Rh2BG486300

Pyridoxamine 5'-phosphate oxidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0008585
Physical Location & Seq
Reverse (-)
68628453 .. 68633376
4924 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG486300.1

Sequence Viewer

Length: 960 bp
ATGGAAGCCCTGGCAAGGTCTGTGTTCATACCCGAGACGTTTCCTCTTCATAACAACAACAACAACAACCCAGTTACTTCAATCAGAACACCCAGTAACCGAACTGGGTCTCTCAAAATCGGCCAAAGCTGCCATTTTTTCGGCCTCCGTGCCCAAGCTCTCTCCGGCGAGGCTTCTGGGTCCGGCGAGAATGAAGATGCAAGGCCTCTCAGTAGTGGCTTTGGCTTAGATAGTCTCTCTTTGTCTCAGGTAGTAATGTGTGGATGCATCAAATGGCTACTTCAGAGTTTTAATGACCTGTATTTTGTGATGAAGGGTAATTTAAATCACCGTGAAAGTGGTGAGAAGGATGTGGATGAGGCATTTAATGTAGACAGTTCTCCTACATCCCATTCTGCAAGTGGAGCAGGAGGAACAAGAGCTGGGCTCTTTAGGACTCCAATTTCTGGCGGGGTGCAGAGTGCAACTTCAGCTCATGGTTTACCTCGTCCGTCCCTAGCTGTACGCAATCTTATGGAGCAGGCCAGATTTGCTCATTTGTGCACTGTGATGTCACGCATGCACCATCGACGAGAAGGATATCCATTTGGTTCGCTGGTTGATTTTGCACCTGATTCAATGGGCCATCCTATCTTTTCGTTTTCCCCATTGGCCATCCACACACGGAATTTGTTGGCTGATCCAAGATGCACGCTTGTTGTGCAGATTCCTGGATGGAGCGGCTTGTCAAATGCAAGGGTAACCATTTTTGGTGATGTCTACCCACTTCCAGAGGATCAGCAGGAATGGGCTCATAAGCAATACATAGCAAAACATCAACAGGGGCCTTCTCAGCAATGGGGAAATTTCTACTACTTCAGGATGCAGAACATAAGGTTGGTTTTTGTTCTAGTAATTGCCAAATTGGTTGTATGTGGTTGTTTTTTTTACCTGACAATGCCTATTTTTTTATTAGAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pyrid_oxidase_2

Protein Analysis

319

Amino Acids

35.23

Weight (kDa)

8.18

Isoelectric Point (pI)

