Rorug07G0272400

tRNA m(4)X modification enzyme TRM13 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000007
OG0006802
Physical Location & Seq
Reverse (-)
25816769 .. 25817761
993 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug07G0272400.1

Sequence Viewer

Length: 729 bp
ATGGTCGATTCAGCTCAAGATATTGAGATTGATTCGAAATCGGCTCAAACGGAGCCAGGTCCTCCGCCGTCACTACAGTGCATGTTGCGGCGCAAGGATGCCACCGGCGGTTGCGCAAGGTCTAGGCGAAGCTACTGGAGGTGGTCCGACGTCTTGAGGTGGCCTGAGGAGAGAGAGAAACAACCGGGTATTACAGTTCGGGTTTCGTGCATAGTCAATGCACGTGGGAAGCGCAACAGTCTACGTCTTCCTTTGGGTTACTACGGCAATGCATTCGTATACCCAGCTGCTGTTTCGGGAGTGAAACAATTGTGTGAAAGTCCGTTGGGATATGCACTGGAGTTGGTGATGAAGGCAAAAAATGAAATGAATGAAGAGTACATGAGATCAGTGGCAGATCTTATGGTCATAAAAGGACAGCCTCCATATACATTGACCGGTAACTTCACAGTTTCAGACAATAGACGTTTTGGCCTTGCAGATGTCAACTTGGGGTGGGGAAAGCCAACGTTTTCTGGACCTGCCAAGGCCTTGAAGTTTCTTAGTTTCTATATTCAGCACAAAAAGGAAAAAGAGGACGGGATTTTGGTGCCAGTGTGCTTGCCATCGTGGAGTATGGCGAGATTTCAGCAGGAACTCGAGAAGATGACGTCGTCGGAGATAGTGGGAAATATAAATAACACTAAATCTGCTAATATTAATGTTGCAAGGATGATTCCACGGTTGTAA

Protein Analysis

242

Amino Acids

27.17

Weight (kDa)

9.45

Isoelectric Point (pI)

