Rh2CG399400

Non-structural maintenance of chromosomes element 1 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2C
OG0015724
Physical Location & Seq
Forward (+)
54302398 .. 54308896
6499 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2CG399400.1

Sequence Viewer

Length: 858 bp
ATGATTTCTTTGCGTTTATATCTTCCACTGATATTCTTAGCGAATGCAGTTCACTGTGGCACACTGATCAAGTCTCTTCCGGGTTTCTTTGGTGAACTTCCTTTCAACCTCTATACTGGATGGAGACCCCAAAGTAGACCGTTTGTTGCTTTGGTACAGTGGAGACCTTGGTTTCTGGTTTTAATGGCCTCATTTATCAAAATGGTACTGTCTTTTTCACTCTCGGACTACATTCGCATACTGGATGAAGGAGGTACTTATTACTACCCATACTCATGGACAAGGGAAGAGTTGTACCTCACAGCTTATATAGAAAGGGATGGTAAAGGTTCCGACATAATCCATAACATGATGTGGAAAGTGATGATCATTAACATCCCCAAAAGAAGCTGTTCTTCAAATTCTAATGGAGAAGAAGTCAACTTCCCTGACAAGGCCAAGGTTGACAAAAATCTGAAGTTCATAATCTTAATTTTTATCTTTGTATGTGTGCGGTGTGTAATCATATGCAATTGTGCATTGTGTGCACGCTGGAGCATAATCCTCGTCTACAGATGTATAGAAGCAATAGTGCAAGAAGCTGCTCAAGGAACCATATCTAGCAATGACGCTCTTAATCTACGATTAGAGAATCAGGTAGTGTTATGCCAGAAAGAGGGTTGTTCGGTTCAAATTCATCAGTACTGCCTAGGAAAACTGTTTGCTAAGAAAAAGGGTGAAAGAGTTTGTCCAAGTTGTGGTACTCAATGGCAACATACAAGACCAAAAGCAGAACCTGTAGAAGATGATGAGCCAGATTGCGCTACTCAGAGCCAACCACCTGTGGAAGCCAAGAGAAAGAAGCTGGGAACAAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SMC_Nse1 zf-RING-like

Protein Analysis

285

Amino Acids

32.62

Weight (kDa)

8.79

Isoelectric Point (pI)

