Rorug06G0228700

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0005173
Physical Location & Seq
Forward (+)
36764577 .. 36767205
2629 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0228700.1

Sequence Viewer

Length: 846 bp
ATGACGAAGGAAGTGAGCGAGGAACCCCAAGTCCACCACCACGGCAAGGACTACGTCGACCCACCGCCTGCTCCGCTCTTCGACATGGACGAGCTTAAGCGCTGGTCCTTCTACAGAGCTCTCATAGCCGAGTTCATCGCCACCCTCCTCTTCCTCTACGTCACCGTCGCCACAGTCATCGGCCACAAGCACCAGACCGGCCCCTGCGACGGCGTCGGCCTTCTCGGCATCGCCTGGGCCTTTGGTGGCATGATCTTTGTCCTCGTTTACTGCACCGCCGGTATTTCTGGTGGTCACATTAACCCGGCGGTGACTTTCGGGCTTTTCTTGGCTCGTAAGGTCTCTCTGATCCGGGCCGTGGCTTACATGGTCGTCCAGTGCTTGGGAGCTATCGCCGGTGTCGCGCTCGTCAAGGCCTTCCAGAGTAGCTACTTCAAGACCCTCGGCGGCGGCACCAACTCTGTCTCTGCGGGCTACAGCAAGGGCACAGCTCTCGGCGCTGAGATCATCGGCACCTTTGTGCTTGTCTACACCGTCTTCTCAGCCACCGACCCCAAGAGGAGCGCGCGTGACTCTCACGTGCCTGTGTTGGCTCCTTTGCCTATTGGGTTTGCTGTGTTTGTTGTGCACTTGGCTACCATCCCCATCACCGGAACTGGCATCAACCCTGCTCGGAGCTTCGGTGCTGCCGTCATTTACAACAACGAGAAAGTCTGGGATGACCATTGGATCTTCTGGGTCGGACCCTTCCTCGGAGCTCTTTGTGCAGCCGCGTACCACCAGTACATCCTGAGAGCCGCCGCCATCAAAGCTTTGGGTTCATTCCGGAGCCACCCCTCCAACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

281

Amino Acids

30.11

Weight (kDa)

8.83

Isoelectric Point (pI)

