Rorug06G0500700
MADS Family

MADS-box transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0011560
Physical Location & Seq
Reverse (-)
61609571 .. 61611329
1759 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0500700.1

Sequence Viewer

Length: 1071 bp
ATGCAGGCACCGTACGATGCTACCGTTCGATTCGCGCTGGCGTCACTGGAGAGGAATCTGTTACCTGACGCCGTTATAAGGCGGTTCACGCGCTTGCTCTTAGCGAGTCGGCTACGCTCCGGTTACCAGCCTTCCTCCGAGCTCCAGCTCTCCCAACTCCTCCAGTTCGTAAAGTCTCTAAAAGAGATGCCAATAGCTATCAAGACTGATGACGCTAAAGCTCAACACTATGAAGTACCCACTTCTTTCTTCAAGATGGTGCTTGGGAACAATCTCAAATATAGTTGCTGTTACTTCACTGATAAGTCAAGCAGCTTGGAGGATGCGGAGAAGGCCATGTTGGAGATGTACTGTGAGAGATCGCAGATTAAAGATGGCCATACTGTTCTTGATGTTGGATGTGGATGGGGATCACTATCTCTGTACATTGCACAGAAATATAAGAATTGCAGGGTTACCGGGATCTGCAATTCAGCAACACAGAAGGCTTTTATAGAAGAGCAGTGTAGGAATCGTCGGCTGGAGAATGTGGAGATCATTGTTGCAGATATCAGCACATTTGAGATGGAGGCATCATATGACAGAATATTTTCCATCGAAATGTTCGAGCACATGAAGAACTACAAGGATCTTCTGAAGAAGATATCCGGGTGGATGAAACAGGACGGCCTTCTGTTTGTACATCATTTCTGTCACAAAGCATTTGCTTACCACTTTGAGGACAAAAGTGATGATGACTGGATTACTAGGTACTTCTTCAGTGGAGGTACAATGCCTTCAGCAAATCTACTACTGTATTTCCAGCGATCTTTTATGCAGGATGATGTTTCTGTTGTCGATCATTGGCTTGTCAATGGGAAACATTATGCACAAACAAGTGAAGAATGGCTGAAAAGAATGGATCAGAATTTGGCTTCCATAAAACCAATTATGGAGTCAACTTATGGCAAGGATTCGGCTGTGAAGTGGACTGTGTATTGGAGAACTTTCTTTATTGCAGTTGCAGAACTCTTTGGCTACAACAACGGAGAAGAATGGATGGTTGCTCTCTTCTTATTCAAAAAGAAATGA

Protein Analysis

356

Amino Acids

41.48

Weight (kDa)

7.1

Isoelectric Point (pI)

