Rh2DG495600

Pyridoxamine 5'-phosphate oxidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0008585
Physical Location & Seq
Reverse (-)
71552182 .. 71557105
4924 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG495600.1

Sequence Viewer

Length: 1224 bp
ATGGAAGCCCTGGCAAGGTCTGTGTTCATACCCGAGACGTTTCCTCTTCATAACAACAACAACAACAACCCAGTTACTTCAATCAGAACACCCAGTAACCGAACTGGGTCTCTCAAAATCGGCCAAAGCTGCCATTTTTTCGGCCTCCGTGCCCAAGCTCTCTCCGGCGAGGCTTCTGGGTCCGGCGAGAATGAAGATGCAAGGCCTCTCAGTAGTGGCTTTGGCTTAGATAGTCTCTCTTTGTCTCAGGTAGTAATGTGTGGATGCATCAAATGGCTACTTCAGAGTTTTAATGACCTGTATTTTGTGATGAAGGGTAATTTAAATCACCGTGAAAGTGGTGAGAAGGATGTGGATGAGGCATTTAATGTAGACAGTTCTCCTACATCCCATTCTGCAAGTGGAGCAGGAGGAACAAGAGCTGGGCTCTTTAGGACTCCAATTTCTGGCGGGGTGCAGAGTGCAACTTCAGCTCATGGTTTACCTCGTCCGTCCCTAGCTGTACGCAATCTTATGGAGCAGGCCAGATTTGCTCATTTGTGCACTGTGATGTCACGCATGCACCATCGACGAGAAGGATATCCATTTGGTTCGCTGGTTGATTTTGCACCTGATTCAATGGGCCATCCTATCTTTTCGTTTTCCCCATTGGCCATCCACACACGGAATTTGTTGGCTGATCCAAGATGCACGCTTGTTGTGCAGATTCCTGGATGGAGCGGCTTGTCAAATGCAAGGGTAACCATTTTTGGTGATGTCTACCCACTTCCAGAGGATCAGCAGGAATGGGCTCATAAGCAATACATAGCAAAACATCAACAGGGGCCTTCTCAGCAATGGGGAAATTTCTACTACTTCAGGATGCAGAACATAAGTGACATTTATTTCATTGGTGGCTTTGGGACTGTCGCTTGGGTTGATGTGAAAGAGTACGAGGCCCTTCAGCCTGATAAGATTGCAGTTGATGGAGGGGAGCAAAACCTGAAGGAACTTAATGCAATCTTTTCTAAGCCCCTAAAAGAGCTACTGTCCCTTGAATCTGAGGTGGATGATGCTGCTTTCATCTCTATAGACAGCAAAGGAACGGATATTCGGGTCCGTCAGGGAGCACAGTTCAACATTCAGAGGCTATCATTTGAAGAAGGCCATTCTGTTGAGACATTAGAGGAAGCCAAGGCTGCTCTTAGGAAACTTATAAACAAAGGCCGAGTGCATAGTTTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pyrid_oxidase_2

Protein Analysis

407

Amino Acids

44.84

Weight (kDa)

6.28

Isoelectric Point (pI)

