Rh2CG460300

Pyridoxamine 5'-phosphate oxidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2C
OG0008585
Physical Location & Seq
Reverse (-)
62322575 .. 62326981
4407 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2CG460300.1

Sequence Viewer

Length: 963 bp
ATGGAAGCCCTGGCAAGCTCTGTGTTCATACCCGAGACGTTTCCACTTCATAACAACAACAACAACAACAACCCAGTTACTTCAATCAGAACACCCAATAACCGAACTGGGTCTCTGAAAATCGGCCAAAGCTGCCATTTTTTCGGCCTCCGTGCCCAAGCTCTCTCCGGCGAGGCTTCTGGGTCCGGCGAGAATGAAGATGCAAGGCCTCTCAGTAGTGGCTTTGGCTTAGATAGTCTCTCTTTGTCTCAGGTAGTAATGTGTGGATGCATCAAATGGCTACTTCAGAGTTTTAATGACCTGTATTTTGTGATGAAGGGTAATTTAAATCACCGTGAAAGTGGTGAGAAGGATGTGGATGAGGCATTTAATGTAGACAGTTCTCTTACATCCCATTCTGCAAGTGGAGCAGGAGGAACAAGAGCTGGGCTCTTTAGGACTCCGATTTCTGGCGGGGTGCAGAGTGCAACTTCAGCTCATGGTTTACCTCGTCCGTCCCTAGCTGTACGCAATCTTATGGAGCAGGCCAGATTTGCTCATTTGTGCACTGTGATGTCACGCATGCACCATCGACGAGAAGGATATCCATTTGGTTCGCTGGTTGATTTTGCACCTGATTCAATGGGCCATCCTATCTTTTCGTTTTCCCCATTGGCCATCCACACACGGAATTTGTTGGCTGATCCAAGATGCACGCTTGTTGTGCAGATTCCTGGATGGAGCGGCTTGTCAAATGCAAGGGTAACCATTTTTGGTGATGTCTACCCACTTCCAGAGGATCAGCAGGAATGGGCTCATAAGCAATACATAGCAAAACATCAACAGGGGCCTTCTCAGCAATGGGGAAATTTCTACTACTTCAGGATGCAGAACATAAGGTTGGTTTTTGTTCTAGTAATTGCCAAATTGGTTGTATGTGTTTGTTTTTTTTACCTGACAATGCCTATTTTTTTATTAGAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pyrid_oxidase_2

Protein Analysis

320

Amino Acids

35.36

Weight (kDa)

7.7

Isoelectric Point (pI)