41.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-RING-like PF08746 214 - 246 3.4e-06 RING-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 737
AccI GTMKAC 2 cut(s) 136, 549
AciI CCGC 1 cut(s) 493
AcsI RAATTY 2 cut(s) 400, 672
AcuI CTGAAG 1 cut(s) 476
AfaI GTAC 6 cut(s) 156, 207, 256, 296, 683, 742
AfiI CCNNNNNNNGG 3 cut(s) 433, 655, 737
AgsI TTSAA 3 cut(s) 106, 399, 671
AluBI AGCT 4 cut(s) 305, 390, 581, 844
AluI AGCT 4 cut(s) 305, 390, 581, 844
Alw21I GWGCWC 1 cut(s) 529
Alw26I GTCTC 3 cut(s) 78, 118, 157
Alw44I GTGCAC 1 cut(s) 525
AlwNI CAGNNNCTG 1 cut(s) 776
AoxI GGCC 2 cut(s) 186, 435
ApaLI GTGCAC 1 cut(s) 525
ApeKI GCWGC 1 cut(s) 581
ApoI RAATTY 2 cut(s) 400, 672
Asp700I GAANNNNTTC 1 cut(s) 391
AspA2I CCTAGG 1 cut(s) 688
AspLEI GCGC 1 cut(s) 803
AsuC2I CCSGG 1 cut(s) 81
AsuHPI GGTGA 2 cut(s) 104, 728
AvrII CCTAGG 1 cut(s) 688
BaeGI GKGCMC 1 cut(s) 529
Bbv12I GWGCWC 1 cut(s) 529
BbvI GCAGC 1 cut(s) 568
BccI CCATC 2 cut(s) 114, 314
BcgI CGANNNNNNTGC 2 cut(s) 526, 560
BclI TGATCA 2 cut(s) 66, 366
BcnI CCSGG 1 cut(s) 81
BcoDI GTCTC 3 cut(s) 78, 118, 157
BfaI CTAG 2 cut(s) 600, 689
BfmI CTRYAG 2 cut(s) 550, 777
BisI GCNGC 1 cut(s) 582
BlnI CCTAGG 1 cut(s) 688
BlsI GCNGC 1 cut(s) 583
BmcAI AGTACT 1 cut(s) 683
Bme1390I CCNGG 1 cut(s) 81
BmiI GGNNCC 2 cut(s) 331, 592
BmrFI CCNGG 1 cut(s) 81
BpmI CTGGAG 1 cut(s) 553
BpuEI CTTGAG 1 cut(s) 570
BpuMI CCSGG 1 cut(s) 81
BsaI GGTCTC 2 cut(s) 118, 157
BsaJI CCNNGG 3 cut(s) 167, 438, 688
BsaXI ACNNNNNCTCC 2 cut(s) 402, 432
Bsc4I CCNNNNNNNGG 3 cut(s) 433, 655, 737
Bse1I ACTGG 2 cut(s) 121, 246
Bse3DI GCAATG 1 cut(s) 610
BseDI CCNNGG 3 cut(s) 167, 438, 688
BseGI GGATG 4 cut(s) 125, 250, 325, 375
BseLI CCNNNNNNNGG 3 cut(s) 433, 655, 737
BseMI GCAATG 1 cut(s) 610
BseMII CTCAG 1 cut(s) 821
BseNI ACTGG 2 cut(s) 121, 246
BseSI GKGCMC 1 cut(s) 529
BseXI GCAGC 1 cut(s) 568
BseYI CCCAGC 1 cut(s) 844
BshFI GGCC 2 cut(s) 188, 437
BsiHKAI GWGCWC 1 cut(s) 529
BsiSI CCGG 1 cut(s) 80
BslI CCNNNNNNNGG 3 cut(s) 433, 655, 737
BsmAI GTCTC 3 cut(s) 78, 118, 157
BsmI GAATGC 1 cut(s) 49
BsnI GGCC 2 cut(s) 188, 437
Bso31I GGTCTC 2 cut(s) 118, 157
Bsp1286I GDGCHC 1 cut(s) 529
Bsp143I GATC 2 cut(s) 66, 366
BspACI CCGC 1 cut(s) 493
BspANI GGCC 2 cut(s) 188, 437
BspCNI CTCAG 1 cut(s) 820
BspLI GGNNCC 2 cut(s) 331, 592
BspTNI GGTCTC 2 cut(s) 118, 157
BsrDI GCAATG 1 cut(s) 610