40.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CREG_beta-barrel PF13883 169 - 312 2.9e-18 CREG, beta-barrel
PNPOx_N PF01243 169 - 278 2.3e-06 Pyridoxamine 5'-phosphate oxidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1196
AccB7I CCANNNNNTGG 1 cut(s) 446
AccBSI CCGCTC 1 cut(s) 720
AccI GTMKAC 2 cut(s) 372, 759
AciI CCGC 2 cut(s) 450, 720
AclWI GGATC 2 cut(s) 674, 783
AcoI YGGCCR 2 cut(s) 121, 651
AcsI RAATTY 2 cut(s) 667, 844
AcuI CTGAAG 5 cut(s) 266, 453, 841, 926, 1004
AfaI GTAC 2 cut(s) 504, 932
AfiI CCNNNNNNNGG 2 cut(s) 15, 446
AgsI TTSAA 5 cut(s) 81, 618, 1037, 1117, 1139
AjnI CCWGG 2 cut(s) 9, 709
AluBI AGCT 6 cut(s) 129, 158, 422, 473, 500, 1024
AluI AGCT 6 cut(s) 129, 158, 422, 473, 500, 1024
Alw21I GWGCWC 2 cut(s) 545, 1111
Alw26I GTCTC 5 cut(s) 29, 114, 239, 249, 1151
Alw44I GTGCAC 1 cut(s) 541
AlwI GGATC 2 cut(s) 674, 783
Ama87I CYCGRG 1 cut(s) 32
ApaLI GTGCAC 1 cut(s) 541
ApeKI GCWGC 3 cut(s) 129, 1055, 1178
ApoI RAATTY 2 cut(s) 667, 844
ArsI GACNNNNNNTTYG 2 cut(s) 287, 319
AspS9I GGNCC 5 cut(s) 180, 622, 824, 937, 1096
AsuHPI GGTGA 3 cut(s) 320, 353, 764
AvaI CYCGRG 1 cut(s) 32
AvaII GGWCC 2 cut(s) 180, 1096
BaeGI GKGCMC 2 cut(s) 154, 545
BalI TGGCCA 1 cut(s) 653
BanII GRGCYC 2 cut(s) 429, 793
Bbv12I GWGCWC 2 cut(s) 545, 1111
BbvI GCAGC 3 cut(s) 116, 1042, 1165
BccI CCATC 5 cut(s) 573, 633, 662, 708, 959
BciT130I CCWGG 2 cut(s) 11, 711
BcoDI GTCTC 5 cut(s) 29, 114, 239, 249, 1151
BfaI CTAG 1 cut(s) 497
BfmI CTRYAG 1 cut(s) 1068
BisI GCNGC 4 cut(s) 130, 721, 1056, 1179
BlsI GCNGC 4 cut(s) 131, 722, 1057, 1180
Bme1390I CCNGG 2 cut(s) 11, 711
Bme18I GGWCC 2 cut(s) 180, 1096
BmeT110I CYCGRG 1 cut(s) 32
BmgT120I GGNCC 5 cut(s) 180, 622, 824, 937, 1096
BmiI GGNNCC 3 cut(s) 181, 825, 1097
BmrFI CCNGG 2 cut(s) 11, 711
BmrI ACTGGG 3 cut(s) 65, 87, 114
BmsI GCATC 6 cut(s) 187, 254, 276, 677, 852, 1042
BmuI ACTGGG 3 cut(s) 65, 87, 114
BplI GAGNNNNNCTC 2 cut(s) 411, 443
BsaI GGTCTC 1 cut(s) 114
BsaJI CCNNGG 2 cut(s) 9, 1173
Bsc4I CCNNNNNNNGG 2 cut(s) 15, 446
Bse1I ACTGG 3 cut(s) 71, 93, 109
Bse3DI GCAATG 1 cut(s) 842
BseBI CCWGG 2 cut(s) 11, 711
BseDI CCNNGG 2 cut(s) 9, 1173
BseGI GGATG 9 cut(s) 269, 355, 361, 386, 625, 654, 719, 867, 1054
BseLI CCNNNNNNNGG 2 cut(s) 15, 446
BseMI GCAATG 1 cut(s) 842