42.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CREG_beta-barrel PF13883 170 - 281 2.7e-10 CREG, beta-barrel
PNPOx_N PF01243 170 - 291 8.3e-07 Pyridoxamine 5'-phosphate oxidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 723
AccI GTMKAC 2 cut(s) 375, 762
AciI CCGC 2 cut(s) 453, 723
AclWI GGATC 2 cut(s) 677, 786
AcoI YGGCCR 2 cut(s) 124, 654
AcsI RAATTY 2 cut(s) 670, 847
AcuI CTGAAG 3 cut(s) 269, 456, 844
AfaI GTAC 1 cut(s) 507
AfiI CCNNNNNNNGG 1 cut(s) 449
AgsI TTSAA 2 cut(s) 84, 621
AjnI CCWGG 2 cut(s) 9, 712
AjuI GAANNNNNNNTTGG 2 cut(s) 863, 895
AluBI AGCT 6 cut(s) 18, 132, 161, 425, 476, 503
AluI AGCT 6 cut(s) 18, 132, 161, 425, 476, 503
Alw21I GWGCWC 1 cut(s) 548
Alw26I GTCTC 4 cut(s) 29, 117, 242, 252
Alw44I GTGCAC 1 cut(s) 544
AlwI GGATC 2 cut(s) 677, 786
Ama87I CYCGRG 1 cut(s) 32
AoxI GGCC 7 cut(s) 124, 145, 206, 525, 625, 654, 827
ApaLI GTGCAC 1 cut(s) 544
ApeKI GCWGC 1 cut(s) 132
ApoI RAATTY 2 cut(s) 670, 847
ArsI GACNNNNNNTTYG 2 cut(s) 290, 322
AspS9I GGNCC 3 cut(s) 183, 625, 827
AsuHPI GGTGA 3 cut(s) 323, 356, 767
AvaI CYCGRG 1 cut(s) 32
AvaII GGWCC 1 cut(s) 183
BaeGI GKGCMC 2 cut(s) 157, 548
BalI TGGCCA 1 cut(s) 656
BanII GRGCYC 2 cut(s) 432, 796
Bbv12I GWGCWC 1 cut(s) 548
BbvI GCAGC 1 cut(s) 119
BccI CCATC 4 cut(s) 576, 636, 665, 711
BciT130I CCWGG 2 cut(s) 11, 714
BcoDI GTCTC 4 cut(s) 29, 117, 242, 252
BfaI CTAG 2 cut(s) 500, 893
BisI GCNGC 2 cut(s) 133, 724
BlsI GCNGC 2 cut(s) 134, 725
Bme1390I CCNGG 2 cut(s) 11, 714
Bme18I GGWCC 1 cut(s) 183
BmeT110I CYCGRG 1 cut(s) 32
BmgT120I GGNCC 3 cut(s) 183, 625, 827
BmiI GGNNCC 2 cut(s) 184, 828
BmrFI CCNGG 2 cut(s) 11, 714
BmrI ACTGGG 2 cut(s) 68, 117
BmsI GCATC 5 cut(s) 190, 257, 279, 680, 855
BmuI ACTGGG 2 cut(s) 68, 117
BplI GAGNNNNNCTC 2 cut(s) 414, 446
BsaI GGTCTC 1 cut(s) 117
BsaJI CCNNGG 1 cut(s) 9
Bsc4I CCNNNNNNNGG 1 cut(s) 449
Bse1I ACTGG 2 cut(s) 74, 112
Bse3DI GCAATG 1 cut(s) 845
BseBI CCWGG 2 cut(s) 11, 714
BseDI CCNNGG 1 cut(s) 9
BseGI GGATG 8 cut(s) 272, 358, 364, 389, 628, 657, 722, 870
BseLI CCNNNNNNNGG 1 cut(s) 449
BseMI GCAATG 1 cut(s) 845
BseMII CTCAG 3 cut(s) 226, 263, 848
BseNI ACTGG 2 cut(s) 74, 112
BseSI GKGCMC 2 cut(s) 157, 548
BseXI GCAGC 1 cut(s) 119
BseYI CCCAGC 1 cut(s) 425
BsgI GTGCAG 2 cut(s) 479, 725
BshFI GGCC 7 cut(s) 126, 147, 208, 527, 627, 656, 829
BsiHKAI GWGCWC 1 cut(s) 548
BsiHKCI CYCGRG 1 cut(s) 32
BsiSI CCGG 2 cut(s) 168, 186
BslFI GGGAC 1 cut(s) 481
BslI CCNNNNNNNGG 1 cut(s) 449
BsmAI GTCTC 4 cut(s) 29, 117, 242, 252
BsmBI CGTCTC 1 cut(s) 29
BsmFI GGGAC 1 cut(s) 481
BsnI GGCC 7 cut(s) 126, 147, 208, 527, 627, 656, 829
Bso31I GGTCTC 1 cut(s) 117
BsoBI CYCGRG 1 cut(s) 32
Bsp1286I GDGCHC 4 cut(s) 157, 432, 548, 796
Bsp143I GATC 2 cut(s) 682, 778
BspACI CCGC 2 cut(s) 453, 723
BspANI GGCC 7 cut(s) 126, 147, 208, 527, 627, 656, 829