68.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Transferase PF02458 59 - 216 4.4e-19 Transferase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 2 cut(s) 153, 653
Acc16I TGCGCA 1 cut(s) 115
Acc36I ACCTGC 1 cut(s) 529
AccB1I GGYRCC 1 cut(s) 589
AccI GTMKAC 2 cut(s) 241, 279
AciI CCGC 3 cut(s) 65, 88, 108
AclI AACGTT 1 cut(s) 509
AcvI CACGTG 1 cut(s) 224
AcyI GRCGYC 2 cut(s) 150, 650
AfaI GTAC 1 cut(s) 380
AgeI ACCGGT 1 cut(s) 437
AgsI TTSAA 1 cut(s) 535
AjnI CCWGG 1 cut(s) 55
AleI CACNNNNGTG 1 cut(s) 76
AluBI AGCT 3 cut(s) 14, 132, 287
AluI AGCT 3 cut(s) 14, 132, 287
AlwNI CAGNNNCTG 1 cut(s) 290
Ama87I CYCGRG 1 cut(s) 638
AoxI GGCC 3 cut(s) 161, 472, 528
ApeKI GCWGC 1 cut(s) 287
AseI ATTAAT 1 cut(s) 699
AsiGI ACCGGT 1 cut(s) 437
AspLEI GCGC 3 cut(s) 93, 116, 234
AspS9I GGNCC 3 cut(s) 59, 144, 518
AsuC2I CCSGG 1 cut(s) 186
AsuHPI GGTGA 1 cut(s) 358
AsuII TTCGAA 1 cut(s) 35
AvaI CYCGRG 1 cut(s) 638
AvaII GGWCC 3 cut(s) 59, 144, 518
AxyI CCTNAGG 1 cut(s) 165
BanI GGYRCC 1 cut(s) 589
BbrPI CACGTG 1 cut(s) 224
BbsI GAAGAC 1 cut(s) 239
BbvI GCAGC 1 cut(s) 274
BccI CCATC 1 cut(s) 613
BceAI ACGGC 2 cut(s) 52, 280
BcgI CGANNNNNNTGC 2 cut(s) 256, 290
BciT130I CCWGG 1 cut(s) 57
BcnI CCSGG 1 cut(s) 186
BfaI CTAG 1 cut(s) 123
BfmI CTRYAG 1 cut(s) 74
BfuAI ACCTGC 1 cut(s) 529
BglII AGATCT 1 cut(s) 397
BisI GCNGC 2 cut(s) 89, 288
BlsI GCNGC 2 cut(s) 90, 289
Bme1390I CCNGG 2 cut(s) 57, 186
Bme18I GGWCC 3 cut(s) 59, 144, 518
BmeT110I CYCGRG 1 cut(s) 638
BmgT120I GGNCC 3 cut(s) 59, 144, 518
BmiI GGNNCC 2 cut(s) 54, 591
BmrFI CCNGG 2 cut(s) 57, 186
BmsI GCATC 1 cut(s) 88
BpiI GAAGAC 1 cut(s) 239
BpmI CTGGAG 2 cut(s) 157, 359
Bpu14I TTCGAA 1 cut(s) 35
BpuEI CTTGAG 1 cut(s) 175
BpuMI CCSGG 1 cut(s) 186
BsaAI YACGTR 1 cut(s) 224
BsaHI GRCGYC 2 cut(s) 150, 650
BsaJI CCNNGG 2 cut(s) 525, 719
BsaWI WCCGGW 1 cut(s) 437
Bse118I RCCGGY 2 cut(s) 104, 437
Bse1I ACTGG 3 cut(s) 140, 342, 593
Bse21I CCTNAGG 1 cut(s) 165
Bse3DI GCAATG 1 cut(s) 274
BseBI CCWGG 1 cut(s) 57
BseDI CCNNGG 2 cut(s) 525, 719
BseGI GGATG 2 cut(s) 103, 717
BseMI GCAATG 1 cut(s) 274
BseMII CTCAG 1 cut(s) 156
BseNI ACTGG 3 cut(s) 140, 342, 593
BseRI GAGGAG 1 cut(s) 182
BseXI GCAGC 1 cut(s) 274
BseYI CCCAGC 1 cut(s) 283
BshFI GGCC 3 cut(s) 163, 474, 530