BseMII CTCAG 4 cut(s) 223, 260, 845, 1032
BseNI ACTGG 3 cut(s) 71, 93, 109
BseSI GKGCMC 2 cut(s) 154, 545
BseXI GCAGC 3 cut(s) 116, 1042, 1165
BseYI CCCAGC 1 cut(s) 422
BsgI GTGCAG 2 cut(s) 476, 722
BsiHKAI GWGCWC 2 cut(s) 545, 1111
BsiHKCI CYCGRG 1 cut(s) 32
BsiSI CCGG 2 cut(s) 165, 183
BslFI GGGAC 3 cut(s) 478, 916, 1015
BslI CCNNNNNNNGG 2 cut(s) 15, 446
BsmAI GTCTC 5 cut(s) 29, 114, 239, 249, 1151
BsmBI CGTCTC 1 cut(s) 29
BsmFI GGGAC 3 cut(s) 478, 916, 1015
Bso31I GGTCTC 1 cut(s) 114
BsoBI CYCGRG 1 cut(s) 32
Bsp1286I GDGCHC 5 cut(s) 154, 429, 545, 793, 1111
Bsp143I GATC 2 cut(s) 679, 775
BspACI CCGC 2 cut(s) 450, 720
BspCNI CTCAG 4 cut(s) 222, 259, 844, 1033
BspLI GGNNCC 3 cut(s) 181, 825, 1097
BspPI GGATC 2 cut(s) 674, 783
BspTNI GGTCTC 1 cut(s) 114
BsrBI CCGCTC 1 cut(s) 720
BsrDI GCAATG 1 cut(s) 842
BsrI ACTGG 3 cut(s) 71, 93, 109
BssECI CCNNGG 2 cut(s) 9, 1173
BssMI GATC 2 cut(s) 679, 775
BssT1I CCWWGG 1 cut(s) 1173
Bst2UI CCWGG 2 cut(s) 11, 711
Bst4CI ACNGT 6 cut(s) 332, 377, 547, 907, 1029, 1113
Bst6I CTCTTC 1 cut(s) 51
BstC8I GCNNGC 3 cut(s) 522, 560, 692
BstDEI CTNAG 7 cut(s) 209, 226, 246, 831, 1008, 1041, 1184
BstEII GGTNACC 1 cut(s) 739
BstF5I GGATG 9 cut(s) 269, 355, 361, 386, 625, 654, 719, 867, 1054
BstKTI GATC 2 cut(s) 682, 778
BstMAI GTCTC 5 cut(s) 29, 114, 239, 249, 1151
BstMBI GATC 2 cut(s) 679, 775
BstMWI GCNNNNNNNGC 7 cut(s) 129, 404, 470, 530, 700, 832, 1178
BstNI CCWGG 2 cut(s) 11, 711
BstNSI RCATGY 1 cut(s) 562
BstPI GGTNACC 1 cut(s) 739
BstSCI CCNGG 2 cut(s) 9, 709
BstSFI CTRYAG 1 cut(s) 1068
BstSLI GKGCMC 2 cut(s) 154, 545
BstV1I GCAGC 3 cut(s) 116, 1042, 1165
BtsCI GGATG 9 cut(s) 269, 355, 361, 386, 625, 654, 719, 867, 1054
BtsIMutI CAGTG 1 cut(s) 543
Cac8I GCNNGC 3 cut(s) 522, 560, 692
Cfr13I GGNCC 5 cut(s) 180, 622, 824, 937, 1096
Csp6I GTAC 2 cut(s) 503, 931
CviAII CATG 2 cut(s) 476, 559
CviQI GTAC 2 cut(s) 503, 931
DdeI CTNAG 7 cut(s) 209, 226, 246, 831, 1008, 1041, 1184
DpnI GATC 2 cut(s) 681, 777
DpnII GATC 2 cut(s) 679, 775
DraI TTTAAA 1 cut(s) 324
EaeI YGGCCR 2 cut(s) 121, 651
Eam1104I CTCTTC 1 cut(s) 51
EarI CTCTTC 1 cut(s) 51
Eco130I CCWWGG 1 cut(s) 1173
Eco147I AGGCCT 1 cut(s) 205
Eco24I GRGCYC 2 cut(s) 429, 793
Eco31I GGTCTC 1 cut(s) 114
Eco32I GATATC 1 cut(s) 581
Eco47I GGWCC 2 cut(s) 180, 1096