BspCNI CTCAG 3 cut(s) 225, 262, 847
BspLI GGNNCC 2 cut(s) 184, 828
BspPI GGATC 2 cut(s) 677, 786
BspTNI GGTCTC 1 cut(s) 117
BsrBI CCGCTC 1 cut(s) 723
BsrDI GCAATG 1 cut(s) 845
BsrI ACTGG 2 cut(s) 74, 112
BssECI CCNNGG 1 cut(s) 9
BssMI GATC 2 cut(s) 682, 778
Bst2UI CCWGG 2 cut(s) 11, 714
Bst4CI ACNGT 3 cut(s) 335, 380, 550
BstC8I GCNNGC 4 cut(s) 16, 525, 563, 695
BstDEI CTNAG 4 cut(s) 212, 229, 249, 834
BstEII GGTNACC 1 cut(s) 742
BstF5I GGATG 8 cut(s) 272, 358, 364, 389, 628, 657, 722, 870
BstKTI GATC 2 cut(s) 685, 781
BstMAI GTCTC 4 cut(s) 29, 117, 242, 252
BstMBI GATC 2 cut(s) 682, 778
BstMWI GCNNNNNNNGC 6 cut(s) 132, 407, 473, 533, 703, 835
BstNI CCWGG 2 cut(s) 11, 714
BstNSI RCATGY 1 cut(s) 565
BstPI GGTNACC 1 cut(s) 742
BstSCI CCNGG 2 cut(s) 9, 712
BstSLI GKGCMC 2 cut(s) 157, 548
BstV1I GCAGC 1 cut(s) 119
BsuRI GGCC 7 cut(s) 126, 147, 208, 527, 627, 656, 829
BtsCI GGATG 8 cut(s) 272, 358, 364, 389, 628, 657, 722, 870
BtsIMutI CAGTG 1 cut(s) 546
Cac8I GCNNGC 4 cut(s) 16, 525, 563, 695
Cfr13I GGNCC 3 cut(s) 183, 625, 827
Csp6I GTAC 1 cut(s) 506
CviAII CATG 2 cut(s) 479, 562
CviQI GTAC 1 cut(s) 506
DdeI CTNAG 4 cut(s) 212, 229, 249, 834
DpnI GATC 2 cut(s) 684, 780
DpnII GATC 2 cut(s) 682, 778
DraI TTTAAA 1 cut(s) 327
EaeI YGGCCR 2 cut(s) 124, 654
Eco147I AGGCCT 1 cut(s) 208
Eco24I GRGCYC 2 cut(s) 432, 796
Eco31I GGTCTC 1 cut(s) 117
Eco32I GATATC 1 cut(s) 584
Eco47I GGWCC 1 cut(s) 183
Eco57I CTGAAG 3 cut(s) 269, 456, 844
Eco88I CYCGRG 1 cut(s) 32
Eco91I GGTNACC 1 cut(s) 742
EcoO109I RGGNCCY 1 cut(s) 827
EcoO65I GGTNACC 1 cut(s) 742
EcoRII CCWGG 2 cut(s) 9, 712
EcoRV GATATC 1 cut(s) 584
EcoT22I ATGCAT 1 cut(s) 272
EcoT38I GRGCYC 2 cut(s) 432, 796
Esp3I CGTCTC 1 cut(s) 29
FaeI CATG 2 cut(s) 482, 565
FaiI YATR 9 cut(s) 29, 51, 480, 518, 563, 798, 809, 875, 916
FaqI GGGAC 1 cut(s) 481
FatI CATG 2 cut(s) 478, 561
FauI CCCGC 1 cut(s) 446
FblI GTMKAC 2 cut(s) 375, 762
Fnu4HI GCNGC 2 cut(s) 133, 724
FokI GGATG 8 cut(s) 279, 365, 371, 376, 615, 644, 729, 877
FriOI GRGCYC 2 cut(s) 432, 796
Fsp4HI GCNGC 2 cut(s) 133, 724
FspBI CTAG 2 cut(s) 500, 893
GluI GCNGC 2 cut(s) 133, 724
GsaI CCCAGC 1 cut(s) 429
HaeIII GGCC 7 cut(s) 126, 147, 208, 527, 627, 656, 829
HapII CCGG 2 cut(s) 168, 186
Hin1II CATG 2 cut(s) 482, 565
HinfI GANTC 3 cut(s) 439, 617, 709
HpaII CCGG 2 cut(s) 168, 186
HphI GGTGA 3 cut(s) 323, 356, 767
Hpy166II GTNNAC 4 cut(s) 376, 485, 546, 763
Hpy188I TCNGA 4 cut(s) 89, 117, 288, 444
Hpy188III TCNNGA 2 cut(s) 773, 862
Hpy8I GTNNAC 4 cut(s) 376, 485, 546, 763
Hpy99I CGWCG 1 cut(s) 576
HpyAV CCTTC 4 cut(s) 310, 343, 572, 840
HpyCH4III ACNGT 3 cut(s) 335, 380, 550
HpyCH4IV ACGT 1 cut(s) 38
HpyF10VI GCNNNNNNNGC 6 cut(s) 132, 407, 473, 533, 703, 835
HpyF3I CTNAG 4 cut(s) 212, 229, 249, 834
HpySE526I ACGT 1 cut(s) 38