46.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CREG_beta-barrel PF13883 169 - 282 2.1e-10 CREG, beta-barrel
PNPOx_N PF01243 169 - 290 8.2e-07 Pyridoxamine 5'-phosphate oxidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 446
AccBSI CCGCTC 1 cut(s) 720
AccI GTMKAC 2 cut(s) 372, 759
AciI CCGC 2 cut(s) 450, 720
AclWI GGATC 2 cut(s) 674, 783
AcoI YGGCCR 2 cut(s) 121, 651
AcsI RAATTY 2 cut(s) 667, 844
AcuI CTGAAG 3 cut(s) 266, 453, 841
AfaI GTAC 1 cut(s) 504
AfiI CCNNNNNNNGG 2 cut(s) 15, 446
AgsI TTSAA 2 cut(s) 81, 618
AjnI CCWGG 2 cut(s) 9, 709
AjuI GAANNNNNNNTTGG 2 cut(s) 860, 892
AluBI AGCT 5 cut(s) 129, 158, 422, 473, 500
AluI AGCT 5 cut(s) 129, 158, 422, 473, 500
Alw21I GWGCWC 1 cut(s) 545
Alw26I GTCTC 4 cut(s) 29, 114, 239, 249
Alw44I GTGCAC 1 cut(s) 541
AlwI GGATC 2 cut(s) 674, 783
Ama87I CYCGRG 1 cut(s) 32
AoxI GGCC 7 cut(s) 121, 142, 203, 522, 622, 651, 824
ApaLI GTGCAC 1 cut(s) 541
ApeKI GCWGC 1 cut(s) 129
ApoI RAATTY 2 cut(s) 667, 844
ArsI GACNNNNNNTTYG 2 cut(s) 287, 319
AspS9I GGNCC 3 cut(s) 180, 622, 824
AsuHPI GGTGA 3 cut(s) 320, 353, 764
AvaI CYCGRG 1 cut(s) 32
AvaII GGWCC 1 cut(s) 180
BaeGI GKGCMC 2 cut(s) 154, 545
BalI TGGCCA 1 cut(s) 653
BanII GRGCYC 2 cut(s) 429, 793
Bbv12I GWGCWC 1 cut(s) 545
BbvI GCAGC 1 cut(s) 116
BccI CCATC 4 cut(s) 573, 633, 662, 708
BciT130I CCWGG 2 cut(s) 11, 711
BcoDI GTCTC 4 cut(s) 29, 114, 239, 249
BfaI CTAG 2 cut(s) 497, 890
BisI GCNGC 2 cut(s) 130, 721
BlsI GCNGC 2 cut(s) 131, 722
Bme1390I CCNGG 2 cut(s) 11, 711
Bme18I GGWCC 1 cut(s) 180
BmeT110I CYCGRG 1 cut(s) 32
BmgT120I GGNCC 3 cut(s) 180, 622, 824
BmiI GGNNCC 2 cut(s) 181, 825
BmrFI CCNGG 2 cut(s) 11, 711
BmrI ACTGGG 3 cut(s) 65, 87, 114
BmsI GCATC 5 cut(s) 187, 254, 276, 677, 852
BmuI ACTGGG 3 cut(s) 65, 87, 114
BplI GAGNNNNNCTC 2 cut(s) 411, 443
BsaI GGTCTC 1 cut(s) 114
BsaJI CCNNGG 1 cut(s) 9
Bsc4I CCNNNNNNNGG 2 cut(s) 15, 446
Bse1I ACTGG 3 cut(s) 71, 93, 109
Bse3DI GCAATG 1 cut(s) 842
BseBI CCWGG 2 cut(s) 11, 711
BseDI CCNNGG 1 cut(s) 9
BseGI GGATG 8 cut(s) 269, 355, 361, 386, 625, 654, 719, 867
BseLI CCNNNNNNNGG 2 cut(s) 15, 446
BseMI GCAATG 1 cut(s) 842
BseMII CTCAG 3 cut(s) 223, 260, 845
BseNI ACTGG 3 cut(s) 71, 93, 109
BseSI GKGCMC 2 cut(s) 154, 545
BseXI GCAGC 1 cut(s) 116
BseYI CCCAGC 1 cut(s) 422
BsgI GTGCAG 2 cut(s) 476, 722
BshFI GGCC 7 cut(s) 123, 144, 205, 524, 624, 653, 826
BsiHKAI GWGCWC 1 cut(s) 545
BsiHKCI CYCGRG 1 cut(s) 32
BsiSI CCGG 2 cut(s) 165, 183
BslFI GGGAC 1 cut(s) 478
BslI CCNNNNNNNGG 2 cut(s) 15, 446
BsmAI GTCTC 4 cut(s) 29, 114, 239, 249
BsmBI CGTCTC 1 cut(s) 29
BsmFI GGGAC 1 cut(s) 478
BsnI GGCC 7 cut(s) 123, 144, 205, 524, 624, 653, 826
Bso31I GGTCTC 1 cut(s) 114
BsoBI CYCGRG 1 cut(s) 32
Bsp1286I GDGCHC 4 cut(s) 154, 429, 545, 793
Bsp143I GATC 2 cut(s) 679, 775
BspACI CCGC 2 cut(s) 450, 720
BspANI GGCC 7 cut(s) 123, 144, 205, 524, 624, 653, 826