40.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CMAS PF02353 71 - 313 4.2e-46 Mycolic acid cyclopropane synthetase
PCMT PF01135 112 - 197 1e-05 Protein-L-isoaspartate(D-aspartate) O-methyltransferase (PCMT)
TRM5-TYW2_MTfase PF02475 121 - 234 2.4e-06 TRM5/TYW2 methyltransferase domain
Methyltransf_31 PF13847 123 - 244 4.9e-15 Methyltransferase domain
Methyltransf_23 PF13489 123 - 232 9.3e-12 Methyltransferase domain
TehB PF03848 123 - 209 2e-06 Tellurite resistance protein TehB
MTS PF05175 125 - 198 3.3e-07 Methyltransferase small domain
Methyltransf_25 PF13649 129 - 223 7.4e-16 Methyltransferase domain
Methyltransf_11 PF08241 130 - 227 5.6e-15 Methyltransferase domain
Methyltransf_12 PF08242 130 - 225 3.4e-11 Methyltransferase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 77
AccB1I GGYRCC 1 cut(s) 7
AccII CGCG 2 cut(s) 35, 91
AciI CCGC 2 cut(s) 82, 326
AclWI GGATC 4 cut(s) 418, 470, 636, 909
AcoI YGGCCR 1 cut(s) 376
AcsI RAATTY 1 cut(s) 907
AcuI CTGAAG 3 cut(s) 656, 742, 762
AcyI GRCGYC 2 cut(s) 41, 69
AfaI GTAC 7 cut(s) 14, 237, 350, 425, 681, 752, 769
AfiI CCNNNNNNNGG 2 cut(s) 78, 718
AgsI TTSAA 2 cut(s) 253, 1060
AjuI GAANNNNNNNTTGG 2 cut(s) 323, 355
AluBI AGCT 5 cut(s) 142, 148, 197, 221, 315
AluI AGCT 5 cut(s) 142, 148, 197, 221, 315
Alw21I GWGCWC 2 cut(s) 144, 612
Alw26I GTCTC 1 cut(s) 180
AlwI GGATC 4 cut(s) 418, 470, 636, 909
AoxI GGCC 3 cut(s) 333, 376, 667
ApeKI GCWGC 1 cut(s) 312
ApoI RAATTY 1 cut(s) 907
Asp700I GAANNNNTTC 1 cut(s) 589
AspLEI GCGC 2 cut(s) 37, 93
AsuC2I CCSGG 2 cut(s) 460, 649
BalI TGGCCA 1 cut(s) 378
BanI GGYRCC 1 cut(s) 7
BanII GRGCYC 1 cut(s) 144
BarI GAAGNNNNNNTAC 2 cut(s) 760, 792
Bbv12I GWGCWC 2 cut(s) 144, 612
BbvI GCAGC 1 cut(s) 324
BccI CCATC 6 cut(s) 250, 368, 399, 559, 602, 1033
BceAI ACGGC 2 cut(s) 56, 682
BcnI CCSGG 2 cut(s) 460, 649
BcoDI GTCTC 1 cut(s) 180
BfaI CTAG 1 cut(s) 747
BisI GCNGC 1 cut(s) 313
BlsI GCNGC 1 cut(s) 314
Bme1390I CCNGG 2 cut(s) 460, 649
BmiI GGNNCC 1 cut(s) 9
BmrFI CCNGG 2 cut(s) 460, 649
BmsI GCATC 4 cut(s) 7, 177, 313, 581
BpmI CTGGAG 4 cut(s) 68, 128, 146, 542
BpuMI CCSGG 2 cut(s) 460, 649
BsaBI GATNNNNATC 2 cut(s) 409, 415
BsaHI GRCGYC 2 cut(s) 41, 69
BsaWI WCCGGW 1 cut(s) 119
Bsc4I CCNNNNNNNGG 2 cut(s) 78, 718
