Rorug06G0249600

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0005555
Physical Location & Seq
Forward (+)
39570916 .. 39572019
1104 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0249600.1

Sequence Viewer

Length: 1104 bp
ATGGCCGTCTGCCACCACCAAGTTCCCTTCAAGCTCTTCACTCCGATTCCACCACCAAACCCAAAACCCATCTTCCGATGCAACCTCTCTCTCCCCTCCCGACCCACCCTCCTCACCGCCGATCCCGAACCCCTCTACACCTCCGTCAAGGCCTTCGCCCCCGCCACCGTCGCCAACCTCGGCCCCGGCTTTGACTTCCTCGGATGCGCCGTCGACGGCCTCGGCGACTTCGTCTCCCTCACCGTCGACCCCCAAGTCCTCCCCGGAGAGATCTCCATTTCCGAGATCTCCGGCGACCACAGCTCCAAGAAGCTGAGCAAGAACCCCATCTGGAACTGCGCCGGGATCGCCGCCATCGAGGTCATGAAGATGCTCGGCGTCCGCTCCGTCGGCCTCTCCCTCTCTCTCCAGAAAGGCCTGCCTTTGGGAAGCGGGCTCGGATCCAGCGCGGCCAGCGCCGCCGCCGCCGCCGTCGCCGTCAACGAGATTTTCGGCGGGAAGTTGGGGGTTGAGGCGCTGGTGCACGCCGGGCTCAAATCGGAGGAGAAGGTCTCCGGCTACCACGCCGATAACATCGCTCCGGCGCTGATGGGAGGCTTCGTTCTGATTCAGAATTATGAGCCGTTGGATTTGCTCCGGTTGAGCTTCCCCTTGGACAAAGAGCTGATATTTGTACTGGTGACCCCCGAATTCGAAGCTCCGACGAAGAAAATGAGGGCGGCGCTGCCGGCGGAGATAGGGATGGCCCACCACGTGTGGAATTCCAGCCAGGCCGGAGCGCTGGTGGCGGCGGTGCTTCAAGGTGACGTGGCGAGGCTCGGCAAGGCATTGTCGTCGGATAAGATTGTGGAGCCCAAGAGGGCTCCTCTGATTCCGGGCATGGAGGCGGTGAAGAAGGCCTCCATTGAGGCTGGAGCTTTGGGCTGCACAATCAGCGGGGCCGGGCCCACGGCGGTTGCGGTGACGGACAATGCCGAAAAGGGGAAGGAAATCGGAGAGCGGATGGTGGCGGCTTTTTTGAAAGATGGGAACTTGAAAGCTGTGGCGAGTGTGAATAGGTTGGACAGAGTTGGTGCTAGGCTTGTTAGTAGCATTCCCAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

367

Amino Acids

38.13

Weight (kDa)

8.21

Isoelectric Point (pI)