33.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MIP PF00230 31 - 260 5.2e-82 Major intrinsic protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 452, 512
AccB7I CCANNNNNTGG 1 cut(s) 382
AccBSI CCGCTC 1 cut(s) 76
AccI GTMKAC 2 cut(s) 57, 528
AccII CGCG 4 cut(s) 404, 566, 568, 773
AccIII TCCGGA 1 cut(s) 825
AclWI GGATC 2 cut(s) 343, 737
AcoI YGGCCR 1 cut(s) 181
AcvI CACGTG 1 cut(s) 580
AcyI GRCGYC 1 cut(s) 213
AfaI GTAC 2 cut(s) 776, 785
AfeI AGCGCT 1 cut(s) 101
AfiI CCNNNNNNNGG 6 cut(s) 46, 209, 358, 382, 650, 752
AflII CTTAAG 1 cut(s) 95
AgsI TTSAA 1 cut(s) 436
AjnI CCWGG 1 cut(s) 233
AleI CACNNNNGTG 1 cut(s) 518
AloI GAACNNNNNNTCC 2 cut(s) 15, 47
AluBI AGCT 8 cut(s) 94, 119, 389, 429, 491, 678, 758, 812
AluI AGCT 8 cut(s) 94, 119, 389, 429, 491, 678, 758, 812
Alw21I GWGCWC 3 cut(s) 121, 630, 760
Alw26I GTCTC 2 cut(s) 346, 469
Alw44I GTGCAC 1 cut(s) 626
AlwI GGATC 2 cut(s) 343, 737
Aor13HI TCCGGA 1 cut(s) 825
Aor51HI AGCGCT 1 cut(s) 101
AoxI GGCC 6 cut(s) 181, 199, 217, 237, 354, 414
ApaLI GTGCAC 1 cut(s) 626
ApeKI GCWGC 2 cut(s) 686, 767
AspLEI GCGC 5 cut(s) 102, 406, 500, 566, 568
AspS9I GGNCC 5 cut(s) 105, 200, 237, 354, 743
AsuC2I CCSGG 2 cut(s) 305, 353
AsuHPI GGTGA 3 cut(s) 154, 322, 640
AvaII GGWCC 2 cut(s) 105, 743
BaeGI GKGCMC 2 cut(s) 488, 630
BanI GGYRCC 2 cut(s) 452, 512
BanII GRGCYC 2 cut(s) 121, 760
BbrPI CACGTG 1 cut(s) 580
BbsI GAAGAC 1 cut(s) 529
Bbv12I GWGCWC 3 cut(s) 121, 630, 760
BbvI GCAGC 2 cut(s) 673, 779
BccI CCATC 3 cut(s) 647, 653, 812
BceAI ACGGC 4 cut(s) 58, 226, 341, 674
BcgI CGANNNNNNTGC 2 cut(s) 475, 509
BciT130I CCWGG 1 cut(s) 235
BcnI CCSGG 2 cut(s) 305, 353
BcoDI GTCTC 2 cut(s) 346, 469
BfmI CTRYAG 2 cut(s) 112, 475
BfoI RGCGCY 2 cut(s) 103, 501
BfrI CTTAAG 1 cut(s) 95
BglI GCCNNNNNGGC 1 cut(s) 225
BisI GCNGC 7 cut(s) 448, 451, 687, 768, 771, 798, 801
BlsI GCNGC 7 cut(s) 449, 452, 688, 769, 772, 799, 802
Bme1390I CCNGG 3 cut(s) 235, 305, 353
Bme18I GGWCC 2 cut(s) 105, 743
BmgT120I GGNCC 5 cut(s) 105, 200, 237, 354, 743
BmiI GGNNCC 7 cut(s) 24, 202, 454, 514, 594, 745, 830
BmrFI CCNGG 3 cut(s) 235, 305, 353
BmsI GCATC 2 cut(s) 237, 669
BpiI GAAGAC 1 cut(s) 529
BpuMI CCSGG 2 cut(s) 305, 353
BsaAI YACGTR 1 cut(s) 580
BsaHI GRCGYC 1 cut(s) 213
BsaI GGTCTC 1 cut(s) 346
BsaJI CCNNGG 5 cut(s) 40, 234, 357, 442, 751
BsaWI WCCGGW 2 cut(s) 650, 825
BsaXI ACNNNNNCTCC 4 cut(s) 553, 583, 667, 697
Bsc4I CCNNNNNNNGG 6 cut(s) 46, 209, 358, 382, 650, 752
Bse118I RCCGGY 3 cut(s) 197, 278, 395
Bse1I ACTGG 3 cut(s) 376, 661, 781
BseAI TCCGGA 1 cut(s) 825
BseBI CCWGG 1 cut(s) 235
BseDI CCNNGG 5 cut(s) 40, 234, 357, 442, 751
BseGI GGATG 3 cut(s) 639, 724, 786
BseLI CCNNNNNNNGG 6 cut(s) 46, 209, 358, 382, 650, 752