BshNI GGYRCC 1 cut(s) 589
BshTI ACCGGT 1 cut(s) 437
BsiHKCI CYCGRG 1 cut(s) 638
BsiSI CCGG 3 cut(s) 105, 185, 438
BsmI GAATGC 1 cut(s) 272
BsnI GGCC 3 cut(s) 163, 474, 530
BsoBI CYCGRG 1 cut(s) 638
Bsp119I TTCGAA 1 cut(s) 35
Bsp143I GATC 2 cut(s) 386, 397
BspACI CCGC 3 cut(s) 65, 88, 108
BspANI GGCC 3 cut(s) 163, 474, 530
BspCNI CTCAG 1 cut(s) 157
BspLI GGNNCC 2 cut(s) 54, 591
BspMI ACCTGC 1 cut(s) 529
BspT104I TTCGAA 1 cut(s) 35
BspT107I GGYRCC 1 cut(s) 589
BsrDI GCAATG 1 cut(s) 274
BsrFI RCCGGY 2 cut(s) 104, 437
BsrI ACTGG 3 cut(s) 140, 342, 593
BssAI RCCGGY 2 cut(s) 104, 437
BssECI CCNNGG 2 cut(s) 525, 719
BssMI GATC 2 cut(s) 386, 397
BssNAI GTATAC 1 cut(s) 280
BssNI GRCGYC 2 cut(s) 150, 650
BssT1I CCWWGG 1 cut(s) 525
Bst1107I GTATAC 1 cut(s) 280
Bst2UI CCWGG 1 cut(s) 57
Bst4CI ACNGT 5 cut(s) 78, 196, 239, 451, 723
Bst6I CTCTTC 1 cut(s) 369
BstACI GRCGYC 2 cut(s) 150, 650
BstBAI YACGTR 1 cut(s) 224
BstBI TTCGAA 1 cut(s) 35
BstC8I GCNNGC 1 cut(s) 602
BstDEI CTNAG 2 cut(s) 165, 542
BstDSI CCRYGG 1 cut(s) 719
BstF5I GGATG 2 cut(s) 103, 717
BstHHI GCGC 3 cut(s) 93, 116, 234
BstKTI GATC 2 cut(s) 389, 400
BstMBI GATC 2 cut(s) 386, 397
BstNI CCWGG 1 cut(s) 57
BstNSI RCATGY 1 cut(s) 85
BstSCI CCNGG 2 cut(s) 55, 184
BstSFI CTRYAG 1 cut(s) 74
BstV1I GCAGC 1 cut(s) 274
BstV2I GAAGAC 1 cut(s) 239
BstX2I RGATCY 1 cut(s) 397
BstYI RGATCY 1 cut(s) 397
BstZ17I GTATAC 1 cut(s) 280
Bsu36I CCTNAGG 1 cut(s) 165
BsuRI GGCC 3 cut(s) 163, 474, 530
BtgI CCRYGG 1 cut(s) 719
BtsCI GGATG 2 cut(s) 103, 717
BtsIMutI CAGTG 4 cut(s) 83, 335, 396, 600
BveI ACCTGC 1 cut(s) 529
Cac8I GCNNGC 1 cut(s) 602
CaiI CAGNNNCTG 1 cut(s) 290
CfoI GCGC 3 cut(s) 93, 116, 234
Cfr10I RCCGGY 2 cut(s) 104, 437
Cfr13I GGNCC 3 cut(s) 59, 144, 518
Csp6I GTAC 1 cut(s) 379
CspAI ACCGGT 1 cut(s) 437
CspCI CAANNNNNGTGG 2 cut(s) 205, 240
CviAII CATG 2 cut(s) 82, 382
CviQI GTAC 1 cut(s) 379
DdeI CTNAG 2 cut(s) 165, 542
DpnI GATC 2 cut(s) 388, 399
DpnII GATC 2 cut(s) 386, 397
Eam1104I CTCTTC 1 cut(s) 369
EarI CTCTTC 1 cut(s) 369
EciI GGCGGA 1 cut(s) 54
Eco130I CCWWGG 1 cut(s) 525
Eco147I AGGCCT 1 cut(s) 530
Eco47I GGWCC 3 cut(s) 59, 144, 518
Eco72I CACGTG 1 cut(s) 224
Eco81I CCTNAGG 1 cut(s) 165
Eco88I CYCGRG 1 cut(s) 638
EcoO109I RGGNCCY 1 cut(s) 59
EcoRII CCWGG 1 cut(s) 55