33.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GHMP_kinases_N PF00288 119 - 199 1.9e-15 GHMP kinases N terminal domain
GHMP_kinases_C PF08544 262 - 338 1.5e-11 GHMP kinases C terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 254
AccBSI CCGCTC 2 cut(s) 384, 1000
AccI GTMKAC 2 cut(s) 213, 246
AccII CGCG 1 cut(s) 449
AclWI GGATC 4 cut(s) 116, 353, 435, 448
AcoI YGGCCR 2 cut(s) 3, 450
AcsI RAATTY 2 cut(s) 689, 760
AcvI CACGTG 1 cut(s) 754
AcyI GRCGYC 1 cut(s) 378
AdeI CACNNNGTG 1 cut(s) 754
AfaI GTAC 1 cut(s) 675
AfeI AGCGCT 1 cut(s) 780
AfiI CCNNNNNNNGG 2 cut(s) 424, 981
AflIII ACRYGT 1 cut(s) 753
AgsI TTSAA 4 cut(s) 31, 800, 1021, 1036
AjiI CACGTC 1 cut(s) 808
AjnI CCWGG 1 cut(s) 768
AloI GAACNNNNNNTCC 2 cut(s) 585, 617
AluBI AGCT 8 cut(s) 34, 303, 313, 645, 664, 698, 917, 1040
AluI AGCT 8 cut(s) 34, 303, 313, 645, 664, 698, 917, 1040
Alw21I GWGCWC 1 cut(s) 525
Alw26I GTCTC 2 cut(s) 238, 556
Alw44I GTGCAC 1 cut(s) 521
AlwI GGATC 4 cut(s) 116, 353, 435, 448
Aor51HI AGCGCT 1 cut(s) 780
ApaI GGGCCC 1 cut(s) 948
ApaLI GTGCAC 1 cut(s) 521
ApeKI GCWGC 2 cut(s) 724, 924
ApoI RAATTY 2 cut(s) 689, 760
AspLEI GCGC 8 cut(s) 209, 341, 449, 458, 517, 586, 724, 781
AspS9I GGNCC 5 cut(s) 182, 745, 939, 944, 945
AsuC2I CCSGG 6 cut(s) 186, 264, 343, 529, 876, 943
AsuHPI GGTGA 6 cut(s) 106, 232, 691, 815, 901, 973
AsuII TTCGAA 1 cut(s) 693
BaeGI GKGCMC 2 cut(s) 525, 948
BamHI GGATCC 1 cut(s) 440
BanII GRGCYC 5 cut(s) 438, 534, 855, 865, 948
BbrPI CACGTG 1 cut(s) 754
Bbv12I GWGCWC 1 cut(s) 525
BbvI GCAGC 2 cut(s) 711, 911
BccI CCATC 7 cut(s) 77, 335, 362, 583, 736, 997, 1019
BceAI ACGGC 6 cut(s) 194, 232, 455, 461, 607, 966
BcgI CGANNNNNNTGC 2 cut(s) 816, 850
BciT130I CCWGG 1 cut(s) 770
BcnI CCSGG 6 cut(s) 186, 264, 343, 529, 876, 943
BcoDI GTCTC 2 cut(s) 238, 556
BfaI CTAG 1 cut(s) 1077
BfoI RGCGCY 5 cut(s) 459, 518, 587, 725, 782
BglII AGATCT 2 cut(s) 270, 285
BlpI GCTNAGC 1 cut(s) 314
Bme1390I CCNGG 7 cut(s) 186, 264, 343, 529, 770, 876, 943
BmgBI CACGTC 1 cut(s) 808
BmgT120I GGNCC 5 cut(s) 182, 745, 939, 944, 945
BmiI GGNNCC 6 cut(s) 184, 442, 852, 864, 940, 946
BmrFI CCNGG 7 cut(s) 186, 264, 343, 529, 770, 876, 943
BmsI GCATC 3 cut(s) 68, 194, 360
BplI GAGNNNNNCTC 4 cut(s) 536, 568, 850, 882
BpmI CTGGAG 2 cut(s) 392, 933
Bpu1102I GCTNAGC 1 cut(s) 314
Bpu14I TTCGAA 1 cut(s) 693
BpuMI CCSGG 6 cut(s) 186, 264, 343, 529, 876, 943
BsaAI YACGTR 1 cut(s) 754
BsaHI GRCGYC 1 cut(s) 378
BsaI GGTCTC 1 cut(s) 556
BsaJI CCNNGG 7 cut(s) 178, 184, 199, 220, 262, 651, 948
BsaWI WCCGGW 1 cut(s) 636
Bsc4I CCNNNNNNNGG 2 cut(s) 424, 981