BseMII CTCAG 3 cut(s) 492, 555, 782
BseNI ACTGG 3 cut(s) 376, 661, 781
BsePI GCGCGC 1 cut(s) 564
BseRI GAGGAG 2 cut(s) 137, 574
BseSI GKGCMC 2 cut(s) 488, 630
BseXI GCAGC 2 cut(s) 673, 779
BsgI GTGCAG 2 cut(s) 256, 786
Bsh1236I CGCG 4 cut(s) 404, 566, 568, 773
BshFI GGCC 6 cut(s) 183, 201, 219, 239, 356, 416
BshNI GGYRCC 2 cut(s) 452, 512
BsiHKAI GWGCWC 3 cut(s) 121, 630, 760
BsiSI CCGG 7 cut(s) 198, 279, 305, 352, 396, 651, 826
BslI CCNNNNNNNGG 6 cut(s) 46, 209, 358, 382, 650, 752
BsmAI GTCTC 2 cut(s) 346, 469
BsnI GGCC 6 cut(s) 183, 201, 219, 239, 356, 416
Bso31I GGTCTC 1 cut(s) 346
Bsp1286I GDGCHC 4 cut(s) 121, 488, 630, 760
Bsp13I TCCGGA 1 cut(s) 825
Bsp143I GATC 4 cut(s) 252, 348, 504, 729
BspANI GGCC 6 cut(s) 183, 201, 219, 239, 356, 416
BspCNI CTCAG 3 cut(s) 493, 554, 783
BspEI TCCGGA 1 cut(s) 825
BspFNI CGCG 4 cut(s) 404, 566, 568, 773
BspLI GGNNCC 7 cut(s) 24, 202, 454, 514, 594, 745, 830
BspPI GGATC 2 cut(s) 343, 737
BspQI GCTCTTC 1 cut(s) 83
BspT107I GGYRCC 2 cut(s) 452, 512
BspTI CTTAAG 1 cut(s) 95
BspTNI GGTCTC 1 cut(s) 346
BsrBI CCGCTC 1 cut(s) 76
BsrFI RCCGGY 3 cut(s) 197, 278, 395
BsrI ACTGG 3 cut(s) 376, 661, 781
BssAI RCCGGY 3 cut(s) 197, 278, 395
BssECI CCNNGG 5 cut(s) 40, 234, 357, 442, 751
BssHII GCGCGC 1 cut(s) 564
BssMI GATC 4 cut(s) 252, 348, 504, 729
BssNI GRCGYC 1 cut(s) 213
Bst2UI CCWGG 1 cut(s) 235
Bst4CI ACNGT 3 cut(s) 166, 175, 535
Bst6I CTCTTC 2 cut(s) 83, 155
BstACI GRCGYC 1 cut(s) 213
BstAFI CTTAAG 1 cut(s) 95
BstBAI YACGTR 1 cut(s) 580
BstC8I GCNNGC 3 cut(s) 69, 472, 566
BstDEI CTNAG 3 cut(s) 501, 541, 791
BstDSI CCRYGG 2 cut(s) 40, 357
BstF5I GGATG 3 cut(s) 639, 724, 786
BstFNI CGCG 4 cut(s) 404, 566, 568, 773
BstH2I RGCGCY 2 cut(s) 103, 501
BstHHI GCGC 5 cut(s) 102, 406, 500, 566, 568
BstKTI GATC 4 cut(s) 255, 351, 507, 732
BstMAI GTCTC 2 cut(s) 346, 469
BstMBI GATC 4 cut(s) 252, 348, 504, 729
BstMWI GCNNNNNNNGC 7 cut(s) 73, 125, 225, 401, 497, 764, 809
BstNI CCWGG 1 cut(s) 235
BstSCI CCNGG 3 cut(s) 233, 303, 351
BstSFI CTRYAG 2 cut(s) 112, 475
BstSLI GKGCMC 2 cut(s) 488, 630
BstUI CGCG 4 cut(s) 404, 566, 568, 773
BstV1I GCAGC 2 cut(s) 673, 779
BstV2I GAAGAC 1 cut(s) 529
BstX2I RGATCY 1 cut(s) 729
BstYI RGATCY 1 cut(s) 729
BsuRI GGCC 6 cut(s) 183, 201, 219, 239, 356, 416
BtgI CCRYGG 2 cut(s) 40, 357
BtgZI GCGATG 2 cut(s) 121, 214
BtsCI GGATG 3 cut(s) 639, 724, 786
BtsIMutI CAGTG 1 cut(s) 383
Cac8I GCNNGC 3 cut(s) 69, 472, 566
CfoI GCGC 5 cut(s) 102, 406, 500, 566, 568
Cfr10I RCCGGY 3 cut(s) 197, 278, 395
Cfr13I GGNCC 5 cut(s) 105, 200, 237, 354, 743
CseI GACGC 1 cut(s) 202
Csp6I GTAC 2 cut(s) 775, 784
CviAII CATG 3 cut(s) 85, 250, 367
CviQI GTAC 2 cut(s) 775, 784
