, 786, 813
HpySE526I	ACGT	1 cut(s)	567
Hsp92II	CATG	7 cut(s)	131, 178, 434, 743, 755, 838, 996
HspAI	GCGC	2 cut(s)	158, 356
Kpn2I	TCCGGA	1 cut(s)	1026
Kzo9I	GATC	3 cut(s)	97, 664, 1074
LmnI	GCTCC	1 cut(s)	711
Lsp1109I	GCAGC	1 cut(s)	58
LweI	GCATC	2 cut(s)	259, 321
MaeI	CTAG	5 cut(s)	119, 455, 518, 542, 642
MaeII	ACGT	1 cut(s)	567
MaeIII	GTNAC	2 cut(s)	361, 475
MalI	GATC	3 cut(s)	99, 666, 1076
MboI	GATC	3 cut(s)	97, 664, 1074
MboII	GAAGA	5 cut(s)	19, 619, 622, 799, 1130
MflI	RGATCY	1 cut(s)	97
MhlI	GDGCHC	1 cut(s)	332
MluCI	AATT	5 cut(s)	131, 370, 779, 932, 1047
MlyI	GAGTC	4 cut(s)	207, 413, 553, 955
MmeI	TCCRAC	2 cut(s)	204, 505
MnlI	CCTC	9 cut(s)	111, 174, 183, 226, 530, 732, 967, 989, 1126
MroI	TCCGGA	1 cut(s)	1026
MseI	TTAA	3 cut(s)	461, 998, 1228
MslI	CAYNNNNRTG	1 cut(s)	615
MspI	CCGG	5 cut(s)	205, 294, 332, 699, 1027
MspR9I	CCNGG	3 cut(s)	295, 332, 894
MvaI	CCWGG	1 cut(s)	894
MwoI	GCNNNNNNNGC	3 cut(s)	120, 145, 427
NciI	CCSGG	2 cut(s)	295, 332
NcoI	CCATGG	1 cut(s)	992
NdeII	GATC	3 cut(s)	97, 664, 1074
NlaIII	CATG	7 cut(s)	131, 178, 434, 743, 755, 838, 996
NlaIV	GGNNCC	4 cut(s)	99, 330, 622, 707
NmeAIII	GCCGAG	1 cut(s)	213
NmuCI	GTSAC	1 cut(s)	475
PagI	TCATGA	1 cut(s)	834
PfeI	GAWTC	2 cut(s)	254, 1163
PflMI	CCANNNNNTGG	1 cut(s)	863
PfoI	TCCNGGA	1 cut(s)	892
PinAI	ACCGGT	1 cut(s)	698
PkrI	GCNGC	2 cut(s)	48, 125
PleI	GAGTC	4 cut(s)	207, 412, 553, 955
PpsI	GAGTC	4 cut(s)	207, 412, 553, 955
PpuMI	RGGWCCY	1 cut(s)	976
Psp5II	RGGWCCY	1 cut(s)	976
Psp6I	CCWGG	1 cut(s)	892
PspGI	CCWGG	1 cut(s)	892
PspN4I	GGNNCC	4 cut(s)	99, 330, 622, 707
PspOMI	GGGCCC	1 cut(s)	328
PspPPI	RGGWCCY	1 cut(s)	976
PstI	CTGCAG	1 cut(s)	830
PsuI	RGATCY	1 cut(s)	97
RsaI	GTAC	2 cut(s)	491, 1067
RsaNI	GTAC	2 cut(s)	490, 1066
RseI	CAYNNNNRTG	1 cut(s)	615
SalI	GTCGAC	1 cut(s)	556
SaqAI	TTAA	3 cut(s)	461, 998, 1228
SatI	GCNGC	2 cut(s)	47, 124
Sau3AI	GATC	3 cut(s)	97, 664, 1074
SchI	GAGTC	4 cut(s)	207, 413, 553, 955
ScrFI	CCNGG	3 cut(s)	295, 332, 894
SduI	GDGCHC	1 cut(s)	332
SfaNI	GCATC	2 cut(s)	259, 321
SfcI	CTRYAG	1 cut(s)	826
SinI	GGWCC	5 cut(s)	527, 790, 854, 886, 976
SmiMI	CAYNNNNRTG	1 cut(s)	615
SmlI	CTYRAG	1 cut(s)	561
SmoI	CTYRAG	1 cut(s)	561
Sse9I	AATT	5 cut(s)	131, 370, 779, 932, 1047
SsiI	CCGC	4 cut(s)	116, 123, 497, 884
SspI	AATATT	1 cut(s)	842
SspMI	CTAG	5 cut(s)	119, 455, 518, 542, 642
StyD4I	CCNGG	3 cut(s)	293, 330, 892
StyI	CCWWGG	2 cut(s)	280, 992
TaaI	ACNGT	3 cut(s)	871, 1192, 1216
TaiI	ACGT	1 cut(s)	570
TaqI	TCGA	2 cut(s)	231, 557
TaqII	GACCGA	1 cut(s)	1137
TasI	AATT	5 cut(s)	131, 370, 779, 932, 1047
TatI	WGTACW	2 cut(s)	489, 1065
TauI	GCSGC	1 cut(s)	126
TfiI	GAWTC	2 cut(s)	254, 1163
Tru1I	TTAA	3 cut(s)	461, 998, 1228
Tru9I	TTAA	3 cut(s)	461, 998, 1228
TscAI	CASTG	3 cut(s)	817, 876, 1077
TseFI	GTSAC	1 cut(s)	475
TseI	GCWGC	1 cut(s)	46
Tsp45I	GTSAC	1 cut(s)	475
TspDTI	ATGAA	6 cut(s)	45, 144, 447, 851, 1073, 1154
TspRI	CASTG	3 cut(s)	817, 876, 1077
Van91I	CCANNNNNTGG	1 cut(s)	863
VpaK11BI	GGWCC	5 cut(s)	527, 790, 854, 886, 976
XagI	CCTNNNNNAGG	1 cut(s)	534
XapI	RAATTY	1 cut(s)	131
XmiI	GTMKAC	2 cut(s)	557, 1008
XspI	CTAG	5 cut(s)	119, 455, 518, 542, 642

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.