Bse1I ACTGG 3 cut(s) 51, 163, 743
Bse3DI GCAATG 1 cut(s) 426
Bse8I GATNNNNATC 2 cut(s) 409, 415
BseGI GGATG 6 cut(s) 328, 404, 410, 660, 826, 1044
BseJI GATNNNNATC 2 cut(s) 409, 415
BseLI CCNNNNNNNGG 2 cut(s) 78, 718
BseMI GCAATG 1 cut(s) 426
BseNI ACTGG 3 cut(s) 51, 163, 743
BseRI GAGGAG 1 cut(s) 149
BseXI GCAGC 1 cut(s) 324
Bsh1236I CGCG 2 cut(s) 35, 91
BshFI GGCC 3 cut(s) 335, 378, 669
BshNI GGYRCC 1 cut(s) 7
BsiHKAI GWGCWC 2 cut(s) 144, 612
BsiSI CCGG 3 cut(s) 120, 459, 648
BsiWI CGTACG 1 cut(s) 12
BslI CCNNNNNNNGG 2 cut(s) 78, 718
BsmAI GTCTC 1 cut(s) 180
BsnI GGCC 3 cut(s) 335, 378, 669
Bsp1286I GDGCHC 2 cut(s) 144, 612
Bsp1407I TGTACA 2 cut(s) 423, 679
Bsp143I GATC 8 cut(s) 359, 410, 462, 534, 628, 806, 838, 901
BspACI CCGC 2 cut(s) 82, 326
BspANI GGCC 3 cut(s) 335, 378, 669
BspFNI CGCG 2 cut(s) 35, 91
BspLI GGNNCC 1 cut(s) 9
BspPI GGATC 4 cut(s) 418, 470, 636, 909
BspQI GCTCTTC 1 cut(s) 492
BspT107I GGYRCC 1 cut(s) 7
BsrDI GCAATG 1 cut(s) 426
BsrGI TGTACA 2 cut(s) 423, 679
BsrI ACTGG 3 cut(s) 51, 163, 743
BssMI GATC 8 cut(s) 359, 410, 462, 534, 628, 806, 838, 901
BssNI GRCGYC 2 cut(s) 41, 69
Bst4CI ACNGT 6 cut(s) 12, 25, 353, 385, 795, 973
Bst6I CTCTTC 2 cut(s) 492, 1055
BstACI GRCGYC 2 cut(s) 41, 69
BstAUI TGTACA 2 cut(s) 423, 679
BstC8I GCNNGC 3 cut(s) 6, 39, 95
BstDEI CTNAG 1 cut(s) 100
BstEII GGTNACC 2 cut(s) 122, 454
BstF5I GGATG 6 cut(s) 328, 404, 410, 660, 826, 1044
BstFNI CGCG 2 cut(s) 35, 91
BstHHI GCGC 2 cut(s) 37, 93
BstKTI GATC 8 cut(s) 362, 413, 465, 537, 631, 809, 841, 904
BstMAI GTCTC 1 cut(s) 180
BstMBI GATC 8 cut(s) 359, 410, 462, 534, 628, 806, 838, 901
BstMWI GCNNNNNNNGC 2 cut(s) 88, 332
BstPI GGTNACC 2 cut(s) 122, 454
BstSCI CCNGG 2 cut(s) 458, 647
BstUI CGCG 2 cut(s) 35, 91
BstV1I GCAGC 1 cut(s) 324
BstX2I RGATCY 2 cut(s) 462, 628
BstYI RGATCY 2 cut(s) 462, 628
BsuRI GGCC 3 cut(s) 335, 378, 669
BtsCI GGATG 6 cut(s) 328, 404, 410, 660, 826, 1044
BtsI GCAGTG 1 cut(s) 509
BtsIMutI CAGTG 4 cut(s) 44, 297, 509, 766
Cac8I GCNNGC 3 cut(s) 6, 39, 95
CfoI GCGC 2 cut(s) 37, 93
CseI GACGC 3 cut(s) 30, 77, 221
Csp6I GTAC 7 cut(s) 13, 236, 349, 424, 680, 751, 768
CviAII CATG 2 cut(s) 337, 613
CviQI GTAC 7 cut(s) 13, 236, 349, 424, 680, 751, 768
DdeI CTNAG 1 cut(s) 100