Bse118I RCCGGY 1 cut(s) 727
Bse1I ACTGG 1 cut(s) 681
BseBI CCWGG 1 cut(s) 770
BseDI CCNNGG 7 cut(s) 178, 184, 199, 220, 262, 651, 948
BseGI GGATG 3 cut(s) 209, 747, 1008
BseLI CCNNNNNNNGG 2 cut(s) 424, 981
BseMII CTCAG 1 cut(s) 305
BseNI ACTGG 1 cut(s) 681
BseRI GAGGAG 3 cut(s) 101, 557, 855
BseSI GKGCMC 2 cut(s) 525, 948
BseXI GCAGC 2 cut(s) 711, 911
BsgI GTGCAG 1 cut(s) 910
Bsh1236I CGCG 1 cut(s) 449
BsiHKAI GWGCWC 1 cut(s) 525
BslI CCNNNNNNNGG 2 cut(s) 424, 981
BsmAI GTCTC 2 cut(s) 238, 556
BsmBI CGTCTC 1 cut(s) 238
BsmI GAATGC 1 cut(s) 1092
Bso31I GGTCTC 1 cut(s) 556
Bsp119I TTCGAA 1 cut(s) 693
Bsp120I GGGCCC 1 cut(s) 944
Bsp1286I GDGCHC 6 cut(s) 438, 525, 534, 855, 865, 948
Bsp143I GATC 5 cut(s) 121, 270, 285, 345, 440
Bsp1720I GCTNAGC 1 cut(s) 314
BspCNI CTCAG 1 cut(s) 306
BspFNI CGCG 1 cut(s) 449
BspHI TCATGA 1 cut(s) 363
BspLI GGNNCC 6 cut(s) 184, 442, 852, 864, 940, 946
BspPI GGATC 4 cut(s) 116, 353, 435, 448
BspQI GCTCTTC 1 cut(s) 41
BspT104I TTCGAA 1 cut(s) 693
BspTNI GGTCTC 1 cut(s) 556
BsrBI CCGCTC 2 cut(s) 384, 1000
BsrFI RCCGGY 1 cut(s) 727
BsrI ACTGG 1 cut(s) 681
BssAI RCCGGY 1 cut(s) 727
BssECI CCNNGG 7 cut(s) 178, 184, 199, 220, 262, 651, 948
BssMI GATC 5 cut(s) 121, 270, 285, 345, 440
BssNI GRCGYC 1 cut(s) 378
BssT1I CCWWGG 1 cut(s) 651
Bst2UI CCWGG 1 cut(s) 770
Bst4CI ACNGT 2 cut(s) 169, 244
Bst6I CTCTTC 1 cut(s) 41
BstACI GRCGYC 1 cut(s) 378
BstBAI YACGTR 1 cut(s) 754
BstBI TTCGAA 1 cut(s) 693
BstC8I GCNNGC 5 cut(s) 419, 434, 454, 525, 729
BstDEI CTNAG 1 cut(s) 314
BstDSI CCRYGG 1 cut(s) 948
BstEII GGTNACC 1 cut(s) 679
BstF5I GGATG 3 cut(s) 209, 747, 1008
BstFNI CGCG 1 cut(s) 449
BstH2I RGCGCY 5 cut(s) 459, 518, 587, 725, 782
BstHHI GCGC 8 cut(s) 209, 341, 449, 458, 517, 586, 724, 781
BstKTI GATC 5 cut(s) 124, 273, 288, 348, 443
BstMAI GTCTC 2 cut(s) 238, 556
BstMBI GATC 5 cut(s) 121, 270, 285, 345, 440
BstNI CCWGG 1 cut(s) 770
BstPI GGTNACC 1 cut(s) 679
BstSCI CCNGG 7 cut(s) 184, 262, 341, 527, 768, 874, 941
BstSLI GKGCMC 2 cut(s) 525, 948
BstUI CGCG 1 cut(s) 449
BstV1I GCAGC 2 cut(s) 711, 911
BstX2I RGATCY 3 cut(s) 270, 285, 440
BstYI RGATCY 3 cut(s) 270, 285, 440
BtgI CCRYGG 1 cut(s) 948
BtgZI GCGATG 1 cut(s) 559
BtrI CACGTC 1 cut(s) 808
BtsCI GGATG 3 cut(s) 209, 747, 1008
Cac8I GCNNGC 5 cut(s) 419, 434, 454, 525, 729
CciI TCATGA 1 cut(s) 363
CfoI GCGC 8 cut(s) 209, 341, 449, 458, 517, 586, 724, 781
Cfr10I RCCGGY 1 cut(s) 727
Cfr13I GGNCC 5 cut(s) 182, 745, 939, 944, 945
CseI GACGC 1 cut(s) 367
Csp6I GTAC 1 cut(s) 674
CspCI CAANNNNNGTGG 2 cut(s) 937, 972
CviAII CATG 2 cut(s) 364, 880
CviQI GTAC 1 cut(s) 674
