Rw6G023010

Auxin-induced in root cultures protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0007761
Physical Location & Seq
Reverse (-)
45210270 .. 45213789
3520 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G023010.1

Sequence Viewer

Length: 804 bp
ATGGCTTCTTCTTCATCTTCTTCTTGTGCTAACCAGAACCTGGCGACGCTAATCTCACTGACTGTGATTCTGCTAGTCTCACCCGCGTATTCCCAGAGCTGCTCCTCCCAGTCCTTCGCCAACAAAATGCTCTACACCCAATGCGCCGACCTCCCAACCCTCAGCTCTGCCCTCCACTGGTACTACGACTCCGCCAACTCCACTCTCGCCATCGCCTTCATCGCGCCGCCGGCAAAGCCCGCCGGATGGACCTCCTGGGCCATCAACCCCACCGGCACCGGCATGGCCGGGGCCCAAACGCTCCTCGCCTACAAAACCTCCTCCGGCGCCATGGCCGTCAAGACCTTCAACATCAGCTCCTACAGCTCCATCGTACCCGGAAAGCTCGCCTTCGACGTCTGGGACACGGCCGCTGAGTCCTCCGGCGGGGTCTTCCGGTTGTTTGCCAAGGTGAAGGTGAAGACTGGGCAGGTGAACCACGTCTGGCAGGTGGGGCCGTCCGTCGTAGATGGGTTTCCGGCGAAGCATGCTTTTGATGCACCCAATCTTCAGGCGAAAGGGACCCTCACCTTGACCGGAACTAATGCCGGTGTCACCGGGGCCGGAGCCGATCCCAAGTTGAAGGACAGGAATATTCACGGAATTCTGAATGCTGTGAGTTGGGGGTTTTTGTTTCCACTGGGGATCATTATAGCAAGGTACATCAGGACTTTTCCGTCTGCGGATCCTGCATGGTTTTATCTCATATATCCTGCCAAATATCTGCTTATGCCATCGGCGTCGCCGGCTGGGCCACCGGACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0005911 GO:0009506 GO:0016020 GO:0030054 GO:0044464 GO:0055044 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cytochrom_B561 DUF568

Protein Analysis

267

Amino Acids

28.11

Weight (kDa)

9.2

Isoelectric Point (pI)