DpnI GATC 8 cut(s) 361, 412, 464, 536, 630, 808, 840, 903
DpnII GATC 8 cut(s) 359, 410, 462, 534, 628, 806, 838, 901
EaeI YGGCCR 1 cut(s) 376
Eam1104I CTCTTC 2 cut(s) 492, 1055
EarI CTCTTC 2 cut(s) 492, 1055
Ecl136II GAGCTC 1 cut(s) 142
Eco24I GRGCYC 1 cut(s) 144
Eco32I GATATC 2 cut(s) 550, 645
Eco53kI GAGCTC 1 cut(s) 142
Eco57I CTGAAG 3 cut(s) 656, 742, 762
Eco91I GGTNACC 2 cut(s) 122, 454
EcoICRI GAGCTC 1 cut(s) 142
EcoO65I GGTNACC 2 cut(s) 122, 454
EcoRV GATATC 2 cut(s) 550, 645
EcoT38I GRGCYC 1 cut(s) 144
FaeI CATG 2 cut(s) 340, 616
FatI CATG 2 cut(s) 336, 612
FauNDI CATATG 1 cut(s) 577
Fnu4HI GCNGC 1 cut(s) 313
FokI GGATG 6 cut(s) 335, 411, 417, 667, 833, 1051
FriOI GRGCYC 1 cut(s) 144
Fsp4HI GCNGC 1 cut(s) 313
FspBI CTAG 1 cut(s) 747
GlaI GCGC 2 cut(s) 36, 92
GluI GCNGC 1 cut(s) 313
GsuI CTGGAG 4 cut(s) 68, 128, 146, 542
HaeIII GGCC 3 cut(s) 335, 378, 669
HapII CCGG 3 cut(s) 120, 459, 648
HgaI GACGC 3 cut(s) 30, 77, 221
HhaI GCGC 2 cut(s) 37, 93
Hin1I GRCGYC 2 cut(s) 41, 69
Hin1II CATG 2 cut(s) 340, 616
Hin6I GCGC 2 cut(s) 35, 91
HinP1I GCGC 2 cut(s) 35, 91
HincII GTYRAC 1 cut(s) 939
HindII GTYRAC 1 cut(s) 939
HinfI GANTC 6 cut(s) 30, 55, 106, 511, 935, 953
HpaII CCGG 3 cut(s) 120, 459, 648
Hpy166II GTNNAC 3 cut(s) 87, 939, 969
Hpy188I TCNGA 3 cut(s) 139, 636, 906
Hpy188III TCNNGA 3 cut(s) 202, 253, 389
Hpy8I GTNNAC 3 cut(s) 87, 939, 969
Hpy99I CGWCG 1 cut(s) 519
HpyAV CCTTC 5 cut(s) 141, 325, 478, 680, 786
HpyCH4III ACNGT 6 cut(s) 12, 25, 353, 385, 795, 973
HpyCH4V TGCA 9 cut(s) 4, 431, 450, 468, 545, 817, 869, 998, 1004
HpyF10VI GCNNNNNNNGC 2 cut(s) 88, 332
HpyF3I CTNAG 1 cut(s) 100
Hsp92I GRCGYC 2 cut(s) 41, 69
Hsp92II CATG 2 cut(s) 340, 616
HspAI GCGC 2 cut(s) 35, 91
Kzo9I GATC 8 cut(s) 359, 410, 462, 534, 628, 806, 838, 901
LguI GCTCTTC 1 cut(s) 492
LmnI GCTCC 2 cut(s) 122, 147
Lsp1109I GCAGC 1 cut(s) 324
LweI GCATC 4 cut(s) 7, 177, 313, 581
MaeI CTAG 1 cut(s) 747
MaeIII GTNAC 6 cut(s) 42, 60, 122, 290, 454, 692
MalI GATC 8 cut(s) 361, 412, 464, 536, 630, 808, 840, 903
MboI GATC 8 cut(s) 359, 410, 462, 534, 628, 806, 838, 901
MflI RGATCY 2 cut(s) 462, 628
MhlI GDGCHC 2 cut(s) 144, 612
MlsI TGGCCA 1 cut(s) 378
MluCI AATT 4 cut(s) 445, 469, 907, 927
MluNI TGGCCA 1 cut(s) 378
MlyI GAGTC 2 cut(s) 115, 944