DdeI CTNAG 1 cut(s) 314
DpnI GATC 5 cut(s) 123, 272, 287, 347, 442
DpnII GATC 5 cut(s) 121, 270, 285, 345, 440
DraIII CACNNNGTG 1 cut(s) 754
DrdI GACNNNNNNGTC 1 cut(s) 254
DseDI GACNNNNNNGTC 1 cut(s) 254
EaeI YGGCCR 2 cut(s) 3, 450
Eam1104I CTCTTC 1 cut(s) 41
EarI CTCTTC 1 cut(s) 41
EciI GGCGGA 1 cut(s) 746
Eco130I CCWWGG 1 cut(s) 651
Eco147I AGGCCT 3 cut(s) 152, 417, 899
Eco24I GRGCYC 5 cut(s) 438, 534, 855, 865, 948
Eco31I GGTCTC 1 cut(s) 556
Eco47III AGCGCT 1 cut(s) 780
Eco72I CACGTG 1 cut(s) 754
Eco91I GGTNACC 1 cut(s) 679
EcoO65I GGTNACC 1 cut(s) 679
EcoRI GAATTC 2 cut(s) 689, 760
EcoRII CCWGG 1 cut(s) 768
EcoT14I CCWWGG 1 cut(s) 651
EcoT38I GRGCYC 5 cut(s) 438, 534, 855, 865, 948
ErhI CCWWGG 1 cut(s) 651
Esp3I CGTCTC 1 cut(s) 238
FaeI CATG 2 cut(s) 367, 883
FaiI YATR 3 cut(s) 365, 618, 881
FatI CATG 2 cut(s) 363, 879
FauI CCCGC 4 cut(s) 169, 425, 488, 929
FblI GTMKAC 2 cut(s) 213, 246
FokI GGATG 3 cut(s) 216, 754, 1015
FriOI GRGCYC 5 cut(s) 438, 534, 855, 865, 948
FspBI CTAG 1 cut(s) 1077
GlaI GCGC 8 cut(s) 208, 340, 448, 457, 516, 585, 723, 780
GsuI CTGGAG 2 cut(s) 392, 933
HaeII RGCGCY 5 cut(s) 459, 518, 587, 725, 782
HgaI GACGC 1 cut(s) 367
HhaI GCGC 8 cut(s) 209, 341, 449, 458, 517, 586, 724, 781
Hin1I GRCGYC 1 cut(s) 378
Hin1II CATG 2 cut(s) 367, 883
Hin6I GCGC 8 cut(s) 207, 339, 447, 456, 515, 584, 722, 779
HinP1I GCGC 8 cut(s) 207, 339, 447, 456, 515, 584, 722, 779
HincII GTYRAC 3 cut(s) 214, 247, 481
HindII GTYRAC 3 cut(s) 214, 247, 481
HinfI GANTC 3 cut(s) 46, 607, 871
HphI GGTGA 6 cut(s) 106, 232, 691, 815, 901, 973
Hpy166II GTNNAC 4 cut(s) 214, 247, 481, 523
Hpy188III TCNNGA 5 cut(s) 99, 125, 331, 364, 409
Hpy8I GTNNAC 4 cut(s) 214, 247, 481, 523
Hpy99I CGWCG 8 cut(s) 173, 215, 218, 248, 392, 476, 706, 838
HpyAV CCTTC 5 cut(s) 37, 163, 541, 889, 979
HpyCH4III ACNGT 2 cut(s) 169, 244
HpyCH4IV ACGT 2 cut(s) 753, 807
HpyCH4V TGCA 3 cut(s) 81, 523, 927
HpyF3I CTNAG 1 cut(s) 314
HpySE526I ACGT 2 cut(s) 753, 807
Hsp92I GRCGYC 1 cut(s) 378
Hsp92II CATG 2 cut(s) 367, 883
HspAI GCGC 8 cut(s) 207, 339, 447, 456, 515, 584, 722, 779
KroI GCCGGC 1 cut(s) 727
KroNI GCCGGC 1 cut(s) 729
Kzo9I GATC 5 cut(s) 121, 270, 285, 345, 440
LguI GCTCTTC 1 cut(s) 41
LmnI GCTCC 9 cut(s) 308, 389, 583, 639, 703, 776, 850, 868, 914
Lsp1109I GCAGC 2 cut(s) 711, 911
LweI GCATC 3 cut(s) 68, 194, 360
MaeI CTAG 1 cut(s) 1077
MaeII ACGT 2 cut(s) 753, 807
MaeIII GTNAC 3 cut(s) 679, 803, 961
MalI GATC 5 cut(s) 123, 272, 287, 347, 442
MbiI CCGCTC 2 cut(s) 384, 1000
MboI GATC 5 cut(s) 121, 270, 285, 345, 440
MboII GAAGA 5 cut(s) 28, 64, 379, 718, 904