Eco57I CTGAAG 5 cut(s) 266, 453, 841, 926, 1004
Eco88I CYCGRG 1 cut(s) 32
Eco91I GGTNACC 1 cut(s) 739
EcoO109I RGGNCCY 2 cut(s) 824, 937
EcoO65I GGTNACC 1 cut(s) 739
EcoRII CCWGG 2 cut(s) 9, 709
EcoRV GATATC 1 cut(s) 581
EcoT14I CCWWGG 1 cut(s) 1173
EcoT22I ATGCAT 1 cut(s) 269
EcoT38I GRGCYC 2 cut(s) 429, 793
ErhI CCWWGG 1 cut(s) 1173
Esp3I CGTCTC 1 cut(s) 29
FaeI CATG 2 cut(s) 479, 562
FaqI GGGAC 3 cut(s) 478, 916, 1015
FatI CATG 2 cut(s) 475, 558
FauI CCCGC 1 cut(s) 443
FblI GTMKAC 2 cut(s) 372, 759
Fnu4HI GCNGC 4 cut(s) 130, 721, 1056, 1179
FokI GGATG 9 cut(s) 276, 362, 368, 373, 612, 641, 726, 874, 1061
FriOI GRGCYC 2 cut(s) 429, 793
Fsp4HI GCNGC 4 cut(s) 130, 721, 1056, 1179
FspBI CTAG 1 cut(s) 497
GluI GCNGC 4 cut(s) 130, 721, 1056, 1179
GsaI CCCAGC 1 cut(s) 426
HapII CCGG 2 cut(s) 165, 183
Hin1II CATG 2 cut(s) 479, 562
HinfI GANTC 4 cut(s) 436, 614, 706, 1037
HpaII CCGG 2 cut(s) 165, 183
HphI GGTGA 3 cut(s) 320, 353, 764
Hpy166II GTNNAC 4 cut(s) 373, 482, 543, 760
Hpy188I TCNGA 4 cut(s) 86, 285, 1042, 1125
Hpy188III TCNNGA 2 cut(s) 770, 859
Hpy8I GTNNAC 4 cut(s) 373, 482, 543, 760
Hpy99I CGWCG 1 cut(s) 573
HpyAV CCTTC 7 cut(s) 307, 340, 569, 837, 950, 979, 1136
HpyCH4III ACNGT 6 cut(s) 332, 377, 547, 907, 1029, 1113
HpyCH4IV ACGT 1 cut(s) 38
HpyF10VI GCNNNNNNNGC 7 cut(s) 129, 404, 470, 530, 700, 832, 1178
HpyF3I CTNAG 7 cut(s) 209, 226, 246, 831, 1008, 1041, 1184
HpySE526I ACGT 1 cut(s) 38
Hsp92II CATG 2 cut(s) 479, 562
Kzo9I GATC 2 cut(s) 679, 775
LmnI GCTCC 5 cut(s) 404, 517, 717, 973, 1106
Lsp1109I GCAGC 3 cut(s) 116, 1042, 1165
LweI GCATC 6 cut(s) 187, 254, 276, 677, 852, 1042
MaeI CTAG 1 cut(s) 497
MaeII ACGT 1 cut(s) 38
MaeIII GTNAC 5 cut(s) 73, 95, 552, 739, 875
MalI GATC 2 cut(s) 681, 777
MbiI CCGCTC 1 cut(s) 720
MboI GATC 2 cut(s) 679, 775
MboII GAAGA 3 cut(s) 38, 206, 1151
MhlI GDGCHC 5 cut(s) 154, 429, 545, 793, 1111
MlsI TGGCCA 1 cut(s) 653
MluCI AATT 4 cut(s) 319, 441, 667, 844
MluNI TGGCCA 1 cut(s) 653
MlyI GAGTC 1 cut(s) 430
Mox20I TGGCCA 1 cut(s) 653
Mph1103I ATGCAT 1 cut(s) 269
MscI TGGCCA 1 cut(s) 653
MseI TTAA 4 cut(s) 291, 323, 366, 993
MslI CAYNNNNRTG 1 cut(s) 548
Msp20I TGGCCA 1 cut(s) 653
MspI CCGG 2 cut(s) 165, 183
MspR9I CCNGG 2 cut(s) 11, 711
MvaI CCWGG 2 cut(s) 11, 711