BspCNI CTCAG 3 cut(s) 222, 259, 844
BspLI GGNNCC 2 cut(s) 181, 825
BspPI GGATC 2 cut(s) 674, 783
BspTNI GGTCTC 1 cut(s) 114
BsrBI CCGCTC 1 cut(s) 720
BsrDI GCAATG 1 cut(s) 842
BsrI ACTGG 3 cut(s) 71, 93, 109
BssECI CCNNGG 1 cut(s) 9
BssMI GATC 2 cut(s) 679, 775
Bst2UI CCWGG 2 cut(s) 11, 711
Bst4CI ACNGT 3 cut(s) 332, 377, 547
Bst6I CTCTTC 1 cut(s) 51
BstC8I GCNNGC 3 cut(s) 522, 560, 692
BstDEI CTNAG 4 cut(s) 209, 226, 246, 831
BstEII GGTNACC 1 cut(s) 739
BstF5I GGATG 8 cut(s) 269, 355, 361, 386, 625, 654, 719, 867
BstKTI GATC 2 cut(s) 682, 778
BstMAI GTCTC 4 cut(s) 29, 114, 239, 249
BstMBI GATC 2 cut(s) 679, 775
BstMWI GCNNNNNNNGC 6 cut(s) 129, 404, 470, 530, 700, 832
BstNI CCWGG 2 cut(s) 11, 711
BstNSI RCATGY 1 cut(s) 562
BstPI GGTNACC 1 cut(s) 739
BstSCI CCNGG 2 cut(s) 9, 709
BstSLI GKGCMC 2 cut(s) 154, 545
BstV1I GCAGC 1 cut(s) 116
BsuRI GGCC 7 cut(s) 123, 144, 205, 524, 624, 653, 826
BtsCI GGATG 8 cut(s) 269, 355, 361, 386, 625, 654, 719, 867
BtsIMutI CAGTG 1 cut(s) 543
Cac8I GCNNGC 3 cut(s) 522, 560, 692
Cfr13I GGNCC 3 cut(s) 180, 622, 824
Csp6I GTAC 1 cut(s) 503
CviAII CATG 2 cut(s) 476, 559
CviQI GTAC 1 cut(s) 503
DdeI CTNAG 4 cut(s) 209, 226, 246, 831
DpnI GATC 2 cut(s) 681, 777
DpnII GATC 2 cut(s) 679, 775
DraI TTTAAA 1 cut(s) 324
EaeI YGGCCR 2 cut(s) 121, 651
Eam1104I CTCTTC 1 cut(s) 51
EarI CTCTTC 1 cut(s) 51
Eco147I AGGCCT 1 cut(s) 205
Eco24I GRGCYC 2 cut(s) 429, 793
Eco31I GGTCTC 1 cut(s) 114
Eco32I GATATC 1 cut(s) 581
Eco47I GGWCC 1 cut(s) 180
Eco57I CTGAAG 3 cut(s) 266, 453, 841
Eco88I CYCGRG 1 cut(s) 32
Eco91I GGTNACC 1 cut(s) 739
EcoO109I RGGNCCY 1 cut(s) 824
EcoO65I GGTNACC 1 cut(s) 739
EcoRII CCWGG 2 cut(s) 9, 709
EcoRV GATATC 1 cut(s) 581
EcoT22I ATGCAT 1 cut(s) 269
EcoT38I GRGCYC 2 cut(s) 429, 793
Esp3I CGTCTC 1 cut(s) 29
FaeI CATG 2 cut(s) 479, 562
FaiI YATR 9 cut(s) 29, 51, 477, 515, 560, 795, 806, 872, 913
FaqI GGGAC 1 cut(s) 478
FatI CATG 2 cut(s) 475, 558
FauI CCCGC 1 cut(s) 443
FblI GTMKAC 2 cut(s) 372, 759
Fnu4HI GCNGC 2 cut(s) 130, 721
FokI GGATG 8 cut(s) 276, 362, 368, 373, 612, 641, 726, 874
FriOI GRGCYC 2 cut(s) 429, 793
Fsp4HI GCNGC 2 cut(s) 130, 721
FspBI CTAG 2 cut(s) 497, 890
GluI GCNGC 2 cut(s) 130, 721
GsaI CCCAGC 1 cut(s) 426
HaeIII GGCC 7 cut(s) 123, 144, 205, 524, 624, 653, 826
HapII CCGG 2 cut(s) 165, 183
Hin1II CATG 2 cut(s) 479, 562
HinfI GANTC 3 cut(s) 436, 614, 706
HpaII CCGG 2 cut(s) 165, 183
HphI GGTGA 3 cut(s) 320, 353, 764
Hpy166II GTNNAC 4 cut(s) 373, 482, 543, 760
Hpy188I TCNGA 2 cut(s) 86, 285
Hpy188III TCNNGA 2 cut(s) 770, 859
Hpy8I GTNNAC 4 cut(s) 373, 482, 543, 760
Hpy99I CGWCG 1 cut(s) 573
HpyAV CCTTC 4 cut(s) 307, 340, 569, 837
HpyCH4III ACNGT 3 cut(s) 332, 377, 547
HpyCH4IV ACGT 1 cut(s) 38
HpyF10VI GCNNNNNNNGC 6 cut(s) 129, 404, 470, 530, 700, 832
HpyF3I CTNAG 4 cut(s) 209, 226, 246, 831
HpySE526I ACGT 1 cut(s) 38
Hsp92II CATG 2 cut(s) 479, 562