Hsp92II CATG 2 cut(s) 482, 565
Kzo9I GATC 2 cut(s) 682, 778
LmnI GCTCC 3 cut(s) 407, 520, 720
Lsp1109I GCAGC 1 cut(s) 119
LweI GCATC 5 cut(s) 190, 257, 279, 680, 855
MaeI CTAG 2 cut(s) 500, 893
MaeII ACGT 1 cut(s) 38
MaeIII GTNAC 3 cut(s) 76, 555, 742
MalI GATC 2 cut(s) 684, 780
MbiI CCGCTC 1 cut(s) 723
MboI GATC 2 cut(s) 682, 778
MboII GAAGA 1 cut(s) 209
MhlI GDGCHC 4 cut(s) 157, 432, 548, 796
MlsI TGGCCA 1 cut(s) 656
MluCI AATT 5 cut(s) 322, 670, 847, 897, 905
MluNI TGGCCA 1 cut(s) 656
MlyI GAGTC 1 cut(s) 433
MnlI CCTC 7 cut(s) 158, 166, 219, 355, 407, 498, 769
Mox20I TGGCCA 1 cut(s) 656
Mph1103I ATGCAT 1 cut(s) 272
MscI TGGCCA 1 cut(s) 656
MseI TTAA 3 cut(s) 294, 326, 369
MslI CAYNNNNRTG 1 cut(s) 551
Msp20I TGGCCA 1 cut(s) 656
MspI CCGG 2 cut(s) 168, 186
MspR9I CCNGG 2 cut(s) 11, 714
MvaI CCWGG 2 cut(s) 11, 714
MwoI GCNNNNNNNGC 6 cut(s) 132, 407, 473, 533, 703, 835
NdeII GATC 2 cut(s) 682, 778
NlaIII CATG 2 cut(s) 482, 565
NlaIV GGNNCC 2 cut(s) 184, 828
NmuCI GTSAC 1 cut(s) 555
NsiI ATGCAT 1 cut(s) 272
NspI RCATGY 1 cut(s) 565
PaeI GCATGC 1 cut(s) 565
PceI AGGCCT 1 cut(s) 208
PfeI GAWTC 2 cut(s) 617, 709
PfoI TCCNGGA 1 cut(s) 712
PkrI GCNGC 2 cut(s) 134, 725
PleI GAGTC 1 cut(s) 433
PpsI GAGTC 1 cut(s) 433
Psp6I CCWGG 2 cut(s) 9, 712
PspEI GGTNACC 1 cut(s) 742
PspFI CCCAGC 1 cut(s) 425
PspGI CCWGG 2 cut(s) 9, 712
PspN4I GGNNCC 2 cut(s) 184, 828
PspPI GGNCC 3 cut(s) 183, 625, 827
RsaI GTAC 1 cut(s) 507
RsaNI GTAC 1 cut(s) 506
RseI CAYNNNNRTG 1 cut(s) 551
SaqAI TTAA 3 cut(s) 294, 326, 369
SatI GCNGC 2 cut(s) 133, 724
Sau3AI GATC 2 cut(s) 682, 778
Sau96I GGNCC 3 cut(s) 183, 625, 827
SchI GAGTC 1 cut(s) 433
ScrFI CCNGG 2 cut(s) 11, 714
SduI GDGCHC 4 cut(s) 157, 432, 548, 796
SfaNI GCATC 5 cut(s) 190, 257, 279, 680, 855
SinI GGWCC 1 cut(s) 183
SmiI ATTTAAAT 1 cut(s) 327
SmiMI CAYNNNNRTG 1 cut(s) 551
SphI GCATGC 1 cut(s) 565
Sse9I AATT 5 cut(s) 322, 670, 847, 897, 905
SseBI AGGCCT 1 cut(s) 208
SsiI CCGC 2 cut(s) 453, 723
SspMI CTAG 2 cut(s) 500, 893
StuI AGGCCT 1 cut(s) 208
StyD4I CCNGG 2 cut(s) 9, 712
SwaI ATTTAAAT 1 cut(s) 327
TaaI ACNGT 3 cut(s) 335, 380, 550
TaiI ACGT 1 cut(s) 41
TaqI TCGA 1 cut(s) 571
TasI AATT 5 cut(s) 322, 670, 847, 897, 905
TauI GCSGC 1 cut(s) 726
TfiI GAWTC 2 cut(s) 617, 709
Tru1I TTAA 3 cut(s) 294, 326, 369
Tru9I TTAA 3 cut(s) 294, 326, 369
TscAI CASTG 1 cut(s) 553
TseFI GTSAC 1 cut(s) 555
TseI GCWGC 1 cut(s) 132
Tsp45I GTSAC 1 cut(s) 555
TspDTI ATGAA 4 cut(s) 16, 38, 210, 329
TspGWI ACGGA 3 cut(s) 140, 483, 682
TspRI CASTG 1 cut(s) 553
VneI GTGCAC 1 cut(s) 544
VpaK11BI GGWCC 1 cut(s) 183
XapI RAATTY 2 cut(s) 670, 847
XceI RCATGY 1 cut(s) 565
XcmI CCANNNNNNNNNTGG 1 cut(s) 401
XmiI GTMKAC 2 cut(s) 375, 762
XspI CTAG 2 cut(s) 500, 893
Zsp2I ATGCAT 1 cut(s) 272
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.