DdeI CTNAG 3 cut(s) 501, 541, 791
DpnI GATC 4 cut(s) 254, 350, 506, 731
DpnII GATC 4 cut(s) 252, 348, 504, 729
EaeI YGGCCR 1 cut(s) 181
Eam1104I CTCTTC 2 cut(s) 83, 155
EarI CTCTTC 2 cut(s) 83, 155
Ecl136II GAGCTC 2 cut(s) 119, 758
Eco147I AGGCCT 1 cut(s) 416
Eco24I GRGCYC 2 cut(s) 121, 760
Eco31I GGTCTC 1 cut(s) 346
Eco47I GGWCC 2 cut(s) 105, 743
Eco47III AGCGCT 1 cut(s) 101
Eco53kI GAGCTC 2 cut(s) 119, 758
Eco72I CACGTG 1 cut(s) 580
EcoICRI GAGCTC 2 cut(s) 119, 758
EcoRII CCWGG 1 cut(s) 233
EcoT38I GRGCYC 2 cut(s) 121, 760
FaeI CATG 3 cut(s) 88, 253, 370
FaiI YATR 4 cut(s) 86, 125, 251, 368
FatI CATG 3 cut(s) 84, 249, 366
FauI CCCGC 1 cut(s) 463
FblI GTMKAC 2 cut(s) 57, 528
Fnu4HI GCNGC 7 cut(s) 448, 451, 687, 768, 771, 798, 801
FokI GGATG 3 cut(s) 626, 731, 773
FriOI GRGCYC 2 cut(s) 121, 760
Fsp4HI GCNGC 7 cut(s) 448, 451, 687, 768, 771, 798, 801
GlaI GCGC 5 cut(s) 101, 405, 499, 565, 567
GluI GCNGC 7 cut(s) 448, 451, 687, 768, 771, 798, 801
HaeII RGCGCY 2 cut(s) 103, 501
HaeIII GGCC 6 cut(s) 183, 201, 219, 239, 356, 416
HapII CCGG 7 cut(s) 198, 279, 305, 352, 396, 651, 826
HgaI GACGC 1 cut(s) 202
HhaI GCGC 5 cut(s) 102, 406, 500, 566, 568
Hin1I GRCGYC 1 cut(s) 213
Hin1II CATG 3 cut(s) 88, 253, 370
Hin6I GCGC 5 cut(s) 100, 404, 498, 564, 566
HinP1I GCGC 5 cut(s) 100, 404, 498, 564, 566
HincII GTYRAC 1 cut(s) 58
HindII GTYRAC 1 cut(s) 58
HindIII AAGCTT 1 cut(s) 810
HinfI GANTC 1 cut(s) 572
HpaII CCGG 7 cut(s) 198, 279, 305, 352, 396, 651, 826
HphI GGTGA 3 cut(s) 154, 322, 640
Hpy166II GTNNAC 5 cut(s) 34, 58, 268, 529, 628
Hpy188I TCNGA 4 cut(s) 348, 675, 743, 755
Hpy188III TCNNGA 4 cut(s) 421, 436, 790, 826
Hpy8I GTNNAC 5 cut(s) 34, 58, 268, 529, 628
Hpy99I CGWCG 4 cut(s) 59, 170, 212, 218
HpyAV CCTTC 4 cut(s) 118, 230, 427, 757
HpyCH4III ACNGT 3 cut(s) 166, 175, 535
HpyCH4IV ACGT 3 cut(s) 54, 159, 579
HpyCH4V TGCA 3 cut(s) 273, 628, 767
HpyF10VI GCNNNNNNNGC 7 cut(s) 73, 125, 225, 401, 497, 764, 809
HpyF3I CTNAG 3 cut(s) 501, 541, 791
HpySE526I ACGT 3 cut(s) 54, 159, 579
Hsp92I GRCGYC 1 cut(s) 213
Hsp92II CATG 3 cut(s) 88, 253, 370
HspAI GCGC 5 cut(s) 100, 404, 498, 564, 566
Kpn2I TCCGGA 1 cut(s) 825
Kzo9I GATC 4 cut(s) 252, 348, 504, 729
LguI GCTCTTC 1 cut(s) 83
LmnI GCTCC 7 cut(s) 76, 386, 561, 598, 675, 755, 828
Lsp1109I GCAGC 2 cut(s) 673, 779
LweI GCATC 2 cut(s) 237, 669
MaeII ACGT 3 cut(s) 54, 159, 579
MaeIII GTNAC 4 cut(s) 160, 293, 310, 569
MalI GATC 4 cut(s) 254, 350, 506, 731
MbiI CCGCTC 1 cut(s) 76
MboI GATC 4 cut(s) 252, 348, 504, 729
MboII GAAGA 4 cut(s) 70, 142, 529, 724
MflI RGATCY 1 cut(s) 729
MhlI GDGCHC 4 cut(s) 121, 488, 630, 760
MlyI GAGTC 1 cut(s) 566
MmeI TCCRAC 1 cut(s) 721
MnlI CCTC 8 cut(s) 13, 155, 158, 164, 272, 452, 552, 761