BsrI ACTGG 2 cut(s) 121, 246
BssECI CCNNGG 3 cut(s) 167, 438, 688
BssMI GATC 2 cut(s) 66, 366
BssT1I CCWWGG 3 cut(s) 167, 438, 688
Bst4CI ACNGT 5 cut(s) 56, 141, 159, 210, 699
Bst6I CTCTTC 2 cut(s) 81, 282
BstAPI GCANNNNNTGC 1 cut(s) 524
BstC8I GCNNGC 1 cut(s) 529
BstDEI CTNAG 3 cut(s) 37, 705, 807
BstF5I GGATG 4 cut(s) 125, 250, 325, 375
BstHHI GCGC 1 cut(s) 803
BstKTI GATC 2 cut(s) 69, 369
BstMAI GTCTC 3 cut(s) 78, 118, 157
BstMBI GATC 2 cut(s) 66, 366
BstMWI GCNNNNNNNGC 1 cut(s) 524
BstSCI CCNGG 1 cut(s) 79
BstSFI CTRYAG 2 cut(s) 550, 777
BstSLI GKGCMC 1 cut(s) 529
BstV1I GCAGC 1 cut(s) 568
BstXI CCANNNNNNTGG 1 cut(s) 276
BsuRI GGCC 2 cut(s) 188, 437
BtsCI GGATG 4 cut(s) 125, 250, 325, 375
BtsIMutI CAGTG 4 cut(s) 26, 52, 62, 164
Cac8I GCNNGC 1 cut(s) 529
CaiI CAGNNNCTG 1 cut(s) 776
CfoI GCGC 1 cut(s) 803
CseI GACGC 1 cut(s) 617
Csp6I GTAC 6 cut(s) 155, 206, 255, 295, 682, 741
CviAII CATG 2 cut(s) 276, 349
CviJI RGCY 9 cut(s) 188, 305, 390, 437, 581, 793, 813, 830, 844
CviKI_1 RGCY 9 cut(s) 188, 305, 390, 437, 581, 793, 813, 830, 844
CviQI GTAC 6 cut(s) 155, 206, 255, 295, 682, 741
DdeI CTNAG 3 cut(s) 37, 705, 807
DpnI GATC 2 cut(s) 68, 368
DpnII GATC 2 cut(s) 66, 366
Eam1104I CTCTTC 2 cut(s) 81, 282
EarI CTCTTC 2 cut(s) 81, 282
Eco130I CCWWGG 3 cut(s) 167, 438, 688
Eco31I GGTCTC 2 cut(s) 118, 157
Eco57I CTGAAG 1 cut(s) 476
EcoT14I CCWWGG 3 cut(s) 167, 438, 688
ErhI CCWWGG 3 cut(s) 167, 438, 688
FaeI CATG 2 cut(s) 279, 352
FalI AAGNNNNNCTT 2 cut(s) 379, 411
FatI CATG 2 cut(s) 275, 348
FauNDI CATATG 1 cut(s) 506
FbaI TGATCA 2 cut(s) 66, 366
FblI GTMKAC 2 cut(s) 136, 549
Fnu4HI GCNGC 1 cut(s) 582
FokI GGATG 4 cut(s) 132, 257, 332, 362
Fsp4HI GCNGC 1 cut(s) 582
FspBI CTAG 2 cut(s) 600, 689
GlaI GCGC 1 cut(s) 802
GluI GCNGC 1 cut(s) 582
GsaI CCCAGC 1 cut(s) 848
GsuI CTGGAG 1 cut(s) 553
HaeIII GGCC 2 cut(s) 188, 437
HapII CCGG 1 cut(s) 80
HgaI GACGC 1 cut(s) 617
HhaI GCGC 1 cut(s) 803
Hin1II CATG 2 cut(s) 279, 352
Hin6I GCGC 1 cut(s) 801
HinP1I GCGC 1 cut(s) 801
HincII GTYRAC 2 cut(s) 421, 445
HindII GTYRAC 2 cut(s) 421, 445
HinfI GANTC 1 cut(s) 631
HpaII CCGG 1 cut(s) 80
HphI GGTGA 2 cut(s) 104, 728
Hpy166II GTNNAC 7 cut(s) 52, 95, 137, 421, 445, 527, 550
Hpy188I TCNGA 4 cut(s) 226, 334, 456, 810
Hpy8I GTNNAC 7 cut(s) 52, 95, 137, 421, 445, 527, 550
HpyAV CCTTC 1 cut(s) 242
HpyCH4III ACNGT 5 cut(s) 56, 141, 159, 210, 699