MflI RGATCY 3 cut(s) 270, 285, 440
MhlI GDGCHC 6 cut(s) 438, 525, 534, 855, 865, 948
MluCI AATT 3 cut(s) 613, 689, 760
MmeI TCCRAC 4 cut(s) 606, 725, 816, 1041
MroNI GCCGGC 1 cut(s) 727
MslI CAYNNNNRTG 1 cut(s) 368
MspA1I CMGCKG 1 cut(s) 936
MspR9I CCNGG 7 cut(s) 186, 264, 343, 529, 770, 876, 943
Mva1269I GAATGC 1 cut(s) 1092
MvaI CCWGG 1 cut(s) 770
MvnI CGCG 1 cut(s) 449
NaeI GCCGGC 1 cut(s) 729
NciI CCSGG 6 cut(s) 186, 264, 343, 529, 876, 943
NdeII GATC 5 cut(s) 121, 270, 285, 345, 440
NgoMIV GCCGGC 1 cut(s) 727
NlaIII CATG 2 cut(s) 367, 883
NlaIV GGNNCC 6 cut(s) 184, 442, 852, 864, 940, 946
NmeAIII GCCGAG 4 cut(s) 159, 201, 354, 798
NmuCI GTSAC 3 cut(s) 679, 803, 961
NspV TTCGAA 1 cut(s) 693
PagI TCATGA 1 cut(s) 363
PceI AGGCCT 3 cut(s) 152, 417, 899
PciSI GCTCTTC 1 cut(s) 41
PcsI WCGNNNNNNNCGW 5 cut(s) 207, 222, 228, 354, 480
PctI GAATGC 1 cut(s) 1092
PdiI GCCGGC 1 cut(s) 729
PfeI GAWTC 3 cut(s) 46, 607, 871
PflFI GACNNNGTC 1 cut(s) 230
PmaCI CACGTG 1 cut(s) 754
PmlI CACGTG 1 cut(s) 754
Ppu21I YACGTR 1 cut(s) 754
Psp6I CCWGG 1 cut(s) 768
PspCI CACGTG 1 cut(s) 754
PspEI GGTNACC 1 cut(s) 679
PspGI CCWGG 1 cut(s) 768
PspN4I GGNNCC 6 cut(s) 184, 442, 852, 864, 940, 946
PspOMI GGGCCC 1 cut(s) 944
PspPI GGNCC 5 cut(s) 182, 745, 939, 944, 945
PsuI RGATCY 3 cut(s) 270, 285, 440
PsyI GACNNNGTC 1 cut(s) 230
RsaI GTAC 1 cut(s) 675
RsaNI GTAC 1 cut(s) 674
RseI CAYNNNNRTG 1 cut(s) 368
SalI GTCGAC 2 cut(s) 212, 245
SapI GCTCTTC 1 cut(s) 41
Sau3AI GATC 5 cut(s) 121, 270, 285, 345, 440
Sau96I GGNCC 5 cut(s) 182, 745, 939, 944, 945
ScrFI CCNGG 7 cut(s) 186, 264, 343, 529, 770, 876, 943
SduI GDGCHC 6 cut(s) 438, 525, 534, 855, 865, 948
SfaNI GCATC 3 cut(s) 68, 194, 360
SfuI TTCGAA 1 cut(s) 693
SgrDI CGTCGACG 1 cut(s) 212
SmiMI CAYNNNNRTG 1 cut(s) 368
Sse9I AATT 3 cut(s) 613, 689, 760
SseBI AGGCCT 3 cut(s) 152, 417, 899
SspMI CTAG 1 cut(s) 1077
StuI AGGCCT 3 cut(s) 152, 417, 899
StyD4I CCNGG 7 cut(s) 184, 262, 341, 527, 768, 874, 941
StyI CCWWGG 1 cut(s) 651
TaaI ACNGT 2 cut(s) 169, 244
TaiI ACGT 2 cut(s) 756, 810
TaqI TCGA 4 cut(s) 213, 246, 357, 693
TasI AATT 3 cut(s) 613, 689, 760
TatI WGTACW 1 cut(s) 673
TauI GCSGC 9 cut(s) 353, 452, 461, 464, 467, 470, 722, 791, 1013
TfiI GAWTC 3 cut(s) 46, 607, 871
TseFI GTSAC 3 cut(s) 679, 803, 961
TseI GCWGC 2 cut(s) 724, 924
Tsp45I GTSAC 3 cut(s) 679, 803, 961
TspDTI ATGAA 1 cut(s) 380
TspGWI ACGGA 3 cut(s) 133, 376, 980
Tth111I GACNNNGTC 1 cut(s) 230
VneI GTGCAC 1 cut(s) 521
XapI RAATTY 2 cut(s) 689, 760
XmiI GTMKAC 2 cut(s) 213, 246
XspI CTAG 1 cut(s) 1077
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.