MroI TCCGGA 1 cut(s) 825
MseI TTAA 2 cut(s) 96, 300
MslI CAYNNNNRTG 1 cut(s) 518
MspCI CTTAAG 1 cut(s) 95
MspI CCGG 7 cut(s) 198, 279, 305, 352, 396, 651, 826
MspR9I CCNGG 3 cut(s) 235, 305, 353
MvaI CCWGG 1 cut(s) 235
MvnI CGCG 4 cut(s) 404, 566, 568, 773
MwoI GCNNNNNNNGC 7 cut(s) 73, 125, 225, 401, 497, 764, 809
NciI CCSGG 2 cut(s) 305, 353
NdeII GATC 4 cut(s) 252, 348, 504, 729
NlaIII CATG 3 cut(s) 88, 253, 370
NlaIV GGNNCC 7 cut(s) 24, 202, 454, 514, 594, 745, 830
NmeAIII GCCGAG 4 cut(s) 154, 204, 423, 474
NmuCI GTSAC 4 cut(s) 160, 293, 310, 569
OliI CACNNNNGTG 1 cut(s) 518
PauI GCGCGC 1 cut(s) 564
PceI AGGCCT 1 cut(s) 416
PciSI GCTCTTC 1 cut(s) 83
PcsI WCGNNNNNNNCGW 2 cut(s) 87, 687
PflFI GACNNNGTC 2 cut(s) 53, 212
PflMI CCANNNNNTGG 1 cut(s) 382
PkrI GCNGC 7 cut(s) 449, 452, 688, 769, 772, 799, 802
PleI GAGTC 1 cut(s) 566
PmaCI CACGTG 1 cut(s) 580
PmlI CACGTG 1 cut(s) 580
PpsI GAGTC 1 cut(s) 566
Ppu21I YACGTR 1 cut(s) 580
Psp124BI GAGCTC 2 cut(s) 121, 760
Psp6I CCWGG 1 cut(s) 233
PspCI CACGTG 1 cut(s) 580
PspGI CCWGG 1 cut(s) 233
PspN4I GGNNCC 7 cut(s) 24, 202, 454, 514, 594, 745, 830
PspPI GGNCC 5 cut(s) 105, 200, 237, 354, 743
PsuI RGATCY 1 cut(s) 729
PsyI GACNNNGTC 2 cut(s) 53, 212
PteI GCGCGC 1 cut(s) 564
RsaI GTAC 2 cut(s) 776, 785
RsaNI GTAC 2 cut(s) 775, 784
RseI CAYNNNNRTG 1 cut(s) 518
SacI GAGCTC 2 cut(s) 121, 760
SalI GTCGAC 1 cut(s) 56
SapI GCTCTTC 1 cut(s) 83
SaqAI TTAA 2 cut(s) 96, 300
SatI GCNGC 7 cut(s) 448, 451, 687, 768, 771, 798, 801
Sau3AI GATC 4 cut(s) 252, 348, 504, 729
Sau96I GGNCC 5 cut(s) 105, 200, 237, 354, 743
SchI GAGTC 1 cut(s) 566
ScrFI CCNGG 3 cut(s) 235, 305, 353
SduI GDGCHC 4 cut(s) 121, 488, 630, 760
SfaNI GCATC 2 cut(s) 237, 669
SfcI CTRYAG 2 cut(s) 112, 475
SgrAI CRCCGGYG 1 cut(s) 395
SinI GGWCC 2 cut(s) 105, 743
SmiMI CAYNNNNRTG 1 cut(s) 518
SmlI CTYRAG 1 cut(s) 95
SmoI CTYRAG 1 cut(s) 95
SseBI AGGCCT 1 cut(s) 416
SstI GAGCTC 2 cut(s) 121, 760
StuI AGGCCT 1 cut(s) 416
StyD4I CCNGG 3 cut(s) 233, 303, 351
TaaI ACNGT 3 cut(s) 166, 175, 535
TaiI ACGT 3 cut(s) 57, 162, 582
TaqI TCGA 2 cut(s) 57, 81
TatI WGTACW 1 cut(s) 783
TauI GCSGC 5 cut(s) 450, 453, 773, 800, 803
Tru1I TTAA 2 cut(s) 96, 300
Tru9I TTAA 2 cut(s) 96, 300
TscAI CASTG 1 cut(s) 383
TseFI GTSAC 4 cut(s) 160, 293, 310, 569
TseI GCWGC 2 cut(s) 686, 767
Tsp45I GTSAC 4 cut(s) 160, 293, 310, 569
TspDTI ATGAA 2 cut(s) 124, 810
TspRI CASTG 1 cut(s) 383
Tth111I GACNNNGTC 2 cut(s) 53, 212
Van91I CCANNNNNTGG 1 cut(s) 382
Vha464I CTTAAG 1 cut(s) 95
VneI GTGCAC 1 cut(s) 626
VpaK11BI GGWCC 2 cut(s) 105, 743
XcmI CCANNNNNNNNNTGG 1 cut(s) 811
XmiI GTMKAC 2 cut(s) 57, 528
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.