EcoT14I CCWWGG 1 cut(s) 525
EcoT22I ATGCAT 1 cut(s) 274
ErhI CCWWGG 1 cut(s) 525
FaeI CATG 2 cut(s) 85, 385
FatI CATG 2 cut(s) 81, 381
FblI GTMKAC 2 cut(s) 241, 279
Fnu4HI GCNGC 2 cut(s) 89, 288
FokI GGATG 2 cut(s) 110, 724
Fsp4HI GCNGC 2 cut(s) 89, 288
FspBI CTAG 1 cut(s) 123
FspI TGCGCA 1 cut(s) 115
GlaI GCGC 3 cut(s) 92, 115, 233
GluI GCNGC 2 cut(s) 89, 288
GsaI CCCAGC 1 cut(s) 287
GsuI CTGGAG 2 cut(s) 157, 359
HaeIII GGCC 3 cut(s) 163, 474, 530
HapII CCGG 3 cut(s) 105, 185, 438
HhaI GCGC 3 cut(s) 93, 116, 234
Hin1I GRCGYC 2 cut(s) 150, 650
Hin1II CATG 2 cut(s) 85, 385
Hin6I GCGC 3 cut(s) 91, 114, 232
HinP1I GCGC 3 cut(s) 91, 114, 232
HincII GTYRAC 1 cut(s) 487
HindII GTYRAC 1 cut(s) 487
HinfI GANTC 3 cut(s) 8, 32, 715
HpaII CCGG 3 cut(s) 105, 185, 438
HphI GGTGA 1 cut(s) 358
Hpy166II GTNNAC 3 cut(s) 242, 280, 487
Hpy188I TCNGA 3 cut(s) 148, 457, 658
Hpy188III TCNNGA 5 cut(s) 17, 154, 297, 516, 640
Hpy8I GTNNAC 3 cut(s) 242, 280, 487
Hpy99I CGWCG 3 cut(s) 152, 655, 658
HpyAV CCTTC 1 cut(s) 346
HpyCH4III ACNGT 5 cut(s) 78, 196, 239, 451, 723
HpyCH4IV ACGT 6 cut(s) 150, 223, 244, 466, 509, 650
HpyCH4V TGCA 7 cut(s) 81, 210, 221, 272, 335, 479, 707
HpyF3I CTNAG 2 cut(s) 165, 542
HpySE526I ACGT 6 cut(s) 150, 223, 244, 466, 509, 650
Hsp92I GRCGYC 2 cut(s) 150, 650
Hsp92II CATG 2 cut(s) 85, 385
HspAI GCGC 3 cut(s) 91, 114, 232
Kzo9I GATC 2 cut(s) 386, 397
LmnI GCTCC 1 cut(s) 52
Lsp1109I GCAGC 1 cut(s) 274
LweI GCATC 1 cut(s) 88
MaeI CTAG 1 cut(s) 123
MaeII ACGT 6 cut(s) 150, 223, 244, 466, 509, 650
MaeIII GTNAC 3 cut(s) 69, 257, 440
MalI GATC 2 cut(s) 388, 399
MboI GATC 2 cut(s) 386, 397
MboII GAAGA 3 cut(s) 239, 386, 655
MfeI CAATTG 1 cut(s) 308
MflI RGATCY 1 cut(s) 397
MluCI AATT 1 cut(s) 308
MmeI TCCRAC 2 cut(s) 171, 636
MnlI CCTC 6 cut(s) 72, 132, 150, 160, 432, 568
Mph1103I ATGCAT 1 cut(s) 274
MseI TTAA 1 cut(s) 699
MslI CAYNNNNRTG 1 cut(s) 76
MspA1I CMGCKG 1 cut(s) 287
MspI CCGG 3 cut(s) 105, 185, 438
MspR9I CCNGG 2 cut(s) 57, 186
MunI CAATTG 1 cut(s) 308
Mva1269I GAATGC 1 cut(s) 272
MvaI CCWGG 1 cut(s) 57
NciI CCSGG 1 cut(s) 186
NdeII GATC 2 cut(s) 386, 397
NlaIII CATG 2 cut(s) 85, 385
NlaIV GGNNCC 2 cut(s) 54, 591
NmuCI GTSAC 1 cut(s) 69
NsbI TGCGCA 1 cut(s) 115
NsiI ATGCAT 1 cut(s) 274
NspI RCATGY 1 cut(s) 85