MwoI GCNNNNNNNGC 7 cut(s) 129, 404, 470, 530, 700, 832, 1178
NdeII GATC 2 cut(s) 679, 775
NlaIII CATG 2 cut(s) 479, 562
NlaIV GGNNCC 3 cut(s) 181, 825, 1097
NmuCI GTSAC 2 cut(s) 552, 875
NsiI ATGCAT 1 cut(s) 269
NspI RCATGY 1 cut(s) 562
PaeI GCATGC 1 cut(s) 562
PceI AGGCCT 1 cut(s) 205
PfeI GAWTC 3 cut(s) 614, 706, 1037
PflMI CCANNNNNTGG 1 cut(s) 446
PfoI TCCNGGA 1 cut(s) 709
PkrI GCNGC 4 cut(s) 131, 722, 1057, 1180
PleI GAGTC 1 cut(s) 430
PpsI GAGTC 1 cut(s) 430
PsiI TTATAA 1 cut(s) 1196
Psp6I CCWGG 2 cut(s) 9, 709
PspEI GGTNACC 1 cut(s) 739
PspFI CCCAGC 1 cut(s) 422
PspGI CCWGG 2 cut(s) 9, 709
PspN4I GGNNCC 3 cut(s) 181, 825, 1097
PspPI GGNCC 5 cut(s) 180, 622, 824, 937, 1096
RsaI GTAC 2 cut(s) 504, 932
RsaNI GTAC 2 cut(s) 503, 931
RseI CAYNNNNRTG 1 cut(s) 548
SaqAI TTAA 4 cut(s) 291, 323, 366, 993
SatI GCNGC 4 cut(s) 130, 721, 1056, 1179
Sau3AI GATC 2 cut(s) 679, 775
Sau96I GGNCC 5 cut(s) 180, 622, 824, 937, 1096
SchI GAGTC 1 cut(s) 430
ScrFI CCNGG 2 cut(s) 11, 711
SduI GDGCHC 5 cut(s) 154, 429, 545, 793, 1111
SfaNI GCATC 6 cut(s) 187, 254, 276, 677, 852, 1042
SfcI CTRYAG 1 cut(s) 1068
SinI GGWCC 2 cut(s) 180, 1096
SmiI ATTTAAAT 1 cut(s) 324
SmiMI CAYNNNNRTG 1 cut(s) 548
SphI GCATGC 1 cut(s) 562
Sse9I AATT 4 cut(s) 319, 441, 667, 844
SseBI AGGCCT 1 cut(s) 205
SsiI CCGC 2 cut(s) 450, 720
SspMI CTAG 1 cut(s) 497
StuI AGGCCT 1 cut(s) 205
StyD4I CCNGG 2 cut(s) 9, 709
StyI CCWWGG 1 cut(s) 1173
SwaI ATTTAAAT 1 cut(s) 324
TaaI ACNGT 6 cut(s) 332, 377, 547, 907, 1029, 1113
TaiI ACGT 1 cut(s) 41
TaqI TCGA 1 cut(s) 568
TasI AATT 4 cut(s) 319, 441, 667, 844
TauI GCSGC 1 cut(s) 723
TfiI GAWTC 3 cut(s) 614, 706, 1037
Tru1I TTAA 4 cut(s) 291, 323, 366, 993
Tru9I TTAA 4 cut(s) 291, 323, 366, 993
TscAI CASTG 1 cut(s) 550
TseFI GTSAC 2 cut(s) 552, 875
TseI GCWGC 3 cut(s) 129, 1055, 1178
Tsp45I GTSAC 2 cut(s) 552, 875
TspDTI ATGAA 6 cut(s) 16, 38, 207, 326, 877, 1051
TspGWI ACGGA 5 cut(s) 137, 480, 679, 1088, 1100
TspRI CASTG 1 cut(s) 550
Van91I CCANNNNNTGG 1 cut(s) 446
VneI GTGCAC 1 cut(s) 541
VpaK11BI GGWCC 2 cut(s) 180, 1096
XapI RAATTY 2 cut(s) 667, 844
XceI RCATGY 1 cut(s) 562
XcmI CCANNNNNNNNNTGG 1 cut(s) 398
XmiI GTMKAC 2 cut(s) 372, 759
XspI CTAG 1 cut(s) 497
Zsp2I ATGCAT 1 cut(s) 269
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.