45.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF568 PF04526 95 - 191 1.9e-27 Protein of unknown function (DUF568)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 460, 478
AasI GACNNNNNNGTC 1 cut(s) 715
AatII GACGTC 1 cut(s) 399
Acc36I ACCTGC 2 cut(s) 460, 478
AccB1I GGYRCC 2 cut(s) 275, 326
AccB7I CCANNNNNTGG 1 cut(s) 40
AccII CGCG 2 cut(s) 86, 224
AciI CCGC 7 cut(s) 84, 192, 227, 240, 411, 426, 722
AclWI GGATC 4 cut(s) 605, 692, 719, 732
AcoI YGGCCR 3 cut(s) 285, 333, 408
AcsI RAATTY 1 cut(s) 642
AcuI CTGAAG 1 cut(s) 533
AcyI GRCGYC 3 cut(s) 327, 396, 779
AfaI GTAC 3 cut(s) 182, 375, 701
AfiI CCNNNNNNNGG 4 cut(s) 40, 177, 246, 426
AgsI TTSAA 2 cut(s) 349, 622
AjiI CACGTC 1 cut(s) 481
AjnI CCWGG 2 cut(s) 39, 254
AluBI AGCT 5 cut(s) 99, 165, 357, 366, 385
AluI AGCT 5 cut(s) 99, 165, 357, 366, 385
Alw26I GTCTC 1 cut(s) 82
AlwI GGATC 4 cut(s) 605, 692, 719, 732
AlwNI CAGNNNCTG 1 cut(s) 40
AoxI GGCC 8 cut(s) 258, 285, 291, 333, 408, 494, 600, 791
ApaI GGGCCC 1 cut(s) 295
ApeKI GCWGC 1 cut(s) 99
ApoI RAATTY 1 cut(s) 642
AspLEI GCGC 3 cut(s) 146, 226, 329
AspS9I GGNCC 8 cut(s) 249, 258, 291, 292, 494, 561, 600, 791
AsuC2I CCSGG 3 cut(s) 289, 378, 598
AsuHPI GGTGA 6 cut(s) 72, 463, 469, 484, 559, 586
AvaII GGWCC 2 cut(s) 249, 561
BaeGI GKGCMC 1 cut(s) 295
BamHI GGATCC 1 cut(s) 724
BanI GGYRCC 2 cut(s) 275, 326
BanII GRGCYC 1 cut(s) 295
BbsI GAAGAC 2 cut(s) 424, 467
BbvCI CCTCAGC 1 cut(s) 161
BbvI GCAGC 1 cut(s) 86
BccI CCATC 6 cut(s) 218, 240, 269, 377, 503, 781
BceAI ACGGC 3 cut(s) 320, 423, 481
BciT130I CCWGG 2 cut(s) 41, 256
BcnI CCSGG 3 cut(s) 289, 378, 598
BcoDI GTCTC 1 cut(s) 82
BfaI CTAG 1 cut(s) 74
BfmI CTRYAG 1 cut(s) 361
BfoI RGCGCY 1 cut(s) 330
BfuAI ACCTGC 2 cut(s) 460, 478
BglI GCCNNNNNGGC 1 cut(s) 790
BisI GCNGC 3 cut(s) 100, 227, 411
BlsI GCNGC 3 cut(s) 101, 228, 412
Bme1390I CCNGG 5 cut(s) 41, 256, 289, 378, 598
Bme18I GGWCC 2 cut(s) 249, 561
BmgBI CACGTC 1 cut(s) 481
BmgT120I GGNCC 8 cut(s) 249, 258, 291, 292, 494, 561, 600, 791
BmrFI CCNGG 5 cut(s) 41, 256, 289, 378, 598
BmrI ACTGGG 3 cut(s) 103, 474, 689
BmsI GCATC 1 cut(s) 526
BmuI ACTGGG 3 cut(s) 103, 474, 689
BpiI GAAGAC 2 cut(s) 424, 467
Bpu10I CCTNAGC 1 cut(s) 161
BpuMI CCSGG 3 cut(s) 289, 378, 598
BsaHI GRCGYC 3 cut(s) 327, 396, 779
BsaJI CCNNGG 5 cut(s) 255, 288, 330, 447, 597
BsaWI WCCGGW 3 cut(s) 435, 575, 796
BsaXI ACNNNNNCTCC 6 cut(s) 173, 203, 302, 332, 341, 371
Bsc4I CCNNNNNNNGG 4 cut(s) 40, 177, 246, 426
Bse118I RCCGGY 5 cut(s) 229, 272, 278, 587, 784
Bse1I ACTGG 4 cut(s) 109, 182, 469, 684
BseBI CCWGG 2 cut(s) 41, 256