NspV TTCGAA 1 cut(s) 35
OliI CACNNNNGTG 1 cut(s) 76
PaeR7I CTCGAG 1 cut(s) 638
PceI AGGCCT 1 cut(s) 530
PctI GAATGC 1 cut(s) 272
PfeI GAWTC 3 cut(s) 8, 32, 715
PflFI GACNNNGTC 1 cut(s) 652
PinAI ACCGGT 1 cut(s) 437
PkrI GCNGC 2 cut(s) 90, 289
PmaCI CACGTG 1 cut(s) 224
PmlI CACGTG 1 cut(s) 224
Ppu21I YACGTR 1 cut(s) 224
PpuMI RGGWCCY 1 cut(s) 59
PshBI ATTAAT 1 cut(s) 699
Psp1406I AACGTT 1 cut(s) 509
Psp5II RGGWCCY 1 cut(s) 59
Psp6I CCWGG 1 cut(s) 55
PspCI CACGTG 1 cut(s) 224
PspFI CCCAGC 1 cut(s) 283
PspGI CCWGG 1 cut(s) 55
PspN4I GGNNCC 2 cut(s) 54, 591
PspPI GGNCC 3 cut(s) 59, 144, 518
PspPPI RGGWCCY 1 cut(s) 59
PstNI CAGNNNCTG 1 cut(s) 290
PsuI RGATCY 1 cut(s) 397
PsyI GACNNNGTC 1 cut(s) 652
PvuII CAGCTG 1 cut(s) 287
RsaI GTAC 1 cut(s) 380
RsaNI GTAC 1 cut(s) 379
RseI CAYNNNNRTG 1 cut(s) 76
SaqAI TTAA 1 cut(s) 699
SatI GCNGC 2 cut(s) 89, 288
Sau3AI GATC 2 cut(s) 386, 397
Sau96I GGNCC 3 cut(s) 59, 144, 518
ScrFI CCNGG 2 cut(s) 57, 186
SfaNI GCATC 1 cut(s) 88
SfcI CTRYAG 1 cut(s) 74
Sfr274I CTCGAG 1 cut(s) 638
SfuI TTCGAA 1 cut(s) 35
SgrAI CRCCGGYG 1 cut(s) 104
SinI GGWCC 3 cut(s) 59, 144, 518
SlaI CTCGAG 1 cut(s) 638
SmiMI CAYNNNNRTG 1 cut(s) 76
SmlI CTYRAG 3 cut(s) 15, 154, 638
SmoI CTYRAG 3 cut(s) 15, 154, 638
Sse9I AATT 1 cut(s) 308
SseBI AGGCCT 1 cut(s) 530
SsiI CCGC 3 cut(s) 65, 88, 108
SspI AATATT 1 cut(s) 697
SspMI CTAG 1 cut(s) 123
StuI AGGCCT 1 cut(s) 530
StyD4I CCNGG 2 cut(s) 55, 184
StyI CCWWGG 1 cut(s) 525
TaaI ACNGT 5 cut(s) 78, 196, 239, 451, 723
TaiI ACGT 6 cut(s) 153, 226, 247, 469, 512, 653
TaqI TCGA 3 cut(s) 6, 35, 639
TasI AATT 1 cut(s) 308
TatI WGTACW 1 cut(s) 378
TauI GCSGC 1 cut(s) 91
TfiI GAWTC 3 cut(s) 8, 32, 715
Tru1I TTAA 1 cut(s) 699
Tru9I TTAA 1 cut(s) 699
TscAI CASTG 4 cut(s) 83, 342, 396, 600
TseFI GTSAC 1 cut(s) 69
TseI GCWGC 1 cut(s) 287
Tsp45I GTSAC 1 cut(s) 69
TspDTI ATGAA 4 cut(s) 365, 378, 383, 387
TspGWI ACGGA 2 cut(s) 65, 312
TspRI CASTG 4 cut(s) 83, 342, 396, 600
Tth111I GACNNNGTC 1 cut(s) 652
VpaK11BI GGWCC 3 cut(s) 59, 144, 518
VspI ATTAAT 1 cut(s) 699
XceI RCATGY 1 cut(s) 85
XhoI CTCGAG 1 cut(s) 638
XmiI GTMKAC 2 cut(s) 241, 279
XspI CTAG 1 cut(s) 123
ZraI GACGTC 2 cut(s) 151, 651
Zsp2I ATGCAT 1 cut(s) 274
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.