HpyCH4V TGCA 5 cut(s) 47, 510, 518, 527, 574
HpyF10VI GCNNNNNNNGC 1 cut(s) 524
HpyF3I CTNAG 3 cut(s) 37, 705, 807
Hsp92II CATG 2 cut(s) 279, 352
HspAI GCGC 1 cut(s) 801
Ksp22I TGATCA 2 cut(s) 66, 366
Kzo9I GATC 2 cut(s) 66, 366
LmnI GCTCC 1 cut(s) 534
Lsp1109I GCAGC 1 cut(s) 568
MaeI CTAG 2 cut(s) 600, 689
MalI GATC 2 cut(s) 68, 368
MboI GATC 2 cut(s) 66, 366
MboII GAAGA 6 cut(s) 14, 68, 299, 387, 425, 794
MfeI CAATTG 1 cut(s) 511
MhlI GDGCHC 1 cut(s) 529
MluCI AATT 4 cut(s) 400, 471, 511, 672
MmeI TCCRAC 1 cut(s) 357
MnlI CCTC 6 cut(s) 119, 199, 245, 308, 554, 649
MroXI GAANNNNTTC 1 cut(s) 391
MseI TTAA 4 cut(s) 182, 372, 470, 615
MslI CAYNNNNRTG 1 cut(s) 274
MspI CCGG 1 cut(s) 80
MspR9I CCNGG 1 cut(s) 81
MunI CAATTG 1 cut(s) 511
Mva1269I GAATGC 1 cut(s) 49
MwoI GCNNNNNNNGC 1 cut(s) 524
NciI CCSGG 1 cut(s) 81
NdeI CATATG 1 cut(s) 506
NdeII GATC 2 cut(s) 66, 366
NlaIII CATG 2 cut(s) 279, 352
NlaIV GGNNCC 2 cut(s) 331, 592
PctI GAATGC 1 cut(s) 49
PdmI GAANNNNTTC 1 cut(s) 391
PfeI GAWTC 1 cut(s) 631
PflMI CCANNNNNTGG 1 cut(s) 737
PkrI GCNGC 1 cut(s) 583
PspFI CCCAGC 1 cut(s) 844
PspN4I GGNNCC 2 cut(s) 331, 592
PstNI CAGNNNCTG 1 cut(s) 776
RsaI GTAC 6 cut(s) 156, 207, 256, 296, 683, 742
RsaNI GTAC 6 cut(s) 155, 206, 255, 295, 682, 741
RseI CAYNNNNRTG 1 cut(s) 274
SaqAI TTAA 4 cut(s) 182, 372, 470, 615
SatI GCNGC 1 cut(s) 582
Sau3AI GATC 2 cut(s) 66, 366
ScaI AGTACT 1 cut(s) 683
ScrFI CCNGG 1 cut(s) 81
SduI GDGCHC 1 cut(s) 529
SfcI CTRYAG 2 cut(s) 550, 777
SmiMI CAYNNNNRTG 1 cut(s) 274
SmlI CTYRAG 1 cut(s) 585
SmoI CTYRAG 1 cut(s) 585
Sse9I AATT 4 cut(s) 400, 471, 511, 672
SsiI CCGC 1 cut(s) 493
SspMI CTAG 2 cut(s) 600, 689
StyD4I CCNGG 1 cut(s) 79
StyI CCWWGG 3 cut(s) 167, 438, 688
TaaI ACNGT 5 cut(s) 56, 141, 159, 210, 699
TasI AATT 4 cut(s) 400, 471, 511, 672
TatI WGTACW 1 cut(s) 681
TfiI GAWTC 1 cut(s) 631
Tru1I TTAA 4 cut(s) 182, 372, 470, 615
Tru9I TTAA 4 cut(s) 182, 372, 470, 615
TscAI CASTG 4 cut(s) 33, 59, 69, 164
TseI GCWGC 1 cut(s) 581
TspDTI ATGAA 3 cut(s) 261, 451, 665
TspRI CASTG 4 cut(s) 33, 59, 69, 164
Van91I CCANNNNNTGG 1 cut(s) 737
VneI GTGCAC 1 cut(s) 525
XapI RAATTY 2 cut(s) 400, 672
XmaJI CCTAGG 1 cut(s) 688
XmiI GTMKAC 2 cut(s) 136, 549
XmnI GAANNNNTTC 1 cut(s) 391
XspI CTAG 2 cut(s) 600, 689
ZrmI AGTACT 1 cut(s) 683
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.