BseDI CCNNGG 5 cut(s) 255, 288, 330, 447, 597
BseGI GGATG 1 cut(s) 251
BseLI CCNNNNNNNGG 4 cut(s) 40, 177, 246, 426
BseMII CTCAG 2 cut(s) 175, 405
BseNI ACTGG 4 cut(s) 109, 182, 469, 684
BseRI GAGGAG 3 cut(s) 94, 293, 310
BseSI GKGCMC 1 cut(s) 295
BseX3I CGGCCG 1 cut(s) 408
BseXI GCAGC 1 cut(s) 86
BseYI CCCAGC 1 cut(s) 788
Bsh1236I CGCG 2 cut(s) 86, 224
Bsh1285I CGRYCG 1 cut(s) 411
BshFI GGCC 8 cut(s) 260, 287, 293, 335, 410, 496, 602, 793
BshNI GGYRCC 2 cut(s) 275, 326
BsiEI CGRYCG 1 cut(s) 411
BslFI GGGAC 2 cut(s) 416, 574
BslI CCNNNNNNNGG 4 cut(s) 40, 177, 246, 426
BsmAI GTCTC 1 cut(s) 82
BsmFI GGGAC 2 cut(s) 416, 574
BsmI GAATGC 1 cut(s) 655
BsnI GGCC 8 cut(s) 260, 287, 293, 335, 410, 496, 602, 793
Bsp120I GGGCCC 1 cut(s) 291
Bsp1286I GDGCHC 1 cut(s) 295
Bsp143I GATC 3 cut(s) 610, 684, 724
Bsp19I CCATGG 1 cut(s) 330
BspACI CCGC 7 cut(s) 84, 192, 227, 240, 411, 426, 722
BspANI GGCC 8 cut(s) 260, 287, 293, 335, 410, 496, 602, 793
BspCNI CTCAG 2 cut(s) 174, 406
BspFNI CGCG 2 cut(s) 86, 224
BspMI ACCTGC 2 cut(s) 460, 478
BspPI GGATC 4 cut(s) 605, 692, 719, 732
BspT107I GGYRCC 2 cut(s) 275, 326
BsrFI RCCGGY 5 cut(s) 229, 272, 278, 587, 784
BsrI ACTGG 4 cut(s) 109, 182, 469, 684
BssAI RCCGGY 5 cut(s) 229, 272, 278, 587, 784
BssECI CCNNGG 5 cut(s) 255, 288, 330, 447, 597
BssMI GATC 3 cut(s) 610, 684, 724
BssNI GRCGYC 3 cut(s) 327, 396, 779
BssT1I CCWWGG 2 cut(s) 330, 447
Bst2UI CCWGG 2 cut(s) 41, 256
Bst4CI ACNGT 1 cut(s) 64
BstACI GRCGYC 3 cut(s) 327, 396, 779
BstC8I GCNNGC 5 cut(s) 231, 240, 387, 528, 786
BstDEI CTNAG 2 cut(s) 161, 414
BstDSI CCRYGG 1 cut(s) 330
BstF5I GGATG 1 cut(s) 251
BstFNI CGCG 2 cut(s) 86, 224
BstH2I RGCGCY 1 cut(s) 330
BstHHI GCGC 3 cut(s) 146, 226, 329
BstKTI GATC 3 cut(s) 613, 687, 727
BstMAI GTCTC 1 cut(s) 82
BstMBI GATC 3 cut(s) 610, 684, 724
BstMCI CGRYCG 1 cut(s) 411
BstNI CCWGG 2 cut(s) 41, 256
BstNSI RCATGY 1 cut(s) 530
BstSCI CCNGG 5 cut(s) 39, 254, 287, 376, 596
BstSFI CTRYAG 1 cut(s) 361
BstSLI GKGCMC 1 cut(s) 295
BstUI CGCG 2 cut(s) 86, 224
BstV1I GCAGC 1 cut(s) 86
BstV2I GAAGAC 2 cut(s) 424, 467
BstX2I RGATCY 1 cut(s) 724
BstYI RGATCY 1 cut(s) 724
BstZI CGGCCG 1 cut(s) 408
BsuRI GGCC 8 cut(s) 260, 287, 293, 335, 410, 496, 602, 793
BtgI CCRYGG 1 cut(s) 330
BtgZI GCGATG 2 cut(s) 196, 205
BtrI CACGTC 1 cut(s) 481
BtsCI GGATG 1 cut(s) 251
BtsIMutI CAGTG 3 cut(s) 56, 175, 677
BveI ACCTGC 2 cut(s) 460, 478
Cac8I GCNNGC 5 cut(s) 231, 240, 387, 528, 786
CaiI CAGNNNCTG 1 cut(s) 40
CfoI GCGC 3 cut(s) 146, 226, 329
Cfr10I RCCGGY 5 cut(s) 229, 272, 278, 587, 784