MmeI TCCRAC 2 cut(s) 321, 376
MnlI CCTC 7 cut(s) 45, 145, 170, 313, 562, 712, 758
Mox20I TGGCCA 1 cut(s) 378
MroXI GAANNNNTTC 1 cut(s) 589
MscI TGGCCA 1 cut(s) 378
MseI TTAA 1 cut(s) 369
MslI CAYNNNNRTG 1 cut(s) 599
Msp20I TGGCCA 1 cut(s) 378
MspI CCGG 3 cut(s) 120, 459, 648
MspR9I CCNGG 2 cut(s) 460, 649
MvnI CGCG 2 cut(s) 35, 91
MwoI GCNNNNNNNGC 2 cut(s) 88, 332
NciI CCSGG 2 cut(s) 460, 649
NdeI CATATG 1 cut(s) 577
NdeII GATC 8 cut(s) 359, 410, 462, 534, 628, 806, 838, 901
NlaIII CATG 2 cut(s) 340, 616
NlaIV GGNNCC 1 cut(s) 9
NmuCI GTSAC 2 cut(s) 42, 692
PciSI GCTCTTC 1 cut(s) 492
PcsI WCGNNNNNNNCGW 2 cut(s) 21, 603
PdmI GAANNNNTTC 1 cut(s) 589
PfeI GAWTC 4 cut(s) 30, 55, 511, 953
Pfl23II CGTACG 1 cut(s) 12
PkrI GCNGC 1 cut(s) 314
PleI GAGTC 2 cut(s) 114, 943
PpsI GAGTC 2 cut(s) 114, 943
PsiI TTATAA 1 cut(s) 77
Psp124BI GAGCTC 1 cut(s) 144
PspEI GGTNACC 2 cut(s) 122, 454
PspLI CGTACG 1 cut(s) 12
PspN4I GGNNCC 1 cut(s) 9
PsuI RGATCY 2 cut(s) 462, 628
RsaI GTAC 7 cut(s) 14, 237, 350, 425, 681, 752, 769
RsaNI GTAC 7 cut(s) 13, 236, 349, 424, 680, 751, 768
RseI CAYNNNNRTG 1 cut(s) 599
SacI GAGCTC 1 cut(s) 144
SapI GCTCTTC 1 cut(s) 492
SaqAI TTAA 1 cut(s) 369
SatI GCNGC 1 cut(s) 313
Sau3AI GATC 8 cut(s) 359, 410, 462, 534, 628, 806, 838, 901
SchI GAGTC 2 cut(s) 115, 944
ScrFI CCNGG 2 cut(s) 460, 649
SduI GDGCHC 2 cut(s) 144, 612
SetI ASST 8 cut(s) 67, 144, 150, 199, 223, 317, 752, 769
SfaNI GCATC 4 cut(s) 7, 177, 313, 581
SmiMI CAYNNNNRTG 1 cut(s) 599
Sse9I AATT 4 cut(s) 445, 469, 907, 927
SsiI CCGC 2 cut(s) 82, 326
SspI AATATT 1 cut(s) 588
SspMI CTAG 1 cut(s) 747
SstI GAGCTC 1 cut(s) 144
StyD4I CCNGG 2 cut(s) 458, 647
TaaI ACNGT 6 cut(s) 12, 25, 353, 385, 795, 973
TaqI TCGA 4 cut(s) 28, 597, 606, 837
TasI AATT 4 cut(s) 445, 469, 907, 927
TatI WGTACW 3 cut(s) 348, 423, 679
TfiI GAWTC 4 cut(s) 30, 55, 511, 953
Tru1I TTAA 1 cut(s) 369
Tru9I TTAA 1 cut(s) 369
TscAI CASTG 4 cut(s) 51, 304, 509, 766
TseFI GTSAC 2 cut(s) 42, 692
TseI GCWGC 1 cut(s) 312
Tsp45I GTSAC 2 cut(s) 42, 692
TspDTI ATGAA 3 cut(s) 246, 629, 671
TspGWI ACGGA 1 cut(s) 1041
TspRI CASTG 4 cut(s) 51, 304, 509, 766
XapI RAATTY 1 cut(s) 907
XmnI GAANNNNTTC 1 cut(s) 589
XspI CTAG 1 cut(s) 747
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.