Kzo9I GATC 2 cut(s) 679, 775
LmnI GCTCC 3 cut(s) 404, 517, 717
Lsp1109I GCAGC 1 cut(s) 116
LweI GCATC 5 cut(s) 187, 254, 276, 677, 852
MaeI CTAG 2 cut(s) 497, 890
MaeII ACGT 1 cut(s) 38
MaeIII GTNAC 4 cut(s) 73, 95, 552, 739
MalI GATC 2 cut(s) 681, 777
MbiI CCGCTC 1 cut(s) 720
MboI GATC 2 cut(s) 679, 775
MboII GAAGA 2 cut(s) 38, 206
MhlI GDGCHC 4 cut(s) 154, 429, 545, 793
MlsI TGGCCA 1 cut(s) 653
MluCI AATT 6 cut(s) 319, 441, 667, 844, 894, 902
MluNI TGGCCA 1 cut(s) 653
MlyI GAGTC 1 cut(s) 430
MnlI CCTC 8 cut(s) 54, 155, 163, 216, 352, 404, 495, 766
Mox20I TGGCCA 1 cut(s) 653
Mph1103I ATGCAT 1 cut(s) 269
MscI TGGCCA 1 cut(s) 653
MseI TTAA 3 cut(s) 291, 323, 366
MslI CAYNNNNRTG 1 cut(s) 548
Msp20I TGGCCA 1 cut(s) 653
MspI CCGG 2 cut(s) 165, 183
MspR9I CCNGG 2 cut(s) 11, 711
MvaI CCWGG 2 cut(s) 11, 711
MwoI GCNNNNNNNGC 6 cut(s) 129, 404, 470, 530, 700, 832
NdeII GATC 2 cut(s) 679, 775
NlaIII CATG 2 cut(s) 479, 562
NlaIV GGNNCC 2 cut(s) 181, 825
NmuCI GTSAC 1 cut(s) 552
NsiI ATGCAT 1 cut(s) 269
NspI RCATGY 1 cut(s) 562
PaeI GCATGC 1 cut(s) 562
PceI AGGCCT 1 cut(s) 205
PfeI GAWTC 2 cut(s) 614, 706
PflMI CCANNNNNTGG 1 cut(s) 446
PfoI TCCNGGA 1 cut(s) 709
PkrI GCNGC 2 cut(s) 131, 722
PleI GAGTC 1 cut(s) 430
PpsI GAGTC 1 cut(s) 430
Psp6I CCWGG 2 cut(s) 9, 709
PspEI GGTNACC 1 cut(s) 739
PspFI CCCAGC 1 cut(s) 422
PspGI CCWGG 2 cut(s) 9, 709
PspN4I GGNNCC 2 cut(s) 181, 825
PspPI GGNCC 3 cut(s) 180, 622, 824
RsaI GTAC 1 cut(s) 504
RsaNI GTAC 1 cut(s) 503
RseI CAYNNNNRTG 1 cut(s) 548
SaqAI TTAA 3 cut(s) 291, 323, 366
SatI GCNGC 2 cut(s) 130, 721
Sau3AI GATC 2 cut(s) 679, 775
Sau96I GGNCC 3 cut(s) 180, 622, 824
SchI GAGTC 1 cut(s) 430
ScrFI CCNGG 2 cut(s) 11, 711
SduI GDGCHC 4 cut(s) 154, 429, 545, 793
SfaNI GCATC 5 cut(s) 187, 254, 276, 677, 852
SinI GGWCC 1 cut(s) 180
SmiI ATTTAAAT 1 cut(s) 324
SmiMI CAYNNNNRTG 1 cut(s) 548
SphI GCATGC 1 cut(s) 562
Sse9I AATT 6 cut(s) 319, 441, 667, 844, 894, 902
SseBI AGGCCT 1 cut(s) 205
SsiI CCGC 2 cut(s) 450, 720
SspMI CTAG 2 cut(s) 497, 890
StuI AGGCCT 1 cut(s) 205
StyD4I CCNGG 2 cut(s) 9, 709
SwaI ATTTAAAT 1 cut(s) 324
TaaI ACNGT 3 cut(s) 332, 377, 547
TaiI ACGT 1 cut(s) 41
TaqI TCGA 1 cut(s) 568
TasI AATT 6 cut(s) 319, 441, 667, 844, 894, 902
TauI GCSGC 1 cut(s) 723
TfiI GAWTC 2 cut(s) 614, 706
Tru1I TTAA 3 cut(s) 291, 323, 366
Tru9I TTAA 3 cut(s) 291, 323, 366
TscAI CASTG 1 cut(s) 550
TseFI GTSAC 1 cut(s) 552
TseI GCWGC 1 cut(s) 129
Tsp45I GTSAC 1 cut(s) 552
TspDTI ATGAA 4 cut(s) 16, 38, 207, 326
TspGWI ACGGA 3 cut(s) 137, 480, 679
TspRI CASTG 1 cut(s) 550
Van91I CCANNNNNTGG 1 cut(s) 446
VneI GTGCAC 1 cut(s) 541
VpaK11BI GGWCC 1 cut(s) 180
XapI RAATTY 2 cut(s) 667, 844
XceI RCATGY 1 cut(s) 562
XcmI CCANNNNNNNNNTGG 1 cut(s) 398
XmiI GTMKAC 2 cut(s) 372, 759
XspI CTAG 2 cut(s) 497, 890
Zsp2I ATGCAT 1 cut(s) 269
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.