Cfr13I GGNCC 8 cut(s) 249, 258, 291, 292, 494, 561, 600, 791
CseI GACGC 2 cut(s) 55, 768
Csp6I GTAC 3 cut(s) 181, 374, 700
CviAII CATG 4 cut(s) 283, 331, 527, 732
CviQI GTAC 3 cut(s) 181, 374, 700
DdeI CTNAG 2 cut(s) 161, 414
DinI GGCGCC 1 cut(s) 328
DpnI GATC 3 cut(s) 612, 686, 726
DpnII GATC 3 cut(s) 610, 684, 724
DrdI GACNNNNNNGTC 1 cut(s) 715
DseDI GACNNNNNNGTC 1 cut(s) 715
EaeI YGGCCR 3 cut(s) 285, 333, 408
EagI CGGCCG 1 cut(s) 408
EciI GGCGGA 1 cut(s) 181
EclXI CGGCCG 1 cut(s) 408
Eco130I CCWWGG 2 cut(s) 330, 447
Eco24I GRGCYC 1 cut(s) 295
Eco47I GGWCC 2 cut(s) 249, 561
Eco52I CGGCCG 1 cut(s) 408
Eco57I CTGAAG 1 cut(s) 533
EcoO109I RGGNCCY 2 cut(s) 291, 561
EcoRI GAATTC 1 cut(s) 642
EcoRII CCWGG 2 cut(s) 39, 254
EcoT14I CCWWGG 2 cut(s) 330, 447
EcoT38I GRGCYC 1 cut(s) 295
EgeI GGCGCC 1 cut(s) 328
EheI GGCGCC 1 cut(s) 328
ErhI CCWWGG 2 cut(s) 330, 447
FaeI CATG 4 cut(s) 286, 334, 530, 735
FaiI YATR 8 cut(s) 284, 332, 528, 692, 733, 746, 748, 770
FalI AAGNNNNNCTT 2 cut(s) 374, 406
FaqI GGGAC 2 cut(s) 416, 574
FatI CATG 4 cut(s) 282, 330, 526, 731
FauI CCCGC 3 cut(s) 91, 247, 419
Fnu4HI GCNGC 3 cut(s) 100, 227, 411
FokI GGATG 1 cut(s) 258
FriOI GRGCYC 1 cut(s) 295
Fsp4HI GCNGC 3 cut(s) 100, 227, 411
FspBI CTAG 1 cut(s) 74
GlaI GCGC 3 cut(s) 145, 225, 328
GluI GCNGC 3 cut(s) 100, 227, 411
GsaI CCCAGC 1 cut(s) 792
HaeII RGCGCY 1 cut(s) 330
HaeIII GGCC 8 cut(s) 260, 287, 293, 335, 410, 496, 602, 793
HgaI GACGC 2 cut(s) 55, 768
HhaI GCGC 3 cut(s) 146, 226, 329
Hin1I GRCGYC 3 cut(s) 327, 396, 779
Hin1II CATG 4 cut(s) 286, 334, 530, 735
Hin6I GCGC 3 cut(s) 144, 224, 327
HinP1I GCGC 3 cut(s) 144, 224, 327
HinfI GANTC 3 cut(s) 67, 188, 416
HphI GGTGA 6 cut(s) 72, 463, 469, 484, 559, 586
Hpy166II GTNNAC 1 cut(s) 475
Hpy188I TCNGA 1 cut(s) 648
Hpy188III TCNNGA 2 cut(s) 340, 706
Hpy8I GTNNAC 1 cut(s) 475
Hpy99I CGWCG 4 cut(s) 49, 398, 506, 784
HpyAV CCTTC 6 cut(s) 124, 226, 355, 400, 448, 616
HpyCH4III ACNGT 1 cut(s) 64
HpyCH4IV ACGT 2 cut(s) 396, 480
HpyCH4V TGCA 2 cut(s) 539, 731
HpyF3I CTNAG 2 cut(s) 161, 414
HpySE526I ACGT 2 cut(s) 396, 480
Hsp92I GRCGYC 3 cut(s) 327, 396, 779
Hsp92II CATG 4 cut(s) 286, 334, 530, 735
HspAI GCGC 3 cut(s) 144, 224, 327
KasI GGCGCC 1 cut(s) 326
KflI GGGWCCC 1 cut(s) 561
KroI GCCGGC 2 cut(s) 229, 784
KroNI GCCGGC 2 cut(s) 231, 786
Kzo9I GATC 3 cut(s) 610, 684, 724
LmnI GCTCC 5 cut(s) 107, 306, 362, 371, 605
Lsp1109I GCAGC 1 cut(s) 86
LweI GCATC 1 cut(s) 526
MaeI CTAG 1 cut(s) 74
MaeII ACGT 2 cut(s) 396, 480
MaeIII GTNAC 1 cut(s) 592
MalI GATC 3 cut(s) 612, 686, 726
MboI GATC 3 cut(s) 610, 684, 724
MboII GAAGA 6 cut(s) 3, 9, 12, 424, 472, 539
MflI RGATCY 1 cut(s) 724
MhlI GDGCHC 1 cut(s) 295
MluCI AATT 1 cut(s) 642
Mly113I GGCGCC 1 cut(s) 327
MlyI GAGTC 2 cut(s) 182, 425
MroNI GCCGGC 2 cut(s) 229, 784
MslI CAYNNNNRTG 1 cut(s) 281
MspA1I CMGCKG 1 cut(s) 413
MspR9I CCNGG 5 cut(s) 41, 256, 289, 378, 598
Mva1269I GAATGC 1 cut(s) 655
MvaI CCWGG 2 cut(s) 41, 256
MvnI CGCG 2 cut(s) 86, 224
NaeI GCCGGC 2 cut(s) 231, 786
NarI GGCGCC 1 cut(s) 327
NciI CCSGG 3 cut(s) 289, 378, 598
NcoI CCATGG 1 cut(s) 330
NdeII GATC 3 cut(s) 610, 684, 724
NgoMIV GCCGGC 2 cut(s) 229, 784
NlaIII CATG 4 cut(s) 286, 334, 530, 735
NmuCI GTSAC 1 cut(s) 592
NspI RCATGY 1 cut(s) 530
PaeI GCATGC 1 cut(s) 530
PaqCI CACCTGC 2 cut(s) 460, 478
PcsI WCGNNNNNNNCGW 1 cut(s) 393
PctI GAATGC 1 cut(s) 655
PdiI GCCGGC 2 cut(s) 231, 786
PfeI GAWTC 1 cut(s) 67
PflMI CCANNNNNTGG 1 cut(s) 40
PkrI GCNGC 3 cut(s) 101, 228, 412
PleI GAGTC 2 cut(s) 182, 424
PluTI GGCGCC 1 cut(s) 330
PpsI GAGTC 2 cut(s) 182, 424
PpuMI RGGWCCY 1 cut(s) 561
Psp5II RGGWCCY 1 cut(s) 561
Psp6I CCWGG 2 cut(s) 39, 254
PspFI CCCAGC 1 cut(s) 788
PspGI CCWGG 2 cut(s) 39, 254
PspOMI GGGCCC 1 cut(s) 291
PspPI GGNCC 8 cut(s) 249, 258, 291, 292, 494, 561, 600, 791
PspPPI RGGWCCY 1 cut(s) 561
PstNI CAGNNNCTG 1 cut(s) 40
PsuI RGATCY 1 cut(s) 724
RsaI GTAC 3 cut(s) 182, 375, 701
RsaNI GTAC 3 cut(s) 181, 374, 700
RseI CAYNNNNRTG 1 cut(s) 281
SatI GCNGC 3 cut(s) 100, 227, 411
Sau3AI GATC 3 cut(s) 610, 684, 724
Sau96I GGNCC 8 cut(s) 249, 258, 291, 292, 494, 561, 600, 791
SchI GAGTC 2 cut(s) 182, 425
ScrFI CCNGG 5 cut(s) 41, 256, 289, 378, 598
SduI GDGCHC 1 cut(s) 295
SfaNI GCATC 1 cut(s) 526
SfcI CTRYAG 1 cut(s) 361
SfoI GGCGCC 1 cut(s) 328
SinI GGWCC 2 cut(s) 249, 561
SmiMI CAYNNNNRTG 1 cut(s) 281
SphI GCATGC 1 cut(s) 530
Sse9I AATT 1 cut(s) 642
SsiI CCGC 7 cut(s) 84, 192, 227, 240, 411, 426, 722
SspDI GGCGCC 1 cut(s) 326
SspI AATATT 1 cut(s) 634
SspMI CTAG 1 cut(s) 74
StyD4I CCNGG 5 cut(s) 39, 254, 287, 376, 596
StyI CCWWGG 2 cut(s) 330, 447
TaaI ACNGT 1 cut(s) 64
TaiI ACGT 2 cut(s) 399, 483
TaqI TCGA 1 cut(s) 393
TasI AATT 1 cut(s) 642
TauI GCSGC 2 cut(s) 229, 413
TfiI GAWTC 1 cut(s) 67
TscAI CASTG 3 cut(s) 63, 182, 684
TseFI GTSAC 1 cut(s) 592
TseI GCWGC 1 cut(s) 99
Tsp45I GTSAC 1 cut(s) 592
TspDTI ATGAA 1 cut(s) 208
TspGWI ACGGA 3 cut(s) 490, 654, 705
TspRI CASTG 3 cut(s) 63, 182, 684
Van91I CCANNNNNTGG 1 cut(s) 40
VpaK11BI GGWCC 2 cut(s) 249, 561
XapI RAATTY 1 cut(s) 642
XceI RCATGY 1 cut(s) 530
XspI CTAG 1 cut(s) 74
ZraI GACGTC 1 cut(s) 397
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.